Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003496

Physical Location & Seq

Genomic Coordinates Reverse (-)

37830774 .. 37833038

Gene Structure 2265 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1289200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1035 bp

ATGGGTGTGGATTACTACAAGCTTCTGCAGGTGGACAAGAACGCCAGCGACGACGATTTGAAGAAAGCTTACAGAAAGCTCGCCATGAAGTGGCACCCTGATAAGAACCCCACCAACAAGAAGGAAGCCGAGTCCAAGTTCAAACAGATCTCTGAGGCCTACGAGGTTCTGAGTGATCCCCAGAAAAGAGCAATTTATGATCAATACGGAGAAGAAGGCCTGAAAGGCCAAGTGCCGCCGCCGGATGCCGGTGGGCCTGGCGGTGGAGCTACCTTCTTCCAAACTGGGGACGGTCCAAATGTGTTTAGATTCAACCCCAGAAACGCCGACGACATTTTTGCTGATTTCTTTGGGTTCTCGAGCCCCATGGGTGGAATGGGAGGCGGAATGGGCGGTGGCGGCATGAGGGGTGGAAGGTCTTTTGGTGGAATGTTTGGGGAGGACGTGTTCAGTTCATTCGGAGAAGGCCGGCCCATGAGTCATGCGCCACGAAAAGCTCCACCTATAGAGAAGAGGTTGCCTTGCAGCCTTGAGGAGCTGTACAAGGGGACTACCAAGAAGATGAAGATTTCGAGGGAAATCGCAGATGCAAGCGGGAAGACCATGCAAGTGGAGGAAATACTAACCATAGAAATCAAGCCTGGCTGGAAAAAGGGAACCAAAATCACCTTCCCGGAGAAAGGGAACGAGCAAGGAAACATAACTCCTGCGGATCTTGTGTTCATAATCGACGAGAAACCACACAGCACATTCACAAGGGATGGGAATGATCTTATTGTCACGCAGAGGATTACTCTAGCAGAAGCTCTAACAGGTTACACTGTCCGCCTGACCACCCTAGATGGAAGGAGCCTCACCATCCCCATCAACAATGTGATTAGTCCAGACTATGAGGAGGTAGTCCCTAGGGAGGGCATGCCCATACCCAAAGACCCCTCCAAGAAGGGTAACCTCAGAATCAAATTCAACATCAAGTTTCCGACAAGATTGAATGCCGAACAGAAGGCCGGAATTAAGAAATGCTTGCAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001578 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005929 GO:0005930 GO:0006996 GO:0007010 GO:0007017 GO:0008150 GO:0009987 GO:0016043 GO:0022607 GO:0030030 GO:0030031 GO:0031514 GO:0032838 GO:0035082 GO:0036126 GO:0042995 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044441 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044782 GO:0060271 GO:0070925 GO:0071840 GO:0097014 GO:0097223 GO:0097224 GO:0097729 GO:0099568 GO:0120025 GO:0120031 GO:0120036 GO:0120038 GO:1904158

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DnaJ DnaJ_C

Protein Analysis

345

Amino Acids

37.86

Weight (kDa)

9.01

Isoelectric Point (pI)

39.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DnaJ	PF00226	4 - 67	1.8e-28	DnaJ domain
DnaJ_C	PF01556	169 - 327	5.8e-41	DnaJ C terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003496)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20560 AT4G28480 AT4G28480
fragaria_vesca	FvH4_3g08630 FvH4_6g26690
malus_domestica	MD05G1310300.v1.1 MD10G1289200.v1.1
prunus_persica	Prupe.4G052500_v2.0.a1
pyrus_communis	pycom05g28670 pycom10g24180
rosa_chinensis	RchiOBHm_Chr2g0127101 RchiOBHm_Chr5g0009211
rosa_laevigata	RLG00000018979 RLG00000031634 RLG00000031638 RLG00000031640
rosa_multiflora	Rmu_sc0006233.1_g000031 Rmu_sc0008093.1_g000002
rosa_roxburghii	Rroxscaffold_1G00066850 Rroxscaffold_1G00067550 Rroxscaffold_2G00115770 Rroxscaffold_2G00116220
rosa_rugosa	Rorug02G0277300 Rorug02G0277400 Rorug04G0441500
rosa_samantha	Rh2AG327900 Rh2BG337900 Rh2CG315000 Rh2DG355000 Rh5AG071300 Rh5BG067800 Rh5CG080100 Rh5DG067800
rosa_wichuraiana	Rw2G026600 Rw5G006660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	19
Acc36I	ACCTGC	1 cut(s)	19
AccB1I	GGYRCC	1 cut(s)	93
AccB7I	CCANNNNNTGG	1 cut(s)	90
AciI	CCGC	9 cut(s)	236, 239, 261, 384, 393, 399, 594, 710, 826
AclWI	GGATC	2 cut(s)	170, 720
AcsI	RAATTY	1 cut(s)	962
AfaI	GTAC	1 cut(s)	542
AfiI	CCNNNNNNNGG	8 cut(s)	90, 248, 263, 286, 371, 680, 910, 911
AflIII	ACRYGT	1 cut(s)	444
AgsI	TTSAA	5 cut(s)	61, 142, 313, 967, 991
AjiI	CACGTC	1 cut(s)	445
AjnI	CCWGG	2 cut(s)	256, 640
AjuI	GAANNNNNNNTTGG	2 cut(s)	653, 685
AloI	GAACNNNNNNTCC	4 cut(s)	431, 463, 704, 736
AluBI	AGCT	7 cut(s)	22, 68, 79, 269, 497, 538, 806
AluI	AGCT	7 cut(s)	22, 68, 79, 269, 497, 538, 806
AlwI	GGATC	2 cut(s)	170, 720
Ama87I	CYCGRG	1 cut(s)	358
AoxI	GGCC	7 cut(s)	156, 217, 226, 254, 466, 470, 1005
ApeKI	GCWGC	1 cut(s)	525
ApoI	RAATTY	1 cut(s)	962
ArsI	GACNNNNNNTTYG	2 cut(s)	320, 352
AspA2I	CCTAGG	1 cut(s)	905
AspLEI	GCGC	1 cut(s)	487
AspS9I	GGNCC	3 cut(s)	254, 293, 471
AsuC2I	CCSGG	1 cut(s)	674
AsuHPI	GGTGA	2 cut(s)	658, 847
AvaI	CYCGRG	1 cut(s)	358
AvaII	GGWCC	1 cut(s)	293
AvrII	CCTAGG	1 cut(s)	905
BanI	GGYRCC	1 cut(s)	93
BanII	GRGCYC	1 cut(s)	365
BarI	GAAGNNNNNNTAC	2 cut(s)	53, 85
BbsI	GAAGAC	1 cut(s)	605
BbvI	GCAGC	1 cut(s)	537
BccI	CCATC	4 cut(s)	755, 836, 866, 872
BcgI	CGANNNNNNTGC	2 cut(s)	320, 354
BciT130I	CCWGG	2 cut(s)	258, 642
BclI	TGATCA	1 cut(s)	199
BcnI	CCSGG	1 cut(s)	674
BfaI	CTAG	3 cut(s)	797, 839, 906
BfmI	CTRYAG	2 cut(s)	26, 504
BfuAI	ACCTGC	1 cut(s)	19
BglI	GCCNNNNNGGC	1 cut(s)	225
BglII	AGATCT	1 cut(s)	147
BisI	GCNGC	4 cut(s)	236, 239, 400, 526
BlnI	CCTAGG	1 cut(s)	905
BlsI	GCNGC	4 cut(s)	237, 240, 401, 527
Bme1390I	CCNGG	3 cut(s)	258, 642, 674
Bme18I	GGWCC	1 cut(s)	293
BmeT110I	CYCGRG	1 cut(s)	358
BmgBI	CACGTC	1 cut(s)	445
BmgT120I	GGNCC	3 cut(s)	254, 293, 471
BmiI	GGNNCC	3 cut(s)	95, 658, 851
BmrFI	CCNGG	3 cut(s)	258, 642, 674
BmrI	ACTGGG	1 cut(s)	294
BmsI	GCATC	2 cut(s)	235, 577
BmuI	ACTGGG	1 cut(s)	294
BpiI	GAAGAC	1 cut(s)	605
BplI	GAGNNNNNCTC	2 cut(s)	778, 810
BpuEI	CTTGAG	1 cut(s)	551
BpuMI	CCSGG	1 cut(s)	674
BsaJI	CCNNGG	2 cut(s)	366, 905
BsaXI	ACNNNNNCTCC	2 cut(s)	431, 461
Bsc4I	CCNNNNNNNGG	8 cut(s)	90, 248, 263, 286, 371, 680, 910, 911
Bse118I	RCCGGY	2 cut(s)	248, 468
Bse1I	ACTGG	1 cut(s)	289
BseBI	CCWGG	2 cut(s)	258, 642
BseDI	CCNNGG	2 cut(s)	366, 905
BseGI	GGATG	3 cut(s)	250, 766, 858
BseLI	CCNNNNNNNGG	8 cut(s)	90, 248, 263, 286, 371, 680, 910, 911
BseMII	CTCAG	3 cut(s)	144, 161, 967
BseNI	ACTGG	1 cut(s)	289
BseRI	GAGGAG	2 cut(s)	548, 908
BseXI	GCAGC	1 cut(s)	537
BshFI	GGCC	7 cut(s)	158, 219, 228, 256, 468, 472, 1007
BshNI	GGYRCC	1 cut(s)	93
BsiHKCI	CYCGRG	1 cut(s)	358
BsiSI	CCGG	5 cut(s)	242, 249, 469, 674, 1008
BslFI	GGGAC	3 cut(s)	302, 562, 887
BslI	CCNNNNNNNGG	8 cut(s)	90, 248, 263, 286, 371, 680, 910, 911
BsmFI	GGGAC	3 cut(s)	302, 562, 887
BsmI	GAATGC	1 cut(s)	997
BsnI	GGCC	7 cut(s)	158, 219, 228, 256, 468, 472, 1007
BsoBI	CYCGRG	1 cut(s)	358
Bsp1286I	GDGCHC	1 cut(s)	365
Bsp1407I	TGTACA	1 cut(s)	540
Bsp143I	GATC	5 cut(s)	147, 175, 199, 712, 769
Bsp19I	CCATGG	1 cut(s)	366
BspACI	CCGC	9 cut(s)	236, 239, 261, 384, 393, 399, 594, 710, 826
BspANI	GGCC	7 cut(s)	158, 219, 228, 256, 468, 472, 1007
BspCNI	CTCAG	3 cut(s)	145, 162, 966
BspLI	GGNNCC	3 cut(s)	95, 658, 851
BspMAI	CTGCAG	1 cut(s)	30
BspMI	ACCTGC	1 cut(s)	19
BspPI	GGATC	2 cut(s)	170, 720
BspT107I	GGYRCC	1 cut(s)	93
BsrFI	RCCGGY	2 cut(s)	248, 468
BsrGI	TGTACA	1 cut(s)	540
BsrI	ACTGG	1 cut(s)	289
BssAI	RCCGGY	2 cut(s)	248, 468
BssECI	CCNNGG	2 cut(s)	366, 905
BssMI	GATC	5 cut(s)	147, 175, 199, 712, 769
BssT1I	CCWWGG	2 cut(s)	366, 905
Bst2UI	CCWGG	2 cut(s)	258, 642
Bst4CI	ACNGT	2 cut(s)	293, 823
Bst6I	CTCTTC	1 cut(s)	506
BstAUI	TGTACA	1 cut(s)	540
BstC8I	GCNNGC	7 cut(s)	46, 81, 470, 592, 917, 1025, 1029
BstDEI	CTNAG	3 cut(s)	153, 170, 953
BstDSI	CCRYGG	1 cut(s)	366
BstEII	GGTNACC	1 cut(s)	947
BstF5I	GGATG	3 cut(s)	250, 766, 858
BstHHI	GCGC	1 cut(s)	487
BstKTI	GATC	5 cut(s)	150, 178, 202, 715, 772
BstMBI	GATC	5 cut(s)	147, 175, 199, 712, 769
BstMWI	GCNNNNNNNGC	3 cut(s)	225, 390, 399
BstNI	CCWGG	2 cut(s)	258, 642
BstNSI	RCATGY	1 cut(s)	919
BstPI	GGTNACC	1 cut(s)	947
BstSCI	CCNGG	3 cut(s)	256, 640, 672
BstSFI	CTRYAG	2 cut(s)	26, 504
BstV1I	GCAGC	1 cut(s)	537
BstV2I	GAAGAC	1 cut(s)	605
BstX2I	RGATCY	2 cut(s)	147, 712
BstXI	CCANNNNNNTGG	1 cut(s)	610
BstYI	RGATCY	2 cut(s)	147, 712
BsuRI	GGCC	7 cut(s)	158, 219, 228, 256, 468, 472, 1007
BtgI	CCRYGG	1 cut(s)	366
BtrI	CACGTC	1 cut(s)	445
BtsCI	GGATG	3 cut(s)	250, 766, 858
BtsIMutI	CAGTG	1 cut(s)	819
BveI	ACCTGC	1 cut(s)	19
Cac8I	GCNNGC	7 cut(s)	46, 81, 470, 592, 917, 1025, 1029
CfoI	GCGC	1 cut(s)	487
Cfr10I	RCCGGY	2 cut(s)	248, 468
Cfr13I	GGNCC	3 cut(s)	254, 293, 471
Csp6I	GTAC	1 cut(s)	541
CviAII	CATG	7 cut(s)	85, 367, 403, 475, 482, 604, 916
CviQI	GTAC	1 cut(s)	541
DdeI	CTNAG	3 cut(s)	153, 170, 953
DpnI	GATC	5 cut(s)	149, 177, 201, 714, 771
DpnII	GATC	5 cut(s)	147, 175, 199, 712, 769
Eam1104I	CTCTTC	1 cut(s)	506
EarI	CTCTTC	1 cut(s)	506
EciI	GGCGGA	2 cut(s)	399, 815
Eco130I	CCWWGG	2 cut(s)	366, 905
Eco147I	AGGCCT	2 cut(s)	158, 219
Eco24I	GRGCYC	1 cut(s)	365
Eco47I	GGWCC	1 cut(s)	293
Eco88I	CYCGRG	1 cut(s)	358
Eco91I	GGTNACC	1 cut(s)	947
EcoO65I	GGTNACC	1 cut(s)	947
EcoRII	CCWGG	2 cut(s)	256, 640
EcoT14I	CCWWGG	2 cut(s)	366, 905
EcoT38I	GRGCYC	1 cut(s)	365
ErhI	CCWWGG	2 cut(s)	366, 905
FaeI	CATG	7 cut(s)	88, 370, 406, 478, 485, 607, 919
FalI	AAGNNNNNCTT	1 cut(s)	1007
FaqI	GGGAC	3 cut(s)	302, 562, 887
FatI	CATG	7 cut(s)	84, 366, 402, 474, 481, 603, 915
FauI	CCCGC	1 cut(s)	587
FbaI	TGATCA	1 cut(s)	199
Fnu4HI	GCNGC	4 cut(s)	236, 239, 400, 526
FokI	GGATG	3 cut(s)	257, 773, 845
FriOI	GRGCYC	1 cut(s)	365
FseI	GGCCGGCC	1 cut(s)	472
Fsp4HI	GCNGC	4 cut(s)	236, 239, 400, 526
FspBI	CTAG	3 cut(s)	797, 839, 906
GlaI	GCGC	1 cut(s)	486
GluI	GCNGC	4 cut(s)	236, 239, 400, 526
HaeIII	GGCC	7 cut(s)	158, 219, 228, 256, 468, 472, 1007
HapII	CCGG	5 cut(s)	242, 249, 469, 674, 1008
HhaI	GCGC	1 cut(s)	487
Hin1II	CATG	7 cut(s)	88, 370, 406, 478, 485, 607, 919
Hin6I	GCGC	1 cut(s)	485
HinP1I	GCGC	1 cut(s)	485
HindIII	AAGCTT	2 cut(s)	20, 66
HinfI	GANTC	4 cut(s)	131, 309, 478, 957
HpaII	CCGG	5 cut(s)	242, 249, 469, 674, 1008
HphI	GGTGA	2 cut(s)	658, 847
Hpy166II	GTNNAC	1 cut(s)	34
Hpy188I	TCNGA	5 cut(s)	154, 171, 461, 956, 981
Hpy188III	TCNNGA	2 cut(s)	358, 884
Hpy8I	GTNNAC	1 cut(s)	34
Hpy99I	CGWCG	4 cut(s)	53, 56, 332, 734
HpyAV	CCTTC	9 cut(s)	115, 209, 283, 408, 458, 679, 840, 937, 997
HpyCH4III	ACNGT	2 cut(s)	293, 823
HpyCH4IV	ACGT	1 cut(s)	444
HpyCH4V	TGCA	5 cut(s)	28, 525, 590, 607, 1027
HpyF10VI	GCNNNNNNNGC	3 cut(s)	225, 390, 399
HpyF3I	CTNAG	3 cut(s)	153, 170, 953
HpySE526I	ACGT	1 cut(s)	444
Hsp92II	CATG	7 cut(s)	88, 370, 406, 478, 485, 607, 919
HspAI	GCGC	1 cut(s)	485
KroI	GCCGGC	1 cut(s)	468
KroNI	GCCGGC	1 cut(s)	470
Ksp22I	TGATCA	1 cut(s)	199
Kzo9I	GATC	5 cut(s)	147, 175, 199, 712, 769
LmnI	GCTCC	4 cut(s)	266, 502, 535, 849
Lsp1109I	GCAGC	1 cut(s)	537
LweI	GCATC	2 cut(s)	235, 577
MaeI	CTAG	3 cut(s)	797, 839, 906
MaeII	ACGT	1 cut(s)	444
MaeIII	GTNAC	3 cut(s)	778, 815, 947
MalI	GATC	5 cut(s)	149, 177, 201, 714, 771
MboI	GATC	5 cut(s)	147, 175, 199, 712, 769
MboII	GAAGA	7 cut(s)	73, 224, 268, 523, 571, 577, 610
MflI	RGATCY	2 cut(s)	147, 712
MhlI	GDGCHC	1 cut(s)	365
MluCI	AATT	3 cut(s)	192, 962, 1011
MlyI	GAGTC	2 cut(s)	140, 487
MmeI	TCCRAC	1 cut(s)	1004
MroNI	GCCGGC	1 cut(s)	468
MseI	TTAA	1 cut(s)	1014
MslI	CAYNNNNRTG	1 cut(s)	608
MspI	CCGG	5 cut(s)	242, 249, 469, 674, 1008
MspR9I	CCNGG	3 cut(s)	258, 642, 674
Mva1269I	GAATGC	1 cut(s)	997
MvaI	CCWGG	2 cut(s)	258, 642
MwoI	GCNNNNNNNGC	3 cut(s)	225, 390, 399
NaeI	GCCGGC	1 cut(s)	470
NciI	CCSGG	1 cut(s)	674
NcoI	CCATGG	1 cut(s)	366
NdeII	GATC	5 cut(s)	147, 175, 199, 712, 769
NgoMIV	GCCGGC	1 cut(s)	468
NlaIII	CATG	7 cut(s)	88, 370, 406, 478, 485, 607, 919
NlaIV	GGNNCC	3 cut(s)	95, 658, 851
NmeAIII	GCCGAG	1 cut(s)	154
NmuCI	GTSAC	1 cut(s)	778
NspI	RCATGY	1 cut(s)	919
PaeI	GCATGC	1 cut(s)	919
PaeR7I	CTCGAG	1 cut(s)	358
PaqCI	CACCTGC	1 cut(s)	19
PceI	AGGCCT	2 cut(s)	158, 219
PcsI	WCGNNNNNNNCGW	1 cut(s)	48
PctI	GAATGC	1 cut(s)	997
PdiI	GCCGGC	1 cut(s)	470
PfeI	GAWTC	2 cut(s)	309, 957
PflMI	CCANNNNNTGG	1 cut(s)	90
PfoI	TCCNGGA	1 cut(s)	672
PkrI	GCNGC	4 cut(s)	237, 240, 401, 527
PleI	GAGTC	2 cut(s)	139, 486
PpsI	GAGTC	2 cut(s)	139, 486
Psp6I	CCWGG	2 cut(s)	256, 640
PspEI	GGTNACC	1 cut(s)	947
PspGI	CCWGG	2 cut(s)	256, 640
PspN4I	GGNNCC	3 cut(s)	95, 658, 851
PspPI	GGNCC	3 cut(s)	254, 293, 471
PstI	CTGCAG	1 cut(s)	30
PsuI	RGATCY	2 cut(s)	147, 712
RigI	GGCCGGCC	1 cut(s)	472
RsaI	GTAC	1 cut(s)	542
RsaNI	GTAC	1 cut(s)	541
RseI	CAYNNNNRTG	1 cut(s)	608
SaqAI	TTAA	1 cut(s)	1014
SatI	GCNGC	4 cut(s)	236, 239, 400, 526
Sau3AI	GATC	5 cut(s)	147, 175, 199, 712, 769
Sau96I	GGNCC	3 cut(s)	254, 293, 471
SchI	GAGTC	2 cut(s)	140, 487
ScrFI	CCNGG	3 cut(s)	258, 642, 674
SduI	GDGCHC	1 cut(s)	365
SfaNI	GCATC	2 cut(s)	235, 577
SfcI	CTRYAG	2 cut(s)	26, 504
SfiI	GGCCNNNNNGGCC	1 cut(s)	225
Sfr274I	CTCGAG	1 cut(s)	358
SinI	GGWCC	1 cut(s)	293
SlaI	CTCGAG	1 cut(s)	358
SmiMI	CAYNNNNRTG	1 cut(s)	608
SmlI	CTYRAG	2 cut(s)	358, 530
SmoI	CTYRAG	2 cut(s)	358, 530
SphI	GCATGC	1 cut(s)	919
Sse9I	AATT	3 cut(s)	192, 962, 1011
SseBI	AGGCCT	2 cut(s)	158, 219
SsiI	CCGC	9 cut(s)	236, 239, 261, 384, 393, 399, 594, 710, 826
SspMI	CTAG	3 cut(s)	797, 839, 906
StuI	AGGCCT	2 cut(s)	158, 219
StyD4I	CCNGG	3 cut(s)	256, 640, 672
StyI	CCWWGG	2 cut(s)	366, 905
TaaI	ACNGT	2 cut(s)	293, 823
TaiI	ACGT	1 cut(s)	447
TaqI	TCGA	3 cut(s)	359, 572, 729
TasI	AATT	3 cut(s)	192, 962, 1011
TatI	WGTACW	1 cut(s)	540
TauI	GCSGC	3 cut(s)	238, 241, 402
TfiI	GAWTC	2 cut(s)	309, 957
Tru1I	TTAA	1 cut(s)	1014
Tru9I	TTAA	1 cut(s)	1014
TscAI	CASTG	1 cut(s)	826
TseFI	GTSAC	1 cut(s)	778
TseI	GCWGC	1 cut(s)	525
Tsp45I	GTSAC	1 cut(s)	778
TspDTI	ATGAA	4 cut(s)	101, 444, 578, 712
TspGWI	ACGGA	1 cut(s)	222
TspRI	CASTG	1 cut(s)	826
Van91I	CCANNNNNTGG	1 cut(s)	90
VpaK11BI	GGWCC	1 cut(s)	293
XapI	RAATTY	1 cut(s)	962
XceI	RCATGY	1 cut(s)	919
XcmI	CCANNNNNNNNNTGG	1 cut(s)	373
XhoI	CTCGAG	1 cut(s)	358
XmaJI	CCTAGG	1 cut(s)	905
XspI	CTAG	3 cut(s)	797, 839, 906

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.