MD14G1011000.v1.1

Tetratricopeptide repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002388
Physical Location & Seq
Reverse (-)
1064810 .. 1069187
4378 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1011000.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGCCACCTTCCTCCGAACTCCACCTTTACGAGCACCGTTCGCACCGCTTTCCTCCACCGTTTCGCCCAACCATCCGAGAACTTCATACGTCTCGTGCAGACAAGGCTCGAGCAGCACGGCGCAGTTTCTCAGTTTCAGCTCTCTTCGGTCATCTCACTCGCCGTCCGTACGGTTCCTCAAGTTCGCGCCTTTAGCTTCCCGCCATGAAACTCTAAGTGCCGAGACGGCGGAGGAGATTCGAGAGCTCAATGAGTTCGAGATACTCCGTGGAGAACTAGCAGTGGAAACTCCTGCCTCAACAATTTTGGAGAAGATTTTTGGGAACCATTTGGTTACCGGGGATGTAAAATTCATGAAGAGGCCATATGATTGGGTATCTGCATTCTTGGTTGGACAAAGCTTATTGCTGATTTCTGCTCCAATGGCTCGATCACGTGATATTATTAAACCAAATGCGGTTTATGAGACCGGGGAGTTATTTGAATTAGGAATCCAGCTATCATACTTGCTACTATTGTTAGGTTTTCTTGGGGTTGGAACTTTCTTTGTGATCCGTCAAGTGCTTGTACGTAGAGAACTTGATCTTTCTGCCAAAGAATTGCAGGAGCAAGTAAGAAGCGGTGATGCTGATGCAACTGAGCTTTTCGAACTTGGAGCAGTGATGCTGAGGAGGAAAATTTATCCTGCTGCTATTAAGTATTTGGTTCAGGCAATTGAGAAATGGGATGGGGACGACCAAGATCTTGCTCAGGTTTACAACGCCCTCGGTGTCAGTTATGTCCGTGAGGGTAAACTTGCTAAGGGAATTACGCAGTTTGAAACTGCCGTGAAGCTTCAACCGGGCTACGTCACAGCTTGGAACAACCTAGGTGATGCTTATGAAAAGAGGAAAGACTTTAAGGCTGCACTGAACGCGTTTGAAGAAGTACTGCTGTTTGATCCGAATAACAAGGTAGCAATACCACGGCGGGAAACTTTGATGCAACAAGTGAAAATGTACAGAGATGTTCCGGTGAAGACGAAGGAGAGATCATCTGACAGTATGCGTTGA

Protein Analysis

351

Amino Acids

39.35

Weight (kDa)

8.76

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_12 PF13424 248 - 311 1.6e-07 Tetratricopeptide repeat
TPR_16 PF13432 255 - 311 5.2e-09 Tetratricopeptide repeat
TPR_11 PF13414 260 - 296 2.6e-06 TPR repeat
TPR_2 PF07719 285 - 317 1.6e-07 Tetratricopeptide repeat
TPR_1 PF00515 286 - 317 2.9e-08 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 188, 917
AciI CCGC 6 cut(s) 47, 202, 230, 458, 621, 970
AclWI GGATC 2 cut(s) 547, 935
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 350, 678
AcvI CACGTG 1 cut(s) 437
AfaI GTAC 4 cut(s) 171, 570, 930, 1001
AflIII ACRYGT 1 cut(s) 915
AgsI TTSAA 4 cut(s) 485, 821, 839, 923
AluBI AGCT 8 cut(s) 141, 197, 247, 402, 499, 643, 835, 857
AluI AGCT 8 cut(s) 141, 197, 247, 402, 499, 643, 835, 857
Alw21I GWGCWC 2 cut(s) 37, 249
Alw26I GTCTC 3 cut(s) 97, 218, 461
AlwI GGATC 2 cut(s) 547, 935
Ama87I CYCGRG 1 cut(s) 109
AoxI GGCC 2 cut(s) 3, 362
ApeKI GCWGC 3 cut(s) 114, 689, 905
ApoI RAATTY 2 cut(s) 350, 678
AspA2I CCTAGG 1 cut(s) 868
AspLEI GCGC 2 cut(s) 124, 190
AsuC2I CCSGG 3 cut(s) 340, 472, 843
AsuHPI GGTGA 3 cut(s) 635, 884, 1027
AsuII TTCGAA 1 cut(s) 648
AvaI CYCGRG 1 cut(s) 109
AvrII CCTAGG 1 cut(s) 868
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 249
BauI CACGAG 1 cut(s) 94
BbrPI CACGTG 1 cut(s) 437
BbsI GAAGAC 1 cut(s) 1025
Bbv12I GWGCWC 2 cut(s) 37, 249
BbvCI CCTCAGC 1 cut(s) 668
BbvI GCAGC 3 cut(s) 126, 676, 892
BccI CCATC 2 cut(s) 81, 722
BceAI ACGGC 5 cut(s) 135, 148, 243, 812, 983
BcnI CCSGG 3 cut(s) 340, 472, 843
BcoDI GTCTC 3 cut(s) 97, 218, 461
BfaI CTAG 2 cut(s) 278, 869
BglI GCCNNNNNGGC 1 cut(s) 227
BglII AGATCT 1 cut(s) 742
BisI GCNGC 3 cut(s) 115, 690, 906
BlnI CCTAGG 1 cut(s) 868
BlsI GCNGC 3 cut(s) 116, 691, 907
BmcAI AGTACT 1 cut(s) 930
Bme1390I CCNGG 3 cut(s) 340, 472, 843
BmeT110I CYCGRG 1 cut(s) 109
BmiI GGNNCC 2 cut(s) 176, 326
BmrFI CCNGG 3 cut(s) 340, 472, 843
BmsI GCATC 5 cut(s) 616, 622, 654, 865, 972
BpiI GAAGAC 1 cut(s) 1025
Bpu10I CCTNAGC 3 cut(s) 668, 750, 801
Bpu14I TTCGAA 1 cut(s) 648
BpuEI CTTGAG 1 cut(s) 164
BpuMI CCSGG 3 cut(s) 340, 472, 843
BsaAI YACGTR 2 cut(s) 437, 572
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 6 cut(s) 268, 339, 471, 766, 868, 965
BsaWI WCCGGW 1 cut(s) 1012
BseDI CCNNGG 6 cut(s) 268, 339, 471, 766, 868, 965
BseGI GGATG 3 cut(s) 73, 349, 733
BseMII CTCAG 4 cut(s) 145, 630, 659, 764
BseRI GAGGAG 2 cut(s) 248, 685
BseXI GCAGC 3 cut(s) 126, 676, 892
BsgI GTGCAG 2 cut(s) 118, 891
Bsh1236I CGCG 2 cut(s) 188, 917
BshFI GGCC 2 cut(s) 5, 364
BsiHKAI GWGCWC 2 cut(s) 37, 249
BsiHKCI CYCGRG 1 cut(s) 109
BsiSI CCGG 4 cut(s) 339, 471, 842, 1013
BsiWI CGTACG 1 cut(s) 169
BslFI GGGAC 1 cut(s) 746
BsmAI GTCTC 3 cut(s) 97, 218, 461
BsmBI CGTCTC 2 cut(s) 97, 218
BsmFI GGGAC 1 cut(s) 746
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 2 cut(s) 5, 364
Bso31I GGTCTC 1 cut(s) 461
BsoBI CYCGRG 1 cut(s) 109
Bsp119I TTCGAA 1 cut(s) 648
Bsp1286I GDGCHC 2 cut(s) 37, 249
Bsp1407I TGTACA 1 cut(s) 999
Bsp143I GATC 6 cut(s) 431, 552, 583, 742, 940, 1031
BspACI CCGC 6 cut(s) 47, 202, 230, 458, 621, 970
BspANI GGCC 2 cut(s) 5, 364
BspCNI CTCAG 4 cut(s) 144, 631, 660, 763
BspFNI CGCG 2 cut(s) 188, 917
BspHI TCATGA 1 cut(s) 354
BspLI GGNNCC 2 cut(s) 176, 326
BspPI GGATC 2 cut(s) 547, 935
BspT104I TTCGAA 1 cut(s) 648
BspTNI GGTCTC 1 cut(s) 461
BsrGI TGTACA 1 cut(s) 999
BssECI CCNNGG 6 cut(s) 268, 339, 471, 766, 868, 965
BssMI GATC 6 cut(s) 431, 552, 583, 742, 940, 1031
BssSI CACGAG 1 cut(s) 94
BssT1I CCWWGG 1 cut(s) 868
Bst2BI CACGAG 1 cut(s) 94
Bst4CI ACNGT 4 cut(s) 39, 61, 174, 1043
Bst6I CTCTTC 2 cut(s) 150, 353
BstAUI TGTACA 1 cut(s) 999
BstBAI YACGTR 2 cut(s) 437, 572
BstBI TTCGAA 1 cut(s) 648
BstDEI CTNAG 6 cut(s) 131, 215, 639, 668, 750, 801
BstDSI CCRYGG 2 cut(s) 268, 965
BstEII GGTNACC 1 cut(s) 334
BstF5I GGATG 3 cut(s) 73, 349, 733
BstFNI CGCG 2 cut(s) 188, 917
BstHHI GCGC 2 cut(s) 124, 190
BstKTI GATC 6 cut(s) 434, 555, 586, 745, 943, 1034
BstMAI GTCTC 3 cut(s) 97, 218, 461
BstMBI GATC 6 cut(s) 431, 552, 583, 742, 940, 1031
BstMWI GCNNNNNNNGC 7 cut(s) 41, 105, 114, 194, 227, 425, 914
BstPI GGTNACC 1 cut(s) 334
BstSCI CCNGG 3 cut(s) 338, 470, 841
BstSNI TACGTA 1 cut(s) 572
BstUI CGCG 2 cut(s) 188, 917
BstV1I GCAGC 3 cut(s) 126, 676, 892
BstV2I GAAGAC 1 cut(s) 1025
BstX2I RGATCY 1 cut(s) 742
BstXI CCANNNNNNTGG 1 cut(s) 372
BstYI RGATCY 1 cut(s) 742
BsuRI GGCC 2 cut(s) 5, 364
BtgI CCRYGG 2 cut(s) 268, 965
BtsCI GGATG 3 cut(s) 73, 349, 733
BtsI GCAGTG 2 cut(s) 288, 666
BtsIMutI CAGTG 3 cut(s) 288, 666, 908
CciI TCATGA 1 cut(s) 354
CfoI GCGC 2 cut(s) 124, 190
Csp6I GTAC 4 cut(s) 170, 569, 929, 1000
CviAII CATG 2 cut(s) 206, 355
CviQI GTAC 4 cut(s) 170, 569, 929, 1000
DdeI CTNAG 6 cut(s) 131, 215, 639, 668, 750, 801
DpnI GATC 6 cut(s) 433, 554, 585, 744, 942, 1033
DpnII GATC 6 cut(s) 431, 552, 583, 742, 940, 1031
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 150, 353
EarI CTCTTC 2 cut(s) 150, 353
EciI GGCGGA 1 cut(s) 245
Ecl136II GAGCTC 1 cut(s) 247
Eco105I TACGTA 1 cut(s) 572
Eco130I CCWWGG 1 cut(s) 868
Eco24I GRGCYC 1 cut(s) 249
Eco31I GGTCTC 1 cut(s) 461
Eco53kI GAGCTC 1 cut(s) 247
Eco72I CACGTG 1 cut(s) 437
Eco88I CYCGRG 1 cut(s) 109
Eco91I GGTNACC 1 cut(s) 334
EcoICRI GAGCTC 1 cut(s) 247
EcoO65I GGTNACC 1 cut(s) 334
EcoT14I CCWWGG 1 cut(s) 868
EcoT38I GRGCYC 1 cut(s) 249
ErhI CCWWGG 1 cut(s) 868
Esp3I CGTCTC 2 cut(s) 97, 218
FaeI CATG 2 cut(s) 209, 358
FaqI GGGAC 1 cut(s) 746
FatI CATG 2 cut(s) 205, 354
FauI CCCGC 2 cut(s) 209, 963
FauNDI CATATG 1 cut(s) 367
Fnu4HI GCNGC 3 cut(s) 115, 690, 906
FokI GGATG 3 cut(s) 60, 356, 740
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 3 cut(s) 115, 690, 906
FspBI CTAG 2 cut(s) 278, 869
GlaI GCGC 2 cut(s) 123, 189
GluI GCNGC 3 cut(s) 115, 690, 906
HaeIII GGCC 2 cut(s) 5, 364
HapII CCGG 4 cut(s) 339, 471, 842, 1013
HhaI GCGC 2 cut(s) 124, 190
Hin1II CATG 2 cut(s) 209, 358
Hin6I GCGC 2 cut(s) 122, 188
HinP1I GCGC 2 cut(s) 122, 188
HindIII AAGCTT 2 cut(s) 400, 833
HinfI GANTC 2 cut(s) 238, 492
HpaII CCGG 4 cut(s) 339, 471, 842, 1013
HphI GGTGA 3 cut(s) 635, 884, 1027
Hpy166II GTNNAC 2 cut(s) 757, 794
Hpy188I TCNGA 4 cut(s) 17, 78, 945, 1039
Hpy188III TCNNGA 3 cut(s) 242, 259, 355
Hpy8I GTNNAC 2 cut(s) 757, 794
HpyAV CCTTC 2 cut(s) 19, 1018
HpyCH4III ACNGT 4 cut(s) 39, 61, 174, 1043
HpyCH4IV ACGT 4 cut(s) 90, 436, 571, 849
HpyCH4V TGCA 6 cut(s) 99, 383, 604, 635, 908, 985
HpyF10VI GCNNNNNNNGC 7 cut(s) 41, 105, 114, 194, 227, 425, 914
HpyF3I CTNAG 6 cut(s) 131, 215, 639, 668, 750, 801
HpySE526I ACGT 4 cut(s) 90, 436, 571, 849
Hsp92II CATG 2 cut(s) 209, 358
HspAI GCGC 2 cut(s) 122, 188
Kzo9I GATC 6 cut(s) 431, 552, 583, 742, 940, 1031
LmnI GCTCC 3 cut(s) 424, 607, 656
Lsp1109I GCAGC 3 cut(s) 126, 676, 892
LweI GCATC 5 cut(s) 616, 622, 654, 865, 972
MaeI CTAG 2 cut(s) 278, 869
MaeII ACGT 4 cut(s) 90, 436, 571, 849
MaeIII GTNAC 2 cut(s) 334, 850
MalI GATC 6 cut(s) 433, 554, 585, 744, 942, 1033
MboI GATC 6 cut(s) 431, 552, 583, 742, 940, 1031
MboII GAAGA 5 cut(s) 137, 325, 370, 935, 1030
MfeI CAATTG 1 cut(s) 714
MflI RGATCY 1 cut(s) 742
MhlI GDGCHC 2 cut(s) 37, 249
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 303, 350, 485, 599, 678, 714, 807
MluI ACGCGT 1 cut(s) 915
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 373, 517
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 447, 696, 900
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 339, 471, 842, 1013
MspR9I CCNGG 3 cut(s) 340, 472, 843
MunI CAATTG 1 cut(s) 714
Mva1269I GAATGC 1 cut(s) 383
MvnI CGCG 2 cut(s) 188, 917
MwoI GCNNNNNNNGC 7 cut(s) 41, 105, 114, 194, 227, 425, 914
NciI CCSGG 3 cut(s) 340, 472, 843
NdeI CATATG 1 cut(s) 367
NdeII GATC 6 cut(s) 431, 552, 583, 742, 940, 1031
NlaIII CATG 2 cut(s) 209, 358
NlaIV GGNNCC 2 cut(s) 176, 326
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 1 cut(s) 850
NspV TTCGAA 1 cut(s) 648
PaeR7I CTCGAG 1 cut(s) 109
PagI TCATGA 1 cut(s) 354
PctI GAATGC 1 cut(s) 383
PfeI GAWTC 2 cut(s) 238, 492
Pfl23II CGTACG 1 cut(s) 169
PkrI GCNGC 3 cut(s) 116, 691, 907
PmaCI CACGTG 1 cut(s) 437
PmlI CACGTG 1 cut(s) 437
Ppu21I YACGTR 2 cut(s) 437, 572
Psp124BI GAGCTC 1 cut(s) 249
PspCI CACGTG 1 cut(s) 437
PspEI GGTNACC 1 cut(s) 334
PspLI CGTACG 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 176, 326
PspXI VCTCGAGB 1 cut(s) 109
PsuI RGATCY 1 cut(s) 742
RsaI GTAC 4 cut(s) 171, 570, 930, 1001
RsaNI GTAC 4 cut(s) 170, 569, 929, 1000
SacI GAGCTC 1 cut(s) 249
SaqAI TTAA 3 cut(s) 447, 696, 900
SatI GCNGC 3 cut(s) 115, 690, 906
Sau3AI GATC 6 cut(s) 431, 552, 583, 742, 940, 1031
ScaI AGTACT 1 cut(s) 930
ScrFI CCNGG 3 cut(s) 340, 472, 843
SduI GDGCHC 2 cut(s) 37, 249
SfaNI GCATC 5 cut(s) 616, 622, 654, 865, 972
Sfr274I CTCGAG 1 cut(s) 109
SfuI TTCGAA 1 cut(s) 648
SlaI CTCGAG 1 cut(s) 109
SmlI CTYRAG 2 cut(s) 109, 179
SmoI CTYRAG 2 cut(s) 109, 179
SnaBI TACGTA 1 cut(s) 572
Sse9I AATT 7 cut(s) 303, 350, 485, 599, 678, 714, 807
SsiI CCGC 6 cut(s) 47, 202, 230, 458, 621, 970
SspMI CTAG 2 cut(s) 278, 869
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 3 cut(s) 338, 470, 841
StyI CCWWGG 1 cut(s) 868
TaaI ACNGT 4 cut(s) 39, 61, 174, 1043
TaiI ACGT 4 cut(s) 93, 439, 574, 852
TaqI TCGA 5 cut(s) 110, 241, 258, 430, 648
TaqII GACCGA 1 cut(s) 138
TasI AATT 7 cut(s) 303, 350, 485, 599, 678, 714, 807
TatI WGTACW 2 cut(s) 928, 999
TfiI GAWTC 2 cut(s) 238, 492
Tru1I TTAA 3 cut(s) 447, 696, 900
Tru9I TTAA 3 cut(s) 447, 696, 900
TscAI CASTG 3 cut(s) 288, 666, 915
TseFI GTSAC 1 cut(s) 850
TseI GCWGC 3 cut(s) 114, 689, 905
Tsp45I GTSAC 1 cut(s) 850
TspDTI ATGAA 5 cut(s) 75, 222, 343, 371, 897
TspGWI ACGGA 4 cut(s) 157, 257, 545, 773
TspRI CASTG 3 cut(s) 288, 666, 915
XapI RAATTY 2 cut(s) 350, 678
XhoI CTCGAG 1 cut(s) 109
XmaJI CCTAGG 1 cut(s) 868
XspI CTAG 2 cut(s) 278, 869
ZrmI AGTACT 1 cut(s) 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.