Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003373

Physical Location & Seq

Genomic Coordinates Reverse (-)

39112395 .. 39118408

Gene Structure 6014 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1304800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3093 bp

ATGGGCGAGGAGGGTGCGCTGCCGGACCATCTGCGGTGCGGCCGGACCGACGGCCGGAAATGGCGGTGCAAGCGGAGAGTCATGGACGACATGAAGCTCTGCGAGATTCACTATCTCCAAGGCCGTCACCGTCAGTTCAGAGAGAAAGTCCCCGAGTCACTCAAGCTTCAGAGGACGCCCAAAAACGCTGGGGACAAAGATCAAAACGGCAGCGGAAACGGCGGTGGAGTCAAAATTAGGGCACGGAAAGTGGAGAATTTGGTGAAGTTGCTGAAGCGGAAGCGATCCGACGAGGCGGTGAAGAATTGCAAGAAGAAGAAGCGGAAGGTGAAGTTGAAGAAGAGCGAGTTGAATTTGGACCTCATACGGATGGTGCTGAGGAGGGAGGTCGAGAAGCGGAACCAGACGACTACGAAGAAGAAGAATGTTGTGGAGGAGGAGAGTGAGGATGGTGATGAAGACGACGATGACGGCGGCGGTGATCTCACCAGAGACTTGCCCAATGGCCGAATGGCAATCTCTTCATCTTCCTCGCAGTCGCCGAGGCTTCGGTCCGGCAATGCAGGTTCCAATTCGTCTTCTGATGGGAAGGTCGGGGCTGATTTGCCTCCGGTTGCTACGCGGAGGAGGTGCTTTCGGTCAAAGAACATCGAGCCAATTCCTGCTGGCACATTGCAGGTTCTGCCATATAATGATGTGGGGAAGTTGAGGAAAGGTAAGAGGAGAAGGTGCCATTGGTGTCGAAAAAGAGGGAGCGGTGTTTCTTCTGCTCTAATTAAGTGTTCGAGTTGCCAGAAGCACTTCTTTTGCTTGAATTGCGTCAAAGAAAGGTACTTTGATACACAAGATGAAGTCAAAATGGCATGCCCAGTCTGTCGAGGAACTTGCCCCTGTAAGGAATGCTCTGAAAATCAATCAAAAGATGCGGAAAGTAAGGATTATTTGGGGGTTAAGAATAAAGTTGAAGTAATACTACAGTTCCATTATCTGATTTGTATGCTTCTTCCCGTGCTAAAACAAATAAACCAAGATCAGAAAGTTGAGCTAGAAGCAGAGGCAAAAATGAGAGGGGAAGAGCTATCTGAAGTTCATATCAAGCAGGCTGAATATAGCTGCAACGAACAACACTGCTGCAATAAATGCAAAGCTTCAATAGTGGATCTCCATAGAAGCTGCCCAAATTGTTCCTATAACCTTTGTTTAAGTTGCTGTCGAGATCTTTTAAGTGGGAGCCTTTTTGGTGGTATAAACACAACTCTCATAAAGCAGACTAACAAAAAGAAAACTTGTGTCTCTCGTAAGGGGCAACTTGTAAAGAAGCCAATAACCACCCATAAGCAAAGTTTCCGCAGTTTGTACCCTTCTTCTGCATCAGTACCTAGCTTGAAATCTTGTAATGCTGTTAATGGTATATCTTGCCCACCTAAGGAGCTTGGAGGTTGTGGTGACAGCCTCCTTGATTTGAGATGTGTTTTTCCATTATGTTGGATCAAAGACCTGGAAGTAAGTGCAGAAGAAATAGTTTGCAGCTATGAATTTCCAGAAACTGCTGACATGTCTTTGTGCTGTCCACTATGTTTGGGCGTGGACCAGAAAACTGATGGGAATCGACAGTTGCAAGAAGCTTCAGTGAGAGAGAACTCAAACGATAACTACTTATACTACCCCACGCTACTGGGCACAAATGGTGATAATGTTGAGCACTTTCAGAAGCACTGGAGTAAAGGTCATCCTGTAATAGTCCGAGACGTACTTCAAACTACATCAGATTTGACTTGGGATCCAGTGTCCATGTTCTGCACTTATCTTGAGAGGAGCATTGCTAGATATGAAAATAACACAAATTCAAATGAAGCTATCCACTGCTTGGATTGGTGCGAGGTGGAACTTGGAATCAGGCAGTACTTCATGGGTTCATTAAGGGGGCAGGCCCAACGAAATGTATGGAATGAGACACTGAAGTTGAAGGGTTGGCTTTCTTCGCAGTTGTTTCAGGAACAGTTTCCAGTTCATTATGCTGAAGTAATACGAGCTCTACCACTTCAAGAATACATGAATCCCACGTCTGGTCTTTTAAATCTTGCTGCAAGGATGCCACAAGAAATCCCAAAACCTGATCTAGGTCCCTGTGTTTATATATCATACGGCTGCACCGAGCAACTTGTACAAGCTAATGCAGTGATAAAGCTATGTTATGACTCATATGATGTGGTTAACATTTTGGCACATACATCAGATGTCCCTATCTCTGATGAACAAGTTTCAAAAATAAGAAAGTTACTGAAGAAGCACAAGGCTCAATATCAGAGGGAGGGTTCTAGGGTTACTTGTGAACAATTTGTGGCAAAAAAAGACAATGGAGAATCATTATTGTTTAGTGAAACCATGAAAGAAGCAGGGCTACATAATGTGATCGGAGAGGAGATGCATTTACGCAAAAGAATTGCTAGAGAGTCTTGCTTCTCCAGGCATGAAGCATGCACAGATGCTGAAACATCTGATTCTGATACTGATTCTGAAGCCACACTAAGTAGCTCTGGGAGACTTCATGATGCTGAAACATCTAAAGATACAAGGTGTGAGGTTCTGGTCGACAGCTGCAATAGCTATGAAAAGCAAACCTTGGACAAGTCTTGTGGTGCCCAATGGGATGTGTTTCGCAGACAAGATGTCCCAAAACTTATAGAATATCTCAGAAGGCACTCTAATGAATTCACTCGTAAATTTGACTTTCATAAGCATGTTGTTCATCCCATTCTTGATCAGAGTTTCTTTCTGGACTCAAGTCACAAATTAAGGCTCAAGGAGGAATTTAAAATTGAGCCTTGGACTTTTGAGCAACACATAGGAGAAGCTGTTATCATACCTGCTGGATGCCCATATCAGATTAGGAATTCTAAGTCTTGTGTACATGTGGTGCTGGACTTTGTATCACCCGAAAATGTTGCCGAGTGCATCCAGTTAACTGATGAAGTCCGTCTGCTTCCAGCAGACCATAAAGCAAAAGTTGACAAGCTAGAGGTGAAGAGAATGGCCCTTAATAGTATTAGTTCAGCAATCAAAGAAATACGTGAGCTTACTTGTGCAATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008214 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031326 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0033169 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04714 map04714

Pfam Domains

Protein Families

JmjC WRC

Protein Analysis

1031

Amino Acids

116.82

Weight (kDa)

8.57

Isoelectric Point (pI)

56.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRC	PF08879	8 - 49	3.7e-16	WRC
JmjC	PF02373	873 - 976	2e-11	JmjC domain, hydroxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003373)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21430
fragaria_vesca	FvH4_3g03760 FvH4_3g03760 FvH4_3g03760 FvH4_3g03760 FvH4_3g03760 FvH4_3g03760
malus_domestica	MD05G1326700.v1.1 MD10G1304800.v1.1
prunus_persica	Prupe.4G034800_v2.0.a1 Prupe.4G034800_v2.0.a1 Prupe.4G034800_v2.0.a1 Prupe.4G034800_v2.0.a1 Prupe.4G034800_v2.0.a1 Prupe.4G034800_v2.0.a1 Prupe.4G034800_v2.0.a1
pyrus_communis	pycom05g30110 pycom10g25720
rosa_chinensis	RchiOBHm_Chr5g0005871
rosa_laevigata	RLG00000016376 RLG00000031332 RLG00000031334 RLG00000031337 RLG00000031345 RLG00000031346 RLG00000031385
rosa_multiflora	Rmu_sc0002694.1_g000004
rosa_roxburghii	Rroxscaffold_1G00070030
rosa_rugosa	Rorug04G0418700 Rorug04G0418800 Rorug04G0418900
rosa_samantha	Rh5AG046200 Rh5BG044900 Rh5CG053800 Rh5DG044700
rosa_wichuraiana	Rw5G004640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	554, 667, 2875
AccB1I	GGYRCC	2 cut(s)	729, 2639
AccB7I	CCANNNNNTGG	1 cut(s)	1867
AccBSI	CCGCTC	1 cut(s)	756
AccI	GTMKAC	1 cut(s)	2592
AccII	CGCG	1 cut(s)	622
AclWI	GGATC	5 cut(s)	279, 1167, 1496, 1775, 1788
AcoI	YGGCCR	3 cut(s)	40, 52, 505
AcsI	RAATTY	8 cut(s)	256, 352, 1535, 1843, 2711, 2723, 2810, 2893
AcuI	CTGAAG	8 cut(s)	152, 293, 1104, 1611, 1979, 2040, 2303, 2538
AcyI	GRCGYC	1 cut(s)	176
AfaI	GTAC	7 cut(s)	833, 1358, 1377, 1752, 1904, 2166, 2910
AfiI	CCNNNNNNNGG	6 cut(s)	54, 895, 1426, 1867, 2066, 2120
AflIII	ACRYGT	2 cut(s)	1554, 2911
AhdI	GACNNNNNGTC	1 cut(s)	2669
AjiI	CACGTC	1 cut(s)	2064
AjnI	CCWGG	2 cut(s)	1497, 2465
AjuI	GAANNNNNNNTTGG	2 cut(s)	718, 750
Alw21I	GWGCWC	2 cut(s)	1704, 2035
Alw26I	GTCTC	5 cut(s)	486, 1297, 1740, 1946, 2536
AlwI	GGATC	5 cut(s)	279, 1167, 1496, 1775, 1788
AlwNI	CAGNNNCTG	3 cut(s)	682, 1547, 2489
Ama87I	CYCGRG	1 cut(s)	152
AoxI	GGCC	6 cut(s)	40, 52, 121, 505, 1929, 3033
ApeKI	GCWGC	9 cut(s)	19, 210, 1113, 1131, 1173, 1527, 2084, 2148, 2598
ApoI	RAATTY	8 cut(s)	256, 352, 1535, 1843, 2711, 2723, 2810, 2893
Asp700I	GAANNNNTTC	2 cut(s)	800, 2001
AspLEI	GCGC	1 cut(s)	19
AspS9I	GGNCC	8 cut(s)	25, 45, 358, 552, 1588, 1930, 2123, 3034
AvaI	CYCGRG	1 cut(s)	152
AvaII	GGWCC	6 cut(s)	25, 45, 358, 552, 1588, 2123
AxyI	CCTNAGG	1 cut(s)	1425
BaeGI	GKGCMC	3 cut(s)	244, 1682, 2644
BamHI	GGATCC	1 cut(s)	1780
BanI	GGYRCC	2 cut(s)	729, 2639
BanII	GRGCYC	1 cut(s)	2035
BarI	GAAGNNNNNNTAC	4 cut(s)	957, 989, 2385, 2417
BbsI	GAAGAC	2 cut(s)	465, 570
Bbv12I	GWGCWC	2 cut(s)	1704, 2035
BbvCI	CCTCAGC	1 cut(s)	377
BbvI	GCAGC	9 cut(s)	6, 222, 1100, 1118, 1160, 1539, 2071, 2135, 2585
BccI	CCATC	5 cut(s)	36, 364, 443, 578, 1595
BceAI	ACGGC	6 cut(s)	67, 108, 223, 235, 487, 2161
BcgI	CGANNNNNNTGC	5 cut(s)	30, 867, 901, 2927, 2961
BciT130I	CCWGG	2 cut(s)	1499, 2467
BclI	TGATCA	1 cut(s)	2761
BcoDI	GTCTC	5 cut(s)	486, 1297, 1740, 1946, 2536
BfaI	CTAG	7 cut(s)	1046, 1380, 1824, 2120, 2319, 2448, 3017
BfmI	CTRYAG	1 cut(s)	974
BfuAI	ACCTGC	3 cut(s)	554, 667, 2875
BglII	AGATCT	1 cut(s)	1216
BmcAI	AGTACT	1 cut(s)	1904
Bme1390I	CCNGG	2 cut(s)	1499, 2467
Bme18I	GGWCC	6 cut(s)	25, 45, 358, 552, 1588, 2123
BmeRI	GACNNNNNGTC	1 cut(s)	2669
BmeT110I	CYCGRG	1 cut(s)	152
BmgBI	CACGTC	1 cut(s)	2064
BmgT120I	GGNCC	8 cut(s)	25, 45, 358, 552, 1588, 1930, 2123, 3034
BmiI	GGNNCC	7 cut(s)	401, 568, 731, 1232, 1782, 2125, 2641
BmrFI	CCNGG	2 cut(s)	1499, 2467
BmrI	ACTGGG	2 cut(s)	863, 1685
BmsI	GCATC	8 cut(s)	913, 1379, 2082, 2415, 2476, 2542, 2864, 2964
BmuI	ACTGGG	2 cut(s)	863, 1685
BoxI	GACNNNNGTC	1 cut(s)	2784
BpiI	GAAGAC	2 cut(s)	465, 570
BpmI	CTGGAG	2 cut(s)	1738, 2449
Bpu10I	CCTNAGC	1 cut(s)	377
BpuEI	CTTGAG	4 cut(s)	146, 1829, 2767, 2786
BsaAI	YACGTR	1 cut(s)	3071
BsaBI	GATNNNNATC	1 cut(s)	1605
BsaHI	GRCGYC	1 cut(s)	176
BsaJI	CCNNGG	4 cut(s)	118, 542, 2622, 2825
BsaWI	WCCGGW	1 cut(s)	610
BsaXI	ACNNNNNCTCC	4 cut(s)	1711, 1741, 2841, 2871
Bsc4I	CCNNNNNNNGG	6 cut(s)	54, 895, 1426, 1867, 2066, 2120
Bse1I	ACTGG	6 cut(s)	869, 1680, 1721, 1784, 2006, 2959
Bse21I	CCTNAGG	1 cut(s)	1425
Bse3DI	GCAATG	4 cut(s)	565, 671, 1818, 3093
Bse8I	GATNNNNATC	1 cut(s)	1605
BseBI	CCWGG	2 cut(s)	1499, 2467
BseDI	CCNNGG	4 cut(s)	118, 542, 2622, 2825
BseGI	GGATG	8 cut(s)	375, 454, 1729, 2097, 2656, 2749, 2879, 2955
BseJI	GATNNNNATC	1 cut(s)	1605
BseLI	CCNNNNNNNGG	6 cut(s)	54, 895, 1426, 1867, 2066, 2120
BseMI	GCAATG	4 cut(s)	565, 671, 1818, 3093
BseMII	CTCAG	2 cut(s)	368, 2707
BseNI	ACTGG	6 cut(s)	869, 1680, 1721, 1784, 2006, 2959
BseRI	GAGGAG	8 cut(s)	23, 394, 449, 452, 640, 736, 1828, 2435
BseSI	GKGCMC	3 cut(s)	244, 1682, 2644
BseX3I	CGGCCG	2 cut(s)	40, 52
BseXI	GCAGC	9 cut(s)	6, 222, 1100, 1118, 1160, 1539, 2071, 2135, 2585
BseYI	CCCAGC	1 cut(s)	188
BsgI	GTGCAG	3 cut(s)	1530, 1783, 2134
Bsh1236I	CGCG	1 cut(s)	622
Bsh1285I	CGRYCG	2 cut(s)	43, 55
BshFI	GGCC	6 cut(s)	42, 54, 123, 507, 1931, 3035
BshNI	GGYRCC	2 cut(s)	729, 2639
BsiEI	CGRYCG	2 cut(s)	43, 55
BsiHKAI	GWGCWC	2 cut(s)	1704, 2035
BsiHKCI	CYCGRG	1 cut(s)	152
BsiSI	CCGG	5 cut(s)	23, 43, 55, 555, 611
BslFI	GGGAC	5 cut(s)	134, 206, 2109, 2225, 2657
BslI	CCNNNNNNNGG	6 cut(s)	54, 895, 1426, 1867, 2066, 2120
BsmAI	GTCTC	5 cut(s)	486, 1297, 1740, 1946, 2536
BsmBI	CGTCTC	1 cut(s)	1740
BsmFI	GGGAC	5 cut(s)	134, 206, 2109, 2225, 2657
BsmI	GAATGC	1 cut(s)	905
BsnI	GGCC	6 cut(s)	42, 54, 123, 507, 1931, 3035
BsoBI	CYCGRG	1 cut(s)	152
Bsp1286I	GDGCHC	5 cut(s)	244, 1682, 1704, 2035, 2644
Bsp1407I	TGTACA	2 cut(s)	2164, 2908
BspANI	GGCC	6 cut(s)	42, 54, 123, 507, 1931, 3035
BspCNI	CTCAG	2 cut(s)	369, 2706
BspFNI	CGCG	1 cut(s)	622
BspHI	TCATGA	1 cut(s)	2548
BspLI	GGNNCC	7 cut(s)	401, 568, 731, 1232, 1782, 2125, 2641
BspMI	ACCTGC	3 cut(s)	554, 667, 2875
BspPI	GGATC	5 cut(s)	279, 1167, 1496, 1775, 1788
BspQI	GCTCTTC	2 cut(s)	335, 1068
BspT107I	GGYRCC	2 cut(s)	729, 2639
BsrBI	CCGCTC	1 cut(s)	756
BsrDI	GCAATG	4 cut(s)	565, 671, 1818, 3093
BsrGI	TGTACA	2 cut(s)	2164, 2908
BsrI	ACTGG	6 cut(s)	869, 1680, 1721, 1784, 2006, 2959
BssECI	CCNNGG	4 cut(s)	118, 542, 2622, 2825
BssNI	GRCGYC	1 cut(s)	176
BssT1I	CCWWGG	3 cut(s)	118, 2622, 2825
Bst2UI	CCWGG	2 cut(s)	1499, 2467
Bst4CI	ACNGT	4 cut(s)	131, 978, 1614, 2001
Bst6I	CTCTTC	4 cut(s)	335, 526, 1068, 3020
BstACI	GRCGYC	1 cut(s)	176
BstAPI	GCANNNNNTGC	2 cut(s)	682, 1140
BstAUI	TGTACA	2 cut(s)	2164, 2908
BstBAI	YACGTR	1 cut(s)	3071
BstC8I	GCNNGC	6 cut(s)	71, 667, 865, 1101, 1929, 2479
BstDEI	CTNAG	5 cut(s)	377, 1425, 2528, 2693, 2898
BstENI	CCTNNNNNAGG	1 cut(s)	2118
BstF5I	GGATG	8 cut(s)	375, 454, 1729, 2097, 2656, 2749, 2879, 2955
BstFNI	CGCG	1 cut(s)	622
BstHHI	GCGC	1 cut(s)	19
BstMAI	GTCTC	5 cut(s)	486, 1297, 1740, 1946, 2536
BstMCI	CGRYCG	2 cut(s)	43, 55
BstMWI	GCNNNNNNNGC	7 cut(s)	70, 219, 682, 816, 1140, 1981, 2604
BstNI	CCWGG	2 cut(s)	1499, 2467
BstNSI	RCATGY	5 cut(s)	867, 1558, 2481, 2744, 2915
BstPAI	GACNNNNGTC	1 cut(s)	2784
BstSCI	CCNGG	2 cut(s)	1497, 2465
BstSFI	CTRYAG	1 cut(s)	974
BstSLI	GKGCMC	3 cut(s)	244, 1682, 2644
BstUI	CGCG	1 cut(s)	622
BstV1I	GCAGC	9 cut(s)	6, 222, 1100, 1118, 1160, 1539, 2071, 2135, 2585
BstV2I	GAAGAC	2 cut(s)	465, 570
BstX2I	RGATCY	3 cut(s)	1159, 1216, 1780
BstXI	CCANNNNNNTGG	2 cut(s)	1485, 1675
BstYI	RGATCY	3 cut(s)	1159, 1216, 1780
BstZI	CGGCCG	2 cut(s)	40, 52
Bsu36I	CCTNAGG	1 cut(s)	1425
BsuRI	GGCC	6 cut(s)	42, 54, 123, 507, 1931, 3035
BtrI	CACGTC	1 cut(s)	2064
BtsCI	GGATG	8 cut(s)	375, 454, 1729, 2097, 2656, 2749, 2879, 2955
BtsI	GCAGTG	3 cut(s)	1126, 1861, 2184
BtsIMutI	CAGTG	7 cut(s)	1126, 1635, 1714, 1791, 1861, 1955, 2184
BveI	ACCTGC	3 cut(s)	554, 667, 2875
Cac8I	GCNNGC	6 cut(s)	71, 667, 865, 1101, 1929, 2479
CaiI	CAGNNNCTG	3 cut(s)	682, 1547, 2489
CciI	TCATGA	1 cut(s)	2548
CfoI	GCGC	1 cut(s)	19
Cfr13I	GGNCC	8 cut(s)	25, 45, 358, 552, 1588, 1930, 2123, 3034
CpoI	CGGWCCG	2 cut(s)	45, 552
CseI	GACGC	2 cut(s)	184, 808
Csp6I	GTAC	7 cut(s)	832, 1357, 1376, 1751, 1903, 2165, 2909
CspCI	CAANNNNNGTGG	4 cut(s)	1560, 1595, 2617, 2652
CspI	CGGWCCG	2 cut(s)	45, 552
CviQI	GTAC	7 cut(s)	832, 1357, 1376, 1751, 1903, 2165, 2909
DdeI	CTNAG	5 cut(s)	377, 1425, 2528, 2693, 2898
DraI	TTTAAA	2 cut(s)	2076, 2815
DriI	GACNNNNNGTC	1 cut(s)	2669
EaeI	YGGCCR	3 cut(s)	40, 52, 505
EagI	CGGCCG	2 cut(s)	40, 52
Eam1104I	CTCTTC	4 cut(s)	335, 526, 1068, 3020
Eam1105I	GACNNNNNGTC	1 cut(s)	2669
EarI	CTCTTC	4 cut(s)	335, 526, 1068, 3020
Ecl136II	GAGCTC	1 cut(s)	2033
EclXI	CGGCCG	2 cut(s)	40, 52
Eco130I	CCWWGG	3 cut(s)	118, 2622, 2825
Eco24I	GRGCYC	1 cut(s)	2035
Eco47I	GGWCC	6 cut(s)	25, 45, 358, 552, 1588, 2123
Eco52I	CGGCCG	2 cut(s)	40, 52
Eco53kI	GAGCTC	1 cut(s)	2033
Eco57I	CTGAAG	8 cut(s)	152, 293, 1104, 1611, 1979, 2040, 2303, 2538
Eco81I	CCTNAGG	1 cut(s)	1425
Eco88I	CYCGRG	1 cut(s)	152
EcoICRI	GAGCTC	1 cut(s)	2033
EcoNI	CCTNNNNNAGG	1 cut(s)	2118
EcoO109I	RGGNCCY	1 cut(s)	2123
EcoRI	GAATTC	2 cut(s)	2711, 2893
EcoRII	CCWGG	2 cut(s)	1497, 2465
EcoT14I	CCWWGG	3 cut(s)	118, 2622, 2825
EcoT22I	ATGCAT	1 cut(s)	2430
EcoT38I	GRGCYC	1 cut(s)	2035
ErhI	CCWWGG	3 cut(s)	118, 2622, 2825
Esp3I	CGTCTC	1 cut(s)	1740
FalI	AAGNNNNNCTT	4 cut(s)	788, 820, 2606, 2638
FaqI	GGGAC	5 cut(s)	134, 206, 2109, 2225, 2657
FauNDI	CATATG	1 cut(s)	2203
FbaI	TGATCA	1 cut(s)	2761
FblI	GTMKAC	1 cut(s)	2592
FokI	GGATG	8 cut(s)	382, 461, 1716, 2104, 2663, 2736, 2886, 2942
FriOI	GRGCYC	1 cut(s)	2035
FspBI	CTAG	7 cut(s)	1046, 1380, 1824, 2120, 2319, 2448, 3017
GlaI	GCGC	1 cut(s)	18
GsaI	CCCAGC	1 cut(s)	192
GsuI	CTGGAG	2 cut(s)	1738, 2449
HaeIII	GGCC	6 cut(s)	42, 54, 123, 507, 1931, 3035
HapII	CCGG	5 cut(s)	23, 43, 55, 555, 611
HgaI	GACGC	2 cut(s)	184, 808
HhaI	GCGC	1 cut(s)	19
Hin1I	GRCGYC	1 cut(s)	176
Hin6I	GCGC	1 cut(s)	17
HinP1I	GCGC	1 cut(s)	17
HincII	GTYRAC	4 cut(s)	2215, 2593, 2964, 3010
HindII	GTYRAC	4 cut(s)	2215, 2593, 2964, 3010
HindIII	AAGCTT	3 cut(s)	164, 1146, 1623
HpaI	GTTAAC	2 cut(s)	2215, 2964
HpaII	CCGG	5 cut(s)	23, 43, 55, 555, 611
Hpy166II	GTNNAC	8 cut(s)	1571, 1588, 2215, 2333, 2593, 2909, 2964, 3010
Hpy188III	TCNNGA	9 cut(s)	391, 1214, 1541, 1808, 1994, 2045, 2549, 2759, 2777
Hpy8I	GTNNAC	8 cut(s)	1571, 1588, 2215, 2333, 2593, 2909, 2964, 3010
Hpy99I	CGWCG	3 cut(s)	53, 293, 467
HpyAV	CCTTC	6 cut(s)	319, 583, 720, 1371, 1960, 2691
HpyCH4III	ACNGT	4 cut(s)	131, 978, 1614, 2001
HpyCH4IV	ACGT	3 cut(s)	1749, 2063, 3070
HpyF10VI	GCNNNNNNNGC	7 cut(s)	70, 219, 682, 816, 1140, 1981, 2604
HpyF3I	CTNAG	5 cut(s)	377, 1425, 2528, 2693, 2898
HpySE526I	ACGT	3 cut(s)	1749, 2063, 3070
Hsp92I	GRCGYC	1 cut(s)	176
HspAI	GCGC	1 cut(s)	17
Ksp22I	TGATCA	1 cut(s)	2761
KspAI	GTTAAC	2 cut(s)	2215, 2964
LguI	GCTCTTC	2 cut(s)	335, 1068
LmnI	GCTCC	4 cut(s)	753, 1230, 1429, 1815
Lsp1109I	GCAGC	9 cut(s)	6, 222, 1100, 1118, 1160, 1539, 2071, 2135, 2585
LweI	GCATC	8 cut(s)	913, 1379, 2082, 2415, 2476, 2542, 2864, 2964
MaeI	CTAG	7 cut(s)	1046, 1380, 1824, 2120, 2319, 2448, 3017
MaeII	ACGT	3 cut(s)	1749, 2063, 3070
MaeIII	GTNAC	6 cut(s)	125, 156, 1445, 2277, 2323, 2786
MbiI	CCGCTC	1 cut(s)	756
MflI	RGATCY	3 cut(s)	1159, 1216, 1780
MhlI	GDGCHC	5 cut(s)	244, 1682, 1704, 2035, 2644
MlyI	GAGTC	6 cut(s)	87, 164, 237, 2192, 2462, 2774
MmeI	TCCRAC	2 cut(s)	312, 1466
Mph1103I	ATGCAT	1 cut(s)	2430
MroXI	GAANNNNTTC	2 cut(s)	800, 2001
MslI	CAYNNNNRTG	3 cut(s)	368, 2706, 2739
MspA1I	CMGCKG	2 cut(s)	213, 2598
MspI	CCGG	5 cut(s)	23, 43, 55, 555, 611
MspR9I	CCNGG	2 cut(s)	1499, 2467
Mva1269I	GAATGC	1 cut(s)	905
MvaI	CCWGG	2 cut(s)	1499, 2467
MvnI	CGCG	1 cut(s)	622
MwoI	GCNNNNNNNGC	7 cut(s)	70, 219, 682, 816, 1140, 1981, 2604
NdeI	CATATG	1 cut(s)	2203
NlaIV	GGNNCC	7 cut(s)	401, 568, 731, 1232, 1782, 2125, 2641
NmeAIII	GCCGAG	2 cut(s)	567, 2974
NmuCI	GTSAC	4 cut(s)	125, 156, 1445, 2786
NsiI	ATGCAT	1 cut(s)	2430
NspI	RCATGY	5 cut(s)	867, 1558, 2481, 2744, 2915
PaeI	GCATGC	2 cut(s)	867, 2481
PagI	TCATGA	1 cut(s)	2548
PciI	ACATGT	2 cut(s)	1554, 2911
PciSI	GCTCTTC	2 cut(s)	335, 1068
PcsI	WCGNNNNNNNCGW	2 cut(s)	539, 2151
PctI	GAATGC	1 cut(s)	905
PdmI	GAANNNNTTC	2 cut(s)	800, 2001
PfeI	GAWTC	7 cut(s)	106, 1606, 1893, 2056, 2363, 2501, 2513
PflMI	CCANNNNNTGG	1 cut(s)	1867
PleI	GAGTC	6 cut(s)	86, 163, 236, 2192, 2461, 2774
PpsI	GAGTC	6 cut(s)	86, 163, 236, 2192, 2461, 2774
Ppu21I	YACGTR	1 cut(s)	3071
PpuMI	RGGWCCY	1 cut(s)	2123
PscI	ACATGT	2 cut(s)	1554, 2911
PshAI	GACNNNNGTC	1 cut(s)	2784
Psp124BI	GAGCTC	1 cut(s)	2035
Psp5II	RGGWCCY	1 cut(s)	2123
Psp6I	CCWGG	2 cut(s)	1497, 2465
PspFI	CCCAGC	1 cut(s)	188
PspGI	CCWGG	2 cut(s)	1497, 2465
PspN4I	GGNNCC	7 cut(s)	401, 568, 731, 1232, 1782, 2125, 2641
PspPI	GGNCC	8 cut(s)	25, 45, 358, 552, 1588, 1930, 2123, 3034
PspPPI	RGGWCCY	1 cut(s)	2123
PstNI	CAGNNNCTG	3 cut(s)	682, 1547, 2489
PsuI	RGATCY	3 cut(s)	1159, 1216, 1780
PvuII	CAGCTG	1 cut(s)	2598
RsaI	GTAC	7 cut(s)	833, 1358, 1377, 1752, 1904, 2166, 2910
RsaNI	GTAC	7 cut(s)	832, 1357, 1376, 1751, 1903, 2165, 2909
RseI	CAYNNNNRTG	3 cut(s)	368, 2706, 2739
Rsr2I	CGGWCCG	2 cut(s)	45, 552
RsrII	CGGWCCG	2 cut(s)	45, 552
SacI	GAGCTC	1 cut(s)	2035
SalI	GTCGAC	1 cut(s)	2591
SapI	GCTCTTC	2 cut(s)	335, 1068
Sau96I	GGNCC	8 cut(s)	25, 45, 358, 552, 1588, 1930, 2123, 3034
ScaI	AGTACT	1 cut(s)	1904
SchI	GAGTC	6 cut(s)	87, 164, 237, 2192, 2462, 2774
ScrFI	CCNGG	2 cut(s)	1499, 2467
SduI	GDGCHC	5 cut(s)	244, 1682, 1704, 2035, 2644
SfaNI	GCATC	8 cut(s)	913, 1379, 2082, 2415, 2476, 2542, 2864, 2964
SfcI	CTRYAG	1 cut(s)	974
SinI	GGWCC	6 cut(s)	25, 45, 358, 552, 1588, 2123
SmiMI	CAYNNNNRTG	3 cut(s)	368, 2706, 2739
SmlI	CTYRAG	4 cut(s)	161, 1808, 2782, 2801
SmoI	CTYRAG	4 cut(s)	161, 1808, 2782, 2801
SphI	GCATGC	2 cut(s)	867, 2481
SspMI	CTAG	7 cut(s)	1046, 1380, 1824, 2120, 2319, 2448, 3017
SstI	GAGCTC	1 cut(s)	2035
StyD4I	CCNGG	2 cut(s)	1497, 2465
StyI	CCWWGG	3 cut(s)	118, 2622, 2825
TaaI	ACNGT	4 cut(s)	131, 978, 1614, 2001
TaiI	ACGT	3 cut(s)	1752, 2066, 3073
TaqI	TCGA	8 cut(s)	390, 651, 742, 785, 877, 1213, 1609, 2592
TaqII	GACCGA	3 cut(s)	62, 540, 627
TatI	WGTACW	3 cut(s)	1902, 2164, 2908
TauI	GCSGC	2 cut(s)	42, 477
TfiI	GAWTC	7 cut(s)	106, 1606, 1893, 2056, 2363, 2501, 2513
TscAI	CASTG	7 cut(s)	1133, 1635, 1721, 1791, 1868, 1962, 2184
TseFI	GTSAC	4 cut(s)	125, 156, 1445, 2786
TseI	GCWGC	9 cut(s)	19, 210, 1113, 1131, 1173, 1527, 2084, 2148, 2598
Tsp45I	GTSAC	4 cut(s)	125, 156, 1445, 2786
TspGWI	ACGGA	3 cut(s)	259, 382, 2966
TspRI	CASTG	7 cut(s)	1133, 1635, 1721, 1791, 1868, 1962, 2184
Van91I	CCANNNNNTGG	1 cut(s)	1867
VpaK11BI	GGWCC	6 cut(s)	25, 45, 358, 552, 1588, 2123
XagI	CCTNNNNNAGG	1 cut(s)	2118
XapI	RAATTY	8 cut(s)	256, 352, 1535, 1843, 2711, 2723, 2810, 2893
XceI	RCATGY	5 cut(s)	867, 1558, 2481, 2744, 2915
XcmI	CCANNNNNNNNNTGG	2 cut(s)	508, 1598
XmiI	GTMKAC	1 cut(s)	2592
XmnI	GAANNNNTTC	2 cut(s)	800, 2001
XspI	CTAG	7 cut(s)	1046, 1380, 1824, 2120, 2319, 2448, 3017
ZrmI	AGTACT	1 cut(s)	1904
Zsp2I	ATGCAT	1 cut(s)	2430

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.