MD13G1011000.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006076
Physical Location & Seq
Reverse (-)
666151 .. 668317
2167 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1011000.v1.1.491

Sequence Viewer

Length: 984 bp
ATGAGGATGTCTTTCCCCTTCTTTTCCGGCCATGGAAATGTCGCAGCTGTTGCACTCGTGATGTTGGTTTTCATGAGCACAACAAGCGAAGCCCAATTATCTTCTACATTTTATGACAAAACCTGTCCAACCGCTCTTACCACTATAAAAAATGCCATAAGAACAGCTGTTTCCAGTGAAAGGCGGATGGCCGCGTCCCTAATCCGCCTCCACTTCCATGATTGCTTTGTCCAGGGTTGTGACGCATCAATCTTACTTGATGACGCTCCTTCTATCACAAGTGAAAAGGGCGCATTTCAAAACAACGGTTCTGTGAGAGGCTTTGAAGTCATAGATAAAGCAAAAGCTGAAGTAGAGAAGGTCTGCCCTGGTGTCGTATCTTGCGCAGATATTCTCGCTGTAGCCGCTCGGGATGCATCCGTTGCCGTGAGCGGTCCATCTTGGACGGTGAAACTTGGAAGAAGAGATTCCACCACAGCAAGCCAAAACCTAGCTGAAAGTTCACTTCCACTTTTCACAGACAGCCTTGACAGCCTGAGAGAGCGATTTGGCGACTTAGGCCTCAACGCGAGAGATTTGGTTGCACTGTCAGGTGCACATACAATTGGACAGGCTCGTTGCGTTACATTCCGTGGCAGGATTTACAATGCAAGTGACATCGATTCTAACTTTGCCACTACTCGCAAACGTCGTTGTCCAGCTGATGCAAACAACGGCGATGCAAATTTGGCACCGCTCGAGTTGGTGACACCGAACTCATTTGACAACAATTACTACAAGAATTTGATTCAGAAGAAGGGTCTTCTTGAATCGGATCAAGTTCTTTTTAGTGGAGGTTCCACAGACAGCATCGTCTCCGAGTACAGTTCCAAGCCCGCAACTTTTAAGGCTGATTTTATCACCGCTATGATTAAAATGGGAGACATTAATCCTCTCACCGGTTCAGCTGGAGAGGTCAGAAGGATTTGCAGTGCTCTCAACTAA

Protein Analysis

328

Amino Acids

35.07

Weight (kDa)

6.89

Isoelectric Point (pI)

35.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 50 - 290 4e-84 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 851
Acc16I TGCGCA 1 cut(s) 385
AccB1I GGYRCC 1 cut(s) 730
AccBSI CCGCTC 4 cut(s) 134, 407, 432, 736
AccII CGCG 2 cut(s) 194, 569
AciI CCGC 9 cut(s) 132, 184, 192, 205, 405, 432, 734, 876, 903
AclWI GGATC 1 cut(s) 822
AcoI YGGCCR 2 cut(s) 28, 189
AcsI RAATTY 2 cut(s) 724, 781
AcuI CTGAAG 1 cut(s) 369
AfaI GTAC 1 cut(s) 863
AfiI CCNNNNNNNGG 2 cut(s) 180, 938
AgeI ACCGGT 1 cut(s) 938
AgsI TTSAA 3 cut(s) 299, 326, 809
AjnI CCWGG 2 cut(s) 231, 367
AluBI AGCT 6 cut(s) 47, 167, 347, 494, 701, 947
AluI AGCT 6 cut(s) 47, 167, 347, 494, 701, 947
Alw21I GWGCWC 3 cut(s) 80, 598, 976
Alw26I GTCTC 2 cut(s) 859, 915
Alw44I GTGCAC 1 cut(s) 594
AlwI GGATC 1 cut(s) 822
Ama87I CYCGRG 2 cut(s) 408, 737
AoxI GGCC 3 cut(s) 28, 189, 559
ApaLI GTGCAC 1 cut(s) 594
ApeKI GCWGC 1 cut(s) 44
ApoI RAATTY 2 cut(s) 724, 781
AseI ATTAAT 1 cut(s) 927
AsiGI ACCGGT 1 cut(s) 938
Asp700I GAANNNNTTC 1 cut(s) 466
AspLEI GCGC 2 cut(s) 293, 386
AspS9I GGNCC 1 cut(s) 434
AsuHPI GGTGA 4 cut(s) 460, 757, 892, 928
AvaI CYCGRG 2 cut(s) 408, 737
AvaII GGWCC 1 cut(s) 434
BaeGI GKGCMC 1 cut(s) 598
BanI GGYRCC 1 cut(s) 730
BauI CACGAG 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 794
Bbv12I GWGCWC 3 cut(s) 80, 598, 976
BbvI GCAGC 1 cut(s) 56
BccI CCATC 2 cut(s) 181, 445
BceAI ACGGC 2 cut(s) 410, 730
BciT130I CCWGG 2 cut(s) 233, 369
BcoDI GTCTC 2 cut(s) 859, 915
BfaI CTAG 1 cut(s) 491
BfmI CTRYAG 1 cut(s) 399
BisI GCNGC 3 cut(s) 45, 192, 405
BlsI GCNGC 3 cut(s) 46, 193, 406
Bme1390I CCNGG 2 cut(s) 233, 369
Bme18I GGWCC 1 cut(s) 434
BmeT110I CYCGRG 2 cut(s) 408, 737
BmgT120I GGNCC 1 cut(s) 434
BmiI GGNNCC 2 cut(s) 732, 838
BmrFI CCNGG 2 cut(s) 233, 369
BmsI GCATC 6 cut(s) 254, 403, 425, 694, 709, 858
BpiI GAAGAC 1 cut(s) 794
BpmI CTGGAG 1 cut(s) 969
Bsa29I ATCGAT 1 cut(s) 660
BsaJI CCNNGG 4 cut(s) 31, 232, 367, 631
BsaWI WCCGGW 1 cut(s) 938
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 938
Bse118I RCCGGY 1 cut(s) 938
Bse1I ACTGG 1 cut(s) 174
BseBI CCWGG 2 cut(s) 233, 369
BseCI ATCGAT 1 cut(s) 660
BseDI CCNNGG 4 cut(s) 31, 232, 367, 631
BseGI GGATG 4 cut(s) 12, 192, 416, 418
BseLI CCNNNNNNNGG 2 cut(s) 180, 938
BseMII CTCAG 1 cut(s) 527
BseNI ACTGG 1 cut(s) 174
BseSI GKGCMC 1 cut(s) 598
BseXI GCAGC 1 cut(s) 56
Bsh1236I CGCG 2 cut(s) 194, 569
BshFI GGCC 3 cut(s) 30, 191, 561
BshNI GGYRCC 1 cut(s) 730
BshTI ACCGGT 1 cut(s) 938
BshVI ATCGAT 1 cut(s) 660
BsiHKAI GWGCWC 3 cut(s) 80, 598, 976
BsiHKCI CYCGRG 2 cut(s) 408, 737
BsiSI CCGG 2 cut(s) 27, 939
BslFI GGGAC 1 cut(s) 181
BslI CCNNNNNNNGG 2 cut(s) 180, 938
BsmAI GTCTC 2 cut(s) 859, 915
BsmBI CGTCTC 1 cut(s) 859
BsmFI GGGAC 1 cut(s) 181
BsnI GGCC 3 cut(s) 30, 191, 561
BsoBI CYCGRG 2 cut(s) 408, 737
Bsp1286I GDGCHC 3 cut(s) 80, 598, 976
Bsp143I GATC 1 cut(s) 814
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 9 cut(s) 132, 184, 192, 205, 405, 432, 734, 876, 903
BspANI GGCC 3 cut(s) 30, 191, 561
BspCNI CTCAG 1 cut(s) 528
BspDI ATCGAT 1 cut(s) 660
BspFNI CGCG 2 cut(s) 194, 569
BspHI TCATGA 1 cut(s) 72
BspLI GGNNCC 2 cut(s) 732, 838
BspPI GGATC 1 cut(s) 822
BspT107I GGYRCC 1 cut(s) 730
BsrBI CCGCTC 4 cut(s) 134, 407, 432, 736
BsrFI RCCGGY 1 cut(s) 938
BsrI ACTGG 1 cut(s) 174
BssAI RCCGGY 1 cut(s) 938
BssECI CCNNGG 4 cut(s) 31, 232, 367, 631
BssMI GATC 1 cut(s) 814
BssSI CACGAG 1 cut(s) 56
BssT1I CCWWGG 1 cut(s) 31
Bst2BI CACGAG 1 cut(s) 56
Bst2UI CCWGG 2 cut(s) 233, 369
Bst4CI ACNGT 4 cut(s) 308, 448, 588, 866
Bst6I CTCTTC 1 cut(s) 457
BstAPI GCANNNNNTGC 2 cut(s) 50, 422
BstC8I GCNNGC 2 cut(s) 481, 876
BstDEI CTNAG 2 cut(s) 536, 556
BstDSI CCRYGG 2 cut(s) 31, 631
BstF5I GGATG 4 cut(s) 12, 192, 416, 418
BstFNI CGCG 2 cut(s) 194, 569
BstHHI GCGC 2 cut(s) 293, 386
BstKTI GATC 1 cut(s) 817
BstMAI GTCTC 2 cut(s) 859, 915
BstMBI GATC 1 cut(s) 814
BstMWI GCNNNNNNNGC 8 cut(s) 50, 84, 404, 413, 422, 531, 558, 728
BstNI CCWGG 2 cut(s) 233, 369
BstSCI CCNGG 2 cut(s) 231, 367
BstSFI CTRYAG 1 cut(s) 399
BstSLI GKGCMC 1 cut(s) 598
BstUI CGCG 2 cut(s) 194, 569
BstV1I GCAGC 1 cut(s) 56
BstV2I GAAGAC 1 cut(s) 794
Bsu15I ATCGAT 1 cut(s) 660
BsuRI GGCC 3 cut(s) 30, 191, 561
BsuTUI ATCGAT 1 cut(s) 660
BtgI CCRYGG 2 cut(s) 31, 631
BtgZI GCGATG 1 cut(s) 732
BtsCI GGATG 4 cut(s) 12, 192, 416, 418
BtsI GCAGTG 1 cut(s) 976
BtsIMutI CAGTG 3 cut(s) 181, 584, 976
Cac8I GCNNGC 2 cut(s) 481, 876
CciI TCATGA 1 cut(s) 72
CfoI GCGC 2 cut(s) 293, 386
Cfr10I RCCGGY 1 cut(s) 938
Cfr13I GGNCC 1 cut(s) 434
ClaI ATCGAT 1 cut(s) 660
CseI GACGC 3 cut(s) 183, 251, 272
Csp6I GTAC 1 cut(s) 862
CspAI ACCGGT 1 cut(s) 938
CviAII CATG 3 cut(s) 32, 73, 218
CviQI GTAC 1 cut(s) 862
DdeI CTNAG 2 cut(s) 536, 556
DpnI GATC 1 cut(s) 816
DpnII GATC 1 cut(s) 814
DrdI GACNNNNNNGTC 1 cut(s) 851
DseDI GACNNNNNNGTC 1 cut(s) 851
EaeI YGGCCR 2 cut(s) 28, 189
Eam1104I CTCTTC 1 cut(s) 457
EarI CTCTTC 1 cut(s) 457
EciI GGCGGA 2 cut(s) 194, 199
Eco130I CCWWGG 1 cut(s) 31
Eco147I AGGCCT 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 434
Eco57I CTGAAG 1 cut(s) 369
Eco88I CYCGRG 2 cut(s) 408, 737
EcoRII CCWGG 2 cut(s) 231, 367
EcoT14I CCWWGG 1 cut(s) 31
EcoT22I ATGCAT 1 cut(s) 418
ErhI CCWWGG 1 cut(s) 31
Esp3I CGTCTC 1 cut(s) 859
FaeI CATG 3 cut(s) 35, 76, 221
FaiI YATR 9 cut(s) 33, 74, 114, 146, 158, 219, 332, 600, 908
FaqI GGGAC 1 cut(s) 181
FatI CATG 3 cut(s) 31, 72, 217
FauI CCCGC 1 cut(s) 883
Fnu4HI GCNGC 3 cut(s) 45, 192, 405
FokI GGATG 4 cut(s) 19, 199, 403, 425
Fsp4HI GCNGC 3 cut(s) 45, 192, 405
FspBI CTAG 1 cut(s) 491
FspI TGCGCA 1 cut(s) 385
GlaI GCGC 2 cut(s) 292, 385
GluI GCNGC 3 cut(s) 45, 192, 405
GsuI CTGGAG 1 cut(s) 969
HaeIII GGCC 3 cut(s) 30, 191, 561
HapII CCGG 2 cut(s) 27, 939
HgaI GACGC 3 cut(s) 183, 251, 272
HhaI GCGC 2 cut(s) 293, 386
Hin1II CATG 3 cut(s) 35, 76, 221
Hin6I GCGC 2 cut(s) 291, 384
HinP1I GCGC 2 cut(s) 291, 384
HinfI GANTC 4 cut(s) 467, 662, 787, 809
HpaII CCGG 2 cut(s) 27, 939
HphI GGTGA 4 cut(s) 460, 757, 892, 928
Hpy166II GTNNAC 2 cut(s) 503, 596
Hpy188I TCNGA 4 cut(s) 792, 814, 859, 959
Hpy188III TCNNGA 4 cut(s) 58, 73, 410, 806
Hpy8I GTNNAC 2 cut(s) 503, 596
Hpy99I CGWCG 1 cut(s) 693
HpyAV CCTTC 5 cut(s) 28, 279, 352, 790, 954
HpyCH4III ACNGT 4 cut(s) 308, 448, 588, 866
HpyCH4IV ACGT 1 cut(s) 688
HpyCH4V TGCA 8 cut(s) 53, 416, 584, 596, 650, 707, 722, 969
HpyF10VI GCNNNNNNNGC 8 cut(s) 50, 84, 404, 413, 422, 531, 558, 728
HpyF3I CTNAG 2 cut(s) 536, 556
HpySE526I ACGT 1 cut(s) 688
Hsp92II CATG 3 cut(s) 35, 76, 221
HspAI GCGC 2 cut(s) 291, 384
Kzo9I GATC 1 cut(s) 814
LmnI GCTCC 1 cut(s) 271
Lsp1109I GCAGC 1 cut(s) 56
LweI GCATC 6 cut(s) 254, 403, 425, 694, 709, 858
MaeI CTAG 1 cut(s) 491
MaeII ACGT 1 cut(s) 688
MaeIII GTNAC 4 cut(s) 239, 622, 653, 745
MalI GATC 1 cut(s) 816
MbiI CCGCTC 4 cut(s) 134, 407, 432, 736
MboI GATC 1 cut(s) 814
MboII GAAGA 5 cut(s) 93, 471, 474, 794, 805
MfeI CAATTG 1 cut(s) 603
MhlI GDGCHC 3 cut(s) 80, 598, 976
MluCI AATT 5 cut(s) 95, 603, 724, 769, 781
MmeI TCCRAC 1 cut(s) 152
MnlI CCTC 6 cut(s) 218, 311, 572, 827, 942, 946
Mph1103I ATGCAT 1 cut(s) 418
MroXI GAANNNNTTC 1 cut(s) 466
MseI TTAA 3 cut(s) 885, 912, 927
MslI CAYNNNNRTG 3 cut(s) 36, 216, 905
MspA1I CMGCKG 4 cut(s) 47, 167, 701, 947
MspI CCGG 2 cut(s) 27, 939
MspR9I CCNGG 2 cut(s) 233, 369
MunI CAATTG 1 cut(s) 603
MvaI CCWGG 2 cut(s) 233, 369
MvnI CGCG 2 cut(s) 194, 569
MwoI GCNNNNNNNGC 8 cut(s) 50, 84, 404, 413, 422, 531, 558, 728
NcoI CCATGG 1 cut(s) 31
NdeII GATC 1 cut(s) 814
NlaIII CATG 3 cut(s) 35, 76, 221
NlaIV GGNNCC 2 cut(s) 732, 838
NmuCI GTSAC 3 cut(s) 239, 653, 745
NsbI TGCGCA 1 cut(s) 385
NsiI ATGCAT 1 cut(s) 418
PaeR7I CTCGAG 1 cut(s) 737
PagI TCATGA 1 cut(s) 72
PceI AGGCCT 1 cut(s) 561
PcsI WCGNNNNNNNCGW 1 cut(s) 688
PdmI GAANNNNTTC 1 cut(s) 466
PfeI GAWTC 4 cut(s) 467, 662, 787, 809
PinAI ACCGGT 1 cut(s) 938
PkrI GCNGC 3 cut(s) 46, 193, 406
PshBI ATTAAT 1 cut(s) 927
Psp6I CCWGG 2 cut(s) 231, 367
PspGI CCWGG 2 cut(s) 231, 367
PspN4I GGNNCC 2 cut(s) 732, 838
PspPI GGNCC 1 cut(s) 434
PspXI VCTCGAGB 1 cut(s) 737
PvuII CAGCTG 4 cut(s) 47, 167, 701, 947
RsaI GTAC 1 cut(s) 863
RsaNI GTAC 1 cut(s) 862
RseI CAYNNNNRTG 3 cut(s) 36, 216, 905
SaqAI TTAA 3 cut(s) 885, 912, 927
SatI GCNGC 3 cut(s) 45, 192, 405
Sau3AI GATC 1 cut(s) 814
Sau96I GGNCC 1 cut(s) 434
ScrFI CCNGG 2 cut(s) 233, 369
SduI GDGCHC 3 cut(s) 80, 598, 976
SfaNI GCATC 6 cut(s) 254, 403, 425, 694, 709, 858
SfcI CTRYAG 1 cut(s) 399
Sfr274I CTCGAG 1 cut(s) 737
SinI GGWCC 1 cut(s) 434
SlaI CTCGAG 1 cut(s) 737
SmiMI CAYNNNNRTG 3 cut(s) 36, 216, 905
SmlI CTYRAG 1 cut(s) 737
SmoI CTYRAG 1 cut(s) 737
Sse9I AATT 5 cut(s) 95, 603, 724, 769, 781
SseBI AGGCCT 1 cut(s) 561
SsiI CCGC 9 cut(s) 132, 184, 192, 205, 405, 432, 734, 876, 903
SspMI CTAG 1 cut(s) 491
StuI AGGCCT 1 cut(s) 561
StyD4I CCNGG 2 cut(s) 231, 367
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 4 cut(s) 308, 448, 588, 866
TaiI ACGT 1 cut(s) 691
TaqI TCGA 2 cut(s) 660, 738
TasI AATT 5 cut(s) 95, 603, 724, 769, 781
TatI WGTACW 1 cut(s) 861
TauI GCSGC 2 cut(s) 194, 407
TfiI GAWTC 4 cut(s) 467, 662, 787, 809
Tru1I TTAA 3 cut(s) 885, 912, 927
Tru9I TTAA 3 cut(s) 885, 912, 927
TscAI CASTG 3 cut(s) 181, 591, 976
TseFI GTSAC 3 cut(s) 239, 653, 745
TseI GCWGC 1 cut(s) 44
Tsp45I GTSAC 3 cut(s) 239, 653, 745
TspDTI ATGAA 1 cut(s) 61
TspGWI ACGGA 2 cut(s) 409, 620
TspRI CASTG 3 cut(s) 181, 591, 976
VneI GTGCAC 1 cut(s) 594
VpaK11BI GGWCC 1 cut(s) 434
VspI ATTAAT 1 cut(s) 927
XapI RAATTY 2 cut(s) 724, 781
XhoI CTCGAG 1 cut(s) 737
XmnI GAANNNNTTC 1 cut(s) 466
XspI CTAG 1 cut(s) 491
Zsp2I ATGCAT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.