Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005429

Physical Location & Seq

Genomic Coordinates Forward (+)

14450214 .. 14461048

Gene Structure 10835 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1151700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2889 bp

ATGGCCCCGATTCCGCCCGATAACGGCGTCGAAGGCGACGACGAGATAGAGGAAGAGGGCGAGGAGGAAGAAGAAGAGGAAGAGGAGGAAGAAGAAGAAGAAGAAGAAGAAGAGGAAGAGGAGCCGAGGCTCAAGTACCAGCGAATGGGAGGCAGCATTCCCACACTGTTAACGAGCGATGTGGCGTCGTGCATAGCCGTCGCAGAACGAATGATCGCGCTCGGGACTCACGGCGGCACCGTTCATATTCTCGATTTTCTCGGGAATCAGGTTAAGGAATTCCCTGCTCATACTGCTGCAGTGAATGATCTTAGCTTCGACATAGAAGGTGAATTTATAGGAAGCTGTTCAGATGATGGGTCTGTTGTAATAAGCAGTCTTTTCACTGATGAGAAAATGAGGTTTGAGTATCATCGCCCAATGAAGGCTATTGCCCTGGATCCAGATTATGTAAAAAAATCATCTAGAAGATTTGTAGCTGGTGGTTTAGCTGGTCATTTATATTTTAATACAAAAAGATGGCTGGGCTTTAAAGATCAGGTTTTGCACTCTGGTGAAGGTCCTATACATGCAGTGAAGTGGAGAACGAGTCTCATTGCTTGGGCTAATGATGCAGGTGTGAAAGTGTACAATACTGCAAATGATCAACGTATAACATTCATTGAAAGGCCACGAGGAAGCCCACGTCCTGAGCTTTTGCTTCCTCACTTATTTTGGCAGGATGACTCATTGTTGGTCATTGGCTGGGGAACATCAATCAAAATCACATCGATAAAAACAAATCAGTCTAGAGCCACCAATGGGACATATAACCATGTCCCATTGTCTGGTATGAACCAAGTGGATATTGTGGCATCCTTTCAGACCAGCTATTTCATTTCTGGGATTGCCCCTTTTGGTGATACTTTGGTCGTTCTAGCCTATATCCCTGTTGAAGAAGATGGGGAGAAGGAATTTAGTAGCTCTGCTCCATCACGACAGGGAAATGCACAGAGACCAGAAGTACGTATTGTAACATGGAATAATGATGAACTTTCAACAGATGCCTTACCTGTACATGGCTTTGAGCATTACAAGGCAAATGACTATTCCCTTGCACATGCTCCTTTCTCAGGTAGCAGCTATGCTGGTGGCCAGTGGGCCGAAGGTGATGAACCTCTGTACTATATTGTTTCCCCAAAGGATGTAGTCATTGCAAAACCGAGAGATGCGGAAGATCATATTGCTTGGCTTCTTCAACATGGTTGGCATGAAAAAGCTTTGGCTGCAGTTGAAGCGGGCCAGGGACGGAGTGAACTTCTTGATGAGGTTGGTTCTAGATACCTTGATCATTTGATTGTGGAAAGAAAATATGCTGAAGCCGCATCTTTATGCCCTAAATTGTTGCGAGGATCTGCTTCAGCATGGGAAAGATGGGTTTTCCACTTTGCTCATTTGCGTCAGCTTCCCGTGTTAGTCCCATATATTCCAACAGAAAACCCAAGACTGCGTGATACTGCTTATGAGGTTGCTCTTGTAGCTCTCGCTACAAATCCGTCTTTTCATAAGGAGCTTGTGTCAACTGTTAAATCTTGGCCACCCGTGATCTACTCTTCACTGCCTGTTATCTCAGCTATAGAACCTCAGCTTAATACATCGTCCATGACAGATGCCCTTAAGGAGGCACTAGCAGTGTTGTATGAGATTGATGGGCAATATGAGAAAGCATTCGCACTATATGCTGATCTTTTGAAGCCAGACATATTTTCCTTCATTGAAAAGCACAATCTGTATGATTCGATTCGGGAAAAGGTTGTCCAATTAATGATGCTAGATTGCAAGCTTGCAGTTCCTTTGTTGATCCAAAACAAGGATTTAATAACTCCATCTGAAGTTGTCAACCAACTTCTGAATGCAAGTGATAAGTGTGATTCCAGATATTTCTTGCATGCATACTTGCATTCACTATTTGAAGCAGACCCTCATTCTGGAAAAGAATTTCACGATATGCAGGTAGAGCTTTATGCAGAATATGATCCAAAGATGCTACTTCCTTTTCTTCGCAGTAGTCAACATTATACCCTGGAGAAGGCATATGAAATTTGTGTCGGAAGAGATCTTTTAAAGGAGCAAGTCTTCATTCTTGGAAGAATGGGAAATACTAAACAAGCCCTTGCTGTAATCATAAATAAACTTGGGGATATTGAAGAGGCTGTAGAATTTGTAAATATGCAACATGATGATGAACTCTGGGAAGAGTTAATCCAGCAGTGTCTTCATAAACCTGAAATGGTAGGTGTGTTGTTGGAGCACACAGTTGGAAACCTAGATCCTCTTTATATTGTGAATATGGTTCCCAACGGATTGGAGATTCCTAGGCTTAGGGATCGGCTAGTCAAAATCATCACCAATTACAGGACCGAAACATCTCTAAGAAATGGGTGCAATGATATTCTTAAGGCTGATATCGTCAACCTCTTGGTCAAATACTACAAAGAGGCAACACATGGAATTTACTTGAGCAATGAAGAAGATGAGGCACGGTCAAAGAGAAATGATAGTAGGGCTTCTCAGGTGATTGAGAAATCGGTAGGTGTGAGAAGTATGGAGGTTAAATCAAAACCTAGGGGCGGTGCAAGGTGTTGCATGTGTTTCGATCCTTTTTCTATTCAAAATGTTAATGTCATTGTTTTCTTTTGCTGTCATGCTTATCATGTGACTTGTCTTATGGATTCCACCTATACGAGTGAAATGAAAGCCAGTGTGGCCAATCCTAATGAGAAAGTAGACGATTATGGGTATGGTGATAGTGATGGTGACGATGATGGTGATGATGATGATGACACCCAATCGGGTGGCTCTCGCATGCGTTGTATCTTATGTACTACTGCTGCTAGTGGGGTGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0006605 GO:0006623 GO:0006810 GO:0006886 GO:0006996 GO:0007033 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0019899 GO:0030897 GO:0031338 GO:0031410 GO:0031982 GO:0032879 GO:0032991 GO:0033036 GO:0033043 GO:0033263 GO:0033365 GO:0034613 GO:0035542 GO:0042144 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043254 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048284 GO:0050789 GO:0050794 GO:0051020 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060627 GO:0061024 GO:0061025 GO:0065007 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0097576 GO:0097708 GO:0098796 GO:0099023

KEGG Pathways

Metabolic & Signaling

ko04138 map04138

Pfam Domains

Protein Families

Clathrin

Protein Analysis

963

Amino Acids

107.97

Weight (kDa)

4.86

Isoelectric Point (pI)

44.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_Vps41	PF23411	44 - 400	6.8e-99	Vps42 beta-propeller
TPR_Vps41	PF23556	436 - 776	5.1e-148	Vps41 TPR-like region
Clathrin	PF00637	629 - 754	1.5e-09	Region in Clathrin and VPS
zf-RING_Vps41	PF23555	873 - 912	1.7e-13	Vps41 C-terminal RING finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005429)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08190
fragaria_vesca	FvH4_3g30370 FvH4_3g30370 FvH4_3g30370 FvH4_3g30370 FvH4_3g30370
malus_domestica	MD03G1129800.v1.1 MD11G1151700.v1.1
prunus_persica	Prupe.6G114800_v2.0.a1 Prupe.6G114800_v2.0.a1
pyrus_communis	pycom11g12380 pycom11g12390
rosa_chinensis	RchiOBHm_Chr5g0056311
rosa_laevigata	RLG00000035067 RLG00000035070 RLG00000035077 RLG00000035080 RLG00000035082
rosa_multiflora	Rmu_sc0000680.1_g000003
rosa_roxburghii	Rroxscaffold_1G00024010
rosa_rugosa	Rorug05G0298600 Rorug05G0298700 Rorug05G0298800
rosa_samantha	Rh5AG369600 Rh5BG380500 Rh5CG404000 Rh5DG393600
rosa_wichuraiana	Rw0G004390 Rw0G012630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	605
Acc36I	ACCTGC	2 cut(s)	605, 1981
AccB1I	GGYRCC	1 cut(s)	236
AccB7I	CCANNNNNTGG	2 cut(s)	145, 827
AccI	GTMKAC	1 cut(s)	2766
AccII	CGCG	1 cut(s)	218
AciI	CCGC	6 cut(s)	14, 234, 1211, 1277, 1362, 2610
AclWI	GGATC	8 cut(s)	434, 447, 1399, 1834, 2009, 2303, 2373, 2630
AcoI	YGGCCR	3 cut(s)	1132, 1574, 2745
AcsI	RAATTY	7 cut(s)	278, 332, 953, 1976, 2079, 2198, 2490
AcuI	CTGAAG	3 cut(s)	1377, 1383, 1890
AcyI	GRCGYC	2 cut(s)	27, 185
AfaI	GTAC	6 cut(s)	137, 629, 1005, 1056, 1163, 2863
AflII	CTTAAG	2 cut(s)	1655, 2435
AjiI	CACGTC	1 cut(s)	686
AjnI	CCWGG	3 cut(s)	435, 1281, 2061
AleI	CACNNNNGTG	1 cut(s)	552
Alw21I	GWGCWC	1 cut(s)	2292
Alw26I	GTCTC	2 cut(s)	596, 988
AlwI	GGATC	8 cut(s)	434, 447, 1399, 1834, 2009, 2303, 2373, 2630
Ama87I	CYCGRG	2 cut(s)	221, 260
AoxI	GGCC	7 cut(s)	3, 668, 1132, 1140, 1279, 1574, 2745
ApeKI	GCWGC	5 cut(s)	153, 296, 1119, 1265, 2870
ApoI	RAATTY	7 cut(s)	278, 332, 953, 1976, 2079, 2198, 2490
AseI	ATTAAT	1 cut(s)	1802
Asp700I	GAANNNNTTC	2 cut(s)	346, 1706
AspA2I	CCTAGG	2 cut(s)	2354, 2603
AspLEI	GCGC	1 cut(s)	220
AspS9I	GGNCC	5 cut(s)	4, 560, 1140, 1279, 2397
AsuHPI	GGTGA	9 cut(s)	341, 566, 911, 1160, 2377, 2566, 2795, 2807, 2819
AvaI	CYCGRG	2 cut(s)	221, 260
AvaII	GGWCC	2 cut(s)	560, 2397
AvrII	CCTAGG	2 cut(s)	2354, 2603
BaeI	ACNNNNGTAYC	2 cut(s)	894, 927
BalI	TGGCCA	3 cut(s)	1134, 1576, 2747
BamHI	GGATCC	1 cut(s)	439
BanI	GGYRCC	1 cut(s)	236
BarI	GAAGNNNNNNTAC	2 cut(s)	549, 581
BauI	CACGAG	1 cut(s)	672
BbsI	GAAGAC	2 cut(s)	2107, 2246
Bbv12I	GWGCWC	1 cut(s)	2292
BbvCI	CCTCAGC	1 cut(s)	1623
BbvI	GCAGC	5 cut(s)	165, 283, 1131, 1252, 2857
BccI	CCATC	9 cut(s)	350, 513, 935, 979, 1407, 1682, 1873, 2786, 2798
BceAI	ACGGC	3 cut(s)	40, 182, 247
BcgI	CGANNNNNNTGC	4 cut(s)	181, 215, 2614, 2648
BciT130I	CCWGG	3 cut(s)	437, 1283, 2063
BclI	TGATCA	2 cut(s)	643, 1327
BcoDI	GTCTC	2 cut(s)	596, 988
BfmI	CTRYAG	4 cut(s)	297, 1266, 1614, 2193
BfrI	CTTAAG	2 cut(s)	1655, 2435
BfuAI	ACCTGC	2 cut(s)	605, 1981
BglI	GCCNNNNNGGC	1 cut(s)	2744
BglII	AGATCT	1 cut(s)	2095
BisI	GCNGC	7 cut(s)	154, 235, 297, 1120, 1266, 1362, 2871
BlnI	CCTAGG	2 cut(s)	2354, 2603
BlsI	GCNGC	7 cut(s)	155, 236, 298, 1121, 1267, 1363, 2872
Bme1390I	CCNGG	3 cut(s)	437, 1283, 2063
Bme18I	GGWCC	2 cut(s)	560, 2397
BmeT110I	CYCGRG	2 cut(s)	221, 260
BmgBI	CACGTC	1 cut(s)	686
BmgT120I	GGNCC	5 cut(s)	4, 560, 1140, 1279, 2397
BmiI	GGNNCC	5 cut(s)	6, 123, 238, 441, 2334
BmrFI	CCNGG	3 cut(s)	437, 1283, 2063
BmsI	GCATC	8 cut(s)	601, 863, 1033, 1198, 1373, 1639, 1797, 2013
BpiI	GAAGAC	2 cut(s)	2107, 2246
BpmI	CTGGAG	1 cut(s)	2084
Bpu10I	CCTNAGC	3 cut(s)	690, 1623, 2360
BpuEI	CTTGAG	2 cut(s)	116, 2518
Bsa29I	ATCGAT	1 cut(s)	770
BsaAI	YACGTR	1 cut(s)	1007
BsaHI	GRCGYC	2 cut(s)	27, 185
BsaI	GGTCTC	1 cut(s)	988
BsaJI	CCNNGG	6 cut(s)	125, 435, 1282, 2061, 2354, 2603
BsaXI	ACNNNNNCTCC	4 cut(s)	574, 604, 1541, 1571
Bse1I	ACTGG	2 cut(s)	1135, 2739
Bse3DI	GCAATG	4 cut(s)	594, 1191, 2431, 2509
BseBI	CCWGG	3 cut(s)	437, 1283, 2063
BseCI	ATCGAT	1 cut(s)	770
BseDI	CCNNGG	6 cut(s)	125, 435, 1282, 2061, 2354, 2603
BseGI	GGATG	3 cut(s)	727, 854, 1189
BseMI	GCAATG	4 cut(s)	594, 1191, 2431, 2509
BseMII	CTCAG	5 cut(s)	681, 1125, 1623, 1637, 2564
BseNI	ACTGG	2 cut(s)	1135, 2739
BseRI	GAGGAG	3 cut(s)	77, 98, 134
BseXI	GCAGC	5 cut(s)	165, 283, 1131, 1252, 2857
BseYI	CCCAGC	2 cut(s)	523, 744
Bsh1236I	CGCG	1 cut(s)	218
BshFI	GGCC	7 cut(s)	5, 670, 1134, 1142, 1281, 1576, 2747
BshNI	GGYRCC	1 cut(s)	236
BshVI	ATCGAT	1 cut(s)	770
BsiHKAI	GWGCWC	1 cut(s)	2292
BsiHKCI	CYCGRG	2 cut(s)	221, 260
BslFI	GGGAC	5 cut(s)	238, 803, 817, 1299, 1442
BsmAI	GTCTC	2 cut(s)	596, 988
BsmFI	GGGAC	5 cut(s)	238, 803, 817, 1299, 1442
BsmI	GAATGC	4 cut(s)	156, 1706, 1897, 1939
BsnI	GGCC	7 cut(s)	5, 670, 1134, 1142, 1281, 1576, 2747
Bso31I	GGTCTC	1 cut(s)	988
BsoBI	CYCGRG	2 cut(s)	221, 260
Bsp1286I	GDGCHC	1 cut(s)	2292
Bsp1407I	TGTACA	2 cut(s)	627, 1054
BspACI	CCGC	6 cut(s)	14, 234, 1211, 1277, 1362, 2610
BspANI	GGCC	7 cut(s)	5, 670, 1134, 1142, 1281, 1576, 2747
BspCNI	CTCAG	5 cut(s)	682, 1124, 1622, 1636, 2563
BspDI	ATCGAT	1 cut(s)	770
BspFNI	CGCG	1 cut(s)	218
BspLI	GGNNCC	5 cut(s)	6, 123, 238, 441, 2334
BspMAI	CTGCAG	2 cut(s)	301, 1270
BspMI	ACCTGC	2 cut(s)	605, 1981
BspPI	GGATC	8 cut(s)	434, 447, 1399, 1834, 2009, 2303, 2373, 2630
BspT107I	GGYRCC	1 cut(s)	236
BspTI	CTTAAG	2 cut(s)	1655, 2435
BspTNI	GGTCTC	1 cut(s)	988
BsrDI	GCAATG	4 cut(s)	594, 1191, 2431, 2509
BsrGI	TGTACA	2 cut(s)	627, 1054
BsrI	ACTGG	2 cut(s)	1135, 2739
BssECI	CCNNGG	6 cut(s)	125, 435, 1282, 2061, 2354, 2603
BssNI	GRCGYC	2 cut(s)	27, 185
BssSI	CACGAG	1 cut(s)	672
BssT1I	CCWWGG	2 cut(s)	2354, 2603
Bst2BI	CACGAG	1 cut(s)	672
Bst2UI	CCWGG	3 cut(s)	437, 1283, 2063
Bst4CI	ACNGT	5 cut(s)	168, 241, 1564, 2296, 2523
Bst6I	CTCTTC	9 cut(s)	48, 69, 75, 105, 111, 1597, 2086, 2181, 2229
BstACI	GRCGYC	2 cut(s)	27, 185
BstAFI	CTTAAG	2 cut(s)	1655, 2435
BstAPI	GCANNNNNTGC	1 cut(s)	1718
BstAUI	TGTACA	2 cut(s)	627, 1054
BstBAI	YACGTR	1 cut(s)	1007
BstC8I	GCNNGC	5 cut(s)	1279, 1820, 1824, 1929, 2846
BstDEI	CTNAG	8 cut(s)	311, 690, 1111, 1609, 1623, 2360, 2411, 2550
BstENI	CCTNNNNNAGG	3 cut(s)	1110, 1658, 2066
BstF5I	GGATG	3 cut(s)	727, 854, 1189
BstFNI	CGCG	1 cut(s)	218
BstHHI	GCGC	1 cut(s)	220
BstMAI	GTCTC	2 cut(s)	596, 988
BstNI	CCWGG	3 cut(s)	437, 1283, 2063
BstNSI	RCATGY	5 cut(s)	572, 1103, 1931, 2629, 2848
BstSCI	CCNGG	3 cut(s)	435, 1281, 2061
BstSFI	CTRYAG	4 cut(s)	297, 1266, 1614, 2193
BstSNI	TACGTA	1 cut(s)	1007
BstUI	CGCG	1 cut(s)	218
BstV1I	GCAGC	5 cut(s)	165, 283, 1131, 1252, 2857
BstV2I	GAAGAC	2 cut(s)	2107, 2246
BstX2I	RGATCY	4 cut(s)	439, 1391, 2095, 2308
BstXI	CCANNNNNNTGG	2 cut(s)	2344, 2834
BstYI	RGATCY	4 cut(s)	439, 1391, 2095, 2308
Bsu15I	ATCGAT	1 cut(s)	770
BsuRI	GGCC	7 cut(s)	5, 670, 1134, 1142, 1281, 1576, 2747
BsuTUI	ATCGAT	1 cut(s)	770
BtgZI	GCGATG	2 cut(s)	192, 398
BtrI	CACGTC	1 cut(s)	686
BtsCI	GGATG	3 cut(s)	727, 854, 1189
BtsI	GCAGTG	5 cut(s)	306, 579, 1595, 1677, 2255
BtsIMutI	CAGTG	9 cut(s)	164, 306, 384, 579, 1142, 1595, 1677, 2255, 2746
BveI	ACCTGC	2 cut(s)	605, 1981
Cac8I	GCNNGC	5 cut(s)	1279, 1820, 1824, 1929, 2846
CfoI	GCGC	1 cut(s)	220
Cfr13I	GGNCC	5 cut(s)	4, 560, 1140, 1279, 2397
ClaI	ATCGAT	1 cut(s)	770
CseI	GACGC	3 cut(s)	16, 174, 1427
Csp6I	GTAC	6 cut(s)	136, 628, 1004, 1055, 1162, 2862
CviQI	GTAC	6 cut(s)	136, 628, 1004, 1055, 1162, 2862
DdeI	CTNAG	8 cut(s)	311, 690, 1111, 1609, 1623, 2360, 2411, 2550
DraI	TTTAAA	2 cut(s)	532, 2103
EaeI	YGGCCR	3 cut(s)	1132, 1574, 2745
Eam1104I	CTCTTC	9 cut(s)	48, 69, 75, 105, 111, 1597, 2086, 2181, 2229
EarI	CTCTTC	9 cut(s)	48, 69, 75, 105, 111, 1597, 2086, 2181, 2229
Eco105I	TACGTA	1 cut(s)	1007
Eco130I	CCWWGG	2 cut(s)	2354, 2603
Eco31I	GGTCTC	1 cut(s)	988
Eco32I	GATATC	1 cut(s)	2446
Eco47I	GGWCC	2 cut(s)	560, 2397
Eco57I	CTGAAG	3 cut(s)	1377, 1383, 1890
Eco88I	CYCGRG	2 cut(s)	221, 260
EcoNI	CCTNNNNNAGG	3 cut(s)	1110, 1658, 2066
EcoO109I	RGGNCCY	1 cut(s)	560
EcoRI	GAATTC	1 cut(s)	278
EcoRII	CCWGG	3 cut(s)	435, 1281, 2061
EcoRV	GATATC	1 cut(s)	2446
EcoT14I	CCWWGG	2 cut(s)	2354, 2603
EcoT22I	ATGCAT	1 cut(s)	1933
ErhI	CCWWGG	2 cut(s)	2354, 2603
FaqI	GGGAC	5 cut(s)	238, 803, 817, 1299, 1442
FauI	CCCGC	1 cut(s)	1270
FauNDI	CATATG	1 cut(s)	2074
FbaI	TGATCA	2 cut(s)	643, 1327
FblI	GTMKAC	1 cut(s)	2766
Fnu4HI	GCNGC	7 cut(s)	154, 235, 297, 1120, 1266, 1362, 2871
FokI	GGATG	3 cut(s)	734, 841, 1196
Fsp4HI	GCNGC	7 cut(s)	154, 235, 297, 1120, 1266, 1362, 2871
GlaI	GCGC	1 cut(s)	219
GluI	GCNGC	7 cut(s)	154, 235, 297, 1120, 1266, 1362, 2871
GsaI	CCCAGC	2 cut(s)	527, 748
GsuI	CTGGAG	1 cut(s)	2084
HaeIII	GGCC	7 cut(s)	5, 670, 1134, 1142, 1281, 1576, 2747
HgaI	GACGC	3 cut(s)	16, 174, 1427
HhaI	GCGC	1 cut(s)	220
Hin1I	GRCGYC	2 cut(s)	27, 185
Hin6I	GCGC	1 cut(s)	218
HinP1I	GCGC	1 cut(s)	218
HincII	GTYRAC	5 cut(s)	171, 1560, 1879, 2051, 2452
HindII	GTYRAC	5 cut(s)	171, 1560, 1879, 2051, 2452
HindIII	AAGCTT	2 cut(s)	1257, 1820
HpaI	GTTAAC	1 cut(s)	171
HphI	GGTGA	9 cut(s)	341, 566, 911, 1160, 2377, 2566, 2795, 2807, 2819
Hpy166II	GTNNAC	8 cut(s)	171, 628, 1295, 1560, 1879, 2051, 2452, 2767
Hpy188I	TCNGA	5 cut(s)	352, 864, 1870, 1890, 2090
Hpy8I	GTNNAC	8 cut(s)	171, 628, 1295, 1560, 1879, 2051, 2452, 2767
Hpy99I	CGWCG	5 cut(s)	32, 41, 44, 190, 203
HpyAV	CCTTC	8 cut(s)	26, 320, 418, 551, 943, 1139, 1759, 2062
HpyCH4III	ACNGT	5 cut(s)	168, 241, 1564, 2296, 2523
HpyCH4IV	ACGT	3 cut(s)	649, 685, 1006
HpyF3I	CTNAG	8 cut(s)	311, 690, 1111, 1609, 1623, 2360, 2411, 2550
HpySE526I	ACGT	3 cut(s)	649, 685, 1006
Hsp92I	GRCGYC	2 cut(s)	27, 185
HspAI	GCGC	1 cut(s)	218
Ksp22I	TGATCA	2 cut(s)	643, 1327
KspAI	GTTAAC	1 cut(s)	171
LmnI	GCTCC	6 cut(s)	121, 973, 1108, 1549, 2107, 2287
Lsp1109I	GCAGC	5 cut(s)	165, 283, 1131, 1252, 2857
LweI	GCATC	8 cut(s)	601, 863, 1033, 1198, 1373, 1639, 1797, 2013
MaeII	ACGT	3 cut(s)	649, 685, 1006
MaeIII	GTNAC	3 cut(s)	1012, 2695, 2795
MflI	RGATCY	4 cut(s)	439, 1391, 2095, 2308
MhlI	GDGCHC	1 cut(s)	2292
MlsI	TGGCCA	3 cut(s)	1134, 1576, 2747
MluNI	TGGCCA	3 cut(s)	1134, 1576, 2747
MlyI	GAGTC	3 cut(s)	220, 598, 719
MmeI	TCCRAC	4 cut(s)	1493, 2068, 2265, 2278
Mox20I	TGGCCA	3 cut(s)	1134, 1576, 2747
Mph1103I	ATGCAT	1 cut(s)	1933
MroXI	GAANNNNTTC	2 cut(s)	346, 1706
MscI	TGGCCA	3 cut(s)	1134, 1576, 2747
MslI	CAYNNNNRTG	3 cut(s)	552, 1369, 2220
Msp20I	TGGCCA	3 cut(s)	1134, 1576, 2747
MspCI	CTTAAG	2 cut(s)	1655, 2435
MspR9I	CCNGG	3 cut(s)	437, 1283, 2063
Mva1269I	GAATGC	4 cut(s)	156, 1706, 1897, 1939
MvaI	CCWGG	3 cut(s)	437, 1283, 2063
MvnI	CGCG	1 cut(s)	218
NdeI	CATATG	1 cut(s)	2074
NlaIV	GGNNCC	5 cut(s)	6, 123, 238, 441, 2334
NmeAIII	GCCGAG	1 cut(s)	150
NmuCI	GTSAC	2 cut(s)	2695, 2795
NsiI	ATGCAT	1 cut(s)	1933
NspI	RCATGY	5 cut(s)	572, 1103, 1931, 2629, 2848
OliI	CACNNNNGTG	1 cut(s)	552
PaeI	GCATGC	2 cut(s)	1931, 2848
PaqCI	CACCTGC	1 cut(s)	605
PcsI	WCGNNNNNNNCGW	2 cut(s)	36, 237
PctI	GAATGC	4 cut(s)	156, 1706, 1897, 1939
PdmI	GAANNNNTTC	2 cut(s)	346, 1706
PfeI	GAWTC	7 cut(s)	10, 265, 1775, 1780, 1910, 2350, 2711
PflMI	CCANNNNNTGG	2 cut(s)	145, 827
PkrI	GCNGC	7 cut(s)	155, 236, 298, 1121, 1267, 1363, 2872
PleI	GAGTC	3 cut(s)	220, 597, 719
PpsI	GAGTC	3 cut(s)	220, 597, 719
Ppu21I	YACGTR	1 cut(s)	1007
PpuMI	RGGWCCY	1 cut(s)	560
PshBI	ATTAAT	1 cut(s)	1802
Psp5II	RGGWCCY	1 cut(s)	560
Psp6I	CCWGG	3 cut(s)	435, 1281, 2061
PspFI	CCCAGC	2 cut(s)	523, 744
PspGI	CCWGG	3 cut(s)	435, 1281, 2061
PspN4I	GGNNCC	5 cut(s)	6, 123, 238, 441, 2334
PspPI	GGNCC	5 cut(s)	4, 560, 1140, 1279, 2397
PspPPI	RGGWCCY	1 cut(s)	560
PstI	CTGCAG	2 cut(s)	301, 1270
PsuI	RGATCY	4 cut(s)	439, 1391, 2095, 2308
RsaI	GTAC	6 cut(s)	137, 629, 1005, 1056, 1163, 2863
RsaNI	GTAC	6 cut(s)	136, 628, 1004, 1055, 1162, 2862
RseI	CAYNNNNRTG	3 cut(s)	552, 1369, 2220
SatI	GCNGC	7 cut(s)	154, 235, 297, 1120, 1266, 1362, 2871
Sau96I	GGNCC	5 cut(s)	4, 560, 1140, 1279, 2397
SchI	GAGTC	3 cut(s)	220, 598, 719
ScrFI	CCNGG	3 cut(s)	437, 1283, 2063
SduI	GDGCHC	1 cut(s)	2292
SfaNI	GCATC	8 cut(s)	601, 863, 1033, 1198, 1373, 1639, 1797, 2013
SfcI	CTRYAG	4 cut(s)	297, 1266, 1614, 2193
SinI	GGWCC	2 cut(s)	560, 2397
SmiMI	CAYNNNNRTG	3 cut(s)	552, 1369, 2220
SmlI	CTYRAG	4 cut(s)	131, 1655, 2435, 2497
SmoI	CTYRAG	4 cut(s)	131, 1655, 2435, 2497
SnaBI	TACGTA	1 cut(s)	1007
SphI	GCATGC	2 cut(s)	1931, 2848
SsiI	CCGC	6 cut(s)	14, 234, 1211, 1277, 1362, 2610
StyD4I	CCNGG	3 cut(s)	435, 1281, 2061
StyI	CCWWGG	2 cut(s)	2354, 2603
TaaI	ACNGT	5 cut(s)	168, 241, 1564, 2296, 2523
TaiI	ACGT	3 cut(s)	652, 688, 1009
TaqI	TCGA	6 cut(s)	30, 252, 318, 770, 1778, 2634
TaqII	GACCGA	1 cut(s)	2414
TatI	WGTACW	4 cut(s)	627, 1054, 1161, 2861
TauI	GCSGC	2 cut(s)	237, 1364
TfiI	GAWTC	7 cut(s)	10, 265, 1775, 1780, 1910, 2350, 2711
TscAI	CASTG	9 cut(s)	171, 306, 391, 579, 1142, 1602, 1677, 2255, 2746
TseFI	GTSAC	2 cut(s)	2695, 2795
TseI	GCWGC	5 cut(s)	153, 296, 1119, 1265, 2870
Tsp45I	GTSAC	2 cut(s)	2695, 2795
TspGWI	ACGGA	3 cut(s)	1303, 1524, 2355
TspRI	CASTG	9 cut(s)	171, 306, 391, 579, 1142, 1602, 1677, 2255, 2746
Van91I	CCANNNNNTGG	2 cut(s)	145, 827
Vha464I	CTTAAG	2 cut(s)	1655, 2435
VpaK11BI	GGWCC	2 cut(s)	560, 2397
VspI	ATTAAT	1 cut(s)	1802
XagI	CCTNNNNNAGG	3 cut(s)	1110, 1658, 2066
XapI	RAATTY	7 cut(s)	278, 332, 953, 1976, 2079, 2198, 2490
XbaI	TCTAGA	3 cut(s)	464, 788, 1316
XceI	RCATGY	5 cut(s)	572, 1103, 1931, 2629, 2848
XmaJI	CCTAGG	2 cut(s)	2354, 2603
XmiI	GTMKAC	1 cut(s)	2766
XmnI	GAANNNNTTC	2 cut(s)	346, 1706
Zsp2I	ATGCAT	1 cut(s)	1933

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.