MD14G1010600.v1.1
MYB Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004859
Physical Location & Seq
Reverse (-)
1029211 .. 1031838
2628 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1010600.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGGGTTTTCAACATCTGAAGAGTGATTGTAATGGTTTCACCAGATACCAAGATTTGAACTTTTCGCCACCACCTCCTGTTTCTAATCAACTTTCTCTCTCAAACACCTTTGGTGGTTCTTCTACTTCTGCAATGGGGCTTGGGCCTTTCAGTCCCTTATCATCCCAGCAAACCAACAAACCATGGAGTTCTCAAGCTTTGGAAAATGGGGGACCAAAAAATATAAGCGATCATGGTGTGCTTTTTGGGGTTGATCTGAAAAGGCCCGTGCCTTTGAATTTGAATCTTGTTGGAGAGGAAGATGATGATGAAGATAGGAGCCCTGCTGGTGGGAGAATTAGTGCTAAGAGTTGTGCCAGAGGCCATTGGAGACCAGCTGAAGACTCAAAGCTCAAAGAACTTGTGGGACAATATGGTCCTCAAAACTGGAACATAATAGCTGAGAAACTTGATGGTAGATCAGGGAAGAGTTGCAGATTGAGGTGGTTCAACCAGCTAGATCCAAGGATCAACAAAAGGGCATTCAGTGAGGAGGAAGAAGAGAGGCTGTTGGTTGCCCACAGACTCTATGGCAACAAATGGGCAATGATTGCAAGGCTCTTCCCTGGAAGGACTGACAATGCTGTGAAGAACCATTGGCATGTGATCATGGCCAGAAAACACCGAGAGCAATCCAATGTTTTCAAGAGGAGAAAGCCCTCTTCTCCTCCACCTCCATCTCCTCCATCACCTCCTCCTCATGTGGTCGCTAATTTCCCAAAAAATCCTTCCACCAGTACTGAATCAACCATCTCAATAAGTACCATCAATGATGACAGCACTCAGTCTGCCTCAACCTGCACTGATCTCTCCCTCACTCCCTCAAACAAACCACCTCCCACCTTTTTCACCAAGTTCCATCCTTTTCATCACCACCAAAACTTTGGATCACATTTAGGCTCATCATCAAGTGATGGGGGATTTAAAAAGATGATTGGTAGCAATGGGAATGGTTTCTGCAGGAGCACTGGGCCAGTGAGAGGAGTGGGAGCAGCAGCTGCAGCTATGATGGGTGTGGATAATTCTGGGCATCAATCAGATTCAAATTCAGATATTTCTGGGGATGAGTCAGTCACCACCACCAGGACCAATCTGTCCTTATCTGGGGAAAATCAAAATCTGCATCATGAGAAGATCTCCAGCATCCATTTCATTGATTTCCTTGGGGTAGGCACTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000987 GO:0000988 GO:0000989 GO:0001012 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0001935 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006355 GO:0006357 GO:0006950 GO:0006952 GO:0006970 GO:0007275 GO:0008150 GO:0008283 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009737 GO:0009739 GO:0009741 GO:0009751 GO:0009753 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009908 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0019219 GO:0019222 GO:0022414 GO:0023051 GO:0023056 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035670 GO:0040008 GO:0042221 GO:0042493 GO:0042742 GO:0042802 GO:0042803 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045935 GO:0046677 GO:0046686 GO:0046983 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048532 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048585 GO:0048586 GO:0048608 GO:0048638 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050673 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051173 GO:0051239 GO:0051241 GO:0051252 GO:0051254 GO:0051302 GO:0051704 GO:0051707 GO:0051716 GO:0051782 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071396 GO:0071407 GO:0071495 GO:0080050 GO:0080060 GO:0080090 GO:0080113 GO:0090567 GO:0097159 GO:0097305 GO:0098542 GO:0099402 GO:0140110 GO:1901363 GO:1901698 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:1904961 GO:1990837 GO:2000022 GO:2000026 GO:2000028 GO:2000031 GO:2000112 GO:2000241 GO:2000242 GO:2001023 GO:2001038 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

407

Amino Acids

44.24

Weight (kDa)

8.91

Isoelectric Point (pI)

54.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 120 - 166 1.5e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 123 - 183 1.5e-16 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 172 - 214 2.7e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 175 - 218 6.2e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 414, 1132
Acc36I ACCTGC 1 cut(s) 845
AclWI GGATC 3 cut(s) 494, 515, 934
AcoI YGGCCR 1 cut(s) 651
AcsI RAATTY 2 cut(s) 277, 1084
AcuI CTGAAG 2 cut(s) 38, 399
AfaI GTAC 2 cut(s) 778, 802
AfiI CCNNNNNNNGG 4 cut(s) 329, 1019, 1122, 1143
AgsI TTSAA 7 cut(s) 11, 58, 277, 283, 490, 685, 1083
AjnI CCWGG 2 cut(s) 604, 1121
AjuI GAANNNNNNNTTGG 2 cut(s) 751, 783
AluBI AGCT 7 cut(s) 197, 377, 391, 440, 496, 1037, 1043
AluI AGCT 7 cut(s) 197, 377, 391, 440, 496, 1037, 1043
Alw21I GWGCWC 1 cut(s) 1007
Alw26I GTCTC 1 cut(s) 364
AlwI GGATC 3 cut(s) 494, 515, 934
AlwNI CAGNNNCTG 1 cut(s) 1037
AoxI GGCC 5 cut(s) 143, 263, 361, 651, 1010
ApeKI GCWGC 4 cut(s) 1031, 1034, 1037, 1040
ApoI RAATTY 2 cut(s) 277, 1084
Asp700I GAANNNNTTC 1 cut(s) 992
AspS9I GGNCC 6 cut(s) 143, 212, 264, 416, 1010, 1125
AsuHPI GGTGA 5 cut(s) 31, 720, 880, 902, 1105
AvaII GGWCC 3 cut(s) 212, 416, 1125
BalI TGGCCA 1 cut(s) 653
BanII GRGCYC 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 1007
BbvI GCAGC 4 cut(s) 1024, 1043, 1046, 1052
BccI CCATC 8 cut(s) 446, 724, 733, 797, 812, 906, 947, 1042
BciT130I CCWGG 2 cut(s) 606, 1123
BclI TGATCA 1 cut(s) 645
BcoDI GTCTC 1 cut(s) 364
BfaI CTAG 1 cut(s) 497
BfmI CTRYAG 2 cut(s) 997, 1038
BfuAI ACCTGC 1 cut(s) 845
BglII AGATCT 1 cut(s) 1173
BisI GCNGC 4 cut(s) 1032, 1035, 1038, 1041
BlsI GCNGC 4 cut(s) 1033, 1036, 1039, 1042
BmcAI AGTACT 1 cut(s) 778
Bme1390I CCNGG 2 cut(s) 606, 1123
Bme18I GGWCC 3 cut(s) 212, 416, 1125
BmgT120I GGNCC 6 cut(s) 143, 212, 264, 416, 1010, 1125
BmiI GGNNCC 2 cut(s) 213, 320
BmrFI CCNGG 2 cut(s) 606, 1123
BmrI ACTGGG 1 cut(s) 1017
BmsI GCATC 3 cut(s) 1078, 1171, 1191
BmuI ACTGGG 1 cut(s) 1017
BpiI GAAGAC 1 cut(s) 387
BplI GAGNNNNNCTC 2 cut(s) 1160, 1192
BpmI CTGGAG 1 cut(s) 1162
BpuEI CTTGAG 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 364
BsaJI CCNNGG 4 cut(s) 182, 503, 604, 1201
BsaXI ACNNNNNCTCC 2 cut(s) 325, 355
Bsc4I CCNNNNNNNGG 4 cut(s) 329, 1019, 1122, 1143
Bse1I ACTGG 4 cut(s) 431, 774, 1012, 1013
Bse3DI GCAATG 3 cut(s) 138, 591, 988
BseBI CCWGG 2 cut(s) 606, 1123
BseDI CCNNGG 4 cut(s) 182, 503, 604, 1201
BseGI GGATG 4 cut(s) 161, 898, 1108, 1182
BseLI CCNNNNNNNGG 4 cut(s) 329, 1019, 1122, 1143
BseMI GCAATG 3 cut(s) 138, 591, 988
BseMII CTCAG 2 cut(s) 432, 836
BseNI ACTGG 4 cut(s) 431, 774, 1012, 1013
BseRI GAGGAG 7 cut(s) 545, 696, 703, 711, 723, 726, 1035
BseXI GCAGC 4 cut(s) 1024, 1043, 1046, 1052
BseYI CCCAGC 1 cut(s) 165
BsgI GTGCAG 1 cut(s) 823
BshFI GGCC 5 cut(s) 145, 265, 363, 653, 1012
BsiHKAI GWGCWC 1 cut(s) 1007
BslFI GGGAC 3 cut(s) 138, 225, 420
BslI CCNNNNNNNGG 4 cut(s) 329, 1019, 1122, 1143
BsmAI GTCTC 1 cut(s) 364
BsmFI GGGAC 3 cut(s) 138, 225, 420
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 5 cut(s) 145, 265, 363, 653, 1012
Bso31I GGTCTC 1 cut(s) 364
Bsp1286I GDGCHC 2 cut(s) 323, 1007
Bsp143I GATC 9 cut(s) 229, 253, 458, 499, 507, 645, 844, 926, 1173
Bsp19I CCATGG 1 cut(s) 182
BspANI GGCC 5 cut(s) 145, 265, 363, 653, 1012
BspCNI CTCAG 2 cut(s) 433, 835
BspHI TCATGA 1 cut(s) 1165
BspLI GGNNCC 2 cut(s) 213, 320
BspMAI CTGCAG 2 cut(s) 1001, 1042
BspMI ACCTGC 1 cut(s) 845
BspPI GGATC 3 cut(s) 494, 515, 934
BspQI GCTCTTC 1 cut(s) 605
BspTNI GGTCTC 1 cut(s) 364
BsrDI GCAATG 3 cut(s) 138, 591, 988
BsrI ACTGG 4 cut(s) 431, 774, 1012, 1013
BssECI CCNNGG 4 cut(s) 182, 503, 604, 1201
BssMI GATC 9 cut(s) 229, 253, 458, 499, 507, 645, 844, 926, 1173
BssT1I CCWWGG 3 cut(s) 182, 503, 1201
Bst2UI CCWGG 2 cut(s) 606, 1123
Bst6I CTCTTC 5 cut(s) 14, 461, 534, 605, 706
BstAPI GCANNNNNTGC 2 cut(s) 590, 1037
BstDEI CTNAG 3 cut(s) 345, 441, 822
BstDSI CCRYGG 1 cut(s) 182
BstF5I GGATG 4 cut(s) 161, 898, 1108, 1182
BstKTI GATC 9 cut(s) 232, 256, 461, 502, 510, 648, 847, 929, 1176
BstMAI GTCTC 1 cut(s) 364
BstMBI GATC 9 cut(s) 229, 253, 458, 499, 507, 645, 844, 926, 1173
BstMWI GCNNNNNNNGC 3 cut(s) 590, 1037, 1040
BstNI CCWGG 2 cut(s) 606, 1123
BstNSI RCATGY 1 cut(s) 644
BstSCI CCNGG 2 cut(s) 604, 1121
BstSFI CTRYAG 2 cut(s) 997, 1038
BstV1I GCAGC 4 cut(s) 1024, 1043, 1046, 1052
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 2 cut(s) 499, 1173
BstXI CCANNNNNNTGG 1 cut(s) 923
BstYI RGATCY 2 cut(s) 499, 1173
BsuRI GGCC 5 cut(s) 145, 265, 363, 653, 1012
BtgI CCRYGG 1 cut(s) 182
BtsCI GGATG 4 cut(s) 161, 898, 1108, 1182
BtsIMutI CAGTG 4 cut(s) 532, 840, 1005, 1020
BveI ACCTGC 1 cut(s) 845
CaiI CAGNNNCTG 1 cut(s) 1037
CciI TCATGA 1 cut(s) 1165
Cfr13I GGNCC 6 cut(s) 143, 212, 264, 416, 1010, 1125
Csp6I GTAC 2 cut(s) 777, 801
CviAII CATG 6 cut(s) 183, 233, 641, 649, 740, 1166
CviQI GTAC 2 cut(s) 777, 801
DdeI CTNAG 3 cut(s) 345, 441, 822
DpnI GATC 9 cut(s) 231, 255, 460, 501, 509, 647, 846, 928, 1175
DpnII GATC 9 cut(s) 229, 253, 458, 499, 507, 645, 844, 926, 1173
DraI TTTAAA 1 cut(s) 964
DrdI GACNNNNNNGTC 2 cut(s) 414, 1132
DseDI GACNNNNNNGTC 2 cut(s) 414, 1132
EaeI YGGCCR 1 cut(s) 651
Eam1104I CTCTTC 5 cut(s) 14, 461, 534, 605, 706
EarI CTCTTC 5 cut(s) 14, 461, 534, 605, 706
Eco130I CCWWGG 3 cut(s) 182, 503, 1201
Eco24I GRGCYC 1 cut(s) 323
Eco31I GGTCTC 1 cut(s) 364
Eco47I GGWCC 3 cut(s) 212, 416, 1125
Eco57I CTGAAG 2 cut(s) 38, 399
EcoRII CCWGG 2 cut(s) 604, 1121
EcoT14I CCWWGG 3 cut(s) 182, 503, 1201
EcoT38I GRGCYC 1 cut(s) 323
ErhI CCWWGG 3 cut(s) 182, 503, 1201
FaeI CATG 6 cut(s) 186, 236, 644, 652, 743, 1169
FaqI GGGAC 3 cut(s) 138, 225, 420
FatI CATG 6 cut(s) 182, 232, 640, 648, 739, 1165
FbaI TGATCA 1 cut(s) 645
Fnu4HI GCNGC 4 cut(s) 1032, 1035, 1038, 1041
FokI GGATG 4 cut(s) 148, 885, 1115, 1169
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 4 cut(s) 1032, 1035, 1038, 1041
FspBI CTAG 1 cut(s) 497
GluI GCNGC 4 cut(s) 1032, 1035, 1038, 1041
GsaI CCCAGC 1 cut(s) 169
GsuI CTGGAG 1 cut(s) 1162
HaeIII GGCC 5 cut(s) 145, 265, 363, 653, 1012
Hin1II CATG 6 cut(s) 186, 236, 644, 652, 743, 1169
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 6 cut(s) 283, 383, 564, 782, 1079, 1106
HphI GGTGA 5 cut(s) 31, 720, 880, 902, 1105
Hpy188I TCNGA 4 cut(s) 18, 258, 1078, 1090
Hpy188III TCNNGA 2 cut(s) 685, 1166
HpyAV CCTTC 2 cut(s) 603, 777
HpyCH4V TGCA 7 cut(s) 131, 474, 593, 840, 999, 1040, 1162
HpyF10VI GCNNNNNNNGC 3 cut(s) 590, 1037, 1040
HpyF3I CTNAG 3 cut(s) 345, 441, 822
Hsp92II CATG 6 cut(s) 186, 236, 644, 652, 743, 1169
Ksp22I TGATCA 1 cut(s) 645
Kzo9I GATC 9 cut(s) 229, 253, 458, 499, 507, 645, 844, 926, 1173
LguI GCTCTTC 1 cut(s) 605
LmnI GCTCC 3 cut(s) 318, 1002, 1028
Lsp1109I GCAGC 4 cut(s) 1024, 1043, 1046, 1052
LweI GCATC 3 cut(s) 1078, 1171, 1191
MaeI CTAG 1 cut(s) 497
MaeIII GTNAC 1 cut(s) 1111
MalI GATC 9 cut(s) 231, 255, 460, 501, 509, 647, 846, 928, 1175
MboI GATC 9 cut(s) 229, 253, 458, 499, 507, 645, 844, 926, 1173
MflI RGATCY 2 cut(s) 499, 1173
MhlI GDGCHC 2 cut(s) 323, 1007
MlsI TGGCCA 1 cut(s) 653
MluCI AATT 5 cut(s) 277, 336, 751, 1060, 1084
MluNI TGGCCA 1 cut(s) 653
MlyI GAGTC 3 cut(s) 377, 558, 1115
MmeI TCCRAC 1 cut(s) 271
Mox20I TGGCCA 1 cut(s) 653
MroXI GAANNNNTTC 1 cut(s) 992
MscI TGGCCA 1 cut(s) 653
MseI TTAA 2 cut(s) 963, 1219
MslI CAYNNNNRTG 1 cut(s) 639
Msp20I TGGCCA 1 cut(s) 653
MspA1I CMGCKG 2 cut(s) 377, 1037
MspR9I CCNGG 2 cut(s) 606, 1123
Mva1269I GAATGC 1 cut(s) 521
MvaI CCWGG 2 cut(s) 606, 1123
MwoI GCNNNNNNNGC 3 cut(s) 590, 1037, 1040
NcoI CCATGG 1 cut(s) 182
NdeII GATC 9 cut(s) 229, 253, 458, 499, 507, 645, 844, 926, 1173
NlaIII CATG 6 cut(s) 186, 236, 644, 652, 743, 1169
NlaIV GGNNCC 2 cut(s) 213, 320
NmuCI GTSAC 1 cut(s) 1111
NspI RCATGY 1 cut(s) 644
PagI TCATGA 1 cut(s) 1165
PciSI GCTCTTC 1 cut(s) 605
PctI GAATGC 1 cut(s) 521
PdmI GAANNNNTTC 1 cut(s) 992
PfeI GAWTC 3 cut(s) 283, 782, 1079
PkrI GCNGC 4 cut(s) 1033, 1036, 1039, 1042
PleI GAGTC 3 cut(s) 377, 558, 1114
PpsI GAGTC 3 cut(s) 377, 558, 1114
Psp6I CCWGG 2 cut(s) 604, 1121
PspFI CCCAGC 1 cut(s) 165
PspGI CCWGG 2 cut(s) 604, 1121
PspN4I GGNNCC 2 cut(s) 213, 320
PspPI GGNCC 6 cut(s) 143, 212, 264, 416, 1010, 1125
PstI CTGCAG 2 cut(s) 1001, 1042
PstNI CAGNNNCTG 1 cut(s) 1037
PsuI RGATCY 2 cut(s) 499, 1173
PvuII CAGCTG 2 cut(s) 377, 1037
RsaI GTAC 2 cut(s) 778, 802
RsaNI GTAC 2 cut(s) 777, 801
RseI CAYNNNNRTG 1 cut(s) 639
SapI GCTCTTC 1 cut(s) 605
SaqAI TTAA 2 cut(s) 963, 1219
SatI GCNGC 4 cut(s) 1032, 1035, 1038, 1041
Sau3AI GATC 9 cut(s) 229, 253, 458, 499, 507, 645, 844, 926, 1173
Sau96I GGNCC 6 cut(s) 143, 212, 264, 416, 1010, 1125
ScaI AGTACT 1 cut(s) 778
SchI GAGTC 3 cut(s) 377, 558, 1115
ScrFI CCNGG 2 cut(s) 606, 1123
SduI GDGCHC 2 cut(s) 323, 1007
SfaNI GCATC 3 cut(s) 1078, 1171, 1191
SfcI CTRYAG 2 cut(s) 997, 1038
SinI GGWCC 3 cut(s) 212, 416, 1125
SmiMI CAYNNNNRTG 1 cut(s) 639
SmlI CTYRAG 1 cut(s) 192
SmoI CTYRAG 1 cut(s) 192
Sse9I AATT 5 cut(s) 277, 336, 751, 1060, 1084
SspMI CTAG 1 cut(s) 497
StyD4I CCNGG 2 cut(s) 604, 1121
StyI CCWWGG 3 cut(s) 182, 503, 1201
TasI AATT 5 cut(s) 277, 336, 751, 1060, 1084
TatI WGTACW 1 cut(s) 776
TfiI GAWTC 3 cut(s) 283, 782, 1079
Tru1I TTAA 2 cut(s) 963, 1219
Tru9I TTAA 2 cut(s) 963, 1219
TscAI CASTG 4 cut(s) 532, 847, 1012, 1020
TseFI GTSAC 1 cut(s) 1111
TseI GCWGC 4 cut(s) 1031, 1034, 1037, 1040
Tsp45I GTSAC 1 cut(s) 1111
TspDTI ATGAA 3 cut(s) 324, 896, 1180
TspRI CASTG 4 cut(s) 532, 847, 1012, 1020
VpaK11BI GGWCC 3 cut(s) 212, 416, 1125
XapI RAATTY 2 cut(s) 277, 1084
XceI RCATGY 1 cut(s) 644
XcmI CCANNNNNNNNNTGG 2 cut(s) 566, 920
XmnI GAANNNNTTC 1 cut(s) 992
XspI CTAG 1 cut(s) 497
ZrmI AGTACT 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.