Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0003113

Physical Location & Seq

Genomic Coordinates Forward (+)

1180425 .. 1185052

Gene Structure 4628 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1015200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2829 bp

ATGAAGGAACCCCAACTGGGTTTCTGGGGTTCTTTCCCCGTTCTGCTGCTTTTGTTTTTCTCGCTGTACCCACCAGTACACTCCCAGTCCGGCGGTGGCGGCGACGACGGAGGGGCCATGGAAGCACTCCGGAAAAGCATCGGCTCCAACAATCTCGGGTGGTCCGGCACCGATTACTGCAAATGGGGAAAAGTGAGTTGCCAGGCCGGCAAGGTTTTCAAAATCCAATTGGGTAATCAGAAACTCACCGGCACGCTGCCGCCGGAAATCCAAAAGCTTTCGAATTTGCAGCAACTGGAAGTTCAGAACAACCAACTCACCGGACCTTTTCCGAGCCTCTCCGGGGTGCAACCGCTTCAGGTCCTCCTCGCCCACGACAACAACTTCTCGTCCTTCCCCTCCGATTTCTTCACCGGACTCACCTCCCTCGTCAACATCAACATCGACCACAATCCCTTCGCTGCGTGGCAGATTCCCAACACTCTCAAAGGTGCCACACAGCTGAAGGAATTCTCCGCCACCGAGACCAACATCACCGGAAGAATCCCCGATATCTTCAGCAGTTCCAATTTCCCAGGTTTGACTGATCTACATTTGGCTTTCAATTACCTTGAAGGCGAATTGCCTGCTAGTTTTTCGAGTTCGAGTATTCAGTCCCTCTGGTTGAACAGCCAACAGGGCACCAATAGGCTTAATGGTACTATTGATGTGTTACAGAACATGACTTCTCTGCGTGAGGTTTGGTTACATGGTAATTATTTCACCGGTCCTATACCGGACCTGTCGAACTTAGGTTACTTGACCACTCTGAGTTTGAGGGATAACAAGCTCACCGGCGTTGTTCCCGCGTCTTTGTTGAATCTTAAATCCCTTACTTCTGTTAATTTGACCAATAATATGCTTCAAGGACCAATGCCCAAGTTTGGCGATGGGGTTCTGGTGGATATGTCGGGGTTAAATAGTTTTTGCAGTGATAAGCCGGGTGTTGATTGCGACGCTCGTGTCAATGTATTGCTTTCGGTCATCAAAGACATGGGTTATCCTACTGTTTTTGCAGATAGTTGGAAGGGGAACGATTCTTGTGATAACTGGAAGGGAATTACGTGTAATGGTGGAAACATCACTGTTGTCAATTTTCGGAGCCTGGGTCTTTCGGGTACGATCTCTTCAAATTATTCTCTGCTTACCTCCTTGCAGACATTGAGACTTGACAATAATAACCTCACGGGTACAATACCAAAGGAGCTTACGCAACTGCCCAACCTTCAACAGATAGATGTTAGTAACAATCAACTTTTTGGTCAAGTACCAAAGTTTAAGAATGTGGACGTGAAAACTGATGGGAACCCTAATATTGGTCAGGATCATCCCCCTTCGCCAGCGACGCCTACAATTCCTAATTCACCGCCTGCCTCCCCCTCAGATGGAGGTAAAAAATCTAGGAGTGGGGTGGTTGTTGGGGCTGTCATCGGCACCGTTGGCGGATTGGTTGTAGTTGGGTTTGTTGCTTTCTGTCTACTTAAGAAAAAGCATAAGCATTCTGGCAGAGTACAAAGTCCAAACGCATTGGTTATTCATCCTCGTCACTCTGGCGATCAAAATGCAGTCAAGATCACGGTTGCTAACTCTGGGGTTAATCGCGGTGAAAATGAGTCCTATAATAGTCCGGCCAACAGTGGACCTAATGACATTCATGTGGTTGAGGCTGGAAGTATGGTCATTTCGATCCAAGTTTTGAGAGATGTGACCAATAATTTCAGCGAAAATAATGTATTAGGAAAAGGTGGTTTTGGAACTGTGTACAAAGGGGAGTTGCATGATGGGACAAAGATTGCAGTGAAGAGGATGGAATCTGGAGTAGTGGCGGAGAAGGGTCTAAATGAGTTCAAGTCTGAGATTGCAGTTCTTACTAAAGTCCGACACCGCCACTTGGTTGGACTTCTTGGCTATTGTTTGGACGGAAACGAGAGGCTTCTTGTTTATGAATACATGCCTCAAGGGACTCTTAGTAGATACTTGTTCAACTGGAAAGAGGAAGGTCTGAAGCCACTTGAATGGACTAGAAGGCTGACCATTGCCTTGGATGTTGCAAGAGGGGTTGAGTATCTCCACGGTTTAGCCAGCCAGACTTTCATTCACAGGGATCTTAAACCTTCGAACATTTTACTCGGAGATGATATGCGGGCTAAAGTTTCAGATTTTGGACTGGTTCGTCTTGCTCCAGAAGGGAAAGCCTCAATTGAGACGAGACTAGCCGGAACTTTTGGCTATTTGGCTCCAGAGTATGCAGCAACTGGGCGGATGACACTCAAGGTTGACGTGTATAGCTTTGGAGTGATCTTAATGGAGCTGATCACGGGTAGAAGAGCAATTGATGAAAGCCAACCAGAGGAGAGCTTGCACCTTGTTACATGGTTCCGCAGAATGCTCATTAACAAGGATGCATTCCGGAAGGCCATTGATCCAACAATTGATCTCAATGAGGAAACTCTTTCTAGTATTAACACCGTCGCTGAGCTTGCTGGGCATTGCAGCGCAAGGGAGCCCTACCAGAGGCCCGACATGGGTCATGCAGTCAATGTTCTTTCGTCGCTCGTTGAGCATTGGAAACCATCTGAAGCCGAAGATTCTGATGATATGTATGGAATTGACCTCGAAATGACCTTACCACAAGCACTAAAGAAGTGGCAGGCTTTTGAAGGCAATAGCAACCTTGATGGGTCTTCATCTTCTTCATCGTTCTTTGCCAGCGGGGACAACACCCAAACTAGCATACCTACTCGGCCATCTGGATTTGCGGAATCGTTTACATCATTGGATGGGCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005576 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

943

Amino Acids

102.38

Weight (kDa)

6.09

Isoelectric Point (pI)

35.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_4	PF12799	396 - 437	1.5e-06	Leucine Rich repeats (2 copies)
Pkinase	PF00069	587 - 859	1.4e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	589 - 813	5.5e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003113)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01820
fragaria_vesca	FvH4_4g33170 FvH4_4g33170 FvH4_4g33171
malus_domestica	MD13G1017200.v1.1 MD16G1015000.v1.1 MD16G1015200.v1.1
prunus_persica	Prupe.1G336300_v2.0.a1 Prupe.1G336300_v2.0.a1
pyrus_communis	pycom13g01480 pycom16g01400
rosa_chinensis	RchiOBHm_Chr4g0442211 RchiOBHm_Chr4g0442221 RchiOBHm_Chr4g0442231
rosa_laevigata	RLG00000006022 RLG00000006023
rosa_multiflora	Rmu_co8222306.1_g000001 Rmu_sc0000124.1_g000060 Rmu_sc0000124.1_g000062 Rmu_sc0010284.1_g000002 Rmu_sc0010284.1_g000003
rosa_roxburghii	Rroxscaffold_5G00383140 Rroxscaffold_5G00383150
rosa_rugosa	Rorug04G0339700 Rorug04G0339800 Rorug04G0339900
rosa_samantha	Rh4AG391300 Rh4AG391400 Rh4BG405000 Rh4BG405100 Rh4CG420100 Rh4CG420200 Rh4DG398100 Rh4DG398200 Rh4DG398300
rosa_wichuraiana	Rw4G033820 Rw4G033830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1001, 2211
AccB1I	GGYRCC	4 cut(s)	167, 491, 680, 1472
AccI	GTMKAC	1 cut(s)	1516
AccII	CGCG	2 cut(s)	848, 1641
AccIII	TCCGGA	2 cut(s)	129, 2448
AclWI	GGATC	4 cut(s)	1371, 1720, 2151, 2456
AcoI	YGGCCR	2 cut(s)	1668, 2783
AcsI	RAATTY	2 cut(s)	283, 509
AcuI	CTGAAG	5 cut(s)	341, 524, 541, 2063, 2637
AcyI	GRCGYC	1 cut(s)	1385
AfaI	GTAC	8 cut(s)	68, 78, 700, 1159, 1231, 1308, 1551, 1802
AfiI	CCNNNNNNNGG	9 cut(s)	17, 343, 923, 1355, 1424, 1930, 2389, 2553, 2564
AflII	CTTAAG	1 cut(s)	1520
AflIII	ACRYGT	2 cut(s)	1103, 2319
AgeI	ACCGGT	1 cut(s)	764
AjiI	CACGTC	2 cut(s)	1330, 2320
AjnI	CCWGG	3 cut(s)	201, 574, 1143
AluBI	AGCT	8 cut(s)	277, 502, 829, 1246, 2328, 2350, 2397, 2518
AluI	AGCT	8 cut(s)	277, 502, 829, 1246, 2328, 2350, 2397, 2518
Alw26I	GTCTC	4 cut(s)	518, 1198, 2237, 2242
AlwI	GGATC	4 cut(s)	1371, 1720, 2151, 2456
AlwNI	CAGNNNCTG	2 cut(s)	295, 2294
Ama87I	CYCGRG	1 cut(s)	155
Aor13HI	TCCGGA	2 cut(s)	129, 2448
AoxI	GGCC	6 cut(s)	114, 204, 1668, 2454, 2555, 2783
ApeKI	GCWGC	6 cut(s)	46, 256, 289, 461, 2288, 2532
ApoI	RAATTY	2 cut(s)	283, 509
AsiGI	ACCGGT	1 cut(s)	764
Asp700I	GAANNNNTTC	1 cut(s)	509
AspLEI	GCGC	1 cut(s)	2537
AspS9I	GGNCC	9 cut(s)	114, 162, 323, 361, 767, 778, 908, 1679, 2556
AsuC2I	CCSGG	2 cut(s)	343, 981
AsuHPI	GGTGA	9 cut(s)	238, 310, 403, 412, 526, 754, 823, 1395, 1655
AsuII	TTCGAA	2 cut(s)	281, 2156
AvaI	CYCGRG	1 cut(s)	155
AvaII	GGWCC	7 cut(s)	162, 323, 361, 767, 778, 908, 1679
BaeGI	GKGCMC	1 cut(s)	683
BanI	GGYRCC	4 cut(s)	167, 491, 680, 1472
BanII	GRGCYC	1 cut(s)	2547
BarI	GAAGNNNNNNTAC	2 cut(s)	1150, 1182
BauI	CACGAG	1 cut(s)	999
BbsI	GAAGAC	1 cut(s)	2715
BbvI	GCAGC	6 cut(s)	33, 243, 301, 448, 2300, 2544
BccI	CCATC	9 cut(s)	923, 1334, 1418, 1814, 1840, 2620, 2711, 2794, 2813
BcgI	CGANNNNNNTGC	4 cut(s)	608, 642, 1583, 1617
BciT130I	CCWGG	3 cut(s)	203, 576, 1145
BclI	TGATCA	1 cut(s)	2352
BcnI	CCSGG	2 cut(s)	343, 981
BcoDI	GTCTC	4 cut(s)	518, 1198, 2237, 2242
BfaI	CTAG	6 cut(s)	630, 1440, 2061, 2252, 2496, 2769
BfrI	CTTAAG	1 cut(s)	1520
BglI	GCCNNNNNGGC	2 cut(s)	207, 678
BisI	GCNGC	8 cut(s)	47, 100, 257, 260, 290, 462, 2289, 2533
BlpI	GCTNAGC	1 cut(s)	2514
BlsI	GCNGC	8 cut(s)	48, 101, 258, 261, 291, 463, 2290, 2534
Bme1390I	CCNGG	5 cut(s)	203, 343, 576, 981, 1145
Bme18I	GGWCC	7 cut(s)	162, 323, 361, 767, 778, 908, 1679
BmeT110I	CYCGRG	1 cut(s)	155
BmgBI	CACGTC	2 cut(s)	1330, 2320
BmgT120I	GGNCC	9 cut(s)	114, 162, 323, 361, 767, 778, 908, 1679, 2556
BmrFI	CCNGG	5 cut(s)	203, 343, 576, 981, 1145
BmrI	ACTGGG	3 cut(s)	26, 79, 2304
BmsI	GCATC	2 cut(s)	147, 2431
BmuI	ACTGGG	3 cut(s)	26, 79, 2304
BoxI	GACNNNNGTC	1 cut(s)	2565
BpiI	GAAGAC	1 cut(s)	2715
BpmI	CTGGAG	3 cut(s)	1875, 2205, 2262
Bpu1102I	GCTNAGC	1 cut(s)	2514
Bpu14I	TTCGAA	2 cut(s)	281, 2156
BpuEI	CTTGAG	2 cut(s)	1980, 2294
BpuMI	CCSGG	2 cut(s)	343, 981
BsaAI	YACGTR	1 cut(s)	1104
BsaHI	GRCGYC	1 cut(s)	1385
BsaI	GGTCTC	1 cut(s)	518
BsaJI	CCNNGG	6 cut(s)	117, 342, 574, 1144, 2079, 2110
BsaWI	WCCGGW	7 cut(s)	129, 320, 413, 536, 764, 775, 2448
BsaXI	ACNNNNNCTCC	2 cut(s)	1132, 1162
Bsc4I	CCNNNNNNNGG	9 cut(s)	17, 343, 923, 1355, 1424, 1930, 2389, 2553, 2564
Bse118I	RCCGGY	4 cut(s)	206, 248, 764, 833
Bse1I	ACTGG	8 cut(s)	21, 74, 85, 300, 1094, 2030, 2211, 2299
Bse3DI	GCAATG	2 cut(s)	2073, 2527
BseAI	TCCGGA	2 cut(s)	129, 2448
BseBI	CCWGG	3 cut(s)	203, 576, 1145
BseDI	CCNNGG	6 cut(s)	117, 342, 574, 1144, 2079, 2110
BseGI	GGATG	7 cut(s)	1366, 1576, 1851, 2089, 2307, 2446, 2824
BseLI	CCNNNNNNNGG	9 cut(s)	17, 343, 923, 1355, 1424, 1930, 2389, 2553, 2564
BseMI	GCAATG	2 cut(s)	2073, 2527
BseMII	CTCAG	4 cut(s)	800, 1434, 1884, 2505
BseNI	ACTGG	8 cut(s)	21, 74, 85, 300, 1094, 2030, 2211, 2299
BseRI	GAGGAG	2 cut(s)	356, 2405
BseSI	GKGCMC	1 cut(s)	683
BseXI	GCAGC	6 cut(s)	33, 243, 301, 448, 2300, 2544
BseYI	CCCAGC	1 cut(s)	2522
Bsh1236I	CGCG	2 cut(s)	848, 1641
BshFI	GGCC	6 cut(s)	116, 206, 1670, 2456, 2557, 2785
BshNI	GGYRCC	4 cut(s)	167, 491, 680, 1472
BshTI	ACCGGT	1 cut(s)	764
BsiHKCI	CYCGRG	1 cut(s)	155
BslFI	GGGAC	4 cut(s)	640, 1837, 2014, 2768
BslI	CCNNNNNNNGG	9 cut(s)	17, 343, 923, 1355, 1424, 1930, 2389, 2553, 2564
BsmAI	GTCTC	4 cut(s)	518, 1198, 2237, 2242
BsmBI	CGTCTC	1 cut(s)	2237
BsmFI	GGGAC	4 cut(s)	640, 1837, 2014, 2768
BsmI	GAATGC	3 cut(s)	1537, 2430, 2444
BsnI	GGCC	6 cut(s)	116, 206, 1670, 2456, 2557, 2785
Bso31I	GGTCTC	1 cut(s)	518
BsoBI	CYCGRG	1 cut(s)	155
Bsp119I	TTCGAA	2 cut(s)	281, 2156
Bsp1286I	GDGCHC	2 cut(s)	683, 2547
Bsp13I	TCCGGA	2 cut(s)	129, 2448
Bsp1407I	TGTACA	1 cut(s)	1800
Bsp1720I	GCTNAGC	1 cut(s)	2514
Bsp19I	CCATGG	1 cut(s)	117
BspANI	GGCC	6 cut(s)	116, 206, 1670, 2456, 2557, 2785
BspCNI	CTCAG	4 cut(s)	801, 1433, 1885, 2506
BspEI	TCCGGA	2 cut(s)	129, 2448
BspFNI	CGCG	2 cut(s)	848, 1641
BspPI	GGATC	4 cut(s)	1371, 1720, 2151, 2456
BspQI	GCTCTTC	1 cut(s)	2359
BspT104I	TTCGAA	2 cut(s)	281, 2156
BspT107I	GGYRCC	4 cut(s)	167, 491, 680, 1472
BspTI	CTTAAG	1 cut(s)	1520
BspTNI	GGTCTC	1 cut(s)	518
BsrDI	GCAATG	2 cut(s)	2073, 2527
BsrFI	RCCGGY	4 cut(s)	206, 248, 764, 833
BsrGI	TGTACA	1 cut(s)	1800
BsrI	ACTGG	8 cut(s)	21, 74, 85, 300, 1094, 2030, 2211, 2299
BssAI	RCCGGY	4 cut(s)	206, 248, 764, 833
BssECI	CCNNGG	6 cut(s)	117, 342, 574, 1144, 2079, 2110
BssNI	GRCGYC	1 cut(s)	1385
BssSI	CACGAG	1 cut(s)	999
BssT1I	CCWWGG	2 cut(s)	117, 2079
Bst2BI	CACGAG	1 cut(s)	999
Bst2UI	CCWGG	3 cut(s)	203, 576, 1145
Bst4CI	ACNGT	8 cut(s)	1048, 1126, 1477, 1618, 1676, 1798, 2114, 2509
Bst6I	CTCTTC	3 cut(s)	1171, 1835, 2359
BstACI	GRCGYC	1 cut(s)	1385
BstAFI	CTTAAG	1 cut(s)	1520
BstAUI	TGTACA	1 cut(s)	1800
BstBAI	YACGTR	1 cut(s)	1104
BstBI	TTCGAA	2 cut(s)	281, 2156
BstDEI	CTNAG	6 cut(s)	790, 809, 1420, 1893, 2006, 2514
BstDSI	CCRYGG	2 cut(s)	117, 2110
BstENI	CCTNNNNNAGG	1 cut(s)	2551
BstF5I	GGATG	7 cut(s)	1366, 1576, 1851, 2089, 2307, 2446, 2824
BstFNI	CGCG	2 cut(s)	848, 1641
BstHHI	GCGC	1 cut(s)	2537
BstMAI	GTCTC	4 cut(s)	518, 1198, 2237, 2242
BstMWI	GCNNNNNNNGC	9 cut(s)	99, 122, 207, 678, 1384, 1479, 2519, 2524, 2599
BstNI	CCWGG	3 cut(s)	203, 576, 1145
BstNSI	RCATGY	1 cut(s)	1993
BstPAI	GACNNNNGTC	1 cut(s)	2565
BstSCI	CCNGG	5 cut(s)	201, 341, 574, 979, 1143
BstSLI	GKGCMC	1 cut(s)	683
BstUI	CGCG	2 cut(s)	848, 1641
BstV1I	GCAGC	6 cut(s)	33, 243, 301, 448, 2300, 2544
BstV2I	GAAGAC	1 cut(s)	2715
BstX2I	RGATCY	1 cut(s)	2143
BstXI	CCANNNNNNTGG	3 cut(s)	1934, 2055, 2080
BstYI	RGATCY	1 cut(s)	2143
BsuRI	GGCC	6 cut(s)	116, 206, 1670, 2456, 2557, 2785
BtgI	CCRYGG	2 cut(s)	117, 2110
BtgZI	GCGATG	1 cut(s)	942
BtrI	CACGTC	2 cut(s)	1330, 2320
BtsCI	GGATG	7 cut(s)	1366, 1576, 1851, 2089, 2307, 2446, 2824
BtsI	GCAGTG	2 cut(s)	976, 1842
BtsIMutI	CAGTG	4 cut(s)	976, 1122, 1681, 1842
CaiI	CAGNNNCTG	2 cut(s)	295, 2294
CfoI	GCGC	1 cut(s)	2537
Cfr10I	RCCGGY	4 cut(s)	206, 248, 764, 833
Cfr13I	GGNCC	9 cut(s)	114, 162, 323, 361, 767, 778, 908, 1679, 2556
CseI	GACGC	3 cut(s)	837, 1004, 1393
Csp6I	GTAC	8 cut(s)	67, 77, 699, 1158, 1230, 1307, 1550, 1801
CspAI	ACCGGT	1 cut(s)	764
CviQI	GTAC	8 cut(s)	67, 77, 699, 1158, 1230, 1307, 1550, 1801
DdeI	CTNAG	6 cut(s)	790, 809, 1420, 1893, 2006, 2514
DrdI	GACNNNNNNGTC	2 cut(s)	1001, 2211
DseDI	GACNNNNNNGTC	2 cut(s)	1001, 2211
EaeI	YGGCCR	2 cut(s)	1668, 2783
Eam1104I	CTCTTC	3 cut(s)	1171, 1835, 2359
EarI	CTCTTC	3 cut(s)	1171, 1835, 2359
EciI	GGCGGA	4 cut(s)	505, 1497, 1880, 2314
Eco130I	CCWWGG	2 cut(s)	117, 2079
Eco24I	GRGCYC	1 cut(s)	2547
Eco31I	GGTCTC	1 cut(s)	518
Eco32I	GATATC	1 cut(s)	553
Eco47I	GGWCC	7 cut(s)	162, 323, 361, 767, 778, 908, 1679
Eco57I	CTGAAG	5 cut(s)	341, 524, 541, 2063, 2637
Eco88I	CYCGRG	1 cut(s)	155
EcoNI	CCTNNNNNAGG	1 cut(s)	2551
EcoO109I	RGGNCCY	1 cut(s)	361
EcoRI	GAATTC	1 cut(s)	509
EcoRII	CCWGG	3 cut(s)	201, 574, 1143
EcoRV	GATATC	1 cut(s)	553
EcoT14I	CCWWGG	2 cut(s)	117, 2079
EcoT22I	ATGCAT	1 cut(s)	2446
EcoT38I	GRGCYC	1 cut(s)	2547
ErhI	CCWWGG	2 cut(s)	117, 2079
Esp3I	CGTCTC	1 cut(s)	2237
FalI	AAGNNNNNCTT	2 cut(s)	1989, 2021
FaqI	GGGAC	4 cut(s)	640, 1837, 2014, 2768
FauI	CCCGC	3 cut(s)	853, 2175, 2744
FbaI	TGATCA	1 cut(s)	2352
FblI	GTMKAC	1 cut(s)	1516
Fnu4HI	GCNGC	8 cut(s)	47, 100, 257, 260, 290, 462, 2289, 2533
FokI	GGATG	6 cut(s)	1353, 1563, 1858, 2096, 2314, 2453
FriOI	GRGCYC	1 cut(s)	2547
Fsp4HI	GCNGC	8 cut(s)	47, 100, 257, 260, 290, 462, 2289, 2533
FspBI	CTAG	6 cut(s)	630, 1440, 2061, 2252, 2496, 2769
GlaI	GCGC	1 cut(s)	2536
GluI	GCNGC	8 cut(s)	47, 100, 257, 260, 290, 462, 2289, 2533
GsaI	CCCAGC	1 cut(s)	2526
GsuI	CTGGAG	3 cut(s)	1875, 2205, 2262
HaeIII	GGCC	6 cut(s)	116, 206, 1670, 2456, 2557, 2785
HgaI	GACGC	3 cut(s)	837, 1004, 1393
HhaI	GCGC	1 cut(s)	2537
Hin1I	GRCGYC	1 cut(s)	1385
Hin6I	GCGC	1 cut(s)	2535
HinP1I	GCGC	1 cut(s)	2535
HincII	GTYRAC	2 cut(s)	433, 2317
HindII	GTYRAC	2 cut(s)	433, 2317
HindIII	AAGCTT	1 cut(s)	275
HphI	GGTGA	9 cut(s)	238, 310, 403, 412, 526, 754, 823, 1395, 1655
Hpy166II	GTNNAC	8 cut(s)	79, 433, 1327, 1517, 1679, 1801, 2317, 2808
Hpy188III	TCNNGA	8 cut(s)	130, 1361, 1609, 1854, 2222, 2279, 2449, 2790
Hpy8I	GTNNAC	8 cut(s)	79, 433, 1327, 1517, 1679, 1801, 2317, 2808
Hpy99I	CGWCG	6 cut(s)	107, 110, 998, 1387, 2513, 2593
HpyCH4III	ACNGT	8 cut(s)	1048, 1126, 1477, 1618, 1676, 1798, 2114, 2509
HpyCH4IV	ACGT	3 cut(s)	1103, 1329, 2319
HpyF10VI	GCNNNNNNNGC	9 cut(s)	99, 122, 207, 678, 1384, 1479, 2519, 2524, 2599
HpyF3I	CTNAG	6 cut(s)	790, 809, 1420, 1893, 2006, 2514
HpySE526I	ACGT	3 cut(s)	1103, 1329, 2319
Hsp92I	GRCGYC	1 cut(s)	1385
HspAI	GCGC	1 cut(s)	2535
Kpn2I	TCCGGA	2 cut(s)	129, 2448
KroI	GCCGGC	1 cut(s)	206
KroNI	GCCGGC	1 cut(s)	208
Ksp22I	TGATCA	1 cut(s)	2352
LguI	GCTCTTC	1 cut(s)	2359
LmnI	GCTCC	7 cut(s)	149, 1140, 1243, 2224, 2281, 2347, 2542
Lsp1109I	GCAGC	6 cut(s)	33, 243, 301, 448, 2300, 2544
LweI	GCATC	2 cut(s)	147, 2431
MaeI	CTAG	6 cut(s)	630, 1440, 2061, 2252, 2496, 2769
MaeII	ACGT	3 cut(s)	1103, 1329, 2319
MaeIII	GTNAC	7 cut(s)	711, 744, 794, 1283, 1583, 1744, 2407
MfeI	CAATTG	4 cut(s)	227, 2238, 2370, 2469
MflI	RGATCY	1 cut(s)	2143
MhlI	GDGCHC	2 cut(s)	683, 2547
MlyI	GAGTC	3 cut(s)	411, 1661, 1996
MmeI	TCCRAC	5 cut(s)	171, 1043, 1915, 1942, 2489
Mph1103I	ATGCAT	1 cut(s)	2446
MroI	TCCGGA	2 cut(s)	129, 2448
MroNI	GCCGGC	1 cut(s)	206
MroXI	GAANNNNTTC	1 cut(s)	509
MslI	CAYNNNNRTG	2 cut(s)	1694, 2053
MspA1I	CMGCKG	2 cut(s)	502, 2751
MspCI	CTTAAG	1 cut(s)	1520
MspR9I	CCNGG	5 cut(s)	203, 343, 576, 981, 1145
MunI	CAATTG	4 cut(s)	227, 2238, 2370, 2469
Mva1269I	GAATGC	3 cut(s)	1537, 2430, 2444
MvaI	CCWGG	3 cut(s)	203, 576, 1145
MvnI	CGCG	2 cut(s)	848, 1641
MwoI	GCNNNNNNNGC	9 cut(s)	99, 122, 207, 678, 1384, 1479, 2519, 2524, 2599
NaeI	GCCGGC	1 cut(s)	208
NciI	CCSGG	2 cut(s)	343, 981
NcoI	CCATGG	1 cut(s)	117
NgoMIV	GCCGGC	1 cut(s)	206
NmeAIII	GCCGAG	1 cut(s)	2761
NmuCI	GTSAC	2 cut(s)	1583, 1744
NsiI	ATGCAT	1 cut(s)	2446
NspI	RCATGY	1 cut(s)	1993
NspV	TTCGAA	2 cut(s)	281, 2156
PciSI	GCTCTTC	1 cut(s)	2359
PctI	GAATGC	3 cut(s)	1537, 2430, 2444
PdiI	GCCGGC	1 cut(s)	208
PdmI	GAANNNNTTC	1 cut(s)	509
PfeI	GAWTC	7 cut(s)	472, 543, 859, 1076, 1850, 2627, 2801
PinAI	ACCGGT	1 cut(s)	764
PkrI	GCNGC	8 cut(s)	48, 101, 258, 261, 291, 463, 2290, 2534
PleI	GAGTC	3 cut(s)	411, 1660, 1996
PpsI	GAGTC	3 cut(s)	411, 1660, 1996
Ppu21I	YACGTR	1 cut(s)	1104
PpuMI	RGGWCCY	1 cut(s)	361
PshAI	GACNNNNGTC	1 cut(s)	2565
Psp5II	RGGWCCY	1 cut(s)	361
Psp6I	CCWGG	3 cut(s)	201, 574, 1143
PspFI	CCCAGC	1 cut(s)	2522
PspGI	CCWGG	3 cut(s)	201, 574, 1143
PspPI	GGNCC	9 cut(s)	114, 162, 323, 361, 767, 778, 908, 1679, 2556
PspPPI	RGGWCCY	1 cut(s)	361
PsrI	GAACNNNNNNTAC	2 cut(s)	779, 811
PstNI	CAGNNNCTG	2 cut(s)	295, 2294
PsuI	RGATCY	1 cut(s)	2143
PvuII	CAGCTG	1 cut(s)	502
RsaI	GTAC	8 cut(s)	68, 78, 700, 1159, 1231, 1308, 1551, 1802
RsaNI	GTAC	8 cut(s)	67, 77, 699, 1158, 1230, 1307, 1550, 1801
RseI	CAYNNNNRTG	2 cut(s)	1694, 2053
SapI	GCTCTTC	1 cut(s)	2359
SatI	GCNGC	8 cut(s)	47, 100, 257, 260, 290, 462, 2289, 2533
Sau96I	GGNCC	9 cut(s)	114, 162, 323, 361, 767, 778, 908, 1679, 2556
SchI	GAGTC	3 cut(s)	411, 1661, 1996
ScrFI	CCNGG	5 cut(s)	203, 343, 576, 981, 1145
SduI	GDGCHC	2 cut(s)	683, 2547
SfaNI	GCATC	2 cut(s)	147, 2431
SfuI	TTCGAA	2 cut(s)	281, 2156
SgrAI	CRCCGGYG	1 cut(s)	833
SinI	GGWCC	7 cut(s)	162, 323, 361, 767, 778, 908, 1679
SmiMI	CAYNNNNRTG	2 cut(s)	1694, 2053
SmlI	CTYRAG	3 cut(s)	1520, 1995, 2309
SmoI	CTYRAG	3 cut(s)	1520, 1995, 2309
SspI	AATATT	1 cut(s)	1354
SspMI	CTAG	6 cut(s)	630, 1440, 2061, 2252, 2496, 2769
StyD4I	CCNGG	5 cut(s)	201, 341, 574, 979, 1143
StyI	CCWWGG	2 cut(s)	117, 2079
TaaI	ACNGT	8 cut(s)	1048, 1126, 1477, 1618, 1676, 1798, 2114, 2509
TaiI	ACGT	3 cut(s)	1106, 1332, 2322
TaqI	TCGA	8 cut(s)	281, 444, 638, 644, 785, 1724, 2156, 2655
TaqII	GACCGA	1 cut(s)	1009
TatI	WGTACW	3 cut(s)	76, 1549, 1800
TauI	GCSGC	2 cut(s)	102, 262
TfiI	GAWTC	7 cut(s)	472, 543, 859, 1076, 1850, 2627, 2801
TscAI	CASTG	4 cut(s)	976, 1129, 1681, 1842
TseFI	GTSAC	2 cut(s)	1583, 1744
TseI	GCWGC	6 cut(s)	46, 256, 289, 461, 2288, 2532
Tsp45I	GTSAC	2 cut(s)	1583, 1744
TspDTI	ATGAA	8 cut(s)	17, 1565, 1682, 1998, 2122, 2391, 2715, 2724
TspGWI	ACGGA	2 cut(s)	123, 1974
TspRI	CASTG	4 cut(s)	976, 1129, 1681, 1842
Vha464I	CTTAAG	1 cut(s)	1520
VpaK11BI	GGWCC	7 cut(s)	162, 323, 361, 767, 778, 908, 1679
XagI	CCTNNNNNAGG	1 cut(s)	2551
XapI	RAATTY	2 cut(s)	283, 509
XceI	RCATGY	1 cut(s)	1993
XcmI	CCANNNNNNNNNTGG	1 cut(s)	92
XmiI	GTMKAC	1 cut(s)	1516
XmnI	GAANNNNTTC	1 cut(s)	509
XspI	CTAG	6 cut(s)	630, 1440, 2061, 2252, 2496, 2769
Zsp2I	ATGCAT	1 cut(s)	2446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.