Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0002968

Physical Location & Seq

Genomic Coordinates Reverse (-)

11429546 .. 11433424

Gene Structure 3879 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032150

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2454 bp

ATGGTCTCTATTCGTAGCTACATTCGAACCGGAGGAGCAGCTCGGCTCAGACCTCATAGCTCGTGTGAGTCCACCACATCTTTCTCTCAGTACCCTTTAACTTTTGTTGAGCTCCCTCAATTTTTCTCATCAGTTAGCTCAAGAAACTCAAGTTCAGATTTTTGCTCTGGAATTAGACCTCATTCTGTTGAAAGTTATGATTTTGATGATTCTAATAATGGGAAAGGTAGTTTTAGGAGAATGGGTAATCGAAATATGGTGGGTTTGAGTTTCTTAGGAGTTGCCCAGAGTGATTGTGAAAACCCCCAAAGTGAAAGTTTCAAACTTTTTGAAGTTTCAGATTGCGAAGAAGGGAATAATAGTGATGGTGGTGGTGATGATGATGATGAGTTAATGATGTTGAATTCTAATAGGGGTTGTGAGCAAAAAGAGAATTTGGGTAGAGTTGAAGGAGATGGAGATGAGGATGAATATAGACACCCTTTAGTGAGAGAGGTTTGTAGGTTAATTGAGCTTAGAACAGCTTGGACCCCAAAGTTTGAAGGTGAGTTAAGGCACTTGTTCCGAAGCCTGAAGCCGAAGCAGGTCTGTGCCGTTTTGAAGTCTCAGGCTGATGAAAGAGTTGCTTTGAAGATTTTTTACTGGGCTGATAGGCAGTGGCGGTATAAGCATGACCCGATTGTGTACTATGCAATGCTTGAGGTGCTTGGCAAGACTAAGTTGTGCCAAGGTGCTAGGAGGGTTCTTAGGTTAATGGCCCGTAGGCGGATTGAGCTTCGTTCTGAAGCTTTTGGCCATGTGATGGTGTCATATAGTCGAGCGGGCAAGTTGAGGCACGCGTTGCGTGTGTTGACTTTGATGCAGAAAGCGGGGCTCGAGCTTGATTTGTCAATGTGTAATACTGCAATTTATGTTTTGGTTAAGGGTAATAAGTTGGAGAAGGCGTTGAGGACCTTGGAAAGGATGAAGCTTGTTGGGATTGCACCAAATGTAGTCACTTATAACTGTTTGATAAAGGGGTATTGTGATCTGCATCGTGTAGAAGATGCAATCCAGGTGATTGATGAAATGCCGAGGAAGGGTTGTGCTCCTGATAAAGTTAGTTACTATACTGTGATGGATTTCCTTTGTAAGGAGAAGAGGGTCAAAGGAGTGAGAGACTTGATGGAGAAGATGATCAAGGATGGTGGGATGTTACCAGACCAGGTCACTTATAATAATCTTGTCCATGTGCTTTGTAAGCATGGCTATGGTGATGAGGCACTTGAATTTTTAAGGGAAGCCGAAGAGAAGGGATTTCGGTTTGATAAGGTTGGGTATAGTGCAATTGTACATTCATTTTGTAAGGATGGTCGGACTAACATGGCAAAAGAAATTGTTAGTGAAATGACTTCAAAAGGTTGCATCCCAGATGTTGTAACATACACTGCTGTTCTGAATGGGTATTGTCGACTTGGGAAGGTAGATCAAGCTAAAAAGATGCTTCGGCACATGCATAAGCATGGTTGCAAGCCAAATACTGTATCATATACGGCCTTATTAAATGGGCTTTGTCTTGGTGGGAACTCATTGGAGGCAAGAGAGATGATGAACACGAGTGAGGAAGAATGGTGGACACCAAATGCCGTCACTTATAGTGTTCTGATGCATGGGTTCCGGAGGGAAGGGAAATTGGTTGAGGCTTGTGATATTGTCAAAGAGATGATTAAAAAGGGATTTTTCCCTACCCCAGTTGAAATTAATCTCTTAATTCAGTCTCTTTGCCAAGAGGGGAAGATGGATGAGGCTAAACGTTTCATGGAGGAGTGTCTAAATAAAGGTTGTGCTGTTAATGTTGTCAATTTTACTACTGTTATTCATGGGTATTGTCAAAAAGATGACTTGGAAGCTGCTCTGTCGTTGCTTGATGACATGTATCTCAGCAACAAACACCCAGATGCAGTAACTTATACAGCAATGATCAGTGCATTGGGAAAGAAAGGTAGAATACAAGACGCAACAGAACTTATGATGAAGATGCTGAATAAGGGTATTGACCCGACTCCCGTCACATACAGGACGATCATCCACTGGTATTGTAAGATGAGTAGGGTGGATGATTTGTTGAAATTATTGGAGAAGATGTTCTTAAGGCAAAATTGCAAAACAGCATATAATCAAGTGATTGAGAAGCTTTGTAGTTTTGGGAACTTCGAGGACGCTGATAAACTATTGGGTAAGGTGTTGAGAACAGCTTCAAGAGTTGATGCTAAAACATGCCACGTTGTTATGGATGGTTATTTGAGAAAAGGGATACCTCTGTCAGCATACAAAGTGGCTTGTCGAATGTTCAATCGGAATCTGATTCCTGATTTAAAGTTGTGTGAGAAGGTGACGAAGAGACTGATGCTAGAAGGAAACTCCAAAGAGGCAGACAACCTTATGTTACGGACTCTGACACGGACCTGGGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016070 GO:0016787 GO:0016788 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

818

Amino Acids

92.92

Weight (kDa)

8.68

Isoelectric Point (pI)

32.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	233 - 323	4.7e-07	Fungal tetratrico peptide repeats
PPR_long	PF17177	264 - 427	6.1e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	283 - 341	4.9e-12	Pentatricopeptide repeat domain
PPR_2	PF13041	297 - 343	2.7e-11	PPR repeat family
PPR_1	PF12854	326 - 357	1.1e-11	PPR repeat
PPR_2	PF13041	329 - 378	7.3e-17	PPR repeat family
PPR	PF01535	332 - 362	1.1e-07	PPR repeat
PPR_long	PF17177	385 - 555	4.4e-12	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	397 - 427	2.8e-08	PPR repeat
PPR_2	PF13041	400 - 449	5.7e-10	PPR repeat family
PPR	PF01535	403 - 433	1.5e-06	PPR repeat
PPR_3	PF13812	425 - 481	2.1e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	437 - 484	3.5e-13	PPR repeat family
PPR_3	PF13812	459 - 515	6.8e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	466 - 498	1.8e-12	PPR repeat
PPR_2	PF13041	470 - 518	8.7e-19	PPR repeat family
PPR	PF01535	473 - 503	6.3e-08	PPR repeat
PPR_1	PF12854	501 - 531	1.3e-06	PPR repeat
PPR_2	PF13041	540 - 576	1.5e-06	PPR repeat family
TPR_24	PF23276	541 - 635	1e-05	Fungal tetratrico peptide repeats
PPR	PF01535	543 - 573	2.9e-06	PPR repeat
PPR_1	PF12854	606 - 638	4.4e-08	PPR repeat
PPR_2	PF13041	611 - 659	1.9e-13	PPR repeat family
PPR_3	PF13812	633 - 694	2.8e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	644 - 673	5.4e-07	PPR repeat
PPR_2	PF13041	645 - 694	2.9e-14	PPR repeat family
PPR	PF01535	648 - 678	6.1e-07	PPR repeat
PPR_1	PF12854	676 - 708	8.2e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002968)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30290 AT1G30290
fragaria_vesca	FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050 FvH4_3g05050
malus_domestica	MD10G1258300.v1.1
prunus_persica	Prupe.4G085500_v2.0.a1 Prupe.4G085500_v2.0.a1
pyrus_communis	pycom05g26000 pycom10g21460
rosa_chinensis	RchiOBHm_Chr3g0490541 RchiOBHm_Chr3g0490551 RchiOBHm_Chr5g0014911
rosa_laevigata	RLG00000022885 RLG00000032150
rosa_multiflora	Rmu_sc0003862.1_g000007 Rmu_sc0013610.1_g000002
rosa_roxburghii	Rroxscaffold_1G00061660 Rroxscaffold_6G00393420
rosa_rugosa	Rorug03G0243900 Rorug05G0021400
rosa_samantha	Rh3AG293300 Rh3AG293400 Rh3BG329800 Rh3CG327300 Rh3CG327400 Rh3DG326700 Rh5AG117100 Rh5BG114100 Rh5CG125600 Rh5DG113500
rosa_wichuraiana	Rw3G025970 Rw5G010080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1002, 1215
Acc36I	ACCTGC	1 cut(s)	574
AccB7I	CCANNNNNTGG	1 cut(s)	802
AccBSI	CCGCTC	1 cut(s)	821
AccI	GTMKAC	1 cut(s)	1450
AccII	CGCG	1 cut(s)	839
AccIII	TCCGGA	1 cut(s)	1656
AciI	CCGC	4 cut(s)	661, 766, 821, 869
AclI	AACGTT	1 cut(s)	1792
AcoI	YGGCCR	1 cut(s)	793
AcsI	RAATTY	3 cut(s)	403, 433, 1268
AcuI	CTGAAG	2 cut(s)	593, 804
AfaI	GTAC	3 cut(s)	92, 686, 1332
AfiI	CCNNNNNNNGG	5 cut(s)	765, 802, 960, 1079, 1132
AflII	CTTAAG	1 cut(s)	2128
AflIII	ACRYGT	2 cut(s)	837, 1911
AjnI	CCWGG	3 cut(s)	1053, 1203, 2444
AloI	GAACNNNNNNTCC	2 cut(s)	2108, 2140
Alw21I	GWGCWC	2 cut(s)	114, 1090
Alw26I	GTCTC	5 cut(s)	10, 609, 1152, 1761, 2374
Ama87I	CYCGRG	1 cut(s)	875
Aor13HI	TCCGGA	1 cut(s)	1656
AoxI	GGCC	3 cut(s)	756, 793, 1533
ApeKI	GCWGC	2 cut(s)	38, 1889
ApoI	RAATTY	3 cut(s)	403, 433, 1268
AseI	ATTAAT	1 cut(s)	1740
Asp700I	GAANNNNTTC	2 cut(s)	2123, 2233
AspS9I	GGNCC	4 cut(s)	528, 757, 951, 2442
AsuHPI	GGTGA	5 cut(s)	386, 557, 1069, 1265, 2383
AsuII	TTCGAA	1 cut(s)	25
AvaI	CYCGRG	1 cut(s)	875
AvaII	GGWCC	3 cut(s)	528, 951, 2442
BaeI	ACNNNNGTAYC	2 cut(s)	2285, 2318
BalI	TGGCCA	1 cut(s)	795
BanII	GRGCYC	2 cut(s)	114, 876
BarI	GAAGNNNNNNTAC	2 cut(s)	623, 655
BauI	CACGAG	2 cut(s)	61, 1594
Bbv12I	GWGCWC	2 cut(s)	114, 1090
BbvI	GCAGC	2 cut(s)	50, 1876
BccI	CCATC	9 cut(s)	359, 449, 796, 1111, 1159, 1178, 1343, 1771, 2267
BceAI	ACGGC	3 cut(s)	578, 1548, 1610
BciT130I	CCWGG	3 cut(s)	1055, 1205, 2446
BciVI	GTATCC	1 cut(s)	2286
BclI	TGATCA	2 cut(s)	1176, 1959
BcoDI	GTCTC	5 cut(s)	10, 609, 1152, 1761, 2374
BfaI	CTAG	2 cut(s)	735, 2390
BfrI	CTTAAG	1 cut(s)	2128
BfuAI	ACCTGC	1 cut(s)	574
BfuI	GTATCC	1 cut(s)	2286
BisI	GCNGC	2 cut(s)	39, 1890
BlsI	GCNGC	2 cut(s)	40, 1891
Bme1390I	CCNGG	3 cut(s)	1055, 1205, 2446
Bme18I	GGWCC	3 cut(s)	528, 951, 2442
BmeT110I	CYCGRG	1 cut(s)	875
BmgT120I	GGNCC	4 cut(s)	528, 757, 951, 2442
BmiI	GGNNCC	2 cut(s)	530, 1655
BmrFI	CCNGG	3 cut(s)	1055, 1205, 2446
BmrI	ACTGGG	2 cut(s)	652, 1724
BmuI	ACTGGG	2 cut(s)	652, 1724
BoxI	GACNNNNGTC	1 cut(s)	2045
Bpu14I	TTCGAA	1 cut(s)	25
BpuEI	CTTGAG	3 cut(s)	124, 133, 719
BsaBI	GATNNNNATC	1 cut(s)	1032
BsaI	GGTCTC	1 cut(s)	10
BsaJI	CCNNGG	4 cut(s)	727, 954, 1073, 2445
BsaWI	WCCGGW	2 cut(s)	29, 1656
BsaXI	ACNNNNNCTCC	6 cut(s)	229, 259, 929, 959, 2108, 2138
Bsc4I	CCNNNNNNNGG	5 cut(s)	765, 802, 960, 1079, 1132
Bse1I	ACTGG	3 cut(s)	647, 1730, 2075
Bse3DI	GCAATG	2 cut(s)	699, 1962
Bse8I	GATNNNNATC	1 cut(s)	1032
BseAI	TCCGGA	1 cut(s)	1656
BseBI	CCWGG	3 cut(s)	1055, 1205, 2446
BseDI	CCNNGG	4 cut(s)	727, 954, 1073, 2445
BseJI	GATNNNNATC	1 cut(s)	1032
BseLI	CCNNNNNNNGG	5 cut(s)	765, 802, 960, 1079, 1132
BseMI	GCAATG	2 cut(s)	699, 1962
BseMII	CTCAG	4 cut(s)	61, 101, 620, 1933
BseNI	ACTGG	3 cut(s)	647, 1730, 2075
BseRI	GAGGAG	2 cut(s)	48, 1817
BseXI	GCAGC	2 cut(s)	50, 1876
Bsh1236I	CGCG	1 cut(s)	839
BshFI	GGCC	3 cut(s)	758, 795, 1535
BsiHKAI	GWGCWC	2 cut(s)	114, 1090
BsiHKCI	CYCGRG	1 cut(s)	875
BsiSI	CCGG	2 cut(s)	30, 1657
BslI	CCNNNNNNNGG	5 cut(s)	765, 802, 960, 1079, 1132
BsmAI	GTCTC	5 cut(s)	10, 609, 1152, 1761, 2374
BsnI	GGCC	3 cut(s)	758, 795, 1535
Bso31I	GGTCTC	1 cut(s)	10
BsoBI	CYCGRG	1 cut(s)	875
Bsp119I	TTCGAA	1 cut(s)	25
Bsp1286I	GDGCHC	3 cut(s)	114, 876, 1090
Bsp13I	TCCGGA	1 cut(s)	1656
Bsp1407I	TGTACA	1 cut(s)	1330
Bsp143I	GATC	5 cut(s)	1027, 1176, 1465, 1959, 2061
BspACI	CCGC	4 cut(s)	661, 766, 821, 869
BspANI	GGCC	3 cut(s)	758, 795, 1535
BspCNI	CTCAG	4 cut(s)	60, 100, 619, 1932
BspEI	TCCGGA	1 cut(s)	1656
BspFNI	CGCG	1 cut(s)	839
BspLI	GGNNCC	2 cut(s)	530, 1655
BspMI	ACCTGC	1 cut(s)	574
BspT104I	TTCGAA	1 cut(s)	25
BspTI	CTTAAG	1 cut(s)	2128
BspTNI	GGTCTC	1 cut(s)	10
BsrBI	CCGCTC	1 cut(s)	821
BsrDI	GCAATG	2 cut(s)	699, 1962
BsrGI	TGTACA	1 cut(s)	1330
BsrI	ACTGG	3 cut(s)	647, 1730, 2075
BssECI	CCNNGG	4 cut(s)	727, 954, 1073, 2445
BssMI	GATC	5 cut(s)	1027, 1176, 1465, 1959, 2061
BssSI	CACGAG	2 cut(s)	61, 1594
BssT1I	CCWWGG	2 cut(s)	727, 954
Bst2BI	CACGAG	2 cut(s)	61, 1594
Bst2UI	CCWGG	3 cut(s)	1055, 1205, 2446
Bst4CI	ACNGT	4 cut(s)	1007, 1114, 1522, 1852
Bst6I	CTCTTC	3 cut(s)	1133, 1281, 2372
BstAFI	CTTAAG	1 cut(s)	2128
BstAPI	GCANNNNNTGC	1 cut(s)	841
BstAUI	TGTACA	1 cut(s)	1330
BstBI	TTCGAA	1 cut(s)	25
BstC8I	GCNNGC	3 cut(s)	823, 837, 1511
BstDEI	CTNAG	8 cut(s)	47, 87, 274, 515, 606, 717, 746, 1919
BstENI	CCTNNNNNAGG	2 cut(s)	958, 1130
BstFNI	CGCG	1 cut(s)	839
BstKTI	GATC	5 cut(s)	1030, 1179, 1468, 1962, 2064
BstMAI	GTCTC	5 cut(s)	10, 609, 1152, 1761, 2374
BstMBI	GATC	5 cut(s)	1027, 1176, 1465, 1959, 2061
BstMWI	GCNNNNNNNGC	5 cut(s)	667, 703, 772, 841, 1240
BstNI	CCWGG	3 cut(s)	1055, 1205, 2446
BstNSI	RCATGY	3 cut(s)	1495, 1915, 2259
BstPAI	GACNNNNGTC	1 cut(s)	2045
BstSCI	CCNGG	3 cut(s)	1053, 1203, 2444
BstUI	CGCG	1 cut(s)	839
BstV1I	GCAGC	2 cut(s)	50, 1876
BsuI	GTATCC	1 cut(s)	2286
BsuRI	GGCC	3 cut(s)	758, 795, 1535
BtsI	GCAGTG	2 cut(s)	662, 1425
BtsIMutI	CAGTG	4 cut(s)	662, 1425, 1969, 2068
BveI	ACCTGC	1 cut(s)	574
Cac8I	GCNNGC	3 cut(s)	823, 837, 1511
Cfr13I	GGNCC	4 cut(s)	528, 757, 951, 2442
CseI	GACGC	2 cut(s)	2003, 2207
CsiI	ACCWGGT	1 cut(s)	1203
Csp6I	GTAC	3 cut(s)	91, 685, 1331
CspCI	CAANNNNNGTGG	4 cut(s)	1168, 1203, 2057, 2092
CviQI	GTAC	3 cut(s)	91, 685, 1331
DdeI	CTNAG	8 cut(s)	47, 87, 274, 515, 606, 717, 746, 1919
DpnI	GATC	5 cut(s)	1029, 1178, 1467, 1961, 2063
DpnII	GATC	5 cut(s)	1027, 1176, 1465, 1959, 2061
DraI	TTTAAA	1 cut(s)	2355
EaeI	YGGCCR	1 cut(s)	793
Eam1104I	CTCTTC	3 cut(s)	1133, 1281, 2372
EarI	CTCTTC	3 cut(s)	1133, 1281, 2372
EciI	GGCGGA	1 cut(s)	781
Ecl136II	GAGCTC	1 cut(s)	112
Eco130I	CCWWGG	2 cut(s)	727, 954
Eco24I	GRGCYC	2 cut(s)	114, 876
Eco31I	GGTCTC	1 cut(s)	10
Eco47I	GGWCC	3 cut(s)	528, 951, 2442
Eco53kI	GAGCTC	1 cut(s)	112
Eco57I	CTGAAG	2 cut(s)	593, 804
Eco88I	CYCGRG	1 cut(s)	875
EcoICRI	GAGCTC	1 cut(s)	112
EcoNI	CCTNNNNNAGG	2 cut(s)	958, 1130
EcoO109I	RGGNCCY	1 cut(s)	951
EcoRI	GAATTC	1 cut(s)	403
EcoRII	CCWGG	3 cut(s)	1053, 1203, 2444
EcoT14I	CCWWGG	2 cut(s)	727, 954
EcoT22I	ATGCAT	2 cut(s)	1497, 1650
EcoT38I	GRGCYC	2 cut(s)	114, 876
ErhI	CCWWGG	2 cut(s)	727, 954
FalI	AAGNNNNNCTT	4 cut(s)	610, 642, 2111, 2143
FauI	CCCGC	2 cut(s)	814, 862
FbaI	TGATCA	2 cut(s)	1176, 1959
FblI	GTMKAC	1 cut(s)	1450
Fnu4HI	GCNGC	2 cut(s)	39, 1890
FriOI	GRGCYC	2 cut(s)	114, 876
Fsp4HI	GCNGC	2 cut(s)	39, 1890
FspBI	CTAG	2 cut(s)	735, 2390
GluI	GCNGC	2 cut(s)	39, 1890
HaeIII	GGCC	3 cut(s)	758, 795, 1535
HapII	CCGG	2 cut(s)	30, 1657
HgaI	GACGC	2 cut(s)	2003, 2207
HincII	GTYRAC	2 cut(s)	852, 1451
HindII	GTYRAC	2 cut(s)	852, 1451
HindIII	AAGCTT	3 cut(s)	786, 968, 2171
HinfI	GANTC	6 cut(s)	68, 209, 2041, 2338, 2344, 2431
HpaII	CCGG	2 cut(s)	30, 1657
HphI	GGTGA	5 cut(s)	386, 557, 1069, 1265, 2383
Hpy166II	GTNNAC	5 cut(s)	72, 685, 852, 1451, 1614
Hpy188III	TCNNGA	6 cut(s)	141, 168, 1091, 1657, 2238, 2348
Hpy8I	GTNNAC	5 cut(s)	72, 685, 852, 1451, 1614
HpyCH4III	ACNGT	4 cut(s)	1007, 1114, 1522, 1852
HpyCH4IV	ACGT	2 cut(s)	1792, 2262
HpyF10VI	GCNNNNNNNGC	5 cut(s)	667, 703, 772, 841, 1240
HpyF3I	CTNAG	8 cut(s)	47, 87, 274, 515, 606, 717, 746, 1919
HpySE526I	ACGT	2 cut(s)	1792, 2262
Kpn2I	TCCGGA	1 cut(s)	1656
Ksp22I	TGATCA	2 cut(s)	1176, 1959
Kzo9I	GATC	5 cut(s)	1027, 1176, 1465, 1959, 2061
LmnI	GCTCC	3 cut(s)	35, 117, 1093
Lsp1109I	GCAGC	2 cut(s)	50, 1876
MabI	ACCWGGT	1 cut(s)	1203
MaeI	CTAG	2 cut(s)	735, 2390
MaeII	ACGT	2 cut(s)	1792, 2262
MalI	GATC	5 cut(s)	1029, 1178, 1467, 1961, 2063
MbiI	CCGCTC	1 cut(s)	821
MboI	GATC	5 cut(s)	1027, 1176, 1465, 1959, 2061
MfeI	CAATTG	1 cut(s)	1326
MhlI	GDGCHC	3 cut(s)	114, 876, 1090
MlsI	TGGCCA	1 cut(s)	795
MluI	ACGCGT	1 cut(s)	837
MluNI	TGGCCA	1 cut(s)	795
MlyI	GAGTC	3 cut(s)	77, 2035, 2425
MmeI	TCCRAC	2 cut(s)	915, 1334
Mox20I	TGGCCA	1 cut(s)	795
Mph1103I	ATGCAT	2 cut(s)	1497, 1650
MroI	TCCGGA	1 cut(s)	1656
MroXI	GAANNNNTTC	2 cut(s)	2123, 2233
MscI	TGGCCA	1 cut(s)	795
MslI	CAYNNNNRTG	3 cut(s)	1248, 1500, 1935
Msp20I	TGGCCA	1 cut(s)	795
MspCI	CTTAAG	1 cut(s)	2128
MspI	CCGG	2 cut(s)	30, 1657
MspR9I	CCNGG	3 cut(s)	1055, 1205, 2446
MunI	CAATTG	1 cut(s)	1326
MvaI	CCWGG	3 cut(s)	1055, 1205, 2446
MvnI	CGCG	1 cut(s)	839
MwoI	GCNNNNNNNGC	5 cut(s)	667, 703, 772, 841, 1240
NdeII	GATC	5 cut(s)	1027, 1176, 1465, 1959, 2061
NlaIV	GGNNCC	2 cut(s)	530, 1655
NmeAIII	GCCGAG	2 cut(s)	22, 1098
NmuCI	GTSAC	5 cut(s)	994, 1207, 1627, 2047, 2371
NsiI	ATGCAT	2 cut(s)	1497, 1650
NspI	RCATGY	3 cut(s)	1495, 1915, 2259
NspV	TTCGAA	1 cut(s)	25
PaeR7I	CTCGAG	1 cut(s)	875
PciI	ACATGT	1 cut(s)	1911
PdmI	GAANNNNTTC	2 cut(s)	2123, 2233
PfeI	GAWTC	3 cut(s)	209, 2338, 2344
PflFI	GACNNNGTC	1 cut(s)	1205
PflMI	CCANNNNNTGG	1 cut(s)	802
PkrI	GCNGC	2 cut(s)	40, 1891
PleI	GAGTC	3 cut(s)	76, 2035, 2425
PpsI	GAGTC	3 cut(s)	76, 2035, 2425
PpuMI	RGGWCCY	1 cut(s)	951
PscI	ACATGT	1 cut(s)	1911
PshAI	GACNNNNGTC	1 cut(s)	2045
PshBI	ATTAAT	1 cut(s)	1740
PsiI	TTATAA	2 cut(s)	1002, 1215
Psp124BI	GAGCTC	1 cut(s)	114
Psp1406I	AACGTT	1 cut(s)	1792
Psp5II	RGGWCCY	1 cut(s)	951
Psp6I	CCWGG	3 cut(s)	1053, 1203, 2444
PspGI	CCWGG	3 cut(s)	1053, 1203, 2444
PspN4I	GGNNCC	2 cut(s)	530, 1655
PspPI	GGNCC	4 cut(s)	528, 757, 951, 2442
PspPPI	RGGWCCY	1 cut(s)	951
PspXI	VCTCGAGB	1 cut(s)	875
PsyI	GACNNNGTC	1 cut(s)	1205
RsaI	GTAC	3 cut(s)	92, 686, 1332
RsaNI	GTAC	3 cut(s)	91, 685, 1331
RseI	CAYNNNNRTG	3 cut(s)	1248, 1500, 1935
SacI	GAGCTC	1 cut(s)	114
SalI	GTCGAC	1 cut(s)	1449
SatI	GCNGC	2 cut(s)	39, 1890
Sau3AI	GATC	5 cut(s)	1027, 1176, 1465, 1959, 2061
Sau96I	GGNCC	4 cut(s)	528, 757, 951, 2442
SchI	GAGTC	3 cut(s)	77, 2035, 2425
ScrFI	CCNGG	3 cut(s)	1055, 1205, 2446
SduI	GDGCHC	3 cut(s)	114, 876, 1090
SexAI	ACCWGGT	1 cut(s)	1203
Sfr274I	CTCGAG	1 cut(s)	875
SfuI	TTCGAA	1 cut(s)	25
SinI	GGWCC	3 cut(s)	528, 951, 2442
SlaI	CTCGAG	1 cut(s)	875
SmiMI	CAYNNNNRTG	3 cut(s)	1248, 1500, 1935
SmlI	CTYRAG	5 cut(s)	139, 148, 698, 875, 2128
SmoI	CTYRAG	5 cut(s)	139, 148, 698, 875, 2128
SsiI	CCGC	4 cut(s)	661, 766, 821, 869
SspMI	CTAG	2 cut(s)	735, 2390
SstI	GAGCTC	1 cut(s)	114
StyD4I	CCNGG	3 cut(s)	1053, 1203, 2444
StyI	CCWWGG	2 cut(s)	727, 954
TaaI	ACNGT	4 cut(s)	1007, 1114, 1522, 1852
TaiI	ACGT	2 cut(s)	1795, 2265
TaqI	TCGA	7 cut(s)	25, 250, 817, 876, 1450, 2193, 2323
TatI	WGTACW	2 cut(s)	684, 1330
TfiI	GAWTC	3 cut(s)	209, 2338, 2344
TscAI	CASTG	4 cut(s)	662, 1432, 1969, 2075
TseFI	GTSAC	5 cut(s)	994, 1207, 1627, 2047, 2371
TseI	GCWGC	2 cut(s)	38, 1889
Tsp45I	GTSAC	5 cut(s)	994, 1207, 1627, 2047, 2371
TspDTI	ATGAA	9 cut(s)	483, 630, 980, 1080, 1326, 1604, 1786, 1847, 2027
TspGWI	ACGGA	1 cut(s)	2443
TspRI	CASTG	4 cut(s)	662, 1432, 1969, 2075
Tth111I	GACNNNGTC	1 cut(s)	1205
Van91I	CCANNNNNTGG	1 cut(s)	802
Vha464I	CTTAAG	1 cut(s)	2128
VpaK11BI	GGWCC	3 cut(s)	528, 951, 2442
VspI	ATTAAT	1 cut(s)	1740
XagI	CCTNNNNNAGG	2 cut(s)	958, 1130
XapI	RAATTY	3 cut(s)	403, 433, 1268
XceI	RCATGY	3 cut(s)	1495, 1915, 2259
XhoI	CTCGAG	1 cut(s)	875
XmiI	GTMKAC	1 cut(s)	1450
XmnI	GAANNNNTTC	2 cut(s)	2123, 2233
XspI	CTAG	2 cut(s)	735, 2390
Zsp2I	ATGCAT	2 cut(s)	1497, 1650

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.