Rh3DG223400

UPF0554 protein C2orf43 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0002532
Physical Location & Seq
Reverse (-)
20980390 .. 20986060
5671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG223400.1

Sequence Viewer

Length: 768 bp
ATGCTTCGTGGGTTCATTTCTCCCATTACACGATCCCCTCTCACTCTCTTATGTTTCAGGCCAAGGTTCTCTGGCCTTTATTGTCGGAGCAGAGAAATGGATCAGGGCATTTCACAACGTGAAAACCAAGTGCTTCGAGTGAAGAAGCGTGTCGCATTTCGTTTATGCGATGTGTCGAGTCACACTACTGAAGTAATGGAGATTAAAGCTGACAATCCAACATTTCATGTTCTAATCATCCCTGGAAATCCAGGTGTTGTTACATTTTATAAAGACTTTGTGGAGGCTCTATATGAGCTGCTGGGTGGAACTGCGTCTGTGACAGTTGTTGGGCATATATCACACACCAAAAAGAACTGGGAGCATGGAAGGATTTTCTCATTACAAGCCCAAATTAATCATAAGATGGACTTCATCAAACAGGAATTGCAAAATACTGCAGGCCCTTTATTACTGGTAGGGCACTCTATTGGATCATATATATCCATGGAAATGTTTAGGAGGTCTCCAGAACAGGTGACTTATTGTGTTGGGCTATATCCGTTTTTGGCGTTAAACCCAGAGTCCACAAAACAGGCCCTTATTGGGAAGATTACAGAGTCCCGCATTGTATGTGTTGCCTTGAGCTTTGTTGTTGCGCTCTTGGGATTGTTGCCTATATCACTTTTGAGGTTAATTGTGCTCACATTCCTTGGGAAGTCATGGTCAGCTGCTGCAGTTGAGGCTGCTTGCTATCACTTGGTAAAGGTAAAGAAATGTGTGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIDHydrolase

Protein Analysis

255

Amino Acids

28.54

Weight (kDa)

9.38

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LIDHydrolase PF10230 74 - 249 2.1e-44 Lipid-droplet associated hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 270
AciI CCGC 1 cut(s) 604
AclWI GGATC 3 cut(s) 27, 108, 481
AcuI CTGAAG 1 cut(s) 210
AdeI CACNNNGTG 1 cut(s) 119
AfiI CCNNNNNNNGG 1 cut(s) 585
AjnI CCWGG 2 cut(s) 241, 250
AluBI AGCT 4 cut(s) 209, 298, 627, 710
AluI AGCT 4 cut(s) 209, 298, 627, 710
Alw21I GWGCWC 1 cut(s) 684
Alw26I GTCTC 1 cut(s) 510
AlwI GGATC 3 cut(s) 27, 108, 481
AlwNI CAGNNNCTG 1 cut(s) 713
AoxI GGCC 4 cut(s) 59, 73, 442, 576
ApeKI GCWGC 4 cut(s) 298, 710, 713, 725
AseI ATTAAT 1 cut(s) 396
AspLEI GCGC 1 cut(s) 640
AspS9I GGNCC 2 cut(s) 443, 577
AsuHPI GGTGA 1 cut(s) 529
BaeGI GKGCMC 1 cut(s) 465
Bbv12I GWGCWC 1 cut(s) 684
BbvI GCAGC 4 cut(s) 285, 697, 700, 712
BccI CCATC 1 cut(s) 400
BciT130I CCWGG 2 cut(s) 243, 252
BcoDI GTCTC 1 cut(s) 510
BfmI CTRYAG 2 cut(s) 438, 714
BisI GCNGC 4 cut(s) 299, 711, 714, 726
BlsI GCNGC 4 cut(s) 300, 712, 715, 727
Bme1390I CCNGG 2 cut(s) 243, 252
BmgT120I GGNCC 2 cut(s) 443, 577
BmrFI CCNGG 2 cut(s) 243, 252
BmrI ACTGGG 1 cut(s) 367
BmuI ACTGGG 1 cut(s) 367
BpmI CTGGAG 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 643
BsaI GGTCTC 1 cut(s) 510
BsaJI CCNNGG 4 cut(s) 62, 241, 486, 691
Bsc4I CCNNNNNNNGG 1 cut(s) 585
Bse1I ACTGG 2 cut(s) 362, 459
BseBI CCWGG 2 cut(s) 243, 252
BseDI CCNNGG 4 cut(s) 62, 241, 486, 691
BseGI GGATG 1 cut(s) 237
BseLI CCNNNNNNNGG 1 cut(s) 585
BseNI ACTGG 2 cut(s) 362, 459
BseSI GKGCMC 1 cut(s) 465
BseXI GCAGC 4 cut(s) 285, 697, 700, 712
BseYI CCCAGC 1 cut(s) 301
BshFI GGCC 4 cut(s) 61, 75, 444, 578
BsiHKAI GWGCWC 1 cut(s) 684
BslFI GGGAC 1 cut(s) 586
BslI CCNNNNNNNGG 1 cut(s) 585
BsmAI GTCTC 1 cut(s) 510
BsmFI GGGAC 1 cut(s) 586
BsnI GGCC 4 cut(s) 61, 75, 444, 578
Bso31I GGTCTC 1 cut(s) 510
Bsp1286I GDGCHC 2 cut(s) 465, 684
Bsp143I GATC 3 cut(s) 32, 100, 473
Bsp19I CCATGG 1 cut(s) 486
BspACI CCGC 1 cut(s) 604
BspANI GGCC 4 cut(s) 61, 75, 444, 578
BspMAI CTGCAG 2 cut(s) 442, 718
BspPI GGATC 3 cut(s) 27, 108, 481
BspTNI GGTCTC 1 cut(s) 510
BsrI ACTGG 2 cut(s) 362, 459
BssECI CCNNGG 4 cut(s) 62, 241, 486, 691
BssMI GATC 3 cut(s) 32, 100, 473
BssT1I CCWWGG 3 cut(s) 62, 486, 691
Bst2UI CCWGG 2 cut(s) 243, 252
Bst4CI ACNGT 1 cut(s) 325
BstC8I GCNNGC 2 cut(s) 442, 730
BstDSI CCRYGG 1 cut(s) 486
BstF5I GGATG 1 cut(s) 237
BstHHI GCGC 1 cut(s) 640
BstKTI GATC 3 cut(s) 35, 103, 476
BstMAI GTCTC 1 cut(s) 510
BstMBI GATC 3 cut(s) 32, 100, 473
BstMWI GCNNNNNNNGC 1 cut(s) 722
BstNI CCWGG 2 cut(s) 243, 252
BstSCI CCNGG 2 cut(s) 241, 250
BstSFI CTRYAG 2 cut(s) 438, 714
BstSLI GKGCMC 1 cut(s) 465
BstV1I GCAGC 4 cut(s) 285, 697, 700, 712
BsuRI GGCC 4 cut(s) 61, 75, 444, 578
BtgI CCRYGG 1 cut(s) 486
BtgZI GCGATG 1 cut(s) 183
BtsCI GGATG 1 cut(s) 237
Cac8I GCNNGC 2 cut(s) 442, 730
CaiI CAGNNNCTG 1 cut(s) 713
CfoI GCGC 1 cut(s) 640
Cfr13I GGNCC 2 cut(s) 443, 577
CseI GACGC 1 cut(s) 303
CviAII CATG 4 cut(s) 227, 365, 487, 702
DpnI GATC 3 cut(s) 34, 102, 475
DpnII GATC 3 cut(s) 32, 100, 473
DraIII CACNNNGTG 1 cut(s) 119
Eco130I CCWWGG 3 cut(s) 62, 486, 691
Eco31I GGTCTC 1 cut(s) 510
Eco57I CTGAAG 1 cut(s) 210
EcoO109I RGGNCCY 2 cut(s) 443, 577
EcoRII CCWGG 2 cut(s) 241, 250
EcoT14I CCWWGG 3 cut(s) 62, 486, 691
ErhI CCWWGG 3 cut(s) 62, 486, 691
FaeI CATG 4 cut(s) 230, 368, 490, 705
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FaqI GGGAC 1 cut(s) 586
FatI CATG 4 cut(s) 226, 364, 486, 701
FauI CCCGC 1 cut(s) 611
Fnu4HI GCNGC 4 cut(s) 299, 711, 714, 726
FokI GGATG 1 cut(s) 224
Fsp4HI GCNGC 4 cut(s) 299, 711, 714, 726
GlaI GCGC 1 cut(s) 639
GluI GCNGC 4 cut(s) 299, 711, 714, 726
GsaI CCCAGC 1 cut(s) 305
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 4 cut(s) 61, 75, 444, 578
HgaI GACGC 1 cut(s) 303
HhaI GCGC 1 cut(s) 640
Hin1II CATG 4 cut(s) 230, 368, 490, 705
Hin6I GCGC 1 cut(s) 638
HinP1I GCGC 1 cut(s) 638
HinfI GANTC 3 cut(s) 178, 563, 599
HphI GGTGA 1 cut(s) 529
Hpy166II GTNNAC 1 cut(s) 567
Hpy188I TCNGA 1 cut(s) 87
Hpy188III TCNNGA 1 cut(s) 509
Hpy8I GTNNAC 1 cut(s) 567
HpyAV CCTTC 1 cut(s) 363
HpyCH4III ACNGT 1 cut(s) 325
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 3 cut(s) 430, 440, 716
HpyF10VI GCNNNNNNNGC 1 cut(s) 722
HpySE526I ACGT 1 cut(s) 118
Hsp92II CATG 4 cut(s) 230, 368, 490, 705
HspAI GCGC 1 cut(s) 638
Kzo9I GATC 3 cut(s) 32, 100, 473
LmnI GCTCC 2 cut(s) 87, 361
Lsp1109I GCAGC 4 cut(s) 285, 697, 700, 712
MaeII ACGT 1 cut(s) 118
MaeIII GTNAC 4 cut(s) 179, 259, 319, 517
MalI GATC 3 cut(s) 34, 102, 475
MboI GATC 3 cut(s) 32, 100, 473
MboII GAAGA 2 cut(s) 154, 601
MhlI GDGCHC 2 cut(s) 465, 684
MluCI AATT 3 cut(s) 393, 425, 675
MlyI GAGTC 3 cut(s) 187, 572, 608
MmeI TCCRAC 2 cut(s) 65, 242
MnlI CCTC 5 cut(s) 48, 277, 495, 663, 715
MseI TTAA 4 cut(s) 204, 396, 554, 674
MslI CAYNNNNRTG 1 cut(s) 491
MspA1I CMGCKG 1 cut(s) 710
MspR9I CCNGG 2 cut(s) 243, 252
MvaI CCWGG 2 cut(s) 243, 252
MwoI GCNNNNNNNGC 1 cut(s) 722
NcoI CCATGG 1 cut(s) 486
NdeII GATC 3 cut(s) 32, 100, 473
NlaIII CATG 4 cut(s) 230, 368, 490, 705
NmuCI GTSAC 3 cut(s) 179, 319, 517
PkrI GCNGC 4 cut(s) 300, 712, 715, 727
PleI GAGTC 3 cut(s) 186, 571, 607
PpsI GAGTC 3 cut(s) 186, 571, 607
PshBI ATTAAT 1 cut(s) 396
PsiI TTATAA 1 cut(s) 270
Psp6I CCWGG 2 cut(s) 241, 250
PspFI CCCAGC 1 cut(s) 301
PspGI CCWGG 2 cut(s) 241, 250
PspPI GGNCC 2 cut(s) 443, 577
PstI CTGCAG 2 cut(s) 442, 718
PstNI CAGNNNCTG 1 cut(s) 713
PvuII CAGCTG 1 cut(s) 710
RseI CAYNNNNRTG 1 cut(s) 491
SaqAI TTAA 4 cut(s) 204, 396, 554, 674
SatI GCNGC 4 cut(s) 299, 711, 714, 726
Sau3AI GATC 3 cut(s) 32, 100, 473
Sau96I GGNCC 2 cut(s) 443, 577
SchI GAGTC 3 cut(s) 187, 572, 608
ScrFI CCNGG 2 cut(s) 243, 252
SduI GDGCHC 2 cut(s) 465, 684
SfcI CTRYAG 2 cut(s) 438, 714
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 1 cut(s) 622
SmoI CTYRAG 1 cut(s) 622
Sse9I AATT 3 cut(s) 393, 425, 675
SsiI CCGC 1 cut(s) 604
StyD4I CCNGG 2 cut(s) 241, 250
StyI CCWWGG 3 cut(s) 62, 486, 691
TaaI ACNGT 1 cut(s) 325
TaiI ACGT 1 cut(s) 121
TaqI TCGA 2 cut(s) 136, 176
TasI AATT 3 cut(s) 393, 425, 675
Tru1I TTAA 4 cut(s) 204, 396, 554, 674
Tru9I TTAA 4 cut(s) 204, 396, 554, 674
TseFI GTSAC 3 cut(s) 179, 319, 517
TseI GCWGC 4 cut(s) 298, 710, 713, 725
Tsp45I GTSAC 3 cut(s) 179, 319, 517
TspDTI ATGAA 3 cut(s) 4, 215, 403
TspGWI ACGGA 1 cut(s) 531
VspI ATTAAT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.