Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003811

Physical Location & Seq

Genomic Coordinates Forward (+)

40917678 .. 40921341

Gene Structure 3664 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1331100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1266 bp

ATGGCTCTTCTGACGTCCTTCATCGAAGTCCACCCGTGCTCTCACTTCCCCATCCAGAACCTCCCGTACGGCGTCTTCAAGCCCCAACCGGGTTCGCCAGCTAGACCGGGCGTGGCCATCGGCGACTACGTCTTGGACCTCTCCGAAATCGCCGCCGCCGGGTTATTCAACGGTCCGATCCTCGTCAACTCCGATTGCTTTCATCAGCCTGATTTAAACAAGTTCTTGGCGCTTGGACGGCCTGCGTGGAAGGAAGCTCGCGCCACCATTCAGAAGCTGTTGTCATCTACTGAGCCGACATTGCGTGACAATGAAAGTTTGAGGCAGAAATCACTTCTTCCATTGAGCAAGGTAGAAATGGTTCTTCCGATTACAATAGGGGACTATACGGATTTCTTTTCGTCAATGCATCACGCGAAGAATTGCGGGACCATATTTCGCGGGCCACAGAACGCGATTCCACCAAACTGGTTCCACCTTCCAATTGCATATCATGGTCGAGCTTCTTCGATTGTTATCTCGGGAACGGACATTATTCGACCAAAAGGTCAAGGTGCTCCTACTGGAAACTCTCCACCGCCTTTTGGCCCTTCGTTGAAACTAGACTTTGAGCTCGAAATGGCTGCTATAGTTGGCCCTGGAAATGAGTTAGGAAAGCCGGTGGGTGTTAATGAAGCAGCAGATCACATCTTTGGACTTGTGTTGATGAATGACTGGAGTGCTAGAGATATTCAGGCATGGGAATATATTCCCCTGGGGCCTTTCCTTGGGAAAAGCTTTGGGACTACAATATCCCCTTGGATAGTGACGCTGGAAGCTCTAGAACCTTTTCTTTGTGATGCTCCCAAGCAGGACCCCCATCCGTTGCCCTATCTGGCTGAAAAAATCTCCCAAAATTATGACATTTCATTGGAGGTTCAAATCAAACCTGCTGGACAAGATGAGTCGCATGTGGTCACAAAGAGTAACTTCAATAATTTATACTGGACATTGACTCAACAGGTAGCCCACCACACTATCAACGGCTGCAACTTAAGGCCAGGCGATCTCCTTGGAACAGGGACTATCAGTGGGCCTGAGCCCGATTCTCTTGGATGCTTGTTAGAGTTGACGTGGAATGGACAAAAGCAGCTGTCATTAAATGGGACACCTCGTAAATTCTTAGAAGATGGTGATGAAGTCATCATTTCTGGGTTTAGCAAGGGAAATGGTTACAACGTTGGCTTTGGGACTTGCTCCGGAAAGATTGTTCCCTCACCTCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004334 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006520 GO:0006570 GO:0006572 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008219 GO:0009056 GO:0009063 GO:0009072 GO:0009074 GO:0009987 GO:0016054 GO:0016787 GO:0016822 GO:0016823 GO:0018958 GO:0019336 GO:0019439 GO:0019752 GO:0032787 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0071704 GO:0072329 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606 GO:1901615 GO:1901616 GO:1901999 GO:1902000

KEGG Pathways

Metabolic & Signaling

ko00350 ko00643 ko01100 ko01120 map00350 map00643 map01100 map01120

Pfam Domains

Protein Families

FAA_hydrolase FAA_hydrolase_N

Protein Analysis

422

Amino Acids

45.66

Weight (kDa)

5.63

Isoelectric Point (pI)

30.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAA_hydrolase_N	PF09298	19 - 123	4e-34	Fumarylacetoacetase N-terminal
FAA_hydrolase	PF01557	132 - 412	1.8e-38	Fumarylacetoacetate (FAA) hydrolase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003811)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12050
fragaria_vesca	FvH4_3g01030
malus_domestica	MD10G1331100.v1.1
prunus_persica	Prupe.4G011100_v2.0.a1
pyrus_communis	pycom10g28060
rosa_chinensis	RchiOBHm_Chr3g0477951 RchiOBHm_Chr3g0484131 RchiOBHm_Chr5g0001571 RchiOBHm_Chr5g0003621
rosa_laevigata	RLG00000030938 RLG00000030942
rosa_multiflora	Rmu_co8093718.1_g000001 Rmu_sc0003749.1_g000003 Rmu_sc0005991.1_g000008 Rmu_sc0006320.1_g000011 Rmu_sc0029518.1_g000002 Rmu_sc0040965.1_g000001
rosa_roxburghii	Rroxscaffold_1G00074840
rosa_rugosa	Rorug02G0302800 Rorug03G0164100 Rorug04G0391100
rosa_samantha	Rh3AG214900 Rh3AG258900 Rh3BG248500 Rh3CG242500 Rh3DG241900 Rh5AG012300 Rh5BG014900 Rh5BG015100 Rh5CG013300 Rh5DG012600 Rh5DG012700
rosa_wichuraiana	Rw3G019370 Rw5G001170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	546
AatII	GACGTC	1 cut(s)	17
Acc36I	ACCTGC	1 cut(s)	937
AccII	CGCG	4 cut(s)	261, 416, 441, 455
AccIII	TCCGGA	1 cut(s)	1238
AciI	CCGC	5 cut(s)	153, 156, 426, 441, 578
AclI	AACGTT	1 cut(s)	1218
AclWI	GGATC	1 cut(s)	172
AcoI	YGGCCR	1 cut(s)	114
AcsI	RAATTY	1 cut(s)	1157
AcyI	GRCGYC	2 cut(s)	14, 72
AfaI	GTAC	1 cut(s)	68
AfiI	CCNNNNNNNGG	4 cut(s)	89, 159, 584, 767
AflII	CTTAAG	1 cut(s)	1033
AgsI	TTSAA	5 cut(s)	79, 169, 598, 920, 973
AjiI	CACGTC	1 cut(s)	1113
AjnI	CCWGG	3 cut(s)	637, 753, 1039
AloI	GAACNNNNNNTCC	2 cut(s)	1233, 1265
AluBI	AGCT	8 cut(s)	101, 257, 277, 503, 613, 777, 818, 1132
AluI	AGCT	8 cut(s)	101, 257, 277, 503, 613, 777, 818, 1132
Alw21I	GWGCWC	3 cut(s)	41, 559, 615
AlwI	GGATC	1 cut(s)	172
AlwNI	CAGNNNCTG	1 cut(s)	277
Ama87I	CYCGRG	1 cut(s)	520
Aor13HI	TCCGGA	1 cut(s)	1238
AoxI	GGCC	8 cut(s)	114, 239, 443, 586, 634, 758, 1037, 1073
ApeKI	GCWGC	4 cut(s)	623, 677, 1026, 1129
ApoI	RAATTY	1 cut(s)	1157
Asp700I	GAANNNNTTC	3 cut(s)	360, 747, 828
AspLEI	GCGC	2 cut(s)	232, 263
AspS9I	GGNCC	9 cut(s)	136, 173, 429, 443, 587, 635, 758, 853, 1073
AsuC2I	CCSGG	3 cut(s)	90, 108, 160
AsuHPI	GGTGA	2 cut(s)	1184, 1248
AvaI	CYCGRG	1 cut(s)	520
AvaII	GGWCC	4 cut(s)	136, 173, 429, 853
BalI	TGGCCA	1 cut(s)	116
BanII	GRGCYC	2 cut(s)	615, 1083
BarI	GAAGNNNNNNTAC	2 cut(s)	59, 91
BbsI	GAAGAC	1 cut(s)	67
Bbv12I	GWGCWC	3 cut(s)	41, 559, 615
BbvI	GCAGC	4 cut(s)	610, 689, 1013, 1141
BccI	CCATC	4 cut(s)	59, 125, 867, 1163
BceAI	ACGGC	3 cut(s)	85, 254, 1039
BcgI	CGANNNNNNTGC	2 cut(s)	605, 639
BciT130I	CCWGG	3 cut(s)	639, 755, 1041
BcnI	CCSGG	3 cut(s)	90, 108, 160
BfaI	CTAG	4 cut(s)	102, 602, 723, 821
BfmI	CTRYAG	1 cut(s)	627
BfoI	RGCGCY	1 cut(s)	233
BfrI	CTTAAG	1 cut(s)	1033
BfuAI	ACCTGC	1 cut(s)	937
BisI	GCNGC	6 cut(s)	153, 156, 624, 678, 1027, 1130
BlsI	GCNGC	6 cut(s)	154, 157, 625, 679, 1028, 1131
Bme1390I	CCNGG	6 cut(s)	90, 108, 160, 639, 755, 1041
Bme18I	GGWCC	4 cut(s)	136, 173, 429, 853
BmeT110I	CYCGRG	1 cut(s)	520
BmgBI	CACGTC	1 cut(s)	1113
BmgT120I	GGNCC	9 cut(s)	136, 173, 429, 443, 587, 635, 758, 853, 1073
BmiI	GGNNCC	4 cut(s)	430, 473, 759, 855
BmrFI	CCNGG	6 cut(s)	90, 108, 160, 639, 755, 1041
BmsI	GCATC	3 cut(s)	418, 829, 1085
BpiI	GAAGAC	1 cut(s)	67
BpmI	CTGGAG	1 cut(s)	736
Bpu10I	CCTNAGC	1 cut(s)	1077
BpuMI	CCSGG	3 cut(s)	90, 108, 160
BsaBI	GATNNNNATC	1 cut(s)	515
BsaHI	GRCGYC	2 cut(s)	14, 72
BsaJI	CCNNGG	6 cut(s)	637, 753, 754, 766, 797, 1051
BsaWI	WCCGGW	1 cut(s)	1238
Bsc4I	CCNNNNNNNGG	4 cut(s)	89, 159, 584, 767
Bse118I	RCCGGY	1 cut(s)	658
Bse1I	ACTGG	4 cut(s)	473, 568, 719, 989
Bse3DI	GCAATG	1 cut(s)	299
Bse8I	GATNNNNATC	1 cut(s)	515
BseAI	TCCGGA	1 cut(s)	1238
BseBI	CCWGG	3 cut(s)	639, 755, 1041
BseDI	CCNNGG	6 cut(s)	637, 753, 754, 766, 797, 1051
BseGI	GGATG	3 cut(s)	51, 859, 1100
BseJI	GATNNNNATC	1 cut(s)	515
BseLI	CCNNNNNNNGG	4 cut(s)	89, 159, 584, 767
BseMI	GCAATG	1 cut(s)	299
BseMII	CTCAG	2 cut(s)	282, 1068
BseNI	ACTGG	4 cut(s)	473, 568, 719, 989
BseXI	GCAGC	4 cut(s)	610, 689, 1013, 1141
Bsh1236I	CGCG	4 cut(s)	261, 416, 441, 455
BshFI	GGCC	8 cut(s)	116, 241, 445, 588, 636, 760, 1039, 1075
BsiHKAI	GWGCWC	3 cut(s)	41, 559, 615
BsiHKCI	CYCGRG	1 cut(s)	520
BsiSI	CCGG	5 cut(s)	89, 107, 159, 659, 1239
BsiWI	CGTACG	1 cut(s)	66
BslFI	GGGAC	6 cut(s)	395, 442, 796, 1075, 1159, 1243
BslI	CCNNNNNNNGG	4 cut(s)	89, 159, 584, 767
BsmFI	GGGAC	6 cut(s)	395, 442, 796, 1075, 1159, 1243
BsnI	GGCC	8 cut(s)	116, 241, 445, 588, 636, 760, 1039, 1075
BsoBI	CYCGRG	1 cut(s)	520
Bsp1286I	GDGCHC	4 cut(s)	41, 559, 615, 1083
Bsp13I	TCCGGA	1 cut(s)	1238
Bsp143I	GATC	3 cut(s)	177, 682, 1045
BspACI	CCGC	5 cut(s)	153, 156, 426, 441, 578
BspANI	GGCC	8 cut(s)	116, 241, 445, 588, 636, 760, 1039, 1075
BspCNI	CTCAG	2 cut(s)	283, 1069
BspEI	TCCGGA	1 cut(s)	1238
BspFNI	CGCG	4 cut(s)	261, 416, 441, 455
BspLI	GGNNCC	4 cut(s)	430, 473, 759, 855
BspMI	ACCTGC	1 cut(s)	937
BspPI	GGATC	1 cut(s)	172
BspQI	GCTCTTC	1 cut(s)	12
BspTI	CTTAAG	1 cut(s)	1033
BsrDI	GCAATG	1 cut(s)	299
BsrFI	RCCGGY	1 cut(s)	658
BsrI	ACTGG	4 cut(s)	473, 568, 719, 989
BssAI	RCCGGY	1 cut(s)	658
BssECI	CCNNGG	6 cut(s)	637, 753, 754, 766, 797, 1051
BssMI	GATC	3 cut(s)	177, 682, 1045
BssNI	GRCGYC	2 cut(s)	14, 72
BssT1I	CCWWGG	3 cut(s)	766, 797, 1051
Bst2UI	CCWGG	3 cut(s)	639, 755, 1041
Bst4CI	ACNGT	1 cut(s)	173
Bst6I	CTCTTC	1 cut(s)	12
BstACI	GRCGYC	2 cut(s)	14, 72
BstAFI	CTTAAG	1 cut(s)	1033
BstC8I	GCNNGC	4 cut(s)	99, 243, 259, 443
BstDEI	CTNAG	3 cut(s)	291, 1077, 1162
BstF5I	GGATG	3 cut(s)	51, 859, 1100
BstFNI	CGCG	4 cut(s)	261, 416, 441, 455
BstH2I	RGCGCY	1 cut(s)	233
BstHHI	GCGC	2 cut(s)	232, 263
BstKTI	GATC	3 cut(s)	180, 685, 1048
BstMBI	GATC	3 cut(s)	177, 682, 1045
BstMWI	GCNNNNNNNGC	2 cut(s)	238, 301
BstNI	CCWGG	3 cut(s)	639, 755, 1041
BstNSI	RCATGY	1 cut(s)	953
BstSCI	CCNGG	6 cut(s)	88, 106, 158, 637, 753, 1039
BstSFI	CTRYAG	1 cut(s)	627
BstUI	CGCG	4 cut(s)	261, 416, 441, 455
BstV1I	GCAGC	4 cut(s)	610, 689, 1013, 1141
BstV2I	GAAGAC	1 cut(s)	67
BstXI	CCANNNNNNTGG	1 cut(s)	468
BsuRI	GGCC	8 cut(s)	116, 241, 445, 588, 636, 760, 1039, 1075
BtrI	CACGTC	1 cut(s)	1113
BtsCI	GGATG	3 cut(s)	51, 859, 1100
BtsIMutI	CAGTG	1 cut(s)	1075
BveI	ACCTGC	1 cut(s)	937
Cac8I	GCNNGC	4 cut(s)	99, 243, 259, 443
CaiI	CAGNNNCTG	1 cut(s)	277
CfoI	GCGC	2 cut(s)	232, 263
Cfr10I	RCCGGY	1 cut(s)	658
Cfr13I	GGNCC	9 cut(s)	136, 173, 429, 443, 587, 635, 758, 853, 1073
CpoI	CGGWCCG	1 cut(s)	173
CseI	GACGC	2 cut(s)	61, 817
Csp6I	GTAC	1 cut(s)	67
CspI	CGGWCCG	1 cut(s)	173
CviAII	CATG	3 cut(s)	494, 738, 950
CviQI	GTAC	1 cut(s)	67
DdeI	CTNAG	3 cut(s)	291, 1077, 1162
DpnI	GATC	3 cut(s)	179, 684, 1047
DpnII	GATC	3 cut(s)	177, 682, 1045
DraI	TTTAAA	1 cut(s)	216
DrdI	GACNNNNNNGTC	1 cut(s)	546
DseDI	GACNNNNNNGTC	1 cut(s)	546
EaeI	YGGCCR	1 cut(s)	114
Eam1104I	CTCTTC	1 cut(s)	12
EarI	CTCTTC	1 cut(s)	12
Ecl136II	GAGCTC	1 cut(s)	613
Eco130I	CCWWGG	3 cut(s)	766, 797, 1051
Eco24I	GRGCYC	2 cut(s)	615, 1083
Eco47I	GGWCC	4 cut(s)	136, 173, 429, 853
Eco53kI	GAGCTC	1 cut(s)	613
Eco88I	CYCGRG	1 cut(s)	520
EcoICRI	GAGCTC	1 cut(s)	613
EcoO109I	RGGNCCY	2 cut(s)	758, 853
EcoRII	CCWGG	3 cut(s)	637, 753, 1039
EcoT14I	CCWWGG	3 cut(s)	766, 797, 1051
EcoT22I	ATGCAT	1 cut(s)	411
EcoT38I	GRGCYC	2 cut(s)	615, 1083
ErhI	CCWWGG	3 cut(s)	766, 797, 1051
FaeI	CATG	3 cut(s)	497, 741, 953
FalI	AAGNNNNNCTT	2 cut(s)	953, 985
FaqI	GGGAC	6 cut(s)	395, 442, 796, 1075, 1159, 1243
FatI	CATG	3 cut(s)	493, 737, 949
FauI	CCCGC	2 cut(s)	419, 434
Fnu4HI	GCNGC	6 cut(s)	153, 156, 624, 678, 1027, 1130
FokI	GGATG	3 cut(s)	38, 846, 1107
FriOI	GRGCYC	2 cut(s)	615, 1083
Fsp4HI	GCNGC	6 cut(s)	153, 156, 624, 678, 1027, 1130
FspBI	CTAG	4 cut(s)	102, 602, 723, 821
GlaI	GCGC	2 cut(s)	231, 262
GluI	GCNGC	6 cut(s)	153, 156, 624, 678, 1027, 1130
GsuI	CTGGAG	1 cut(s)	736
HaeII	RGCGCY	1 cut(s)	233
HaeIII	GGCC	8 cut(s)	116, 241, 445, 588, 636, 760, 1039, 1075
HapII	CCGG	5 cut(s)	89, 107, 159, 659, 1239
HgaI	GACGC	2 cut(s)	61, 817
HhaI	GCGC	2 cut(s)	232, 263
Hin1I	GRCGYC	2 cut(s)	14, 72
Hin1II	CATG	3 cut(s)	497, 741, 953
Hin6I	GCGC	2 cut(s)	230, 261
HinP1I	GCGC	2 cut(s)	230, 261
HincII	GTYRAC	2 cut(s)	187, 1110
HindII	GTYRAC	2 cut(s)	187, 1110
HindIII	AAGCTT	1 cut(s)	775
HinfI	GANTC	4 cut(s)	457, 944, 994, 1085
HpaII	CCGG	5 cut(s)	89, 107, 159, 659, 1239
HphI	GGTGA	2 cut(s)	1184, 1248
Hpy166II	GTNNAC	3 cut(s)	31, 187, 1110
Hpy188I	TCNGA	6 cut(s)	12, 145, 177, 193, 273, 369
Hpy188III	TCNNGA	4 cut(s)	55, 522, 821, 1239
Hpy8I	GTNNAC	3 cut(s)	31, 187, 1110
HpyAV	CCTTC	4 cut(s)	28, 244, 488, 600
HpyCH4III	ACNGT	1 cut(s)	173
HpyCH4IV	ACGT	4 cut(s)	14, 129, 1112, 1218
HpyCH4V	TGCA	3 cut(s)	409, 488, 1029
HpyF10VI	GCNNNNNNNGC	2 cut(s)	238, 301
HpyF3I	CTNAG	3 cut(s)	291, 1077, 1162
HpySE526I	ACGT	4 cut(s)	14, 129, 1112, 1218
Hsp92I	GRCGYC	2 cut(s)	14, 72
Hsp92II	CATG	3 cut(s)	497, 741, 953
HspAI	GCGC	2 cut(s)	230, 261
Kpn2I	TCCGGA	1 cut(s)	1238
Kzo9I	GATC	3 cut(s)	177, 682, 1045
LguI	GCTCTTC	1 cut(s)	12
LmnI	GCTCC	3 cut(s)	562, 847, 1241
Lsp1109I	GCAGC	4 cut(s)	610, 689, 1013, 1141
LweI	GCATC	3 cut(s)	418, 829, 1085
MaeI	CTAG	4 cut(s)	102, 602, 723, 821
MaeII	ACGT	4 cut(s)	14, 129, 1112, 1218
MaeIII	GTNAC	5 cut(s)	305, 805, 955, 965, 1211
MalI	GATC	3 cut(s)	179, 684, 1047
MboI	GATC	3 cut(s)	177, 682, 1045
MboII	GAAGA	6 cut(s)	67, 329, 356, 430, 498, 1178
MfeI	CAATTG	1 cut(s)	483
MhlI	GDGCHC	4 cut(s)	41, 559, 615, 1083
MlsI	TGGCCA	1 cut(s)	116
MluCI	AATT	5 cut(s)	421, 483, 895, 976, 1157
MluNI	TGGCCA	1 cut(s)	116
MlyI	GAGTC	2 cut(s)	953, 988
MnlI	CCTC	7 cut(s)	71, 149, 191, 315, 907, 1161, 1264
Mox20I	TGGCCA	1 cut(s)	116
Mph1103I	ATGCAT	1 cut(s)	411
MroI	TCCGGA	1 cut(s)	1238
MroXI	GAANNNNTTC	3 cut(s)	360, 747, 828
MscI	TGGCCA	1 cut(s)	116
MseI	TTAA	4 cut(s)	215, 669, 1034, 1139
Msp20I	TGGCCA	1 cut(s)	116
MspA1I	CMGCKG	1 cut(s)	1132
MspCI	CTTAAG	1 cut(s)	1033
MspI	CCGG	5 cut(s)	89, 107, 159, 659, 1239
MspR9I	CCNGG	6 cut(s)	90, 108, 160, 639, 755, 1041
MunI	CAATTG	1 cut(s)	483
MvaI	CCWGG	3 cut(s)	639, 755, 1041
MvnI	CGCG	4 cut(s)	261, 416, 441, 455
MwoI	GCNNNNNNNGC	2 cut(s)	238, 301
NciI	CCSGG	3 cut(s)	90, 108, 160
NdeII	GATC	3 cut(s)	177, 682, 1045
NlaIII	CATG	3 cut(s)	497, 741, 953
NlaIV	GGNNCC	4 cut(s)	430, 473, 759, 855
NmuCI	GTSAC	3 cut(s)	305, 805, 955
NsiI	ATGCAT	1 cut(s)	411
NspI	RCATGY	1 cut(s)	953
PasI	CCCWGGG	1 cut(s)	754
PciSI	GCTCTTC	1 cut(s)	12
PcsI	WCGNNNNNNNCGW	2 cut(s)	126, 189
PdmI	GAANNNNTTC	3 cut(s)	360, 747, 828
PfeI	GAWTC	2 cut(s)	457, 1085
Pfl23II	CGTACG	1 cut(s)	66
PflFI	GACNNNGTC	1 cut(s)	128
PkrI	GCNGC	6 cut(s)	154, 157, 625, 679, 1028, 1131
PleI	GAGTC	2 cut(s)	952, 988
PpsI	GAGTC	2 cut(s)	952, 988
PpuMI	RGGWCCY	1 cut(s)	853
Psp124BI	GAGCTC	1 cut(s)	615
Psp1406I	AACGTT	1 cut(s)	1218
Psp5II	RGGWCCY	1 cut(s)	853
Psp6I	CCWGG	3 cut(s)	637, 753, 1039
PspGI	CCWGG	3 cut(s)	637, 753, 1039
PspLI	CGTACG	1 cut(s)	66
PspN4I	GGNNCC	4 cut(s)	430, 473, 759, 855
PspPI	GGNCC	9 cut(s)	136, 173, 429, 443, 587, 635, 758, 853, 1073
PspPPI	RGGWCCY	1 cut(s)	853
PsrI	GAACNNNNNNTAC	4 cut(s)	50, 82, 345, 377
PstNI	CAGNNNCTG	1 cut(s)	277
PsyI	GACNNNGTC	1 cut(s)	128
PvuII	CAGCTG	1 cut(s)	1132
RsaI	GTAC	1 cut(s)	68
RsaNI	GTAC	1 cut(s)	67
Rsr2I	CGGWCCG	1 cut(s)	173
RsrII	CGGWCCG	1 cut(s)	173
SacI	GAGCTC	1 cut(s)	615
SapI	GCTCTTC	1 cut(s)	12
SaqAI	TTAA	4 cut(s)	215, 669, 1034, 1139
SatI	GCNGC	6 cut(s)	153, 156, 624, 678, 1027, 1130
Sau3AI	GATC	3 cut(s)	177, 682, 1045
Sau96I	GGNCC	9 cut(s)	136, 173, 429, 443, 587, 635, 758, 853, 1073
SchI	GAGTC	2 cut(s)	953, 988
ScrFI	CCNGG	6 cut(s)	90, 108, 160, 639, 755, 1041
SduI	GDGCHC	4 cut(s)	41, 559, 615, 1083
SfaNI	GCATC	3 cut(s)	418, 829, 1085
SfcI	CTRYAG	1 cut(s)	627
SinI	GGWCC	4 cut(s)	136, 173, 429, 853
SmlI	CTYRAG	1 cut(s)	1033
SmoI	CTYRAG	1 cut(s)	1033
Sse9I	AATT	5 cut(s)	421, 483, 895, 976, 1157
SsiI	CCGC	5 cut(s)	153, 156, 426, 441, 578
SspMI	CTAG	4 cut(s)	102, 602, 723, 821
SstI	GAGCTC	1 cut(s)	615
StyD4I	CCNGG	6 cut(s)	88, 106, 158, 637, 753, 1039
StyI	CCWWGG	3 cut(s)	766, 797, 1051
TaaI	ACNGT	1 cut(s)	173
TaiI	ACGT	4 cut(s)	17, 132, 1115, 1221
TaqI	TCGA	5 cut(s)	24, 499, 509, 538, 615
TasI	AATT	5 cut(s)	421, 483, 895, 976, 1157
TauI	GCSGC	2 cut(s)	155, 158
TfiI	GAWTC	2 cut(s)	457, 1085
Tru1I	TTAA	4 cut(s)	215, 669, 1034, 1139
Tru9I	TTAA	4 cut(s)	215, 669, 1034, 1139
TscAI	CASTG	1 cut(s)	1075
TseFI	GTSAC	3 cut(s)	305, 805, 955
TseI	GCWGC	4 cut(s)	623, 677, 1026, 1129
Tsp45I	GTSAC	3 cut(s)	305, 805, 955
TspDTI	ATGAA	7 cut(s)	10, 191, 327, 687, 722, 897, 1191
TspGWI	ACGGA	3 cut(s)	404, 542, 852
TspRI	CASTG	1 cut(s)	1075
Tth111I	GACNNNGTC	1 cut(s)	128
Vha464I	CTTAAG	1 cut(s)	1033
VpaK11BI	GGWCC	4 cut(s)	136, 173, 429, 853
XapI	RAATTY	1 cut(s)	1157
XbaI	TCTAGA	1 cut(s)	820
XceI	RCATGY	1 cut(s)	953
XmnI	GAANNNNTTC	3 cut(s)	360, 747, 828
XspI	CTAG	4 cut(s)	102, 602, 723, 821
ZraI	GACGTC	1 cut(s)	15
Zsp2I	ATGCAT	1 cut(s)	411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.