RchiOBHm_Chr4g0442231
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
Physical Location & Seq
Forward (+)
63908721 .. 63911253
2533 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41000

Sequence Viewer

Length: 1695 bp
ATGACCAGCTTGACTCCAGTTTGGCTACACGGTAATCAATTCACGGGTCCTTTACCAGAATTTTCAGAGCTTACCGACTTATTGGATTTGAATTTGGGGCATAACAAGCTCAAAGGTATTGTTCCTGCGTCTTTGCAAAACCTCAGTTTGCTTCAGAATGTCAGTTTGTCCAGCAATTTGCTTCAAGGACTTATGCCCAATTTCACCGGTGGTGCTATTAAGCAAGTGGACGTGACATCTAATAACTTTTGCACCAATGATGCTGGTACTAAATGCGATCCCCGCGTGACTATATTGCTCTCCATTGCCAAAGACATGGGTTACCCTACTGCGTTTGCAGAGAATTGGATAGGGAATGATCCTTGTGGTGGTAGTACTCATTGGACTGGTCTAACATGTTCAGGAAAAGATGACATTACAGTTCTCAATTTTCCCAACATGGGTCTGACGGGTACGATTTCTTCCAATTTTTCATTGCTTACATCCTTGAATAGATTGATACTTAGAGATAATAGTCTAACCGGTATTATACCCCAAGAGCTCACAAATTTGCACAACCTTGAAGAAATAGATCTCTCAAACAATAAACTTTGTGGTAAAGTACCTAGCTTTCGAGAAAACGTGGTGGTTAAGTTAGGAGGAAACCCAGACATAGGTAAGGACCATCCTAGTGGCCCTACTAATGGTGGAGGTAGCAAGAAATCCAAGACTGGTGTGATTGTTGGGGTTGTTATGGGATGTATTGTTGGAGCACTTCTAGTTGGTTTGCTTGCTTTTTATTTGCTTAAGAGAAAACATAGTCGTTATAGTAAAGTGCAAAGTCCAAATTCATTGGTTATACATCCTCGTCACTCTGGTGATCAAAATGCTGTCAAAATTACTGTTGCTAACAGGGTTAATGGCATTGGAAGTGAAAATCACAGTAACGGACCTAATGATATTCAAGTTGAGGCTGGAAATATGATAATTTCAATCCAGGTTTTGAGGGGTGTGACCAATAACTTCAATGAAGATAATATATTGGGAAAAGGTAGGTTTGGAACTGTGTATGAAGGAGAATTGCATGATGGAACAAAAATTGCAGTGAAGAGAATGGAATCCGGAGTGGTAGCTGAGAAAGGCCTGCATGAGTTCAAGTCTGAGATTGCAGTTCTTACTAAGGTCCGACATCGCCACTTGGTGGGACTTCTTGGATATTGTTTGGATGGGAATGAGAGGCTTCTTGTCTATGAAAGAAGGCTGGAGCCACTTGAATGGACTAGAAGACTAACCATTGCCTTGGATGTTGCGAGGGGTGTTCAATACCTGCATGGTTTGGCCAACGAAATCTTCATTCATAGAGACCTTAAACCTTCAAATATATTACTTGGAGATGATATGCGGGCGAAAGTGGCAGATTTTGGATTGGTTTGTCTTGCTCCAGCAGGGAAAGCCTCTATTGAGACAAGGCTAGCAGGAACTTTTGGCTATTTGGCTCCAGAATATGCAGATGTTTTCAGCTTCGGAGTAATATTAATGGAGTTGATCACGGGAAGAAAAGCACTTGACGAAAGCCAGCCTGAGGAGAGTTGTCACCTAGTGACATGGTTTCATAGAAAGATCATTAAAACAGATGCACTCCCAGAGATGGTTGATCCAAGAATTAATGTCAATGAGGAAACAATTTCTAGTATTAGTACAGTTGCTGAGTGTTGA

Protein Analysis

564

Amino Acids

61.34

Weight (kDa)

6.5

Isoelectric Point (pI)

27.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 21 - 61 9e-06 Leucine rich repeat
LRR_8 PF13855 150 - 197 7.6e-06 Leucine rich repeat
Pkinase PF00069 336 - 537 1.1e-30 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 338 - 528 2.6e-28 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1314
AccB7I CCANNNNNTGG 1 cut(s) 1180
AccII CGCG 1 cut(s) 285
AccIII TCCGGA 1 cut(s) 1100
AciI CCGC 2 cut(s) 283, 1381
AclWI GGATC 3 cut(s) 272, 353, 1628
AcoI YGGCCR 1 cut(s) 1317
AcsI RAATTY 4 cut(s) 59, 91, 547, 826
AcuI CTGAAG 1 cut(s) 137
AdeI CACNNNGTG 2 cut(s) 1180, 1579
AfaI GTAC 5 cut(s) 268, 376, 454, 603, 1678
AfiI CCNNNNNNNGG 7 cut(s) 368, 440, 653, 683, 1180, 1561, 1627
AflII CTTAAG 1 cut(s) 785
AflIII ACRYGT 1 cut(s) 395
AgeI ACCGGT 2 cut(s) 206, 521
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 1 cut(s) 975
AjuI GAANNNNNNNTTGG 2 cut(s) 1313, 1345
AleI CACNNNNGTG 1 cut(s) 855
AluBI AGCT 7 cut(s) 9, 70, 109, 541, 609, 1112, 1500
AluI AGCT 7 cut(s) 9, 70, 109, 541, 609, 1112, 1500
Alw21I GWGCWC 2 cut(s) 543, 754
Alw26I GTCTC 2 cut(s) 1335, 1436
AlwI GGATC 3 cut(s) 272, 353, 1628
AlwNI CAGNNNCTG 1 cut(s) 1685
Aor13HI TCCGGA 1 cut(s) 1100
AoxI GGCC 3 cut(s) 673, 1120, 1317
ApoI RAATTY 4 cut(s) 59, 91, 547, 826
AseI ATTAAT 2 cut(s) 1514, 1644
AsiGI ACCGGT 2 cut(s) 206, 521
AspS9I GGNCC 5 cut(s) 47, 661, 674, 929, 1162
AsuHPI GGTGA 3 cut(s) 196, 869, 1565
AsuNHI GCTAGC 1 cut(s) 1450
AvaII GGWCC 4 cut(s) 47, 661, 929, 1162
AxyI CCTNAGG 1 cut(s) 1560
BalI TGGCCA 1 cut(s) 1319
BanII GRGCYC 1 cut(s) 543
BarI GAAGNNNNNNTAC 2 cut(s) 445, 477
BbsI GAAGAC 1 cut(s) 1270
Bbv12I GWGCWC 2 cut(s) 543, 754
BccI CCATC 4 cut(s) 672, 1061, 1199, 1621
BciT130I CCWGG 1 cut(s) 977
BclI TGATCA 2 cut(s) 859, 1524
BcoDI GTCTC 2 cut(s) 1335, 1436
BfaI CTAG 7 cut(s) 606, 669, 758, 1260, 1451, 1577, 1668
BfrI CTTAAG 1 cut(s) 785
BfuAI ACCTGC 1 cut(s) 1314
BglII AGATCT 1 cut(s) 571
BmcAI AGTACT 1 cut(s) 376
Bme1390I CCNGG 1 cut(s) 977
Bme18I GGWCC 4 cut(s) 47, 661, 929, 1162
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 5 cut(s) 47, 661, 674, 929, 1162
BmiI GGNNCC 3 cut(s) 48, 1245, 1476
BmrFI CCNGG 1 cut(s) 977
BmsI GCATC 2 cut(s) 250, 1603
BmtI GCTAGC 1 cut(s) 1454
BpiI GAAGAC 1 cut(s) 1270
BpmI CTGGAG 3 cut(s) 1262, 1404, 1461
BsaI GGTCTC 1 cut(s) 1335
BsaJI CCNNGG 1 cut(s) 1278
BsaWI WCCGGW 3 cut(s) 206, 521, 1100
Bsc4I CCNNNNNNNGG 7 cut(s) 368, 440, 653, 683, 1180, 1561, 1627
Bse118I RCCGGY 2 cut(s) 206, 521
Bse1I ACTGG 3 cut(s) 17, 391, 715
Bse21I CCTNAGG 1 cut(s) 1560
Bse3DI GCAATG 3 cut(s) 303, 473, 1272
BseAI TCCGGA 1 cut(s) 1100
BseBI CCWGG 1 cut(s) 977
BseDI CCNNGG 1 cut(s) 1278
BseGI GGATG 6 cut(s) 482, 664, 743, 841, 1210, 1288
BseLI CCNNNNNNNGG 7 cut(s) 368, 440, 653, 683, 1180, 1561, 1627
BseMI GCAATG 3 cut(s) 303, 473, 1272
BseMII CTCAG 5 cut(s) 157, 1104, 1131, 1551, 1677
BseNI ACTGG 3 cut(s) 17, 391, 715
BseRI GAGGAG 1 cut(s) 1577
Bsh1236I CGCG 1 cut(s) 285
BshFI GGCC 3 cut(s) 675, 1122, 1319
BshTI ACCGGT 2 cut(s) 206, 521
BsiHKAI GWGCWC 2 cut(s) 543, 754
BsiSI CCGG 3 cut(s) 207, 522, 1101
BslFI GGGAC 1 cut(s) 1197
BslI CCNNNNNNNGG 7 cut(s) 368, 440, 653, 683, 1180, 1561, 1627
BsmAI GTCTC 2 cut(s) 1335, 1436
BsmFI GGGAC 1 cut(s) 1197
BsnI GGCC 3 cut(s) 675, 1122, 1319
Bso31I GGTCTC 1 cut(s) 1335
Bsp1286I GDGCHC 2 cut(s) 543, 754
Bsp13I TCCGGA 1 cut(s) 1100
Bsp143I GATC 7 cut(s) 277, 358, 571, 859, 1524, 1599, 1633
BspACI CCGC 2 cut(s) 283, 1381
BspANI GGCC 3 cut(s) 675, 1122, 1319
BspCNI CTCAG 5 cut(s) 156, 1105, 1132, 1552, 1678
BspEI TCCGGA 1 cut(s) 1100
BspFNI CGCG 1 cut(s) 285
BspLI GGNNCC 3 cut(s) 48, 1245, 1476
BspMI ACCTGC 1 cut(s) 1314
BspOI GCTAGC 1 cut(s) 1454
BspPI GGATC 3 cut(s) 272, 353, 1628
BspTI CTTAAG 1 cut(s) 785
BspTNI GGTCTC 1 cut(s) 1335
BsrDI GCAATG 3 cut(s) 303, 473, 1272
BsrFI RCCGGY 2 cut(s) 206, 521
BsrI ACTGG 3 cut(s) 17, 391, 715
BssAI RCCGGY 2 cut(s) 206, 521
BssECI CCNNGG 1 cut(s) 1278
BssMI GATC 7 cut(s) 277, 358, 571, 859, 1524, 1599, 1633
BssT1I CCWWGG 1 cut(s) 1278
Bst2UI CCWGG 1 cut(s) 977
Bst4CI ACNGT 6 cut(s) 32, 421, 883, 923, 1045, 1681
Bst6I CTCTTC 1 cut(s) 1082
BstAFI CTTAAG 1 cut(s) 785
BstC8I GCNNGC 5 cut(s) 771, 1124, 1383, 1452, 1556
BstDEI CTNAG 7 cut(s) 143, 503, 1113, 1140, 1158, 1560, 1686
BstEII GGTNACC 1 cut(s) 320
BstF5I GGATG 6 cut(s) 482, 664, 743, 841, 1210, 1288
BstFNI CGCG 1 cut(s) 285
BstKTI GATC 7 cut(s) 280, 361, 574, 862, 1527, 1602, 1636
BstMAI GTCTC 2 cut(s) 1335, 1436
BstMBI GATC 7 cut(s) 277, 358, 571, 859, 1524, 1599, 1633
BstMWI GCNNNNNNNGC 4 cut(s) 106, 282, 1391, 1430
BstNI CCWGG 1 cut(s) 977
BstNSI RCATGY 1 cut(s) 399
BstPI GGTNACC 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 975
BstUI CGCG 1 cut(s) 285
BstV2I GAAGAC 1 cut(s) 1270
BstX2I RGATCY 1 cut(s) 571
BstXI CCANNNNNNTGG 4 cut(s) 316, 671, 1254, 1279
BstYI RGATCY 1 cut(s) 571
Bsu36I CCTNAGG 1 cut(s) 1560
BsuRI GGCC 3 cut(s) 675, 1122, 1319
BtgZI GCGATG 1 cut(s) 1154
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 6 cut(s) 482, 664, 743, 841, 1210, 1288
BtsI GCAGTG 1 cut(s) 1089
BtsIMutI CAGTG 1 cut(s) 1089
BveI ACCTGC 1 cut(s) 1314
Cac8I GCNNGC 5 cut(s) 771, 1124, 1383, 1452, 1556
CaiI CAGNNNCTG 1 cut(s) 1685
Cfr10I RCCGGY 2 cut(s) 206, 521
Cfr13I GGNCC 5 cut(s) 47, 661, 674, 929, 1162
CseI GACGC 1 cut(s) 117
Csp6I GTAC 5 cut(s) 267, 375, 453, 602, 1677
CspAI ACCGGT 2 cut(s) 206, 521
CviAII CATG 7 cut(s) 316, 396, 439, 1064, 1127, 1310, 1584
CviQI GTAC 5 cut(s) 267, 375, 453, 602, 1677
DdeI CTNAG 7 cut(s) 143, 503, 1113, 1140, 1158, 1560, 1686
DpnI GATC 7 cut(s) 279, 360, 573, 861, 1526, 1601, 1635
DpnII GATC 7 cut(s) 277, 358, 571, 859, 1524, 1599, 1633
DraIII CACNNNGTG 2 cut(s) 1180, 1579
EaeI YGGCCR 1 cut(s) 1317
Eam1104I CTCTTC 1 cut(s) 1082
EarI CTCTTC 1 cut(s) 1082
Ecl136II GAGCTC 1 cut(s) 541
Eco130I CCWWGG 1 cut(s) 1278
Eco147I AGGCCT 1 cut(s) 1122
Eco24I GRGCYC 1 cut(s) 543
Eco31I GGTCTC 1 cut(s) 1335
Eco47I GGWCC 4 cut(s) 47, 661, 929, 1162
Eco53kI GAGCTC 1 cut(s) 541
Eco57I CTGAAG 1 cut(s) 137
Eco81I CCTNAGG 1 cut(s) 1560
Eco91I GGTNACC 1 cut(s) 320
EcoICRI GAGCTC 1 cut(s) 541
EcoO109I RGGNCCY 1 cut(s) 47
EcoO65I GGTNACC 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 975
EcoT14I CCWWGG 1 cut(s) 1278
EcoT38I GRGCYC 1 cut(s) 543
ErhI CCWWGG 1 cut(s) 1278
FaeI CATG 7 cut(s) 319, 399, 442, 1067, 1130, 1313, 1587
FaqI GGGAC 1 cut(s) 1197
FatI CATG 7 cut(s) 315, 395, 438, 1063, 1126, 1309, 1583
FauI CCCGC 2 cut(s) 290, 1374
FbaI TGATCA 2 cut(s) 859, 1524
FokI GGATG 6 cut(s) 469, 651, 750, 828, 1217, 1295
FriOI GRGCYC 1 cut(s) 543
FspBI CTAG 7 cut(s) 606, 669, 758, 1260, 1451, 1577, 1668
GsuI CTGGAG 3 cut(s) 1262, 1404, 1461
HaeIII GGCC 3 cut(s) 675, 1122, 1319
HapII CCGG 3 cut(s) 207, 522, 1101
HgaI GACGC 1 cut(s) 117
Hin1II CATG 7 cut(s) 319, 399, 442, 1067, 1130, 1313, 1587
HinfI GANTC 2 cut(s) 13, 1097
HpaII CCGG 3 cut(s) 207, 522, 1101
HphI GGTGA 3 cut(s) 196, 869, 1565
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 6 cut(s) 67, 156, 447, 1141, 1166, 1505
Hpy188III TCNNGA 4 cut(s) 402, 614, 1101, 1478
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 3 cut(s) 1046, 1230, 1362
HpyCH4III ACNGT 6 cut(s) 32, 421, 883, 923, 1045, 1681
HpyCH4IV ACGT 2 cut(s) 231, 621
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 282, 1391, 1430
HpyF3I CTNAG 7 cut(s) 143, 503, 1113, 1140, 1158, 1560, 1686
HpySE526I ACGT 2 cut(s) 231, 621
Hsp92II CATG 7 cut(s) 319, 399, 442, 1067, 1130, 1313, 1587
Kpn2I TCCGGA 1 cut(s) 1100
Ksp22I TGATCA 2 cut(s) 859, 1524
Kzo9I GATC 7 cut(s) 277, 358, 571, 859, 1524, 1599, 1633
LmnI GCTCC 4 cut(s) 749, 1243, 1423, 1480
LweI GCATC 2 cut(s) 250, 1603
MaeI CTAG 7 cut(s) 606, 669, 758, 1260, 1451, 1577, 1668
MaeII ACGT 2 cut(s) 231, 621
MaeIII GTNAC 8 cut(s) 232, 286, 320, 848, 923, 991, 1571, 1579
MalI GATC 7 cut(s) 279, 360, 573, 861, 1526, 1601, 1635
MboI GATC 7 cut(s) 277, 358, 571, 859, 1524, 1599, 1633
MboII GAAGA 7 cut(s) 453, 575, 1022, 1099, 1275, 1321, 1545
MflI RGATCY 1 cut(s) 571
MhlI GDGCHC 2 cut(s) 543, 754
MlsI TGGCCA 1 cut(s) 1319
MluNI TGGCCA 1 cut(s) 1319
MlyI GAGTC 1 cut(s) 7
MmeI TCCRAC 2 cut(s) 727, 1189
Mox20I TGGCCA 1 cut(s) 1319
MroI TCCGGA 1 cut(s) 1100
MscI TGGCCA 1 cut(s) 1319
MseI TTAA 8 cut(s) 219, 630, 786, 897, 1347, 1514, 1605, 1644
MslI CAYNNNNRTG 3 cut(s) 669, 855, 1252
Msp20I TGGCCA 1 cut(s) 1319
MspCI CTTAAG 1 cut(s) 785
MspI CCGG 3 cut(s) 207, 522, 1101
MspR9I CCNGG 1 cut(s) 977
MvaI CCWGG 1 cut(s) 977
MvnI CGCG 1 cut(s) 285
MwoI GCNNNNNNNGC 4 cut(s) 106, 282, 1391, 1430
NdeII GATC 7 cut(s) 277, 358, 571, 859, 1524, 1599, 1633
NheI GCTAGC 1 cut(s) 1450
NlaIII CATG 7 cut(s) 319, 399, 442, 1067, 1130, 1313, 1587
NlaIV GGNNCC 3 cut(s) 48, 1245, 1476
NmuCI GTSAC 6 cut(s) 232, 286, 848, 991, 1571, 1579
NspI RCATGY 1 cut(s) 399
OliI CACNNNNGTG 1 cut(s) 855
PceI AGGCCT 1 cut(s) 1122
PciI ACATGT 1 cut(s) 395
PfeI GAWTC 1 cut(s) 1097
PflMI CCANNNNNTGG 1 cut(s) 1180
PinAI ACCGGT 2 cut(s) 206, 521
PleI GAGTC 1 cut(s) 7
PpsI GAGTC 1 cut(s) 7
PpuMI RGGWCCY 1 cut(s) 47
PscI ACATGT 1 cut(s) 395
PshBI ATTAAT 2 cut(s) 1514, 1644
Psp124BI GAGCTC 1 cut(s) 543
Psp5II RGGWCCY 1 cut(s) 47
Psp6I CCWGG 1 cut(s) 975
PspEI GGTNACC 1 cut(s) 320
PspGI CCWGG 1 cut(s) 975
PspN4I GGNNCC 3 cut(s) 48, 1245, 1476
PspPI GGNCC 5 cut(s) 47, 661, 674, 929, 1162
PspPPI RGGWCCY 1 cut(s) 47
PstNI CAGNNNCTG 1 cut(s) 1685
PsuI RGATCY 1 cut(s) 571
RsaI GTAC 5 cut(s) 268, 376, 454, 603, 1678
RsaNI GTAC 5 cut(s) 267, 375, 453, 602, 1677
RseI CAYNNNNRTG 3 cut(s) 669, 855, 1252
SacI GAGCTC 1 cut(s) 543
SaqAI TTAA 8 cut(s) 219, 630, 786, 897, 1347, 1514, 1605, 1644
Sau3AI GATC 7 cut(s) 277, 358, 571, 859, 1524, 1599, 1633
Sau96I GGNCC 5 cut(s) 47, 661, 674, 929, 1162
ScaI AGTACT 1 cut(s) 376
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 1 cut(s) 977
SduI GDGCHC 2 cut(s) 543, 754
SfaNI GCATC 2 cut(s) 250, 1603
SgrAI CRCCGGYG 1 cut(s) 206
SinI GGWCC 4 cut(s) 47, 661, 929, 1162
SmiMI CAYNNNNRTG 3 cut(s) 669, 855, 1252
SmlI CTYRAG 1 cut(s) 785
SmoI CTYRAG 1 cut(s) 785
SseBI AGGCCT 1 cut(s) 1122
SsiI CCGC 2 cut(s) 283, 1381
SspI AATATT 1 cut(s) 1512
SspMI CTAG 7 cut(s) 606, 669, 758, 1260, 1451, 1577, 1668
SstI GAGCTC 1 cut(s) 543
StuI AGGCCT 1 cut(s) 1122
StyD4I CCNGG 1 cut(s) 975
StyI CCWWGG 1 cut(s) 1278
TaaI ACNGT 6 cut(s) 32, 421, 883, 923, 1045, 1681
TaiI ACGT 2 cut(s) 234, 624
TaqI TCGA 1 cut(s) 613
TatI WGTACW 2 cut(s) 374, 1676
TfiI GAWTC 1 cut(s) 1097
Tru1I TTAA 8 cut(s) 219, 630, 786, 897, 1347, 1514, 1605, 1644
Tru9I TTAA 8 cut(s) 219, 630, 786, 897, 1347, 1514, 1605, 1644
TscAI CASTG 1 cut(s) 1089
TseFI GTSAC 6 cut(s) 232, 286, 848, 991, 1571, 1579
Tsp45I GTSAC 6 cut(s) 232, 286, 848, 991, 1571, 1579
TspDTI ATGAA 8 cut(s) 462, 819, 1023, 1065, 1245, 1321, 1325, 1580
TspGWI ACGGA 1 cut(s) 942
TspRI CASTG 1 cut(s) 1089
Van91I CCANNNNNTGG 1 cut(s) 1180
Vha464I CTTAAG 1 cut(s) 785
VpaK11BI GGWCC 4 cut(s) 47, 661, 929, 1162
VspI ATTAAT 2 cut(s) 1514, 1644
XapI RAATTY 4 cut(s) 59, 91, 547, 826
XceI RCATGY 1 cut(s) 399
XspI CTAG 7 cut(s) 606, 669, 758, 1260, 1451, 1577, 1668
ZrmI AGTACT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.