MD16G1008500.v1.1

Tetratricopeptide repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007638
Physical Location & Seq
Reverse (-)
632454 .. 633482
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1008500.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGAACTCCTCCTCCACTCTCGCTTCCTCCTCCTTCTCTACATCCTCATCTCTCTTCCAATACAAACTCATTCCCAGTAAACCAACCCAACCTTCCCTTCTCCGTTTTGACCCTCCCCATTCCCACAATTCCCACAATGTCCCTGCCCTCAAAGTCATAGCCTCCTCCGCCGCCCACCACCACCGCCCCCTCCTCCCAGGTAACACCCACAACAACCCAAGCCCGATTCTTCAAACTCTTAAACAGTACGCCAAAACCGCCATTCTCATCGGAGTCACCGCCGCCGTGTTCACCACCAAGTCATCGACTTTGCTCGCCATAGCCGAACCTCCGCCGACATTGACCGAAGAAGCGCCGACCCAGGTCACAGATAACGAGAACAACCAATCCTCATCGCCATTGTCCGACTTCCTGGGTTCGAATTCGGAAGCCATCAGTGCCCTGAAATCGCTTCTGCAGCAGAAGCTTGAAAACGGCGAGGACGACGAGGCGCTCAAGATCCTGCAGCGCTTGGTCGCGGCGCAGCCCTCCAACACCGAGTGGAAGTTCATGATGGCGAGGCTTCTCAGCGAAATGGGCGAAAATAAAAGCGCCCGCCAAGTGTTCGAGGAAATACTCGCCGCGAACCCCTTGTCCTTCGAGGCGTTGTTCGAGAACGCCCTGCTGATGGACCGCTGTGGCGAAGGTGAGGCCGTGATCAAGCGGTTGGAGGACGCATTGCAGGTTGCGGAAGAGGAGAACAAGGCCAAGGAGGCTCGGGACGTTAAATTGATAATGGCGCAGGTTCAGTTCTTGCAGAAGAATGTGGAGGACGCATTGAAAAGCTACAATGAATTGGTGAAGGAAGACCCGAAAGACTTCCGGCCGTACTTTTGCCGTGGGATGATTTACAGTTTGCTTGATCGGAATGCAGAGGCGACGGAGCAATTTGAGAAGTACCGGCAGCTTTCACCGAAGAAGTTTGAGGTGGATGGGTACTTGAGGAAGCCATTGTCGAGGGTCAAGCTATTCGGGAGTGACGAGAATTGA

Protein Analysis

343

Amino Acids

38.08

Weight (kDa)

5.76

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_19 PF14559 156 - 211 3.7e-08 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 712, 772
AccII CGCG 2 cut(s) 518, 623
AclWI GGATC 1 cut(s) 493
AcoI YGGCCR 1 cut(s) 863
AcsI RAATTY 1 cut(s) 421
AdeI CACNNNGTG 1 cut(s) 540
AfaI GTAC 4 cut(s) 248, 869, 938, 977
AfeI AGCGCT 1 cut(s) 509
AfiI CCNNNNNNNGG 1 cut(s) 667
AgsI TTSAA 3 cut(s) 233, 470, 820
AjnI CCWGG 3 cut(s) 196, 360, 411
AjuI GAANNNNNNNTTGG 4 cut(s) 76, 81, 108, 113
AloI GAACNNNNNNTCC 5 cut(s) 28, 371, 403, 617, 649
AluBI AGCT 4 cut(s) 466, 825, 946, 1006
AluI AGCT 4 cut(s) 466, 825, 946, 1006
AlwI GGATC 1 cut(s) 493
Ama87I CYCGRG 1 cut(s) 756
Aor51HI AGCGCT 1 cut(s) 509
AoxI GGCC 3 cut(s) 690, 744, 863
ApeKI GCWGC 4 cut(s) 457, 505, 523, 943
ApoI RAATTY 1 cut(s) 421
ArsI GACNNNNNNTTYG 2 cut(s) 617, 649
Asp700I GAANNNNTTC 1 cut(s) 857
AspLEI GCGC 6 cut(s) 355, 493, 510, 523, 593, 781
AspS9I GGNCC 1 cut(s) 670
AsuHPI GGTGA 5 cut(s) 268, 283, 698, 850, 942
AsuII TTCGAA 1 cut(s) 419
AvaI CYCGRG 1 cut(s) 756
AvaII GGWCC 1 cut(s) 670
BaeGI GKGCMC 1 cut(s) 442
BbsI GAAGAC 1 cut(s) 852
BbvI GCAGC 4 cut(s) 469, 517, 535, 955
BccI CCATC 4 cut(s) 440, 547, 661, 965
BceAI ACGGC 5 cut(s) 269, 490, 677, 850, 861
BciT130I CCWGG 3 cut(s) 198, 362, 413
BclI TGATCA 1 cut(s) 696
BfmI CTRYAG 2 cut(s) 455, 503
BfoI RGCGCY 4 cut(s) 356, 494, 511, 594
BfuAI ACCTGC 2 cut(s) 712, 772
BglI GCCNNNNNGGC 1 cut(s) 752
BisI GCNGC 8 cut(s) 171, 282, 458, 506, 519, 524, 621, 944
BlsI GCNGC 8 cut(s) 172, 283, 459, 507, 520, 525, 622, 945
Bme1390I CCNGG 3 cut(s) 198, 362, 413
Bme18I GGWCC 1 cut(s) 670
BmeT110I CYCGRG 1 cut(s) 756
BmgT120I GGNCC 1 cut(s) 670
BmrFI CCNGG 3 cut(s) 198, 362, 413
BmrI ACTGGG 1 cut(s) 69
BmuI ACTGGG 1 cut(s) 69
BoxI GACNNNNGTC 1 cut(s) 362
BpiI GAAGAC 1 cut(s) 852
Bpu14I TTCGAA 1 cut(s) 419
BpuEI CTTGAG 2 cut(s) 479, 1000
BsaJI CCNNGG 5 cut(s) 196, 360, 412, 747, 877
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 667
Bse118I RCCGGY 1 cut(s) 939
Bse1I ACTGG 1 cut(s) 75
Bse3DI GCAATG 1 cut(s) 716
BseBI CCWGG 3 cut(s) 198, 362, 413
BseDI CCNNGG 5 cut(s) 196, 360, 412, 747, 877
BseGI GGATG 3 cut(s) 41, 888, 976
BseLI CCNNNNNNNGG 1 cut(s) 667
BseMI GCAATG 1 cut(s) 716
BseMII CTCAG 1 cut(s) 580
BseNI ACTGG 1 cut(s) 75
BseRI GAGGAG 4 cut(s) 19, 154, 182, 749
BseSI GKGCMC 1 cut(s) 442
BseX3I CGGCCG 1 cut(s) 863
BseXI GCAGC 4 cut(s) 469, 517, 535, 955
Bsh1236I CGCG 2 cut(s) 518, 623
Bsh1285I CGRYCG 1 cut(s) 866
BshFI GGCC 3 cut(s) 692, 746, 865
BsiEI CGRYCG 1 cut(s) 866
BsiHKCI CYCGRG 1 cut(s) 756
BsiSI CCGG 2 cut(s) 862, 940
BslFI GGGAC 2 cut(s) 125, 773
BslI CCNNNNNNNGG 1 cut(s) 667
BsmFI GGGAC 2 cut(s) 125, 773
BsmI GAATGC 1 cut(s) 913
BsnI GGCC 3 cut(s) 692, 746, 865
BsoBI CYCGRG 1 cut(s) 756
Bsp119I TTCGAA 1 cut(s) 419
Bsp1286I GDGCHC 1 cut(s) 442
Bsp143I GATC 3 cut(s) 498, 696, 901
BspANI GGCC 3 cut(s) 692, 746, 865
BspCNI CTCAG 1 cut(s) 579
BspFNI CGCG 2 cut(s) 518, 623
BspHI TCATGA 1 cut(s) 549
BspMAI CTGCAG 2 cut(s) 459, 507
BspMI ACCTGC 2 cut(s) 712, 772
BspPI GGATC 1 cut(s) 493
BspT104I TTCGAA 1 cut(s) 419
BsrDI GCAATG 1 cut(s) 716
BsrFI RCCGGY 1 cut(s) 939
BsrI ACTGG 1 cut(s) 75
BssAI RCCGGY 1 cut(s) 939
BssECI CCNNGG 5 cut(s) 196, 360, 412, 747, 877
BssMI GATC 3 cut(s) 498, 696, 901
BssT1I CCWWGG 1 cut(s) 747
Bst2UI CCWGG 3 cut(s) 198, 362, 413
Bst4CI ACNGT 2 cut(s) 246, 893
Bst6I CTCTTC 2 cut(s) 59, 726
BstBI TTCGAA 1 cut(s) 419
BstC8I GCNNGC 2 cut(s) 315, 595
BstDEI CTNAG 1 cut(s) 566
BstDSI CCRYGG 1 cut(s) 877
BstF5I GGATG 3 cut(s) 41, 888, 976
BstFNI CGCG 2 cut(s) 518, 623
BstH2I RGCGCY 4 cut(s) 356, 494, 511, 594
BstHHI GCGC 6 cut(s) 355, 493, 510, 523, 593, 781
BstKTI GATC 3 cut(s) 501, 699, 904
BstMBI GATC 3 cut(s) 498, 696, 901
BstMCI CGRYCG 1 cut(s) 866
BstMWI GCNNNNNNNGC 7 cut(s) 167, 257, 437, 457, 463, 576, 752
BstNI CCWGG 3 cut(s) 198, 362, 413
BstPAI GACNNNNGTC 1 cut(s) 362
BstSCI CCNGG 3 cut(s) 196, 360, 411
BstSFI CTRYAG 2 cut(s) 455, 503
BstSLI GKGCMC 1 cut(s) 442
BstUI CGCG 2 cut(s) 518, 623
BstV1I GCAGC 4 cut(s) 469, 517, 535, 955
BstV2I GAAGAC 1 cut(s) 852
BstX2I RGATCY 1 cut(s) 498
BstYI RGATCY 1 cut(s) 498
BstZI CGGCCG 1 cut(s) 863
BsuRI GGCC 3 cut(s) 692, 746, 865
BtgI CCRYGG 1 cut(s) 877
BtgZI GCGATG 1 cut(s) 378
BtsCI GGATG 3 cut(s) 41, 888, 976
BtsIMutI CAGTG 1 cut(s) 442
BveI ACCTGC 2 cut(s) 712, 772
Cac8I GCNNGC 2 cut(s) 315, 595
CciI TCATGA 1 cut(s) 549
CfoI GCGC 6 cut(s) 355, 493, 510, 523, 593, 781
Cfr10I RCCGGY 1 cut(s) 939
Cfr13I GGNCC 1 cut(s) 670
CseI GACGC 2 cut(s) 722, 821
Csp6I GTAC 4 cut(s) 247, 868, 937, 976
CviAII CATG 1 cut(s) 550
CviQI GTAC 4 cut(s) 247, 868, 937, 976
DdeI CTNAG 1 cut(s) 566
DpnI GATC 3 cut(s) 500, 698, 903
DpnII GATC 3 cut(s) 498, 696, 901
DraIII CACNNNGTG 1 cut(s) 540
EaeI YGGCCR 1 cut(s) 863
EagI CGGCCG 1 cut(s) 863
Eam1104I CTCTTC 2 cut(s) 59, 726
EarI CTCTTC 2 cut(s) 59, 726
EciI GGCGGA 2 cut(s) 157, 321
EclXI CGGCCG 1 cut(s) 863
Eco130I CCWWGG 1 cut(s) 747
Eco47I GGWCC 1 cut(s) 670
Eco47III AGCGCT 1 cut(s) 509
Eco52I CGGCCG 1 cut(s) 863
Eco88I CYCGRG 1 cut(s) 756
EcoRI GAATTC 1 cut(s) 421
EcoRII CCWGG 3 cut(s) 196, 360, 411
EcoT14I CCWWGG 1 cut(s) 747
ErhI CCWWGG 1 cut(s) 747
FaeI CATG 1 cut(s) 553
FaiI YATR 3 cut(s) 158, 320, 551
FaqI GGGAC 2 cut(s) 125, 773
FatI CATG 1 cut(s) 549
FauI CCCGC 1 cut(s) 602
FbaI TGATCA 1 cut(s) 696
Fnu4HI GCNGC 8 cut(s) 171, 282, 458, 506, 519, 524, 621, 944
FokI GGATG 3 cut(s) 28, 895, 983
Fsp4HI GCNGC 8 cut(s) 171, 282, 458, 506, 519, 524, 621, 944
GlaI GCGC 6 cut(s) 354, 492, 509, 522, 592, 780
GluI GCNGC 8 cut(s) 171, 282, 458, 506, 519, 524, 621, 944
HaeII RGCGCY 4 cut(s) 356, 494, 511, 594
HaeIII GGCC 3 cut(s) 692, 746, 865
HapII CCGG 2 cut(s) 862, 940
HgaI GACGC 2 cut(s) 722, 821
HhaI GCGC 6 cut(s) 355, 493, 510, 523, 593, 781
Hin1II CATG 1 cut(s) 553
Hin6I GCGC 6 cut(s) 353, 491, 508, 521, 591, 779
HinP1I GCGC 6 cut(s) 353, 491, 508, 521, 591, 779
HindIII AAGCTT 1 cut(s) 464
HinfI GANTC 2 cut(s) 226, 273
HpaII CCGG 2 cut(s) 862, 940
HphI GGTGA 5 cut(s) 268, 283, 698, 850, 942
Hpy166II GTNNAC 2 cut(s) 80, 291
Hpy188I TCNGA 4 cut(s) 272, 406, 427, 906
Hpy188III TCNNGA 5 cut(s) 496, 550, 652, 758, 1012
Hpy8I GTNNAC 2 cut(s) 80, 291
Hpy99I CGWCG 2 cut(s) 488, 922
HpyAV CCTTC 6 cut(s) 43, 102, 107, 646, 677, 835
HpyCH4III ACNGT 2 cut(s) 246, 893
HpyCH4IV ACGT 1 cut(s) 762
HpyCH4V TGCA 5 cut(s) 457, 505, 721, 796, 911
HpyF10VI GCNNNNNNNGC 7 cut(s) 167, 257, 437, 457, 463, 576, 752
HpyF3I CTNAG 1 cut(s) 566
HpySE526I ACGT 1 cut(s) 762
Hsp92II CATG 1 cut(s) 553
HspAI GCGC 6 cut(s) 353, 491, 508, 521, 591, 779
Ksp22I TGATCA 1 cut(s) 696
Kzo9I GATC 3 cut(s) 498, 696, 901
LmnI GCTCC 1 cut(s) 922
Lsp1109I GCAGC 4 cut(s) 469, 517, 535, 955
MaeII ACGT 1 cut(s) 762
MaeIII GTNAC 4 cut(s) 200, 274, 364, 1016
MalI GATC 3 cut(s) 500, 698, 903
MboI GATC 3 cut(s) 498, 696, 901
MboII GAAGA 7 cut(s) 46, 221, 359, 743, 811, 857, 967
MflI RGATCY 1 cut(s) 498
MhlI GDGCHC 1 cut(s) 442
MluCI AATT 6 cut(s) 127, 421, 767, 833, 926, 1024
MlyI GAGTC 1 cut(s) 282
MmeI TCCRAC 3 cut(s) 429, 555, 687
MroXI GAANNNNTTC 1 cut(s) 857
MseI TTAA 2 cut(s) 240, 765
MspA1I CMGCKG 1 cut(s) 675
MspI CCGG 2 cut(s) 862, 940
MspR9I CCNGG 3 cut(s) 198, 362, 413
Mva1269I GAATGC 1 cut(s) 913
MvaI CCWGG 3 cut(s) 198, 362, 413
MvnI CGCG 2 cut(s) 518, 623
MwoI GCNNNNNNNGC 7 cut(s) 167, 257, 437, 457, 463, 576, 752
NdeII GATC 3 cut(s) 498, 696, 901
NlaIII CATG 1 cut(s) 553
NmuCI GTSAC 3 cut(s) 274, 364, 1016
NspV TTCGAA 1 cut(s) 419
PagI TCATGA 1 cut(s) 549
PcsI WCGNNNNNNNCGW 3 cut(s) 321, 480, 1017
PctI GAATGC 1 cut(s) 913
PdmI GAANNNNTTC 1 cut(s) 857
PfeI GAWTC 1 cut(s) 226
PkrI GCNGC 8 cut(s) 172, 283, 459, 507, 520, 525, 622, 945
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
PshAI GACNNNNGTC 1 cut(s) 362
Psp6I CCWGG 3 cut(s) 196, 360, 411
PspGI CCWGG 3 cut(s) 196, 360, 411
PspPI GGNCC 1 cut(s) 670
PstI CTGCAG 2 cut(s) 459, 507
PsuI RGATCY 1 cut(s) 498
RsaI GTAC 4 cut(s) 248, 869, 938, 977
RsaNI GTAC 4 cut(s) 247, 868, 937, 976
SaqAI TTAA 2 cut(s) 240, 765
SatI GCNGC 8 cut(s) 171, 282, 458, 506, 519, 524, 621, 944
Sau3AI GATC 3 cut(s) 498, 696, 901
Sau96I GGNCC 1 cut(s) 670
SchI GAGTC 1 cut(s) 282
ScrFI CCNGG 3 cut(s) 198, 362, 413
SduI GDGCHC 1 cut(s) 442
SfcI CTRYAG 2 cut(s) 455, 503
SfuI TTCGAA 1 cut(s) 419
SinI GGWCC 1 cut(s) 670
SmlI CTYRAG 2 cut(s) 494, 979
SmoI CTYRAG 2 cut(s) 494, 979
Sse9I AATT 6 cut(s) 127, 421, 767, 833, 926, 1024
StyD4I CCNGG 3 cut(s) 196, 360, 411
StyI CCWWGG 1 cut(s) 747
TaaI ACNGT 2 cut(s) 246, 893
TaiI ACGT 1 cut(s) 765
TaqI TCGA 6 cut(s) 305, 419, 606, 639, 651, 995
TaqII GACCGA 1 cut(s) 359
TasI AATT 6 cut(s) 127, 421, 767, 833, 926, 1024
TauI GCSGC 4 cut(s) 173, 284, 521, 623
TfiI GAWTC 1 cut(s) 226
Tru1I TTAA 2 cut(s) 240, 765
Tru9I TTAA 2 cut(s) 240, 765
TscAI CASTG 1 cut(s) 442
TseFI GTSAC 3 cut(s) 274, 364, 1016
TseI GCWGC 4 cut(s) 457, 505, 523, 943
Tsp45I GTSAC 3 cut(s) 274, 364, 1016
TspDTI ATGAA 3 cut(s) 17, 538, 846
TspGWI ACGGA 2 cut(s) 92, 935
TspRI CASTG 1 cut(s) 442
VpaK11BI GGWCC 1 cut(s) 670
XapI RAATTY 1 cut(s) 421
XmnI GAANNNNTTC 1 cut(s) 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.