Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003932

Physical Location & Seq

Genomic Coordinates Reverse (-)

16828051 .. 16831092

Gene Structure 3042 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1106500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1038 bp

ATGGATAGAGAATGGGGTTCAAAGCCCGGCAGCGGCGGCGCCGCCTCTGCACAGAACGAAGCCATCGACCGCCGGGAGCGTCTCCGGCGACTCGCTCTCGAGACCATCGATCTCGCCAAAGATCCCTATTTCATGCGCAATCACCTCGGAAGCTATGAATGTAAGCTTTGCTTGACGCTGCACAACAACGAGGGCAACTACTTGGCGCATACACAAGGCAAGCGCCATCAGACCAACTTGGCGAAACGAGCTGCTCGCGAGGCAAAGGACGCTCCTGCTCAGCCCCAGCCGCACAAGCGCAAGGTCAATGTGCGAAAGACGGTCAAAATTGGTAGGCCGGGGTATCGAGTGACAAAGCAGTTTGATGGAGAAACAAAGCAGAGGTCTCTTCTCTTCCAGATTGAATATCCTGAGATTGAAGATAATACAAAGCCAAGGCACCGTTTTATGTCATCTTATGAGCAGAGGGTTCAACCTGTGGACAAAAGGTACCAGTATCTTCTATTTGCAGCCGAGCCTTACGAGATAGTTGCTTTCAAGGTTCCTAACATTGAGATTGACAAAACTGATAAGTTTTTCTCACATTGGGATCCAGACTCAAAAATGTTCACATTGCAGCTATATTTCAAAACTAAGCCACCAGAGGCGAACAAACCTCAACCTCCCCCTGCAGCCAATGGCCCAGGTGCTCCCGGCGTTCCATCAAGGCCTTTGCCTCCACCACCCCAAGGTCCTCCTCCTCCTCCACCACAAGGACTGCCGCCTGGTGCACCAATGGGAAATCCTCCCAGAGCTCCTCCGCCAATTCCTGGATCTTTACTACCATCAGCTCCTATGGGAAATGGCCCTAGGCCTATGCCTCCTGGTGGTGCTCCACCTGCCCCTCCTCCGCCCTCTGTTGGTAACACAATGGTGAATTTCACTCCTGGCACTCAGATGGGAAGGCCTCCATCAATGCCACCTCCACAAGGCTTTCCGGGACAACAAATGCAGGGCCAGGGTCTTCGTCCACCCCCACCACCTCCTAATATGAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000245 GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005681 GO:0005684 GO:0005686 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0007275 GO:0007399 GO:0008150 GO:0008152 GO:0008380 GO:0009987 GO:0010467 GO:0010720 GO:0010975 GO:0010976 GO:0016043 GO:0016070 GO:0016071 GO:0016604 GO:0016607 GO:0022008 GO:0022607 GO:0022613 GO:0022618 GO:0030154 GO:0030532 GO:0031344 GO:0031346 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034622 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0046483 GO:0048518 GO:0048522 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051128 GO:0051130 GO:0051239 GO:0051240 GO:0051960 GO:0051962 GO:0060284 GO:0065003 GO:0065007 GO:0070013 GO:0071004 GO:0071010 GO:0071013 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097159 GO:0097525 GO:0120035 GO:0120114 GO:1901360 GO:1901363 GO:1902494 GO:1990904 GO:2000026

KEGG Pathways

Metabolic & Signaling

ko03040 map03040

Pfam Domains

Protein Families

SF3A2 zf-met

Protein Analysis

346

Amino Acids

37.98

Weight (kDa)

9.92

Isoelectric Point (pI)

65.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-met	PF12874	52 - 76	1.1e-06	Zinc-finger of C2H2 type
SF3A2	PF16835	110 - 203	1e-36	Pre-mRNA-splicing factor SF3a complex subunit 2 (Prp11)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003932)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32600
fragaria_vesca	FvH4_6g16880 FvH4_6g16880 FvH4_6g16880 FvH4_6g16880
malus_domestica	MD04G1088800.v1.1 MD12G1106500.v1.1
prunus_persica	Prupe.4G071900_v2.0.a1 Prupe.7G125300_v2.0.a1 Prupe.7G125300_v2.0.a1
pyrus_communis	pycom04g08310 pycom12g10700
rosa_chinensis	RchiOBHm_Chr3g0470901 RchiOBHm_Chr7g0191101
rosa_laevigata	RLG00000024235 RLG00000025908
rosa_multiflora	Rmu_co8030388.1_g000001 Rmu_sc0005903.1_g000001 Rmu_sc0010267.1_g000002 Rmu_sc0030335.1_g000001
rosa_roxburghii	Rroxscaffold_3G00264030 Rroxscaffold_6G00410430 Rroxscaffold_6G00426670
rosa_rugosa	Rorug03G0113500 Rorug06G0508500
rosa_samantha	Rh1AG146000 Rh1DG152700 Rh3AG163300 Rh3BG188700 Rh3CG178700 Rh3DG187400 Rh7BG118500 Rh7CG121000
rosa_wichuraiana	Rw3G015380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	886
Acc16I	TGCGCA	1 cut(s)	137
Acc36I	ACCTGC	1 cut(s)	886
Acc65I	GGTACC	1 cut(s)	489
AccB1I	GGYRCC	3 cut(s)	38, 438, 489
AccB7I	CCANNNNNTGG	1 cut(s)	809
AccII	CGCG	1 cut(s)	258
AciI	CCGC	8 cut(s)	33, 36, 42, 70, 290, 761, 800, 890
AclWI	GGATC	4 cut(s)	116, 584, 597, 820
AcsI	RAATTY	1 cut(s)	916
AcyI	GRCGYC	1 cut(s)	39
AfaI	GTAC	1 cut(s)	491
AfiI	CCNNNNNNNGG	7 cut(s)	32, 728, 752, 809, 866, 899, 968
AgsI	TTSAA	6 cut(s)	21, 404, 419, 473, 538, 628
AjnI	CCWGG	6 cut(s)	682, 763, 808, 862, 925, 996
AleI	CACNNNNGTG	1 cut(s)	911
AluBI	AGCT	6 cut(s)	153, 166, 251, 619, 794, 830
AluI	AGCT	6 cut(s)	153, 166, 251, 619, 794, 830
Alw21I	GWGCWC	4 cut(s)	691, 772, 796, 874
Alw26I	GTCTC	3 cut(s)	86, 95, 390
Alw44I	GTGCAC	1 cut(s)	768
AlwI	GGATC	4 cut(s)	116, 584, 597, 820
Ama87I	CYCGRG	1 cut(s)	98
AoxI	GGCC	7 cut(s)	335, 679, 707, 844, 851, 944, 994
ApaLI	GTGCAC	1 cut(s)	768
ApeKI	GCWGC	6 cut(s)	30, 178, 251, 509, 616, 671
ApoI	RAATTY	1 cut(s)	916
ArsI	GACNNNNNNTTYG	2 cut(s)	368, 400
Asp718I	GGTACC	1 cut(s)	489
AspA2I	CCTAGG	1 cut(s)	848
AspLEI	GCGC	5 cut(s)	41, 138, 208, 225, 300
AspS9I	GGNCC	4 cut(s)	680, 731, 845, 994
AsuC2I	CCSGG	5 cut(s)	27, 74, 339, 693, 978
AsuHPI	GGTGA	2 cut(s)	134, 925
AvaI	CYCGRG	1 cut(s)	98
AvaII	GGWCC	1 cut(s)	731
AvrII	CCTAGG	1 cut(s)	848
BaeGI	GKGCMC	1 cut(s)	772
BamHI	GGATCC	1 cut(s)	589
BanI	GGYRCC	3 cut(s)	38, 438, 489
BanII	GRGCYC	1 cut(s)	796
BbsI	GAAGAC	1 cut(s)	995
Bbv12I	GWGCWC	4 cut(s)	691, 772, 796, 874
BbvI	GCAGC	6 cut(s)	42, 165, 238, 521, 628, 683
BccI	CCATC	8 cut(s)	71, 113, 234, 359, 709, 832, 931, 958
BcgI	CGANNNNNNTGC	4 cut(s)	127, 161, 512, 546
BciT130I	CCWGG	6 cut(s)	684, 765, 810, 864, 927, 998
BcnI	CCSGG	5 cut(s)	27, 74, 339, 693, 978
BcoDI	GTCTC	3 cut(s)	86, 95, 390
BfaI	CTAG	1 cut(s)	849
BfmI	CTRYAG	1 cut(s)	669
BfoI	RGCGCY	2 cut(s)	42, 226
BfuAI	ACCTGC	1 cut(s)	886
BlnI	CCTAGG	1 cut(s)	848
BlpI	GCTNAGC	1 cut(s)	279
Bme18I	GGWCC	1 cut(s)	731
BmeT110I	CYCGRG	1 cut(s)	98
BmgT120I	GGNCC	4 cut(s)	680, 731, 845, 994
BmiI	GGNNCC	5 cut(s)	40, 440, 491, 543, 591
BpiI	GAAGAC	1 cut(s)	995
Bpu1102I	GCTNAGC	1 cut(s)	279
BpuMI	CCSGG	5 cut(s)	27, 74, 339, 693, 978
Bsa29I	ATCGAT	1 cut(s)	108
BsaHI	GRCGYC	1 cut(s)	39
BsaI	GGTCTC	2 cut(s)	95, 390
BsaJI	CCNNGG	7 cut(s)	145, 338, 434, 682, 727, 848, 997
Bsc4I	CCNNNNNNNGG	7 cut(s)	32, 728, 752, 809, 866, 899, 968
Bse1I	ACTGG	1 cut(s)	493
Bse3DI	GCAATG	1 cut(s)	611
BseBI	CCWGG	6 cut(s)	684, 765, 810, 864, 927, 998
BseCI	ATCGAT	1 cut(s)	108
BseDI	CCNNGG	7 cut(s)	145, 338, 434, 682, 727, 848, 997
BseLI	CCNNNNNNNGG	7 cut(s)	32, 728, 752, 809, 866, 899, 968
BseMI	GCAATG	1 cut(s)	611
BseMII	CTCAG	3 cut(s)	293, 402, 947
BseNI	ACTGG	1 cut(s)	493
BseRI	GAGGAG	5 cut(s)	726, 729, 732, 786, 876
BseSI	GKGCMC	1 cut(s)	772
BseXI	GCAGC	6 cut(s)	42, 165, 238, 521, 628, 683
BseYI	CCCAGC	1 cut(s)	285
BsgI	GTGCAG	2 cut(s)	33, 164
Bsh1236I	CGCG	1 cut(s)	258
Bsh1285I	CGRYCG	1 cut(s)	70
BshFI	GGCC	7 cut(s)	337, 681, 709, 846, 853, 946, 996
BshNI	GGYRCC	3 cut(s)	38, 438, 489
BshVI	ATCGAT	1 cut(s)	108
BsiEI	CGRYCG	1 cut(s)	70
BsiHKAI	GWGCWC	4 cut(s)	691, 772, 796, 874
BsiHKCI	CYCGRG	1 cut(s)	98
BsiSI	CCGG	6 cut(s)	27, 73, 85, 338, 693, 977
BslFI	GGGAC	1 cut(s)	993
BslI	CCNNNNNNNGG	7 cut(s)	32, 728, 752, 809, 866, 899, 968
BsmAI	GTCTC	3 cut(s)	86, 95, 390
BsmBI	CGTCTC	1 cut(s)	86
BsmFI	GGGAC	1 cut(s)	993
BsnI	GGCC	7 cut(s)	337, 681, 709, 846, 853, 946, 996
Bso31I	GGTCTC	2 cut(s)	95, 390
BsoBI	CYCGRG	1 cut(s)	98
Bsp1286I	GDGCHC	4 cut(s)	691, 772, 796, 874
Bsp143I	GATC	4 cut(s)	109, 121, 589, 812
Bsp1720I	GCTNAGC	1 cut(s)	279
Bsp68I	TCGCGA	1 cut(s)	258
BspACI	CCGC	8 cut(s)	33, 36, 42, 70, 290, 761, 800, 890
BspANI	GGCC	7 cut(s)	337, 681, 709, 846, 853, 946, 996
BspCNI	CTCAG	3 cut(s)	292, 403, 946
BspDI	ATCGAT	1 cut(s)	108
BspFNI	CGCG	1 cut(s)	258
BspLI	GGNNCC	5 cut(s)	40, 440, 491, 543, 591
BspMAI	CTGCAG	1 cut(s)	673
BspMI	ACCTGC	1 cut(s)	886
BspPI	GGATC	4 cut(s)	116, 584, 597, 820
BspT107I	GGYRCC	3 cut(s)	38, 438, 489
BspTNI	GGTCTC	2 cut(s)	95, 390
BsrDI	GCAATG	1 cut(s)	611
BsrI	ACTGG	1 cut(s)	493
BssECI	CCNNGG	7 cut(s)	145, 338, 434, 682, 727, 848, 997
BssMI	GATC	4 cut(s)	109, 121, 589, 812
BssNI	GRCGYC	1 cut(s)	39
BssT1I	CCWWGG	3 cut(s)	434, 727, 848
Bst2UI	CCWGG	6 cut(s)	684, 765, 810, 864, 927, 998
Bst4CI	ACNGT	2 cut(s)	322, 443
Bst6I	CTCTTC	2 cut(s)	393, 398
BstACI	GRCGYC	1 cut(s)	39
BstC8I	GCNNGC	2 cut(s)	221, 256
BstDEI	CTNAG	4 cut(s)	279, 411, 633, 933
BstENI	CCTNNNNNAGG	1 cut(s)	966
BstFNI	CGCG	1 cut(s)	258
BstH2I	RGCGCY	2 cut(s)	42, 226
BstHHI	GCGC	5 cut(s)	41, 138, 208, 225, 300
BstKTI	GATC	4 cut(s)	112, 124, 592, 815
BstMAI	GTCTC	3 cut(s)	86, 95, 390
BstMBI	GATC	4 cut(s)	109, 121, 589, 812
BstMCI	CGRYCG	1 cut(s)	70
BstMWI	GCNNNNNNNGC	9 cut(s)	36, 47, 85, 248, 260, 269, 289, 295, 878
BstNI	CCWGG	6 cut(s)	684, 765, 810, 864, 927, 998
BstSFI	CTRYAG	1 cut(s)	669
BstSLI	GKGCMC	1 cut(s)	772
BstUI	CGCG	1 cut(s)	258
BstV1I	GCAGC	6 cut(s)	42, 165, 238, 521, 628, 683
BstV2I	GAAGAC	1 cut(s)	995
BstX2I	RGATCY	3 cut(s)	121, 589, 812
BstYI	RGATCY	3 cut(s)	121, 589, 812
Bsu15I	ATCGAT	1 cut(s)	108
BsuRI	GGCC	7 cut(s)	337, 681, 709, 846, 853, 946, 996
BsuTUI	ATCGAT	1 cut(s)	108
BtuMI	TCGCGA	1 cut(s)	258
BveI	ACCTGC	1 cut(s)	886
Cac8I	GCNNGC	2 cut(s)	221, 256
CfoI	GCGC	5 cut(s)	41, 138, 208, 225, 300
Cfr13I	GGNCC	4 cut(s)	680, 731, 845, 994
ClaI	ATCGAT	1 cut(s)	108
CseI	GACGC	3 cut(s)	68, 184, 278
Csp6I	GTAC	1 cut(s)	490
CviAII	CATG	1 cut(s)	133
CviQI	GTAC	1 cut(s)	490
DdeI	CTNAG	4 cut(s)	279, 411, 633, 933
DinI	GGCGCC	1 cut(s)	40
DpnI	GATC	4 cut(s)	111, 123, 591, 814
DpnII	GATC	4 cut(s)	109, 121, 589, 812
Eam1104I	CTCTTC	2 cut(s)	393, 398
EarI	CTCTTC	2 cut(s)	393, 398
EciI	GGCGGA	2 cut(s)	789, 879
Ecl136II	GAGCTC	1 cut(s)	794
Eco130I	CCWWGG	3 cut(s)	434, 727, 848
Eco147I	AGGCCT	3 cut(s)	709, 853, 946
Eco24I	GRGCYC	1 cut(s)	796
Eco31I	GGTCTC	2 cut(s)	95, 390
Eco47I	GGWCC	1 cut(s)	731
Eco53kI	GAGCTC	1 cut(s)	794
Eco88I	CYCGRG	1 cut(s)	98
EcoICRI	GAGCTC	1 cut(s)	794
EcoNI	CCTNNNNNAGG	1 cut(s)	966
EcoO109I	RGGNCCY	1 cut(s)	731
EcoRII	CCWGG	6 cut(s)	682, 763, 808, 862, 925, 996
EcoT14I	CCWWGG	3 cut(s)	434, 727, 848
EcoT38I	GRGCYC	1 cut(s)	796
EgeI	GGCGCC	1 cut(s)	40
EheI	GGCGCC	1 cut(s)	40
ErhI	CCWWGG	3 cut(s)	434, 727, 848
Esp3I	CGTCTC	1 cut(s)	86
FaeI	CATG	1 cut(s)	136
FaiI	YATR	9 cut(s)	134, 156, 210, 449, 459, 622, 836, 857, 1031
FalI	AAGNNNNNCTT	2 cut(s)	155, 187
FaqI	GGGAC	1 cut(s)	993
FatI	CATG	1 cut(s)	132
FriOI	GRGCYC	1 cut(s)	796
FspBI	CTAG	1 cut(s)	849
FspI	TGCGCA	1 cut(s)	137
GlaI	GCGC	5 cut(s)	40, 137, 207, 224, 299
GsaI	CCCAGC	1 cut(s)	289
HaeII	RGCGCY	2 cut(s)	42, 226
HaeIII	GGCC	7 cut(s)	337, 681, 709, 846, 853, 946, 996
HapII	CCGG	6 cut(s)	27, 73, 85, 338, 693, 977
HgaI	GACGC	3 cut(s)	68, 184, 278
HhaI	GCGC	5 cut(s)	41, 138, 208, 225, 300
Hin1I	GRCGYC	1 cut(s)	39
Hin1II	CATG	1 cut(s)	136
Hin6I	GCGC	5 cut(s)	39, 136, 206, 223, 298
HinP1I	GCGC	5 cut(s)	39, 136, 206, 223, 298
HindIII	AAGCTT	1 cut(s)	164
HinfI	GANTC	2 cut(s)	90, 596
HpaII	CCGG	6 cut(s)	27, 73, 85, 338, 693, 977
HphI	GGTGA	2 cut(s)	134, 925
Hpy166II	GTNNAC	4 cut(s)	481, 609, 770, 1010
Hpy188I	TCNGA	3 cut(s)	149, 231, 936
Hpy188III	TCNNGA	6 cut(s)	98, 100, 257, 397, 410, 593
Hpy8I	GTNNAC	4 cut(s)	481, 609, 770, 1010
HpyAV	CCTTC	1 cut(s)	936
HpyCH4III	ACNGT	2 cut(s)	322, 443
HpyCH4V	TGCA	7 cut(s)	50, 181, 509, 616, 671, 770, 991
HpyF10VI	GCNNNNNNNGC	9 cut(s)	36, 47, 85, 248, 260, 269, 289, 295, 878
HpyF3I	CTNAG	4 cut(s)	279, 411, 633, 933
Hsp92I	GRCGYC	1 cut(s)	39
Hsp92II	CATG	1 cut(s)	136
HspAI	GCGC	5 cut(s)	39, 136, 206, 223, 298
KasI	GGCGCC	1 cut(s)	38
KpnI	GGTACC	1 cut(s)	493
Kzo9I	GATC	4 cut(s)	109, 121, 589, 812
LmnI	GCTCC	6 cut(s)	76, 277, 694, 799, 835, 877
Lsp1109I	GCAGC	6 cut(s)	42, 165, 238, 521, 628, 683
MaeI	CTAG	1 cut(s)	849
MaeIII	GTNAC	2 cut(s)	349, 902
MalI	GATC	4 cut(s)	111, 123, 591, 814
MboI	GATC	4 cut(s)	109, 121, 589, 812
MboII	GAAGA	5 cut(s)	380, 385, 431, 491, 995
MflI	RGATCY	3 cut(s)	121, 589, 812
MhlI	GDGCHC	4 cut(s)	691, 772, 796, 874
MluCI	AATT	3 cut(s)	327, 804, 916
Mly113I	GGCGCC	1 cut(s)	39
MlyI	GAGTC	2 cut(s)	84, 590
MseI	TTAA	1 cut(s)	1036
MslI	CAYNNNNRTG	2 cut(s)	911, 935
MspA1I	CMGCKG	1 cut(s)	33
MspI	CCGG	6 cut(s)	27, 73, 85, 338, 693, 977
MvaI	CCWGG	6 cut(s)	684, 765, 810, 864, 927, 998
MvnI	CGCG	1 cut(s)	258
MwoI	GCNNNNNNNGC	9 cut(s)	36, 47, 85, 248, 260, 269, 289, 295, 878
NarI	GGCGCC	1 cut(s)	39
NciI	CCSGG	5 cut(s)	27, 74, 339, 693, 978
NdeII	GATC	4 cut(s)	109, 121, 589, 812
NlaIII	CATG	1 cut(s)	136
NlaIV	GGNNCC	5 cut(s)	40, 440, 491, 543, 591
NmeAIII	GCCGAG	1 cut(s)	538
NmuCI	GTSAC	1 cut(s)	349
NruI	TCGCGA	1 cut(s)	258
NsbI	TGCGCA	1 cut(s)	137
OliI	CACNNNNGTG	1 cut(s)	911
PaeR7I	CTCGAG	1 cut(s)	98
PaqCI	CACCTGC	1 cut(s)	886
PceI	AGGCCT	3 cut(s)	709, 853, 946
PcsI	WCGNNNNNNNCGW	2 cut(s)	63, 105
PflMI	CCANNNNNTGG	1 cut(s)	809
PfoI	TCCNGGA	2 cut(s)	808, 976
PleI	GAGTC	2 cut(s)	84, 590
PluTI	GGCGCC	1 cut(s)	42
PpsI	GAGTC	2 cut(s)	84, 590
PpuMI	RGGWCCY	1 cut(s)	731
Psp124BI	GAGCTC	1 cut(s)	796
Psp5II	RGGWCCY	1 cut(s)	731
Psp6I	CCWGG	6 cut(s)	682, 763, 808, 862, 925, 996
PspFI	CCCAGC	1 cut(s)	285
PspGI	CCWGG	6 cut(s)	682, 763, 808, 862, 925, 996
PspN4I	GGNNCC	5 cut(s)	40, 440, 491, 543, 591
PspPI	GGNCC	4 cut(s)	680, 731, 845, 994
PspPPI	RGGWCCY	1 cut(s)	731
PstI	CTGCAG	1 cut(s)	673
PsuI	RGATCY	3 cut(s)	121, 589, 812
RruI	TCGCGA	1 cut(s)	258
RsaI	GTAC	1 cut(s)	491
RsaNI	GTAC	1 cut(s)	490
RseI	CAYNNNNRTG	2 cut(s)	911, 935
SacI	GAGCTC	1 cut(s)	796
SaqAI	TTAA	1 cut(s)	1036
Sau3AI	GATC	4 cut(s)	109, 121, 589, 812
Sau96I	GGNCC	4 cut(s)	680, 731, 845, 994
SchI	GAGTC	2 cut(s)	84, 590
SduI	GDGCHC	4 cut(s)	691, 772, 796, 874
SfcI	CTRYAG	1 cut(s)	669
SfoI	GGCGCC	1 cut(s)	40
Sfr274I	CTCGAG	1 cut(s)	98
SinI	GGWCC	1 cut(s)	731
SlaI	CTCGAG	1 cut(s)	98
SmiMI	CAYNNNNRTG	2 cut(s)	911, 935
SmlI	CTYRAG	1 cut(s)	98
SmoI	CTYRAG	1 cut(s)	98
Sse9I	AATT	3 cut(s)	327, 804, 916
SseBI	AGGCCT	3 cut(s)	709, 853, 946
SsiI	CCGC	8 cut(s)	33, 36, 42, 70, 290, 761, 800, 890
SspDI	GGCGCC	1 cut(s)	38
SspMI	CTAG	1 cut(s)	849
SstI	GAGCTC	1 cut(s)	796
StuI	AGGCCT	3 cut(s)	709, 853, 946
StyI	CCWWGG	3 cut(s)	434, 727, 848
TaaI	ACNGT	2 cut(s)	322, 443
TaqI	TCGA	4 cut(s)	66, 99, 108, 346
TasI	AATT	3 cut(s)	327, 804, 916
TauI	GCSGC	5 cut(s)	36, 39, 44, 292, 763
Tru1I	TTAA	1 cut(s)	1036
Tru9I	TTAA	1 cut(s)	1036
TseFI	GTSAC	1 cut(s)	349
TseI	GCWGC	6 cut(s)	30, 178, 251, 509, 616, 671
Tsp45I	GTSAC	1 cut(s)	349
TspDTI	ATGAA	2 cut(s)	121, 171
Van91I	CCANNNNNTGG	1 cut(s)	809
VneI	GTGCAC	1 cut(s)	768
VpaK11BI	GGWCC	1 cut(s)	731
XagI	CCTNNNNNAGG	1 cut(s)	966
XapI	RAATTY	1 cut(s)	916
XhoI	CTCGAG	1 cut(s)	98
XmaJI	CCTAGG	1 cut(s)	848
XspI	CTAG	1 cut(s)	849

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.