Rh4CG454100

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0006076
Physical Location & Seq
Forward (+)
68682333 .. 68686049
3717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG454100.1

Sequence Viewer

Length: 981 bp
ATGATGAAGTACTTTAGCTCTAATGCAAATGTTGCCTCTGTTGCACTGCTGTTGTTGCTTGCCTTGATGAGCACAACAACCTCTGCTCAGCTCTCCTCGACATTTTATGACAAAGCCTGTCCAAATGCTCTCGCTACTATCAGAAAATCAATAAGAACAGCAGTCTCAGCAGAACGTCGGATGGCAGCCTCCCTAATCCGCCTGCATTTCCATGATTGCTTTGTTCAGGGTTGTGATGCATCAATCTTACTTGACGATACTTCTTCTATCACAGGCGAAAAGAACGCACTGCCTAATCAGAACTCTGTGAGAGGATATGGAGTCATAGATAATGCCAAATCTGAAGTGGAGAAACTATGTCCTGGTGTAGTTTCTTGTGCAGATATCGTCGCCGTGGCTGCTAGGGATGCTTCAGTTGCCGTTGGAGGTCCAGACTGGGCGGTGAAGCTTGGAAGAAGAGATTCGACCACAGCAAGCAAAACTCTAGCTGAAAGCTCGCTTCCCAGTTTCTTAGACAGCCTCGAAAGCCTTGCTAACAGATTTTCTGGTAATGGCCTCAATGTCAGAGATTTGGTTGCACTATCTGGTGCACATTCAATTGGACAAGCTCAGTGCTTTACATTCCGCGGTAGAATCTACAGCAACACCAGTGACATTGATGCAAACTTTGCTCGTACTCGCGGACGTGGTTGTCCAGCAGCAAATAACGTTGGCAATGCAAATTTGGCACCACTTGATTTGGTGACACCTAATCAATTGGACAACAACTACTTCAAGAACTTGATCCAGAAGAAGGGTCTTCTTGCATCTGATCAAGTTCTCTTTAGTGGAGGATCCACAGACAGCATTGTCTCGGAGTATAGCTCAAAACCTGCAAACTTCAAGGCTGATTTTGCCGCCGCCATGATCAAGATGGGAAACATTAACCCTCTCACTGGCTCAAATGGGCAGATAAGAAAGATATGTAGTGCTCTCAACTAA

Protein Analysis

326

Amino Acids

34.49

Weight (kDa)

8.89

Isoelectric Point (pI)

23.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 47 - 289 9.9e-81 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 690, 848
Acc36I ACCTGC 1 cut(s) 880
AccB1I GGYRCC 1 cut(s) 727
AccII CGCG 2 cut(s) 627, 681
AciI CCGC 7 cut(s) 199, 440, 625, 627, 681, 897, 900
AclI AACGTT 1 cut(s) 708
AclWI GGATC 3 cut(s) 778, 828, 841
AcsI RAATTY 1 cut(s) 721
AcuI CTGAAG 2 cut(s) 363, 396
AfaI GTAC 2 cut(s) 11, 676
AfiI CCNNNNNNNGG 2 cut(s) 793, 935
AgsI TTSAA 3 cut(s) 597, 775, 883
AjiI CACGTC 1 cut(s) 686
AjnI CCWGG 1 cut(s) 361
AluBI AGCT 7 cut(s) 18, 91, 448, 488, 495, 608, 864
AluI AGCT 7 cut(s) 18, 91, 448, 488, 495, 608, 864
Alw21I GWGCWC 3 cut(s) 74, 592, 973
Alw26I GTCTC 2 cut(s) 169, 856
Alw44I GTGCAC 1 cut(s) 588
AlwI GGATC 3 cut(s) 778, 828, 841
AoxI GGCC 1 cut(s) 553
ApaLI GTGCAC 1 cut(s) 588
ApeKI GCWGC 3 cut(s) 185, 398, 698
ApoI RAATTY 1 cut(s) 721
Asp700I GAANNNNTTC 1 cut(s) 460
AspS9I GGNCC 1 cut(s) 428
AsuHPI GGTGA 2 cut(s) 454, 754
AvaII GGWCC 1 cut(s) 428
BaeGI GKGCMC 1 cut(s) 592
BamHI GGATCC 1 cut(s) 833
BanI GGYRCC 1 cut(s) 727
BbsI GAAGAC 1 cut(s) 791
Bbv12I GWGCWC 3 cut(s) 74, 592, 973
BbvI GCAGC 3 cut(s) 197, 385, 710
BccI CCATC 2 cut(s) 175, 907
BceAI ACGGC 2 cut(s) 377, 404
BcgI CGANNNNNNTGC 2 cut(s) 512, 546
BciT130I CCWGG 1 cut(s) 363
BclI TGATCA 2 cut(s) 811, 906
BcoDI GTCTC 2 cut(s) 169, 856
BfaI CTAG 2 cut(s) 402, 485
BfmI CTRYAG 1 cut(s) 637
BfuAI ACCTGC 1 cut(s) 880
BisI GCNGC 5 cut(s) 186, 399, 699, 897, 900
BlpI GCTNAGC 1 cut(s) 87
BlsI GCNGC 5 cut(s) 187, 400, 700, 898, 901
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 1 cut(s) 428
BmgBI CACGTC 1 cut(s) 686
BmgT120I GGNCC 1 cut(s) 428
BmiI GGNNCC 2 cut(s) 729, 835
BmrFI CCNGG 1 cut(s) 363
BmrI ACTGGG 2 cut(s) 445, 498
BmsI GCATC 5 cut(s) 226, 248, 397, 649, 815
BmuI ACTGGG 2 cut(s) 445, 498
BpiI GAAGAC 1 cut(s) 791
BplI GAGNNNNNCTC 2 cut(s) 848, 880
Bpu1102I GCTNAGC 1 cut(s) 87
BsaJI CCNNGG 2 cut(s) 393, 625
Bsc4I CCNNNNNNNGG 2 cut(s) 793, 935
Bse1I ACTGG 4 cut(s) 440, 504, 648, 940
Bse3DI GCAATG 1 cut(s) 721
BseBI CCWGG 1 cut(s) 363
BseDI CCNNGG 2 cut(s) 393, 625
BseGI GGATG 2 cut(s) 186, 412
BseLI CCNNNNNNNGG 2 cut(s) 793, 935
BseMI GCAATG 1 cut(s) 721
BseMII CTCAG 3 cut(s) 101, 180, 623
BseNI ACTGG 4 cut(s) 440, 504, 648, 940
BseRI GAGGAG 1 cut(s) 85
BseSI GKGCMC 1 cut(s) 592
BseXI GCAGC 3 cut(s) 197, 385, 710
BsgI GTGCAG 1 cut(s) 399
Bsh1236I CGCG 2 cut(s) 627, 681
BshFI GGCC 1 cut(s) 555
BshNI GGYRCC 1 cut(s) 727
BsiHKAI GWGCWC 3 cut(s) 74, 592, 973
BslI CCNNNNNNNGG 2 cut(s) 793, 935
BsmAI GTCTC 2 cut(s) 169, 856
BsnI GGCC 1 cut(s) 555
Bsp1286I GDGCHC 3 cut(s) 74, 592, 973
Bsp143I GATC 4 cut(s) 783, 811, 833, 906
Bsp1720I GCTNAGC 1 cut(s) 87
BspACI CCGC 7 cut(s) 199, 440, 625, 627, 681, 897, 900
BspANI GGCC 1 cut(s) 555
BspCNI CTCAG 3 cut(s) 100, 179, 622
BspFNI CGCG 2 cut(s) 627, 681
BspLI GGNNCC 2 cut(s) 729, 835
BspMI ACCTGC 1 cut(s) 880
BspPI GGATC 3 cut(s) 778, 828, 841
BspT107I GGYRCC 1 cut(s) 727
BsrDI GCAATG 1 cut(s) 721
BsrI ACTGG 4 cut(s) 440, 504, 648, 940
BssECI CCNNGG 2 cut(s) 393, 625
BssMI GATC 4 cut(s) 783, 811, 833, 906
Bst2UI CCWGG 1 cut(s) 363
Bst6I CTCTTC 1 cut(s) 451
BstAPI GCANNNNNTGC 2 cut(s) 32, 668
BstC8I GCNNGC 4 cut(s) 60, 203, 475, 497
BstDEI CTNAG 4 cut(s) 87, 166, 511, 609
BstDSI CCRYGG 2 cut(s) 393, 625
BstF5I GGATG 2 cut(s) 186, 412
BstFNI CGCG 2 cut(s) 627, 681
BstKTI GATC 4 cut(s) 786, 814, 836, 909
BstMAI GTCTC 2 cut(s) 169, 856
BstMBI GATC 4 cut(s) 783, 811, 833, 906
BstNI CCWGG 1 cut(s) 363
BstSCI CCNGG 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 637
BstSLI GKGCMC 1 cut(s) 592
BstUI CGCG 2 cut(s) 627, 681
BstV1I GCAGC 3 cut(s) 197, 385, 710
BstV2I GAAGAC 1 cut(s) 791
BstX2I RGATCY 1 cut(s) 833
BstYI RGATCY 1 cut(s) 833
BsuRI GGCC 1 cut(s) 555
BtgI CCRYGG 2 cut(s) 393, 625
BtrI CACGTC 1 cut(s) 686
BtsCI GGATG 2 cut(s) 186, 412
BtsI GCAGTG 2 cut(s) 44, 287
BtsIMutI CAGTG 5 cut(s) 44, 287, 617, 655, 933
BveI ACCTGC 1 cut(s) 880
Cac8I GCNNGC 4 cut(s) 60, 203, 475, 497
Cfr13I GGNCC 1 cut(s) 428
Cfr42I CCGCGG 1 cut(s) 628
Csp6I GTAC 2 cut(s) 10, 675
CspCI CAANNNNNGTGG 2 cut(s) 720, 755
CviAII CATG 2 cut(s) 212, 904
CviQI GTAC 2 cut(s) 10, 675
DdeI CTNAG 4 cut(s) 87, 166, 511, 609
DpnI GATC 4 cut(s) 785, 813, 835, 908
DpnII GATC 4 cut(s) 783, 811, 833, 906
DrdI GACNNNNNNGTC 2 cut(s) 690, 848
DseDI GACNNNNNNGTC 2 cut(s) 690, 848
Eam1104I CTCTTC 1 cut(s) 451
EarI CTCTTC 1 cut(s) 451
EciI GGCGGA 1 cut(s) 188
Eco32I GATATC 1 cut(s) 385
Eco47I GGWCC 1 cut(s) 428
Eco57I CTGAAG 2 cut(s) 363, 396
EcoRII CCWGG 1 cut(s) 361
EcoRV GATATC 1 cut(s) 385
EcoT22I ATGCAT 1 cut(s) 241
FaeI CATG 2 cut(s) 215, 907
FaiI YATR 8 cut(s) 108, 213, 318, 326, 358, 861, 905, 964
FatI CATG 2 cut(s) 211, 903
FbaI TGATCA 2 cut(s) 811, 906
Fnu4HI GCNGC 5 cut(s) 186, 399, 699, 897, 900
FokI GGATG 2 cut(s) 193, 419
Fsp4HI GCNGC 5 cut(s) 186, 399, 699, 897, 900
FspBI CTAG 2 cut(s) 402, 485
GluI GCNGC 5 cut(s) 186, 399, 699, 897, 900
HaeIII GGCC 1 cut(s) 555
Hin1II CATG 2 cut(s) 215, 907
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 3 cut(s) 321, 461, 633
HphI GGTGA 2 cut(s) 454, 754
Hpy166II GTNNAC 1 cut(s) 590
Hpy188I TCNGA 7 cut(s) 143, 180, 300, 343, 566, 811, 856
Hpy188III TCNNGA 4 cut(s) 431, 775, 787, 910
Hpy8I GTNNAC 1 cut(s) 590
Hpy99I CGWCG 2 cut(s) 180, 392
HpyAV CCTTC 1 cut(s) 787
HpyCH4IV ACGT 3 cut(s) 175, 685, 708
HpyF3I CTNAG 4 cut(s) 87, 166, 511, 609
HpySE526I ACGT 3 cut(s) 175, 685, 708
Hsp92II CATG 2 cut(s) 215, 907
Ksp22I TGATCA 2 cut(s) 811, 906
KspI CCGCGG 1 cut(s) 628
Kzo9I GATC 4 cut(s) 783, 811, 833, 906
Lsp1109I GCAGC 3 cut(s) 197, 385, 710
LweI GCATC 5 cut(s) 226, 248, 397, 649, 815
MaeI CTAG 2 cut(s) 402, 485
MaeII ACGT 3 cut(s) 175, 685, 708
MaeIII GTNAC 2 cut(s) 650, 742
MalI GATC 4 cut(s) 785, 813, 835, 908
MboI GATC 4 cut(s) 783, 811, 833, 906
MboII GAAGA 5 cut(s) 255, 465, 468, 791, 802
MfeI CAATTG 2 cut(s) 597, 755
MflI RGATCY 1 cut(s) 833
MhlI GDGCHC 3 cut(s) 74, 592, 973
MluCI AATT 3 cut(s) 597, 721, 755
MlyI GAGTC 1 cut(s) 330
MmeI TCCRAC 2 cut(s) 158, 403
Mph1103I ATGCAT 1 cut(s) 241
MroXI GAANNNNTTC 1 cut(s) 460
MseI TTAA 1 cut(s) 924
MslI CAYNNNNRTG 1 cut(s) 210
MspA1I CMGCKG 1 cut(s) 627
MspR9I CCNGG 1 cut(s) 363
MunI CAATTG 2 cut(s) 597, 755
MvaI CCWGG 1 cut(s) 363
MvnI CGCG 2 cut(s) 627, 681
NdeII GATC 4 cut(s) 783, 811, 833, 906
NlaIII CATG 2 cut(s) 215, 907
NlaIV GGNNCC 2 cut(s) 729, 835
NmuCI GTSAC 2 cut(s) 650, 742
NsiI ATGCAT 1 cut(s) 241
PdmI GAANNNNTTC 1 cut(s) 460
PfeI GAWTC 2 cut(s) 461, 633
PkrI GCNGC 5 cut(s) 187, 400, 700, 898, 901
PleI GAGTC 1 cut(s) 329
PpsI GAGTC 1 cut(s) 329
Psp1406I AACGTT 1 cut(s) 708
Psp6I CCWGG 1 cut(s) 361
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 2 cut(s) 729, 835
PspPI GGNCC 1 cut(s) 428
PsuI RGATCY 1 cut(s) 833
RsaI GTAC 2 cut(s) 11, 676
RsaNI GTAC 2 cut(s) 10, 675
RseI CAYNNNNRTG 1 cut(s) 210
SacII CCGCGG 1 cut(s) 628
SaqAI TTAA 1 cut(s) 924
SatI GCNGC 5 cut(s) 186, 399, 699, 897, 900
Sau3AI GATC 4 cut(s) 783, 811, 833, 906
Sau96I GGNCC 1 cut(s) 428
ScaI AGTACT 1 cut(s) 11
SchI GAGTC 1 cut(s) 330
ScrFI CCNGG 1 cut(s) 363
SduI GDGCHC 3 cut(s) 74, 592, 973
SfaNI GCATC 5 cut(s) 226, 248, 397, 649, 815
SfcI CTRYAG 1 cut(s) 637
Sfr303I CCGCGG 1 cut(s) 628
SgrBI CCGCGG 1 cut(s) 628
SinI GGWCC 1 cut(s) 428
SmiMI CAYNNNNRTG 1 cut(s) 210
Sse9I AATT 3 cut(s) 597, 721, 755
SsiI CCGC 7 cut(s) 199, 440, 625, 627, 681, 897, 900
SspMI CTAG 2 cut(s) 402, 485
StyD4I CCNGG 1 cut(s) 361
TaiI ACGT 3 cut(s) 178, 688, 711
TaqI TCGA 3 cut(s) 98, 464, 522
TasI AATT 3 cut(s) 597, 721, 755
TatI WGTACW 1 cut(s) 9
TauI GCSGC 2 cut(s) 899, 902
TfiI GAWTC 2 cut(s) 461, 633
Tru1I TTAA 1 cut(s) 924
Tru9I TTAA 1 cut(s) 924
TscAI CASTG 5 cut(s) 51, 294, 617, 655, 940
TseFI GTSAC 2 cut(s) 650, 742
TseI GCWGC 3 cut(s) 185, 398, 698
Tsp45I GTSAC 2 cut(s) 650, 742
TspDTI ATGAA 1 cut(s) 20
TspRI CASTG 5 cut(s) 51, 294, 617, 655, 940
VneI GTGCAC 1 cut(s) 588
VpaK11BI GGWCC 1 cut(s) 428
XapI RAATTY 1 cut(s) 721
XcmI CCANNNNNNNNNTGG 2 cut(s) 343, 910
XmnI GAANNNNTTC 1 cut(s) 460
XspI CTAG 2 cut(s) 402, 485
ZrmI AGTACT 1 cut(s) 11
Zsp2I ATGCAT 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.