MD11G1260100.v1.1

protein At3g15000, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009062
Physical Location & Seq
Reverse (-)
37419888 .. 37423084
3197 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1260100.v1.1.491

Sequence Viewer

Length: 1170 bp
ATGGCGACCCATTTCTTCTACCGCTCGCTTCCCAAGACCCAAGCCATGGCTTCATTTCTCTCTCGTTCCCTCACCACCGCCACCTCCGCCGCTTCCCTATCCACCGCCGCCCGATCTCGCTCTTCTTCGCTCTCTCTCCTCCACCGCCTCCGCCCCCTCGCCGGAGTCATGGCCTCCGCCGGCAGGCTCTCTCCTGCCATCGTCCGGTGCTTGTCCACCAGATCGACCACGTCGTCGCTCAGAGACCCAAACCCTAACTGGTCGAACCGGCCTCCCAAGGAGACGATCCTCCTCGATGGGTGTGACTTCGAGCACTGGTTGGTTGTCATGGAGCCCCCTCAGGGTGACATCACTAGGGATGAGATCATCAATAGCTACATCAAAACCCTAGCTACCGTCGTCGGAAGTGAGGAAGAAGCTAGAATGAAGATCTACTCAGTTTCAACTAGGTGCTATTATGCATTTGGGGCACTTGTATCTGAAGAGGTTTCACTCAAAATCAAAGAGTTGCCTGGAGTCCGTTGGGTACTTCCTGATTCTTACTTGGATGTTAAGAACAAAGATTACGGAGGTGAACCTTTTATTAATGGCCAAGCTGTGCCATATGATCCCAAGTACCATGAAGAATGGATAAGAAACAATAGTCGAGCAAATGAGAGAAATAGACGTAATGACAGACCTCGTAACTTTGACAGATCCAGGAACTTTGAAAGGAGGAGGGAAAATACCGGTCCATCTCCTATGCCTAATCAAGCTGGCCCAAATTCGGGACCTGGACCTGCCAACATGGGTCCTCCACCCCCCAACAGGGGTCCTCCACCTCCCAACATGGGTCCAGGCCCGATGCCTCCCAACATGGGTCCTCCACCCCCCAACAGGGCTCCAATGCCGCCTCACAACATGGGTCCACCAAACAACTACAATAATCCCCCGCCAAGCAGCAACTGGAACTCTGGGCCTCCCAACAACTACAATCAGGCACCTCCCAACAACTACAATCAGGTGCCTCCCAACAACTACAATCAGATGCCTCCCAATAACCTGGGAGGGGTGCCACCAAACAACATGGGTCCGCCATCTAATGCAGGATGGAATGGTGCTGGACAATACCAGAATAACTACACGCCAGAGAGGGACGGTGGAGCCAATCCTGGCCCAAACCGTTACTAA

Protein Analysis

390

Amino Acids

42.84

Weight (kDa)

9.63

Isoelectric Point (pI)

60.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MORF_dom PF21864 103 - 194 1.1e-38 Multiple organellar RNA editing factor 1, MORF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 232
Acc36I ACCTGC 1 cut(s) 787
AccB1I GGYRCC 3 cut(s) 979, 1003, 1051
AccB7I CCANNNNNTGG 1 cut(s) 46
AccBSI CCGCTC 1 cut(s) 24
AclWI GGATC 3 cut(s) 280, 602, 690
AcoI YGGCCR 1 cut(s) 589
AcsI RAATTY 1 cut(s) 763
AcuI CTGAAG 1 cut(s) 501
AfaI GTAC 2 cut(s) 528, 617
AgeI ACCGGT 1 cut(s) 728
AgsI TTSAA 2 cut(s) 444, 710
AjiI CACGTC 1 cut(s) 231
AjnI CCWGG 6 cut(s) 511, 698, 772, 835, 1041, 1150
AluBI AGCT 5 cut(s) 375, 392, 419, 596, 755
AluI AGCT 5 cut(s) 375, 392, 419, 596, 755
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 237, 275
AlwI GGATC 3 cut(s) 280, 602, 690
AlwNI CAGNNNCTG 1 cut(s) 945
AoxI GGCC 7 cut(s) 171, 269, 589, 757, 838, 956, 1153
ApeKI GCWGC 1 cut(s) 939
ApoI RAATTY 1 cut(s) 763
AseI ATTAAT 1 cut(s) 585
AsiGI ACCGGT 1 cut(s) 728
AsuHPI GGTGA 3 cut(s) 64, 356, 584
AvaII GGWCC 9 cut(s) 731, 770, 776, 791, 812, 833, 860, 905, 1070
AxyI CCTNAGG 1 cut(s) 339
BaeGI GKGCMC 1 cut(s) 472
BalI TGGCCA 1 cut(s) 591
BanI GGYRCC 3 cut(s) 979, 1003, 1051
BanII GRGCYC 2 cut(s) 336, 883
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 1 cut(s) 951
BccI CCATC 5 cut(s) 206, 290, 742, 1083, 1084
BciT130I CCWGG 6 cut(s) 513, 700, 774, 837, 1043, 1152
BcoDI GTCTC 2 cut(s) 237, 275
BfaI CTAG 4 cut(s) 354, 389, 420, 447
BfuAI ACCTGC 1 cut(s) 787
BglII AGATCT 1 cut(s) 429
BisI GCNGC 4 cut(s) 90, 108, 890, 940
BlsI GCNGC 4 cut(s) 91, 109, 891, 941
Bme1390I CCNGG 6 cut(s) 513, 700, 774, 837, 1043, 1152
Bme18I GGWCC 9 cut(s) 731, 770, 776, 791, 812, 833, 860, 905, 1070
BmgBI CACGTC 1 cut(s) 231
BmrFI CCNGG 6 cut(s) 513, 700, 774, 837, 1043, 1152
BmsI GCATC 2 cut(s) 834, 1017
BpmI CTGGAG 1 cut(s) 534
BsaI GGTCTC 1 cut(s) 237
BsaJI CCNNGG 3 cut(s) 45, 276, 1042
BsaWI WCCGGW 2 cut(s) 204, 728
Bse118I RCCGGY 3 cut(s) 179, 267, 728
Bse1I ACTGG 3 cut(s) 263, 320, 950
Bse21I CCTNAGG 1 cut(s) 339
BseBI CCWGG 6 cut(s) 513, 700, 774, 837, 1043, 1152
BseDI CCNNGG 3 cut(s) 45, 276, 1042
BseGI GGATG 3 cut(s) 364, 553, 1094
BseMII CTCAG 3 cut(s) 253, 353, 450
BseNI ACTGG 3 cut(s) 263, 320, 950
BseRI GAGGAG 3 cut(s) 128, 281, 730
BseSI GKGCMC 1 cut(s) 472
BseXI GCAGC 1 cut(s) 951
BshFI GGCC 7 cut(s) 173, 271, 591, 759, 840, 958, 1155
BshNI GGYRCC 3 cut(s) 979, 1003, 1051
BshTI ACCGGT 1 cut(s) 728
BsiHKAI GWGCWC 1 cut(s) 315
BsiSI CCGG 5 cut(s) 162, 180, 205, 268, 729
BslFI GGGAC 2 cut(s) 783, 1148
BsmAI GTCTC 2 cut(s) 237, 275
BsmBI CGTCTC 1 cut(s) 275
BsmFI GGGAC 2 cut(s) 783, 1148
BsnI GGCC 7 cut(s) 173, 271, 591, 759, 840, 958, 1155
Bso31I GGTCTC 1 cut(s) 237
Bsp1286I GDGCHC 4 cut(s) 315, 336, 472, 883
Bsp143I GATC 7 cut(s) 113, 221, 285, 363, 429, 607, 695
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 7 cut(s) 173, 271, 591, 759, 840, 958, 1155
BspCNI CTCAG 3 cut(s) 252, 352, 449
BspMI ACCTGC 1 cut(s) 787
BspPI GGATC 3 cut(s) 280, 602, 690
BspQI GCTCTTC 1 cut(s) 127
BspT107I GGYRCC 3 cut(s) 979, 1003, 1051
BspTNI GGTCTC 1 cut(s) 237
BsrBI CCGCTC 1 cut(s) 24
BsrFI RCCGGY 3 cut(s) 179, 267, 728
BsrI ACTGG 3 cut(s) 263, 320, 950
BssAI RCCGGY 3 cut(s) 179, 267, 728
BssECI CCNNGG 3 cut(s) 45, 276, 1042
BssMI GATC 7 cut(s) 113, 221, 285, 363, 429, 607, 695
BssT1I CCWWGG 2 cut(s) 45, 276
Bst2UI CCWGG 6 cut(s) 513, 700, 774, 837, 1043, 1152
Bst4CI ACNGT 3 cut(s) 397, 1139, 1163
Bst6I CTCTTC 2 cut(s) 127, 477
BstC8I GCNNGC 4 cut(s) 26, 181, 185, 757
BstDEI CTNAG 3 cut(s) 239, 339, 436
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 3 cut(s) 364, 553, 1094
BstKTI GATC 7 cut(s) 116, 224, 288, 366, 432, 610, 698
BstMAI GTCTC 2 cut(s) 237, 275
BstMBI GATC 7 cut(s) 113, 221, 285, 363, 429, 607, 695
BstMWI GCNNNNNNNGC 2 cut(s) 86, 467
BstNI CCWGG 6 cut(s) 513, 700, 774, 837, 1043, 1152
BstSCI CCNGG 6 cut(s) 511, 698, 772, 835, 1041, 1150
BstSLI GKGCMC 1 cut(s) 472
BstV1I GCAGC 1 cut(s) 951
BstX2I RGATCY 2 cut(s) 429, 695
BstXI CCANNNNNNTGG 1 cut(s) 1042
BstYI RGATCY 2 cut(s) 429, 695
Bsu36I CCTNAGG 1 cut(s) 339
BsuRI GGCC 7 cut(s) 173, 271, 591, 759, 840, 958, 1155
BtgI CCRYGG 1 cut(s) 45
BtrI CACGTC 1 cut(s) 231
BtsCI GGATG 3 cut(s) 364, 553, 1094
BtsIMutI CAGTG 1 cut(s) 313
BveI ACCTGC 1 cut(s) 787
Cac8I GCNNGC 4 cut(s) 26, 181, 185, 757
CaiI CAGNNNCTG 1 cut(s) 945
Cfr10I RCCGGY 3 cut(s) 179, 267, 728
Csp6I GTAC 2 cut(s) 527, 616
CspAI ACCGGT 1 cut(s) 728
CviAII CATG 9 cut(s) 46, 169, 328, 620, 787, 829, 856, 901, 1066
CviQI GTAC 2 cut(s) 527, 616
DdeI CTNAG 3 cut(s) 239, 339, 436
DpnI GATC 7 cut(s) 115, 223, 287, 365, 431, 609, 697
DpnII GATC 7 cut(s) 113, 221, 285, 363, 429, 607, 695
DrdI GACNNNNNNGTC 1 cut(s) 232
DseDI GACNNNNNNGTC 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 589
Eam1104I CTCTTC 2 cut(s) 127, 477
EarI CTCTTC 2 cut(s) 127, 477
EciI GGCGGA 4 cut(s) 76, 140, 166, 1062
Eco130I CCWWGG 2 cut(s) 45, 276
Eco24I GRGCYC 2 cut(s) 336, 883
Eco31I GGTCTC 1 cut(s) 237
Eco47I GGWCC 9 cut(s) 731, 770, 776, 791, 812, 833, 860, 905, 1070
Eco57I CTGAAG 1 cut(s) 501
Eco81I CCTNAGG 1 cut(s) 339
EcoO109I RGGNCCY 4 cut(s) 770, 791, 812, 860
EcoRII CCWGG 6 cut(s) 511, 698, 772, 835, 1041, 1150
EcoT14I CCWWGG 2 cut(s) 45, 276
EcoT22I ATGCAT 1 cut(s) 463
EcoT38I GRGCYC 2 cut(s) 336, 883
ErhI CCWWGG 2 cut(s) 45, 276
Esp3I CGTCTC 1 cut(s) 275
FaeI CATG 9 cut(s) 49, 172, 331, 623, 790, 832, 859, 904, 1069
FaqI GGGAC 2 cut(s) 783, 1148
FatI CATG 9 cut(s) 45, 168, 327, 619, 786, 828, 855, 900, 1065
FauI CCCGC 1 cut(s) 939
FauNDI CATATG 1 cut(s) 604
Fnu4HI GCNGC 4 cut(s) 90, 108, 890, 940
FokI GGATG 3 cut(s) 371, 560, 1101
FriOI GRGCYC 2 cut(s) 336, 883
Fsp4HI GCNGC 4 cut(s) 90, 108, 890, 940
FspBI CTAG 4 cut(s) 354, 389, 420, 447
GluI GCNGC 4 cut(s) 90, 108, 890, 940
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 7 cut(s) 173, 271, 591, 759, 840, 958, 1155
HapII CCGG 5 cut(s) 162, 180, 205, 268, 729
Hin1II CATG 9 cut(s) 49, 172, 331, 623, 790, 832, 859, 904, 1069
HinfI GANTC 3 cut(s) 165, 516, 536
HpaII CCGG 5 cut(s) 162, 180, 205, 268, 729
HphI GGTGA 3 cut(s) 64, 356, 584
Hpy166II GTNNAC 3 cut(s) 216, 575, 908
Hpy188I TCNGA 4 cut(s) 242, 404, 481, 1026
Hpy188III TCNNGA 2 cut(s) 533, 768
Hpy8I GTNNAC 3 cut(s) 216, 575, 908
Hpy99I CGWCG 4 cut(s) 235, 238, 401, 404
HpyCH4III ACNGT 3 cut(s) 397, 1139, 1163
HpyCH4IV ACGT 2 cut(s) 230, 667
HpyCH4V TGCA 2 cut(s) 461, 1085
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 467
HpyF3I CTNAG 3 cut(s) 239, 339, 436
HpySE526I ACGT 2 cut(s) 230, 667
Hsp92II CATG 9 cut(s) 49, 172, 331, 623, 790, 832, 859, 904, 1069
KroI GCCGGC 1 cut(s) 179
KroNI GCCGGC 1 cut(s) 181
Kzo9I GATC 7 cut(s) 113, 221, 285, 363, 429, 607, 695
LguI GCTCTTC 1 cut(s) 127
LmnI GCTCC 3 cut(s) 331, 886, 1142
Lsp1109I GCAGC 1 cut(s) 951
LweI GCATC 2 cut(s) 834, 1017
MaeI CTAG 4 cut(s) 354, 389, 420, 447
MaeII ACGT 2 cut(s) 230, 667
MaeIII GTNAC 4 cut(s) 302, 344, 683, 1163
MalI GATC 7 cut(s) 115, 223, 287, 365, 431, 609, 697
MbiI CCGCTC 1 cut(s) 24
MboI GATC 7 cut(s) 113, 221, 285, 363, 429, 607, 695
MboII GAAGA 7 cut(s) 7, 114, 117, 425, 439, 494, 635
MflI RGATCY 2 cut(s) 429, 695
MhlI GDGCHC 4 cut(s) 315, 336, 472, 883
MlsI TGGCCA 1 cut(s) 591
MluCI AATT 1 cut(s) 763
MluNI TGGCCA 1 cut(s) 591
MlyI GAGTC 2 cut(s) 174, 525
MmeI TCCRAC 1 cut(s) 382
Mox20I TGGCCA 1 cut(s) 591
Mph1103I ATGCAT 1 cut(s) 463
MroNI GCCGGC 1 cut(s) 179
MscI TGGCCA 1 cut(s) 591
MseI TTAA 2 cut(s) 552, 585
Msp20I TGGCCA 1 cut(s) 591
MspI CCGG 5 cut(s) 162, 180, 205, 268, 729
MspR9I CCNGG 6 cut(s) 513, 700, 774, 837, 1043, 1152
MvaI CCWGG 6 cut(s) 513, 700, 774, 837, 1043, 1152
MwoI GCNNNNNNNGC 2 cut(s) 86, 467
NaeI GCCGGC 1 cut(s) 181
NcoI CCATGG 1 cut(s) 45
NdeI CATATG 1 cut(s) 604
NdeII GATC 7 cut(s) 113, 221, 285, 363, 429, 607, 695
NgoMIV GCCGGC 1 cut(s) 179
NlaIII CATG 9 cut(s) 49, 172, 331, 623, 790, 832, 859, 904, 1069
NmuCI GTSAC 2 cut(s) 302, 344
NsiI ATGCAT 1 cut(s) 463
PciSI GCTCTTC 1 cut(s) 127
PcsI WCGNNNNNNNCGW 1 cut(s) 230
PdiI GCCGGC 1 cut(s) 181
PfeI GAWTC 1 cut(s) 536
PflFI GACNNNGTC 1 cut(s) 229
PflMI CCANNNNNTGG 1 cut(s) 46
PfoI TCCNGGA 1 cut(s) 698
PinAI ACCGGT 1 cut(s) 728
PkrI GCNGC 4 cut(s) 91, 109, 891, 941
PleI GAGTC 2 cut(s) 173, 524
PpsI GAGTC 2 cut(s) 173, 524
PpuMI RGGWCCY 4 cut(s) 770, 791, 812, 860
PshBI ATTAAT 1 cut(s) 585
Psp5II RGGWCCY 4 cut(s) 770, 791, 812, 860
Psp6I CCWGG 6 cut(s) 511, 698, 772, 835, 1041, 1150
PspGI CCWGG 6 cut(s) 511, 698, 772, 835, 1041, 1150
PspPPI RGGWCCY 4 cut(s) 770, 791, 812, 860
PsrI GAACNNNNNNTAC 2 cut(s) 548, 580
PstNI CAGNNNCTG 1 cut(s) 945
PsuI RGATCY 2 cut(s) 429, 695
PsyI GACNNNGTC 1 cut(s) 229
RsaI GTAC 2 cut(s) 528, 617
RsaNI GTAC 2 cut(s) 527, 616
SapI GCTCTTC 1 cut(s) 127
SaqAI TTAA 2 cut(s) 552, 585
SatI GCNGC 4 cut(s) 90, 108, 890, 940
Sau3AI GATC 7 cut(s) 113, 221, 285, 363, 429, 607, 695
SchI GAGTC 2 cut(s) 174, 525
ScrFI CCNGG 6 cut(s) 513, 700, 774, 837, 1043, 1152
SduI GDGCHC 4 cut(s) 315, 336, 472, 883
SfaNI GCATC 2 cut(s) 834, 1017
SinI GGWCC 9 cut(s) 731, 770, 776, 791, 812, 833, 860, 905, 1070
Sse9I AATT 1 cut(s) 763
SspMI CTAG 4 cut(s) 354, 389, 420, 447
StyD4I CCNGG 6 cut(s) 511, 698, 772, 835, 1041, 1150
StyI CCWWGG 2 cut(s) 45, 276
TaaI ACNGT 3 cut(s) 397, 1139, 1163
TaiI ACGT 2 cut(s) 233, 670
TaqI TCGA 5 cut(s) 224, 263, 294, 309, 646
TasI AATT 1 cut(s) 763
TauI GCSGC 3 cut(s) 92, 110, 892
TfiI GAWTC 1 cut(s) 536
Tru1I TTAA 2 cut(s) 552, 585
Tru9I TTAA 2 cut(s) 552, 585
TscAI CASTG 1 cut(s) 320
TseFI GTSAC 2 cut(s) 302, 344
TseI GCWGC 1 cut(s) 939
Tsp45I GTSAC 2 cut(s) 302, 344
TspDTI ATGAA 3 cut(s) 42, 440, 636
TspGWI ACGGA 2 cut(s) 509, 582
TspRI CASTG 1 cut(s) 320
Tth111I GACNNNGTC 1 cut(s) 229
Van91I CCANNNNNTGG 1 cut(s) 46
VpaK11BI GGWCC 9 cut(s) 731, 770, 776, 791, 812, 833, 860, 905, 1070
VspI ATTAAT 1 cut(s) 585
XapI RAATTY 1 cut(s) 763
XcmI CCANNNNNNNNNTGG 2 cut(s) 255, 942
XspI CTAG 4 cut(s) 354, 389, 420, 447
Zsp2I ATGCAT 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.