MD12G1013600.v1.1

Tetratricopeptide repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002388
Physical Location & Seq
Reverse (-)
1425005 .. 1428056
3052 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1013600.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGCCACCGTCCTCCGAAATCCACCTTTACGAGCACCGTTCGCACCGCTTCCCGCCACCGTTTCGCCGAACCCTCCGAAAACTTCATACGTTTCGTGCAGACAAGGCTCGATCAGCACGCCGCCGTTTCTCACTCTCGTCTCTCTACGGTCATCTCGCTCGCCGTCCGTACGGTTCCTCAATTTCGCGCCTTTAGCTTCCCGCCATGAGACCCTAAGTGCGGAGACGGCGGAGGAGCTTCGAGAGCTCAATGAGGTCGAGATACTCGGTGGAGAACTAGCAGTGGAAACTCCTGCCTCAACAGTTTTGGAGAAAATTTGTGGGATCCTTTTGGTTACCAAGGATATTAAATTCATGAAGTGGCCATTTGCATGGGTATCTGCATTCTTAGTTGGACAAAGCTTATGGCTGATTTCTACTCCAATGGCACAATCACGTGATATTATCAAACCAAACGCGATTTATGAGACAGCGGAGTTATTTGAATTAGGAATCCAGCTATCATACTTGCTACTATTGCTAGGTTTTCTTGGGGTTGGAACTTTCTTTGTGATTCGTCAAGTGCTTGTACGTAGAGAACTTGATCTTTCTGCCAAAGAATTGCAGGAGCAAGTAAGAAGCGGCGATGCTGATGCGACTGAGCTTTTTGAACTTGGAGCAGTAATGCTGAGGAGGAAAATTTATCCAGCTGCTATTAAGTACTTGGTTCAGGCAATTGAGAAATGGGATGGGGACGACCAAGATCTTGCTCAGGTTTACAATGCCCTTGGTGTCAGTTACGTCCGTGAGGGTAAACTTGCTAAGGGAATTACGCAGTTTGAAACTGCCGTGAAGCTTCAGCCAGGCTACGTCACAGCTTGGAACAACCTAGGTGATGCCTATGAAAAGAGGAAAGACTTTAAGGCTGCACTGAAGGCATTTGAGGAAGTACTTCTGTTTGATCCAAATAACAAGGTAGCAATACCACGGCGGGAAACTTTGATGGAACAAGTGAAAATGTACAGAGATGTTCCGGTAAAAACAAAGGAGAAGTCATCTGACAGTATGCGTTGA

Protein Analysis

351

Amino Acids

39.17

Weight (kDa)

8.34

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 248 - 311 2e-07 Tetratricopeptide repeat
TPR_16 PF13432 255 - 314 1.2e-09 Tetratricopeptide repeat
TPR_11 PF13414 260 - 296 2.6e-06 TPR repeat
TPR_2 PF07719 285 - 317 6.9e-08 Tetratricopeptide repeat
TPR_1 PF00515 286 - 317 6.9e-09 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 188, 458
AciI CCGC 9 cut(s) 47, 54, 122, 202, 221, 230, 473, 621, 970
AclWI GGATC 3 cut(s) 319, 332, 935
AcoI YGGCCR 2 cut(s) 3, 362
AcsI RAATTY 3 cut(s) 315, 350, 678
AcuI CTGAAG 2 cut(s) 821, 932
AcvI CACGTG 1 cut(s) 437
AfaI GTAC 5 cut(s) 171, 570, 701, 930, 1001
AfiI CCNNNNNNNGG 1 cut(s) 220
AgsI TTSAA 3 cut(s) 485, 650, 821
AjnI CCWGG 1 cut(s) 841
AluBI AGCT 9 cut(s) 197, 238, 247, 402, 499, 643, 689, 835, 857
AluI AGCT 9 cut(s) 197, 238, 247, 402, 499, 643, 689, 835, 857
Alw21I GWGCWC 2 cut(s) 37, 249
Alw26I GTCTC 4 cut(s) 145, 203, 218, 461
AlwI GGATC 3 cut(s) 319, 332, 935
AoxI GGCC 2 cut(s) 3, 362
ApeKI GCWGC 2 cut(s) 689, 905
ApoI RAATTY 3 cut(s) 315, 350, 678
ArsI GACNNNNNNTTYG 2 cut(s) 1016, 1048
Asp700I GAANNNNTTC 1 cut(s) 930
AspA2I CCTAGG 1 cut(s) 868
AspLEI GCGC 1 cut(s) 190
AsuHPI GGTGA 1 cut(s) 884
AvrII CCTAGG 1 cut(s) 868
BalI TGGCCA 2 cut(s) 5, 364
BamHI GGATCC 1 cut(s) 324
BanII GRGCYC 1 cut(s) 249
BbrPI CACGTG 1 cut(s) 437
Bbv12I GWGCWC 2 cut(s) 37, 249
BbvCI CCTCAGC 1 cut(s) 668
BbvI GCAGC 2 cut(s) 676, 892
BccI CCATC 2 cut(s) 722, 976
BceAI ACGGC 5 cut(s) 109, 148, 243, 812, 983
BcgI CGANNNNNNTGC 2 cut(s) 614, 648
BciT130I CCWGG 1 cut(s) 843
BcoDI GTCTC 4 cut(s) 145, 203, 218, 461
BfaI CTAG 3 cut(s) 278, 521, 869
BglII AGATCT 1 cut(s) 742
BisI GCNGC 4 cut(s) 122, 622, 690, 906
BlnI CCTAGG 1 cut(s) 868
BlsI GCNGC 4 cut(s) 123, 623, 691, 907
BmcAI AGTACT 2 cut(s) 701, 930
Bme1390I CCNGG 1 cut(s) 843
BmiI GGNNCC 2 cut(s) 176, 326
BmrFI CCNGG 1 cut(s) 843
BmsI GCATC 3 cut(s) 616, 622, 865
Bpu10I CCTNAGC 3 cut(s) 668, 750, 801
BsaAI YACGTR 2 cut(s) 437, 572
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 4 cut(s) 339, 766, 868, 965
BsaWI WCCGGW 1 cut(s) 1012
Bsc4I CCNNNNNNNGG 1 cut(s) 220
BseBI CCWGG 1 cut(s) 843
BseDI CCNNGG 4 cut(s) 339, 766, 868, 965
BseGI GGATG 1 cut(s) 733
BseLI CCNNNNNNNGG 1 cut(s) 220
BseMII CTCAG 3 cut(s) 630, 659, 764
BseRI GAGGAG 2 cut(s) 248, 685
BseXI GCAGC 2 cut(s) 676, 892
BsgI GTGCAG 2 cut(s) 118, 891
Bsh1236I CGCG 2 cut(s) 188, 458
BshFI GGCC 2 cut(s) 5, 364
BsiHKAI GWGCWC 2 cut(s) 37, 249
BsiSI CCGG 1 cut(s) 1013
BsiWI CGTACG 1 cut(s) 169
BslFI GGGAC 1 cut(s) 746
BslI CCNNNNNNNGG 1 cut(s) 220
BsmAI GTCTC 4 cut(s) 145, 203, 218, 461
BsmBI CGTCTC 2 cut(s) 145, 218
BsmFI GGGAC 1 cut(s) 746
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 2 cut(s) 5, 364
Bso31I GGTCTC 1 cut(s) 203
Bsp1286I GDGCHC 2 cut(s) 37, 249
Bsp1407I TGTACA 1 cut(s) 999
Bsp143I GATC 5 cut(s) 111, 324, 583, 742, 940
BspACI CCGC 9 cut(s) 47, 54, 122, 202, 221, 230, 473, 621, 970
BspANI GGCC 2 cut(s) 5, 364
BspCNI CTCAG 3 cut(s) 631, 660, 763
BspFNI CGCG 2 cut(s) 188, 458
BspHI TCATGA 1 cut(s) 354
BspLI GGNNCC 2 cut(s) 176, 326
BspPI GGATC 3 cut(s) 319, 332, 935
BspTNI GGTCTC 1 cut(s) 203
BsrGI TGTACA 1 cut(s) 999
BssECI CCNNGG 4 cut(s) 339, 766, 868, 965
BssMI GATC 5 cut(s) 111, 324, 583, 742, 940
BssT1I CCWWGG 3 cut(s) 339, 766, 868
Bst2UI CCWGG 1 cut(s) 843
Bst4CI ACNGT 7 cut(s) 10, 39, 61, 150, 174, 304, 1043
BstAUI TGTACA 1 cut(s) 999
BstBAI YACGTR 2 cut(s) 437, 572
BstC8I GCNNGC 2 cut(s) 119, 161
BstDEI CTNAG 6 cut(s) 215, 388, 639, 668, 750, 801
BstDSI CCRYGG 1 cut(s) 965
BstEII GGTNACC 1 cut(s) 334
BstF5I GGATG 1 cut(s) 733
BstFNI CGCG 2 cut(s) 188, 458
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 5 cut(s) 114, 327, 586, 745, 943
BstMAI GTCTC 4 cut(s) 145, 203, 218, 461
BstMBI GATC 5 cut(s) 111, 324, 583, 742, 940
BstMWI GCNNNNNNNGC 8 cut(s) 41, 105, 114, 194, 227, 244, 517, 914
BstNI CCWGG 1 cut(s) 843
BstPI GGTNACC 1 cut(s) 334
BstSCI CCNGG 1 cut(s) 841
BstSNI TACGTA 1 cut(s) 572
BstUI CGCG 2 cut(s) 188, 458
BstV1I GCAGC 2 cut(s) 676, 892
BstX2I RGATCY 2 cut(s) 324, 742
BstXI CCANNNNNNTGG 1 cut(s) 372
BstYI RGATCY 2 cut(s) 324, 742
BsuRI GGCC 2 cut(s) 5, 364
BtgI CCRYGG 1 cut(s) 965
BtgZI GCGATG 1 cut(s) 639
BtsCI GGATG 1 cut(s) 733
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 2 cut(s) 288, 908
Cac8I GCNNGC 2 cut(s) 119, 161
CciI TCATGA 1 cut(s) 354
CfoI GCGC 1 cut(s) 190
Csp6I GTAC 5 cut(s) 170, 569, 700, 929, 1000
CviAII CATG 3 cut(s) 206, 355, 372
CviQI GTAC 5 cut(s) 170, 569, 700, 929, 1000
DdeI CTNAG 6 cut(s) 215, 388, 639, 668, 750, 801
DpnI GATC 5 cut(s) 113, 326, 585, 744, 942
DpnII GATC 5 cut(s) 111, 324, 583, 742, 940
EaeI YGGCCR 2 cut(s) 3, 362
EciI GGCGGA 1 cut(s) 245
Ecl136II GAGCTC 1 cut(s) 247
Eco105I TACGTA 1 cut(s) 572
Eco130I CCWWGG 3 cut(s) 339, 766, 868
Eco24I GRGCYC 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 203
Eco53kI GAGCTC 1 cut(s) 247
Eco57I CTGAAG 2 cut(s) 821, 932
Eco72I CACGTG 1 cut(s) 437
Eco91I GGTNACC 1 cut(s) 334
EcoICRI GAGCTC 1 cut(s) 247
EcoO65I GGTNACC 1 cut(s) 334
EcoRII CCWGG 1 cut(s) 841
EcoT14I CCWWGG 3 cut(s) 339, 766, 868
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 3 cut(s) 339, 766, 868
Esp3I CGTCTC 2 cut(s) 145, 218
FaeI CATG 3 cut(s) 209, 358, 375
FaiI YATR 9 cut(s) 88, 207, 356, 373, 406, 465, 505, 882, 1046
FaqI GGGAC 1 cut(s) 746
FatI CATG 3 cut(s) 205, 354, 371
FauI CCCGC 3 cut(s) 61, 209, 963
Fnu4HI GCNGC 4 cut(s) 122, 622, 690, 906
FokI GGATG 1 cut(s) 740
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 4 cut(s) 122, 622, 690, 906
FspBI CTAG 3 cut(s) 278, 521, 869
GlaI GCGC 1 cut(s) 189
GluI GCNGC 4 cut(s) 122, 622, 690, 906
HaeIII GGCC 2 cut(s) 5, 364
HapII CCGG 1 cut(s) 1013
HhaI GCGC 1 cut(s) 190
Hin1II CATG 3 cut(s) 209, 358, 375
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HindIII AAGCTT 2 cut(s) 400, 833
HinfI GANTC 2 cut(s) 492, 553
HpaII CCGG 1 cut(s) 1013
HphI GGTGA 1 cut(s) 884
Hpy166II GTNNAC 2 cut(s) 757, 794
Hpy188I TCNGA 3 cut(s) 17, 78, 1039
Hpy188III TCNNGA 3 cut(s) 242, 259, 355
Hpy8I GTNNAC 2 cut(s) 757, 794
HpyAV CCTTC 1 cut(s) 907
HpyCH4III ACNGT 7 cut(s) 10, 39, 61, 150, 174, 304, 1043
HpyCH4IV ACGT 5 cut(s) 90, 436, 571, 780, 849
HpyCH4V TGCA 5 cut(s) 99, 371, 383, 604, 908
HpyF10VI GCNNNNNNNGC 8 cut(s) 41, 105, 114, 194, 227, 244, 517, 914
HpyF3I CTNAG 6 cut(s) 215, 388, 639, 668, 750, 801
HpySE526I ACGT 5 cut(s) 90, 436, 571, 780, 849
Hsp92II CATG 3 cut(s) 209, 358, 375
HspAI GCGC 1 cut(s) 188
Kzo9I GATC 5 cut(s) 111, 324, 583, 742, 940
LmnI GCTCC 3 cut(s) 235, 607, 656
LpnPI CCDG 9 cut(s) 306, 509, 590, 695, 699, 737, 828, 855, 1026
Lsp1109I GCAGC 2 cut(s) 676, 892
LweI GCATC 3 cut(s) 616, 622, 865
MaeI CTAG 3 cut(s) 278, 521, 869
MaeII ACGT 5 cut(s) 90, 436, 571, 780, 849
MaeIII GTNAC 3 cut(s) 334, 776, 850
MalI GATC 5 cut(s) 113, 326, 585, 744, 942
MboI GATC 5 cut(s) 111, 324, 583, 742, 940
MfeI CAATTG 1 cut(s) 714
MflI RGATCY 2 cut(s) 324, 742
MhlI GDGCHC 2 cut(s) 37, 249
MlsI TGGCCA 2 cut(s) 5, 364
MluCI AATT 8 cut(s) 181, 315, 350, 485, 599, 678, 714, 807
MluNI TGGCCA 2 cut(s) 5, 364
MmeI TCCRAC 2 cut(s) 373, 517
Mox20I TGGCCA 2 cut(s) 5, 364
MroXI GAANNNNTTC 1 cut(s) 930
MscI TGGCCA 2 cut(s) 5, 364
MseI TTAA 3 cut(s) 348, 696, 900
MslI CAYNNNNRTG 1 cut(s) 370
Msp20I TGGCCA 2 cut(s) 5, 364
MspA1I CMGCKG 2 cut(s) 473, 689
MspI CCGG 1 cut(s) 1013
MspR9I CCNGG 1 cut(s) 843
MunI CAATTG 1 cut(s) 714
Mva1269I GAATGC 1 cut(s) 383
MvaI CCWGG 1 cut(s) 843
MvnI CGCG 2 cut(s) 188, 458
MwoI GCNNNNNNNGC 8 cut(s) 41, 105, 114, 194, 227, 244, 517, 914
NdeII GATC 5 cut(s) 111, 324, 583, 742, 940
NlaIII CATG 3 cut(s) 209, 358, 375
NlaIV GGNNCC 2 cut(s) 176, 326
NmuCI GTSAC 1 cut(s) 850
PagI TCATGA 1 cut(s) 354
PctI GAATGC 1 cut(s) 383
PdmI GAANNNNTTC 1 cut(s) 930
PfeI GAWTC 2 cut(s) 492, 553
Pfl23II CGTACG 1 cut(s) 169
PkrI GCNGC 4 cut(s) 123, 623, 691, 907
PmaCI CACGTG 1 cut(s) 437
PmlI CACGTG 1 cut(s) 437
Ppu21I YACGTR 2 cut(s) 437, 572
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 841
PspCI CACGTG 1 cut(s) 437
PspEI GGTNACC 1 cut(s) 334
PspGI CCWGG 1 cut(s) 841
PspLI CGTACG 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 176, 326
PsuI RGATCY 2 cut(s) 324, 742
PvuII CAGCTG 1 cut(s) 689
RsaI GTAC 5 cut(s) 171, 570, 701, 930, 1001
RsaNI GTAC 5 cut(s) 170, 569, 700, 929, 1000
RseI CAYNNNNRTG 1 cut(s) 370
SacI GAGCTC 1 cut(s) 249
SaqAI TTAA 3 cut(s) 348, 696, 900
SatI GCNGC 4 cut(s) 122, 622, 690, 906
Sau3AI GATC 5 cut(s) 111, 324, 583, 742, 940
ScaI AGTACT 2 cut(s) 701, 930
ScrFI CCNGG 1 cut(s) 843
SduI GDGCHC 2 cut(s) 37, 249
SfaNI GCATC 3 cut(s) 616, 622, 865
SmiMI CAYNNNNRTG 1 cut(s) 370
SnaBI TACGTA 1 cut(s) 572
Sse9I AATT 8 cut(s) 181, 315, 350, 485, 599, 678, 714, 807
SsiI CCGC 9 cut(s) 47, 54, 122, 202, 221, 230, 473, 621, 970
SspMI CTAG 3 cut(s) 278, 521, 869
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 1 cut(s) 841
StyI CCWWGG 3 cut(s) 339, 766, 868
TaaI ACNGT 7 cut(s) 10, 39, 61, 150, 174, 304, 1043
TaiI ACGT 5 cut(s) 93, 439, 574, 783, 852
TaqI TCGA 3 cut(s) 110, 241, 258
TasI AATT 8 cut(s) 181, 315, 350, 485, 599, 678, 714, 807
TatI WGTACW 3 cut(s) 699, 928, 999
TauI GCSGC 2 cut(s) 124, 624
TfiI GAWTC 2 cut(s) 492, 553
Tru1I TTAA 3 cut(s) 348, 696, 900
Tru9I TTAA 3 cut(s) 348, 696, 900
TscAI CASTG 2 cut(s) 288, 915
TseFI GTSAC 1 cut(s) 850
TseI GCWGC 2 cut(s) 689, 905
Tsp45I GTSAC 1 cut(s) 850
TspDTI ATGAA 4 cut(s) 75, 343, 371, 897
TspGWI ACGGA 2 cut(s) 157, 773
TspRI CASTG 2 cut(s) 288, 915
XapI RAATTY 3 cut(s) 315, 350, 678
XmaJI CCTAGG 1 cut(s) 868
XmnI GAANNNNTTC 1 cut(s) 930
XspI CTAG 3 cut(s) 278, 521, 869
ZrmI AGTACT 2 cut(s) 701, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.