MD12G1034600.v1.1

UPF0554 protein C2orf43 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002532
Physical Location & Seq
Reverse (-)
3769362 .. 3775743
6382 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1034600.v1.1.491

Sequence Viewer

Length: 951 bp
ATGTGTCAAGGTTCTTACTTTTTTGGCTCCAAATTCTTTGTGGTGTGTGATAGTTTTAGTGGCGATTGTTTTGTTAGTTGGGTTTTTCTTTGTTTCAGTTTCACCACTGAAATACTGGAGATCCCTGCTGACAATCCAGCATTGCATGTTCTTGTTATACCTGGAAATCCAGGTGTTGTTTCATTTTACAAAGACTTTGTGGAGTCGCTGTATGAGTTGCTGGGTGGAACTGCTTCTGTGACAGGCAAGACTTTGGTGTTTGTTAACTGGGAGCATGGAAGGCTGTTCTCATTACAAGAACAAATTGATCATAAGATGGACTTCGTCAAACAGGAGTTGCAAAATAATGAAGTTCCTATATTACTGGTTGGGCACTCTATTGGTTCATATATATCTCTAGAAATGTTCCGGAAGTCTCCAGAGAAGGTGACATTTTGTGTTGGACTATATCCTTTTTTGGCCTTAAACCGACAATCTGTACAGCAGTCTTTCATCAGGAAGCTTGCAGCGTCCCGTATTCTAAGTGTTGTCTTGAGCTTAATTGTTGCACCCTTGGGATTGTTTCCTATCCGATTGTTGAGGTTAATTGTGACCACATTCCTTGGGAAGCCCTGGTCAGCTACTGCAGTTGAGGCTACTTGCTCTCACTTGGTAAAGTACCATACCATGCGGAATGTCCTCTTCATGGCAATGACAGAATTCAGAGAGCTTTCAGCAACACCAGATTGGGCATTCATGAGGGGAAACCAGCACAAAATAGCATTTTTGTTCGGCATTGATGATCACTGGGGTCCATTGCAAATGTTTGAAGAGATTTCCAAGCAGGTCCCGGATGTTGCTCTATCAATTGAAAGAGAGGGCCACACTCATGCTTTTGGTTGCACTGAGGCTGGCTCATTGTGGGTTGCTGAGCATGTAGCAGGCTTGATCAAGAACAATCAAGCATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIDHydrolase

Protein Analysis

317

Amino Acids

35.46

Weight (kDa)

6.12

Isoelectric Point (pI)

33.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LIDHydrolase PF10230 48 - 292 4.6e-64 Lipid-droplet associated hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 814
AccIII TCCGGA 1 cut(s) 408
AciI CCGC 1 cut(s) 670
AclWI GGATC 1 cut(s) 115
AcsI RAATTY 2 cut(s) 32, 698
AfaI GTAC 2 cut(s) 480, 659
AfiI CCNNNNNNNGG 1 cut(s) 685
AgsI TTSAA 2 cut(s) 809, 851
AjnI CCWGG 3 cut(s) 160, 169, 611
AluBI AGCT 4 cut(s) 502, 537, 620, 709
AluI AGCT 4 cut(s) 502, 537, 620, 709
Alw26I GTCTC 1 cut(s) 420
AlwI GGATC 1 cut(s) 115
AlwNI CAGNNNCTG 1 cut(s) 623
Aor13HI TCCGGA 1 cut(s) 408
AoxI GGCC 2 cut(s) 459, 859
ApeKI GCWGC 1 cut(s) 506
ApoI RAATTY 2 cut(s) 32, 698
Asp700I GAANNNNTTC 1 cut(s) 232
AspS9I GGNCC 3 cut(s) 791, 826, 859
AsuC2I CCSGG 1 cut(s) 830
AsuHPI GGTGA 2 cut(s) 94, 439
AvaII GGWCC 2 cut(s) 791, 826
BaeGI GKGCMC 1 cut(s) 375
BbvI GCAGC 1 cut(s) 518
BccI CCATC 1 cut(s) 310
BciT130I CCWGG 3 cut(s) 162, 171, 613
BclI TGATCA 3 cut(s) 307, 781, 927
BcnI CCSGG 1 cut(s) 830
BcoDI GTCTC 1 cut(s) 420
BfaI CTAG 1 cut(s) 398
BfmI CTRYAG 1 cut(s) 624
BfuAI ACCTGC 1 cut(s) 814
BisI GCNGC 1 cut(s) 507
BlpI GCTNAGC 1 cut(s) 909
BlsI GCNGC 1 cut(s) 508
Bme1390I CCNGG 4 cut(s) 162, 171, 613, 830
Bme18I GGWCC 2 cut(s) 791, 826
BmgT120I GGNCC 3 cut(s) 791, 826, 859
BmiI GGNNCC 3 cut(s) 28, 792, 828
BmrFI CCNGG 4 cut(s) 162, 171, 613, 830
BmrI ACTGGG 2 cut(s) 277, 796
BmuI ACTGGG 2 cut(s) 277, 796
BplI GAGNNNNNCTC 2 cut(s) 878, 910
BpmI CTGGAG 2 cut(s) 137, 402
Bpu1102I GCTNAGC 1 cut(s) 909
BpuEI CTTGAG 1 cut(s) 553
BpuMI CCSGG 1 cut(s) 830
BsaJI CCNNGG 3 cut(s) 552, 601, 611
BsaWI WCCGGW 1 cut(s) 408
BsaXI ACNNNNNCTCC 2 cut(s) 194, 224
Bsc4I CCNNNNNNNGG 1 cut(s) 685
Bse1I ACTGG 4 cut(s) 120, 272, 369, 791
Bse3DI GCAATG 3 cut(s) 140, 696, 794
BseAI TCCGGA 1 cut(s) 408
BseBI CCWGG 3 cut(s) 162, 171, 613
BseDI CCNNGG 3 cut(s) 552, 601, 611
BseGI GGATG 1 cut(s) 838
BseLI CCNNNNNNNGG 1 cut(s) 685
BseMI GCAATG 3 cut(s) 140, 696, 794
BseMII CTCAG 2 cut(s) 876, 900
BseNI ACTGG 4 cut(s) 120, 272, 369, 791
BseSI GKGCMC 1 cut(s) 375
BseXI GCAGC 1 cut(s) 518
BseYI CCCAGC 1 cut(s) 220
BshFI GGCC 2 cut(s) 461, 861
BsiSI CCGG 2 cut(s) 409, 830
BslFI GGGAC 2 cut(s) 496, 812
BslI CCNNNNNNNGG 1 cut(s) 685
BsmAI GTCTC 1 cut(s) 420
BsmFI GGGAC 2 cut(s) 496, 812
BsmI GAATGC 1 cut(s) 731
BsnI GGCC 2 cut(s) 461, 861
Bsp1286I GDGCHC 1 cut(s) 375
Bsp13I TCCGGA 1 cut(s) 408
Bsp1407I TGTACA 1 cut(s) 478
Bsp143I GATC 4 cut(s) 120, 307, 781, 927
Bsp1720I GCTNAGC 1 cut(s) 909
BspACI CCGC 1 cut(s) 670
BspANI GGCC 2 cut(s) 461, 861
BspCNI CTCAG 2 cut(s) 877, 901
BspEI TCCGGA 1 cut(s) 408
BspHI TCATGA 1 cut(s) 735
BspLI GGNNCC 3 cut(s) 28, 792, 828
BspMAI CTGCAG 1 cut(s) 628
BspMI ACCTGC 1 cut(s) 814
BspPI GGATC 1 cut(s) 115
BsrDI GCAATG 3 cut(s) 140, 696, 794
BsrGI TGTACA 1 cut(s) 478
BsrI ACTGG 4 cut(s) 120, 272, 369, 791
BssECI CCNNGG 3 cut(s) 552, 601, 611
BssMI GATC 4 cut(s) 120, 307, 781, 927
BssT1I CCWWGG 2 cut(s) 552, 601
Bst2UI CCWGG 3 cut(s) 162, 171, 613
Bst6I CTCTTC 2 cut(s) 686, 804
BstAUI TGTACA 1 cut(s) 478
BstC8I GCNNGC 3 cut(s) 504, 892, 922
BstDEI CTNAG 3 cut(s) 521, 885, 909
BstF5I GGATG 1 cut(s) 838
BstKTI GATC 4 cut(s) 123, 310, 784, 930
BstMAI GTCTC 1 cut(s) 420
BstMBI GATC 4 cut(s) 120, 307, 781, 927
BstMWI GCNNNNNNNGC 2 cut(s) 280, 632
BstNI CCWGG 3 cut(s) 162, 171, 613
BstNSI RCATGY 2 cut(s) 149, 917
BstSCI CCNGG 4 cut(s) 160, 169, 611, 828
BstSFI CTRYAG 1 cut(s) 624
BstSLI GKGCMC 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 518
BstX2I RGATCY 1 cut(s) 120
BstYI RGATCY 1 cut(s) 120
BsuRI GGCC 2 cut(s) 461, 861
BtsCI GGATG 1 cut(s) 838
BtsIMutI CAGTG 3 cut(s) 105, 784, 882
BveI ACCTGC 1 cut(s) 814
Cac8I GCNNGC 3 cut(s) 504, 892, 922
CaiI CAGNNNCTG 1 cut(s) 623
CciI TCATGA 1 cut(s) 735
Cfr13I GGNCC 3 cut(s) 791, 826, 859
CseI GACGC 1 cut(s) 498
Csp6I GTAC 2 cut(s) 479, 658
CspCI CAANNNNNGTGG 2 cut(s) 583, 618
CviAII CATG 7 cut(s) 146, 275, 667, 685, 736, 869, 914
CviQI GTAC 2 cut(s) 479, 658
DdeI CTNAG 3 cut(s) 521, 885, 909
DpnI GATC 4 cut(s) 122, 309, 783, 929
DpnII GATC 4 cut(s) 120, 307, 781, 927
Eam1104I CTCTTC 2 cut(s) 686, 804
EarI CTCTTC 2 cut(s) 686, 804
Eco130I CCWWGG 2 cut(s) 552, 601
Eco47I GGWCC 2 cut(s) 791, 826
EcoO109I RGGNCCY 1 cut(s) 826
EcoRI GAATTC 1 cut(s) 698
EcoRII CCWGG 3 cut(s) 160, 169, 611
EcoT14I CCWWGG 2 cut(s) 552, 601
ErhI CCWWGG 2 cut(s) 552, 601
FaeI CATG 7 cut(s) 149, 278, 670, 688, 739, 872, 917
FalI AAGNNNNNCTT 2 cut(s) 305, 337
FaqI GGGAC 2 cut(s) 496, 812
FatI CATG 7 cut(s) 145, 274, 666, 684, 735, 868, 913
FbaI TGATCA 3 cut(s) 307, 781, 927
Fnu4HI GCNGC 1 cut(s) 507
FokI GGATG 1 cut(s) 845
Fsp4HI GCNGC 1 cut(s) 507
FspBI CTAG 1 cut(s) 398
GluI GCNGC 1 cut(s) 507
GsaI CCCAGC 1 cut(s) 224
GsuI CTGGAG 2 cut(s) 137, 402
HaeIII GGCC 2 cut(s) 461, 861
HapII CCGG 2 cut(s) 409, 830
HgaI GACGC 1 cut(s) 498
Hin1II CATG 7 cut(s) 149, 278, 670, 688, 739, 872, 917
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HindIII AAGCTT 1 cut(s) 500
HinfI GANTC 1 cut(s) 203
HpaI GTTAAC 1 cut(s) 265
HpaII CCGG 2 cut(s) 409, 830
HphI GGTGA 2 cut(s) 94, 439
Hpy166II GTNNAC 1 cut(s) 265
Hpy188I TCNGA 2 cut(s) 572, 704
Hpy188III TCNNGA 7 cut(s) 398, 409, 419, 496, 532, 736, 931
Hpy8I GTNNAC 1 cut(s) 265
HpyAV CCTTC 2 cut(s) 273, 418
HpyCH4V TGCA 7 cut(s) 145, 340, 506, 548, 626, 799, 882
HpyF10VI GCNNNNNNNGC 2 cut(s) 280, 632
HpyF3I CTNAG 3 cut(s) 521, 885, 909
Hsp92II CATG 7 cut(s) 149, 278, 670, 688, 739, 872, 917
Kpn2I TCCGGA 1 cut(s) 408
Ksp22I TGATCA 3 cut(s) 307, 781, 927
KspAI GTTAAC 1 cut(s) 265
Kzo9I GATC 4 cut(s) 120, 307, 781, 927
LmnI GCTCC 2 cut(s) 32, 271
Lsp1109I GCAGC 1 cut(s) 518
MaeI CTAG 1 cut(s) 398
MaeIII GTNAC 3 cut(s) 238, 427, 589
MalI GATC 4 cut(s) 122, 309, 783, 929
MboI GATC 4 cut(s) 120, 307, 781, 927
MboII GAAGA 2 cut(s) 673, 821
MfeI CAATTG 1 cut(s) 846
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 1 cut(s) 375
MluCI AATT 6 cut(s) 32, 303, 540, 585, 698, 846
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 6 cut(s) 573, 625, 689, 732, 850, 880
MroI TCCGGA 1 cut(s) 408
MroXI GAANNNNTTC 1 cut(s) 232
MseI TTAA 4 cut(s) 264, 464, 539, 584
MslI CAYNNNNRTG 2 cut(s) 689, 867
MspI CCGG 2 cut(s) 409, 830
MspR9I CCNGG 4 cut(s) 162, 171, 613, 830
MunI CAATTG 1 cut(s) 846
Mva1269I GAATGC 1 cut(s) 731
MvaI CCWGG 3 cut(s) 162, 171, 613
MwoI GCNNNNNNNGC 2 cut(s) 280, 632
NciI CCSGG 1 cut(s) 830
NdeII GATC 4 cut(s) 120, 307, 781, 927
NlaIII CATG 7 cut(s) 149, 278, 670, 688, 739, 872, 917
NlaIV GGNNCC 3 cut(s) 28, 792, 828
NmuCI GTSAC 3 cut(s) 238, 427, 589
NspI RCATGY 2 cut(s) 149, 917
PagI TCATGA 1 cut(s) 735
PctI GAATGC 1 cut(s) 731
PdmI GAANNNNTTC 1 cut(s) 232
PflFI GACNNNGTC 1 cut(s) 323
PfoI TCCNGGA 1 cut(s) 828
PkrI GCNGC 1 cut(s) 508
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
PpuMI RGGWCCY 1 cut(s) 826
Psp5II RGGWCCY 1 cut(s) 826
Psp6I CCWGG 3 cut(s) 160, 169, 611
PspFI CCCAGC 1 cut(s) 220
PspGI CCWGG 3 cut(s) 160, 169, 611
PspN4I GGNNCC 3 cut(s) 28, 792, 828
PspPI GGNCC 3 cut(s) 791, 826, 859
PspPPI RGGWCCY 1 cut(s) 826
PstI CTGCAG 1 cut(s) 628
PstNI CAGNNNCTG 1 cut(s) 623
PsuI RGATCY 1 cut(s) 120
PsyI GACNNNGTC 1 cut(s) 323
RsaI GTAC 2 cut(s) 480, 659
RsaNI GTAC 2 cut(s) 479, 658
RseI CAYNNNNRTG 2 cut(s) 689, 867
SaqAI TTAA 4 cut(s) 264, 464, 539, 584
SatI GCNGC 1 cut(s) 507
Sau3AI GATC 4 cut(s) 120, 307, 781, 927
Sau96I GGNCC 3 cut(s) 791, 826, 859
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 4 cut(s) 162, 171, 613, 830
SduI GDGCHC 1 cut(s) 375
SfcI CTRYAG 1 cut(s) 624
SinI GGWCC 2 cut(s) 791, 826
SmiMI CAYNNNNRTG 2 cut(s) 689, 867
SmlI CTYRAG 1 cut(s) 532
SmoI CTYRAG 1 cut(s) 532
Sse9I AATT 6 cut(s) 32, 303, 540, 585, 698, 846
SsiI CCGC 1 cut(s) 670
SspMI CTAG 1 cut(s) 398
StyD4I CCNGG 4 cut(s) 160, 169, 611, 828
StyI CCWWGG 2 cut(s) 552, 601
TasI AATT 6 cut(s) 32, 303, 540, 585, 698, 846
TatI WGTACW 1 cut(s) 478
Tru1I TTAA 4 cut(s) 264, 464, 539, 584
Tru9I TTAA 4 cut(s) 264, 464, 539, 584
TscAI CASTG 3 cut(s) 112, 791, 889
TseFI GTSAC 3 cut(s) 238, 427, 589
TseI GCWGC 1 cut(s) 506
Tsp45I GTSAC 3 cut(s) 238, 427, 589
TspDTI ATGAA 6 cut(s) 171, 363, 375, 481, 673, 724
TspRI CASTG 3 cut(s) 112, 791, 889
Tth111I GACNNNGTC 1 cut(s) 323
VpaK11BI GGWCC 2 cut(s) 791, 826
XapI RAATTY 2 cut(s) 32, 698
XbaI TCTAGA 1 cut(s) 397
XceI RCATGY 2 cut(s) 149, 917
XcmI CCANNNNNNNNNTGG 2 cut(s) 37, 112
XmnI GAANNNNTTC 1 cut(s) 232
XspI CTAG 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.