Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0003113

Physical Location & Seq

Genomic Coordinates Forward (+)

60966893 .. 60970456

Gene Structure 3564 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G033830.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2643 bp

ATGGGTTTCTGGGGTTGTGTCTTCGTTTTGCTGTCACTGTCCGCACCTGTGCATTCCCAGTCCCAAAAGCTCAGCGAGGACCAGCCAGCCATGGAAGCTATTCTCGAAAACATCAAACCAAAACCACCCACGAATTGGTCGTCTGGCTCCGATTGCTGTACCTGGGGGGACGTAACGTGCGAAAATGGCAAGGTGGTCACCATCATGATCAAAGATCAACATCTCTCCGGCTCTCTCCATCCAGACATCAATAAGCTGACTTCTTTGCTGCGTCTGCACATTATGCGCAACAATCTCACCGGACCCTTCCCAACCCTACAAGGCTTGGCAGAGCTTGAGGAGATTTATGCCAACCAGAACAACTTCACTTATTTTCCAGTAGATGATTTCTTCTCCGGCCTCGATTCCCTTCAAGAATTCCACGTCGACTACAACCCCTTCCAGGTGCGGCAGATTCCGGATAGCACTAAGTTTGCCTCCAAGCTGACCAGTTTCACAGCTAGCGATGCAACCATTTCCGGAAAGACCCCGGAATTTTTTGACAGCAACAATATGCCTAGTTTGGTTACCTTGGAGTTGGCTTATAATAATCTCGAAGGTGAATTGCCTGCCAGTTTTTCCGGTTCCGGGATTCTCAATGGGACTATCTCTGTGTTACAGAACATGACCAGCTTGACTCGAGTTTGGCTACACGGTAATCAATTCACGGGTCCTTTGCCAGAATTTTCAGAGCTTACCGACTTATTGGATTTGAATTTGGGGCATAACAAGCTCACAGGTATTGTTCCTGCGTCTTTGCAAAACCTCAGTTTGCTTCAGAATGTCAGTTTGTCCAGCAATTTGCTTCAAGGACCTATGCCCAATTTCACCGGTGGTGCTATTAAGCAAGTGGACGTGACATCTAATAACTTTTGCACCAATGATGCTGGTACTAAATGCGATCCCCGCGTGACTATATTGCTCTCCATTGCCAAAGACATGGGTTACCCTACTGCTTTTGCAGAGAATTGGATAGGGAATGATCCTTGTGGTGGTAGTACTCATTGGACTGGTCTAACATGTTCAGGAAAAGATGACATTACAGTTCTCAATTTTCCCAACATGGGTCTGACGGGTACGATTTCTTCCAATTTTTCATTGCTTACATCCTTGAATAGATTGATACTTAGAGATAATAGTCTAACCGGTATTATACCCCAAGAGCTCACAAATTTGGACAACCTTGAAGAAATAGATCTCTCAAACAATAAACTTTGTGGTAAAGTACCTAGCTTTCGAGAAAATGTGGTGGTTAACAAGAAATCCAAGACTGGTGTGATTGTTGGGGTTGTTATGGGATGTATTGTTGGAGCACTTATAGTTGGTTTTCTTGCTTTTTATTTGCTTAAGAGAAAACATAGTCGTTATGGTAAAGTGCAAAGTCCAAATTCATTGGTTATACATCCTCGTCACTCTGGTGATCAAAATGCTGTCAAAATTACTGTTACTAACAGGGTTAATGGCATTGGAAGTGAAAATCACAGTAACGGACCTAATGATATTCAAGTTGAGGCTGGAAATATGATAATTTCAATCCAGGTTTTGAGGGGTGTGACCAATAACTTCAATGAAGATAAGATATTGGGAAAAGGTGGGTTTGGAACTGTGTATGAAGGAGAATTGCATGATGGAACGAAAATTGCAGTGAAGAGAATGGAATCCGGAGTGATTGCAGTTCTTACTAAGGTCCGACATCGCCACTTGGTGGGACTTCTTGGATATTGTTTGGATGGGAATGAGAGGCTTCTTGTCTATGAGCACATGCCGCAGGGGACGCTTAGTAGACATTTAAGACTAACCATTGCCTTGGATGTTGCGAGGGGTGTTCAATACCTGCATGGTTTGGCCAACGAAATCTTCATTCATAGAGACCTTAAACCTTCAAATATTTTACTTGGAGATCATGATATGCGGGCGAAAGTGGCAGATTTTGGATTGGTTTGTCTTGCTCCAGCAGGGAAAGCCTCTATTGAGACAAGGCTAGCAGGAACTTTTGGCTATTTGGCTCCAGAATATGCAGATGTTTACAGCTTCGGAGTAATATTAATGGAGTTGGTCACGGGAAGAAAAGCAATTGACGAAAGCCAGCCTGAGGAGAGTTGTCACCTAGTGACATGGTTTCGTAGAAAGATCATTAAAACAGATGCACTCCCAGAGATGGTAGGTCAAGCAGTCAATGTACTTTCATCGCTTGTTGAGCAATGGAAGCCATCTAAGCAAGAAGATTTGGATGAGATGGATGCAATCAACTTCGATGTGCCGTTAAATGAAGCAGTAAAGATGTGGCAGGCTGCGGAGGGCAAAAGCAACATTGATGAATCCTCATCTTCATCGATCTTTCCTAGCGGGCATACCACCCAGACCAGCCTTCCTAATCGGCCTTGTGGATTTGCAGAGTCGATCGTTTACATCATCAGATGGGCGGTAAAGATATTGAGAGTTACCTTGGTGGCTTGTAGTGTTATTTATACATCTGCTGCTTCATGTTTGTTCCTCTTGTGCTTTTCAGATTTTAGTTGTTGCCAACTTTTAAGTCAGAGTAGTTTCAAATGTTCTGGTACATTAGAGCTTGTGGAGATGACTACTAGACAGAATTATCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

880

Amino Acids

96.36

Weight (kDa)

5.74

Isoelectric Point (pI)

33.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	535 - 714	6e-28	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	538 - 718	1.3e-24	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003113)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01820
fragaria_vesca	FvH4_4g33170 FvH4_4g33170 FvH4_4g33171
malus_domestica	MD13G1017200.v1.1 MD16G1015000.v1.1 MD16G1015200.v1.1
prunus_persica	Prupe.1G336300_v2.0.a1 Prupe.1G336300_v2.0.a1
pyrus_communis	pycom13g01480 pycom16g01400
rosa_chinensis	RchiOBHm_Chr4g0442211 RchiOBHm_Chr4g0442221 RchiOBHm_Chr4g0442231
rosa_laevigata	RLG00000006022 RLG00000006023
rosa_multiflora	Rmu_co8222306.1_g000001 Rmu_sc0000124.1_g000060 Rmu_sc0000124.1_g000062 Rmu_sc0010284.1_g000002 Rmu_sc0010284.1_g000003
rosa_roxburghii	Rroxscaffold_5G00383140 Rroxscaffold_5G00383150
rosa_rugosa	Rorug04G0339700 Rorug04G0339800 Rorug04G0339900
rosa_samantha	Rh4AG391300 Rh4AG391400 Rh4BG405000 Rh4BG405100 Rh4CG420100 Rh4CG420200 Rh4DG398100 Rh4DG398200 Rh4DG398300
rosa_wichuraiana	Rw4G033820 Rw4G033830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	585
Acc16I	TGCGCA	1 cut(s)	287
Acc36I	ACCTGC	1 cut(s)	1881
AccB7I	CCANNNNNTGG	2 cut(s)	135, 1744
AccI	GTMKAC	2 cut(s)	426, 1822
AccII	CGCG	1 cut(s)	948
AccIII	TCCGGA	3 cut(s)	457, 518, 1700
AciI	CCGC	8 cut(s)	42, 448, 946, 1805, 1951, 2333, 2385, 2462
AclWI	GGATC	2 cut(s)	935, 1016
AcoI	YGGCCR	1 cut(s)	1884
AcsI	RAATTY	6 cut(s)	416, 533, 722, 754, 1210, 1426
AcuI	CTGAAG	1 cut(s)	800
AdeI	CACNNNGTG	2 cut(s)	1744, 2149
AfaI	GTAC	7 cut(s)	160, 931, 1039, 1117, 1266, 2220, 2599
AfiI	CCNNNNNNNGG	8 cut(s)	135, 442, 627, 1031, 1103, 1744, 2131, 2197
AflII	CTTAAG	1 cut(s)	1385
AflIII	ACRYGT	1 cut(s)	1058
AgeI	ACCGGT	2 cut(s)	869, 1184
AjiI	CACGTC	2 cut(s)	424, 895
AjnI	CCWGG	3 cut(s)	161, 441, 1575
AjuI	GAANNNNNNNTTGG	2 cut(s)	1880, 1912
AleI	CACNNNNGTG	1 cut(s)	1455
Alw21I	GWGCWC	3 cut(s)	1206, 1354, 1800
Alw26I	GTCTC	2 cut(s)	1902, 2006
AlwI	GGATC	2 cut(s)	935, 1016
Ama87I	CYCGRG	1 cut(s)	678
Aor13HI	TCCGGA	3 cut(s)	457, 518, 1700
AoxI	GGCC	3 cut(s)	397, 1884, 2417
ApeKI	GCWGC	3 cut(s)	268, 2330, 2516
ApoI	RAATTY	6 cut(s)	416, 533, 722, 754, 1210, 1426
ArsI	GACNNNNNNTTYG	2 cut(s)	125, 157
AseI	ATTAAT	1 cut(s)	2084
AsiGI	ACCGGT	2 cut(s)	869, 1184
Asp700I	GAANNNNTTC	2 cut(s)	99, 362
AspLEI	GCGC	1 cut(s)	288
AspS9I	GGNCC	6 cut(s)	79, 302, 710, 851, 1529, 1726
AsuC2I	CCSGG	2 cut(s)	530, 628
AsuHPI	GGTGA	6 cut(s)	190, 289, 611, 859, 1469, 2135
AsuNHI	GCTAGC	2 cut(s)	500, 2020
AvaI	CYCGRG	1 cut(s)	678
AvaII	GGWCC	6 cut(s)	79, 302, 710, 851, 1529, 1726
AxyI	CCTNAGG	1 cut(s)	2130
BalI	TGGCCA	1 cut(s)	1886
BanII	GRGCYC	1 cut(s)	1206
BarI	GAAGNNNNNNTAC	2 cut(s)	1108, 1140
BbsI	GAAGAC	1 cut(s)	13
Bbv12I	GWGCWC	3 cut(s)	1206, 1354, 1800
BbvI	GCAGC	3 cut(s)	255, 2317, 2503
BccI	CCATC	8 cut(s)	209, 246, 1661, 1763, 2191, 2257, 2269, 2451
BceAI	ACGGC	1 cut(s)	2284
BciT130I	CCWGG	3 cut(s)	163, 443, 1577
BclI	TGATCA	2 cut(s)	207, 1459
BcnI	CCSGG	2 cut(s)	530, 628
BcoDI	GTCTC	2 cut(s)	1902, 2006
BfaI	CTAG	7 cut(s)	501, 558, 1269, 2021, 2147, 2382, 2625
BfrI	CTTAAG	1 cut(s)	1385
BfuAI	ACCTGC	1 cut(s)	1881
BglII	AGATCT	1 cut(s)	1234
BisI	GCNGC	5 cut(s)	269, 449, 1805, 2331, 2517
BlpI	GCTNAGC	1 cut(s)	71
BlsI	GCNGC	5 cut(s)	270, 450, 1806, 2332, 2518
BmcAI	AGTACT	1 cut(s)	1039
Bme1390I	CCNGG	5 cut(s)	163, 443, 530, 628, 1577
Bme18I	GGWCC	6 cut(s)	79, 302, 710, 851, 1529, 1726
BmeT110I	CYCGRG	1 cut(s)	678
BmgBI	CACGTC	2 cut(s)	424, 895
BmgT120I	GGNCC	6 cut(s)	79, 302, 710, 851, 1529, 1726
BmiI	GGNNCC	5 cut(s)	148, 304, 625, 711, 2046
BmrFI	CCNGG	5 cut(s)	163, 443, 530, 628, 1577
BmrI	ACTGGG	1 cut(s)	52
BmsI	GCATC	4 cut(s)	496, 913, 2173, 2269
BmtI	GCTAGC	2 cut(s)	504, 2024
BmuI	ACTGGG	1 cut(s)	52
BpiI	GAAGAC	1 cut(s)	13
BpmI	CTGGAG	2 cut(s)	1974, 2031
Bpu1102I	GCTNAGC	1 cut(s)	71
BpuEI	CTTGAG	1 cut(s)	356
BpuMI	CCSGG	2 cut(s)	530, 628
Bsa29I	ATCGAT	1 cut(s)	2372
BsaBI	GATNNNNATC	1 cut(s)	219
BsaI	GGTCTC	1 cut(s)	1902
BsaJI	CCNNGG	6 cut(s)	90, 162, 528, 570, 1845, 2484
BsaWI	WCCGGW	7 cut(s)	299, 457, 518, 620, 869, 1184, 1700
Bsc4I	CCNNNNNNNGG	8 cut(s)	135, 442, 627, 1031, 1103, 1744, 2131, 2197
Bse118I	RCCGGY	2 cut(s)	869, 1184
Bse1I	ACTGG	6 cut(s)	58, 377, 489, 612, 1054, 1315
Bse21I	CCTNAGG	1 cut(s)	2130
Bse3DI	GCAATG	4 cut(s)	966, 1136, 1839, 2246
Bse8I	GATNNNNATC	1 cut(s)	219
BseAI	TCCGGA	3 cut(s)	457, 518, 1700
BseBI	CCWGG	3 cut(s)	163, 443, 1577
BseCI	ATCGAT	1 cut(s)	2372
BseDI	CCNNGG	6 cut(s)	90, 162, 528, 570, 1845, 2484
BseGI	GGATG	8 cut(s)	238, 1145, 1343, 1441, 1774, 1855, 2275, 2284
BseJI	GATNNNNATC	1 cut(s)	219
BseLI	CCNNNNNNNGG	8 cut(s)	135, 442, 627, 1031, 1103, 1744, 2131, 2197
BseMI	GCAATG	4 cut(s)	966, 1136, 1839, 2246
BseMII	CTCAG	3 cut(s)	85, 820, 2121
BseNI	ACTGG	6 cut(s)	58, 377, 489, 612, 1054, 1315
BseRI	GAGGAG	2 cut(s)	353, 2147
BseXI	GCAGC	3 cut(s)	255, 2317, 2503
BsgI	GTGCAG	1 cut(s)	260
Bsh1236I	CGCG	1 cut(s)	948
Bsh1285I	CGRYCG	1 cut(s)	2442
BshFI	GGCC	3 cut(s)	399, 1886, 2419
BshTI	ACCGGT	2 cut(s)	869, 1184
BshVI	ATCGAT	1 cut(s)	2372
BsiEI	CGRYCG	1 cut(s)	2442
BsiHKAI	GWGCWC	3 cut(s)	1206, 1354, 1800
BsiHKCI	CYCGRG	1 cut(s)	678
BslFI	GGGAC	5 cut(s)	46, 182, 655, 1761, 1825
BslI	CCNNNNNNNGG	8 cut(s)	135, 442, 627, 1031, 1103, 1744, 2131, 2197
BsmAI	GTCTC	2 cut(s)	1902, 2006
BsmFI	GGGAC	5 cut(s)	46, 182, 655, 1761, 1825
BsmI	GAATGC	1 cut(s)	52
BsnI	GGCC	3 cut(s)	399, 1886, 2419
Bso31I	GGTCTC	1 cut(s)	1902
BsoBI	CYCGRG	1 cut(s)	678
Bsp1286I	GDGCHC	3 cut(s)	1206, 1354, 1800
Bsp13I	TCCGGA	3 cut(s)	457, 518, 1700
Bsp1720I	GCTNAGC	1 cut(s)	71
Bsp19I	CCATGG	1 cut(s)	90
BspACI	CCGC	8 cut(s)	42, 448, 946, 1805, 1951, 2333, 2385, 2462
BspANI	GGCC	3 cut(s)	399, 1886, 2419
BspCNI	CTCAG	3 cut(s)	84, 819, 2122
BspDI	ATCGAT	1 cut(s)	2372
BspEI	TCCGGA	3 cut(s)	457, 518, 1700
BspFNI	CGCG	1 cut(s)	948
BspHI	TCATGA	2 cut(s)	204, 1942
BspLI	GGNNCC	5 cut(s)	148, 304, 625, 711, 2046
BspMI	ACCTGC	1 cut(s)	1881
BspOI	GCTAGC	2 cut(s)	504, 2024
BspPI	GGATC	2 cut(s)	935, 1016
BspTI	CTTAAG	1 cut(s)	1385
BspTNI	GGTCTC	1 cut(s)	1902
BsrDI	GCAATG	4 cut(s)	966, 1136, 1839, 2246
BsrFI	RCCGGY	2 cut(s)	869, 1184
BsrI	ACTGG	6 cut(s)	58, 377, 489, 612, 1054, 1315
BssAI	RCCGGY	2 cut(s)	869, 1184
BssECI	CCNNGG	6 cut(s)	90, 162, 528, 570, 1845, 2484
BssT1I	CCWWGG	4 cut(s)	90, 570, 1845, 2484
Bst2UI	CCWGG	3 cut(s)	163, 443, 1577
Bst4CI	ACNGT	6 cut(s)	39, 695, 1084, 1483, 1523, 1645
Bst6I	CTCTTC	1 cut(s)	1682
BstAFI	CTTAAG	1 cut(s)	1385
BstAPI	GCANNNNNTGC	1 cut(s)	283
BstC8I	GCNNGC	8 cut(s)	87, 502, 609, 1953, 2022, 2126, 2328, 2387
BstDEI	CTNAG	8 cut(s)	71, 468, 806, 1166, 1722, 1817, 2130, 2253
BstDSI	CCRYGG	1 cut(s)	90
BstEII	GGTNACC	3 cut(s)	196, 565, 983
BstF5I	GGATG	8 cut(s)	238, 1145, 1343, 1441, 1774, 1855, 2275, 2284
BstFNI	CGCG	1 cut(s)	948
BstHHI	GCGC	1 cut(s)	288
BstMAI	GTCTC	2 cut(s)	1902, 2006
BstMCI	CGRYCG	1 cut(s)	2442
BstNI	CCWGG	3 cut(s)	163, 443, 1577
BstNSI	RCATGY	2 cut(s)	1062, 1804
BstPI	GGTNACC	3 cut(s)	196, 565, 983
BstSCI	CCNGG	5 cut(s)	161, 441, 528, 626, 1575
BstUI	CGCG	1 cut(s)	948
BstV1I	GCAGC	3 cut(s)	255, 2317, 2503
BstV2I	GAAGAC	1 cut(s)	13
BstX2I	RGATCY	1 cut(s)	1234
BstXI	CCANNNNNNTGG	2 cut(s)	979, 1846
BstYI	RGATCY	1 cut(s)	1234
Bsu15I	ATCGAT	1 cut(s)	2372
Bsu36I	CCTNAGG	1 cut(s)	2130
BsuRI	GGCC	3 cut(s)	399, 1886, 2419
BsuTUI	ATCGAT	1 cut(s)	2372
BtgI	CCRYGG	1 cut(s)	90
BtgZI	GCGATG	3 cut(s)	519, 1718, 2211
BtrI	CACGTC	2 cut(s)	424, 895
BtsCI	GGATG	8 cut(s)	238, 1145, 1343, 1441, 1774, 1855, 2275, 2284
BtsI	GCAGTG	1 cut(s)	1689
BtsIMutI	CAGTG	2 cut(s)	35, 1689
BveI	ACCTGC	1 cut(s)	1881
Cac8I	GCNNGC	8 cut(s)	87, 502, 609, 1953, 2022, 2126, 2328, 2387
CciI	TCATGA	2 cut(s)	204, 1942
CfoI	GCGC	1 cut(s)	288
Cfr10I	RCCGGY	2 cut(s)	869, 1184
Cfr13I	GGNCC	6 cut(s)	79, 302, 710, 851, 1529, 1726
ClaI	ATCGAT	1 cut(s)	2372
CseI	GACGC	3 cut(s)	260, 780, 1822
Csp6I	GTAC	7 cut(s)	159, 930, 1038, 1116, 1265, 2219, 2598
CspAI	ACCGGT	2 cut(s)	869, 1184
CviQI	GTAC	7 cut(s)	159, 930, 1038, 1116, 1265, 2219, 2598
DdeI	CTNAG	8 cut(s)	71, 468, 806, 1166, 1722, 1817, 2130, 2253
DraIII	CACNNNGTG	2 cut(s)	1744, 2149
EaeI	YGGCCR	1 cut(s)	1884
Eam1104I	CTCTTC	1 cut(s)	1682
EarI	CTCTTC	1 cut(s)	1682
Ecl136II	GAGCTC	1 cut(s)	1204
Eco130I	CCWWGG	4 cut(s)	90, 570, 1845, 2484
Eco24I	GRGCYC	1 cut(s)	1206
Eco31I	GGTCTC	1 cut(s)	1902
Eco47I	GGWCC	6 cut(s)	79, 302, 710, 851, 1529, 1726
Eco53kI	GAGCTC	1 cut(s)	1204
Eco57I	CTGAAG	1 cut(s)	800
Eco81I	CCTNAGG	1 cut(s)	2130
Eco88I	CYCGRG	1 cut(s)	678
Eco91I	GGTNACC	3 cut(s)	196, 565, 983
EcoICRI	GAGCTC	1 cut(s)	1204
EcoO109I	RGGNCCY	2 cut(s)	710, 851
EcoO65I	GGTNACC	3 cut(s)	196, 565, 983
EcoRI	GAATTC	1 cut(s)	416
EcoRII	CCWGG	3 cut(s)	161, 441, 1575
EcoT14I	CCWWGG	4 cut(s)	90, 570, 1845, 2484
EcoT38I	GRGCYC	1 cut(s)	1206
ErhI	CCWWGG	4 cut(s)	90, 570, 1845, 2484
FaqI	GGGAC	5 cut(s)	46, 182, 655, 1761, 1825
FauI	CCCGC	3 cut(s)	953, 1944, 2378
FbaI	TGATCA	2 cut(s)	207, 1459
FblI	GTMKAC	2 cut(s)	426, 1822
Fnu4HI	GCNGC	5 cut(s)	269, 449, 1805, 2331, 2517
FokI	GGATG	8 cut(s)	225, 1132, 1350, 1428, 1781, 1862, 2282, 2291
FriOI	GRGCYC	1 cut(s)	1206
Fsp4HI	GCNGC	5 cut(s)	269, 449, 1805, 2331, 2517
FspBI	CTAG	7 cut(s)	501, 558, 1269, 2021, 2147, 2382, 2625
FspI	TGCGCA	1 cut(s)	287
GlaI	GCGC	1 cut(s)	287
GluI	GCNGC	5 cut(s)	269, 449, 1805, 2331, 2517
GsuI	CTGGAG	2 cut(s)	1974, 2031
HaeIII	GGCC	3 cut(s)	399, 1886, 2419
HgaI	GACGC	3 cut(s)	260, 780, 1822
HhaI	GCGC	1 cut(s)	288
Hin6I	GCGC	1 cut(s)	286
HinP1I	GCGC	1 cut(s)	286
HincII	GTYRAC	2 cut(s)	427, 1294
HindII	GTYRAC	2 cut(s)	427, 1294
HinfI	GANTC	7 cut(s)	404, 454, 631, 676, 1697, 2357, 2435
HpaI	GTTAAC	1 cut(s)	1294
HphI	GGTGA	6 cut(s)	190, 289, 611, 859, 1469, 2135
Hpy166II	GTNNAC	6 cut(s)	427, 892, 1294, 1823, 2065, 2446
Hpy188I	TCNGA	9 cut(s)	151, 730, 819, 1110, 1730, 2075, 2456, 2548, 2577
Hpy8I	GTNNAC	6 cut(s)	427, 892, 1294, 1823, 2065, 2446
Hpy99I	CGWCG	1 cut(s)	428
HpyAV	CCTTC	7 cut(s)	316, 419, 448, 590, 1646, 1929, 2417
HpyCH4III	ACNGT	6 cut(s)	39, 695, 1084, 1483, 1523, 1645
HpyCH4IV	ACGT	4 cut(s)	171, 176, 423, 894
HpyF3I	CTNAG	8 cut(s)	71, 468, 806, 1166, 1722, 1817, 2130, 2253
HpySE526I	ACGT	4 cut(s)	171, 176, 423, 894
HspAI	GCGC	1 cut(s)	286
Kpn2I	TCCGGA	3 cut(s)	457, 518, 1700
Ksp22I	TGATCA	2 cut(s)	207, 1459
KspAI	GTTAAC	1 cut(s)	1294
LmnI	GCTCC	4 cut(s)	152, 1349, 1993, 2050
Lsp1109I	GCAGC	3 cut(s)	255, 2317, 2503
LweI	GCATC	4 cut(s)	496, 913, 2173, 2269
MaeI	CTAG	7 cut(s)	501, 558, 1269, 2021, 2147, 2382, 2625
MaeII	ACGT	4 cut(s)	171, 176, 423, 894
MfeI	CAATTG	1 cut(s)	2112
MflI	RGATCY	1 cut(s)	1234
MhlI	GDGCHC	3 cut(s)	1206, 1354, 1800
MlsI	TGGCCA	1 cut(s)	1886
MluNI	TGGCCA	1 cut(s)	1886
MlyI	GAGTC	2 cut(s)	670, 2444
MmeI	TCCRAC	2 cut(s)	1327, 1753
Mox20I	TGGCCA	1 cut(s)	1886
MroI	TCCGGA	3 cut(s)	457, 518, 1700
MroXI	GAANNNNTTC	2 cut(s)	99, 362
MscI	TGGCCA	1 cut(s)	1886
MslI	CAYNNNNRTG	2 cut(s)	203, 1455
Msp20I	TGGCCA	1 cut(s)	1886
MspCI	CTTAAG	1 cut(s)	1385
MspR9I	CCNGG	5 cut(s)	163, 443, 530, 628, 1577
MunI	CAATTG	1 cut(s)	2112
Mva1269I	GAATGC	1 cut(s)	52
MvaI	CCWGG	3 cut(s)	163, 443, 1577
MvnI	CGCG	1 cut(s)	948
NciI	CCSGG	2 cut(s)	530, 628
NcoI	CCATGG	1 cut(s)	90
NheI	GCTAGC	2 cut(s)	500, 2020
NlaIV	GGNNCC	5 cut(s)	148, 304, 625, 711, 2046
NmuCI	GTSAC	9 cut(s)	33, 196, 895, 949, 1448, 1591, 2095, 2141, 2149
NsbI	TGCGCA	1 cut(s)	287
NspI	RCATGY	2 cut(s)	1062, 1804
OliI	CACNNNNGTG	1 cut(s)	1455
PaeR7I	CTCGAG	1 cut(s)	678
PagI	TCATGA	2 cut(s)	204, 1942
PciI	ACATGT	1 cut(s)	1058
PcsI	WCGNNNNNNNCGW	2 cut(s)	137, 177
PctI	GAATGC	1 cut(s)	52
PdmI	GAANNNNTTC	2 cut(s)	99, 362
PfeI	GAWTC	5 cut(s)	404, 454, 631, 1697, 2357
PflMI	CCANNNNNTGG	2 cut(s)	135, 1744
PfoI	TCCNGGA	1 cut(s)	626
PinAI	ACCGGT	2 cut(s)	869, 1184
PkrI	GCNGC	5 cut(s)	270, 450, 1806, 2332, 2518
Ple19I	CGATCG	1 cut(s)	2442
PleI	GAGTC	2 cut(s)	670, 2443
PpsI	GAGTC	2 cut(s)	670, 2443
PpuMI	RGGWCCY	2 cut(s)	710, 851
PscI	ACATGT	1 cut(s)	1058
PshBI	ATTAAT	1 cut(s)	2084
PsiI	TTATAA	1 cut(s)	585
Psp124BI	GAGCTC	1 cut(s)	1206
Psp5II	RGGWCCY	2 cut(s)	710, 851
Psp6I	CCWGG	3 cut(s)	161, 441, 1575
PspEI	GGTNACC	3 cut(s)	196, 565, 983
PspGI	CCWGG	3 cut(s)	161, 441, 1575
PspN4I	GGNNCC	5 cut(s)	148, 304, 625, 711, 2046
PspPI	GGNCC	6 cut(s)	79, 302, 710, 851, 1529, 1726
PspPPI	RGGWCCY	2 cut(s)	710, 851
PspXI	VCTCGAGB	1 cut(s)	678
PsuI	RGATCY	1 cut(s)	1234
PvuI	CGATCG	1 cut(s)	2442
RsaI	GTAC	7 cut(s)	160, 931, 1039, 1117, 1266, 2220, 2599
RsaNI	GTAC	7 cut(s)	159, 930, 1038, 1116, 1265, 2219, 2598
RseI	CAYNNNNRTG	2 cut(s)	203, 1455
SacI	GAGCTC	1 cut(s)	1206
SalI	GTCGAC	1 cut(s)	425
SatI	GCNGC	5 cut(s)	269, 449, 1805, 2331, 2517
Sau96I	GGNCC	6 cut(s)	79, 302, 710, 851, 1529, 1726
ScaI	AGTACT	1 cut(s)	1039
SchI	GAGTC	2 cut(s)	670, 2444
ScrFI	CCNGG	5 cut(s)	163, 443, 530, 628, 1577
SduI	GDGCHC	3 cut(s)	1206, 1354, 1800
SfaNI	GCATC	4 cut(s)	496, 913, 2173, 2269
Sfr274I	CTCGAG	1 cut(s)	678
SgrAI	CRCCGGYG	1 cut(s)	869
SinI	GGWCC	6 cut(s)	79, 302, 710, 851, 1529, 1726
SlaI	CTCGAG	1 cut(s)	678
SmiMI	CAYNNNNRTG	2 cut(s)	203, 1455
SmlI	CTYRAG	3 cut(s)	335, 678, 1385
SmoI	CTYRAG	3 cut(s)	335, 678, 1385
SsiI	CCGC	8 cut(s)	42, 448, 946, 1805, 1951, 2333, 2385, 2462
SspI	AATATT	2 cut(s)	1927, 2082
SspMI	CTAG	7 cut(s)	501, 558, 1269, 2021, 2147, 2382, 2625
SstI	GAGCTC	1 cut(s)	1206
StyD4I	CCNGG	5 cut(s)	161, 441, 528, 626, 1575
StyI	CCWWGG	4 cut(s)	90, 570, 1845, 2484
TaaI	ACNGT	6 cut(s)	39, 695, 1084, 1483, 1523, 1645
TaiI	ACGT	4 cut(s)	174, 179, 426, 897
TaqI	TCGA	9 cut(s)	105, 402, 426, 594, 679, 1276, 2292, 2372, 2438
TatI	WGTACW	2 cut(s)	1037, 2218
TauI	GCSGC	2 cut(s)	451, 1807
TfiI	GAWTC	5 cut(s)	404, 454, 631, 1697, 2357
TscAI	CASTG	2 cut(s)	42, 1689
TseFI	GTSAC	9 cut(s)	33, 196, 895, 949, 1448, 1591, 2095, 2141, 2149
TseI	GCWGC	3 cut(s)	268, 2330, 2516
Tsp45I	GTSAC	9 cut(s)	33, 196, 895, 949, 1448, 1591, 2095, 2141, 2149
TspGWI	ACGGA	1 cut(s)	1542
TspRI	CASTG	2 cut(s)	42, 1689
Van91I	CCANNNNNTGG	2 cut(s)	135, 1744
Vha464I	CTTAAG	1 cut(s)	1385
VpaK11BI	GGWCC	6 cut(s)	79, 302, 710, 851, 1529, 1726
VspI	ATTAAT	1 cut(s)	2084
XapI	RAATTY	6 cut(s)	416, 533, 722, 754, 1210, 1426
XceI	RCATGY	2 cut(s)	1062, 1804
XcmI	CCANNNNNNNNNTGG	1 cut(s)	132
XhoI	CTCGAG	1 cut(s)	678
XmiI	GTMKAC	2 cut(s)	426, 1822
XmnI	GAANNNNTTC	2 cut(s)	99, 362
XspI	CTAG	7 cut(s)	501, 558, 1269, 2021, 2147, 2382, 2625
ZrmI	AGTACT	1 cut(s)	1039

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.