RLG00000033306

protein At3g15000, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0009062
Physical Location & Seq
Forward (+)
25549280 .. 25551140
1861 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033306

Sequence Viewer

Length: 849 bp
ATGGAGCCGCCGCCGGAGGGCACCACTAGGGATGACATCATCAAGGAGTACATTAAAACCCTAGCCATGGTCGTCGGAAGTGAGGAAGAAGCGAGGATGAAGATCTACTCTGTCTCAACTAGGTGTTATTTTGCATTTGGTGCACTTGTGTCTGAAGAGCTTTCATACAAAATCAAGGAATTGCCAAAGGTCCGCTGGGTGCTTCCTGATTCGTACTTGGATGTTAAGAACAAAGATTACGGAGGCGAACCTTTTATTAATGGGGAAGCAGTACCATATGATCCCAAGTACCATGAGGAATGGATAAGAAACAACAGTCGGGCAAACGAGAGAAACAGGCGCAATGATAGACCTAGGAACTTTGACAATTCAAGGAACTTTGACAGGAGAAGGGAAAACATGCAGCGCAGAGATTTCCAGGATAGGGGTGCCCCTCCTTTGCCTAATCAACCTGGCCAGCATGGGCCTGGCATGGCCGGACCTCCCAGCAACATGAGTCCACCCAACAATATGCCTCCCCCACCTGTGGACAGGCCTCCAATGCCTCCCAACAACATGAGTGCACCAAACTACAACAATATGCCACAGGCAAGCAACAACTGGGGCGGAGGACAGCCGAACTACAATCGGATGCCTCCAAACAACTACAATCAGGGGTCGCCTAACAACTATAATCAGATGCCTCCAAACAACAATATGCGAGGGCCACCACCACCCCAAAACCACATGGGAGGACCGCCACCGCACATGGGAGGAGGGCAACCTGCGCCATCATGGTCTGATCCTGCACAATACCAAAATAACTACACACCAGAGAGAGATAATGGTGGTGGTCAAAACCGTTACTGA

Protein Analysis

283

Amino Acids

32.02

Weight (kDa)

8.53

Isoelectric Point (pI)

66.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MORF_dom PF21864 1 - 85 4.2e-33 Multiple organellar RNA editing factor 1, MORF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 772
AccB1I GGYRCC 2 cut(s) 20, 428
AciI CCGC 6 cut(s) 8, 11, 193, 606, 737, 743
AclWI GGATC 2 cut(s) 275, 776
AcoI YGGCCR 2 cut(s) 454, 474
AcuI CTGAAG 1 cut(s) 174
AfaI GTAC 4 cut(s) 50, 215, 273, 290
AfiI CCNNNNNNNGG 5 cut(s) 17, 67, 424, 526, 749
AgsI TTSAA 1 cut(s) 372
AjnI CCWGG 3 cut(s) 417, 451, 466
AluBI AGCT 1 cut(s) 160
AluI AGCT 1 cut(s) 160
Alw21I GWGCWC 2 cut(s) 145, 565
Alw26I GTCTC 1 cut(s) 118
Alw44I GTGCAC 2 cut(s) 141, 561
AlwI GGATC 2 cut(s) 275, 776
AoxI GGCC 5 cut(s) 454, 464, 474, 533, 704
ApaLI GTGCAC 2 cut(s) 141, 561
ApeKI GCWGC 1 cut(s) 403
AseI ATTAAT 1 cut(s) 258
AspA2I CCTAGG 1 cut(s) 353
AspLEI GCGC 3 cut(s) 342, 408, 769
AspS9I GGNCC 5 cut(s) 190, 464, 479, 704, 734
AvaII GGWCC 3 cut(s) 190, 479, 734
AvrII CCTAGG 1 cut(s) 353
BaeGI GKGCMC 4 cut(s) 23, 145, 433, 565
BalI TGGCCA 1 cut(s) 456
BanI GGYRCC 2 cut(s) 20, 428
Bbv12I GWGCWC 2 cut(s) 145, 565
BbvI GCAGC 1 cut(s) 415
BccI CCATC 1 cut(s) 778
BciT130I CCWGG 3 cut(s) 419, 453, 468
BcoDI GTCTC 1 cut(s) 118
BfaI CTAG 4 cut(s) 27, 62, 120, 354
BfuAI ACCTGC 1 cut(s) 772
BglII AGATCT 1 cut(s) 102
BisI GCNGC 3 cut(s) 8, 11, 404
BlnI CCTAGG 1 cut(s) 353
BlsI GCNGC 3 cut(s) 9, 12, 405
Bme1390I CCNGG 3 cut(s) 419, 453, 468
Bme18I GGWCC 3 cut(s) 190, 479, 734
BmgT120I GGNCC 5 cut(s) 190, 464, 479, 704, 734
BmiI GGNNCC 3 cut(s) 6, 22, 430
BmrFI CCNGG 3 cut(s) 419, 453, 468
BmrI ACTGGG 1 cut(s) 610
BmsI GCATC 2 cut(s) 621, 669
BmuI ACTGGG 1 cut(s) 610
BsaBI GATNNNNATC 1 cut(s) 101
BsaJI CCNNGG 2 cut(s) 66, 353
Bsc4I CCNNNNNNNGG 5 cut(s) 17, 67, 424, 526, 749
Bse1I ACTGG 1 cut(s) 605
Bse3DI GCAATG 1 cut(s) 349
Bse8I GATNNNNATC 1 cut(s) 101
BseBI CCWGG 3 cut(s) 419, 453, 468
BseDI CCNNGG 2 cut(s) 66, 353
BseGI GGATG 4 cut(s) 37, 102, 226, 636
BseJI GATNNNNATC 1 cut(s) 101
BseLI CCNNNNNNNGG 5 cut(s) 17, 67, 424, 526, 749
BseMI GCAATG 1 cut(s) 349
BseNI ACTGG 1 cut(s) 605
BseRI GAGGAG 1 cut(s) 768
BseSI GKGCMC 4 cut(s) 23, 145, 433, 565
BseXI GCAGC 1 cut(s) 415
BseYI CCCAGC 2 cut(s) 195, 485
BsgI GTGCAG 1 cut(s) 771
BshFI GGCC 5 cut(s) 456, 466, 476, 535, 706
BshNI GGYRCC 2 cut(s) 20, 428
BsiHKAI GWGCWC 2 cut(s) 145, 565
BsiSI CCGG 2 cut(s) 14, 477
BslI CCNNNNNNNGG 5 cut(s) 17, 67, 424, 526, 749
BsmAI GTCTC 1 cut(s) 118
BsnI GGCC 5 cut(s) 456, 466, 476, 535, 706
Bsp1286I GDGCHC 4 cut(s) 23, 145, 433, 565
Bsp143I GATC 3 cut(s) 102, 280, 781
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 6 cut(s) 8, 11, 193, 606, 737, 743
BspANI GGCC 5 cut(s) 456, 466, 476, 535, 706
BspLI GGNNCC 3 cut(s) 6, 22, 430
BspMI ACCTGC 1 cut(s) 772
BspPI GGATC 2 cut(s) 275, 776
BspQI GCTCTTC 1 cut(s) 150
BspT107I GGYRCC 2 cut(s) 20, 428
BsrDI GCAATG 1 cut(s) 349
BsrI ACTGG 1 cut(s) 605
BssECI CCNNGG 2 cut(s) 66, 353
BssMI GATC 3 cut(s) 102, 280, 781
BssT1I CCWWGG 2 cut(s) 66, 353
Bst2UI CCWGG 3 cut(s) 419, 453, 468
Bst4CI ACNGT 2 cut(s) 317, 842
Bst6I CTCTTC 1 cut(s) 150
BstAPI GCANNNNNTGC 1 cut(s) 140
BstC8I GCNNGC 2 cut(s) 458, 592
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 4 cut(s) 37, 102, 226, 636
BstHHI GCGC 3 cut(s) 342, 408, 769
BstKTI GATC 3 cut(s) 105, 283, 784
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 3 cut(s) 102, 280, 781
BstMWI GCNNNNNNNGC 3 cut(s) 140, 541, 766
BstNI CCWGG 3 cut(s) 419, 453, 468
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 3 cut(s) 417, 451, 466
BstSLI GKGCMC 4 cut(s) 23, 145, 433, 565
BstV1I GCAGC 1 cut(s) 415
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BsuRI GGCC 5 cut(s) 456, 466, 476, 535, 706
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 4 cut(s) 37, 102, 226, 636
BveI ACCTGC 1 cut(s) 772
Cac8I GCNNGC 2 cut(s) 458, 592
CfoI GCGC 3 cut(s) 342, 408, 769
Cfr13I GGNCC 5 cut(s) 190, 464, 479, 704, 734
Csp6I GTAC 4 cut(s) 49, 214, 272, 289
CviJI RGCY 9 cut(s) 7, 65, 160, 456, 466, 476, 535, 616, 706
CviKI_1 RGCY 9 cut(s) 7, 65, 160, 456, 466, 476, 535, 616, 706
CviQI GTAC 4 cut(s) 49, 214, 272, 289
DpnI GATC 3 cut(s) 104, 282, 783
DpnII GATC 3 cut(s) 102, 280, 781
EaeI YGGCCR 2 cut(s) 454, 474
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
EciI GGCGGA 1 cut(s) 621
Eco130I CCWWGG 2 cut(s) 66, 353
Eco147I AGGCCT 1 cut(s) 535
Eco47I GGWCC 3 cut(s) 190, 479, 734
Eco57I CTGAAG 1 cut(s) 174
EcoRII CCWGG 3 cut(s) 417, 451, 466
EcoT14I CCWWGG 2 cut(s) 66, 353
ErhI CCWWGG 2 cut(s) 66, 353
FauNDI CATATG 1 cut(s) 277
Fnu4HI GCNGC 3 cut(s) 8, 11, 404
FokI GGATG 4 cut(s) 44, 109, 233, 643
Fsp4HI GCNGC 3 cut(s) 8, 11, 404
FspBI CTAG 4 cut(s) 27, 62, 120, 354
GlaI GCGC 3 cut(s) 341, 407, 768
GluI GCNGC 3 cut(s) 8, 11, 404
GsaI CCCAGC 2 cut(s) 199, 489
HaeIII GGCC 5 cut(s) 456, 466, 476, 535, 706
HapII CCGG 2 cut(s) 14, 477
HhaI GCGC 3 cut(s) 342, 408, 769
Hin6I GCGC 3 cut(s) 340, 406, 767
HinP1I GCGC 3 cut(s) 340, 406, 767
HinfI GANTC 2 cut(s) 209, 496
HpaII CCGG 2 cut(s) 14, 477
Hpy166II GTNNAC 4 cut(s) 143, 500, 529, 563
Hpy188I TCNGA 5 cut(s) 77, 154, 630, 678, 781
Hpy188III TCNNGA 1 cut(s) 206
Hpy8I GTNNAC 4 cut(s) 143, 500, 529, 563
Hpy99I CGWCG 1 cut(s) 77
HpyAV CCTTC 1 cut(s) 384
HpyCH4III ACNGT 2 cut(s) 317, 842
HpyCH4V TGCA 5 cut(s) 134, 143, 403, 563, 788
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 541, 766
HspAI GCGC 3 cut(s) 340, 406, 767
Kzo9I GATC 3 cut(s) 102, 280, 781
LguI GCTCTTC 1 cut(s) 150
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 1 cut(s) 415
LweI GCATC 2 cut(s) 621, 669
MaeI CTAG 4 cut(s) 27, 62, 120, 354
MaeIII GTNAC 1 cut(s) 842
MalI GATC 3 cut(s) 104, 282, 783
MboI GATC 3 cut(s) 102, 280, 781
MboII GAAGA 3 cut(s) 98, 112, 167
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 4 cut(s) 23, 145, 433, 565
MlsI TGGCCA 1 cut(s) 456
MluCI AATT 2 cut(s) 179, 367
MluNI TGGCCA 1 cut(s) 456
MlyI GAGTC 1 cut(s) 505
MmeI TCCRAC 1 cut(s) 55
Mox20I TGGCCA 1 cut(s) 456
MscI TGGCCA 1 cut(s) 456
MseI TTAA 3 cut(s) 54, 225, 258
Msp20I TGGCCA 1 cut(s) 456
MspA1I CMGCKG 1 cut(s) 195
MspI CCGG 2 cut(s) 14, 477
MspR9I CCNGG 3 cut(s) 419, 453, 468
MvaI CCWGG 3 cut(s) 419, 453, 468
MwoI GCNNNNNNNGC 3 cut(s) 140, 541, 766
NcoI CCATGG 1 cut(s) 66
NdeI CATATG 1 cut(s) 277
NdeII GATC 3 cut(s) 102, 280, 781
NlaIV GGNNCC 3 cut(s) 6, 22, 430
NspI RCATGY 1 cut(s) 403
PceI AGGCCT 1 cut(s) 535
PciSI GCTCTTC 1 cut(s) 150
PfeI GAWTC 1 cut(s) 209
PfoI TCCNGGA 1 cut(s) 417
PkrI GCNGC 3 cut(s) 9, 12, 405
PleI GAGTC 1 cut(s) 504
PpsI GAGTC 1 cut(s) 504
PshBI ATTAAT 1 cut(s) 258
Psp6I CCWGG 3 cut(s) 417, 451, 466
PspFI CCCAGC 2 cut(s) 195, 485
PspGI CCWGG 3 cut(s) 417, 451, 466
PspN4I GGNNCC 3 cut(s) 6, 22, 430
PspPI GGNCC 5 cut(s) 190, 464, 479, 704, 734
PsrI GAACNNNNNNTAC 2 cut(s) 221, 253
PsuI RGATCY 1 cut(s) 102
RsaI GTAC 4 cut(s) 50, 215, 273, 290
RsaNI GTAC 4 cut(s) 49, 214, 272, 289
SapI GCTCTTC 1 cut(s) 150
SaqAI TTAA 3 cut(s) 54, 225, 258
SatI GCNGC 3 cut(s) 8, 11, 404
Sau3AI GATC 3 cut(s) 102, 280, 781
Sau96I GGNCC 5 cut(s) 190, 464, 479, 704, 734
SchI GAGTC 1 cut(s) 505
ScrFI CCNGG 3 cut(s) 419, 453, 468
SduI GDGCHC 4 cut(s) 23, 145, 433, 565
SetI ASST 9 cut(s) 125, 162, 192, 253, 355, 454, 484, 526, 766
SfaNI GCATC 2 cut(s) 621, 669
SinI GGWCC 3 cut(s) 190, 479, 734
Sse9I AATT 2 cut(s) 179, 367
SseBI AGGCCT 1 cut(s) 535
SsiI CCGC 6 cut(s) 8, 11, 193, 606, 737, 743
SspMI CTAG 4 cut(s) 27, 62, 120, 354
StuI AGGCCT 1 cut(s) 535
StyD4I CCNGG 3 cut(s) 417, 451, 466
StyI CCWWGG 2 cut(s) 66, 353
TaaI ACNGT 2 cut(s) 317, 842
TasI AATT 2 cut(s) 179, 367
TatI WGTACW 1 cut(s) 48
TauI GCSGC 2 cut(s) 10, 13
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 3 cut(s) 54, 225, 258
Tru9I TTAA 3 cut(s) 54, 225, 258
TseI GCWGC 1 cut(s) 403
TspDTI ATGAA 2 cut(s) 113, 153
TspGWI ACGGA 1 cut(s) 255
VneI GTGCAC 2 cut(s) 141, 561
VpaK11BI GGWCC 3 cut(s) 190, 479, 734
VspI ATTAAT 1 cut(s) 258
XceI RCATGY 1 cut(s) 403
XcmI CCANNNNNNNNNTGG 2 cut(s) 192, 464
XmaJI CCTAGG 1 cut(s) 353
XspI CTAG 4 cut(s) 27, 62, 120, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.