Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0003373

Physical Location & Seq

Genomic Coordinates Forward (+)

4164186 .. 4170079

Gene Structure 5894 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031332

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3144 bp

ATGGACCCGCTGGCCGGACCGGTGCCGGAGCATATGCGGTGCACGAGGACGGACGGCCGGCAATGGCGGTGCAAGAGGAGAGTGATGGAGAACATGAAGCTCTGCGAGATTCACTACCTGCAGGGACGGCACCGGCAGTACAGGGAGAAGGTGCCGGAGGAGCTGAAGATTCAGAGGAAGACGCAGACGGCGCCGTGCGAGGATCGCGACGGCGGCGTCGGCGGTGGGGAAGGCGGAGGAATTAGGGCAAAGAAAGTGGAGGATTTGGTGACGCTGCTGAAGAGGAAGAGGGCGGCGGAGGAGGCGGCGCCGGTGAAGATAAAGAAGCCGAAGGTGAAGAAGAAGAAGAAGAAGAAGAAGAGGAGAGGTGGGGAGAAGAAGTGTGAGTTGAATTTGGAGCTGATAAGGATGGTGCTGAAGAGGGAGGTGGAGAAAAGGAACACGCCGCAGAAGAAGAAGAAGAAGATGAGGATTTTGGTGGAGGAGGAGGGGAGTGAGGGTGAGGAGGGCCTTGATCTGACCAGAGACTTGCCTAATGGAAGAATGGCCATTTCGTCTTCTTCGTCGCAGTCTTTGGGGGGCAATGTAGGTTCCAATTCGGGCTCTGATGTTAGGGTTGGGGCCGAATTAGGGCCCCCGGTGTCTACTACTACGCGGCGGTGCTTTCGGTCCAAGAACATCGAGCCAATGCCTGTTGGCACAGTGCAGGTTTTCCCATATGGTGATGAGCATGAGGGGAGATTGAGGAAGGGTAAGAGGAAGAGGTGCCATTGGTGTCAGAGAAGTGGAAGTAGTGCTTCTTCCAGCTTGATTAAGTGTTCGAGTTGTAAGAAGCACTTGTTTTGCTTGAGCTGCATCAAAGAAAGGTATTTTGATACTCAAGAAGAAGTCAAAATGGCATGTCCAGTTTGTCGAGGAACTTGCCCCTGTAAGGAATGCTCTGAAAATCAACCGAAAGATGCTGAAAGTAAGGATTATGTGGGGGTTAAAAATAAAGTTGAAATTATATTACACTTTCACTACCTTGTCTGTATGCTTCTCCCTGTGCTGAAACAAATCAACCAAGATCAGAAATTTGAGCTGGAAGCAGAGGCAAAAATGAGAGGGAAAAAGCTCTCCGAAGTTTATATCAAGCAGGCTGAATTTAGCTGCAGTGAGCAAAACAGCTGCAATAAATGCAAAGCTCCCATTGTGGATCTTCACAGAAGCTGCCAAAATTGTTCCTATAACCTTTGTTTGAGCTGTTGTCGAGATCATTTTCAGGGGACTTTACGGGGAGGTATAAATACATTTCTCGCAAAGTGCTCTAATATAAATGATACTTGGGTCTCTGGTAAGGGGAAAGTTCAGAAAAGGCCAATAACTACCCATAAGCAAAATGTCCGGTACCTGTCGTCATTTGCATCAACTAACTGGAATGCTTGTAATGCTGTTGACGGTATATCTTGCCCGCCCAAGGAGTTTGGTGGTTGTGGTGACAGCTTCCTTGATTTGAGATGTTTTTTCCCTTTAAGTTGGATCAAAGAGCTAGAAGTAAGTGCTGAAGAAATAGTTTGCAGCTATGAGTTTCCAGAAACTTCAGACATGTCATTGAGCTGCCCTCTATGTTTGGACACGGACCAGAATGTCGATGGACTTCAACAGTTGCAAGAAGCTGCTTTGAGAGAAAATTCAAATGATAACTATCTATTCTACCCCACGCTTTCAGACGTACATGGTGATAATGTTGAACATTTTCAGAAGCACTGGAGTAAAGGTCATCCTGTTATAGTCCGAGATGTCCTTCGGAGTACGTCAGATTTGAGCTGGGATCCAGTATTCATGTTCTGCACTTATCTTGAGAGGAGCATTGCTAGATATGAAAATAACAAGAATCCACACGAAGCTATCCACTGTTTGGATTGGTGTGAGGTGGAAATGGGGATCAGGCAGTATTTTATGGGTTCATTGAACGGGCGAACACAAAAAGACATGTGGAGTGAGACCCTGAAATTGAAGGGTTGGCTTTCTTCACAATTATTTCAAGAACAATTTCCAGCTCATTATGCTGAAATCATCCAAGCTCTACCACTTCAAGAGTACATGAATCCCTCATCTGGTCTTTTAAATGTTGCTGCAAGGATGCCACAGGAAATCCCAAAACCTGATCTAGGTCCTTGTGTTTATATTTCGTATGGCTGCAGTGAGCAGTTTGTACAGGCTGATTCAGTGATAAAGTTGTGTTATGACTCATATGATGTGGTTAACATTTTGGCACATACATCAGATGCCCCTATTTCTGATGAACAAGTTTCTAAGGTAAGAAAGTTACTGAAAAGGCACAGTCAAAATCAGAGGGAGTCTTCTAGGAATACTTCTGAACAAACTAAAGCAAAAAAAGTGAATGGAAATTCAGCATTGCATAGTCAAAACAAGGAACAAGTGAGGTCACATAATGTGATTGGAGAAGAGATGCATTTACGGAAAAGAATTGCTAGAGAATCTTGCTTCTCTGCTGCAACGCGTGAAGAATGCACTAGGAATCTCAAAGAGAGTAATATGTCGCTTGATGGGGAATCTGATGTTGATTCTGAGGCCACGTTGAGTTGCTCTGGGTCTATCAATGGTGATGAAACATCTGAAGATGAGGAGTCACCGGATCTCCTTGAAAGCTCAAATGGCCATGAAAAACCCTTTGTAGAGTCATGCACTGCACAGTGGGATGTTTTCCGCCGGCAAGATGTTCCGAAACTTGTAGAATATCTCAGAAGGCACTGTAATGAATTTACTCGTAAATATGACGTTCATAAACATGTTGCTCATCCAATTCTTGATCAGAGTTTCTTTCTAGACTCGATTCACAAAATAAGACTCAAGGAAGAATTTAAAATTGAACCTTGGACTTTTGAGCAACACATTGGAGAAGCTGTCATCATCCCTGCTGGATGCCCGTACCAGATTAGTAGTCCTAAGTCTTGTGTACATGTGGTGCTTGATTTTATGTCTCCCGAAAATGTTACCGAGTGCATCCAGTTGACAGATGAAGTTCGCCTGCTTCCAGAAGATCATAAAGCAAAAGTGGACAAGCTGGAGGTGAAGCGAATGGCACTCTATAGTATTAGTTCAGCAATCAAAGAAATACGTGAGCTTACTTGTGCAATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000785 GO:0000976 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006325 GO:0006355 GO:0006464 GO:0006482 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008214 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016043 GO:0016569 GO:0016570 GO:0016577 GO:0019219 GO:0019222 GO:0019538 GO:0031323 GO:0031326 GO:0031490 GO:0031974 GO:0031981 GO:0032451 GO:0032452 GO:0032454 GO:0033169 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0070076 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0097159 GO:0140096 GO:1901363 GO:1901564 GO:1903506 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04714 map04714

Pfam Domains

Protein Families

JmjC WRC

Protein Analysis

1048

Amino Acids

119.13

Weight (kDa)

8.29

Isoelectric Point (pI)

59.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRC	PF08879	9 - 50	2.8e-16	WRC
JmjC	PF02373	895 - 993	3.8e-11	JmjC domain, hydroxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003373)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21430
fragaria_vesca	FvH4_3g03760 FvH4_3g03760 FvH4_3g03760 FvH4_3g03760 FvH4_3g03760 FvH4_3g03760
malus_domestica	MD05G1326700.v1.1 MD10G1304800.v1.1
prunus_persica	Prupe.4G034800_v2.0.a1 Prupe.4G034800_v2.0.a1 Prupe.4G034800_v2.0.a1 Prupe.4G034800_v2.0.a1 Prupe.4G034800_v2.0.a1 Prupe.4G034800_v2.0.a1 Prupe.4G034800_v2.0.a1
pyrus_communis	pycom05g30110 pycom10g25720
rosa_chinensis	RchiOBHm_Chr5g0005871
rosa_laevigata	RLG00000016376 RLG00000031332 RLG00000031334 RLG00000031337 RLG00000031345 RLG00000031346 RLG00000031385
rosa_multiflora	Rmu_sc0002694.1_g000004
rosa_roxburghii	Rroxscaffold_1G00070030
rosa_rugosa	Rorug04G0418700 Rorug04G0418800 Rorug04G0418900
rosa_samantha	Rh5AG046200 Rh5BG044900 Rh5CG053800 Rh5DG044700
rosa_wichuraiana	Rw5G004640

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	215, 1625
Acc36I	ACCTGC	2 cut(s)	126, 697
Acc65I	GGTACC	1 cut(s)	1386
AccB1I	GGYRCC	7 cut(s)	22, 129, 151, 190, 307, 765, 1386
AccB7I	CCANNNNNTGG	1 cut(s)	1897
AccI	GTMKAC	1 cut(s)	644
AccII	CGCG	3 cut(s)	207, 655, 2503
AclWI	GGATC	7 cut(s)	210, 1203, 1526, 1805, 1818, 1931, 2646
AcoI	YGGCCR	4 cut(s)	12, 55, 546, 2659
AcsI	RAATTY	7 cut(s)	391, 1073, 1142, 1669, 2389, 2762, 2861
AcuI	CTGAAG	6 cut(s)	185, 299, 437, 1563, 1563, 2640
AcyI	GRCGYC	3 cut(s)	191, 216, 308
AfaI	GTAC	8 cut(s)	140, 1388, 1713, 1792, 2081, 2196, 2933, 2961
AfiI	CCNNNNNNNGG	7 cut(s)	14, 630, 931, 1456, 1897, 2096, 2150
AflIII	ACRYGT	5 cut(s)	1584, 1971, 2501, 2791, 2962
AgeI	ACCGGT	1 cut(s)	19
Alw21I	GWGCWC	2 cut(s)	44, 1307
Alw26I	GTCTC	4 cut(s)	519, 1333, 1976, 2988
Alw44I	GTGCAC	1 cut(s)	40
AlwI	GGATC	7 cut(s)	210, 1203, 1526, 1805, 1818, 1931, 2646
AlwNI	CAGNNNCTG	1 cut(s)	1209
AoxI	GGCC	9 cut(s)	12, 55, 508, 546, 621, 632, 1355, 2574, 2659
ApaI	GGGCCC	1 cut(s)	636
ApaLI	GTGCAC	1 cut(s)	40
ApoI	RAATTY	7 cut(s)	391, 1073, 1142, 1669, 2389, 2762, 2861
AsiGI	ACCGGT	1 cut(s)	19
Asp700I	GAANNNNTTC	2 cut(s)	1734, 2031
Asp718I	GGTACC	1 cut(s)	1386
AspLEI	GCGC	2 cut(s)	193, 310
AspS9I	GGNCC	9 cut(s)	4, 17, 508, 621, 632, 633, 669, 1618, 2153
AsuC2I	CCSGG	1 cut(s)	638
AvaII	GGWCC	5 cut(s)	4, 17, 669, 1618, 2153
BaeGI	GKGCMC	2 cut(s)	44, 636
BaeI	ACNNNNGTAYC	2 cut(s)	1311, 1344
BalI	TGGCCA	2 cut(s)	548, 2661
BamHI	GGATCC	1 cut(s)	1810
BanI	GGYRCC	7 cut(s)	22, 129, 151, 190, 307, 765, 1386
BanII	GRGCYC	2 cut(s)	605, 636
BarI	GAAGNNNNNNTAC	2 cut(s)	784, 816
BauI	CACGAG	1 cut(s)	43
BbsI	GAAGAC	3 cut(s)	185, 549, 2334
Bbv12I	GWGCWC	2 cut(s)	44, 1307
BccI	CCATC	4 cut(s)	79, 403, 1625, 2543
BceAI	ACGGC	5 cut(s)	70, 143, 178, 204, 226
BcgI	CGANNNNNNTGC	2 cut(s)	903, 937
BclI	TGATCA	1 cut(s)	2812
BcnI	CCSGG	1 cut(s)	638
BcoDI	GTCTC	4 cut(s)	519, 1333, 1976, 2988
BfaI	CTAG	7 cut(s)	1529, 1854, 2150, 2346, 2475, 2517, 2828
BfmI	CTRYAG	4 cut(s)	119, 1150, 2179, 3091
BfoI	RGCGCY	2 cut(s)	194, 311
BfuAI	ACCTGC	2 cut(s)	126, 697
Bme1390I	CCNGG	1 cut(s)	638
Bme18I	GGWCC	5 cut(s)	4, 17, 669, 1618, 2153
BmgT120I	GGNCC	9 cut(s)	4, 17, 508, 621, 632, 633, 669, 1618, 2153
BmrFI	CCNGG	1 cut(s)	638
BmsI	GCATC	8 cut(s)	864, 949, 1412, 2112, 2257, 2442, 2915, 3015
BpiI	GAAGAC	3 cut(s)	185, 549, 2334
BplI	GAGNNNNNCTC	2 cut(s)	1585, 1617
BpmI	CTGGAG	2 cut(s)	1768, 3089
BpuEI	CTTGAG	4 cut(s)	864, 868, 1859, 2837
BpuMI	CCSGG	1 cut(s)	638
BsaAI	YACGTR	1 cut(s)	3122
BsaBI	GATNNNNATC	1 cut(s)	1683
BsaHI	GRCGYC	3 cut(s)	191, 216, 308
BsaI	GGTCTC	2 cut(s)	1333, 1976
BsaJI	CCNNGG	3 cut(s)	636, 1455, 2876
BsaWI	WCCGGW	3 cut(s)	19, 1383, 2635
Bsc4I	CCNNNNNNNGG	7 cut(s)	14, 630, 931, 1456, 1897, 2096, 2150
Bse118I	RCCGGY	5 cut(s)	19, 57, 132, 310, 2712
Bse1I	ACTGG	5 cut(s)	905, 1418, 1751, 1814, 3010
Bse3DI	GCAATG	5 cut(s)	68, 589, 1848, 2396, 3144
Bse8I	GATNNNNATC	1 cut(s)	1683
BseDI	CCNNGG	3 cut(s)	636, 1455, 2876
BseGI	GGATG	9 cut(s)	414, 1759, 2055, 2127, 2707, 2800, 2913, 2930, 3006
BseJI	GATNNNNATC	1 cut(s)	1683
BseLI	CCNNNNNNNGG	7 cut(s)	14, 630, 931, 1456, 1897, 2096, 2150
BseMI	GCAATG	5 cut(s)	68, 589, 1848, 2396, 3144
BseMII	CTCAG	2 cut(s)	2562, 2758
BseNI	ACTGG	5 cut(s)	905, 1418, 1751, 1814, 3010
BseRI	GAGGAG	9 cut(s)	91, 173, 314, 376, 497, 500, 518, 1858, 2642
BseSI	GKGCMC	2 cut(s)	44, 636
BseX3I	CGGCCG	1 cut(s)	55
BseYI	CCCAGC	1 cut(s)	1806
BsgI	GTGCAG	3 cut(s)	725, 1813, 2676
Bsh1236I	CGCG	3 cut(s)	207, 655, 2503
Bsh1285I	CGRYCG	1 cut(s)	58
BshFI	GGCC	9 cut(s)	14, 57, 510, 548, 623, 634, 1357, 2576, 2661
BshNI	GGYRCC	7 cut(s)	22, 129, 151, 190, 307, 765, 1386
BshTI	ACCGGT	1 cut(s)	19
BsiEI	CGRYCG	1 cut(s)	58
BsiHKAI	GWGCWC	2 cut(s)	44, 1307
BslFI	GGGAC	2 cut(s)	138, 1279
BslI	CCNNNNNNNGG	7 cut(s)	14, 630, 931, 1456, 1897, 2096, 2150
BsmAI	GTCTC	4 cut(s)	519, 1333, 1976, 2988
BsmFI	GGGAC	2 cut(s)	138, 1279
BsmI	GAATGC	3 cut(s)	941, 1423, 2516
BsnI	GGCC	9 cut(s)	14, 57, 510, 548, 623, 634, 1357, 2576, 2661
Bso31I	GGTCTC	2 cut(s)	1333, 1976
Bsp120I	GGGCCC	1 cut(s)	632
Bsp1286I	GDGCHC	4 cut(s)	44, 605, 636, 1307
Bsp1407I	TGTACA	2 cut(s)	2194, 2959
Bsp68I	TCGCGA	1 cut(s)	207
BspANI	GGCC	9 cut(s)	14, 57, 510, 548, 623, 634, 1357, 2576, 2661
BspCNI	CTCAG	2 cut(s)	2563, 2757
BspFNI	CGCG	3 cut(s)	207, 655, 2503
BspMAI	CTGCAG	3 cut(s)	123, 1154, 2183
BspMI	ACCTGC	2 cut(s)	126, 697
BspPI	GGATC	7 cut(s)	210, 1203, 1526, 1805, 1818, 1931, 2646
BspT107I	GGYRCC	7 cut(s)	22, 129, 151, 190, 307, 765, 1386
BspTNI	GGTCTC	2 cut(s)	1333, 1976
BsrDI	GCAATG	5 cut(s)	68, 589, 1848, 2396, 3144
BsrFI	RCCGGY	5 cut(s)	19, 57, 132, 310, 2712
BsrGI	TGTACA	2 cut(s)	2194, 2959
BsrI	ACTGG	5 cut(s)	905, 1418, 1751, 1814, 3010
BssAI	RCCGGY	5 cut(s)	19, 57, 132, 310, 2712
BssECI	CCNNGG	3 cut(s)	636, 1455, 2876
BssNI	GRCGYC	3 cut(s)	191, 216, 308
BssSI	CACGAG	1 cut(s)	43
BssT1I	CCWWGG	2 cut(s)	1455, 2876
Bst2BI	CACGAG	1 cut(s)	43
Bst4CI	ACNGT	7 cut(s)	703, 1439, 1644, 1895, 2324, 2697, 2756
Bst6I	CTCTTC	6 cut(s)	275, 281, 353, 413, 755, 2442
BstACI	GRCGYC	3 cut(s)	191, 216, 308
BstAPI	GCANNNNNTGC	1 cut(s)	1176
BstAUI	TGTACA	2 cut(s)	2194, 2959
BstBAI	YACGTR	1 cut(s)	3122
BstC8I	GCNNGC	6 cut(s)	12, 59, 1137, 1451, 2714, 3032
BstDEI	CTNAG	4 cut(s)	2295, 2571, 2744, 2949
BstENI	CCTNNNNNAGG	1 cut(s)	2148
BstF5I	GGATG	9 cut(s)	414, 1759, 2055, 2127, 2707, 2800, 2913, 2930, 3006
BstFNI	CGCG	3 cut(s)	207, 655, 2503
BstH2I	RGCGCY	2 cut(s)	194, 311
BstHHI	GCGC	2 cut(s)	193, 310
BstMAI	GTCTC	4 cut(s)	519, 1333, 1976, 2988
BstMCI	CGRYCG	1 cut(s)	58
BstNSI	RCATGY	5 cut(s)	903, 1588, 1975, 2795, 2966
BstSCI	CCNGG	1 cut(s)	636
BstSFI	CTRYAG	4 cut(s)	119, 1150, 2179, 3091
BstSLI	GKGCMC	2 cut(s)	44, 636
BstUI	CGCG	3 cut(s)	207, 655, 2503
BstV2I	GAAGAC	3 cut(s)	185, 549, 2334
BstX2I	RGATCY	3 cut(s)	1195, 1810, 2638
BstYI	RGATCY	3 cut(s)	1195, 1810, 2638
BstZI	CGGCCG	1 cut(s)	55
BsuRI	GGCC	9 cut(s)	14, 57, 510, 548, 623, 634, 1357, 2576, 2661
BtsCI	GGATG	9 cut(s)	414, 1759, 2055, 2127, 2707, 2800, 2913, 2930, 3006
BtsI	GCAGTG	3 cut(s)	1159, 2188, 2688
BtsIMutI	CAGTG	9 cut(s)	708, 1159, 1744, 1891, 2188, 2214, 2688, 2702, 2752
BtuMI	TCGCGA	1 cut(s)	207
BveI	ACCTGC	2 cut(s)	126, 697
Cac8I	GCNNGC	6 cut(s)	12, 59, 1137, 1451, 2714, 3032
CaiI	CAGNNNCTG	1 cut(s)	1209
CfoI	GCGC	2 cut(s)	193, 310
Cfr10I	RCCGGY	5 cut(s)	19, 57, 132, 310, 2712
Cfr13I	GGNCC	9 cut(s)	4, 17, 508, 621, 632, 633, 669, 1618, 2153
CpoI	CGGWCCG	1 cut(s)	17
CseI	GACGC	3 cut(s)	190, 205, 280
Csp6I	GTAC	8 cut(s)	139, 1387, 1712, 1791, 2080, 2195, 2932, 2960
CspAI	ACCGGT	1 cut(s)	19
CspCI	CAANNNNNGTGG	2 cut(s)	237, 272
CspI	CGGWCCG	1 cut(s)	17
CviQI	GTAC	8 cut(s)	139, 1387, 1712, 1791, 2080, 2195, 2932, 2960
DdeI	CTNAG	4 cut(s)	2295, 2571, 2744, 2949
DinI	GGCGCC	2 cut(s)	192, 309
DraI	TTTAAA	2 cut(s)	2106, 2866
DrdI	GACNNNNNNGTC	2 cut(s)	215, 1625
DseDI	GACNNNNNNGTC	2 cut(s)	215, 1625
EaeI	YGGCCR	4 cut(s)	12, 55, 546, 2659
EagI	CGGCCG	1 cut(s)	55
Eam1104I	CTCTTC	6 cut(s)	275, 281, 353, 413, 755, 2442
EarI	CTCTTC	6 cut(s)	275, 281, 353, 413, 755, 2442
EciI	GGCGGA	3 cut(s)	249, 311, 2699
EclXI	CGGCCG	1 cut(s)	55
Eco130I	CCWWGG	2 cut(s)	1455, 2876
Eco24I	GRGCYC	2 cut(s)	605, 636
Eco31I	GGTCTC	2 cut(s)	1333, 1976
Eco47I	GGWCC	5 cut(s)	4, 17, 669, 1618, 2153
Eco52I	CGGCCG	1 cut(s)	55
Eco57I	CTGAAG	6 cut(s)	185, 299, 437, 1563, 1563, 2640
EcoNI	CCTNNNNNAGG	1 cut(s)	2148
EcoO109I	RGGNCCY	4 cut(s)	508, 632, 633, 2153
EcoT14I	CCWWGG	2 cut(s)	1455, 2876
EcoT22I	ATGCAT	1 cut(s)	2457
EcoT38I	GRGCYC	2 cut(s)	605, 636
EgeI	GGCGCC	2 cut(s)	192, 309
EheI	GGCGCC	2 cut(s)	192, 309
ErhI	CCWWGG	2 cut(s)	1455, 2876
FalI	AAGNNNNNCTT	4 cut(s)	781, 813, 821, 853
FaqI	GGGAC	2 cut(s)	138, 1279
FauI	CCCGC	2 cut(s)	15, 1458
FauNDI	CATATG	3 cut(s)	33, 718, 2233
FbaI	TGATCA	1 cut(s)	2812
FblI	GTMKAC	1 cut(s)	644
FokI	GGATG	9 cut(s)	421, 1746, 2042, 2134, 2714, 2787, 2900, 2937, 2993
FriOI	GRGCYC	2 cut(s)	605, 636
FspBI	CTAG	7 cut(s)	1529, 1854, 2150, 2346, 2475, 2517, 2828
GlaI	GCGC	2 cut(s)	192, 309
GsaI	CCCAGC	1 cut(s)	1810
GsuI	CTGGAG	2 cut(s)	1768, 3089
HaeII	RGCGCY	2 cut(s)	194, 311
HaeIII	GGCC	9 cut(s)	14, 57, 510, 548, 623, 634, 1357, 2576, 2661
HgaI	GACGC	3 cut(s)	190, 205, 280
HhaI	GCGC	2 cut(s)	193, 310
Hin1I	GRCGYC	3 cut(s)	191, 216, 308
Hin6I	GCGC	2 cut(s)	191, 308
HinP1I	GCGC	2 cut(s)	191, 308
HincII	GTYRAC	3 cut(s)	1435, 2245, 3015
HindII	GTYRAC	3 cut(s)	1435, 2245, 3015
HpaI	GTTAAC	1 cut(s)	2245
Hpy166II	GTNNAC	7 cut(s)	42, 645, 1435, 2245, 2960, 3015, 3061
Hpy8I	GTNNAC	7 cut(s)	42, 645, 1435, 2245, 2960, 3015, 3061
Hpy99I	CGWCG	3 cut(s)	212, 221, 568
HpyAV	CCTTC	7 cut(s)	142, 224, 325, 742, 1793, 1990, 2742
HpyCH4III	ACNGT	7 cut(s)	703, 1439, 1644, 1895, 2324, 2697, 2756
HpyCH4IV	ACGT	5 cut(s)	1710, 1793, 2579, 2781, 3121
HpyF3I	CTNAG	4 cut(s)	2295, 2571, 2744, 2949
HpySE526I	ACGT	5 cut(s)	1710, 1793, 2579, 2781, 3121
Hsp92I	GRCGYC	3 cut(s)	191, 216, 308
HspAI	GCGC	2 cut(s)	191, 308
KasI	GGCGCC	2 cut(s)	190, 307
KpnI	GGTACC	1 cut(s)	1390
KroI	GCCGGC	2 cut(s)	57, 2712
KroNI	GCCGGC	2 cut(s)	59, 2714
Ksp22I	TGATCA	1 cut(s)	2812
KspAI	GTTAAC	1 cut(s)	2245
LmnI	GCTCC	5 cut(s)	28, 160, 397, 1189, 1845
LweI	GCATC	8 cut(s)	864, 949, 1412, 2112, 2257, 2442, 2915, 3015
MaeI	CTAG	7 cut(s)	1529, 1854, 2150, 2346, 2475, 2517, 2828
MaeII	ACGT	5 cut(s)	1710, 1793, 2579, 2781, 3121
MaeIII	GTNAC	6 cut(s)	268, 1475, 2307, 2427, 2631, 2995
MflI	RGATCY	3 cut(s)	1195, 1810, 2638
MhlI	GDGCHC	4 cut(s)	44, 605, 636, 1307
MlsI	TGGCCA	2 cut(s)	548, 2661
MluI	ACGCGT	1 cut(s)	2501
MluNI	TGGCCA	2 cut(s)	548, 2661
Mly113I	GGCGCC	2 cut(s)	191, 308
MlyI	GAGTC	6 cut(s)	2222, 2348, 2639, 2690, 2825, 2844
MmeI	TCCRAC	1 cut(s)	1496
Mox20I	TGGCCA	2 cut(s)	548, 2661
Mph1103I	ATGCAT	1 cut(s)	2457
MroNI	GCCGGC	2 cut(s)	57, 2712
MroXI	GAANNNNTTC	2 cut(s)	1734, 2031
MscI	TGGCCA	2 cut(s)	548, 2661
MseI	TTAA	6 cut(s)	813, 987, 1511, 2105, 2244, 2865
MslI	CAYNNNNRTG	2 cut(s)	2757, 2790
Msp20I	TGGCCA	2 cut(s)	548, 2661
MspA1I	CMGCKG	2 cut(s)	10, 1167
MspR9I	CCNGG	1 cut(s)	638
Mva1269I	GAATGC	3 cut(s)	941, 1423, 2516
MvnI	CGCG	3 cut(s)	207, 655, 2503
NaeI	GCCGGC	2 cut(s)	59, 2714
NarI	GGCGCC	2 cut(s)	191, 308
NciI	CCSGG	1 cut(s)	638
NdeI	CATATG	3 cut(s)	33, 718, 2233
NgoMIV	GCCGGC	2 cut(s)	57, 2712
NmuCI	GTSAC	4 cut(s)	268, 1475, 2427, 2631
NruI	TCGCGA	1 cut(s)	207
NsiI	ATGCAT	1 cut(s)	2457
NspI	RCATGY	5 cut(s)	903, 1588, 1975, 2795, 2966
PciI	ACATGT	4 cut(s)	1584, 1971, 2791, 2962
PcsI	WCGNNNNNNNCGW	1 cut(s)	560
PctI	GAATGC	3 cut(s)	941, 1423, 2516
PdiI	GCCGGC	2 cut(s)	59, 2714
PdmI	GAANNNNTTC	2 cut(s)	1734, 2031
PflMI	CCANNNNNTGG	1 cut(s)	1897
PinAI	ACCGGT	1 cut(s)	19
PleI	GAGTC	6 cut(s)	2222, 2347, 2638, 2689, 2825, 2844
PluTI	GGCGCC	2 cut(s)	194, 311
PpsI	GAGTC	6 cut(s)	2222, 2347, 2638, 2689, 2825, 2844
Ppu21I	YACGTR	1 cut(s)	3122
PpuMI	RGGWCCY	1 cut(s)	2153
PscI	ACATGT	4 cut(s)	1584, 1971, 2791, 2962
Psp5II	RGGWCCY	1 cut(s)	2153
PspFI	CCCAGC	1 cut(s)	1806
PspOMI	GGGCCC	1 cut(s)	632
PspPI	GGNCC	9 cut(s)	4, 17, 508, 621, 632, 633, 669, 1618, 2153
PspPPI	RGGWCCY	1 cut(s)	2153
PstI	CTGCAG	3 cut(s)	123, 1154, 2183
PstNI	CAGNNNCTG	1 cut(s)	1209
PsuI	RGATCY	3 cut(s)	1195, 1810, 2638
PvuII	CAGCTG	1 cut(s)	1167
RruI	TCGCGA	1 cut(s)	207
RsaI	GTAC	8 cut(s)	140, 1388, 1713, 1792, 2081, 2196, 2933, 2961
RsaNI	GTAC	8 cut(s)	139, 1387, 1712, 1791, 2080, 2195, 2932, 2960
RseI	CAYNNNNRTG	2 cut(s)	2757, 2790
Rsr2I	CGGWCCG	1 cut(s)	17
RsrII	CGGWCCG	1 cut(s)	17
SaqAI	TTAA	6 cut(s)	813, 987, 1511, 2105, 2244, 2865
Sau96I	GGNCC	9 cut(s)	4, 17, 508, 621, 632, 633, 669, 1618, 2153
SbfI	CCTGCAGG	1 cut(s)	123
SchI	GAGTC	6 cut(s)	2222, 2348, 2639, 2690, 2825, 2844
ScrFI	CCNGG	1 cut(s)	638
SdaI	CCTGCAGG	1 cut(s)	123
SduI	GDGCHC	4 cut(s)	44, 605, 636, 1307
SfaNI	GCATC	8 cut(s)	864, 949, 1412, 2112, 2257, 2442, 2915, 3015
SfcI	CTRYAG	4 cut(s)	119, 1150, 2179, 3091
SfoI	GGCGCC	2 cut(s)	192, 309
SgrAI	CRCCGGYG	1 cut(s)	310
SinI	GGWCC	5 cut(s)	4, 17, 669, 1618, 2153
SmiMI	CAYNNNNRTG	2 cut(s)	2757, 2790
SmlI	CTYRAG	4 cut(s)	847, 879, 1838, 2852
SmoI	CTYRAG	4 cut(s)	847, 879, 1838, 2852
Sse8387I	CCTGCAGG	1 cut(s)	123
SspDI	GGCGCC	2 cut(s)	190, 307
SspMI	CTAG	7 cut(s)	1529, 1854, 2150, 2346, 2475, 2517, 2828
StyD4I	CCNGG	1 cut(s)	636
StyI	CCWWGG	2 cut(s)	1455, 2876
TaaI	ACNGT	7 cut(s)	703, 1439, 1644, 1895, 2324, 2697, 2756
TaiI	ACGT	5 cut(s)	1713, 1796, 2582, 2784, 3124
TaqI	TCGA	6 cut(s)	681, 821, 913, 1249, 1629, 2834
TaqII	GACCGA	1 cut(s)	657
TatI	WGTACW	4 cut(s)	138, 2079, 2194, 2959
TauI	GCSGC	5 cut(s)	216, 296, 308, 448, 658
Tru1I	TTAA	6 cut(s)	813, 987, 1511, 2105, 2244, 2865
Tru9I	TTAA	6 cut(s)	813, 987, 1511, 2105, 2244, 2865
TscAI	CASTG	9 cut(s)	708, 1159, 1751, 1898, 2188, 2214, 2695, 2702, 2759
TseFI	GTSAC	4 cut(s)	268, 1475, 2427, 2631
Tsp45I	GTSAC	4 cut(s)	268, 1475, 2427, 2631
TspGWI	ACGGA	3 cut(s)	65, 1631, 2476
TspRI	CASTG	9 cut(s)	708, 1159, 1751, 1898, 2188, 2214, 2695, 2702, 2759
Van91I	CCANNNNNTGG	1 cut(s)	1897
VneI	GTGCAC	1 cut(s)	40
VpaK11BI	GGWCC	5 cut(s)	4, 17, 669, 1618, 2153
XagI	CCTNNNNNAGG	1 cut(s)	2148
XapI	RAATTY	7 cut(s)	391, 1073, 1142, 1669, 2389, 2762, 2861
XbaI	TCTAGA	1 cut(s)	2827
XceI	RCATGY	5 cut(s)	903, 1588, 1975, 2795, 2966
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1628
XmiI	GTMKAC	1 cut(s)	644
XmnI	GAANNNNTTC	2 cut(s)	1734, 2031
XspI	CTAG	7 cut(s)	1529, 1854, 2150, 2346, 2475, 2517, 2828
Zsp2I	ATGCAT	1 cut(s)	2457

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.