Rroxscaffold_1G00048920

protein At3g15000, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0009062
Physical Location & Seq
Reverse (-)
69030106 .. 69032568
2463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00048920.1

Sequence Viewer

Length: 1221 bp
ATGGCGACCCATTTCCTCTCTCGCTCCTTCCCCAAAACCCTAGCCCTCTCCTCCTTCCTCTCCCGCTCTCTTTCCACCGCCACCGCCACCCTCTCCTCCGCTCACTCCGCTCACTCCCGCTCCTCCCTCTCCCTTATCAACCGCCTCCGCCCCCTCGCCGCCGCCGCAATCACCGCCTTCCCCGGCAAGCTCCCTCCCGCCGCTATCCGCTGCTTCTCGGCCAACGCCACCACGTCCTCGCTCCGCGACCCCAACCCCAACTGGTCCAACCGGCCTCCCAAGGAGACTATCCTCCTCGATGGCTGCGACTTCGAGCACTGGCTCGTCGTCATGGAGCCGCCGCCGGAGGGCACCACTAGGGATGACATCATCAAGGACTACATCAAAACCCTAGCCATGGTCGTCGGAAGTGAGGAAGAAGCGAGGATGAAGATCTACTCTGTTTCAACTAGGTGTTATTATGCATTTGGTGCACTCGTGTCTGAAGAGCTTTCATACAAAATCAAGGAATTGCCAAAAGTACGCTGGGTGCTTCCTGATTCGTACCTGGATGTTAAGAACAAAGATTATGGAGGGGAACCTTTTATTAATGGGGAAGCAGTACCATATGATCCCAAGTACCATGAGGAATGGATAAGAAACAACAGTCGGGCAAACGAGAGAAACAGGCGCAATGATAGACCTAGGAATTTTGACAATTCAAGGAACTTCGACAGGAGAAGGGAAAACATGCAGCGCAGAGATGTCCAGAATAGGGGTGCCCCTCCTTTGCCTAATCAACCTGGCCAGCATGGGCCTGGCATGGCCGGACCTCCCAGCAACATGAGTCCACCCAACAATATGCCTCCCCCACCTGTGGACAGGCCTCCAATGCCTCCCAACAACACGAGTGCACCAAACTACAACAATATGCCACAGGCAAGCAACAACTGGGGCGGAGGACAGCCGAACTACAATCGGATGCCTCCAAACAGCTACAATCAGGGGTCGCCTAACAACTATAACCAGATGCCTCCAAACAACAATATGCGAGGGCCACCACCACCCCAAAACCACATGGGAGGACCGCCGCCACACATGGGAGGACCGCCACCACACATGGGAGGACAGCCACCACACATGGGAGGAGGGCAACCTGCGCCATCATGGTCTGATCCTGCACAATACCAAAATAACTACACACCAGAGAGAGATAATGGTGGTGGTCAAAACCGTTATTGA

Protein Analysis

406

Amino Acids

45.12

Weight (kDa)

9.41

Isoelectric Point (pI)

62.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MORF_dom PF21864 104 - 195 4.1e-38 Multiple organellar RNA editing factor 1, MORF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1144
AccB1I GGYRCC 2 cut(s) 350, 758
AccBSI CCGCTC 4 cut(s) 66, 101, 110, 120
AccII CGCG 1 cut(s) 246
AclWI GGATC 2 cut(s) 605, 1148
AcoI YGGCCR 3 cut(s) 219, 784, 804
AcsI RAATTY 1 cut(s) 688
AcuI CTGAAG 1 cut(s) 504
AfaI GTAC 4 cut(s) 522, 545, 603, 620
AfiI CCNNNNNNNGG 7 cut(s) 347, 397, 754, 856, 1079, 1100, 1121
AgsI TTSAA 2 cut(s) 447, 702
AjiI CACGTC 1 cut(s) 234
AjnI CCWGG 3 cut(s) 546, 781, 796
AluBI AGCT 3 cut(s) 190, 490, 975
AluI AGCT 3 cut(s) 190, 490, 975
Alw21I GWGCWC 3 cut(s) 318, 475, 895
Alw26I GTCTC 1 cut(s) 278
Alw44I GTGCAC 2 cut(s) 471, 891
AlwI GGATC 2 cut(s) 605, 1148
AoxI GGCC 7 cut(s) 219, 272, 784, 794, 804, 863, 1034
ApaLI GTGCAC 2 cut(s) 471, 891
ApeKI GCWGC 3 cut(s) 210, 303, 733
ApoI RAATTY 1 cut(s) 688
AseI ATTAAT 1 cut(s) 588
AspA2I CCTAGG 1 cut(s) 683
AspLEI GCGC 3 cut(s) 672, 738, 1141
AspS9I GGNCC 6 cut(s) 264, 794, 809, 1034, 1064, 1085
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 1 cut(s) 163
AvaII GGWCC 4 cut(s) 264, 809, 1064, 1085
AvrII CCTAGG 1 cut(s) 683
BaeGI GKGCMC 4 cut(s) 353, 475, 763, 895
BalI TGGCCA 1 cut(s) 786
BanI GGYRCC 2 cut(s) 350, 758
BauI CACGAG 2 cut(s) 476, 886
Bbv12I GWGCWC 3 cut(s) 318, 475, 895
BbvI GCAGC 3 cut(s) 197, 290, 745
BccI CCATC 2 cut(s) 293, 1150
BciT130I CCWGG 3 cut(s) 548, 783, 798
BcnI CCSGG 1 cut(s) 183
BcoDI GTCTC 1 cut(s) 278
BfaI CTAG 5 cut(s) 41, 357, 392, 450, 684
BfuAI ACCTGC 1 cut(s) 1144
BglII AGATCT 1 cut(s) 432
BlnI CCTAGG 1 cut(s) 683
Bme1390I CCNGG 4 cut(s) 183, 548, 783, 798
Bme18I GGWCC 4 cut(s) 264, 809, 1064, 1085
BmgBI CACGTC 1 cut(s) 234
BmgT120I GGNCC 6 cut(s) 264, 794, 809, 1034, 1064, 1085
BmiI GGNNCC 4 cut(s) 336, 352, 579, 760
BmrFI CCNGG 4 cut(s) 183, 548, 783, 798
BmrI ACTGGG 1 cut(s) 940
BmsI GCATC 2 cut(s) 951, 999
BmuI ACTGGG 1 cut(s) 940
BpuMI CCSGG 1 cut(s) 183
BsaBI GATNNNNATC 1 cut(s) 431
BsaJI CCNNGG 4 cut(s) 181, 279, 396, 683
BsaXI ACNNNNNCTCC 2 cut(s) 104, 134
Bsc4I CCNNNNNNNGG 7 cut(s) 347, 397, 754, 856, 1079, 1100, 1121
Bse118I RCCGGY 1 cut(s) 270
Bse1I ACTGG 3 cut(s) 266, 323, 935
Bse3DI GCAATG 1 cut(s) 679
Bse8I GATNNNNATC 1 cut(s) 431
BseBI CCWGG 3 cut(s) 548, 783, 798
BseDI CCNNGG 4 cut(s) 181, 279, 396, 683
BseGI GGATG 4 cut(s) 367, 432, 556, 966
BseJI GATNNNNATC 1 cut(s) 431
BseLI CCNNNNNNNGG 7 cut(s) 347, 397, 754, 856, 1079, 1100, 1121
BseMI GCAATG 1 cut(s) 679
BseNI ACTGG 3 cut(s) 266, 323, 935
BseRI GAGGAG 5 cut(s) 40, 85, 112, 284, 1140
BseSI GKGCMC 4 cut(s) 353, 475, 763, 895
BseXI GCAGC 3 cut(s) 197, 290, 745
BseYI CCCAGC 2 cut(s) 525, 815
BsgI GTGCAG 1 cut(s) 1143
Bsh1236I CGCG 1 cut(s) 246
BshFI GGCC 7 cut(s) 221, 274, 786, 796, 806, 865, 1036
BshNI GGYRCC 2 cut(s) 350, 758
BsiHKAI GWGCWC 3 cut(s) 318, 475, 895
BsiSI CCGG 4 cut(s) 183, 271, 344, 807
BslI CCNNNNNNNGG 7 cut(s) 347, 397, 754, 856, 1079, 1100, 1121
BsmAI GTCTC 1 cut(s) 278
BsnI GGCC 7 cut(s) 221, 274, 786, 796, 806, 865, 1036
Bsp1286I GDGCHC 5 cut(s) 318, 353, 475, 763, 895
Bsp143I GATC 3 cut(s) 432, 610, 1153
Bsp19I CCATGG 1 cut(s) 396
BspANI GGCC 7 cut(s) 221, 274, 786, 796, 806, 865, 1036
BspFNI CGCG 1 cut(s) 246
BspLI GGNNCC 4 cut(s) 336, 352, 579, 760
BspMI ACCTGC 1 cut(s) 1144
BspPI GGATC 2 cut(s) 605, 1148
BspQI GCTCTTC 1 cut(s) 480
BspT107I GGYRCC 2 cut(s) 350, 758
BsrBI CCGCTC 4 cut(s) 66, 101, 110, 120
BsrDI GCAATG 1 cut(s) 679
BsrFI RCCGGY 1 cut(s) 270
BsrI ACTGG 3 cut(s) 266, 323, 935
BssAI RCCGGY 1 cut(s) 270
BssECI CCNNGG 4 cut(s) 181, 279, 396, 683
BssMI GATC 3 cut(s) 432, 610, 1153
BssSI CACGAG 2 cut(s) 476, 886
BssT1I CCWWGG 3 cut(s) 279, 396, 683
Bst2BI CACGAG 2 cut(s) 476, 886
Bst2UI CCWGG 3 cut(s) 548, 783, 798
Bst4CI ACNGT 2 cut(s) 647, 1214
Bst6I CTCTTC 1 cut(s) 480
BstAPI GCANNNNNTGC 1 cut(s) 470
BstC8I GCNNGC 3 cut(s) 188, 788, 922
BstDSI CCRYGG 1 cut(s) 396
BstF5I GGATG 4 cut(s) 367, 432, 556, 966
BstFNI CGCG 1 cut(s) 246
BstHHI GCGC 3 cut(s) 672, 738, 1141
BstKTI GATC 3 cut(s) 435, 613, 1156
BstMAI GTCTC 1 cut(s) 278
BstMBI GATC 3 cut(s) 432, 610, 1153
BstMWI GCNNNNNNNGC 6 cut(s) 107, 164, 173, 470, 871, 1138
BstNI CCWGG 3 cut(s) 548, 783, 798
BstNSI RCATGY 1 cut(s) 733
BstSCI CCNGG 4 cut(s) 181, 546, 781, 796
BstSLI GKGCMC 4 cut(s) 353, 475, 763, 895
BstUI CGCG 1 cut(s) 246
BstV1I GCAGC 3 cut(s) 197, 290, 745
BstX2I RGATCY 1 cut(s) 432
BstYI RGATCY 1 cut(s) 432
BsuRI GGCC 7 cut(s) 221, 274, 786, 796, 806, 865, 1036
BtgI CCRYGG 1 cut(s) 396
BtrI CACGTC 1 cut(s) 234
BtsCI GGATG 4 cut(s) 367, 432, 556, 966
BtsIMutI CAGTG 1 cut(s) 316
BveI ACCTGC 1 cut(s) 1144
Cac8I GCNNGC 3 cut(s) 188, 788, 922
CfoI GCGC 3 cut(s) 672, 738, 1141
Cfr10I RCCGGY 1 cut(s) 270
Cfr13I GGNCC 6 cut(s) 264, 794, 809, 1034, 1064, 1085
Csp6I GTAC 4 cut(s) 521, 544, 602, 619
CviQI GTAC 4 cut(s) 521, 544, 602, 619
DpnI GATC 3 cut(s) 434, 612, 1155
DpnII GATC 3 cut(s) 432, 610, 1153
EaeI YGGCCR 3 cut(s) 219, 784, 804
Eam1104I CTCTTC 1 cut(s) 480
EarI CTCTTC 1 cut(s) 480
EciI GGCGGA 2 cut(s) 137, 951
Eco130I CCWWGG 3 cut(s) 279, 396, 683
Eco147I AGGCCT 1 cut(s) 865
Eco47I GGWCC 4 cut(s) 264, 809, 1064, 1085
Eco57I CTGAAG 1 cut(s) 504
EcoRII CCWGG 3 cut(s) 546, 781, 796
EcoT14I CCWWGG 3 cut(s) 279, 396, 683
EcoT22I ATGCAT 1 cut(s) 466
ErhI CCWWGG 3 cut(s) 279, 396, 683
FauI CCCGC 3 cut(s) 71, 125, 205
FauNDI CATATG 1 cut(s) 607
FokI GGATG 4 cut(s) 374, 439, 563, 973
FspBI CTAG 5 cut(s) 41, 357, 392, 450, 684
GlaI GCGC 3 cut(s) 671, 737, 1140
GsaI CCCAGC 2 cut(s) 529, 819
HaeIII GGCC 7 cut(s) 221, 274, 786, 796, 806, 865, 1036
HapII CCGG 4 cut(s) 183, 271, 344, 807
HhaI GCGC 3 cut(s) 672, 738, 1141
Hin6I GCGC 3 cut(s) 670, 736, 1139
HinP1I GCGC 3 cut(s) 670, 736, 1139
HinfI GANTC 2 cut(s) 539, 826
HpaII CCGG 4 cut(s) 183, 271, 344, 807
HphI GGTGA 1 cut(s) 163
Hpy166II GTNNAC 4 cut(s) 473, 830, 859, 893
Hpy188I TCNGA 4 cut(s) 407, 484, 960, 1153
Hpy188III TCNNGA 2 cut(s) 536, 748
Hpy8I GTNNAC 4 cut(s) 473, 830, 859, 893
Hpy99I CGWCG 2 cut(s) 329, 407
HpyAV CCTTC 4 cut(s) 37, 64, 187, 714
HpyCH4III ACNGT 2 cut(s) 647, 1214
HpyCH4IV ACGT 1 cut(s) 233
HpyCH4V TGCA 5 cut(s) 464, 473, 733, 893, 1160
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 164, 173, 470, 871, 1138
HpySE526I ACGT 1 cut(s) 233
HspAI GCGC 3 cut(s) 670, 736, 1139
Kzo9I GATC 3 cut(s) 432, 610, 1153
LguI GCTCTTC 1 cut(s) 480
LmnI GCTCC 5 cut(s) 29, 125, 195, 246, 334
Lsp1109I GCAGC 3 cut(s) 197, 290, 745
LweI GCATC 2 cut(s) 951, 999
MaeI CTAG 5 cut(s) 41, 357, 392, 450, 684
MaeII ACGT 1 cut(s) 233
MalI GATC 3 cut(s) 434, 612, 1155
MbiI CCGCTC 4 cut(s) 66, 101, 110, 120
MboI GATC 3 cut(s) 432, 610, 1153
MboII GAAGA 3 cut(s) 428, 442, 497
MflI RGATCY 1 cut(s) 432
MhlI GDGCHC 5 cut(s) 318, 353, 475, 763, 895
MlsI TGGCCA 1 cut(s) 786
MluCI AATT 3 cut(s) 509, 688, 697
MluNI TGGCCA 1 cut(s) 786
MlyI GAGTC 1 cut(s) 835
MmeI TCCRAC 2 cut(s) 291, 385
Mox20I TGGCCA 1 cut(s) 786
Mph1103I ATGCAT 1 cut(s) 466
MscI TGGCCA 1 cut(s) 786
MseI TTAA 2 cut(s) 555, 588
Msp20I TGGCCA 1 cut(s) 786
MspA1I CMGCKG 1 cut(s) 210
MspI CCGG 4 cut(s) 183, 271, 344, 807
MspR9I CCNGG 4 cut(s) 183, 548, 783, 798
MvaI CCWGG 3 cut(s) 548, 783, 798
MvnI CGCG 1 cut(s) 246
MwoI GCNNNNNNNGC 6 cut(s) 107, 164, 173, 470, 871, 1138
NciI CCSGG 1 cut(s) 183
NcoI CCATGG 1 cut(s) 396
NdeI CATATG 1 cut(s) 607
NdeII GATC 3 cut(s) 432, 610, 1153
NlaIV GGNNCC 4 cut(s) 336, 352, 579, 760
NmeAIII GCCGAG 1 cut(s) 197
NsiI ATGCAT 1 cut(s) 466
NspI RCATGY 1 cut(s) 733
PceI AGGCCT 1 cut(s) 865
PciSI GCTCTTC 1 cut(s) 480
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PfeI GAWTC 1 cut(s) 539
PleI GAGTC 1 cut(s) 834
PpsI GAGTC 1 cut(s) 834
PshBI ATTAAT 1 cut(s) 588
Psp6I CCWGG 3 cut(s) 546, 781, 796
PspFI CCCAGC 2 cut(s) 525, 815
PspGI CCWGG 3 cut(s) 546, 781, 796
PspN4I GGNNCC 4 cut(s) 336, 352, 579, 760
PspPI GGNCC 6 cut(s) 264, 794, 809, 1034, 1064, 1085
PsuI RGATCY 1 cut(s) 432
RsaI GTAC 4 cut(s) 522, 545, 603, 620
RsaNI GTAC 4 cut(s) 521, 544, 602, 619
SapI GCTCTTC 1 cut(s) 480
SaqAI TTAA 2 cut(s) 555, 588
Sau3AI GATC 3 cut(s) 432, 610, 1153
Sau96I GGNCC 6 cut(s) 264, 794, 809, 1034, 1064, 1085
SchI GAGTC 1 cut(s) 835
ScrFI CCNGG 4 cut(s) 183, 548, 783, 798
SduI GDGCHC 5 cut(s) 318, 353, 475, 763, 895
SfaNI GCATC 2 cut(s) 951, 999
SinI GGWCC 4 cut(s) 264, 809, 1064, 1085
Sse9I AATT 3 cut(s) 509, 688, 697
SseBI AGGCCT 1 cut(s) 865
SspMI CTAG 5 cut(s) 41, 357, 392, 450, 684
StuI AGGCCT 1 cut(s) 865
StyD4I CCNGG 4 cut(s) 181, 546, 781, 796
StyI CCWWGG 3 cut(s) 279, 396, 683
TaaI ACNGT 2 cut(s) 647, 1214
TaiI ACGT 1 cut(s) 236
TaqI TCGA 3 cut(s) 297, 312, 711
TasI AATT 3 cut(s) 509, 688, 697
TauI GCSGC 7 cut(s) 161, 164, 167, 203, 340, 343, 1072
TfiI GAWTC 1 cut(s) 539
Tru1I TTAA 2 cut(s) 555, 588
Tru9I TTAA 2 cut(s) 555, 588
TscAI CASTG 1 cut(s) 323
TseI GCWGC 3 cut(s) 210, 303, 733
TspDTI ATGAA 2 cut(s) 443, 483
TspRI CASTG 1 cut(s) 323
VneI GTGCAC 2 cut(s) 471, 891
VpaK11BI GGWCC 4 cut(s) 264, 809, 1064, 1085
VspI ATTAAT 1 cut(s) 588
XapI RAATTY 1 cut(s) 688
XceI RCATGY 1 cut(s) 733
XcmI CCANNNNNNNNNTGG 2 cut(s) 522, 794
XmaJI CCTAGG 1 cut(s) 683
XspI CTAG 5 cut(s) 41, 357, 392, 450, 684
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.