MD12G1012700.v1.1
MYB Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004859
Physical Location & Seq
Reverse (-)
1356134 .. 1358815
2682 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1012700.v1.1.491

Sequence Viewer

Length: 1206 bp
ATGGCGTTTCGACATCTGATGAGTGATTGTGATGGTTTTACCAGATACCAAGATTTGAACTTTTTGCCACCACCTCCTGTTTCTAATCAACTTTCTCTCTCAAACATCTTTGGTGGTTCTTCTACTTCTGCAATGGGGGTTGTGCCTTTCAGTCCCTCATCATCCCAACAAACCAACAAACCATGGAGTTTTCAGGCTTTGGAAAATGGGGGACCAAAAAATATAAGTGATCTTGGTGTGCTTTCTGGGGTTGATCAGAAAAGACCCACGCCTTTGAATTTGAATCTTGTTGGAGAGGAAGATGATGATGAAGGTAGGAGTCCTGCTGGTGGGAGAACTAGTGCTAAGAGTTGTATCAGAGGCCATTGGAGACCAGCTGAAGATTCAAAGCTCAAAGAACTTGTGGGACAATATGGTCCTCAAAACTGGAACGTTATAGCTGAGCATCTTGATGGTAGATCAGGGAAGAGTTGCAGATTGAGGTGGTTCAACCAGCTAGATCCAAGGATCAACAGAGGGGCATTCAGTGAGGAGGAAGAAGAAAGGCTGCTGGCAGCCCACAGACTCTATGGCAACAAATGGGCAATGATTGCAAGGCTTTTCCCTGGAAGGACTGACAATGCTGTGAAGAACCATTGGCATGTGATCATGGCCAGAAGACACAGAGAGCAATCCAATGTTTTCAAGAGGAGAAAGCCCTCTTCTCCTCCACCTCCTTCCCCTCTTCCTCATGTGGTTGCTAATTTCCCAAAGAATCCTTCCACCAGTACTGAATCAACCGTCTCAATAAGCACCATCAATGATGACAGCACTCAGTCTGCCTCAACATGCACTGATCTCTCCCTCACTCCCTCAAACAAACCCCCTCCCACCTTTTTCTCCAAGTTCCACCCTTTTCATCGCCCCCAAATCATTGGATCACCTATGGGCCCATCATCAAGTGATGGGGGATTTAACAAGATGATTGGTAGCAATGGTAATGGTTTCTGCAAGAGCACTGGGCCAGTGAGAGGAGTTGGAGCAGCAGCTGCAGCTATGAAGGGTGTGGACAATTCCGGTCATCAATCAGATTCAGAAATTTCTGGGGACGAGTCAGTCACCACCACTAGGACCAATCTGTCATTATCTGGGGAAGATCAAGATCTGCATCATGAGAAGACCACCACCATCCATTTCATTGATTTCCTTGGAGTAGGCACTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000987 GO:0000988 GO:0000989 GO:0001012 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0001935 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006357 GO:0006950 GO:0006952 GO:0006970 GO:0007275 GO:0008150 GO:0008283 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009737 GO:0009739 GO:0009741 GO:0009751 GO:0009753 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0023051 GO:0023056 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035670 GO:0040008 GO:0042221 GO:0042493 GO:0042742 GO:0042802 GO:0042803 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045935 GO:0046677 GO:0046686 GO:0046983 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048532 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048585 GO:0048586 GO:0048608 GO:0048638 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050673 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051302 GO:0051704 GO:0051707 GO:0051716 GO:0051782 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071396 GO:0071407 GO:0071495 GO:0080050 GO:0080060 GO:0080090 GO:0080113 GO:0090567 GO:0097159 GO:0097305 GO:0098542 GO:0099402 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1904961 GO:1990837 GO:2000022 GO:2000026 GO:2000028 GO:2000031 GO:2000112 GO:2000241 GO:2000242 GO:2001023 GO:2001038 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

402

Amino Acids

43.62

Weight (kDa)

8.25

Isoelectric Point (pI)

58.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 120 - 166 2.9e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 123 - 183 6.6e-18 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 172 - 214 1.8e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 175 - 223 5.6e-09 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 414, 1094, 1117
AclI AACGTT 1 cut(s) 432
AclWI GGATC 3 cut(s) 494, 515, 925
AcoI YGGCCR 1 cut(s) 651
AcsI RAATTY 2 cut(s) 277, 1077
AcuI CTGAAG 1 cut(s) 399
AfaI GTAC 1 cut(s) 769
AfiI CCNNNNNNNGG 3 cut(s) 329, 1010, 1107
AgsI TTSAA 6 cut(s) 58, 277, 283, 387, 490, 685
AhlI ACTAGT 1 cut(s) 338
AjnI CCWGG 1 cut(s) 604
AjuI GAANNNNNNNTTGG 2 cut(s) 742, 774
AluBI AGCT 6 cut(s) 377, 391, 440, 496, 1028, 1034
AluI AGCT 6 cut(s) 377, 391, 440, 496, 1028, 1034
Alw21I GWGCWC 1 cut(s) 998
Alw26I GTCTC 2 cut(s) 364, 787
AlwI GGATC 3 cut(s) 494, 515, 925
AlwNI CAGNNNCTG 1 cut(s) 1028
AoxI GGCC 4 cut(s) 361, 651, 928, 1001
ApaI GGGCCC 1 cut(s) 932
ApeKI GCWGC 6 cut(s) 547, 554, 1022, 1025, 1028, 1031
ApoI RAATTY 2 cut(s) 277, 1077
AspS9I GGNCC 6 cut(s) 212, 416, 928, 929, 1001, 1110
AsuHPI GGTGA 2 cut(s) 912, 1090
AvaII GGWCC 3 cut(s) 212, 416, 1110
BaeGI GKGCMC 1 cut(s) 932
BalI TGGCCA 1 cut(s) 653
BanII GRGCYC 1 cut(s) 932
BbsI GAAGAC 2 cut(s) 664, 1163
Bbv12I GWGCWC 1 cut(s) 998
BbvI GCAGC 6 cut(s) 534, 566, 1015, 1034, 1037, 1043
BccI CCATC 6 cut(s) 26, 446, 803, 938, 940, 1175
BciT130I CCWGG 1 cut(s) 606
BclI TGATCA 2 cut(s) 253, 645
BcoDI GTCTC 2 cut(s) 364, 787
BcuI ACTAGT 1 cut(s) 338
BfaI CTAG 3 cut(s) 339, 497, 1107
BfmI CTRYAG 1 cut(s) 1029
BglII AGATCT 1 cut(s) 1141
BisI GCNGC 6 cut(s) 548, 555, 1023, 1026, 1029, 1032
BlpI GCTNAGC 1 cut(s) 441
BlsI GCNGC 6 cut(s) 549, 556, 1024, 1027, 1030, 1033
BmcAI AGTACT 1 cut(s) 769
Bme1390I CCNGG 1 cut(s) 606
Bme18I GGWCC 3 cut(s) 212, 416, 1110
BmgT120I GGNCC 6 cut(s) 212, 416, 928, 929, 1001, 1110
BmiI GGNNCC 2 cut(s) 213, 930
BmrFI CCNGG 1 cut(s) 606
BmrI ACTGGG 1 cut(s) 1008
BmsI GCATC 2 cut(s) 454, 1156
BmuI ACTGGG 1 cut(s) 1008
BpiI GAAGAC 2 cut(s) 664, 1163
Bpu1102I GCTNAGC 1 cut(s) 441
BsaBI GATNNNNATC 2 cut(s) 1140, 1146
BsaI GGTCTC 1 cut(s) 364
BsaJI CCNNGG 4 cut(s) 182, 503, 604, 1186
BsaWI WCCGGW 1 cut(s) 1055
BsaXI ACNNNNNCTCC 2 cut(s) 325, 355
Bsc4I CCNNNNNNNGG 3 cut(s) 329, 1010, 1107
Bse1I ACTGG 4 cut(s) 431, 765, 1003, 1004
Bse3DI GCAATG 3 cut(s) 138, 591, 979
Bse8I GATNNNNATC 2 cut(s) 1140, 1146
BseBI CCWGG 1 cut(s) 606
BseDI CCNNGG 4 cut(s) 182, 503, 604, 1186
BseGI GGATG 2 cut(s) 161, 1167
BseJI GATNNNNATC 2 cut(s) 1140, 1146
BseLI CCNNNNNNNGG 3 cut(s) 329, 1010, 1107
BseMI GCAATG 3 cut(s) 138, 591, 979
BseMII CTCAG 2 cut(s) 432, 827
BseNI ACTGG 4 cut(s) 431, 765, 1003, 1004
BseRI GAGGAG 4 cut(s) 545, 696, 703, 1026
BseSI GKGCMC 1 cut(s) 932
BseXI GCAGC 6 cut(s) 534, 566, 1015, 1034, 1037, 1043
BshFI GGCC 4 cut(s) 363, 653, 930, 1003
BsiHKAI GWGCWC 1 cut(s) 998
BsiSI CCGG 1 cut(s) 1056
BslFI GGGAC 4 cut(s) 138, 225, 420, 1100
BslI CCNNNNNNNGG 3 cut(s) 329, 1010, 1107
BsmAI GTCTC 2 cut(s) 364, 787
BsmBI CGTCTC 1 cut(s) 787
BsmFI GGGAC 4 cut(s) 138, 225, 420, 1100
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 4 cut(s) 363, 653, 930, 1003
Bso31I GGTCTC 1 cut(s) 364
Bsp120I GGGCCC 1 cut(s) 928
Bsp1286I GDGCHC 2 cut(s) 932, 998
Bsp1720I GCTNAGC 1 cut(s) 441
Bsp19I CCATGG 1 cut(s) 182
BspANI GGCC 4 cut(s) 363, 653, 930, 1003
BspCNI CTCAG 2 cut(s) 433, 826
BspHI TCATGA 1 cut(s) 1150
BspLI GGNNCC 2 cut(s) 213, 930
BspMAI CTGCAG 1 cut(s) 1033
BspPI GGATC 3 cut(s) 494, 515, 925
BspTNI GGTCTC 1 cut(s) 364
BsrDI GCAATG 3 cut(s) 138, 591, 979
BsrI ACTGG 4 cut(s) 431, 765, 1003, 1004
BssECI CCNNGG 4 cut(s) 182, 503, 604, 1186
BssT1I CCWWGG 3 cut(s) 182, 503, 1186
Bst2UI CCWGG 1 cut(s) 606
Bst4CI ACNGT 1 cut(s) 781
Bst6I CTCTTC 3 cut(s) 461, 706, 729
BstAPI GCANNNNNTGC 2 cut(s) 590, 1028
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 3 cut(s) 345, 441, 813
BstDSI CCRYGG 1 cut(s) 182
BstF5I GGATG 2 cut(s) 161, 1167
BstMAI GTCTC 2 cut(s) 364, 787
BstMWI GCNNNNNNNGC 3 cut(s) 590, 1028, 1031
BstNI CCWGG 1 cut(s) 606
BstNSI RCATGY 2 cut(s) 644, 831
BstSCI CCNGG 1 cut(s) 604
BstSFI CTRYAG 1 cut(s) 1029
BstSLI GKGCMC 1 cut(s) 932
BstV1I GCAGC 6 cut(s) 534, 566, 1015, 1034, 1037, 1043
BstV2I GAAGAC 2 cut(s) 664, 1163
BstX2I RGATCY 2 cut(s) 499, 1141
BstXI CCANNNNNNTGG 1 cut(s) 914
BstYI RGATCY 2 cut(s) 499, 1141
BsuRI GGCC 4 cut(s) 363, 653, 930, 1003
BtgI CCRYGG 1 cut(s) 182
BtgZI GCGATG 1 cut(s) 884
BtsCI GGATG 2 cut(s) 161, 1167
BtsI GCAGTG 1 cut(s) 1197
BtsIMutI CAGTG 5 cut(s) 532, 831, 996, 1011, 1197
Cac8I GCNNGC 1 cut(s) 552
CaiI CAGNNNCTG 1 cut(s) 1028
CciI TCATGA 1 cut(s) 1150
Cfr13I GGNCC 6 cut(s) 212, 416, 928, 929, 1001, 1110
Csp6I GTAC 1 cut(s) 768
CviAII CATG 6 cut(s) 183, 641, 649, 731, 828, 1151
CviQI GTAC 1 cut(s) 768
DdeI CTNAG 3 cut(s) 345, 441, 813
DrdI GACNNNNNNGTC 3 cut(s) 414, 1094, 1117
DseDI GACNNNNNNGTC 3 cut(s) 414, 1094, 1117
EaeI YGGCCR 1 cut(s) 651
Eam1104I CTCTTC 3 cut(s) 461, 706, 729
EarI CTCTTC 3 cut(s) 461, 706, 729
Eco130I CCWWGG 3 cut(s) 182, 503, 1186
Eco24I GRGCYC 1 cut(s) 932
Eco31I GGTCTC 1 cut(s) 364
Eco47I GGWCC 3 cut(s) 212, 416, 1110
Eco57I CTGAAG 1 cut(s) 399
EcoRII CCWGG 1 cut(s) 604
EcoT14I CCWWGG 3 cut(s) 182, 503, 1186
EcoT38I GRGCYC 1 cut(s) 932
ErhI CCWWGG 3 cut(s) 182, 503, 1186
Esp3I CGTCTC 1 cut(s) 787
FaeI CATG 6 cut(s) 186, 644, 652, 734, 831, 1154
FaqI GGGAC 4 cut(s) 138, 225, 420, 1100
FatI CATG 6 cut(s) 182, 640, 648, 730, 827, 1150
FbaI TGATCA 2 cut(s) 253, 645
Fnu4HI GCNGC 6 cut(s) 548, 555, 1023, 1026, 1029, 1032
FokI GGATG 2 cut(s) 148, 1154
FriOI GRGCYC 1 cut(s) 932
Fsp4HI GCNGC 6 cut(s) 548, 555, 1023, 1026, 1029, 1032
FspBI CTAG 3 cut(s) 339, 497, 1107
GluI GCNGC 6 cut(s) 548, 555, 1023, 1026, 1029, 1032
HaeIII GGCC 4 cut(s) 363, 653, 930, 1003
HapII CCGG 1 cut(s) 1056
Hin1II CATG 6 cut(s) 186, 644, 652, 734, 831, 1154
HinfI GANTC 8 cut(s) 283, 319, 383, 564, 754, 773, 1070, 1091
HpaII CCGG 1 cut(s) 1056
HphI GGTGA 2 cut(s) 912, 1090
Hpy166II GTNNAC 1 cut(s) 1048
Hpy188I TCNGA 5 cut(s) 18, 258, 359, 1069, 1075
Hpy188III TCNNGA 4 cut(s) 449, 685, 1139, 1151
Hpy8I GTNNAC 1 cut(s) 1048
HpyAV CCTTC 5 cut(s) 305, 603, 726, 768, 1033
HpyCH4III ACNGT 1 cut(s) 781
HpyCH4IV ACGT 1 cut(s) 432
HpyCH4V TGCA 7 cut(s) 131, 474, 593, 831, 990, 1031, 1147
HpyF10VI GCNNNNNNNGC 3 cut(s) 590, 1028, 1031
HpyF3I CTNAG 3 cut(s) 345, 441, 813
HpySE526I ACGT 1 cut(s) 432
Hsp92II CATG 6 cut(s) 186, 644, 652, 734, 831, 1154
Ksp22I TGATCA 2 cut(s) 253, 645
LmnI GCTCC 1 cut(s) 1019
Lsp1109I GCAGC 6 cut(s) 534, 566, 1015, 1034, 1037, 1043
LweI GCATC 2 cut(s) 454, 1156
MaeI CTAG 3 cut(s) 339, 497, 1107
MaeII ACGT 1 cut(s) 432
MaeIII GTNAC 1 cut(s) 1096
MflI RGATCY 2 cut(s) 499, 1141
MhlI GDGCHC 2 cut(s) 932, 998
MlsI TGGCCA 1 cut(s) 653
MluCI AATT 4 cut(s) 277, 742, 1051, 1077
MluNI TGGCCA 1 cut(s) 653
MlyI GAGTC 3 cut(s) 328, 558, 1100
MmeI TCCRAC 2 cut(s) 271, 997
Mox20I TGGCCA 1 cut(s) 653
MscI TGGCCA 1 cut(s) 653
MseI TTAA 2 cut(s) 954, 1204
MslI CAYNNNNRTG 2 cut(s) 450, 639
Msp20I TGGCCA 1 cut(s) 653
MspA1I CMGCKG 2 cut(s) 377, 1028
MspI CCGG 1 cut(s) 1056
MspR9I CCNGG 1 cut(s) 606
Mva1269I GAATGC 1 cut(s) 521
MvaI CCWGG 1 cut(s) 606
MwoI GCNNNNNNNGC 3 cut(s) 590, 1028, 1031
NcoI CCATGG 1 cut(s) 182
NlaIII CATG 6 cut(s) 186, 644, 652, 734, 831, 1154
NlaIV GGNNCC 2 cut(s) 213, 930
NmuCI GTSAC 1 cut(s) 1096
NspI RCATGY 2 cut(s) 644, 831
PagI TCATGA 1 cut(s) 1150
PctI GAATGC 1 cut(s) 521
PfeI GAWTC 5 cut(s) 283, 383, 754, 773, 1070
PkrI GCNGC 6 cut(s) 549, 556, 1024, 1027, 1030, 1033
PleI GAGTC 3 cut(s) 327, 558, 1099
PpsI GAGTC 3 cut(s) 327, 558, 1099
Psp1406I AACGTT 1 cut(s) 432
Psp6I CCWGG 1 cut(s) 604
PspGI CCWGG 1 cut(s) 604
PspN4I GGNNCC 2 cut(s) 213, 930
PspOMI GGGCCC 1 cut(s) 928
PspPI GGNCC 6 cut(s) 212, 416, 928, 929, 1001, 1110
PstI CTGCAG 1 cut(s) 1033
PstNI CAGNNNCTG 1 cut(s) 1028
PsuI RGATCY 2 cut(s) 499, 1141
PvuII CAGCTG 2 cut(s) 377, 1028
RsaI GTAC 1 cut(s) 769
RsaNI GTAC 1 cut(s) 768
RseI CAYNNNNRTG 2 cut(s) 450, 639
SaqAI TTAA 2 cut(s) 954, 1204
SatI GCNGC 6 cut(s) 548, 555, 1023, 1026, 1029, 1032
Sau96I GGNCC 6 cut(s) 212, 416, 928, 929, 1001, 1110
ScaI AGTACT 1 cut(s) 769
SchI GAGTC 3 cut(s) 328, 558, 1100
ScrFI CCNGG 1 cut(s) 606
SduI GDGCHC 2 cut(s) 932, 998
SfaNI GCATC 2 cut(s) 454, 1156
SfcI CTRYAG 1 cut(s) 1029
SinI GGWCC 3 cut(s) 212, 416, 1110
SmiMI CAYNNNNRTG 2 cut(s) 450, 639
SpeI ACTAGT 1 cut(s) 338
Sse9I AATT 4 cut(s) 277, 742, 1051, 1077
SspMI CTAG 3 cut(s) 339, 497, 1107
StyD4I CCNGG 1 cut(s) 604
StyI CCWWGG 3 cut(s) 182, 503, 1186
TaaI ACNGT 1 cut(s) 781
TaiI ACGT 1 cut(s) 435
TaqI TCGA 1 cut(s) 10
TasI AATT 4 cut(s) 277, 742, 1051, 1077
TatI WGTACW 1 cut(s) 767
TfiI GAWTC 5 cut(s) 283, 383, 754, 773, 1070
Tru1I TTAA 2 cut(s) 954, 1204
Tru9I TTAA 2 cut(s) 954, 1204
TscAI CASTG 5 cut(s) 532, 838, 1003, 1011, 1204
TseFI GTSAC 1 cut(s) 1096
TseI GCWGC 6 cut(s) 547, 554, 1022, 1025, 1028, 1031
Tsp45I GTSAC 1 cut(s) 1096
TspDTI ATGAA 4 cut(s) 324, 887, 1052, 1165
TspRI CASTG 5 cut(s) 532, 838, 1003, 1011, 1204
VpaK11BI GGWCC 3 cut(s) 212, 416, 1110
XapI RAATTY 2 cut(s) 277, 1077
XceI RCATGY 2 cut(s) 644, 831
XcmI CCANNNNNNNNNTGG 1 cut(s) 566
XspI CTAG 3 cut(s) 339, 497, 1107
ZrmI AGTACT 1 cut(s) 769
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.