MD15G1011700.v1.1

BRCA1-A complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007724
Physical Location & Seq
Forward (+)
637605 .. 640885
3281 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1011700.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGTCGTCGGAGAGTGTTCCGCCGCTCGTCGCCACCCAGCTCAACTACCTCCTCACTCACTTCCCTCTCGTCGTCAAGATTGAGAACAAGTGGTCTGGCAGCAAGTACTACCCCGGATTTCTCGACCGGTTCACCTTAGTCATTTCCTACTGTCTAGACGTTATCAAGTGGGATGTTATATTTGATTCGGAGTCTCCATTCTCTGCACCGGATGTTATATTCGGACCTGATGATGAGAGATTTCAGCAGTTTCTTGTATCGCCCGATGAGGGAGAAGGGGACTCAAAATTTCACATGAAGTGTTTGAGTGATTGGAATAGCAGAGACCCTACACAGCTAATGCTTCTCATTCAAGAACTAAGGGATCAGTACATGTCCTACCAGAAGAAGCGCGTTGCAGAAGTTGATGATGACAGGTTGAAGTTTGAAATTAGCACAATTGTTGCTCGTGAGGGCATTGAGATGCAGATGAGTTCCGGAGTTGACAAGCCAGAGGAGGTCAAATTTGCAGTTCCTCTATTAGACATGAACATAAATAAGATGGTGCCTGCATACCCCTGGAGACATCAACAAAAGATATACTTGCAGGTTATATATCCTGTTGAGAAAAAATATGTTTCCACTCCTTCAGCACCTCGATTGAAACTAGTGTCTACCTCAGAGTTGAAGACTCTATTTTCCATTGATGAAGTCAAACTTCGTCCGTGGTTGAATGGGATGTGCATGGCTGAATACCTTCCGCATCTTGAAGAATCTCTTGAGAAGCAGGTTTTGGAGGCAGTCTCCTTGGTTGATGTTAGGAGGCGCTTTATTGAGGCATTAGCTCTGCAGTTTGGAAGACCAGTGGAAGCTGATTCGGTCTTATGCAGAAAGGCAACATTCCTTACTGCTTCTGGAGTTTTCACTTTTCTGGTTCATGTTTCGATTTCAACTCAGTTTCCAAAGCAAAAGCCGGCTTTGATGCTTCAAAGTTCACAGCATTTCAACCTCCAAGGTGTGCCAATCAAGTCGCTTCCTCTAACTGAATTTCCTTGGAGCCCTAGATGGGAAGTACCTCATATGGCCGAGCGAATTTGTGAATATTTGACGGACGAGGCTGTGAACTTCAAGAAGCACTGCAACGAGCCTCCACCGCAGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRE

Protein Analysis

381

Amino Acids

43.86

Weight (kDa)

5.68

Isoelectric Point (pI)

57.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRE PF06113 25 - 328 3e-38 Brain and reproductive organ-expressed protein (BRE)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 577, 757
AccB1I GGYRCC 1 cut(s) 544
AccBSI CCGCTC 1 cut(s) 25
AccI GTMKAC 1 cut(s) 653
AccII CGCG 1 cut(s) 393
AccIII TCCGGA 1 cut(s) 476
AciI CCGC 4 cut(s) 20, 23, 740, 1133
AclWI GGATC 1 cut(s) 372
AcoI YGGCCR 1 cut(s) 1062
AcsI RAATTY 4 cut(s) 287, 503, 1025, 1071
AcuI CTGAAG 1 cut(s) 612
AfaI GTAC 3 cut(s) 107, 371, 1053
AfiI CCNNNNNNNGG 2 cut(s) 269, 1045
AflIII ACRYGT 1 cut(s) 372
AgeI ACCGGT 1 cut(s) 126
AhlI ACTAGT 1 cut(s) 646
AjnI CCWGG 1 cut(s) 557
AjuI GAANNNNNNNTTGG 2 cut(s) 755, 787
AluBI AGCT 4 cut(s) 40, 337, 824, 851
AluI AGCT 4 cut(s) 40, 337, 824, 851
Alw26I GTCTC 4 cut(s) 198, 318, 556, 787
AlwI GGATC 1 cut(s) 372
Aor13HI TCCGGA 1 cut(s) 476
AoxI GGCC 1 cut(s) 1062
ApeKI GCWGC 2 cut(s) 99, 1135
ApoI RAATTY 4 cut(s) 287, 503, 1025, 1071
AsiGI ACCGGT 1 cut(s) 126
Asp700I GAANNNNTTC 1 cut(s) 735
AspLEI GCGC 2 cut(s) 393, 807
AspS9I GGNCC 1 cut(s) 224
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 1 cut(s) 124
AvaII GGWCC 1 cut(s) 224
BanI GGYRCC 1 cut(s) 544
BanII GRGCYC 1 cut(s) 1040
BauI CACGAG 1 cut(s) 447
BbsI GAAGAC 2 cut(s) 674, 844
BbvI GCAGC 1 cut(s) 111
BccI CCATC 2 cut(s) 535, 1038
BciT130I CCWGG 1 cut(s) 559
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 4 cut(s) 198, 318, 556, 787
BcuI ACTAGT 1 cut(s) 646
BfaI CTAG 4 cut(s) 155, 647, 1041, 1141
BfmI CTRYAG 1 cut(s) 827
BfoI RGCGCY 1 cut(s) 808
BfuAI ACCTGC 2 cut(s) 577, 757
BisI GCNGC 3 cut(s) 23, 100, 1136
BlsI GCNGC 3 cut(s) 24, 101, 1137
BmcAI AGTACT 1 cut(s) 107
Bme1390I CCNGG 2 cut(s) 114, 559
Bme18I GGWCC 1 cut(s) 224
BmgT120I GGNCC 1 cut(s) 224
BmiI GGNNCC 2 cut(s) 546, 1037
BmrFI CCNGG 2 cut(s) 114, 559
BmsI GCATC 3 cut(s) 453, 751, 951
BpiI GAAGAC 2 cut(s) 674, 844
BplI GAGNNNNNCTC 2 cut(s) 767, 799
BpmI CTGGAG 2 cut(s) 580, 915
BpuEI CTTGAG 1 cut(s) 779
BpuMI CCSGG 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 318
BsaJI CCNNGG 6 cut(s) 112, 557, 704, 786, 991, 1031
BsaWI WCCGGW 3 cut(s) 126, 208, 476
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 1045
Bse118I RCCGGY 2 cut(s) 126, 952
Bse1I ACTGG 1 cut(s) 842
BseAI TCCGGA 1 cut(s) 476
BseBI CCWGG 1 cut(s) 559
BseDI CCNNGG 6 cut(s) 112, 557, 704, 786, 991, 1031
BseGI GGATG 3 cut(s) 178, 217, 723
BseLI CCNNNNNNNGG 2 cut(s) 269, 1045
BseMII CTCAG 2 cut(s) 672, 947
BseNI ACTGG 1 cut(s) 842
BseRI GAGGAG 2 cut(s) 41, 509
BseXI GCAGC 1 cut(s) 111
BseYI CCCAGC 1 cut(s) 36
BsgI GTGCAG 1 cut(s) 189
Bsh1236I CGCG 1 cut(s) 393
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 1 cut(s) 1064
BshNI GGYRCC 1 cut(s) 544
BshTI ACCGGT 1 cut(s) 126
BsiEI CGRYCG 1 cut(s) 127
BsiSI CCGG 5 cut(s) 114, 127, 209, 477, 953
BslFI GGGAC 1 cut(s) 293
BslI CCNNNNNNNGG 2 cut(s) 269, 1045
BsmAI GTCTC 4 cut(s) 198, 318, 556, 787
BsmFI GGGAC 1 cut(s) 293
BsnI GGCC 1 cut(s) 1064
Bso31I GGTCTC 1 cut(s) 318
Bsp1286I GDGCHC 1 cut(s) 1040
Bsp13I TCCGGA 1 cut(s) 476
Bsp143I GATC 1 cut(s) 364
BspACI CCGC 4 cut(s) 20, 23, 740, 1133
BspANI GGCC 1 cut(s) 1064
BspCNI CTCAG 2 cut(s) 671, 946
BspEI TCCGGA 1 cut(s) 476
BspFNI CGCG 1 cut(s) 393
BspLI GGNNCC 2 cut(s) 546, 1037
BspMAI CTGCAG 1 cut(s) 831
BspMI ACCTGC 2 cut(s) 577, 757
BspPI GGATC 1 cut(s) 372
BspT107I GGYRCC 1 cut(s) 544
BspTNI GGTCTC 1 cut(s) 318
BsrBI CCGCTC 1 cut(s) 25
BsrFI RCCGGY 2 cut(s) 126, 952
BsrI ACTGG 1 cut(s) 842
BssAI RCCGGY 2 cut(s) 126, 952
BssECI CCNNGG 6 cut(s) 112, 557, 704, 786, 991, 1031
BssMI GATC 1 cut(s) 364
BssSI CACGAG 1 cut(s) 447
BssT1I CCWWGG 3 cut(s) 786, 991, 1031
Bst2BI CACGAG 1 cut(s) 447
Bst2UI CCWGG 1 cut(s) 559
Bst4CI ACNGT 1 cut(s) 152
BstC8I GCNNGC 2 cut(s) 549, 954
BstDEI CTNAG 4 cut(s) 136, 359, 658, 933
BstDSI CCRYGG 1 cut(s) 704
BstF5I GGATG 3 cut(s) 178, 217, 723
BstFNI CGCG 1 cut(s) 393
BstH2I RGCGCY 1 cut(s) 808
BstHHI GCGC 2 cut(s) 393, 807
BstKTI GATC 1 cut(s) 367
BstMAI GTCTC 4 cut(s) 198, 318, 556, 787
BstMBI GATC 1 cut(s) 364
BstMCI CGRYCG 1 cut(s) 127
BstMWI GCNNNNNNNGC 1 cut(s) 1132
BstNI CCWGG 1 cut(s) 559
BstNSI RCATGY 1 cut(s) 376
BstSCI CCNGG 2 cut(s) 112, 557
BstSFI CTRYAG 1 cut(s) 827
BstUI CGCG 1 cut(s) 393
BstV1I GCAGC 1 cut(s) 111
BstV2I GAAGAC 2 cut(s) 674, 844
BsuRI GGCC 1 cut(s) 1064
BtgI CCRYGG 1 cut(s) 704
BtsCI GGATG 3 cut(s) 178, 217, 723
BtsI GCAGTG 1 cut(s) 1114
BtsIMutI CAGTG 2 cut(s) 849, 1114
BveI ACCTGC 2 cut(s) 577, 757
Cac8I GCNNGC 2 cut(s) 549, 954
CfoI GCGC 2 cut(s) 393, 807
Cfr10I RCCGGY 2 cut(s) 126, 952
Cfr13I GGNCC 1 cut(s) 224
Csp6I GTAC 3 cut(s) 106, 370, 1052
CspAI ACCGGT 1 cut(s) 126
CviAII CATG 5 cut(s) 295, 373, 526, 724, 917
CviQI GTAC 3 cut(s) 106, 370, 1052
DdeI CTNAG 4 cut(s) 136, 359, 658, 933
DpnI GATC 1 cut(s) 366
DpnII GATC 1 cut(s) 364
EaeI YGGCCR 1 cut(s) 1062
EciI GGCGGA 1 cut(s) 9
Eco130I CCWWGG 3 cut(s) 786, 991, 1031
Eco24I GRGCYC 1 cut(s) 1040
Eco31I GGTCTC 1 cut(s) 318
Eco47I GGWCC 1 cut(s) 224
Eco57I CTGAAG 1 cut(s) 612
EcoRII CCWGG 1 cut(s) 557
EcoT14I CCWWGG 3 cut(s) 786, 991, 1031
EcoT38I GRGCYC 1 cut(s) 1040
ErhI CCWWGG 3 cut(s) 786, 991, 1031
FaeI CATG 5 cut(s) 298, 376, 529, 727, 920
FalI AAGNNNNNCTT 6 cut(s) 566, 598, 681, 713, 741, 773
FaqI GGGAC 1 cut(s) 293
FatI CATG 5 cut(s) 294, 372, 525, 723, 916
FauNDI CATATG 1 cut(s) 1059
FblI GTMKAC 1 cut(s) 653
Fnu4HI GCNGC 3 cut(s) 23, 100, 1136
FokI GGATG 3 cut(s) 185, 224, 730
FriOI GRGCYC 1 cut(s) 1040
Fsp4HI GCNGC 3 cut(s) 23, 100, 1136
FspBI CTAG 4 cut(s) 155, 647, 1041, 1141
GlaI GCGC 2 cut(s) 392, 806
GluI GCNGC 3 cut(s) 23, 100, 1136
GsaI CCCAGC 1 cut(s) 40
GsuI CTGGAG 2 cut(s) 580, 915
HaeII RGCGCY 1 cut(s) 808
HaeIII GGCC 1 cut(s) 1064
HapII CCGG 5 cut(s) 114, 127, 209, 477, 953
HhaI GCGC 2 cut(s) 393, 807
Hin1II CATG 5 cut(s) 298, 376, 529, 727, 920
Hin6I GCGC 2 cut(s) 391, 805
HinP1I GCGC 2 cut(s) 391, 805
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HinfI GANTC 6 cut(s) 185, 191, 281, 670, 752, 854
HpaII CCGG 5 cut(s) 114, 127, 209, 477, 953
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 5 cut(s) 132, 484, 654, 974, 1102
Hpy188I TCNGA 4 cut(s) 10, 190, 224, 661
Hpy8I GTNNAC 5 cut(s) 132, 484, 654, 974, 1102
Hpy99I CGWCG 3 cut(s) 10, 32, 74
HpyAV CCTTC 3 cut(s) 269, 636, 746
HpyCH4III ACNGT 1 cut(s) 152
HpyCH4IV ACGT 1 cut(s) 159
HpyF10VI GCNNNNNNNGC 1 cut(s) 1132
HpyF3I CTNAG 4 cut(s) 136, 359, 658, 933
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 5 cut(s) 298, 376, 529, 727, 920
HspAI GCGC 2 cut(s) 391, 805
Kpn2I TCCGGA 1 cut(s) 476
KroI GCCGGC 1 cut(s) 952
KroNI GCCGGC 1 cut(s) 954
Kzo9I GATC 1 cut(s) 364
LmnI GCTCC 1 cut(s) 1035
Lsp1109I GCAGC 1 cut(s) 111
LweI GCATC 3 cut(s) 453, 751, 951
MaeI CTAG 4 cut(s) 155, 647, 1041, 1141
MaeII ACGT 1 cut(s) 159
MalI GATC 1 cut(s) 366
MbiI CCGCTC 1 cut(s) 25
MboI GATC 1 cut(s) 364
MboII GAAGA 4 cut(s) 397, 679, 761, 849
MfeI CAATTG 1 cut(s) 438
MhlI GDGCHC 1 cut(s) 1040
MluCI AATT 6 cut(s) 287, 429, 438, 503, 1025, 1071
MlyI GAGTC 3 cut(s) 200, 275, 664
MroI TCCGGA 1 cut(s) 476
MroNI GCCGGC 1 cut(s) 952
MroXI GAANNNNTTC 1 cut(s) 735
MslI CAYNNNNRTG 1 cut(s) 461
MspI CCGG 5 cut(s) 114, 127, 209, 477, 953
MspR9I CCNGG 2 cut(s) 114, 559
MunI CAATTG 1 cut(s) 438
MvaI CCWGG 1 cut(s) 559
MvnI CGCG 1 cut(s) 393
MwoI GCNNNNNNNGC 1 cut(s) 1132
NaeI GCCGGC 1 cut(s) 954
NciI CCSGG 1 cut(s) 114
NdeI CATATG 1 cut(s) 1059
NdeII GATC 1 cut(s) 364
NgoMIV GCCGGC 1 cut(s) 952
NlaIII CATG 5 cut(s) 298, 376, 529, 727, 920
NlaIV GGNNCC 2 cut(s) 546, 1037
NmeAIII GCCGAG 1 cut(s) 1090
NspI RCATGY 1 cut(s) 376
PciI ACATGT 1 cut(s) 372
PdiI GCCGGC 1 cut(s) 954
PdmI GAANNNNTTC 1 cut(s) 735
PfeI GAWTC 3 cut(s) 185, 752, 854
PinAI ACCGGT 1 cut(s) 126
PkrI GCNGC 3 cut(s) 24, 101, 1137
PleI GAGTC 3 cut(s) 199, 275, 664
PpsI GAGTC 3 cut(s) 199, 275, 664
PscI ACATGT 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 557
PspFI CCCAGC 1 cut(s) 36
PspGI CCWGG 1 cut(s) 557
PspN4I GGNNCC 2 cut(s) 546, 1037
PspPI GGNCC 1 cut(s) 224
PstI CTGCAG 1 cut(s) 831
RsaI GTAC 3 cut(s) 107, 371, 1053
RsaNI GTAC 3 cut(s) 106, 370, 1052
RseI CAYNNNNRTG 1 cut(s) 461
SatI GCNGC 3 cut(s) 23, 100, 1136
Sau3AI GATC 1 cut(s) 364
Sau96I GGNCC 1 cut(s) 224
ScaI AGTACT 1 cut(s) 107
SchI GAGTC 3 cut(s) 200, 275, 664
ScrFI CCNGG 2 cut(s) 114, 559
SduI GDGCHC 1 cut(s) 1040
SfaNI GCATC 3 cut(s) 453, 751, 951
SfcI CTRYAG 1 cut(s) 827
SinI GGWCC 1 cut(s) 224
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 1 cut(s) 758
SmoI CTYRAG 1 cut(s) 758
SpeI ACTAGT 1 cut(s) 646
Sse9I AATT 6 cut(s) 287, 429, 438, 503, 1025, 1071
SsiI CCGC 4 cut(s) 20, 23, 740, 1133
SspI AATATT 1 cut(s) 1082
SspMI CTAG 4 cut(s) 155, 647, 1041, 1141
StyD4I CCNGG 2 cut(s) 112, 557
StyI CCWWGG 3 cut(s) 786, 991, 1031
TaaI ACNGT 1 cut(s) 152
TaiI ACGT 1 cut(s) 162
TaqI TCGA 3 cut(s) 123, 637, 923
TaqII GACCGA 1 cut(s) 847
TasI AATT 6 cut(s) 287, 429, 438, 503, 1025, 1071
TatI WGTACW 2 cut(s) 105, 369
TauI GCSGC 1 cut(s) 25
TfiI GAWTC 3 cut(s) 185, 752, 854
TscAI CASTG 2 cut(s) 849, 1121
TseI GCWGC 2 cut(s) 99, 1135
TspDTI ATGAA 4 cut(s) 311, 542, 702, 905
TspGWI ACGGA 2 cut(s) 693, 1103
TspRI CASTG 2 cut(s) 849, 1121
VpaK11BI GGWCC 1 cut(s) 224
XapI RAATTY 4 cut(s) 287, 503, 1025, 1071
XbaI TCTAGA 1 cut(s) 154
XceI RCATGY 1 cut(s) 376
XmiI GTMKAC 1 cut(s) 653
XmnI GAANNNNTTC 1 cut(s) 735
XspI CTAG 4 cut(s) 155, 647, 1041, 1141
ZrmI AGTACT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.