Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0004933

Physical Location & Seq

Genomic Coordinates Forward (+)

54654894 .. 54668786

Gene Structure 13893 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0375900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5100 bp

ATGCTACTACTCACAAACTCTCTCTCTTTCCTACACTCCAACACAAGGTCTCTCCCTCACACCAAACCCTGGCCGCCGCCGCCGCCGCCTTTCCCGTTCCTCCACCCAAACTCCCGCCATCTCCGCCTCCCTCCTAACTCCAAGGTCGTTCGAGGCGTGAGATTCGACGGCCCGGCCATCGATATCAGCCAAGTGACGGACACCGACGACGGCGATTTGATAATCGAGACTTGCCTCACGCGGAACTTGACTCCGGCGTTGTCTATTGAGCAAGGGCTTCAGAGGATCAAGGAGGCGGTTGAGGAGTTGAAGCTCAATCCTCCTTCAACTTGTAGTGGAATTCTCAGGTTTCAGGTGGCTGTGCCGCCGAGTGCGAAAGCCTTGAACTGGTTTTGCTGCCAACCGGAGTCGTCTGTGGTCTATCCACTGTTTTTTGTTTCCAAGGAAACTGAAAATCCGAGCTATAAATCACTGTATGTGAATGAAACTCGAGGAGTTTTCGGAATCGGTGCTGCAATTTACTTCACACCTTCTTCTAATTCTTCTTCTTCGATCAAAAGAATTCTATCAAATGAATCAGCTTTTGTAATAGCTTATGGTTTTATGGATATTAGTTACGACCAAGAGTCATCTTATATGAAGCATGAGGCAGGTTCATATTACTTTTTTATTCCTCAGATTGAGTTACATGAGCATGAGGGCGCTTCTGTATTAGCAGCAACTATTGGTTGGAGTGAGTCTTCCCCTTGTACATTTGAGGAAGCTGTTCTTTCTTATGAGTTATGTTTCAACCAGGTTACCTGTCAAGTTTGTTCCACAACAAAGAAATCCCTCACCACAAATATAAGATCTACTCTTAGGAAGCTCAATCTTCTGGAGGATGGAATGGTTTCTATGGTACACATGAACATGCTGTTAGCAGGTGGAAAAAACATTTCGTCTGACATCATGGCACTGAAAGAGGCTCCGTCTTCCTCCCAGTTTTGTGTGAGGCTTTCACCGACCATGGCTGTTGCTAATAAAATGTTGGATCATGCCAGTAAAATGTGCTTCTCAGTGCAAGATAGTGGCAACATCAACACTGTTTGGGCATCACTTATAGTTGAAGAATGTTCTCGACTTGGTTTGACGTATTTTTGTGTAGCTCCAGGATCAAGATCATCCCCCCTTGCTGTTGCTGCATCCACTCATCCACTTATTACATGTATTGTATGTTATGATGAACGCTCACTTGCATTTCATGCTGTTGGGTATGCAAGAGGATCTGGCAAACCAGCAGTAGTCATAACATCATCTGGCACTGCAGTTTCAAATCTTCTTCCTGCTGTGGTCGAGGCTAGTCAAGATTTTGTACCACTTATATTGCTCACAGCTGATCGTCCTGCAGAACTGCAGGATGCTGGAGCAAACCAAGCAATCAATCAGGTAAACCATTTTGGCTCTTTTGCACGGTTTTTCTTTAGCCTTCCTGCACCCACAGATCATATTTCAGCTCGCATGGTGCTTACAACACTCGACAATGCTGTACATTGGGCAACCTCTTCACCATGTGGGCCTGCTCATATTAACTGTCCTTTCAGGGAGCCACTAGAAGATAGCCCTAGAAAATGGATGTTGAGTTGTTTGAATGGATTAAACCATTGGATGTCTAATTCTGAACCATTTACCAAGTATATTCAAGTACAACATGCTCATACAAGTAATGATGGTTGTGGCGGAATGTCAGAAATTCTAAATGTAATTAAGGGAACTAACAAGGGTCTTCTACTTATCGGTGCAATCCATTCAGAAGATGAGATGTGGGCAGTTCTTCTCTTGGCTAAACACCTTCACTGGCCTGTTATAGCTGACATCTTGTCAGGATTACGATTGAGAAAGCTTTTGACTAGTTTCCCTGAAGTTGACAATGATTTATTGTTTGTTGATCATCTTGACCATGCCTTACTGTCAGATTCTGTCAGTAAATGGATTAACATTGATGTGATTATTCAGATTGGGAGCAGGATTACAAGCAAGCGTATTGCTAAGATGCTAGAGGAATCCTTTCCCTGCTCATATATCATGGTTGACAAGCATCCATTTCGTCACGATCCTTCTCATATTGTAACTCATAGAGTCCACAGCAACATTTTTGAATTTGCTGATTATATCCTTAAAGCTGAATTTCCCCATCTCAGCAAGGAATGGAGCACTTATCTTCAAATGATAAATGCAATGGTTGAAAGGGAGTTGTCATTTCAAATTTCTGCTAGACACTCCTTGACAGAACCTCAAGTCGCATACCTAATTTCAGAAGCACTTTCAGCTGAATCTGCTCTTTTCATTGGGAACAGTATGGCAATACGTGATGCAGATATGTATGGGCGTGGTTGGTCAGAGTATACACGTAGTATTGTTGCCATGAATTATAAATTACCCTGTCAAATGATTCGGGTGGCTGGGAATAGAGGTGCAAGTGGTATTGATGGGTTACTCAGTACAGCTGTTGGCTTTGCTATAGGATGCAATAAACGAGTACTTTGCGTGATTGGAGATGTTTCCTTCCTCCATGATACAAATGGCTTAGCAATTGTGAACCAGAGGACGTTCCGGAAACCAATGACGATAGTTGTGATTAACAATCATGGTGGTGCAATATTCAGTCTCCTTCCTCTTGCAGATAGAGTCAAACCAAGAATACTAAACCAATATTTCTACACCTCTCACAACATATCAATCCGTGAGCTTTGTGTAGCACATGGTGTGATGCATCTACATGCGAAGACAAAGTTGGATTTTGAAGATGCATTATTCACATCCCAACATGGAGGGATGGATTGTGTCATTGAAGTTGAGAGCTGCATAGATACCAATGCCAGCTTTCACAGTACTTTAAAAACATTTGCATGCCAAGCAGCAGATCAAGTCATAAAGCCTTCTAGTCAAGACTCGATATTAGATGACACTTTACTCTGCAGAGTCCATAGAATGGAGTACTCATTGTTTAGAATGCCACTGTGTGCTCCTCATACTATGGTTTCTGTTGATGGCGGTACCACCAGCTTCCACAGGGAAGGGTTTATTTTAACTTTGTATTTTGAAGATGGAAGCTTTGGGCTTGGCGAGGTTTCACCCCTAGACATCTACAAAGAAAACCTTCTAGATGTAGAAGAGCAACTGAGGTTTCTCATCCATATGATGAAAGGAGCTCATATCAGTTGCTTCCTTCCTCTGCTAAAGGGCTCGTTTTCATCTTGGATATGGTCCAACCTTGGAATCCTGCCATGTACGCTCTTTCCTAGTGTGAGATGTGGTTTGGAAATGGCAATTCTCAATGCCATAGCAAATAGACAAGGTTCCAATTTATTAGGAATACTCCATCCCCAGATAGTTGGAGATATATTTAAATCGTCATCAACAGTTCAGATTTGTGCCCTTGTTGATTCCAGTGGGACACCCACACAAGTAGCTGATGCTATTGCTACACTTGTTGAAGAAGGATTTACTGCAGTAAAGATTAAGGTAGCTCGTTGTGGGAGTCCCTTACAGGATGCTGCAGTTATACAAGAAGTAAGAAAAAAAGTTGGATACCATATTAAAATTCGTGTAGATGTCAATCGGAACTGGACCTATGAAGAAGCTATCCAGTTTGGTTCCTTGGTAAAGGATTGTGATCTGCAGTATATCGAGGAACCTGTTCAAATTGAAGAAGATATTATCAAGTTTTGCAAAGAAAGTGGGTTGCCTGTGGCACTGGATGAAACTATTGACAATATTGGAGAACATCCACTTATAAAGCTCTCAAAGTATGCCCATCCAGGAATAGTAGCTGTTGTCATCAAGCCAAGTGTTGTTGGTGGCTTTGAAAATGCAGCAATAATTGCCCAATGGGCCCAGCAGCACCAGAAAATGGCTGTTGTTAGTGCTGCATTTGAAAGCAGCCTAGGTTTGTCAGCATATATTCAGTTTTCCTGTTACCTCAACCAGATGAATTCAGAGATTTGTACAATGATGAACTATCCATTAGCCTCATCCATAGCCCATGGTTTAGGAACTTACCGCTGGCTAAAAGAAGATTTGACGACTACTCCTCTCAAGATAAATCGTAATCCACGTAGTGGGTTCGTAGAAGCATCTGTTGCTGATTCTGATCGAGTTCTGCAGCAATTTCAAATCAATGGAAACATAATTCGTAGAAAGTTTACTGGAGAGCAAGTTTGTATATACCAGTTGCCTTTAGATTCAAAGGGTTTATCTTGCTCCATCAAAATTCAGGAGATTGGACAAGGATATGATGATAATGTATTTGTATTTCTTCATGGGTTTCTTGGAACTGGTGAAGATTGGATTGCTATCATGAAGGCCTTATCAGGATGTGGAAGATGCGTTTCTATTGACCTTCCTGGTCATGGTGGAACCAAGATACAAAGTCATGGTGATAAGGATGCTGTACAGGCTTCAGGTTTGTGTGTTGAGGTAGTTGCCGATCTTTTATGTAAAGTGATTGAGCATATTACTCCTGGGAAGGTTACCCTTGTCGGATATTCTATGGGAGCCAGGATCGCCCTTTGCATGGCTCTGAAGCTTACTAAAAAAGTTAAGGGAGCTATTATCATATCTGGCAGCCCTGGAATAAATGATGGAATGGCAAGAAAAATTCGTAGAGCTGAAGATGACTCTAGAGCACGCTTCCTTGCTGCATATGGCTTAAAGCACTTTCTAGACACCTGGTATGCTGGAGAGCTTTGGAACAGCTTAAGGGAACACCCTCACTTTCATCAGATAGCCACCGACCGCCTGCATCATGGAGATGTGCAGAGTCTTGCAAAAGTTCTGTCTGCTTTAAGTGTTGGGAGGCAGCTGCCATTGTGGGAAGACTTGAAGCACTGCAAAACGCCTCTTTTGCTCATTGTTGGAGAGAAAGATAAAAAATTCAAAACAATAGCTCAGGATATGTTCCTTGCGATTGGTAGTGGCAATGGCAAATTGACTGGAGATGATGGCGCACCCAACGACCTTTGTGAGATTGTTGAGATCCCAGATTGTGGACATGCTGCTCATCTGGAAAACCCTCTCCCTGTAATCAGGGCATTAAGGCAATTTGTGACGAAACTGAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003880 GO:0004671 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006464 GO:0006479 GO:0006481 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008213 GO:0008276 GO:0008757 GO:0009787 GO:0009788 GO:0009791 GO:0009908 GO:0009966 GO:0009968 GO:0009987 GO:0010340 GO:0010646 GO:0010648 GO:0012505 GO:0016740 GO:0016741 GO:0018410 GO:0019538 GO:0022414 GO:0023051 GO:0023057 GO:0032259 GO:0032501 GO:0032502 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048367 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051998 GO:0061458 GO:0065007 GO:0071704 GO:0090567 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

ko00900 ko01130 map00900 map01130

Pfam Domains

Protein Families

ICMT

Protein Analysis

1699

Amino Acids

187.19

Weight (kDa)

6.31

Isoelectric Point (pI)

39.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	364 - 476	3.6e-22	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M_2	PF16582	542 - 775	2.8e-23	Middle domain of thiamine pyrophosphate
TPP_enzyme_C	PF02775	825 - 934	2.3e-06	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain
MR_MLE_C	PF13378	1149 - 1324	4.8e-24	Enolase C-terminal domain-like
Abhydrolase_1	PF00561	1427 - 1683	3.9e-19	alpha/beta hydrolase fold
Hydrolase_4	PF12146	1427 - 1646	1.9e-11	Serine aminopeptidase, S33
Abhydrolase_6	PF12697	1429 - 1689	4.8e-22	Alpha/beta hydrolase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004933)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G08335 AT5G08335 AT5G23320 AT5G23320
malus_domestica	MD11G1238800.v1.1 MD13G1003500.v1.1 MD16G1003900.v1.1
prunus_persica	Prupe.1G348900_v2.0.a1 Prupe.1G348900_v2.0.a1
pyrus_communis	pycom13g00300 pycom16g00210
rosa_chinensis	RchiOBHm_Chr1g0318061 RchiOBHm_Chr1g0318091 RchiOBHm_Chr1g0318101 RchiOBHm_Chr4g0446911
rosa_laevigata	RLG00000005622 RLG00000030561 RLG00000030567
rosa_multiflora	Rmu_sc0001753.1_g000011 Rmu_sc0011424.1_g000010 Rmu_sc0011539.1_g000006
rosa_roxburghii	Rroxscaffold_4G00329550 Rroxscaffold_4G00329600
rosa_rugosa	Rorug04G0375900
rosa_samantha	Rh4AG437100 Rh4BG446300 Rh4CG463600 Rh4DG444900
rosa_wichuraiana	Rw1G002410 Rw4G037400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2409, 3773
AarI	CACCTGC	1 cut(s)	911
Acc36I	ACCTGC	2 cut(s)	641, 911
Acc65I	GGTACC	1 cut(s)	3032
AccB1I	GGYRCC	1 cut(s)	3032
AccB7I	CCANNNNNTGG	5 cut(s)	69, 2968, 3889, 4097, 5024
AccI	GTMKAC	1 cut(s)	2381
AccII	CGCG	1 cut(s)	241
AccIII	TCCGGA	1 cut(s)	2589
AclWI	GGATC	7 cut(s)	293, 1038, 1159, 1270, 2084, 4547, 5008
AcoI	YGGCCR	2 cut(s)	71, 174
AcuI	CTGAAG	5 cut(s)	263, 1917, 4422, 4580, 4668
AdeI	CACNNNGTG	4 cut(s)	1550, 2741, 2999, 4097
AflII	CTTAAG	1 cut(s)	4735
AflIII	ACRYGT	2 cut(s)	1202, 2384
AhdI	GACNNNNNGTC	2 cut(s)	625, 1855
AhlI	ACTAGT	1 cut(s)	1886
AjnI	CCWGG	9 cut(s)	68, 792, 1147, 3796, 4381, 4498, 4535, 4606, 4706
AjuI	GAANNNNNNNTTGG	2 cut(s)	2753, 2785
Alw21I	GWGCWC	4 cut(s)	2192, 3004, 3190, 4666
Alw26I	GTCTC	3 cut(s)	54, 221, 2648
AlwI	GGATC	7 cut(s)	293, 1038, 1159, 1270, 2084, 4547, 5008
AlwNI	CAGNNNCTG	1 cut(s)	1955
Ama87I	CYCGRG	1 cut(s)	489
Aor13HI	TCCGGA	1 cut(s)	2589
AoxI	GGCC	7 cut(s)	71, 169, 174, 1553, 1835, 3870, 4341
ApaI	GGGCCC	1 cut(s)	3874
ArsI	GACNNNNNNTTYG	2 cut(s)	4799, 4831
Asp700I	GAANNNNTTC	5 cut(s)	765, 889, 2043, 3135, 3675
Asp718I	GGTACC	1 cut(s)	3032
AspA2I	CCTAGG	1 cut(s)	3922
AspLEI	GCGC	2 cut(s)	704, 4985
AspS9I	GGNCC	6 cut(s)	170, 1553, 3243, 3606, 3870, 3871
AsuC2I	CCSGG	1 cut(s)	173
AsuHPI	GGTGA	6 cut(s)	826, 990, 1536, 3102, 4328, 4427
AvaI	CYCGRG	1 cut(s)	489
AvaII	GGWCC	2 cut(s)	3243, 3606
AvrII	CCTAGG	1 cut(s)	3922
BaeGI	GKGCMC	2 cut(s)	3415, 3874
BanI	GGYRCC	1 cut(s)	3032
BanII	GRGCYC	3 cut(s)	3190, 3224, 3874
BbsI	GAAGAC	5 cut(s)	732, 963, 1754, 2768, 4860
Bbv12I	GWGCWC	4 cut(s)	2192, 3004, 3190, 4666
BceAI	ACGGC	2 cut(s)	184, 226
BcgI	CGANNNNNNTGC	4 cut(s)	2063, 2097, 2502, 2536
BciT130I	CCWGG	9 cut(s)	70, 794, 1149, 3798, 4383, 4500, 4537, 4608, 4708
BciVI	GTATCC	1 cut(s)	3560
BclI	TGATCA	1 cut(s)	1924
BcnI	CCSGG	1 cut(s)	173
BcoDI	GTCTC	3 cut(s)	54, 221, 2648
BcuI	ACTAGT	1 cut(s)	1886
BfmI	CTRYAG	9 cut(s)	1302, 1383, 1391, 2496, 2953, 3486, 3534, 3656, 4139
BfoI	RGCGCY	1 cut(s)	705
BfrI	CTTAAG	1 cut(s)	4735
BfuAI	ACCTGC	2 cut(s)	641, 911
BfuI	GTATCC	1 cut(s)	3560
BglI	GCCNNNNNGGC	1 cut(s)	3869
BglII	AGATCT	1 cut(s)	848
BlnI	CCTAGG	1 cut(s)	3922
BlpI	GCTNAGC	1 cut(s)	2563
BmcAI	AGTACT	3 cut(s)	2517, 2869, 2975
Bme18I	GGWCC	2 cut(s)	3243, 3606
BmeRI	GACNNNNNGTC	2 cut(s)	625, 1855
BmeT110I	CYCGRG	1 cut(s)	489
BmgT120I	GGNCC	6 cut(s)	170, 1553, 3243, 3606, 3870, 3871
BmiI	GGNNCC	9 cut(s)	966, 1584, 3034, 3337, 3634, 3672, 3872, 4396, 4534
BmrI	ACTGGG	1 cut(s)	973
BmuI	ACTGGG	1 cut(s)	973
BpiI	GAAGAC	5 cut(s)	732, 963, 1754, 2768, 4860
BpmI	CTGGAG	6 cut(s)	896, 1131, 1422, 4206, 4737, 4992
Bpu10I	CCTNAGC	1 cut(s)	4926
Bpu1102I	GCTNAGC	1 cut(s)	2563
BpuEI	CTTGAG	2 cut(s)	2256, 4058
BpuMI	CCSGG	1 cut(s)	173
Bsa29I	ATCGAT	1 cut(s)	180
BsaAI	YACGTR	3 cut(s)	2345, 2387, 4094
BsaBI	GATNNNNATC	5 cut(s)	1156, 3594, 3651, 4128, 4331
BsaI	GGTCTC	1 cut(s)	54
BsaWI	WCCGGW	2 cut(s)	403, 2589
BsaXI	ACNNNNNCTCC	6 cut(s)	95, 125, 4051, 4081, 4179, 4209
Bse3DI	GCAATG	2 cut(s)	2220, 4963
Bse8I	GATNNNNATC	5 cut(s)	1156, 3594, 3651, 4128, 4331
BseAI	TCCGGA	1 cut(s)	2589
BseBI	CCWGG	9 cut(s)	70, 794, 1149, 3798, 4383, 4500, 4537, 4608, 4708
BseCI	ATCGAT	1 cut(s)	180
BseJI	GATNNNNATC	5 cut(s)	1156, 3594, 3651, 4128, 4331
BseMI	GCAATG	2 cut(s)	2220, 4963
BseMII	CTCAG	7 cut(s)	358, 689, 1068, 2188, 2488, 3149, 4940
BseRI	GAGGAG	4 cut(s)	317, 507, 2994, 4059
BseSI	GKGCMC	2 cut(s)	3415, 3874
BseYI	CCCAGC	2 cut(s)	2438, 3873
BsgI	GTGCAG	2 cut(s)	1455, 4814
Bsh1236I	CGCG	1 cut(s)	241
Bsh1285I	CGRYCG	1 cut(s)	4774
BshFI	GGCC	7 cut(s)	73, 171, 176, 1555, 1837, 3872, 4343
BshNI	GGYRCC	1 cut(s)	3032
BshVI	ATCGAT	1 cut(s)	180
BsiEI	CGRYCG	1 cut(s)	4774
BsiHKAI	GWGCWC	4 cut(s)	2192, 3004, 3190, 4666
BsiHKCI	CYCGRG	1 cut(s)	489
BsiSI	CCGG	4 cut(s)	173, 254, 404, 2590
BslFI	GGGAC	2 cut(s)	3445, 3504
BsmAI	GTCTC	3 cut(s)	54, 221, 2648
BsmFI	GGGAC	2 cut(s)	3445, 3504
BsmI	GAATGC	1 cut(s)	2994
BsnI	GGCC	7 cut(s)	73, 171, 176, 1555, 1837, 3872, 4343
Bso31I	GGTCTC	1 cut(s)	54
BsoBI	CYCGRG	1 cut(s)	489
Bsp120I	GGGCCC	1 cut(s)	3870
Bsp1286I	GDGCHC	7 cut(s)	2192, 3004, 3190, 3224, 3415, 3874, 4666
Bsp13I	TCCGGA	1 cut(s)	2589
Bsp1407I	TGTACA	4 cut(s)	749, 1525, 3983, 4429
Bsp1720I	GCTNAGC	1 cut(s)	2563
Bsp19I	CCATGG	2 cut(s)	1005, 4021
BspANI	GGCC	7 cut(s)	73, 171, 176, 1555, 1837, 3872, 4343
BspCNI	CTCAG	7 cut(s)	357, 688, 1067, 2187, 2487, 3150, 4939
BspDI	ATCGAT	1 cut(s)	180
BspEI	TCCGGA	1 cut(s)	2589
BspFNI	CGCG	1 cut(s)	241
BspHI	TCATGA	1 cut(s)	4335
BspLI	GGNNCC	9 cut(s)	966, 1584, 3034, 3337, 3634, 3672, 3872, 4396, 4534
BspMAI	CTGCAG	8 cut(s)	1306, 1387, 1395, 2957, 3490, 3538, 3660, 4143
BspMI	ACCTGC	2 cut(s)	641, 911
BspPI	GGATC	7 cut(s)	293, 1038, 1159, 1270, 2084, 4547, 5008
BspQI	GCTCTTC	1 cut(s)	3144
BspT107I	GGYRCC	1 cut(s)	3032
BspTI	CTTAAG	1 cut(s)	4735
BspTNI	GGTCTC	1 cut(s)	54
BsrDI	GCAATG	2 cut(s)	2220, 4963
BsrGI	TGTACA	4 cut(s)	749, 1525, 3983, 4429
BssNAI	GTATAC	1 cut(s)	2382
BssT1I	CCWWGG	7 cut(s)	141, 441, 1005, 3250, 3636, 3922, 4021
Bst1107I	GTATAC	1 cut(s)	2382
Bst2UI	CCWGG	9 cut(s)	70, 794, 1149, 3798, 4383, 4500, 4537, 4608, 4708
Bst6I	CTCTTC	2 cut(s)	1546, 3144
BstAFI	CTTAAG	1 cut(s)	4735
BstAPI	GCANNNNNTGC	3 cut(s)	1241, 3860, 4118
BstAUI	TGTACA	4 cut(s)	749, 1525, 3983, 4429
BstBAI	YACGTR	3 cut(s)	2345, 2387, 4094
BstC8I	GCNNGC	8 cut(s)	1495, 1557, 2015, 2857, 2887, 4043, 4666, 4778
BstDSI	CCRYGG	2 cut(s)	1005, 4021
BstEII	GGTNACC	2 cut(s)	796, 4507
BstFNI	CGCG	1 cut(s)	241
BstH2I	RGCGCY	1 cut(s)	705
BstHHI	GCGC	2 cut(s)	704, 4985
BstMAI	GTCTC	3 cut(s)	54, 221, 2648
BstMCI	CGRYCG	1 cut(s)	4774
BstNI	CCWGG	9 cut(s)	70, 794, 1149, 3798, 4383, 4500, 4537, 4608, 4708
BstNSI	RCATGY	6 cut(s)	913, 1206, 1691, 2759, 2889, 5033
BstPI	GGTNACC	2 cut(s)	796, 4507
BstSFI	CTRYAG	9 cut(s)	1302, 1383, 1391, 2496, 2953, 3486, 3534, 3656, 4139
BstSLI	GKGCMC	2 cut(s)	3415, 3874
BstUI	CGCG	1 cut(s)	241
BstV2I	GAAGAC	5 cut(s)	732, 963, 1754, 2768, 4860
BstX2I	RGATCY	3 cut(s)	848, 1262, 5013
BstXI	CCANNNNNNTGG	1 cut(s)	3371
BstYI	RGATCY	3 cut(s)	848, 1262, 5013
BstZ17I	GTATAC	1 cut(s)	2382
Bsu15I	ATCGAT	1 cut(s)	180
BsuI	GTATCC	1 cut(s)	3560
BsuRI	GGCC	7 cut(s)	73, 171, 176, 1555, 1837, 3872, 4343
BsuTUI	ATCGAT	1 cut(s)	180
BtgI	CCRYGG	2 cut(s)	1005, 4021
BtsI	GCAGTG	2 cut(s)	1299, 4864
BveI	ACCTGC	2 cut(s)	641, 911
Cac8I	GCNNGC	8 cut(s)	1495, 1557, 2015, 2857, 2887, 4043, 4666, 4778
CaiI	CAGNNNCTG	1 cut(s)	1955
CciI	TCATGA	1 cut(s)	4335
CfoI	GCGC	2 cut(s)	704, 4985
Cfr13I	GGNCC	6 cut(s)	170, 1553, 3243, 3606, 3870, 3871
ClaI	ATCGAT	1 cut(s)	180
CsiI	ACCWGGT	2 cut(s)	792, 4706
CspCI	CAANNNNNGTGG	6 cut(s)	1344, 1379, 2608, 2643, 3697, 3732
DraI	TTTAAA	2 cut(s)	2874, 3385
DraIII	CACNNNGTG	4 cut(s)	1550, 2741, 2999, 4097
DriI	GACNNNNNGTC	2 cut(s)	625, 1855
EaeI	YGGCCR	2 cut(s)	71, 174
Eam1104I	CTCTTC	2 cut(s)	1546, 3144
Eam1105I	GACNNNNNGTC	2 cut(s)	625, 1855
EarI	CTCTTC	2 cut(s)	1546, 3144
EciI	GGCGGA	2 cut(s)	113, 1731
Ecl136II	GAGCTC	1 cut(s)	3188
Eco130I	CCWWGG	7 cut(s)	141, 441, 1005, 3250, 3636, 3922, 4021
Eco147I	AGGCCT	1 cut(s)	4343
Eco24I	GRGCYC	3 cut(s)	3190, 3224, 3874
Eco31I	GGTCTC	1 cut(s)	54
Eco32I	GATATC	1 cut(s)	184
Eco47I	GGWCC	2 cut(s)	3243, 3606
Eco53kI	GAGCTC	1 cut(s)	3188
Eco57I	CTGAAG	5 cut(s)	263, 1917, 4422, 4580, 4668
Eco88I	CYCGRG	1 cut(s)	489
Eco91I	GGTNACC	2 cut(s)	796, 4507
EcoICRI	GAGCTC	1 cut(s)	3188
EcoO65I	GGTNACC	2 cut(s)	796, 4507
EcoRI	GAATTC	3 cut(s)	339, 561, 3970
EcoRII	CCWGG	9 cut(s)	68, 792, 1147, 3796, 4381, 4498, 4535, 4606, 4706
EcoRV	GATATC	1 cut(s)	184
EcoT14I	CCWWGG	7 cut(s)	141, 441, 1005, 3250, 3636, 3922, 4021
EcoT22I	ATGCAT	2 cut(s)	2751, 2788
EcoT38I	GRGCYC	3 cut(s)	3190, 3224, 3874
ErhI	CCWWGG	7 cut(s)	141, 441, 1005, 3250, 3636, 3922, 4021
FalI	AAGNNNNNCTT	4 cut(s)	753, 785, 3120, 3152
FaqI	GGGAC	2 cut(s)	3445, 3504
FauI	CCCGC	1 cut(s)	122
FauNDI	CATATG	2 cut(s)	3174, 4681
FbaI	TGATCA	1 cut(s)	1924
FblI	GTMKAC	1 cut(s)	2381
FriOI	GRGCYC	3 cut(s)	3190, 3224, 3874
GlaI	GCGC	2 cut(s)	703, 4984
GsaI	CCCAGC	2 cut(s)	2442, 3877
GsuI	CTGGAG	6 cut(s)	896, 1131, 1422, 4206, 4737, 4992
HaeII	RGCGCY	1 cut(s)	705
HaeIII	GGCC	7 cut(s)	73, 171, 176, 1555, 1837, 3872, 4343
HapII	CCGG	4 cut(s)	173, 254, 404, 2590
HhaI	GCGC	2 cut(s)	704, 4985
Hin6I	GCGC	2 cut(s)	702, 4983
HinP1I	GCGC	2 cut(s)	702, 4983
HincII	GTYRAC	2 cut(s)	1903, 2068
HindII	GTYRAC	2 cut(s)	1903, 2068
HindIII	AAGCTT	3 cut(s)	1877, 3088, 4562
HpaII	CCGG	4 cut(s)	173, 254, 404, 2590
HphI	GGTGA	6 cut(s)	826, 990, 1536, 3102, 4328, 4427
Hpy166II	GTNNAC	9 cut(s)	901, 1429, 1903, 2068, 2119, 2382, 2575, 4182, 5027
Hpy8I	GTNNAC	9 cut(s)	901, 1429, 1903, 2068, 2119, 2382, 2575, 4182, 5027
Hpy99I	CGWCG	3 cut(s)	170, 209, 212
HpyCH4IV	ACGT	5 cut(s)	1130, 2344, 2386, 2585, 4093
HpySE526I	ACGT	5 cut(s)	1130, 2344, 2386, 2585, 4093
HspAI	GCGC	2 cut(s)	702, 4983
Kpn2I	TCCGGA	1 cut(s)	2589
KpnI	GGTACC	1 cut(s)	3036
Ksp22I	TGATCA	1 cut(s)	1924
LguI	GCTCTTC	1 cut(s)	3144
MabI	ACCWGGT	2 cut(s)	792, 4706
MaeII	ACGT	5 cut(s)	1130, 2344, 2386, 2585, 4093
MfeI	CAATTG	1 cut(s)	2568
MflI	RGATCY	3 cut(s)	848, 1262, 5013
MhlI	GDGCHC	7 cut(s)	2192, 3004, 3190, 3224, 3415, 3874, 4666
MmeI	TCCRAC	9 cut(s)	63, 710, 1008, 2751, 3270, 3352, 3544, 4498, 4873
Mph1103I	ATGCAT	2 cut(s)	2751, 2788
MroI	TCCGGA	1 cut(s)	2589
MroXI	GAANNNNTTC	5 cut(s)	765, 889, 2043, 3135, 3675
MslI	CAYNNNNRTG	8 cut(s)	693, 908, 1979, 2628, 2754, 2884, 3173, 4788
MspA1I	CMGCKG	5 cut(s)	1373, 2306, 2483, 4041, 4840
MspCI	CTTAAG	1 cut(s)	4735
MspI	CCGG	4 cut(s)	173, 254, 404, 2590
MunI	CAATTG	1 cut(s)	2568
Mva1269I	GAATGC	1 cut(s)	2994
MvaI	CCWGG	9 cut(s)	70, 794, 1149, 3798, 4383, 4500, 4537, 4608, 4708
MvnI	CGCG	1 cut(s)	241
NciI	CCSGG	1 cut(s)	173
NcoI	CCATGG	2 cut(s)	1005, 4021
NdeI	CATATG	2 cut(s)	3174, 4681
NlaIV	GGNNCC	9 cut(s)	966, 1584, 3034, 3337, 3634, 3672, 3872, 4396, 4534
NmeAIII	GCCGAG	1 cut(s)	393
NmuCI	GTSAC	3 cut(s)	193, 2084, 5083
NsiI	ATGCAT	2 cut(s)	2751, 2788
NspI	RCATGY	6 cut(s)	913, 1206, 1691, 2759, 2889, 5033
PaeI	GCATGC	1 cut(s)	2889
PaeR7I	CTCGAG	1 cut(s)	489
PagI	TCATGA	1 cut(s)	4335
PaqCI	CACCTGC	1 cut(s)	911
PceI	AGGCCT	1 cut(s)	4343
PciI	ACATGT	1 cut(s)	1202
PciSI	GCTCTTC	1 cut(s)	3144
PcsI	WCGNNNNNNNCGW	2 cut(s)	153, 4090
PctI	GAATGC	1 cut(s)	2994
PdmI	GAANNNNTTC	5 cut(s)	765, 889, 2043, 3135, 3675
PflMI	CCANNNNNTGG	5 cut(s)	69, 2968, 3889, 4097, 5024
PfoI	TCCNGGA	2 cut(s)	1147, 3796
Ppu21I	YACGTR	3 cut(s)	2345, 2387, 4094
PscI	ACATGT	1 cut(s)	1202
PsiI	TTATAA	2 cut(s)	2409, 3773
Psp124BI	GAGCTC	1 cut(s)	3190
Psp6I	CCWGG	9 cut(s)	68, 792, 1147, 3796, 4381, 4498, 4535, 4606, 4706
PspEI	GGTNACC	2 cut(s)	796, 4507
PspFI	CCCAGC	2 cut(s)	2438, 3873
PspGI	CCWGG	9 cut(s)	68, 792, 1147, 3796, 4381, 4498, 4535, 4606, 4706
PspN4I	GGNNCC	9 cut(s)	966, 1584, 3034, 3337, 3634, 3672, 3872, 4396, 4534
PspOMI	GGGCCC	1 cut(s)	3870
PspPI	GGNCC	6 cut(s)	170, 1553, 3243, 3606, 3870, 3871
PspXI	VCTCGAGB	1 cut(s)	489
PstI	CTGCAG	8 cut(s)	1306, 1387, 1395, 2957, 3490, 3538, 3660, 4143
PstNI	CAGNNNCTG	1 cut(s)	1955
PsuI	RGATCY	3 cut(s)	848, 1262, 5013
PvuII	CAGCTG	4 cut(s)	1373, 2306, 2483, 4840
RseI	CAYNNNNRTG	8 cut(s)	693, 908, 1979, 2628, 2754, 2884, 3173, 4788
SacI	GAGCTC	1 cut(s)	3190
SapI	GCTCTTC	1 cut(s)	3144
Sau96I	GGNCC	6 cut(s)	170, 1553, 3243, 3606, 3870, 3871
ScaI	AGTACT	3 cut(s)	2517, 2869, 2975
SduI	GDGCHC	7 cut(s)	2192, 3004, 3190, 3224, 3415, 3874, 4666
SexAI	ACCWGGT	2 cut(s)	792, 4706
SfcI	CTRYAG	9 cut(s)	1302, 1383, 1391, 2496, 2953, 3486, 3534, 3656, 4139
Sfr274I	CTCGAG	1 cut(s)	489
SinI	GGWCC	2 cut(s)	3243, 3606
SlaI	CTCGAG	1 cut(s)	489
SmiI	ATTTAAAT	1 cut(s)	3385
SmiMI	CAYNNNNRTG	8 cut(s)	693, 908, 1979, 2628, 2754, 2884, 3173, 4788
SmlI	CTYRAG	4 cut(s)	489, 2271, 4073, 4735
SmoI	CTYRAG	4 cut(s)	489, 2271, 4073, 4735
SpeI	ACTAGT	1 cut(s)	1886
SphI	GCATGC	1 cut(s)	2889
SseBI	AGGCCT	1 cut(s)	4343
SspI	AATATT	3 cut(s)	2637, 2690, 3754
SstI	GAGCTC	1 cut(s)	3190
StuI	AGGCCT	1 cut(s)	4343
StyI	CCWWGG	7 cut(s)	141, 441, 1005, 3250, 3636, 3922, 4021
SwaI	ATTTAAAT	1 cut(s)	3385
TaiI	ACGT	5 cut(s)	1133, 2347, 2389, 2588, 4096
TatI	WGTACW	9 cut(s)	749, 1525, 1681, 2477, 2515, 2867, 2973, 3983, 4429
TauI	GCSGC	6 cut(s)	76, 79, 82, 85, 88, 367
TseFI	GTSAC	3 cut(s)	193, 2084, 5083
Tsp45I	GTSAC	3 cut(s)	193, 2084, 5083
TspGWI	ACGGA	3 cut(s)	212, 957, 2708
Van91I	CCANNNNNTGG	5 cut(s)	69, 2968, 3889, 4097, 5024
Vha464I	CTTAAG	1 cut(s)	4735
VpaK11BI	GGWCC	2 cut(s)	3243, 3606
XbaI	TCTAGA	3 cut(s)	3139, 4658, 4699
XceI	RCATGY	6 cut(s)	913, 1206, 1691, 2759, 2889, 5033
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2555
XhoI	CTCGAG	1 cut(s)	489
XmaJI	CCTAGG	1 cut(s)	3922
XmiI	GTMKAC	1 cut(s)	2381
XmnI	GAANNNNTTC	5 cut(s)	765, 889, 2043, 3135, 3675
ZrmI	AGTACT	3 cut(s)	2517, 2869, 2975
Zsp2I	ATGCAT	2 cut(s)	2751, 2788

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.