MD13G1010600.v1.1

Tetratricopeptide repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007638
Physical Location & Seq
Forward (+)
642288 .. 643301
1014 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1010600.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGAACTCCTCCTCCACTCTCGCCTCCTCCTCTTCATCCTCCTCTTTCTTCCAATACAAACGCCTTCCCACTAAACCAACCCAACCTTCTCATCTCCGTTTTGCCCCTCCCAATTCCCACAATGTCCCTGCCCTCAAAGTCACAGCCTCCTCCTCCGCCCACCACCACCGCACCCTCCTCACAGGTAACTCCCACAGCAACCCAAACCCGATTCTCCAAACTCTCAAACGCTACGCCAAAGCGGCCGTTCTCATCGGAGTCACCGCCGCCGTGTTCACCACCAAGGCCTCGACTTTGCTCGCCAGAGCCGAACCTCCGCCTGCATTGACCGAAGAAGCGCCGACCCCGGTCACAGATGACGAGAACAACCAATCTTCATCGCCATTATCCGACTTCCTGGGTTCGAATTCGGAAGCCATCGATGCCCTGAAATCGCTTCTGCAGCAGAAGCTCGAAAATGGCGAGGACGACGAGGCGCTCAAGATCCTGCAGCGCTTGGTCGCGGCGCAACCCTCCGCCACCGAGTGGAAATTCATGATGGCTAGAGTTCTCAGCGAAATGGGCGAAAACGAAAGTGCCCGCCGAGTGTTCGAGGAAATACTCGCCTCGAACCCCTTGTCATTCGAGGCGTTGTTCGAGAACGCCCTGTTGATGGACCGCTGCGGCGAAGGCGAGGCCGTGATAAAGCGATTGGAGGACGCATTGCAGGTTGCGGAGGAGGAGAACAAGGCGAAGGAGGCTCGAGACGTTAAATTGATAATGGCGCAGGTTCAGTTCCTGCAGAAGAATGTGGAGGACGCATTGAATAGCTACAATGAATTGGTAAAGGAGGACCCGAAAGACTTGCGGCCGTACTTTTGCCGTGGGATGATTTACAGTTTGCTTGATCGGAATGCGGAGGCGACGGAGCAGTTTGAGAAGTACCGGCAGCTTTCCCCGAAGAAGTTCGAGGTGGATGGCTACTTGAGGAAGCCATTGTCGAGAGTCAAGGTATTCGGGAGTGACGAGAATTGA

Protein Analysis

338

Amino Acids

37.44

Weight (kDa)

5.68

Isoelectric Point (pI)

47.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_19 PF14559 151 - 206 1.2e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 697, 757
AccB7I CCANNNNNTGG 1 cut(s) 525
AccII CGCG 1 cut(s) 503
AclWI GGATC 1 cut(s) 478
AcoI YGGCCR 2 cut(s) 243, 848
AcsI RAATTY 2 cut(s) 406, 530
AdeI CACNNNGTG 1 cut(s) 525
AfaI GTAC 2 cut(s) 854, 923
AfeI AGCGCT 1 cut(s) 494
AfiI CCNNNNNNNGG 2 cut(s) 525, 652
AgsI TTSAA 1 cut(s) 805
AjnI CCWGG 1 cut(s) 396
AjuI GAANNNNNNNTTGG 2 cut(s) 70, 102
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 3 cut(s) 451, 810, 931
AluI AGCT 3 cut(s) 451, 810, 931
Alw26I GTCTC 1 cut(s) 738
AlwI GGATC 1 cut(s) 478
AlwNI CAGNNNCTG 1 cut(s) 778
Ama87I CYCGRG 1 cut(s) 741
Aor51HI AGCGCT 1 cut(s) 494
AoxI GGCC 4 cut(s) 243, 285, 675, 848
ApeKI GCWGC 4 cut(s) 442, 490, 660, 928
ApoI RAATTY 2 cut(s) 406, 530
ArsI GACNNNNNNTTYG 2 cut(s) 602, 634
Asp700I GAANNNNTTC 1 cut(s) 944
AspLEI GCGC 5 cut(s) 340, 478, 495, 508, 766
AspS9I GGNCC 2 cut(s) 655, 832
AsuC2I CCSGG 1 cut(s) 347
AsuHPI GGTGA 2 cut(s) 253, 268
AsuII TTCGAA 1 cut(s) 404
AvaI CYCGRG 1 cut(s) 741
AvaII GGWCC 2 cut(s) 655, 832
BaeGI GKGCMC 1 cut(s) 580
BbvI GCAGC 4 cut(s) 454, 502, 647, 940
BccI CCATC 4 cut(s) 425, 532, 646, 950
BceAI ACGGC 5 cut(s) 230, 254, 662, 835, 846
BcgI CGANNNNNNTGC 2 cut(s) 826, 860
BciT130I CCWGG 1 cut(s) 398
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 1 cut(s) 738
BfaI CTAG 1 cut(s) 543
BfmI CTRYAG 3 cut(s) 440, 488, 779
BfoI RGCGCY 3 cut(s) 341, 479, 496
BfuAI ACCTGC 2 cut(s) 697, 757
BglI GCCNNNNNGGC 1 cut(s) 242
BisI GCNGC 9 cut(s) 243, 267, 443, 491, 504, 661, 664, 848, 929
BlsI GCNGC 9 cut(s) 244, 268, 444, 492, 505, 662, 665, 849, 930
Bme1390I CCNGG 2 cut(s) 347, 398
Bme18I GGWCC 2 cut(s) 655, 832
BmeT110I CYCGRG 1 cut(s) 741
BmgT120I GGNCC 2 cut(s) 655, 832
BmiI GGNNCC 1 cut(s) 834
BmrFI CCNGG 2 cut(s) 347, 398
BmsI GCATC 1 cut(s) 412
BoxI GACNNNNGTC 1 cut(s) 347
Bpu14I TTCGAA 1 cut(s) 404
BpuEI CTTGAG 2 cut(s) 464, 985
BpuMI CCSGG 1 cut(s) 347
Bsa29I ATCGAT 1 cut(s) 420
BsaJI CCNNGG 4 cut(s) 282, 345, 397, 862
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 525, 652
Bse118I RCCGGY 1 cut(s) 924
Bse3DI GCAATG 1 cut(s) 701
BseBI CCWGG 1 cut(s) 398
BseCI ATCGAT 1 cut(s) 420
BseDI CCNNGG 4 cut(s) 282, 345, 397, 862
BseGI GGATG 3 cut(s) 35, 873, 961
BseLI CCNNNNNNNGG 2 cut(s) 525, 652
BseMI GCAATG 1 cut(s) 701
BseMII CTCAG 1 cut(s) 565
BseRI GAGGAG 8 cut(s) 16, 19, 31, 139, 142, 167, 731, 734
BseSI GKGCMC 1 cut(s) 580
BseX3I CGGCCG 2 cut(s) 243, 848
BseXI GCAGC 4 cut(s) 454, 502, 647, 940
Bsh1236I CGCG 1 cut(s) 503
Bsh1285I CGRYCG 2 cut(s) 246, 851
BshFI GGCC 4 cut(s) 245, 287, 677, 850
BshVI ATCGAT 1 cut(s) 420
BsiEI CGRYCG 2 cut(s) 246, 851
BsiHKCI CYCGRG 1 cut(s) 741
BsiSI CCGG 2 cut(s) 347, 925
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 2 cut(s) 525, 652
BsmAI GTCTC 1 cut(s) 738
BsmBI CGTCTC 1 cut(s) 738
BsmFI GGGAC 1 cut(s) 110
BsmI GAATGC 1 cut(s) 898
BsnI GGCC 4 cut(s) 245, 287, 677, 850
BsoBI CYCGRG 1 cut(s) 741
Bsp119I TTCGAA 1 cut(s) 404
Bsp1286I GDGCHC 1 cut(s) 580
Bsp143I GATC 2 cut(s) 483, 886
BspANI GGCC 4 cut(s) 245, 287, 677, 850
BspCNI CTCAG 1 cut(s) 564
BspDI ATCGAT 1 cut(s) 420
BspFNI CGCG 1 cut(s) 503
BspHI TCATGA 1 cut(s) 534
BspLI GGNNCC 1 cut(s) 834
BspMAI CTGCAG 3 cut(s) 444, 492, 783
BspMI ACCTGC 2 cut(s) 697, 757
BspPI GGATC 1 cut(s) 478
BspT104I TTCGAA 1 cut(s) 404
BsrDI GCAATG 1 cut(s) 701
BsrFI RCCGGY 1 cut(s) 924
BssAI RCCGGY 1 cut(s) 924
BssECI CCNNGG 4 cut(s) 282, 345, 397, 862
BssMI GATC 2 cut(s) 483, 886
BssT1I CCWWGG 1 cut(s) 282
Bst2UI CCWGG 1 cut(s) 398
Bst4CI ACNGT 1 cut(s) 878
Bst6I CTCTTC 1 cut(s) 37
BstBI TTCGAA 1 cut(s) 404
BstC8I GCNNGC 3 cut(s) 300, 321, 580
BstDEI CTNAG 1 cut(s) 551
BstDSI CCRYGG 1 cut(s) 862
BstF5I GGATG 3 cut(s) 35, 873, 961
BstFNI CGCG 1 cut(s) 503
BstH2I RGCGCY 3 cut(s) 341, 479, 496
BstHHI GCGC 5 cut(s) 340, 478, 495, 508, 766
BstKTI GATC 2 cut(s) 486, 889
BstMAI GTCTC 1 cut(s) 738
BstMBI GATC 2 cut(s) 483, 886
BstMCI CGRYCG 2 cut(s) 246, 851
BstMWI GCNNNNNNNGC 7 cut(s) 242, 422, 442, 448, 561, 669, 737
BstNI CCWGG 1 cut(s) 398
BstPAI GACNNNNGTC 1 cut(s) 347
BstSCI CCNGG 2 cut(s) 345, 396
BstSFI CTRYAG 3 cut(s) 440, 488, 779
BstSLI GKGCMC 1 cut(s) 580
BstUI CGCG 1 cut(s) 503
BstV1I GCAGC 4 cut(s) 454, 502, 647, 940
BstX2I RGATCY 1 cut(s) 483
BstYI RGATCY 1 cut(s) 483
BstZI CGGCCG 2 cut(s) 243, 848
Bsu15I ATCGAT 1 cut(s) 420
BsuRI GGCC 4 cut(s) 245, 287, 677, 850
BsuTUI ATCGAT 1 cut(s) 420
BtgI CCRYGG 1 cut(s) 862
BtgZI GCGATG 1 cut(s) 363
BtsCI GGATG 3 cut(s) 35, 873, 961
BveI ACCTGC 2 cut(s) 697, 757
Cac8I GCNNGC 3 cut(s) 300, 321, 580
CaiI CAGNNNCTG 1 cut(s) 778
CciI TCATGA 1 cut(s) 534
CfoI GCGC 5 cut(s) 340, 478, 495, 508, 766
Cfr10I RCCGGY 1 cut(s) 924
Cfr13I GGNCC 2 cut(s) 655, 832
ClaI ATCGAT 1 cut(s) 420
CseI GACGC 2 cut(s) 707, 806
Csp6I GTAC 2 cut(s) 853, 922
CviAII CATG 1 cut(s) 535
CviQI GTAC 2 cut(s) 853, 922
DdeI CTNAG 1 cut(s) 551
DpnI GATC 2 cut(s) 485, 888
DpnII GATC 2 cut(s) 483, 886
DraIII CACNNNGTG 1 cut(s) 525
EaeI YGGCCR 2 cut(s) 243, 848
EagI CGGCCG 2 cut(s) 243, 848
Eam1104I CTCTTC 1 cut(s) 37
EarI CTCTTC 1 cut(s) 37
EciI GGCGGA 3 cut(s) 145, 306, 505
EclXI CGGCCG 2 cut(s) 243, 848
Eco130I CCWWGG 1 cut(s) 282
Eco147I AGGCCT 1 cut(s) 287
Eco47I GGWCC 2 cut(s) 655, 832
Eco47III AGCGCT 1 cut(s) 494
Eco52I CGGCCG 2 cut(s) 243, 848
Eco88I CYCGRG 1 cut(s) 741
EcoO109I RGGNCCY 1 cut(s) 832
EcoRI GAATTC 1 cut(s) 406
EcoRII CCWGG 1 cut(s) 396
EcoT14I CCWWGG 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 282
Esp3I CGTCTC 1 cut(s) 738
FaeI CATG 1 cut(s) 538
FaiI YATR 1 cut(s) 536
FaqI GGGAC 1 cut(s) 110
FatI CATG 1 cut(s) 534
FauI CCCGC 1 cut(s) 587
Fnu4HI GCNGC 9 cut(s) 243, 267, 443, 491, 504, 661, 664, 848, 929
FokI GGATG 3 cut(s) 22, 880, 968
Fsp4HI GCNGC 9 cut(s) 243, 267, 443, 491, 504, 661, 664, 848, 929
FspBI CTAG 1 cut(s) 543
GlaI GCGC 5 cut(s) 339, 477, 494, 507, 765
GluI GCNGC 9 cut(s) 243, 267, 443, 491, 504, 661, 664, 848, 929
HaeII RGCGCY 3 cut(s) 341, 479, 496
HaeIII GGCC 4 cut(s) 245, 287, 677, 850
HapII CCGG 2 cut(s) 347, 925
HgaI GACGC 2 cut(s) 707, 806
HhaI GCGC 5 cut(s) 340, 478, 495, 508, 766
Hin1II CATG 1 cut(s) 538
Hin6I GCGC 5 cut(s) 338, 476, 493, 506, 764
HinP1I GCGC 5 cut(s) 338, 476, 493, 506, 764
HinfI GANTC 3 cut(s) 211, 258, 984
HpaII CCGG 2 cut(s) 347, 925
HphI GGTGA 2 cut(s) 253, 268
Hpy166II GTNNAC 1 cut(s) 276
Hpy188I TCNGA 4 cut(s) 257, 391, 412, 891
Hpy188III TCNNGA 6 cut(s) 481, 535, 637, 743, 981, 997
Hpy8I GTNNAC 1 cut(s) 276
Hpy99I CGWCG 2 cut(s) 473, 907
HpyAV CCTTC 4 cut(s) 74, 96, 662, 727
HpyCH4III ACNGT 1 cut(s) 878
HpyCH4IV ACGT 1 cut(s) 747
HpyCH4V TGCA 5 cut(s) 323, 442, 490, 706, 781
HpyF10VI GCNNNNNNNGC 7 cut(s) 242, 422, 442, 448, 561, 669, 737
HpyF3I CTNAG 1 cut(s) 551
HpySE526I ACGT 1 cut(s) 747
Hsp92II CATG 1 cut(s) 538
HspAI GCGC 5 cut(s) 338, 476, 493, 506, 764
Kzo9I GATC 2 cut(s) 483, 886
LmnI GCTCC 1 cut(s) 907
Lsp1109I GCAGC 4 cut(s) 454, 502, 647, 940
LweI GCATC 1 cut(s) 412
MaeI CTAG 1 cut(s) 543
MaeII ACGT 1 cut(s) 747
MaeIII GTNAC 5 cut(s) 139, 185, 259, 349, 1001
MalI GATC 2 cut(s) 485, 888
MboI GATC 2 cut(s) 483, 886
MboII GAAGA 6 cut(s) 24, 40, 344, 366, 796, 952
MflI RGATCY 1 cut(s) 483
MhlI GDGCHC 1 cut(s) 580
MluCI AATT 6 cut(s) 112, 406, 530, 752, 818, 1009
MlyI GAGTC 2 cut(s) 267, 993
MmeI TCCRAC 1 cut(s) 414
MroXI GAANNNNTTC 1 cut(s) 944
MseI TTAA 1 cut(s) 750
MspA1I CMGCKG 1 cut(s) 660
MspI CCGG 2 cut(s) 347, 925
MspR9I CCNGG 2 cut(s) 347, 398
Mva1269I GAATGC 1 cut(s) 898
MvaI CCWGG 1 cut(s) 398
MvnI CGCG 1 cut(s) 503
MwoI GCNNNNNNNGC 7 cut(s) 242, 422, 442, 448, 561, 669, 737
NciI CCSGG 1 cut(s) 347
NdeII GATC 2 cut(s) 483, 886
NlaIII CATG 1 cut(s) 538
NlaIV GGNNCC 1 cut(s) 834
NmeAIII GCCGAG 1 cut(s) 608
NmuCI GTSAC 4 cut(s) 139, 259, 349, 1001
NspV TTCGAA 1 cut(s) 404
PaeR7I CTCGAG 1 cut(s) 741
PagI TCATGA 1 cut(s) 534
PceI AGGCCT 1 cut(s) 287
PcsI WCGNNNNNNNCGW 3 cut(s) 306, 459, 1002
PctI GAATGC 1 cut(s) 898
PdmI GAANNNNTTC 1 cut(s) 944
PfeI GAWTC 1 cut(s) 211
PflMI CCANNNNNTGG 1 cut(s) 525
PkrI GCNGC 9 cut(s) 244, 268, 444, 492, 505, 662, 665, 849, 930
PleI GAGTC 2 cut(s) 266, 992
PpsI GAGTC 2 cut(s) 266, 992
PpuMI RGGWCCY 1 cut(s) 832
PshAI GACNNNNGTC 1 cut(s) 347
Psp5II RGGWCCY 1 cut(s) 832
Psp6I CCWGG 1 cut(s) 396
PspGI CCWGG 1 cut(s) 396
PspN4I GGNNCC 1 cut(s) 834
PspPI GGNCC 2 cut(s) 655, 832
PspPPI RGGWCCY 1 cut(s) 832
PstI CTGCAG 3 cut(s) 444, 492, 783
PstNI CAGNNNCTG 1 cut(s) 778
PsuI RGATCY 1 cut(s) 483
RsaI GTAC 2 cut(s) 854, 923
RsaNI GTAC 2 cut(s) 853, 922
SaqAI TTAA 1 cut(s) 750
SatI GCNGC 9 cut(s) 243, 267, 443, 491, 504, 661, 664, 848, 929
Sau3AI GATC 2 cut(s) 483, 886
Sau96I GGNCC 2 cut(s) 655, 832
SchI GAGTC 2 cut(s) 267, 993
ScrFI CCNGG 2 cut(s) 347, 398
SduI GDGCHC 1 cut(s) 580
SfaNI GCATC 1 cut(s) 412
SfcI CTRYAG 3 cut(s) 440, 488, 779
Sfr274I CTCGAG 1 cut(s) 741
SfuI TTCGAA 1 cut(s) 404
SinI GGWCC 2 cut(s) 655, 832
SlaI CTCGAG 1 cut(s) 741
SmlI CTYRAG 3 cut(s) 479, 741, 964
SmoI CTYRAG 3 cut(s) 479, 741, 964
Sse9I AATT 6 cut(s) 112, 406, 530, 752, 818, 1009
SseBI AGGCCT 1 cut(s) 287
SspMI CTAG 1 cut(s) 543
StuI AGGCCT 1 cut(s) 287
StyD4I CCNGG 2 cut(s) 345, 396
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 1 cut(s) 878
TaiI ACGT 1 cut(s) 750
TaqII GACCGA 1 cut(s) 344
TasI AATT 6 cut(s) 112, 406, 530, 752, 818, 1009
TauI GCSGC 5 cut(s) 245, 269, 506, 666, 850
TfiI GAWTC 1 cut(s) 211
Tru1I TTAA 1 cut(s) 750
Tru9I TTAA 1 cut(s) 750
TseFI GTSAC 4 cut(s) 139, 259, 349, 1001
TseI GCWGC 4 cut(s) 442, 490, 660, 928
Tsp45I GTSAC 4 cut(s) 139, 259, 349, 1001
TspDTI ATGAA 5 cut(s) 17, 24, 366, 523, 831
TspGWI ACGGA 2 cut(s) 86, 920
Van91I CCANNNNNTGG 1 cut(s) 525
VpaK11BI GGWCC 2 cut(s) 655, 832
XapI RAATTY 2 cut(s) 406, 530
XhoI CTCGAG 1 cut(s) 741
XmnI GAANNNNTTC 1 cut(s) 944
XspI CTAG 1 cut(s) 543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.