Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004933

Physical Location & Seq

Genomic Coordinates Reverse (-)

231447 .. 233383

Gene Structure 1937 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1003500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 729 bp

ATGTCTGAAGTAAACTTATTTCTTCGTTACAGCTTTTGGACAAAGATTGCTGCAACTTCATCGAGGCAGTTATTCCTCTTAGGTCTAACTACAGTAAACCAGAGCCAAAAGTCGATTCACACTATTGTAGCAAACATGGCACAGCTCTTCAGTTACACGGCCTGCAGACAGTTGTCTCAGATGTTCCTGGCAGTAATATTTTTTCACGGTTCTGAATACATTTTAGCAGTTGGCATTCACGGGAAATCAAACGTTACTCTTAAGTCACTTCTGATCAGCAAAAACTATCTTCTTGCGATGGTTTTTTCCTTGGTGGAATACTGTGTTGAAATTATTTTGTTCCCTGGCCTCAAGGAACACCGGTGGGTCAGCAACTTAGGCCTTGCAATGGTCATAATAGGCGAAATCATACGGAAGATGGCAATAATTACAGCTGGTAAGTCCTTCACACATCTTATAAGGACTCATGCCTCAGAGCATCACAGATTGATTACGAATGGAGTTTATAGAGTTGTTCGTCATCCTGGTTACTGTGGTTTCTTCATATGGTCGGTGGGTACTCAGATAATGCTCTGTAATCCCGTATCAACAATTGCATTTGCACTTGTAGTTTGGCGCTTCTTTGCACAAAGAATTCCGTATGAAGAGTATTTCTTGAGGCAGTTTTTTGGGCCGCAATACGAGGAATATGGCCGACGGATTCCTTCGGGGGTGCCTTTTGTAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003880 GO:0004671 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006479 GO:0006481 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008213 GO:0008276 GO:0008757 GO:0009787 GO:0009788 GO:0009791 GO:0009908 GO:0009966 GO:0009968 GO:0009987 GO:0010340 GO:0010646 GO:0010648 GO:0012505 GO:0016740 GO:0016741 GO:0018410 GO:0019538 GO:0022414 GO:0023051 GO:0023057 GO:0032259 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048367 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051998 GO:0061458 GO:0065007 GO:0071704 GO:0090567 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

ko00900 ko01130 map00900 map01130

Pfam Domains

Protein Families

ICMT

Protein Analysis

243

Amino Acids

27.75

Weight (kDa)

9.78

Isoelectric Point (pI)

31.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PEMT	PF04191	126 - 225	2.4e-12	Phospholipid methyltransferase
ICMT	PF04140	128 - 219	9.7e-29	Isoprenylcysteine carboxyl methyltransferase (ICMT) family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004933)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08335 AT5G08335 AT5G23320 AT5G23320
malus_domestica	MD11G1238800.v1.1 MD13G1003500.v1.1 MD16G1003900.v1.1
prunus_persica	Prupe.1G348900_v2.0.a1 Prupe.1G348900_v2.0.a1
pyrus_communis	pycom13g00300 pycom16g00210
rosa_chinensis	RchiOBHm_Chr1g0318061 RchiOBHm_Chr1g0318091 RchiOBHm_Chr1g0318101 RchiOBHm_Chr4g0446911
rosa_laevigata	RLG00000005622 RLG00000030561 RLG00000030567
rosa_multiflora	Rmu_sc0001753.1_g000011 Rmu_sc0011424.1_g000010 Rmu_sc0011539.1_g000006
rosa_roxburghii	Rroxscaffold_4G00329550 Rroxscaffold_4G00329600
rosa_rugosa	Rorug04G0375900
rosa_samantha	Rh4AG437100 Rh4BG446300 Rh4CG463600 Rh4DG444900
rosa_wichuraiana	Rw1G002410 Rw4G037400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	458
AccB1I	GGYRCC	1 cut(s)	712
AciI	CCGC	1 cut(s)	674
AclI	AACGTT	1 cut(s)	252
AcoI	YGGCCR	1 cut(s)	691
AcsI	RAATTY	1 cut(s)	633
AcuI	CTGAAG	2 cut(s)	27, 133
AfaI	GTAC	1 cut(s)	559
AfiI	CCNNNNNNNGG	1 cut(s)	388
AflII	CTTAAG	1 cut(s)	260
AgeI	ACCGGT	1 cut(s)	360
AgsI	TTSAA	1 cut(s)	329
AjnI	CCWGG	3 cut(s)	186, 343, 523
AjuI	GAANNNNNNNTTGG	2 cut(s)	99, 131
AluBI	AGCT	3 cut(s)	33, 145, 434
AluI	AGCT	3 cut(s)	33, 145, 434
Alw26I	GTCTC	1 cut(s)	180
AoxI	GGCC	5 cut(s)	159, 346, 379, 671, 691
ApeKI	GCWGC	1 cut(s)	50
ApoI	RAATTY	1 cut(s)	633
AsiGI	ACCGGT	1 cut(s)	360
AspLEI	GCGC	1 cut(s)	618
AspS9I	GGNCC	1 cut(s)	671
BanI	GGYRCC	1 cut(s)	712
BbvI	GCAGC	1 cut(s)	37
BccI	CCATC	2 cut(s)	292, 412
BceAI	ACGGC	1 cut(s)	174
BcgI	CGANNNNNNTGC	2 cut(s)	42, 76
BciT130I	CCWGG	3 cut(s)	188, 345, 525
BclI	TGATCA	1 cut(s)	273
BcoDI	GTCTC	1 cut(s)	180
BfmI	CTRYAG	2 cut(s)	90, 163
BfoI	RGCGCY	1 cut(s)	619
BfrI	CTTAAG	1 cut(s)	260
BisI	GCNGC	2 cut(s)	51, 674
BlsI	GCNGC	2 cut(s)	52, 675
Bme1390I	CCNGG	3 cut(s)	188, 345, 525
BmgT120I	GGNCC	1 cut(s)	671
BmiI	GGNNCC	1 cut(s)	714
BmrFI	CCNGG	3 cut(s)	188, 345, 525
BmsI	GCATC	1 cut(s)	487
BoxI	GACNNNNGTC	1 cut(s)	172
BpuEI	CTTGAG	2 cut(s)	335, 676
BsaJI	CCNNGG	2 cut(s)	309, 343
BsaWI	WCCGGW	1 cut(s)	360
Bsc4I	CCNNNNNNNGG	1 cut(s)	388
Bse118I	RCCGGY	1 cut(s)	360
Bse3DI	GCAATG	1 cut(s)	393
BseBI	CCWGG	3 cut(s)	188, 345, 525
BseDI	CCNNGG	2 cut(s)	309, 343
BseGI	GGATG	1 cut(s)	520
BseLI	CCNNNNNNNGG	1 cut(s)	388
BseMI	GCAATG	1 cut(s)	393
BseMII	CTCAG	3 cut(s)	191, 486, 575
BseXI	GCAGC	1 cut(s)	37
BshFI	GGCC	5 cut(s)	161, 348, 381, 673, 693
BshNI	GGYRCC	1 cut(s)	712
BshTI	ACCGGT	1 cut(s)	360
BsiSI	CCGG	1 cut(s)	361
BslI	CCNNNNNNNGG	1 cut(s)	388
BsmAI	GTCTC	1 cut(s)	180
BsmI	GAATGC	1 cut(s)	234
BsnI	GGCC	5 cut(s)	161, 348, 381, 673, 693
Bsp143I	GATC	1 cut(s)	273
BspACI	CCGC	1 cut(s)	674
BspANI	GGCC	5 cut(s)	161, 348, 381, 673, 693
BspCNI	CTCAG	3 cut(s)	190, 485, 574
BspLI	GGNNCC	1 cut(s)	714
BspMAI	CTGCAG	1 cut(s)	167
BspQI	GCTCTTC	1 cut(s)	152
BspT107I	GGYRCC	1 cut(s)	712
BspTI	CTTAAG	1 cut(s)	260
BsrDI	GCAATG	1 cut(s)	393
BsrFI	RCCGGY	1 cut(s)	360
BssAI	RCCGGY	1 cut(s)	360
BssECI	CCNNGG	2 cut(s)	309, 343
BssMI	GATC	1 cut(s)	273
BssT1I	CCWWGG	1 cut(s)	309
Bst2UI	CCWGG	3 cut(s)	188, 345, 525
Bst4CI	ACNGT	5 cut(s)	94, 171, 209, 323, 533
Bst6I	CTCTTC	2 cut(s)	152, 639
BstAFI	CTTAAG	1 cut(s)	260
BstC8I	GCNNGC	1 cut(s)	163
BstDEI	CTNAG	5 cut(s)	79, 177, 376, 472, 561
BstF5I	GGATG	1 cut(s)	520
BstH2I	RGCGCY	1 cut(s)	619
BstHHI	GCGC	1 cut(s)	618
BstKTI	GATC	1 cut(s)	276
BstMAI	GTCTC	1 cut(s)	180
BstMBI	GATC	1 cut(s)	273
BstMWI	GCNNNNNNNGC	2 cut(s)	137, 378
BstNI	CCWGG	3 cut(s)	188, 345, 525
BstPAI	GACNNNNGTC	1 cut(s)	172
BstSCI	CCNGG	3 cut(s)	186, 343, 523
BstSFI	CTRYAG	2 cut(s)	90, 163
BstV1I	GCAGC	1 cut(s)	37
BsuRI	GGCC	5 cut(s)	161, 348, 381, 673, 693
BtgZI	GCGATG	1 cut(s)	311
BtsCI	GGATG	1 cut(s)	520
Cac8I	GCNNGC	1 cut(s)	163
CfoI	GCGC	1 cut(s)	618
Cfr10I	RCCGGY	1 cut(s)	360
Cfr13I	GGNCC	1 cut(s)	671
Csp6I	GTAC	1 cut(s)	558
CspAI	ACCGGT	1 cut(s)	360
CviAII	CATG	2 cut(s)	136, 467
CviJI	RGCY	9 cut(s)	33, 105, 145, 161, 348, 381, 434, 673, 693
CviKI_1	RGCY	9 cut(s)	33, 105, 145, 161, 348, 381, 434, 673, 693
CviQI	GTAC	1 cut(s)	558
DdeI	CTNAG	5 cut(s)	79, 177, 376, 472, 561
DpnI	GATC	1 cut(s)	275
DpnII	GATC	1 cut(s)	273
EaeI	YGGCCR	1 cut(s)	691
Eam1104I	CTCTTC	2 cut(s)	152, 639
EarI	CTCTTC	2 cut(s)	152, 639
Eco130I	CCWWGG	1 cut(s)	309
Eco147I	AGGCCT	1 cut(s)	381
Eco57I	CTGAAG	2 cut(s)	27, 133
EcoRI	GAATTC	1 cut(s)	633
EcoRII	CCWGG	3 cut(s)	186, 343, 523
EcoT14I	CCWWGG	1 cut(s)	309
ErhI	CCWWGG	1 cut(s)	309
FaeI	CATG	2 cut(s)	139, 470
FatI	CATG	2 cut(s)	135, 466
FauNDI	CATATG	1 cut(s)	545
FbaI	TGATCA	1 cut(s)	273
Fnu4HI	GCNGC	2 cut(s)	51, 674
FokI	GGATG	1 cut(s)	507
Fsp4HI	GCNGC	2 cut(s)	51, 674
GlaI	GCGC	1 cut(s)	617
GluI	GCNGC	2 cut(s)	51, 674
HaeII	RGCGCY	1 cut(s)	619
HaeIII	GGCC	5 cut(s)	161, 348, 381, 673, 693
HapII	CCGG	1 cut(s)	361
HhaI	GCGC	1 cut(s)	618
Hin1II	CATG	2 cut(s)	139, 470
Hin6I	GCGC	1 cut(s)	616
HinP1I	GCGC	1 cut(s)	616
HinfI	GANTC	3 cut(s)	115, 463, 700
HpaII	CCGG	1 cut(s)	361
Hpy166II	GTNNAC	2 cut(s)	13, 97
Hpy188I	TCNGA	6 cut(s)	7, 180, 214, 273, 475, 564
Hpy188III	TCNNGA	1 cut(s)	655
Hpy8I	GTNNAC	2 cut(s)	13, 97
Hpy99I	CGWCG	1 cut(s)	699
HpyAV	CCTTC	2 cut(s)	454, 714
HpyCH4III	ACNGT	5 cut(s)	94, 171, 209, 323, 533
HpyCH4IV	ACGT	1 cut(s)	252
HpyCH4V	TGCA	6 cut(s)	53, 165, 386, 596, 602, 626
HpyF10VI	GCNNNNNNNGC	2 cut(s)	137, 378
HpyF3I	CTNAG	5 cut(s)	79, 177, 376, 472, 561
HpySE526I	ACGT	1 cut(s)	252
Hsp92II	CATG	2 cut(s)	139, 470
HspAI	GCGC	1 cut(s)	616
Ksp22I	TGATCA	1 cut(s)	273
Kzo9I	GATC	1 cut(s)	273
LguI	GCTCTTC	1 cut(s)	152
Lsp1109I	GCAGC	1 cut(s)	37
LweI	GCATC	1 cut(s)	487
MaeII	ACGT	1 cut(s)	252
MaeIII	GTNAC	5 cut(s)	26, 152, 253, 264, 527
MalI	GATC	1 cut(s)	275
MboI	GATC	1 cut(s)	273
MboII	GAAGA	6 cut(s)	14, 139, 281, 427, 532, 656
MfeI	CAATTG	1 cut(s)	591
MluCI	AATT	4 cut(s)	330, 426, 591, 633
MlyI	GAGTC	1 cut(s)	457
MnlI	CCTC	6 cut(s)	57, 86, 359, 481, 651, 676
MseI	TTAA	1 cut(s)	261
MspA1I	CMGCKG	1 cut(s)	434
MspCI	CTTAAG	1 cut(s)	260
MspI	CCGG	1 cut(s)	361
MspR9I	CCNGG	3 cut(s)	188, 345, 525
MunI	CAATTG	1 cut(s)	591
Mva1269I	GAATGC	1 cut(s)	234
MvaI	CCWGG	3 cut(s)	188, 345, 525
MwoI	GCNNNNNNNGC	2 cut(s)	137, 378
NdeI	CATATG	1 cut(s)	545
NdeII	GATC	1 cut(s)	273
NlaIII	CATG	2 cut(s)	139, 470
NlaIV	GGNNCC	1 cut(s)	714
NmuCI	GTSAC	1 cut(s)	264
PceI	AGGCCT	1 cut(s)	381
PciSI	GCTCTTC	1 cut(s)	152
PctI	GAATGC	1 cut(s)	234
PfeI	GAWTC	2 cut(s)	115, 700
PinAI	ACCGGT	1 cut(s)	360
PkrI	GCNGC	2 cut(s)	52, 675
PleI	GAGTC	1 cut(s)	457
PpsI	GAGTC	1 cut(s)	457
PshAI	GACNNNNGTC	1 cut(s)	172
PsiI	TTATAA	1 cut(s)	458
Psp1406I	AACGTT	1 cut(s)	252
Psp6I	CCWGG	3 cut(s)	186, 343, 523
PspGI	CCWGG	3 cut(s)	186, 343, 523
PspN4I	GGNNCC	1 cut(s)	714
PspPI	GGNCC	1 cut(s)	671
PstI	CTGCAG	1 cut(s)	167
PvuII	CAGCTG	1 cut(s)	434
RsaI	GTAC	1 cut(s)	559
RsaNI	GTAC	1 cut(s)	558
SapI	GCTCTTC	1 cut(s)	152
SaqAI	TTAA	1 cut(s)	261
SatI	GCNGC	2 cut(s)	51, 674
Sau3AI	GATC	1 cut(s)	273
Sau96I	GGNCC	1 cut(s)	671
SchI	GAGTC	1 cut(s)	457
ScrFI	CCNGG	3 cut(s)	188, 345, 525
SetI	ASST	5 cut(s)	35, 85, 147, 255, 436
SfaNI	GCATC	1 cut(s)	487
SfcI	CTRYAG	2 cut(s)	90, 163
SgrAI	CRCCGGYG	1 cut(s)	360
SmlI	CTYRAG	3 cut(s)	260, 350, 655
SmoI	CTYRAG	3 cut(s)	260, 350, 655
Sse9I	AATT	4 cut(s)	330, 426, 591, 633
SseBI	AGGCCT	1 cut(s)	381
SsiI	CCGC	1 cut(s)	674
SspI	AATATT	1 cut(s)	198
StuI	AGGCCT	1 cut(s)	381
StyD4I	CCNGG	3 cut(s)	186, 343, 523
StyI	CCWWGG	1 cut(s)	309
TaaI	ACNGT	5 cut(s)	94, 171, 209, 323, 533
TaiI	ACGT	1 cut(s)	255
TaqI	TCGA	2 cut(s)	62, 113
TasI	AATT	4 cut(s)	330, 426, 591, 633
TauI	GCSGC	1 cut(s)	676
TfiI	GAWTC	2 cut(s)	115, 700
Tru1I	TTAA	1 cut(s)	261
Tru9I	TTAA	1 cut(s)	261
TseFI	GTSAC	1 cut(s)	264
TseI	GCWGC	1 cut(s)	50
Tsp45I	GTSAC	1 cut(s)	264
TspDTI	ATGAA	3 cut(s)	48, 532, 657
TspGWI	ACGGA	3 cut(s)	427, 627, 712
Vha464I	CTTAAG	1 cut(s)	260
XapI	RAATTY	1 cut(s)	633

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.