pycom13g00870

Tetratricopeptide repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007638
Physical Location & Seq
Forward (+)
541320 .. 542339
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g00870.1

Sequence Viewer

Length: 1020 bp
ATGAACTCCTCCTCCACTCTCGCCTCCTCTTCATCCTCCTCTTTCTTCCAATACAAACACCTTCCCACTAAACCAACCCAACCTTATCTTCTCCGTTTTGCCCCTCCCAATTCCCACAATTCACACAATGTCCCTGCCCTCAAAGTCACAGCCTCCTCCTCCGCCCCCCACCACCGCAGCCTCCTCACAGGAAACTCCCACAGCAACCCGAACCCGATTCTCCAAACTCTCAAACGCTACGCCAAAGCGGCCATTCTCATCGGAGTCACCGCCGCCGTGTTCACCACCAAGTCCTCGACTTTGCTCGCTAGAGCCGAACCTCCGCCTGCATTGACCGAAGAAGCGCCGACCCAGGTCACAGATGACGAGAACAACCAATCCTCATCGCCATTGTCCGACTTCCTGGGTTCGAATTCGGAAGCCATCGATGCCCTGAAATCGCTTCTGCAGCAGAAGCTCGAAAACGGCGAGGATGACGAGGCGCTCAAGATCCTGCAGCGCTTGGTCGCGGCGCAACCCTCTGCCACCGAGTGGAAATTCATGATGGCTAGGGTTCTCAGCGAAATGGGCGAAAACGAAAGTGCCCGCCAAGTGTTCGAGGAAATACTCGCCTCGAACCCCTTGTCATTCGAGGCGTTGTTCGAGAACGCCCTGCTGATGGACCGCTGCGGCGAAGGCGAGGCCGTGATAAAGCGATTGGAGGATTCATTGCAGGTTGCGGAGGAGGAGAACAAGGCGAAGGAGGCTCGAGACGTTAAATTGATAATGGCGCAGGTTCAGTTCCTGCAGAAGAATGTGGATGACGCATTGAATAGCTACAATGAATTGGTAAAGGAGGACCCGAAAGACTTCCGGCCGTACTTTTGCCGTGGGATGATTTACAGTTTGCTTGATCGGAATGCGGAGGCAACGGAGCAGTTTGAGAAGTACCGGCAGCTTTTGCCGAAGAAGTTCGAGGTGGATGGCTACTTGAGGAAGCCATTGTCGAGAGTCAAGGTATTCGGGAGTGACGAGAATTGA

Protein Analysis

340

Amino Acids

37.77

Weight (kDa)

5.45

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_19 PF14559 153 - 208 5.8e-07 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 703, 763
AccB7I CCANNNNNTGG 1 cut(s) 531
AccII CGCG 1 cut(s) 509
AclWI GGATC 1 cut(s) 484
AcoI YGGCCR 2 cut(s) 249, 854
AcsI RAATTY 2 cut(s) 412, 536
AdeI CACNNNGTG 1 cut(s) 531
AfaI GTAC 2 cut(s) 860, 929
AfeI AGCGCT 1 cut(s) 500
AfiI CCNNNNNNNGG 2 cut(s) 531, 658
AgsI TTSAA 1 cut(s) 811
AjnI CCWGG 2 cut(s) 351, 402
AjuI GAANNNNNNNTTGG 2 cut(s) 72, 104
AloI GAACNNNNNNTCC 3 cut(s) 28, 362, 394
AluBI AGCT 3 cut(s) 457, 816, 937
AluI AGCT 3 cut(s) 457, 816, 937
Alw26I GTCTC 1 cut(s) 744
AlwI GGATC 1 cut(s) 484
AlwNI CAGNNNCTG 1 cut(s) 784
Ama87I CYCGRG 1 cut(s) 747
Aor51HI AGCGCT 1 cut(s) 500
AoxI GGCC 3 cut(s) 249, 681, 854
ApeKI GCWGC 5 cut(s) 177, 448, 496, 666, 934
ApoI RAATTY 2 cut(s) 412, 536
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
Asp700I GAANNNNTTC 2 cut(s) 848, 950
AspLEI GCGC 5 cut(s) 346, 484, 501, 514, 772
AspS9I GGNCC 2 cut(s) 661, 838
AsuHPI GGTGA 2 cut(s) 259, 274
AsuII TTCGAA 1 cut(s) 410
AvaI CYCGRG 1 cut(s) 747
AvaII GGWCC 2 cut(s) 661, 838
BaeGI GKGCMC 1 cut(s) 586
BbvI GCAGC 5 cut(s) 189, 460, 508, 653, 946
BccI CCATC 4 cut(s) 431, 538, 652, 956
BceAI ACGGC 5 cut(s) 260, 481, 668, 841, 852
BciT130I CCWGG 2 cut(s) 353, 404
BcoDI GTCTC 1 cut(s) 744
BfaI CTAG 2 cut(s) 309, 549
BfmI CTRYAG 3 cut(s) 446, 494, 785
BfoI RGCGCY 3 cut(s) 347, 485, 502
BfuAI ACCTGC 2 cut(s) 703, 763
BglI GCCNNNNNGGC 1 cut(s) 248
BisI GCNGC 9 cut(s) 178, 249, 273, 449, 497, 510, 667, 670, 935
BlsI GCNGC 9 cut(s) 179, 250, 274, 450, 498, 511, 668, 671, 936
Bme1390I CCNGG 2 cut(s) 353, 404
Bme18I GGWCC 2 cut(s) 661, 838
BmeT110I CYCGRG 1 cut(s) 747
BmgT120I GGNCC 2 cut(s) 661, 838
BmiI GGNNCC 1 cut(s) 840
BmrFI CCNGG 2 cut(s) 353, 404
BmsI GCATC 1 cut(s) 418
BoxI GACNNNNGTC 1 cut(s) 353
Bpu14I TTCGAA 1 cut(s) 410
BpuEI CTTGAG 2 cut(s) 470, 991
Bsa29I ATCGAT 1 cut(s) 426
BsaJI CCNNGG 3 cut(s) 351, 403, 868
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 531, 658
Bse118I RCCGGY 1 cut(s) 930
Bse3DI GCAATG 1 cut(s) 707
BseBI CCWGG 2 cut(s) 353, 404
BseCI ATCGAT 1 cut(s) 426
BseDI CCNNGG 3 cut(s) 351, 403, 868
BseGI GGATG 5 cut(s) 32, 478, 805, 879, 967
BseLI CCNNNNNNNGG 2 cut(s) 531, 658
BseMI GCAATG 1 cut(s) 707
BseMII CTCAG 1 cut(s) 571
BseRI GAGGAG 7 cut(s) 16, 28, 145, 148, 173, 737, 740
BseSI GKGCMC 1 cut(s) 586
BseX3I CGGCCG 1 cut(s) 854
BseXI GCAGC 5 cut(s) 189, 460, 508, 653, 946
Bsh1236I CGCG 1 cut(s) 509
Bsh1285I CGRYCG 1 cut(s) 857
BshFI GGCC 3 cut(s) 251, 683, 856
BshVI ATCGAT 1 cut(s) 426
BsiEI CGRYCG 1 cut(s) 857
BsiHKCI CYCGRG 1 cut(s) 747
BsiSI CCGG 2 cut(s) 853, 931
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 2 cut(s) 531, 658
BsmAI GTCTC 1 cut(s) 744
BsmBI CGTCTC 1 cut(s) 744
BsmFI GGGAC 1 cut(s) 116
BsmI GAATGC 1 cut(s) 904
BsnI GGCC 3 cut(s) 251, 683, 856
BsoBI CYCGRG 1 cut(s) 747
Bsp119I TTCGAA 1 cut(s) 410
Bsp1286I GDGCHC 1 cut(s) 586
Bsp143I GATC 2 cut(s) 489, 892
BspANI GGCC 3 cut(s) 251, 683, 856
BspCNI CTCAG 1 cut(s) 570
BspDI ATCGAT 1 cut(s) 426
BspFNI CGCG 1 cut(s) 509
BspHI TCATGA 1 cut(s) 540
BspLI GGNNCC 1 cut(s) 840
BspMAI CTGCAG 3 cut(s) 450, 498, 789
BspMI ACCTGC 2 cut(s) 703, 763
BspPI GGATC 1 cut(s) 484
BspT104I TTCGAA 1 cut(s) 410
BsrDI GCAATG 1 cut(s) 707
BsrFI RCCGGY 1 cut(s) 930
BssAI RCCGGY 1 cut(s) 930
BssECI CCNNGG 3 cut(s) 351, 403, 868
BssMI GATC 2 cut(s) 489, 892
Bst2UI CCWGG 2 cut(s) 353, 404
Bst4CI ACNGT 1 cut(s) 884
Bst6I CTCTTC 1 cut(s) 34
BstAPI GCANNNNNTGC 1 cut(s) 940
BstBI TTCGAA 1 cut(s) 410
BstC8I GCNNGC 3 cut(s) 306, 327, 586
BstDEI CTNAG 1 cut(s) 557
BstDSI CCRYGG 1 cut(s) 868
BstF5I GGATG 5 cut(s) 32, 478, 805, 879, 967
BstFNI CGCG 1 cut(s) 509
BstH2I RGCGCY 3 cut(s) 347, 485, 502
BstHHI GCGC 5 cut(s) 346, 484, 501, 514, 772
BstKTI GATC 2 cut(s) 492, 895
BstMAI GTCTC 1 cut(s) 744
BstMBI GATC 2 cut(s) 489, 892
BstMCI CGRYCG 1 cut(s) 857
BstMWI GCNNNNNNNGC 8 cut(s) 248, 428, 448, 454, 567, 675, 743, 940
BstNI CCWGG 2 cut(s) 353, 404
BstPAI GACNNNNGTC 1 cut(s) 353
BstSCI CCNGG 2 cut(s) 351, 402
BstSFI CTRYAG 3 cut(s) 446, 494, 785
BstSLI GKGCMC 1 cut(s) 586
BstUI CGCG 1 cut(s) 509
BstV1I GCAGC 5 cut(s) 189, 460, 508, 653, 946
BstX2I RGATCY 1 cut(s) 489
BstYI RGATCY 1 cut(s) 489
BstZI CGGCCG 1 cut(s) 854
Bsu15I ATCGAT 1 cut(s) 426
BsuRI GGCC 3 cut(s) 251, 683, 856
BsuTUI ATCGAT 1 cut(s) 426
BtgI CCRYGG 1 cut(s) 868
BtgZI GCGATG 1 cut(s) 369
BtsCI GGATG 5 cut(s) 32, 478, 805, 879, 967
BveI ACCTGC 2 cut(s) 703, 763
Cac8I GCNNGC 3 cut(s) 306, 327, 586
CaiI CAGNNNCTG 1 cut(s) 784
CciI TCATGA 1 cut(s) 540
CfoI GCGC 5 cut(s) 346, 484, 501, 514, 772
Cfr10I RCCGGY 1 cut(s) 930
Cfr13I GGNCC 2 cut(s) 661, 838
ClaI ATCGAT 1 cut(s) 426
CseI GACGC 1 cut(s) 812
Csp6I GTAC 2 cut(s) 859, 928
CviAII CATG 1 cut(s) 541
CviQI GTAC 2 cut(s) 859, 928
DdeI CTNAG 1 cut(s) 557
DpnI GATC 2 cut(s) 491, 894
DpnII GATC 2 cut(s) 489, 892
DraIII CACNNNGTG 1 cut(s) 531
EaeI YGGCCR 2 cut(s) 249, 854
EagI CGGCCG 1 cut(s) 854
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
EciI GGCGGA 2 cut(s) 151, 312
EclXI CGGCCG 1 cut(s) 854
Eco47I GGWCC 2 cut(s) 661, 838
Eco47III AGCGCT 1 cut(s) 500
Eco52I CGGCCG 1 cut(s) 854
Eco88I CYCGRG 1 cut(s) 747
EcoO109I RGGNCCY 1 cut(s) 838
EcoRI GAATTC 1 cut(s) 412
EcoRII CCWGG 2 cut(s) 351, 402
Esp3I CGTCTC 1 cut(s) 744
FaeI CATG 1 cut(s) 544
FaiI YATR 1 cut(s) 542
FaqI GGGAC 1 cut(s) 116
FatI CATG 1 cut(s) 540
FauI CCCGC 1 cut(s) 593
Fnu4HI GCNGC 9 cut(s) 178, 249, 273, 449, 497, 510, 667, 670, 935
FokI GGATG 5 cut(s) 19, 485, 812, 886, 974
Fsp4HI GCNGC 9 cut(s) 178, 249, 273, 449, 497, 510, 667, 670, 935
FspBI CTAG 2 cut(s) 309, 549
GlaI GCGC 5 cut(s) 345, 483, 500, 513, 771
GluI GCNGC 9 cut(s) 178, 249, 273, 449, 497, 510, 667, 670, 935
HaeII RGCGCY 3 cut(s) 347, 485, 502
HaeIII GGCC 3 cut(s) 251, 683, 856
HapII CCGG 2 cut(s) 853, 931
HgaI GACGC 1 cut(s) 812
HhaI GCGC 5 cut(s) 346, 484, 501, 514, 772
Hin1II CATG 1 cut(s) 544
Hin6I GCGC 5 cut(s) 344, 482, 499, 512, 770
HinP1I GCGC 5 cut(s) 344, 482, 499, 512, 770
HinfI GANTC 4 cut(s) 217, 264, 704, 990
HpaII CCGG 2 cut(s) 853, 931
HphI GGTGA 2 cut(s) 259, 274
Hpy166II GTNNAC 1 cut(s) 282
Hpy188I TCNGA 4 cut(s) 263, 397, 418, 897
Hpy188III TCNNGA 6 cut(s) 487, 541, 643, 749, 987, 1003
Hpy8I GTNNAC 1 cut(s) 282
HpyAV CCTTC 3 cut(s) 71, 668, 733
HpyCH4III ACNGT 1 cut(s) 884
HpyCH4IV ACGT 1 cut(s) 753
HpyCH4V TGCA 5 cut(s) 329, 448, 496, 712, 787
HpyF10VI GCNNNNNNNGC 8 cut(s) 248, 428, 448, 454, 567, 675, 743, 940
HpyF3I CTNAG 1 cut(s) 557
HpySE526I ACGT 1 cut(s) 753
Hsp92II CATG 1 cut(s) 544
HspAI GCGC 5 cut(s) 344, 482, 499, 512, 770
Kzo9I GATC 2 cut(s) 489, 892
LmnI GCTCC 1 cut(s) 913
Lsp1109I GCAGC 5 cut(s) 189, 460, 508, 653, 946
LweI GCATC 1 cut(s) 418
MaeI CTAG 2 cut(s) 309, 549
MaeII ACGT 1 cut(s) 753
MaeIII GTNAC 4 cut(s) 145, 265, 355, 1007
MalI GATC 2 cut(s) 491, 894
MboI GATC 2 cut(s) 489, 892
MboII GAAGA 6 cut(s) 21, 37, 80, 350, 802, 958
MflI RGATCY 1 cut(s) 489
MhlI GDGCHC 1 cut(s) 586
MluCI AATT 7 cut(s) 109, 118, 412, 536, 758, 824, 1015
MlyI GAGTC 2 cut(s) 273, 999
MmeI TCCRAC 1 cut(s) 420
MroXI GAANNNNTTC 2 cut(s) 848, 950
MseI TTAA 1 cut(s) 756
MspA1I CMGCKG 1 cut(s) 666
MspI CCGG 2 cut(s) 853, 931
MspR9I CCNGG 2 cut(s) 353, 404
Mva1269I GAATGC 1 cut(s) 904
MvaI CCWGG 2 cut(s) 353, 404
MvnI CGCG 1 cut(s) 509
MwoI GCNNNNNNNGC 8 cut(s) 248, 428, 448, 454, 567, 675, 743, 940
NdeII GATC 2 cut(s) 489, 892
NlaIII CATG 1 cut(s) 544
NlaIV GGNNCC 1 cut(s) 840
NmuCI GTSAC 4 cut(s) 145, 265, 355, 1007
NspV TTCGAA 1 cut(s) 410
PaeR7I CTCGAG 1 cut(s) 747
PagI TCATGA 1 cut(s) 540
PcsI WCGNNNNNNNCGW 3 cut(s) 312, 465, 1008
PctI GAATGC 1 cut(s) 904
PdmI GAANNNNTTC 2 cut(s) 848, 950
PfeI GAWTC 2 cut(s) 217, 704
PflMI CCANNNNNTGG 1 cut(s) 531
PkrI GCNGC 9 cut(s) 179, 250, 274, 450, 498, 511, 668, 671, 936
PleI GAGTC 2 cut(s) 272, 998
PpsI GAGTC 2 cut(s) 272, 998
PpuMI RGGWCCY 1 cut(s) 838
PshAI GACNNNNGTC 1 cut(s) 353
Psp5II RGGWCCY 1 cut(s) 838
Psp6I CCWGG 2 cut(s) 351, 402
PspGI CCWGG 2 cut(s) 351, 402
PspN4I GGNNCC 1 cut(s) 840
PspPI GGNCC 2 cut(s) 661, 838
PspPPI RGGWCCY 1 cut(s) 838
PstI CTGCAG 3 cut(s) 450, 498, 789
PstNI CAGNNNCTG 1 cut(s) 784
PsuI RGATCY 1 cut(s) 489
RsaI GTAC 2 cut(s) 860, 929
RsaNI GTAC 2 cut(s) 859, 928
SaqAI TTAA 1 cut(s) 756
SatI GCNGC 9 cut(s) 178, 249, 273, 449, 497, 510, 667, 670, 935
Sau3AI GATC 2 cut(s) 489, 892
Sau96I GGNCC 2 cut(s) 661, 838
SchI GAGTC 2 cut(s) 273, 999
ScrFI CCNGG 2 cut(s) 353, 404
SduI GDGCHC 1 cut(s) 586
SfaNI GCATC 1 cut(s) 418
SfcI CTRYAG 3 cut(s) 446, 494, 785
Sfr274I CTCGAG 1 cut(s) 747
SfuI TTCGAA 1 cut(s) 410
SinI GGWCC 2 cut(s) 661, 838
SlaI CTCGAG 1 cut(s) 747
SmlI CTYRAG 3 cut(s) 485, 747, 970
SmoI CTYRAG 3 cut(s) 485, 747, 970
Sse9I AATT 7 cut(s) 109, 118, 412, 536, 758, 824, 1015
SspMI CTAG 2 cut(s) 309, 549
StyD4I CCNGG 2 cut(s) 351, 402
TaaI ACNGT 1 cut(s) 884
TaiI ACGT 1 cut(s) 756
TaqII GACCGA 1 cut(s) 350
TasI AATT 7 cut(s) 109, 118, 412, 536, 758, 824, 1015
TauI GCSGC 4 cut(s) 251, 275, 512, 672
TfiI GAWTC 2 cut(s) 217, 704
Tru1I TTAA 1 cut(s) 756
Tru9I TTAA 1 cut(s) 756
TseFI GTSAC 4 cut(s) 145, 265, 355, 1007
TseI GCWGC 5 cut(s) 177, 448, 496, 666, 934
Tsp45I GTSAC 4 cut(s) 145, 265, 355, 1007
TspDTI ATGAA 5 cut(s) 17, 21, 529, 696, 837
TspGWI ACGGA 2 cut(s) 83, 926
Van91I CCANNNNNTGG 1 cut(s) 531
VpaK11BI GGWCC 2 cut(s) 661, 838
XapI RAATTY 2 cut(s) 412, 536
XhoI CTCGAG 1 cut(s) 747
XmnI GAANNNNTTC 2 cut(s) 848, 950
XspI CTAG 2 cut(s) 309, 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.