Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003113

Physical Location & Seq

Genomic Coordinates Forward (+)

1119569 .. 1123047

Gene Structure 3479 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1017200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2823 bp

ATGAAGGACCCCCATCTGGGTTTCTGGGGCTGTTTCCCTGTTCTGCTACTTTTGTTCGTTTCACTGTACCCACCATCAGTACACTCCCAGTCCGGCGGCGGCGACGACGGAGCGGCCATGGAAGCACTCAGCAAAAGCATCGGCCCCAACAGTCTCGGGTGGTCCGGCACCGATTACTGCAACTGGGGACAAGTCAGTTGCACCAAGGACGGCAAGGTTTTCAAAATCCAATTGGGTAATCAGAAACTCACCGGCACGCTTCCGCCGGAAATTCAAAAGCTTTCAAATTTGCAGCAACTCGAGGTTCAGAACAACCAACTCACCGGACCTTTTCCGAGCCTCTCCGGGCTGCAACCGCTTCAGGTCCTCCTCGCCCACGACAACAACTTCTCGTCCTTCCCTTCCGATTTCTTCTCCGGACTCACCTCTCTTGACAGCATCAACATCGACCACAACCCCTTCGCAGCGTGGCAGATTCCGGACACTCTTAAAGACGCCACCGCGCTGAGGGAATTCTCCGCAACCGAGACCAACATCACCGGAAGAATCCCCGATATCTTCACCGGTTCCAATTTCCCAGGTTTGACTGAACTGCATTTGGCTTTCAATTACCTTGAAGGCGAATTGCCTGCTAGTTTTTCGAGTTCGAGTATTCAGTCCCTCTGGTTGAACGGCCAACAGGGCACCAATAGGCTTAATGGTACTATTGATGTGTTACAGAACATGACTTCTCTGCGTGAAGTTTGGTTACATGGTAATTTTTTCACCGGTCCTATACCGGACCTGTCGACATTAGGTACCTTGACCACTCTGAGTTTGAGGGATAACAGGCTTACCGGAGTTGTTCCGGCGTCGTTGTTGAATCTTAAATCCCTTACTGTTGTTAATTTGACCAACAATATGCTTCAAGGGCCAATGCCGAAGTTTGGCGATGGGGTTCAGGTGGATATGACATCGCAAAATAGGTTTTGCAGTGATAAGCCGGGTGTTGATTGTGACACTCGTGTCAATATATTGCTTTCGGTCGTCAAAGACATGGGTTATCCTACTGTTTTTGCAGAGAGTTGGAAAGGGAATGATCCTTGTAATAACTGGAAGGGAATTGTGTGTCATGGAGGAAACATCACCGTTGTCATCTTTCGGAGCCTGGGTCTGAGGGGTACGATCTCTACAAATTATTCTCTGATTACCTTCTTGCAAACATTGAGACTTGACAATAATAATCTCACGGGTACTATACCAAAGGAACTTACACAACTGCCCAACCTTCAAGAGATAGATGTTAGTAACAATCAACTTGACGGTCAAGTACCCAAGTTTAATGATACTGTGCAAGTGAAAACAGGTGGGAACCCTCGTATTGTTAAGGATGGTCCCCCTTCGCCAGCGACGCCTCCTAATTCACCGCCTGGCTCACACTCAGGCGGAGGTAAAAAATCTAGGACTGGGGTGGTTGTTGGGGCTGTCATCGGCAGTGTTGGTGGATTGGTTGTAGTTGGGTTTGTTGCTTTTTGTCTACTTAAGAGAAAGCATTCTGGCAGAGTGCAAAGTCCAAACGCGTTGGTTATTCATCCTTGTCATTCTGGAGATCAAAATGCAGTCAAGATCTCGGTTGCTAGCCCTGGGGTTAATTGCGGTGGAAATGAGTCCTATAAGAGTCCGGCAAGTAGTGGACCTAATGACATTCATGTGGTTGAGGCGGAAAGTATGGTCATTTCAATCCAAGTTTTGAGAAATGTGACCAATAATTTCAGTGAAAATAATGTATTAGGAAAAGGTGGGTTTGGAACTGTGTACAAAGGGGAGTTGCATGATGGGACAAAGATTGCAGTGAAGAGGATGGAATCTGGAGTGGTGGCGGAGAAAGGTCTAAATGAGTTCAAGTCTGAGATTGCAGTTCTGACTAAGGTCCGACACCGCCACTTGGTTGGACTTCTTGGCTATTGTTTGGAAGGAAACGAGAGGCTTCTTGTTTATGAATACATGCCTCAAGGGACTCTTAGTAGATACTTGTTCAACTGGATAGAGGAAGGTCTGAAGCCACTTGAATGGACTAGAAGGCTGACCATTGCCTTGGATGTTGCAAGAGGGGTTGAGTATCTCCACGGTTTAGCCAACCAGACTTTCATTCACAGGGATCTTAAACCTTCGAACATTTTACTCGGAGATGATATGCGGGCTAAAGTTTCAGACTTTGGACTGGTTCGTCTTGCTCCAGAAGGGAAAGCCTCAATTGAGACGAGACTAGCCGGAACTTTTGGCTATTTGGCTCCTGAGTATGCAGCAACTGGGCGGATGACACTCAAGGTTGACGTGTATAGCTTTGGAGTGATATTAATGGAGTTGATCACGGGTAGAAAAGCAATTGATGAAAGCCAAGCAGAGGAGAGGTTGCACCTTGTTACATGGTTCCGTAGAATGCTCGTTAACAAGGATGCACTCCGGAAGGTCATTGATCCAACCATTGATCTCAATGAGGAAACTCTTTCTAGTATTAGCACTGTCGCTGAGCTTGCTGGGCATTGCAGTGCAAGGGAGCCCTACCAGAGGCCTGACATGGGTCATGCAGTCAATGTGCTTTCGTCGCTTGTTGAGCAATGGAAACCATCTGAACTCGAAGATTCTGATGATATGCATGGAATTGACCTCGAAATGACCTTACCACAAGCACTGAAGAAGTGGCGGGCTTGTGAGGGCAATAGCAACCTTGATGATTCTTCGTCTTCCTCATCATTGTTTCCCAGCGGGGATAACACCCAAACCAGCATACCTACACGGCCATCTGGATTTGCAGAATCGTTTACATCATCGGATGGGAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

941

Amino Acids

102.12

Weight (kDa)

5.94

Isoelectric Point (pI)

35.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_4	PF12799	399 - 433	2.5e-06	Leucine Rich repeats (2 copies)
Pkinase	PF00069	585 - 857	6.2e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	587 - 816	3.3e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003113)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01820
fragaria_vesca	FvH4_4g33170 FvH4_4g33170 FvH4_4g33171
malus_domestica	MD13G1017200.v1.1 MD16G1015000.v1.1 MD16G1015200.v1.1
prunus_persica	Prupe.1G336300_v2.0.a1 Prupe.1G336300_v2.0.a1
pyrus_communis	pycom13g01480 pycom16g01400
rosa_chinensis	RchiOBHm_Chr4g0442211 RchiOBHm_Chr4g0442221 RchiOBHm_Chr4g0442231
rosa_laevigata	RLG00000006022 RLG00000006023
rosa_multiflora	Rmu_co8222306.1_g000001 Rmu_sc0000124.1_g000060 Rmu_sc0000124.1_g000062 Rmu_sc0010284.1_g000002 Rmu_sc0010284.1_g000003
rosa_roxburghii	Rroxscaffold_5G00383140 Rroxscaffold_5G00383150
rosa_rugosa	Rorug04G0339700 Rorug04G0339800 Rorug04G0339900
rosa_samantha	Rh4AG391300 Rh4AG391400 Rh4BG405000 Rh4BG405100 Rh4CG420100 Rh4CG420200 Rh4DG398100 Rh4DG398200 Rh4DG398300
rosa_wichuraiana	Rw4G033820 Rw4G033830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1004, 2205
Acc65I	GGTACC	1 cut(s)	797
AccB1I	GGYRCC	3 cut(s)	167, 683, 797
AccBSI	CCGCTC	1 cut(s)	113
AccI	GTMKAC	2 cut(s)	788, 1516
AccII	CGCG	2 cut(s)	503, 1559
AccIII	TCCGGA	3 cut(s)	416, 478, 2442
AclWI	GGATC	3 cut(s)	1073, 2145, 2450
AcoI	YGGCCR	3 cut(s)	114, 673, 2777
AcsI	RAATTY	3 cut(s)	270, 286, 512
AcuI	CTGAAG	3 cut(s)	344, 2057, 2693
AcyI	GRCGYC	3 cut(s)	495, 851, 1391
AfaI	GTAC	8 cut(s)	68, 81, 703, 799, 1162, 1234, 1311, 1796
AfiI	CCNNNNNNNGG	8 cut(s)	16, 17, 507, 926, 1924, 2383, 2547, 2558
AflII	CTTAAG	1 cut(s)	1520
AflIII	ACRYGT	2 cut(s)	1557, 2313
AgeI	ACCGGT	2 cut(s)	563, 767
AjiI	CACGTC	1 cut(s)	2314
AjnI	CCWGG	4 cut(s)	577, 1146, 1408, 1621
AjuI	GAANNNNNNNTTGG	2 cut(s)	2108, 2140
AluBI	AGCT	3 cut(s)	280, 2322, 2512
AluI	AGCT	3 cut(s)	280, 2322, 2512
Alw26I	GTCTC	5 cut(s)	158, 521, 1201, 2231, 2236
AlwI	GGATC	3 cut(s)	1073, 2145, 2450
AlwNI	CAGNNNCTG	1 cut(s)	2288
Ama87I	CYCGRG	2 cut(s)	155, 299
Aor13HI	TCCGGA	3 cut(s)	416, 478, 2442
AoxI	GGCC	6 cut(s)	114, 142, 673, 911, 2549, 2777
ApeKI	GCWGC	4 cut(s)	292, 349, 464, 2282
ApoI	RAATTY	3 cut(s)	270, 286, 512
AseI	ATTAAT	1 cut(s)	2336
AsiGI	ACCGGT	2 cut(s)	563, 767
Asp700I	GAANNNNTTC	1 cut(s)	1531
Asp718I	GGTACC	1 cut(s)	797
AspLEI	GCGC	1 cut(s)	505
AsuC2I	CCSGG	2 cut(s)	346, 984
AsuHPI	GGTGA	8 cut(s)	241, 313, 415, 529, 553, 757, 1117, 1395
AsuII	TTCGAA	1 cut(s)	2150
AsuNHI	GCTAGC	1 cut(s)	1616
AvaI	CYCGRG	2 cut(s)	155, 299
AvaII	GGWCC	9 cut(s)	7, 162, 326, 364, 770, 781, 1373, 1673, 1909
BaeGI	GKGCMC	1 cut(s)	686
BanI	GGYRCC	3 cut(s)	167, 683, 797
BanII	GRGCYC	1 cut(s)	2541
BarI	GAAGNNNNNNTAC	2 cut(s)	732, 764
BauI	CACGAG	1 cut(s)	1002
BbsI	GAAGAC	1 cut(s)	2715
BbvCI	CCTCAGC	1 cut(s)	506
BbvI	GCAGC	4 cut(s)	304, 336, 476, 2294
BccI	CCATC	9 cut(s)	21, 82, 926, 1364, 1808, 1834, 2614, 2788, 2807
BceAI	ACGGC	3 cut(s)	226, 688, 2792
BcgI	CGANNNNNNTGC	6 cut(s)	121, 155, 427, 461, 611, 645
BciT130I	CCWGG	4 cut(s)	579, 1148, 1410, 1623
BclI	TGATCA	1 cut(s)	2346
BcnI	CCSGG	2 cut(s)	346, 984
BcoDI	GTCTC	5 cut(s)	158, 521, 1201, 2231, 2236
BfaI	CTAG	6 cut(s)	633, 1440, 1617, 2055, 2246, 2490
BfrI	CTTAAG	1 cut(s)	1520
BglI	GCCNNNNNGGC	1 cut(s)	681
BglII	AGATCT	1 cut(s)	1605
BisI	GCNGC	7 cut(s)	97, 100, 114, 293, 350, 465, 2283
BlpI	GCTNAGC	1 cut(s)	2508
BlsI	GCNGC	7 cut(s)	98, 101, 115, 294, 351, 466, 2284
Bme1390I	CCNGG	6 cut(s)	346, 579, 984, 1148, 1410, 1623
Bme18I	GGWCC	9 cut(s)	7, 162, 326, 364, 770, 781, 1373, 1673, 1909
BmeT110I	CYCGRG	2 cut(s)	155, 299
BmgBI	CACGTC	1 cut(s)	2314
BmrFI	CCNGG	6 cut(s)	346, 579, 984, 1148, 1410, 1623
BmrI	ACTGGG	4 cut(s)	82, 193, 1455, 2298
BmsI	GCATC	3 cut(s)	147, 447, 2425
BmtI	GCTAGC	1 cut(s)	1620
BmuI	ACTGGG	4 cut(s)	82, 193, 1455, 2298
BoxI	GACNNNNGTC	2 cut(s)	1907, 2559
BpiI	GAAGAC	1 cut(s)	2715
BpmI	CTGGAG	3 cut(s)	1605, 1869, 2199
Bpu10I	CCTNAGC	1 cut(s)	506
Bpu1102I	GCTNAGC	1 cut(s)	2508
Bpu14I	TTCGAA	1 cut(s)	2150
BpuEI	CTTGAG	2 cut(s)	1974, 2288
BpuMI	CCSGG	2 cut(s)	346, 984
BsaHI	GRCGYC	3 cut(s)	495, 851, 1391
BsaI	GGTCTC	1 cut(s)	521
BsaJI	CCNNGG	8 cut(s)	117, 204, 577, 1147, 1621, 1622, 2073, 2104
BsaWI	WCCGGW	9 cut(s)	323, 416, 478, 539, 563, 767, 778, 836, 2442
BsaXI	ACNNNNNCTCC	2 cut(s)	1135, 1165
Bsc4I	CCNNNNNNNGG	8 cut(s)	16, 17, 507, 926, 1924, 2383, 2547, 2558
Bse118I	RCCGGY	3 cut(s)	251, 563, 767
Bse1I	ACTGG	7 cut(s)	88, 188, 1097, 1450, 2024, 2205, 2293
Bse3DI	GCAATG	3 cut(s)	2067, 2521, 2603
BseAI	TCCGGA	3 cut(s)	416, 478, 2442
BseBI	CCWGG	4 cut(s)	579, 1148, 1410, 1623
BseDI	CCNNGG	8 cut(s)	117, 204, 577, 1147, 1621, 1622, 2073, 2104
BseGI	GGATG	7 cut(s)	1375, 1570, 1845, 2083, 2301, 2440, 2818
BseLI	CCNNNNNNNGG	8 cut(s)	16, 17, 507, 926, 1924, 2383, 2547, 2558
BseMI	GCAATG	3 cut(s)	2067, 2521, 2603
BseMII	CTCAG	8 cut(s)	142, 497, 803, 1145, 1434, 1878, 2265, 2499
BseNI	ACTGG	7 cut(s)	88, 188, 1097, 1450, 2024, 2205, 2293
BseRI	GAGGAG	2 cut(s)	359, 2399
BseSI	GKGCMC	1 cut(s)	686
BseXI	GCAGC	4 cut(s)	304, 336, 476, 2294
BseYI	CCCAGC	2 cut(s)	2516, 2741
Bsh1236I	CGCG	2 cut(s)	503, 1559
Bsh1285I	CGRYCG	1 cut(s)	1026
BshFI	GGCC	6 cut(s)	116, 144, 675, 913, 2551, 2779
BshNI	GGYRCC	3 cut(s)	167, 683, 797
BshTI	ACCGGT	2 cut(s)	563, 767
BsiEI	CGRYCG	1 cut(s)	1026
BsiHKCI	CYCGRG	2 cut(s)	155, 299
BslFI	GGGAC	5 cut(s)	201, 643, 1359, 1831, 2008
BslI	CCNNNNNNNGG	8 cut(s)	16, 17, 507, 926, 1924, 2383, 2547, 2558
BsmAI	GTCTC	5 cut(s)	158, 521, 1201, 2231, 2236
BsmBI	CGTCTC	1 cut(s)	2231
BsmFI	GGGAC	5 cut(s)	201, 643, 1359, 1831, 2008
BsmI	GAATGC	2 cut(s)	1531, 2424
BsnI	GGCC	6 cut(s)	116, 144, 675, 913, 2551, 2779
Bso31I	GGTCTC	1 cut(s)	521
BsoBI	CYCGRG	2 cut(s)	155, 299
Bsp119I	TTCGAA	1 cut(s)	2150
Bsp1286I	GDGCHC	2 cut(s)	686, 2541
Bsp13I	TCCGGA	3 cut(s)	416, 478, 2442
Bsp1407I	TGTACA	1 cut(s)	1794
Bsp143I	GATC	8 cut(s)	1078, 1164, 1588, 1605, 2137, 2346, 2455, 2467
Bsp1720I	GCTNAGC	1 cut(s)	2508
Bsp19I	CCATGG	1 cut(s)	117
BspANI	GGCC	6 cut(s)	116, 144, 675, 913, 2551, 2779
BspCNI	CTCAG	8 cut(s)	141, 498, 804, 1146, 1433, 1879, 2266, 2500
BspEI	TCCGGA	3 cut(s)	416, 478, 2442
BspFNI	CGCG	2 cut(s)	503, 1559
BspOI	GCTAGC	1 cut(s)	1620
BspPI	GGATC	3 cut(s)	1073, 2145, 2450
BspT104I	TTCGAA	1 cut(s)	2150
BspT107I	GGYRCC	3 cut(s)	167, 683, 797
BspTI	CTTAAG	1 cut(s)	1520
BspTNI	GGTCTC	1 cut(s)	521
BsrBI	CCGCTC	1 cut(s)	113
BsrDI	GCAATG	3 cut(s)	2067, 2521, 2603
BsrFI	RCCGGY	3 cut(s)	251, 563, 767
BsrGI	TGTACA	1 cut(s)	1794
BsrI	ACTGG	7 cut(s)	88, 188, 1097, 1450, 2024, 2205, 2293
BssAI	RCCGGY	3 cut(s)	251, 563, 767
BssECI	CCNNGG	8 cut(s)	117, 204, 577, 1147, 1621, 1622, 2073, 2104
BssMI	GATC	8 cut(s)	1078, 1164, 1588, 1605, 2137, 2346, 2455, 2467
BssNI	GRCGYC	3 cut(s)	495, 851, 1391
BssSI	CACGAG	1 cut(s)	1002
BssT1I	CCWWGG	3 cut(s)	117, 204, 2073
Bst2BI	CACGAG	1 cut(s)	1002
Bst2UI	CCWGG	4 cut(s)	579, 1148, 1410, 1623
Bst6I	CTCTTC	1 cut(s)	1829
BstACI	GRCGYC	3 cut(s)	495, 851, 1391
BstAFI	CTTAAG	1 cut(s)	1520
BstAUI	TGTACA	1 cut(s)	1794
BstBI	TTCGAA	1 cut(s)	2150
BstC8I	GCNNGC	7 cut(s)	257, 630, 1386, 1618, 2178, 2514, 2685
BstDSI	CCRYGG	2 cut(s)	117, 2104
BstENI	CCTNNNNNAGG	1 cut(s)	2545
BstF5I	GGATG	7 cut(s)	1375, 1570, 1845, 2083, 2301, 2440, 2818
BstFNI	CGCG	2 cut(s)	503, 1559
BstHHI	GCGC	1 cut(s)	505
BstKTI	GATC	8 cut(s)	1081, 1167, 1591, 1608, 2140, 2349, 2458, 2470
BstMAI	GTCTC	5 cut(s)	158, 521, 1201, 2231, 2236
BstMBI	GATC	8 cut(s)	1078, 1164, 1588, 1605, 2137, 2346, 2455, 2467
BstMCI	CGRYCG	1 cut(s)	1026
BstMWI	GCNNNNNNNGC	9 cut(s)	122, 355, 681, 910, 1390, 2513, 2518, 2584, 2593
BstNI	CCWGG	4 cut(s)	579, 1148, 1410, 1623
BstNSI	RCATGY	1 cut(s)	1987
BstPAI	GACNNNNGTC	2 cut(s)	1907, 2559
BstSCI	CCNGG	6 cut(s)	344, 577, 982, 1146, 1408, 1621
BstSLI	GKGCMC	1 cut(s)	686
BstUI	CGCG	2 cut(s)	503, 1559
BstV1I	GCAGC	4 cut(s)	304, 336, 476, 2294
BstV2I	GAAGAC	1 cut(s)	2715
BstX2I	RGATCY	2 cut(s)	1605, 2137
BstXI	CCANNNNNNTGG	3 cut(s)	1928, 2049, 2074
BstYI	RGATCY	2 cut(s)	1605, 2137
BsuRI	GGCC	6 cut(s)	116, 144, 675, 913, 2551, 2779
BtgI	CCRYGG	2 cut(s)	117, 2104
BtgZI	GCGATG	2 cut(s)	939, 945
BtrI	CACGTC	1 cut(s)	2314
BtsCI	GGATG	7 cut(s)	1375, 1570, 1845, 2083, 2301, 2440, 2818
BtsI	GCAGTG	4 cut(s)	979, 1480, 1836, 2533
BtsIMutI	CAGTG	8 cut(s)	62, 979, 1480, 1759, 1836, 2499, 2533, 2669
Cac8I	GCNNGC	7 cut(s)	257, 630, 1386, 1618, 2178, 2514, 2685
CaiI	CAGNNNCTG	1 cut(s)	2288
CfoI	GCGC	1 cut(s)	505
Cfr10I	RCCGGY	3 cut(s)	251, 563, 767
CseI	GACGC	3 cut(s)	503, 840, 1399
Csp6I	GTAC	8 cut(s)	67, 80, 702, 798, 1161, 1233, 1310, 1795
CspAI	ACCGGT	2 cut(s)	563, 767
CviQI	GTAC	8 cut(s)	67, 80, 702, 798, 1161, 1233, 1310, 1795
DpnI	GATC	8 cut(s)	1080, 1166, 1590, 1607, 2139, 2348, 2457, 2469
DpnII	GATC	8 cut(s)	1078, 1164, 1588, 1605, 2137, 2346, 2455, 2467
DrdI	GACNNNNNNGTC	2 cut(s)	1004, 2205
DseDI	GACNNNNNNGTC	2 cut(s)	1004, 2205
EaeI	YGGCCR	3 cut(s)	114, 673, 2777
Eam1104I	CTCTTC	1 cut(s)	1829
EarI	CTCTTC	1 cut(s)	1829
EciI	GGCGGA	5 cut(s)	252, 1440, 1715, 1874, 2308
Eco130I	CCWWGG	3 cut(s)	117, 204, 2073
Eco147I	AGGCCT	1 cut(s)	2551
Eco24I	GRGCYC	1 cut(s)	2541
Eco31I	GGTCTC	1 cut(s)	521
Eco32I	GATATC	1 cut(s)	556
Eco47I	GGWCC	9 cut(s)	7, 162, 326, 364, 770, 781, 1373, 1673, 1909
Eco57I	CTGAAG	3 cut(s)	344, 2057, 2693
Eco88I	CYCGRG	2 cut(s)	155, 299
EcoNI	CCTNNNNNAGG	1 cut(s)	2545
EcoO109I	RGGNCCY	2 cut(s)	7, 364
EcoRI	GAATTC	1 cut(s)	512
EcoRII	CCWGG	4 cut(s)	577, 1146, 1408, 1621
EcoRV	GATATC	1 cut(s)	556
EcoT14I	CCWWGG	3 cut(s)	117, 204, 2073
EcoT22I	ATGCAT	1 cut(s)	2637
EcoT38I	GRGCYC	1 cut(s)	2541
ErhI	CCWWGG	3 cut(s)	117, 204, 2073
Esp3I	CGTCTC	1 cut(s)	2231
FalI	AAGNNNNNCTT	2 cut(s)	1983, 2015
FaqI	GGGAC	5 cut(s)	201, 643, 1359, 1831, 2008
FauI	CCCGC	3 cut(s)	2169, 2676, 2738
FbaI	TGATCA	1 cut(s)	2346
FblI	GTMKAC	2 cut(s)	788, 1516
Fnu4HI	GCNGC	7 cut(s)	97, 100, 114, 293, 350, 465, 2283
FokI	GGATG	6 cut(s)	1382, 1557, 1852, 2090, 2308, 2447
FriOI	GRGCYC	1 cut(s)	2541
Fsp4HI	GCNGC	7 cut(s)	97, 100, 114, 293, 350, 465, 2283
FspBI	CTAG	6 cut(s)	633, 1440, 1617, 2055, 2246, 2490
GlaI	GCGC	1 cut(s)	504
GluI	GCNGC	7 cut(s)	97, 100, 114, 293, 350, 465, 2283
GsaI	CCCAGC	2 cut(s)	2520, 2745
GsuI	CTGGAG	3 cut(s)	1605, 1869, 2199
HaeIII	GGCC	6 cut(s)	116, 144, 675, 913, 2551, 2779
HgaI	GACGC	3 cut(s)	503, 840, 1399
HhaI	GCGC	1 cut(s)	505
Hin1I	GRCGYC	3 cut(s)	495, 851, 1391
Hin6I	GCGC	1 cut(s)	503
HinP1I	GCGC	1 cut(s)	503
HincII	GTYRAC	3 cut(s)	789, 2311, 2428
HindII	GTYRAC	3 cut(s)	789, 2311, 2428
HindIII	AAGCTT	1 cut(s)	278
HpaI	GTTAAC	1 cut(s)	2428
HphI	GGTGA	8 cut(s)	241, 313, 415, 529, 553, 757, 1117, 1395
Hpy166II	GTNNAC	8 cut(s)	82, 789, 1517, 1673, 1795, 2311, 2428, 2802
Hpy8I	GTNNAC	8 cut(s)	82, 789, 1517, 1673, 1795, 2311, 2428, 2802
Hpy99I	CGWCG	5 cut(s)	107, 110, 856, 1393, 2587
HpyCH4IV	ACGT	1 cut(s)	2313
HpyF10VI	GCNNNNNNNGC	9 cut(s)	122, 355, 681, 910, 1390, 2513, 2518, 2584, 2593
HpySE526I	ACGT	1 cut(s)	2313
Hsp92I	GRCGYC	3 cut(s)	495, 851, 1391
HspAI	GCGC	1 cut(s)	503
Kpn2I	TCCGGA	3 cut(s)	416, 478, 2442
KpnI	GGTACC	1 cut(s)	801
Ksp22I	TGATCA	1 cut(s)	2346
KspAI	GTTAAC	1 cut(s)	2428
Kzo9I	GATC	8 cut(s)	1078, 1164, 1588, 1605, 2137, 2346, 2455, 2467
LmnI	GCTCC	5 cut(s)	110, 1143, 2218, 2275, 2536
Lsp1109I	GCAGC	4 cut(s)	304, 336, 476, 2294
LweI	GCATC	3 cut(s)	147, 447, 2425
MaeI	CTAG	6 cut(s)	633, 1440, 1617, 2055, 2246, 2490
MaeII	ACGT	1 cut(s)	2313
MaeIII	GTNAC	6 cut(s)	714, 747, 995, 1286, 1738, 2401
MalI	GATC	8 cut(s)	1080, 1166, 1590, 1607, 2139, 2348, 2457, 2469
MbiI	CCGCTC	1 cut(s)	113
MboI	GATC	8 cut(s)	1078, 1164, 1588, 1605, 2137, 2346, 2455, 2467
MboII	GAAGA	8 cut(s)	403, 550, 555, 1846, 2630, 2686, 2709, 2715
MfeI	CAATTG	3 cut(s)	230, 2232, 2364
MflI	RGATCY	2 cut(s)	1605, 2137
MhlI	GDGCHC	2 cut(s)	686, 2541
MluI	ACGCGT	1 cut(s)	1557
MlyI	GAGTC	4 cut(s)	414, 1655, 1666, 1990
MmeI	TCCRAC	4 cut(s)	1046, 1909, 1936, 2483
Mph1103I	ATGCAT	1 cut(s)	2637
MroI	TCCGGA	3 cut(s)	416, 478, 2442
MroXI	GAANNNNTTC	1 cut(s)	1531
MslI	CAYNNNNRTG	3 cut(s)	1688, 2047, 2526
MspA1I	CMGCKG	1 cut(s)	2745
MspCI	CTTAAG	1 cut(s)	1520
MspR9I	CCNGG	6 cut(s)	346, 579, 984, 1148, 1410, 1623
MunI	CAATTG	3 cut(s)	230, 2232, 2364
Mva1269I	GAATGC	2 cut(s)	1531, 2424
MvaI	CCWGG	4 cut(s)	579, 1148, 1410, 1623
MvnI	CGCG	2 cut(s)	503, 1559
MwoI	GCNNNNNNNGC	9 cut(s)	122, 355, 681, 910, 1390, 2513, 2518, 2584, 2593
NciI	CCSGG	2 cut(s)	346, 984
NcoI	CCATGG	1 cut(s)	117
NdeII	GATC	8 cut(s)	1078, 1164, 1588, 1605, 2137, 2346, 2455, 2467
NheI	GCTAGC	1 cut(s)	1616
NmuCI	GTSAC	2 cut(s)	995, 1738
NsiI	ATGCAT	1 cut(s)	2637
NspI	RCATGY	1 cut(s)	1987
NspV	TTCGAA	1 cut(s)	2150
PaeR7I	CTCGAG	1 cut(s)	299
PasI	CCCWGGG	1 cut(s)	1622
PceI	AGGCCT	1 cut(s)	2551
PctI	GAATGC	2 cut(s)	1531, 2424
PdmI	GAANNNNTTC	1 cut(s)	1531
PfeI	GAWTC	7 cut(s)	475, 546, 862, 1844, 2621, 2714, 2795
PinAI	ACCGGT	2 cut(s)	563, 767
PkrI	GCNGC	7 cut(s)	98, 101, 115, 294, 351, 466, 2284
PleI	GAGTC	4 cut(s)	414, 1654, 1665, 1990
PpsI	GAGTC	4 cut(s)	414, 1654, 1665, 1990
PpuMI	RGGWCCY	2 cut(s)	7, 364
PshAI	GACNNNNGTC	2 cut(s)	1907, 2559
PshBI	ATTAAT	1 cut(s)	2336
Psp5II	RGGWCCY	2 cut(s)	7, 364
Psp6I	CCWGG	4 cut(s)	577, 1146, 1408, 1621
PspFI	CCCAGC	2 cut(s)	2516, 2741
PspGI	CCWGG	4 cut(s)	577, 1146, 1408, 1621
PspPPI	RGGWCCY	2 cut(s)	7, 364
PspXI	VCTCGAGB	1 cut(s)	299
PstNI	CAGNNNCTG	1 cut(s)	2288
PsuI	RGATCY	2 cut(s)	1605, 2137
RsaI	GTAC	8 cut(s)	68, 81, 703, 799, 1162, 1234, 1311, 1796
RsaNI	GTAC	8 cut(s)	67, 80, 702, 798, 1161, 1233, 1310, 1795
RseI	CAYNNNNRTG	3 cut(s)	1688, 2047, 2526
SalI	GTCGAC	1 cut(s)	787
SatI	GCNGC	7 cut(s)	97, 100, 114, 293, 350, 465, 2283
Sau3AI	GATC	8 cut(s)	1078, 1164, 1588, 1605, 2137, 2346, 2455, 2467
SchI	GAGTC	4 cut(s)	414, 1655, 1666, 1990
ScrFI	CCNGG	6 cut(s)	346, 579, 984, 1148, 1410, 1623
SduI	GDGCHC	2 cut(s)	686, 2541
SfaNI	GCATC	3 cut(s)	147, 447, 2425
Sfr274I	CTCGAG	1 cut(s)	299
SfuI	TTCGAA	1 cut(s)	2150
SinI	GGWCC	9 cut(s)	7, 162, 326, 364, 770, 781, 1373, 1673, 1909
SlaI	CTCGAG	1 cut(s)	299
SmiMI	CAYNNNNRTG	3 cut(s)	1688, 2047, 2526
SmlI	CTYRAG	4 cut(s)	299, 1520, 1989, 2303
SmoI	CTYRAG	4 cut(s)	299, 1520, 1989, 2303
SseBI	AGGCCT	1 cut(s)	2551
SspMI	CTAG	6 cut(s)	633, 1440, 1617, 2055, 2246, 2490
StuI	AGGCCT	1 cut(s)	2551
StyD4I	CCNGG	6 cut(s)	344, 577, 982, 1146, 1408, 1621
StyI	CCWWGG	3 cut(s)	117, 204, 2073
TaiI	ACGT	1 cut(s)	2316
TaqI	TCGA	8 cut(s)	300, 447, 641, 647, 788, 2150, 2616, 2649
TaqII	GACCGA	1 cut(s)	1012
TatI	WGTACW	2 cut(s)	79, 1794
TauI	GCSGC	3 cut(s)	99, 102, 116
TfiI	GAWTC	7 cut(s)	475, 546, 862, 1844, 2621, 2714, 2795
TscAI	CASTG	8 cut(s)	69, 979, 1480, 1759, 1836, 2506, 2533, 2676
TseFI	GTSAC	2 cut(s)	995, 1738
TseI	GCWGC	4 cut(s)	292, 349, 464, 2282
Tsp45I	GTSAC	2 cut(s)	995, 1738
TspDTI	ATGAA	6 cut(s)	17, 1559, 1676, 1992, 2116, 2385
TspGWI	ACGGA	2 cut(s)	123, 2402
TspRI	CASTG	8 cut(s)	69, 979, 1480, 1759, 1836, 2506, 2533, 2676
Vha464I	CTTAAG	1 cut(s)	1520
VpaK11BI	GGWCC	9 cut(s)	7, 162, 326, 364, 770, 781, 1373, 1673, 1909
VspI	ATTAAT	1 cut(s)	2336
XagI	CCTNNNNNAGG	1 cut(s)	2545
XapI	RAATTY	3 cut(s)	270, 286, 512
XceI	RCATGY	1 cut(s)	1987
XhoI	CTCGAG	1 cut(s)	299
XmiI	GTMKAC	2 cut(s)	788, 1516
XmnI	GAANNNNTTC	1 cut(s)	1531
XspI	CTAG	6 cut(s)	633, 1440, 1617, 2055, 2246, 2490
Zsp2I	ATGCAT	1 cut(s)	2637

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.