Rh4CG420200
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0003113
Physical Location & Seq
Forward (+)
66436855 .. 66438044
1190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG420200.1

Sequence Viewer

Length: 1047 bp
ATGGGTTTCTGGGGTTGTGTCTTCGTTTTGCTGTCACTGTCCGCACCTGTGCATTCCCAGTCCCAAAAGCTCAGCGAGGACCAGCCAGCCATGGAAGCTATTCTCGAAAACATCAAACCAAAACCACCCACTAATTGGTCGTCTGGCTCCGATTGCTGTACCTGGGGCGACGTAACTTGCGAAAATGGCAAGGTGGTCACCATCATGATCAAAGATCAACATCTCTCCGGCTCTCTCCATCCAGACATCAATAAGCTGACTTCTTTGCAGCGTCTGCACATTATGCGCAACAGTCTCACCGGACCCTTCCCAACCCTACAAGGCTTGGCAGAGCTTGAGGAGATTTATGCCAACCAGAACAACTTCACTTCTTTTCCAGTAGATGATTTCTTCTCCGGCCTCGATTCCCTTCAAGAATTCCACGTCGACTACAACCCCTTCCAGGCGTGGCAGATTCCTGATAGCACTAAGTTTGCCTCCAAGCTGACCAGTTTCACAGCTAGTGATGCAAACATTTCAGGAAAGATCCCGGAATTTTTTGACAGCTACAATATGCCTAGTTTGGTTACCTTGGAGTTGGCTTATAATAATCTCGAAGGTGAATTGCCTGCCAGTTTTTCCGGTTCCGGGATTCAGTCCCTTTTGTTGAACAACAACAATAAGCTCAATGGGACTATCTCTGTGTTACAGAACATGACCAGCTTGACTCGAGTTTGGCTACATGGTAATCAATTCACGGGTCATTTGCCAGAATTTTCAGAGCTTACCAACTTATTGGATTTGAATTTGGGGCATAACAAGCTCACAGGTATTGTTCCTGCGTCTTTGCAAAACCTCAGTTTGCTTCAGAATGTCAGTTTGTCCAGCAATTTGCTTCAAGAACCTATGCCCAATTTCACCGGTGGTGCTATTAAGCAAGTGGACGTGACATCTAATAACTTTTGCACCAACGATGCTGGTACTAAATGCGATCCCCGCGTGACTATATTGCTCTCCATTGCCAAAGACATGGGTTACTGCGTTTGCAGAGAATTGGATAGGGAATGA

Protein Analysis

348

Amino Acids

38.31

Weight (kDa)

4.86

Isoelectric Point (pI)

35.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 234 - 292 5.4e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
Acc16I TGCGCA 1 cut(s) 287
AccB7I CCANNNNNTGG 1 cut(s) 135
AccI GTMKAC 1 cut(s) 426
AccII CGCG 1 cut(s) 978
AciI CCGC 2 cut(s) 42, 976
AclWI GGATC 2 cut(s) 520, 965
AcsI RAATTY 4 cut(s) 416, 533, 752, 784
AcuI CTGAAG 1 cut(s) 830
AfaI GTAC 2 cut(s) 160, 961
AfiI CCNNNNNNNGG 3 cut(s) 135, 442, 627
AgeI ACCGGT 1 cut(s) 899
AgsI TTSAA 4 cut(s) 413, 649, 784, 878
AjiI CACGTC 2 cut(s) 424, 925
AjnI CCWGG 2 cut(s) 161, 441
Alw26I GTCTC 1 cut(s) 299
AlwI GGATC 2 cut(s) 520, 965
AlwNI CAGNNNCTG 1 cut(s) 274
Ama87I CYCGRG 1 cut(s) 708
AoxI GGCC 1 cut(s) 397
ApeKI GCWGC 1 cut(s) 268
ApoI RAATTY 4 cut(s) 416, 533, 752, 784
AsiGI ACCGGT 1 cut(s) 899
Asp700I GAANNNNTTC 2 cut(s) 99, 362
AspLEI GCGC 1 cut(s) 288
AspS9I GGNCC 2 cut(s) 79, 302
AsuC2I CCSGG 2 cut(s) 530, 628
AsuHPI GGTGA 4 cut(s) 190, 289, 611, 889
AvaI CYCGRG 1 cut(s) 708
AvaII GGWCC 2 cut(s) 79, 302
BbsI GAAGAC 1 cut(s) 13
BbvI GCAGC 1 cut(s) 280
BccI CCATC 2 cut(s) 209, 246
BciT130I CCWGG 2 cut(s) 163, 443
BclI TGATCA 1 cut(s) 207
BcnI CCSGG 2 cut(s) 530, 628
BcoDI GTCTC 1 cut(s) 299
BfaI CTAG 2 cut(s) 501, 558
BisI GCNGC 1 cut(s) 269
BlpI GCTNAGC 1 cut(s) 71
BlsI GCNGC 1 cut(s) 270
Bme1390I CCNGG 4 cut(s) 163, 443, 530, 628
Bme18I GGWCC 2 cut(s) 79, 302
BmeT110I CYCGRG 1 cut(s) 708
BmgBI CACGTC 2 cut(s) 424, 925
BmgT120I GGNCC 2 cut(s) 79, 302
BmiI GGNNCC 3 cut(s) 148, 304, 625
BmrFI CCNGG 4 cut(s) 163, 443, 530, 628
BmrI ACTGGG 1 cut(s) 52
BmsI GCATC 2 cut(s) 496, 943
BmuI ACTGGG 1 cut(s) 52
BpiI GAAGAC 1 cut(s) 13
Bpu1102I GCTNAGC 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 2 cut(s) 530, 628
BsaBI GATNNNNATC 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 90, 162, 570
BsaWI WCCGGW 3 cut(s) 299, 620, 899
Bsc4I CCNNNNNNNGG 3 cut(s) 135, 442, 627
Bse118I RCCGGY 1 cut(s) 899
Bse1I ACTGG 4 cut(s) 58, 377, 489, 612
Bse3DI GCAATG 1 cut(s) 996
Bse8I GATNNNNATC 1 cut(s) 219
BseBI CCWGG 2 cut(s) 163, 443
BseDI CCNNGG 3 cut(s) 90, 162, 570
BseGI GGATG 1 cut(s) 238
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 3 cut(s) 135, 442, 627
BseMI GCAATG 1 cut(s) 996
BseMII CTCAG 2 cut(s) 85, 850
BseNI ACTGG 4 cut(s) 58, 377, 489, 612
BseRI GAGGAG 1 cut(s) 353
BseXI GCAGC 1 cut(s) 280
BsgI GTGCAG 1 cut(s) 260
Bsh1236I CGCG 1 cut(s) 978
BshFI GGCC 1 cut(s) 399
BshTI ACCGGT 1 cut(s) 899
BsiHKCI CYCGRG 1 cut(s) 708
BsiSI CCGG 7 cut(s) 228, 300, 396, 530, 621, 627, 900
BslFI GGGAC 3 cut(s) 46, 622, 685
BslI CCNNNNNNNGG 3 cut(s) 135, 442, 627
BsmAI GTCTC 1 cut(s) 299
BsmFI GGGAC 3 cut(s) 46, 622, 685
BsmI GAATGC 1 cut(s) 52
BsnI GGCC 1 cut(s) 399
BsoBI CYCGRG 1 cut(s) 708
Bsp143I GATC 4 cut(s) 207, 214, 525, 970
Bsp1720I GCTNAGC 1 cut(s) 71
Bsp19I CCATGG 1 cut(s) 90
BspACI CCGC 2 cut(s) 42, 976
BspANI GGCC 1 cut(s) 399
BspCNI CTCAG 2 cut(s) 84, 849
BspFNI CGCG 1 cut(s) 978
BspHI TCATGA 1 cut(s) 204
BspLI GGNNCC 3 cut(s) 148, 304, 625
BspPI GGATC 2 cut(s) 520, 965
BsrDI GCAATG 1 cut(s) 996
BsrFI RCCGGY 1 cut(s) 899
BsrI ACTGG 4 cut(s) 58, 377, 489, 612
BssAI RCCGGY 1 cut(s) 899
BssECI CCNNGG 3 cut(s) 90, 162, 570
BssMI GATC 4 cut(s) 207, 214, 525, 970
BssT1I CCWWGG 2 cut(s) 90, 570
Bst2UI CCWGG 2 cut(s) 163, 443
Bst4CI ACNGT 2 cut(s) 39, 293
BstAPI GCANNNNNTGC 2 cut(s) 274, 283
BstC8I GCNNGC 2 cut(s) 87, 609
BstDEI CTNAG 3 cut(s) 71, 468, 836
BstDSI CCRYGG 1 cut(s) 90
BstEII GGTNACC 2 cut(s) 196, 565
BstF5I GGATG 1 cut(s) 238
BstFNI CGCG 1 cut(s) 978
BstHHI GCGC 1 cut(s) 288
BstKTI GATC 4 cut(s) 210, 217, 528, 973
BstMAI GTCTC 1 cut(s) 299
BstMBI GATC 4 cut(s) 207, 214, 525, 970
BstMWI GCNNNNNNNGC 8 cut(s) 95, 153, 186, 274, 283, 506, 799, 975
BstNI CCWGG 2 cut(s) 163, 443
BstPI GGTNACC 2 cut(s) 196, 565
BstSCI CCNGG 4 cut(s) 161, 441, 528, 626
BstUI CGCG 1 cut(s) 978
BstV1I GCAGC 1 cut(s) 280
BstV2I GAAGAC 1 cut(s) 13
BstX2I RGATCY 1 cut(s) 525
BstXI CCANNNNNNTGG 2 cut(s) 775, 1009
BstYI RGATCY 1 cut(s) 525
BsuRI GGCC 1 cut(s) 399
BtgI CCRYGG 1 cut(s) 90
BtrI CACGTC 2 cut(s) 424, 925
BtsCI GGATG 1 cut(s) 238
BtsIMutI CAGTG 1 cut(s) 35
Cac8I GCNNGC 2 cut(s) 87, 609
CaiI CAGNNNCTG 1 cut(s) 274
CciI TCATGA 1 cut(s) 204
CfoI GCGC 1 cut(s) 288
Cfr10I RCCGGY 1 cut(s) 899
Cfr13I GGNCC 2 cut(s) 79, 302
CseI GACGC 2 cut(s) 260, 810
Csp6I GTAC 2 cut(s) 159, 960
CspAI ACCGGT 1 cut(s) 899
CviAII CATG 5 cut(s) 91, 205, 694, 722, 1009
CviQI GTAC 2 cut(s) 159, 960
DdeI CTNAG 3 cut(s) 71, 468, 836
DpnI GATC 4 cut(s) 209, 216, 527, 972
DpnII GATC 4 cut(s) 207, 214, 525, 970
Eco130I CCWWGG 2 cut(s) 90, 570
Eco47I GGWCC 2 cut(s) 79, 302
Eco57I CTGAAG 1 cut(s) 830
Eco88I CYCGRG 1 cut(s) 708
Eco91I GGTNACC 2 cut(s) 196, 565
EcoO65I GGTNACC 2 cut(s) 196, 565
EcoRI GAATTC 1 cut(s) 416
EcoRII CCWGG 2 cut(s) 161, 441
EcoT14I CCWWGG 2 cut(s) 90, 570
ErhI CCWWGG 2 cut(s) 90, 570
FaeI CATG 5 cut(s) 94, 208, 697, 725, 1012
FaqI GGGAC 3 cut(s) 46, 622, 685
FatI CATG 5 cut(s) 90, 204, 693, 721, 1008
FauI CCCGC 1 cut(s) 983
FbaI TGATCA 1 cut(s) 207
FblI GTMKAC 1 cut(s) 426
Fnu4HI GCNGC 1 cut(s) 269
FokI GGATG 1 cut(s) 225
Fsp4HI GCNGC 1 cut(s) 269
FspBI CTAG 2 cut(s) 501, 558
FspI TGCGCA 1 cut(s) 287
GlaI GCGC 1 cut(s) 287
GluI GCNGC 1 cut(s) 269
HaeIII GGCC 1 cut(s) 399
HapII CCGG 7 cut(s) 228, 300, 396, 530, 621, 627, 900
HgaI GACGC 2 cut(s) 260, 810
HhaI GCGC 1 cut(s) 288
Hin1II CATG 5 cut(s) 94, 208, 697, 725, 1012
Hin6I GCGC 1 cut(s) 286
HinP1I GCGC 1 cut(s) 286
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HinfI GANTC 4 cut(s) 404, 454, 631, 706
HpaII CCGG 7 cut(s) 228, 300, 396, 530, 621, 627, 900
HphI GGTGA 4 cut(s) 190, 289, 611, 889
Hpy166II GTNNAC 2 cut(s) 427, 922
Hpy188I TCNGA 3 cut(s) 151, 760, 849
Hpy188III TCNNGA 8 cut(s) 104, 205, 242, 413, 458, 519, 593, 878
Hpy8I GTNNAC 2 cut(s) 427, 922
Hpy99I CGWCG 2 cut(s) 173, 428
HpyAV CCTTC 4 cut(s) 316, 419, 448, 590
HpyCH4III ACNGT 2 cut(s) 39, 293
HpyCH4IV ACGT 3 cut(s) 171, 423, 924
HpyCH4V TGCA 7 cut(s) 52, 268, 277, 509, 829, 945, 1026
HpyF10VI GCNNNNNNNGC 8 cut(s) 95, 153, 186, 274, 283, 506, 799, 975
HpyF3I CTNAG 3 cut(s) 71, 468, 836
HpySE526I ACGT 3 cut(s) 171, 423, 924
Hsp92II CATG 5 cut(s) 94, 208, 697, 725, 1012
HspAI GCGC 1 cut(s) 286
Ksp22I TGATCA 1 cut(s) 207
Kzo9I GATC 4 cut(s) 207, 214, 525, 970
LmnI GCTCC 1 cut(s) 152
Lsp1109I GCAGC 1 cut(s) 280
LweI GCATC 2 cut(s) 496, 943
MaeI CTAG 2 cut(s) 501, 558
MaeII ACGT 3 cut(s) 171, 423, 924
MaeIII GTNAC 8 cut(s) 33, 172, 196, 565, 684, 925, 979, 1013
MalI GATC 4 cut(s) 209, 216, 527, 972
MboI GATC 4 cut(s) 207, 214, 525, 970
MboII GAAGA 2 cut(s) 13, 382
MflI RGATCY 1 cut(s) 525
MlyI GAGTC 1 cut(s) 700
MnlI CCTC 5 cut(s) 70, 331, 410, 487, 845
MroXI GAANNNNTTC 2 cut(s) 99, 362
MseI TTAA 1 cut(s) 912
MslI CAYNNNNRTG 1 cut(s) 203
MspI CCGG 7 cut(s) 228, 300, 396, 530, 621, 627, 900
MspR9I CCNGG 4 cut(s) 163, 443, 530, 628
Mva1269I GAATGC 1 cut(s) 52
MvaI CCWGG 2 cut(s) 163, 443
MvnI CGCG 1 cut(s) 978
MwoI GCNNNNNNNGC 8 cut(s) 95, 153, 186, 274, 283, 506, 799, 975
NciI CCSGG 2 cut(s) 530, 628
NcoI CCATGG 1 cut(s) 90
NdeII GATC 4 cut(s) 207, 214, 525, 970
NlaIII CATG 5 cut(s) 94, 208, 697, 725, 1012
NlaIV GGNNCC 3 cut(s) 148, 304, 625
NmuCI GTSAC 4 cut(s) 33, 196, 925, 979
NsbI TGCGCA 1 cut(s) 287
PaeR7I CTCGAG 1 cut(s) 708
PagI TCATGA 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PctI GAATGC 1 cut(s) 52
PdmI GAANNNNTTC 2 cut(s) 99, 362
PfeI GAWTC 3 cut(s) 404, 454, 631
PflMI CCANNNNNTGG 1 cut(s) 135
PfoI TCCNGGA 2 cut(s) 528, 626
PinAI ACCGGT 1 cut(s) 899
PkrI GCNGC 1 cut(s) 270
PleI GAGTC 1 cut(s) 700
PpsI GAGTC 1 cut(s) 700
PsiI TTATAA 1 cut(s) 585
Psp6I CCWGG 2 cut(s) 161, 441
PspEI GGTNACC 2 cut(s) 196, 565
PspGI CCWGG 2 cut(s) 161, 441
PspN4I GGNNCC 3 cut(s) 148, 304, 625
PspPI GGNCC 2 cut(s) 79, 302
PspXI VCTCGAGB 1 cut(s) 708
PstNI CAGNNNCTG 1 cut(s) 274
PsuI RGATCY 1 cut(s) 525
RsaI GTAC 2 cut(s) 160, 961
RsaNI GTAC 2 cut(s) 159, 960
RseI CAYNNNNRTG 1 cut(s) 203
SalI GTCGAC 1 cut(s) 425
SaqAI TTAA 1 cut(s) 912
SatI GCNGC 1 cut(s) 269
Sau3AI GATC 4 cut(s) 207, 214, 525, 970
Sau96I GGNCC 2 cut(s) 79, 302
SchI GAGTC 1 cut(s) 700
ScrFI CCNGG 4 cut(s) 163, 443, 530, 628
SfaNI GCATC 2 cut(s) 496, 943
Sfr274I CTCGAG 1 cut(s) 708
SgrAI CRCCGGYG 1 cut(s) 899
SinI GGWCC 2 cut(s) 79, 302
SlaI CTCGAG 1 cut(s) 708
SmiMI CAYNNNNRTG 1 cut(s) 203
SmlI CTYRAG 2 cut(s) 335, 708
SmoI CTYRAG 2 cut(s) 335, 708
SsiI CCGC 2 cut(s) 42, 976
SspMI CTAG 2 cut(s) 501, 558
StyD4I CCNGG 4 cut(s) 161, 441, 528, 626
StyI CCWWGG 2 cut(s) 90, 570
TaaI ACNGT 2 cut(s) 39, 293
TaiI ACGT 3 cut(s) 174, 426, 927
TaqI TCGA 5 cut(s) 105, 402, 426, 594, 709
TfiI GAWTC 3 cut(s) 404, 454, 631
Tru1I TTAA 1 cut(s) 912
Tru9I TTAA 1 cut(s) 912
TscAI CASTG 1 cut(s) 42
TseFI GTSAC 4 cut(s) 33, 196, 925, 979
TseI GCWGC 1 cut(s) 268
Tsp45I GTSAC 4 cut(s) 33, 196, 925, 979
TspRI CASTG 1 cut(s) 42
Van91I CCANNNNNTGG 1 cut(s) 135
VpaK11BI GGWCC 2 cut(s) 79, 302
XapI RAATTY 4 cut(s) 416, 533, 752, 784
XcmI CCANNNNNNNNNTGG 1 cut(s) 132
XhoI CTCGAG 1 cut(s) 708
XmiI GTMKAC 1 cut(s) 426
XmnI GAANNNNTTC 2 cut(s) 99, 362
XspI CTAG 2 cut(s) 501, 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.