Rorug03G0147900

UPF0554 protein C2orf43 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002532
Physical Location & Seq
Forward (+)
12138471 .. 12139028
558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0147900.1

Sequence Viewer

Length: 558 bp
ATGGACATAAGCCAGGTAGATGATGTTGTTCTCGCCGGTGGATCTTCTAGAATTCCCAAAGTGCAGCAATTATTGCAAGATGTCTTCAAGGGAAAAAAGCTATGCAAGAGCACTAATCCAGATGAGGCAATAGCATACGGTGCTGCTGTTCAAGCTGCAACTCTGAGTGGGAATGGAAATTGGAAGCTTCATGACTTCACTCTCTTGGATGTCACACCTATGTCACTTGGAGTGCGGACTACAAATGAAAATATCATGCATTTTGTTATTCCAAGAAACACCAGAATTCCGGTCAAGAAGAATACAACAATATGTACCCTTTATGACAACCAAAGTTCCGTTGCCTTTCCAATTTTTGAGGGTGAGAGTAAAACCACTCTGAACAATAACCTTTTGGGTAAATTTAGCCTCGTGGACATTCCTCCGGCTCCTAAGGGTTATCCATTCGACGTTTGCTTTGAAATTGATGAAAATGGTATCCTAGGTGTCTCCGCTGAGGACAAGTCCACTGGCCAGAAGAAAGGGATTACAATCACGAGTGACAGAAGAAATTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIDHydrolase

Protein Analysis

185

Amino Acids

20.15

Weight (kDa)

6.72

Isoelectric Point (pI)

27.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 2 - 182 1.9e-56 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 235, 492
AclWI GGATC 1 cut(s) 49
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 3 cut(s) 51, 285, 401
AfaI GTAC 1 cut(s) 316
AgsI TTSAA 3 cut(s) 88, 152, 461
AjnI CCWGG 1 cut(s) 12
AluBI AGCT 3 cut(s) 100, 155, 187
AluI AGCT 3 cut(s) 100, 155, 187
Alw21I GWGCWC 1 cut(s) 113
Alw26I GTCTC 1 cut(s) 493
AlwI GGATC 1 cut(s) 49
AoxI GGCC 1 cut(s) 511
ApeKI GCWGC 3 cut(s) 64, 143, 155
ApoI RAATTY 3 cut(s) 51, 285, 401
ArsI GACNNNNNNTTYG 2 cut(s) 440, 472
AspA2I CCTAGG 1 cut(s) 481
AsuHPI GGTGA 1 cut(s) 374
AvrII CCTAGG 1 cut(s) 481
AxyI CCTNAGG 1 cut(s) 432
BalI TGGCCA 1 cut(s) 513
BauI CACGAG 2 cut(s) 410, 535
BbsI GAAGAC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 113
BbvCI CCTCAGC 1 cut(s) 495
BbvI GCAGC 3 cut(s) 76, 130, 142
BciT130I CCWGG 1 cut(s) 14
BciVI GTATCC 1 cut(s) 488
BcoDI GTCTC 1 cut(s) 493
BfaI CTAG 2 cut(s) 48, 482
BfuI GTATCC 1 cut(s) 488
BisI GCNGC 3 cut(s) 65, 144, 156
BlnI CCTAGG 1 cut(s) 481
BlsI GCNGC 3 cut(s) 66, 145, 157
Bme1390I CCNGG 1 cut(s) 14
BmiI GGNNCC 1 cut(s) 429
BmrFI CCNGG 1 cut(s) 14
BpiI GAAGAC 1 cut(s) 76
Bpu10I CCTNAGC 1 cut(s) 495
BsaBI GATNNNNATC 1 cut(s) 530
BsaJI CCNNGG 1 cut(s) 481
BsaWI WCCGGW 1 cut(s) 289
Bse118I RCCGGY 1 cut(s) 35
Bse1I ACTGG 1 cut(s) 514
Bse21I CCTNAGG 1 cut(s) 432
Bse8I GATNNNNATC 1 cut(s) 530
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 1 cut(s) 481
BseGI GGATG 1 cut(s) 214
BseJI GATNNNNATC 1 cut(s) 530
BseMII CTCAG 2 cut(s) 155, 486
BseNI ACTGG 1 cut(s) 514
BseXI GCAGC 3 cut(s) 76, 130, 142
BsgI GTGCAG 1 cut(s) 83
BshFI GGCC 1 cut(s) 513
BsiHKAI GWGCWC 1 cut(s) 113
BsiSI CCGG 3 cut(s) 36, 290, 425
BsmAI GTCTC 1 cut(s) 493
BsnI GGCC 1 cut(s) 513
Bsp1286I GDGCHC 1 cut(s) 113
Bsp143I GATC 1 cut(s) 41
BspACI CCGC 2 cut(s) 235, 492
BspANI GGCC 1 cut(s) 513
BspCNI CTCAG 2 cut(s) 156, 487
BspHI TCATGA 1 cut(s) 190
BspLI GGNNCC 1 cut(s) 429
BspPI GGATC 1 cut(s) 49
BsrFI RCCGGY 1 cut(s) 35
BsrI ACTGG 1 cut(s) 514
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 1 cut(s) 481
BssMI GATC 1 cut(s) 41
BssSI CACGAG 2 cut(s) 410, 535
BssT1I CCWWGG 1 cut(s) 481
Bst2BI CACGAG 2 cut(s) 410, 535
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 1 cut(s) 140
BstAPI GCANNNNNTGC 2 cut(s) 73, 140
BstDEI CTNAG 3 cut(s) 164, 432, 495
BstF5I GGATG 1 cut(s) 214
BstKTI GATC 1 cut(s) 44
BstMAI GTCTC 1 cut(s) 493
BstMBI GATC 1 cut(s) 41
BstMWI GCNNNNNNNGC 3 cut(s) 73, 140, 152
BstNI CCWGG 1 cut(s) 14
BstSCI CCNGG 1 cut(s) 12
BstV1I GCAGC 3 cut(s) 76, 130, 142
BstV2I GAAGAC 1 cut(s) 76
BstX2I RGATCY 1 cut(s) 41
BstYI RGATCY 1 cut(s) 41
Bsu36I CCTNAGG 1 cut(s) 432
BsuI GTATCC 1 cut(s) 488
BsuRI GGCC 1 cut(s) 513
BtsCI GGATG 1 cut(s) 214
BtsIMutI CAGTG 1 cut(s) 507
CciI TCATGA 1 cut(s) 190
Cfr10I RCCGGY 1 cut(s) 35
Csp6I GTAC 1 cut(s) 315
CviAII CATG 2 cut(s) 191, 256
CviJI RGCY 7 cut(s) 12, 100, 155, 187, 408, 428, 513
CviKI_1 RGCY 7 cut(s) 12, 100, 155, 187, 408, 428, 513
CviQI GTAC 1 cut(s) 315
DdeI CTNAG 3 cut(s) 164, 432, 495
DpnI GATC 1 cut(s) 43
DpnII GATC 1 cut(s) 41
EaeI YGGCCR 1 cut(s) 511
Eco130I CCWWGG 1 cut(s) 481
Eco81I CCTNAGG 1 cut(s) 432
EcoRI GAATTC 2 cut(s) 51, 285
EcoRII CCWGG 1 cut(s) 12
EcoT14I CCWWGG 1 cut(s) 481
EcoT22I ATGCAT 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 481
FaeI CATG 2 cut(s) 194, 259
FaiI YATR 8 cut(s) 8, 103, 136, 192, 221, 257, 313, 324
FatI CATG 2 cut(s) 190, 255
Fnu4HI GCNGC 3 cut(s) 65, 144, 156
FokI GGATG 1 cut(s) 221
Fsp4HI GCNGC 3 cut(s) 65, 144, 156
FspBI CTAG 2 cut(s) 48, 482
GluI GCNGC 3 cut(s) 65, 144, 156
HaeIII GGCC 1 cut(s) 513
HapII CCGG 3 cut(s) 36, 290, 425
Hin1II CATG 2 cut(s) 194, 259
HindIII AAGCTT 1 cut(s) 185
HpaII CCGG 3 cut(s) 36, 290, 425
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 2 cut(s) 415, 507
Hpy188I TCNGA 2 cut(s) 165, 381
Hpy188III TCNNGA 5 cut(s) 48, 119, 191, 295, 535
Hpy8I GTNNAC 2 cut(s) 415, 507
Hpy99I CGWCG 1 cut(s) 452
HpyCH4III ACNGT 1 cut(s) 140
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 5 cut(s) 64, 76, 105, 158, 259
HpyF10VI GCNNNNNNNGC 3 cut(s) 73, 140, 152
HpyF3I CTNAG 3 cut(s) 164, 432, 495
HpySE526I ACGT 1 cut(s) 450
Hsp92II CATG 2 cut(s) 194, 259
Kzo9I GATC 1 cut(s) 41
LmnI GCTCC 1 cut(s) 433
LpnPI CCDG 8 cut(s) 26, 49, 132, 295, 303, 438, 495, 527
Lsp1109I GCAGC 3 cut(s) 76, 130, 142
MaeI CTAG 2 cut(s) 48, 482
MaeII ACGT 1 cut(s) 450
MaeIII GTNAC 3 cut(s) 211, 222, 539
MalI GATC 1 cut(s) 43
MboI GATC 1 cut(s) 41
MboII GAAGA 5 cut(s) 36, 76, 310, 529, 558
MflI RGATCY 1 cut(s) 41
MhlI GDGCHC 1 cut(s) 113
MlsI TGGCCA 1 cut(s) 513
MluCI AATT 8 cut(s) 51, 68, 178, 285, 351, 401, 462, 550
MluNI TGGCCA 1 cut(s) 513
MnlI CCTC 5 cut(s) 118, 352, 419, 432, 490
Mox20I TGGCCA 1 cut(s) 513
Mph1103I ATGCAT 1 cut(s) 261
MscI TGGCCA 1 cut(s) 513
MslI CAYNNNNRTG 1 cut(s) 218
Msp20I TGGCCA 1 cut(s) 513
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 3 cut(s) 36, 290, 425
MspR9I CCNGG 1 cut(s) 14
MvaI CCWGG 1 cut(s) 14
MwoI GCNNNNNNNGC 3 cut(s) 73, 140, 152
NdeII GATC 1 cut(s) 41
NlaIII CATG 2 cut(s) 194, 259
NlaIV GGNNCC 1 cut(s) 429
NmuCI GTSAC 3 cut(s) 211, 222, 539
NsiI ATGCAT 1 cut(s) 261
PagI TCATGA 1 cut(s) 190
PkrI GCNGC 3 cut(s) 66, 145, 157
Psp6I CCWGG 1 cut(s) 12
PspGI CCWGG 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 429
PsuI RGATCY 1 cut(s) 41
RsaI GTAC 1 cut(s) 316
RsaNI GTAC 1 cut(s) 315
RseI CAYNNNNRTG 1 cut(s) 218
SatI GCNGC 3 cut(s) 65, 144, 156
Sau3AI GATC 1 cut(s) 41
ScrFI CCNGG 1 cut(s) 14
SduI GDGCHC 1 cut(s) 113
SetI ASST 8 cut(s) 18, 102, 157, 189, 220, 393, 453, 487
SgrAI CRCCGGYG 1 cut(s) 35
SmiMI CAYNNNNRTG 1 cut(s) 218
Sse9I AATT 8 cut(s) 51, 68, 178, 285, 351, 401, 462, 550
SsiI CCGC 2 cut(s) 235, 492
SspMI CTAG 2 cut(s) 48, 482
StyD4I CCNGG 1 cut(s) 12
StyI CCWWGG 1 cut(s) 481
TaaI ACNGT 1 cut(s) 140
TaiI ACGT 1 cut(s) 453
TaqI TCGA 1 cut(s) 447
TasI AATT 8 cut(s) 51, 68, 178, 285, 351, 401, 462, 550
TscAI CASTG 1 cut(s) 514
TseFI GTSAC 3 cut(s) 211, 222, 539
TseI GCWGC 3 cut(s) 64, 143, 155
Tsp45I GTSAC 3 cut(s) 211, 222, 539
TspDTI ATGAA 3 cut(s) 179, 261, 483
TspGWI ACGGA 1 cut(s) 328
TspRI CASTG 1 cut(s) 514
XapI RAATTY 3 cut(s) 51, 285, 401
XbaI TCTAGA 1 cut(s) 47
XmaJI CCTAGG 1 cut(s) 481
XspI CTAG 2 cut(s) 48, 482
Zsp2I ATGCAT 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.