MD11G1014100.v1.1

BRCA1-A complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007724
Physical Location & Seq
Reverse (-)
1124392 .. 1125237
846 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1014100.v1.1.491

Sequence Viewer

Length: 846 bp
ATGAAGTGTTTGAGTGATTGGAATAGTAGAGACCCTACACAGCTAATGCTTCTCATTCAAGAACTAAGGGATCAGTACATGTCCTACCAGAAGAAGCGCATTGCAGAAGATGATGACAGGTTGAAGTTTGAAATTAGCACAATTGTTGCTCGTGAGGGCATTGAGATGCAGATGAGTTCCAGAGTTGACAAGCCAGAAGAGGTCAAATTTGCAGTTCCTCTATTAGACATGAATATAAATAAGATGGTGCCTGCATACCCCTGGAGACATCAACAAAAGATATACTTGCAGGTTATATATCCTGTTGAGAAAAAATATGTTTCCACTCCTTCAACACCTCGATTGAAACTAGTGTCTACCTCAGAGTTGAAGACTCTATTTTCCATTGATGAAGTCAAACTTCGTCCGTGGTTGAATGGGATGTGCATGGCTGAATACCTTCCGCATCTTGAAGAATCTCTTGAGAAGCAAGTTTTGGAGGCAGTCTCCTTGGTTGATGTTAGGAGGCGCTTTATCGAGGCATTAGCTCTGCAGTTTGGAAGACCAGTGGAAGCTGATTCGGTCTTATGCAGAAAGGCAACATTCCTTACTGCTTCTGGAGTTTTCACTTTTCTGGTTCATGTTTCGATTTCAACTCAGTTTCCAAAGCAAAAGCCGGCTTTGATGCTTCAAAGTTCACATCATTTCAACCTCCAAGGTGTGCCAATCAAGTCACTTCCTCTAACTGAATTTCCTTGGAGCCCTAGATGGGAAGTACCTCATATGGTCGAGCGAATTTGTGAATATTTGACGGACGAGGCTGTGAACTTCAAGAAGCACTGCAACGAGCCTCCACCGCAGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRE

Protein Analysis

282

Amino Acids

32.76

Weight (kDa)

7.67

Isoelectric Point (pI)

58.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRE PF06113 3 - 229 5.3e-26 Brain and reproductive organ-expressed protein (BRE)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 280
AccB1I GGYRCC 1 cut(s) 247
AccI GTMKAC 1 cut(s) 356
AciI CCGC 2 cut(s) 443, 836
AclWI GGATC 1 cut(s) 78
AcsI RAATTY 3 cut(s) 206, 728, 774
AfaI GTAC 2 cut(s) 77, 756
AfiI CCNNNNNNNGG 1 cut(s) 748
AflIII ACRYGT 1 cut(s) 78
AhlI ACTAGT 1 cut(s) 349
AjnI CCWGG 1 cut(s) 260
AjuI GAANNNNNNNTTGG 2 cut(s) 458, 490
AluBI AGCT 3 cut(s) 43, 527, 554
AluI AGCT 3 cut(s) 43, 527, 554
Alw26I GTCTC 3 cut(s) 24, 259, 490
AlwI GGATC 1 cut(s) 78
ApeKI GCWGC 1 cut(s) 838
ApoI RAATTY 3 cut(s) 206, 728, 774
Asp700I GAANNNNTTC 1 cut(s) 438
AspLEI GCGC 2 cut(s) 99, 510
BanI GGYRCC 1 cut(s) 247
BanII GRGCYC 1 cut(s) 743
BauI CACGAG 1 cut(s) 150
BbsI GAAGAC 2 cut(s) 377, 547
BccI CCATC 2 cut(s) 238, 741
BciT130I CCWGG 1 cut(s) 262
BcoDI GTCTC 3 cut(s) 24, 259, 490
BcuI ACTAGT 1 cut(s) 349
BfaI CTAG 3 cut(s) 350, 744, 844
BfmI CTRYAG 1 cut(s) 530
BfoI RGCGCY 1 cut(s) 511
BfuAI ACCTGC 1 cut(s) 280
BisI GCNGC 1 cut(s) 839
BlsI GCNGC 1 cut(s) 840
Bme1390I CCNGG 1 cut(s) 262
BmiI GGNNCC 2 cut(s) 249, 740
BmrFI CCNGG 1 cut(s) 262
BmsI GCATC 3 cut(s) 156, 454, 654
BpiI GAAGAC 2 cut(s) 377, 547
BplI GAGNNNNNCTC 2 cut(s) 470, 502
BpmI CTGGAG 2 cut(s) 283, 618
BpuEI CTTGAG 1 cut(s) 482
BsaI GGTCTC 1 cut(s) 24
BsaJI CCNNGG 5 cut(s) 260, 407, 489, 694, 734
Bsc4I CCNNNNNNNGG 1 cut(s) 748
Bse118I RCCGGY 1 cut(s) 655
Bse1I ACTGG 1 cut(s) 545
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 262
BseDI CCNNGG 5 cut(s) 260, 407, 489, 694, 734
BseGI GGATG 1 cut(s) 426
BseLI CCNNNNNNNGG 1 cut(s) 748
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 2 cut(s) 375, 650
BseNI ACTGG 1 cut(s) 545
BshNI GGYRCC 1 cut(s) 247
BsiSI CCGG 1 cut(s) 656
BslI CCNNNNNNNGG 1 cut(s) 748
BsmAI GTCTC 3 cut(s) 24, 259, 490
Bso31I GGTCTC 1 cut(s) 24
Bsp1286I GDGCHC 1 cut(s) 743
Bsp143I GATC 1 cut(s) 70
BspACI CCGC 2 cut(s) 443, 836
BspCNI CTCAG 2 cut(s) 374, 649
BspLI GGNNCC 2 cut(s) 249, 740
BspMAI CTGCAG 1 cut(s) 534
BspMI ACCTGC 1 cut(s) 280
BspPI GGATC 1 cut(s) 78
BspT107I GGYRCC 1 cut(s) 247
BspTNI GGTCTC 1 cut(s) 24
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 655
BsrI ACTGG 1 cut(s) 545
BssAI RCCGGY 1 cut(s) 655
BssECI CCNNGG 5 cut(s) 260, 407, 489, 694, 734
BssMI GATC 1 cut(s) 70
BssSI CACGAG 1 cut(s) 150
BssT1I CCWWGG 3 cut(s) 489, 694, 734
Bst2BI CACGAG 1 cut(s) 150
Bst2UI CCWGG 1 cut(s) 262
Bst6I CTCTTC 1 cut(s) 192
BstC8I GCNNGC 2 cut(s) 252, 657
BstDEI CTNAG 3 cut(s) 65, 361, 636
BstDSI CCRYGG 1 cut(s) 407
BstF5I GGATG 1 cut(s) 426
BstH2I RGCGCY 1 cut(s) 511
BstHHI GCGC 2 cut(s) 99, 510
BstKTI GATC 1 cut(s) 73
BstMAI GTCTC 3 cut(s) 24, 259, 490
BstMBI GATC 1 cut(s) 70
BstMWI GCNNNNNNNGC 1 cut(s) 835
BstNI CCWGG 1 cut(s) 262
BstNSI RCATGY 1 cut(s) 82
BstSCI CCNGG 1 cut(s) 260
BstSFI CTRYAG 1 cut(s) 530
BstV2I GAAGAC 2 cut(s) 377, 547
BtgI CCRYGG 1 cut(s) 407
BtsCI GGATG 1 cut(s) 426
BtsI GCAGTG 1 cut(s) 817
BtsIMutI CAGTG 2 cut(s) 552, 817
BveI ACCTGC 1 cut(s) 280
Cac8I GCNNGC 2 cut(s) 252, 657
CfoI GCGC 2 cut(s) 99, 510
Cfr10I RCCGGY 1 cut(s) 655
Csp6I GTAC 2 cut(s) 76, 755
CviAII CATG 4 cut(s) 79, 229, 427, 620
CviQI GTAC 2 cut(s) 76, 755
DdeI CTNAG 3 cut(s) 65, 361, 636
DpnI GATC 1 cut(s) 72
DpnII GATC 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
Eco130I CCWWGG 3 cut(s) 489, 694, 734
Eco24I GRGCYC 1 cut(s) 743
Eco31I GGTCTC 1 cut(s) 24
EcoRII CCWGG 1 cut(s) 260
EcoT14I CCWWGG 3 cut(s) 489, 694, 734
EcoT38I GRGCYC 1 cut(s) 743
ErhI CCWWGG 3 cut(s) 489, 694, 734
FaeI CATG 4 cut(s) 82, 232, 430, 623
FalI AAGNNNNNCTT 6 cut(s) 269, 301, 384, 416, 444, 476
FatI CATG 4 cut(s) 78, 228, 426, 619
FauNDI CATATG 1 cut(s) 762
FblI GTMKAC 1 cut(s) 356
Fnu4HI GCNGC 1 cut(s) 839
FokI GGATG 1 cut(s) 433
FriOI GRGCYC 1 cut(s) 743
Fsp4HI GCNGC 1 cut(s) 839
FspBI CTAG 3 cut(s) 350, 744, 844
GlaI GCGC 2 cut(s) 98, 509
GluI GCNGC 1 cut(s) 839
GsuI CTGGAG 2 cut(s) 283, 618
HaeII RGCGCY 1 cut(s) 511
HapII CCGG 1 cut(s) 656
HhaI GCGC 2 cut(s) 99, 510
Hin1II CATG 4 cut(s) 82, 232, 430, 623
Hin6I GCGC 2 cut(s) 97, 508
HinP1I GCGC 2 cut(s) 97, 508
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 3 cut(s) 373, 455, 557
HpaII CCGG 1 cut(s) 656
Hpy166II GTNNAC 4 cut(s) 187, 357, 677, 805
Hpy188I TCNGA 1 cut(s) 364
Hpy188III TCNNGA 7 cut(s) 59, 152, 180, 449, 461, 597, 811
Hpy8I GTNNAC 4 cut(s) 187, 357, 677, 805
HpyAV CCTTC 2 cut(s) 339, 449
HpyCH4V TGCA 9 cut(s) 104, 169, 212, 254, 289, 426, 532, 570, 822
HpyF10VI GCNNNNNNNGC 1 cut(s) 835
HpyF3I CTNAG 3 cut(s) 65, 361, 636
Hsp92II CATG 4 cut(s) 82, 232, 430, 623
HspAI GCGC 2 cut(s) 97, 508
KroI GCCGGC 1 cut(s) 655
KroNI GCCGGC 1 cut(s) 657
Kzo9I GATC 1 cut(s) 70
LmnI GCTCC 1 cut(s) 738
LweI GCATC 3 cut(s) 156, 454, 654
MaeI CTAG 3 cut(s) 350, 744, 844
MaeIII GTNAC 1 cut(s) 711
MalI GATC 1 cut(s) 72
MboI GATC 1 cut(s) 70
MboII GAAGA 6 cut(s) 103, 119, 209, 382, 464, 552
MfeI CAATTG 1 cut(s) 141
MhlI GDGCHC 1 cut(s) 743
MluCI AATT 5 cut(s) 132, 141, 206, 728, 774
MlyI GAGTC 1 cut(s) 367
MroNI GCCGGC 1 cut(s) 655
MroXI GAANNNNTTC 1 cut(s) 438
MslI CAYNNNNRTG 1 cut(s) 164
MspI CCGG 1 cut(s) 656
MspR9I CCNGG 1 cut(s) 262
MunI CAATTG 1 cut(s) 141
MvaI CCWGG 1 cut(s) 262
MwoI GCNNNNNNNGC 1 cut(s) 835
NaeI GCCGGC 1 cut(s) 657
NdeI CATATG 1 cut(s) 762
NdeII GATC 1 cut(s) 70
NgoMIV GCCGGC 1 cut(s) 655
NlaIII CATG 4 cut(s) 82, 232, 430, 623
NlaIV GGNNCC 2 cut(s) 249, 740
NmuCI GTSAC 1 cut(s) 711
NspI RCATGY 1 cut(s) 82
PciI ACATGT 1 cut(s) 78
PdiI GCCGGC 1 cut(s) 657
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 2 cut(s) 455, 557
PkrI GCNGC 1 cut(s) 840
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
PscI ACATGT 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 260
PspGI CCWGG 1 cut(s) 260
PspN4I GGNNCC 2 cut(s) 249, 740
PstI CTGCAG 1 cut(s) 534
RsaI GTAC 2 cut(s) 77, 756
RsaNI GTAC 2 cut(s) 76, 755
RseI CAYNNNNRTG 1 cut(s) 164
SatI GCNGC 1 cut(s) 839
Sau3AI GATC 1 cut(s) 70
SchI GAGTC 1 cut(s) 367
ScrFI CCNGG 1 cut(s) 262
SduI GDGCHC 1 cut(s) 743
SfaNI GCATC 3 cut(s) 156, 454, 654
SfcI CTRYAG 1 cut(s) 530
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 461
SmoI CTYRAG 1 cut(s) 461
SpeI ACTAGT 1 cut(s) 349
Sse9I AATT 5 cut(s) 132, 141, 206, 728, 774
SsiI CCGC 2 cut(s) 443, 836
SspI AATATT 1 cut(s) 785
SspMI CTAG 3 cut(s) 350, 744, 844
StyD4I CCNGG 1 cut(s) 260
StyI CCWWGG 3 cut(s) 489, 694, 734
TaqI TCGA 4 cut(s) 340, 516, 626, 768
TaqII GACCGA 1 cut(s) 550
TasI AATT 5 cut(s) 132, 141, 206, 728, 774
TatI WGTACW 1 cut(s) 75
TfiI GAWTC 2 cut(s) 455, 557
TscAI CASTG 2 cut(s) 552, 824
TseFI GTSAC 1 cut(s) 711
TseI GCWGC 1 cut(s) 838
Tsp45I GTSAC 1 cut(s) 711
TspDTI ATGAA 4 cut(s) 17, 245, 405, 608
TspGWI ACGGA 2 cut(s) 396, 806
TspRI CASTG 2 cut(s) 552, 824
XapI RAATTY 3 cut(s) 206, 728, 774
XceI RCATGY 1 cut(s) 82
XmiI GTMKAC 1 cut(s) 356
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 3 cut(s) 350, 744, 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.