RchiOBHm_Chr4g0398171

BRCA1-A complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0007724
Physical Location & Seq
Reverse (-)
14594704 .. 14595488
785 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37036

Sequence Viewer

Length: 477 bp
ATGGTTAGGAAAGTACTCTGTCTAGACTTTATCAAATGTGACTTTATCAAAGTTCTTGATCGGTTTACCTTGGCCATTTCCTACTGTCTAGACTTTATCAAATGGGATGTTATATTTGATGCGGAGTATCCGATGTCTGCACCCGATATTATATTCGGACCTGAAGATGAAAGTTTTCAGCAGTTTCTTGTATTGCATGGTGAAGATCCGAAGTCTCCCATGAACCGTTTGGGTGGTTGGGATAGCAGAGACCCTACGCAGCTCATGCTTCTCGTTCAAGAACTGAGAGATCAATACATGGCCTACCGGATGAAGCGTGTTGGAGAGGTTCAAGATGACCGCGTGAAATTTGAAATTAGCACAATTGCTTTTCGGGAGGTAATGTTTCAATTCAATATTGTTTGTCTTGCAGACTGTGAGAACTTTAAATTTATATACCGAGTGCGAGGGGATGCACTAAGAAGTCTTCTTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03440 map03440
Pfam Domains
Protein Families
BRE

Protein Analysis

158

Amino Acids

18.67

Weight (kDa)

4.94

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRE PF06113 18 - 133 9.3e-19 Brain and reproductive organ-expressed protein (BRE)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 342
AciI CCGC 2 cut(s) 122, 340
AclWI GGATC 1 cut(s) 200
AcoI YGGCCR 1 cut(s) 72
AcsI RAATTY 2 cut(s) 347, 428
AcuI CTGAAG 1 cut(s) 183
AfaI GTAC 1 cut(s) 15
AgsI TTSAA 5 cut(s) 278, 332, 353, 389, 394
AluBI AGCT 1 cut(s) 262
AluI AGCT 1 cut(s) 262
Alw26I GTCTC 2 cut(s) 219, 243
AlwI GGATC 1 cut(s) 200
AoxI GGCC 2 cut(s) 72, 300
ApeKI GCWGC 1 cut(s) 259
ApoI RAATTY 2 cut(s) 347, 428
Asp700I GAANNNNTTC 1 cut(s) 174
AspS9I GGNCC 1 cut(s) 158
AsuHPI GGTGA 1 cut(s) 212
AvaII GGWCC 1 cut(s) 158
BalI TGGCCA 1 cut(s) 74
BbsI GAAGAC 1 cut(s) 458
BbvI GCAGC 1 cut(s) 271
BciVI GTATCC 1 cut(s) 138
BcoDI GTCTC 2 cut(s) 219, 243
BfaI CTAG 2 cut(s) 23, 89
BfuI GTATCC 1 cut(s) 138
BisI GCNGC 1 cut(s) 260
BlsI GCNGC 1 cut(s) 261
BmcAI AGTACT 1 cut(s) 15
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 158
BmsI GCATC 2 cut(s) 109, 442
BpiI GAAGAC 1 cut(s) 458
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 1 cut(s) 69
BsaWI WCCGGW 1 cut(s) 306
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
BseDI CCNNGG 1 cut(s) 69
BseGI GGATG 3 cut(s) 112, 315, 457
BseMII CTCAG 1 cut(s) 275
BseXI GCAGC 1 cut(s) 271
BsgI GTGCAG 1 cut(s) 123
Bsh1236I CGCG 1 cut(s) 342
BshFI GGCC 2 cut(s) 74, 302
BsiSI CCGG 1 cut(s) 307
BsmAI GTCTC 2 cut(s) 219, 243
BsnI GGCC 2 cut(s) 74, 302
Bso31I GGTCTC 1 cut(s) 243
Bsp143I GATC 3 cut(s) 58, 205, 289
BspACI CCGC 2 cut(s) 122, 340
BspANI GGCC 2 cut(s) 74, 302
BspCNI CTCAG 1 cut(s) 276
BspFNI CGCG 1 cut(s) 342
BspPI GGATC 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 243
BssECI CCNNGG 1 cut(s) 69
BssMI GATC 3 cut(s) 58, 205, 289
BssT1I CCWWGG 1 cut(s) 69
Bst4CI ACNGT 3 cut(s) 86, 227, 416
BstAPI GCANNNNNTGC 1 cut(s) 265
BstDEI CTNAG 2 cut(s) 284, 458
BstF5I GGATG 3 cut(s) 112, 315, 457
BstFNI CGCG 1 cut(s) 342
BstKTI GATC 3 cut(s) 61, 208, 292
BstMAI GTCTC 2 cut(s) 219, 243
BstMBI GATC 3 cut(s) 58, 205, 289
BstMWI GCNNNNNNNGC 1 cut(s) 265
BstUI CGCG 1 cut(s) 342
BstV1I GCAGC 1 cut(s) 271
BstV2I GAAGAC 1 cut(s) 458
BstX2I RGATCY 1 cut(s) 205
BstYI RGATCY 1 cut(s) 205
BsuI GTATCC 1 cut(s) 138
BsuRI GGCC 2 cut(s) 74, 302
BtsCI GGATG 3 cut(s) 112, 315, 457
Cfr13I GGNCC 1 cut(s) 158
Csp6I GTAC 1 cut(s) 14
CviAII CATG 4 cut(s) 197, 220, 265, 298
CviJI RGCY 3 cut(s) 74, 262, 302
CviKI_1 RGCY 3 cut(s) 74, 262, 302
CviQI GTAC 1 cut(s) 14
DdeI CTNAG 2 cut(s) 284, 458
DpnI GATC 3 cut(s) 60, 207, 291
DpnII GATC 3 cut(s) 58, 205, 289
DraI TTTAAA 1 cut(s) 427
EaeI YGGCCR 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 69
Eco31I GGTCTC 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 158
Eco57I CTGAAG 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 4 cut(s) 200, 223, 268, 301
FaiI YATR 8 cut(s) 113, 152, 198, 221, 266, 299, 434, 436
FatI CATG 4 cut(s) 196, 219, 264, 297
Fnu4HI GCNGC 1 cut(s) 260
FokI GGATG 3 cut(s) 119, 322, 464
Fsp4HI GCNGC 1 cut(s) 260
FspBI CTAG 2 cut(s) 23, 89
GluI GCNGC 1 cut(s) 260
HaeIII GGCC 2 cut(s) 74, 302
HapII CCGG 1 cut(s) 307
Hin1II CATG 4 cut(s) 200, 223, 268, 301
HpaII CCGG 1 cut(s) 307
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 1 cut(s) 66
Hpy188I TCNGA 3 cut(s) 132, 158, 210
Hpy188III TCNNGA 6 cut(s) 23, 56, 89, 278, 332, 374
Hpy8I GTNNAC 1 cut(s) 66
HpyCH4III ACNGT 3 cut(s) 86, 227, 416
HpyCH4V TGCA 4 cut(s) 140, 196, 410, 455
HpyF10VI GCNNNNNNNGC 1 cut(s) 265
HpyF3I CTNAG 2 cut(s) 284, 458
Hsp92II CATG 4 cut(s) 200, 223, 268, 301
Kzo9I GATC 3 cut(s) 58, 205, 289
LpnPI CCDG 2 cut(s) 174, 320
Lsp1109I GCAGC 1 cut(s) 271
LweI GCATC 2 cut(s) 109, 442
MaeI CTAG 2 cut(s) 23, 89
MaeIII GTNAC 1 cut(s) 38
MalI GATC 3 cut(s) 60, 207, 291
MboI GATC 3 cut(s) 58, 205, 289
MboII GAAGA 3 cut(s) 176, 215, 458
MfeI CAATTG 1 cut(s) 363
MflI RGATCY 1 cut(s) 205
MlsI TGGCCA 1 cut(s) 74
MluCI AATT 5 cut(s) 347, 354, 363, 389, 428
MluNI TGGCCA 1 cut(s) 74
MmeI TCCRAC 1 cut(s) 301
MnlI CCTC 3 cut(s) 319, 370, 440
Mox20I TGGCCA 1 cut(s) 74
MroXI GAANNNNTTC 1 cut(s) 174
MscI TGGCCA 1 cut(s) 74
MseI TTAA 1 cut(s) 426
Msp20I TGGCCA 1 cut(s) 74
MspI CCGG 1 cut(s) 307
MunI CAATTG 1 cut(s) 363
MvnI CGCG 1 cut(s) 342
MwoI GCNNNNNNNGC 1 cut(s) 265
NdeII GATC 3 cut(s) 58, 205, 289
NlaIII CATG 4 cut(s) 200, 223, 268, 301
NmuCI GTSAC 1 cut(s) 38
PdmI GAANNNNTTC 1 cut(s) 174
PkrI GCNGC 1 cut(s) 261
PspPI GGNCC 1 cut(s) 158
PsuI RGATCY 1 cut(s) 205
RsaI GTAC 1 cut(s) 15
RsaNI GTAC 1 cut(s) 14
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 1 cut(s) 260
Sau3AI GATC 3 cut(s) 58, 205, 289
Sau96I GGNCC 1 cut(s) 158
ScaI AGTACT 1 cut(s) 15
SetI ASST 5 cut(s) 71, 163, 264, 330, 381
SfaNI GCATC 2 cut(s) 109, 442
SinI GGWCC 1 cut(s) 158
Sse9I AATT 5 cut(s) 347, 354, 363, 389, 428
SsiI CCGC 2 cut(s) 122, 340
SspI AATATT 1 cut(s) 397
SspMI CTAG 2 cut(s) 23, 89
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 3 cut(s) 86, 227, 416
TasI AATT 5 cut(s) 347, 354, 363, 389, 428
TatI WGTACW 1 cut(s) 13
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TseFI GTSAC 1 cut(s) 38
TseI GCWGC 1 cut(s) 259
Tsp45I GTSAC 1 cut(s) 38
TspDTI ATGAA 3 cut(s) 183, 236, 326
VpaK11BI GGWCC 1 cut(s) 158
XapI RAATTY 2 cut(s) 347, 428
XbaI TCTAGA 2 cut(s) 22, 88
XcmI CCANNNNNNNNNTGG 1 cut(s) 226
XmnI GAANNNNTTC 1 cut(s) 174
XspI CTAG 2 cut(s) 23, 89
ZrmI AGTACT 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.