MD12G1159500.v1.1

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005403
Physical Location & Seq
Reverse (-)
23945668 .. 23947915
2248 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1159500.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGCGGAGTCAGTTCTCGAGCAGTTGAGGAATGGAACTGCTCGGTTCGAGCTCGCCTCCTCCCCCGTGCTTTCAATTTCAACTTCCAATTCTCAGTCGCACCAAAGGGCGGCGTTCGGGGACCACAGCCACCGGTTCTTCGCTAGAATCGGACCGCCACTAGGCAGAGGATCGTCGGCGATGAAGAAAGTTGAACGCTTTTCGGTTCAGAATGTTACCGGCGACGGGCGCTGTCTATTTCGTGCTCTGGTGAAAGGAATGGCGTTGAATAAAGGGGTTCCTCTTAGCCCAAGGCAAGAGAAAGATGATGCAGACGAGCTACGGATGGCTGTGAAAGAGGTTATATGCGAAGATGACGAAGAGCGCCTTAAGTATGAACCTGCTCTGGTGGCAATCACTGTTGACGAGTCCTTAAAACGTTACTGCCAGCGCATCAATAGACCTGATTTCTGGGGAGGAGAGTCTGAGCTGCTGGTCATGATTTATATGAGTGTGCTGTCAAAGTTGTGTGGGCAGCCAATCACTGTTTACATACCAGAGCATGAGCATACAAATGGTGGACGGGGTTCTGGTTTCATTCCGATTGCAGAATATGGAAGCGAGTTTACAAAAGGTTCTAGAAACAAAAAGCCCAGAAAGGTTGTGAGGCTCCTCTACAGTGGCAGGAACCATTATGATCTGCTTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

230

Amino Acids

25.64

Weight (kDa)

9.03

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 74 - 201 1.1e-08 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 388
AciI CCGC 3 cut(s) 5, 110, 155
AclI AACGTT 1 cut(s) 418
AclWI GGATC 1 cut(s) 178
AfiI CCNNNNNNNGG 3 cut(s) 109, 161, 225
AflII CTTAAG 1 cut(s) 368
AgeI ACCGGT 1 cut(s) 132
AgsI TTSAA 4 cut(s) 75, 81, 194, 268
AluBI AGCT 3 cut(s) 52, 319, 469
AluI AGCT 3 cut(s) 52, 319, 469
Alw21I GWGCWC 2 cut(s) 54, 247
AlwI GGATC 1 cut(s) 178
Ama87I CYCGRG 1 cut(s) 17
ApeKI GCWGC 2 cut(s) 469, 514
AsiGI ACCGGT 1 cut(s) 132
AspLEI GCGC 3 cut(s) 231, 366, 432
AspS9I GGNCC 2 cut(s) 121, 152
AsuHPI GGTGA 1 cut(s) 262
AvaI CYCGRG 1 cut(s) 17
AvaII GGWCC 2 cut(s) 121, 152
BanII GRGCYC 1 cut(s) 54
BarI GAAGNNNNNNTAC 2 cut(s) 589, 621
Bbv12I GWGCWC 2 cut(s) 54, 247
BbvI GCAGC 2 cut(s) 456, 526
BccI CCATC 1 cut(s) 319
BfaI CTAG 3 cut(s) 144, 161, 618
BfmI CTRYAG 1 cut(s) 655
BfoI RGCGCY 2 cut(s) 232, 367
BfrI CTTAAG 1 cut(s) 368
BfuAI ACCTGC 1 cut(s) 388
BisI GCNGC 3 cut(s) 111, 470, 515
BlsI GCNGC 3 cut(s) 112, 471, 516
Bme18I GGWCC 2 cut(s) 121, 152
BmeT110I CYCGRG 1 cut(s) 17
BmgT120I GGNCC 2 cut(s) 121, 152
BmiI GGNNCC 4 cut(s) 122, 279, 650, 668
BmsI GCATC 2 cut(s) 298, 441
BplI GAGNNNNNCTC 2 cut(s) 41, 73
BsaJI CCNNGG 1 cut(s) 290
BsaWI WCCGGW 1 cut(s) 132
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 161, 225
Bse118I RCCGGY 2 cut(s) 132, 218
BseDI CCNNGG 1 cut(s) 290
BseGI GGATG 1 cut(s) 330
BseLI CCNNNNNNNGG 3 cut(s) 109, 161, 225
BseMII CTCAG 2 cut(s) 107, 456
BseRI GAGGAG 3 cut(s) 49, 471, 641
BseXI GCAGC 2 cut(s) 456, 526
BshTI ACCGGT 1 cut(s) 132
BsiHKAI GWGCWC 2 cut(s) 54, 247
BsiHKCI CYCGRG 1 cut(s) 17
BsiSI CCGG 2 cut(s) 133, 219
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 3 cut(s) 109, 161, 225
BsmFI GGGAC 1 cut(s) 134
BsoBI CYCGRG 1 cut(s) 17
Bsp1286I GDGCHC 2 cut(s) 54, 247
Bsp143I GATC 2 cut(s) 170, 676
BspACI CCGC 3 cut(s) 5, 110, 155
BspCNI CTCAG 2 cut(s) 106, 457
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 4 cut(s) 122, 279, 650, 668
BspMI ACCTGC 1 cut(s) 388
BspPI GGATC 1 cut(s) 178
BspQI GCTCTTC 1 cut(s) 354
BspTI CTTAAG 1 cut(s) 368
BsrFI RCCGGY 2 cut(s) 132, 218
BssAI RCCGGY 2 cut(s) 132, 218
BssECI CCNNGG 1 cut(s) 290
BssMI GATC 2 cut(s) 170, 676
BssT1I CCWWGG 1 cut(s) 290
Bst4CI ACNGT 3 cut(s) 400, 526, 659
Bst6I CTCTTC 1 cut(s) 354
BstAFI CTTAAG 1 cut(s) 368
BstC8I GCNNGC 2 cut(s) 54, 428
BstDEI CTNAG 3 cut(s) 93, 284, 465
BstF5I GGATG 1 cut(s) 330
BstH2I RGCGCY 2 cut(s) 232, 367
BstHHI GCGC 3 cut(s) 231, 366, 432
BstKTI GATC 2 cut(s) 173, 679
BstMBI GATC 2 cut(s) 170, 676
BstMWI GCNNNNNNNGC 2 cut(s) 228, 389
BstSFI CTRYAG 1 cut(s) 655
BstV1I GCAGC 2 cut(s) 456, 526
BtgZI GCGATG 1 cut(s) 194
BtsCI GGATG 1 cut(s) 330
BtsIMutI CAGTG 3 cut(s) 396, 522, 664
BveI ACCTGC 1 cut(s) 388
Cac8I GCNNGC 2 cut(s) 54, 428
CciI TCATGA 1 cut(s) 477
CfoI GCGC 3 cut(s) 231, 366, 432
Cfr10I RCCGGY 2 cut(s) 132, 218
Cfr13I GGNCC 2 cut(s) 121, 152
CpoI CGGWCCG 1 cut(s) 152
CspAI ACCGGT 1 cut(s) 132
CspI CGGWCCG 1 cut(s) 152
CviAII CATG 2 cut(s) 478, 542
CviJI RGCY 9 cut(s) 52, 129, 288, 319, 329, 469, 517, 631, 649
CviKI_1 RGCY 9 cut(s) 52, 129, 288, 319, 329, 469, 517, 631, 649
DdeI CTNAG 3 cut(s) 93, 284, 465
DpnI GATC 2 cut(s) 172, 678
DpnII GATC 2 cut(s) 170, 676
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 52
Eco130I CCWWGG 1 cut(s) 290
Eco24I GRGCYC 1 cut(s) 54
Eco47I GGWCC 2 cut(s) 121, 152
Eco53kI GAGCTC 1 cut(s) 52
Eco88I CYCGRG 1 cut(s) 17
EcoICRI GAGCTC 1 cut(s) 52
EcoT14I CCWWGG 1 cut(s) 290
EcoT38I GRGCYC 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 2 cut(s) 481, 545
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FaqI GGGAC 1 cut(s) 134
FatI CATG 2 cut(s) 477, 541
Fnu4HI GCNGC 3 cut(s) 111, 470, 515
FokI GGATG 1 cut(s) 337
FriOI GRGCYC 1 cut(s) 54
Fsp4HI GCNGC 3 cut(s) 111, 470, 515
FspBI CTAG 3 cut(s) 144, 161, 618
GlaI GCGC 3 cut(s) 230, 365, 431
GluI GCNGC 3 cut(s) 111, 470, 515
HaeII RGCGCY 2 cut(s) 232, 367
HapII CCGG 2 cut(s) 133, 219
HhaI GCGC 3 cut(s) 231, 366, 432
Hin1II CATG 2 cut(s) 481, 545
Hin6I GCGC 3 cut(s) 229, 364, 430
HinP1I GCGC 3 cut(s) 229, 364, 430
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HinfI GANTC 4 cut(s) 8, 147, 407, 461
HpaII CCGG 2 cut(s) 133, 219
HphI GGTGA 1 cut(s) 262
Hpy166II GTNNAC 4 cut(s) 403, 529, 560, 606
Hpy188I TCNGA 4 cut(s) 152, 210, 466, 582
Hpy188III TCNNGA 3 cut(s) 17, 478, 618
Hpy8I GTNNAC 4 cut(s) 403, 529, 560, 606
Hpy99I CGWCG 2 cut(s) 178, 227
HpyCH4III ACNGT 3 cut(s) 400, 526, 659
HpyCH4IV ACGT 1 cut(s) 418
HpyCH4V TGCA 2 cut(s) 311, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 228, 389
HpyF3I CTNAG 3 cut(s) 93, 284, 465
HpySE526I ACGT 1 cut(s) 418
Hsp92II CATG 2 cut(s) 481, 545
HspAI GCGC 3 cut(s) 229, 364, 430
Kzo9I GATC 2 cut(s) 170, 676
LguI GCTCTTC 1 cut(s) 354
LmnI GCTCC 1 cut(s) 654
Lsp1109I GCAGC 2 cut(s) 456, 526
LweI GCATC 2 cut(s) 298, 441
MaeI CTAG 3 cut(s) 144, 161, 618
MaeII ACGT 1 cut(s) 418
MaeIII GTNAC 2 cut(s) 214, 419
MalI GATC 2 cut(s) 172, 678
MboI GATC 2 cut(s) 170, 676
MboII GAAGA 4 cut(s) 130, 196, 362, 371
MhlI GDGCHC 2 cut(s) 54, 247
MluCI AATT 2 cut(s) 75, 88
MlyI GAGTC 3 cut(s) 17, 416, 470
MnlI CCTC 9 cut(s) 21, 67, 70, 161, 291, 331, 449, 639, 662
MseI TTAA 2 cut(s) 369, 413
MslI CAYNNNNRTG 1 cut(s) 552
MspCI CTTAAG 1 cut(s) 368
MspI CCGG 2 cut(s) 133, 219
MwoI GCNNNNNNNGC 2 cut(s) 228, 389
NdeII GATC 2 cut(s) 170, 676
NlaIII CATG 2 cut(s) 481, 545
NlaIV GGNNCC 4 cut(s) 122, 279, 650, 668
PaeR7I CTCGAG 1 cut(s) 17
PagI TCATGA 1 cut(s) 477
PciSI GCTCTTC 1 cut(s) 354
PfeI GAWTC 1 cut(s) 147
PinAI ACCGGT 1 cut(s) 132
PkrI GCNGC 3 cut(s) 112, 471, 516
PleI GAGTC 3 cut(s) 16, 415, 469
PpsI GAGTC 3 cut(s) 16, 415, 469
Psp124BI GAGCTC 1 cut(s) 54
Psp1406I AACGTT 1 cut(s) 418
PspN4I GGNNCC 4 cut(s) 122, 279, 650, 668
PspPI GGNCC 2 cut(s) 121, 152
RseI CAYNNNNRTG 1 cut(s) 552
Rsr2I CGGWCCG 1 cut(s) 152
RsrII CGGWCCG 1 cut(s) 152
SacI GAGCTC 1 cut(s) 54
SapI GCTCTTC 1 cut(s) 354
SaqAI TTAA 2 cut(s) 369, 413
SatI GCNGC 3 cut(s) 111, 470, 515
Sau3AI GATC 2 cut(s) 170, 676
Sau96I GGNCC 2 cut(s) 121, 152
SchI GAGTC 3 cut(s) 17, 416, 470
SduI GDGCHC 2 cut(s) 54, 247
SetI ASST 9 cut(s) 54, 321, 342, 382, 421, 445, 471, 616, 642
SfaNI GCATC 2 cut(s) 298, 441
SfcI CTRYAG 1 cut(s) 655
Sfr274I CTCGAG 1 cut(s) 17
SinI GGWCC 2 cut(s) 121, 152
SlaI CTCGAG 1 cut(s) 17
SmiMI CAYNNNNRTG 1 cut(s) 552
SmlI CTYRAG 2 cut(s) 17, 368
SmoI CTYRAG 2 cut(s) 17, 368
Sse9I AATT 2 cut(s) 75, 88
SsiI CCGC 3 cut(s) 5, 110, 155
SspMI CTAG 3 cut(s) 144, 161, 618
SstI GAGCTC 1 cut(s) 54
StyI CCWWGG 1 cut(s) 290
TaaI ACNGT 3 cut(s) 400, 526, 659
TaiI ACGT 1 cut(s) 421
TaqI TCGA 2 cut(s) 18, 48
TasI AATT 2 cut(s) 75, 88
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 1 cut(s) 147
Tru1I TTAA 2 cut(s) 369, 413
Tru9I TTAA 2 cut(s) 369, 413
TscAI CASTG 3 cut(s) 403, 529, 664
TseI GCWGC 2 cut(s) 469, 514
TspDTI ATGAA 3 cut(s) 197, 390, 565
TspGWI ACGGA 1 cut(s) 337
TspRI CASTG 3 cut(s) 403, 529, 664
Vha464I CTTAAG 1 cut(s) 368
VpaK11BI GGWCC 2 cut(s) 121, 152
XbaI TCTAGA 1 cut(s) 617
XhoI CTCGAG 1 cut(s) 17
XspI CTAG 3 cut(s) 144, 161, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.