Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0005422

Physical Location & Seq

Genomic Coordinates Reverse (-)

2971349 .. 2976813

Gene Structure 5465 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1042700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2799 bp

ATGTCTTCCTCCTCCTCCTCCTCCCAATGTCTCTCTTCTCTTCTTCTAGTGCTGTTAATCCTCTGTTTATCTGGTGTGAGCTCAGCTGACACCGTTAACCCCACCATCAAGGGCAAAGGCTACCGTCTCATCTCCATCGAAGAAACCCCTGACGGCGGCCTTCTGGGTCTCCTCCAGCTCAAACAGAAGTCCAAAACCTACGGCCCCGATATCCCCCTCCTGCAGCTCTATGTCAAGCACGAGACACAGGACCGGTTGAGGGTCCACATAACCGACGCTCAGAAGCAGAGGTGGGAGGTTCCCTACAACCTCCTCCCCAGGGAGCAGCCGCCGTCTCTGAAACAAACCATCACGCCGTCAGGGAAGACGAAGAACCCCATCGAAGTCTCAGAGTACTCCGGCTCTGAGCTCATTCTCAGCTACATCTCCGACCCATTTGGGTTCGCAGTCAAGAGGAAGTCCGACGGGCAGGTCCTCTTCAATTCGAGCTCCGAATCGAAAGACCCATATGGCGAACTGGTGTTTAAGGACCAGTACCTCGAGATATCCACCCGACTGCCGAAAGACGCCTCCCTGTACGGCCTCGGAGAGAACTCTCAGCCGCACGGGATCAAGCTCTACCCGAACGATCCATACACCCTCTTCACCACCGACGTCTCGGCGATCAATCTCAACACTGATTTGTACGGGTCCCACCCGGTTTACATGGATCTCCGGAATGTGGGCAGCGAGGCTTACGCACACTCTGTTCTGCTGCTCAACAGCAATGGCATGGATGTGTTTTACAGAGGGACTTCTCTCACCTACAAGGTCATTGGGGGTGTTTTCGACTTTTACTTCTTTGCTGGGCCGACGCCGTTGGGCGTTGTCGACCAGTACACGGCGTTCATCGGCAGACCGGCCCCGATGCCCTACTGGTCTCTTGGATTCCACCAATGCAGATGGGGTTACCACAACTTATCAGTGGTTGAAGATGTTGTTGCGAACTACAAAAAGGCTGAAATCCCGCTAGATGTCATTTGGAATGACGATGATCACATGGACGGACACAAAGACTTCACCCTCAACCCCAAAAACTACCCTCGTCCAAAGCTCTTGGCATTCCTTGACAAAATACATAAGATAGGCATGAAGTACATTGTCATTATCGATCCTGGAATTGGTGTTAATACCAGTTACGGTGTATACCAAAGAGGTTTAGCGAATGATGTTTTCATTAAATATGAGGGTGAACCCTACTTGGCCCAAGTTTGGCCTGGGGCTGTAAACTTCCCCGACTTCCTCAACCCCAAAACTGTTTCATGGTGGGGTGATGAGATCCGCCGCTTTCATGAACTTGTCCCTGTTGATGGCTTATGGATTGACATGAACGAAGCTTCAAATTTCTGTTCTGGGAAGTGCAAAATTCCAAACAAGCAGTGTCCGACGGGTACAGGACCTGGCTGGGTATGTTGCTTGGATTGCAAAAACATTACAAAGACAAGATGGGATGATCCACCTTACAAGATCAATGCTTCAGGGTTGCAGGTACCCATTGGATACAAAACCATAGCCACTAGTGCATCTCATTACAATGGCGTTTTGGAGTATGATGCTCACAGTCTCTATGGCTTTTCCCAGTCCATTGCAACTCACAAAGCTCTTCAAGGGCTTGAGGGCAAGAGGCCATTTATATTAACCCGGTCCACATATGTTGGTTCAGGCAGGTATGCTGCACATTGGACAGGTGATAACAAGGGGACTTGGGAGGATTTGAAGATATCAATCACTACCGTGCTCAATTTTGGAATATTTGGAGTGCCGATGGTTGGTGCAGACATATGTGGGTTCTATCCAGCACCTACTGAAGAGCTTTGCAACCGTTGGATTGAAGTAGGCGCTTTTTATCCCTTTTCAAGGGATCATGCGAACTTCTATTCCCCGAGACAAGAGCTTTATCAATGGGAGTCTGTAGCTGAGTCTGCTCGAAATGCACTAGGTATGAGGTATAAACTCCTCCCTTATCTGTACACATTAAGCTACGAAGCTCACATCAGCGGTGCCCCAATTGCCAGGCCACTTTTCTTCTCGTTCCCAACATATACTGAAACGTATGGATTGAGTACTCAATTCTTGTTGGGGAGCAGCGTTATGATTTCACCAGTGCTTGAACAGGGAAAGTCAAACGTTAAAGCATTATTTCCCCCGGGAAGCTGGTACAGTTTATTTGATATGACGCAGGCCATTAGCTCAACAAAAGGGAAGTATGTTACGCTTGATGCGCCTTTGCATGTTGTTAATGTGCATCTGTATCAGAATACCATTCTACCAATGCAGCAGGGTGGACTGATCTCTAAAGCAGCAAGAACAACCCCTTTTAGCCTCGTTGTTGCGTTCCCAGCTGGGGCAAGCAATGCAACAGCCAAGGGGAACCTGTTCCTTGACAACGATGAGCTTCCCGAGATGAATCTTGGAAACGGTTACTCCACATATGTTGATTTCTATGCAACTGTAAGCGAAGGAAATGTGAAAGTGTGGTCAGAAGTTCAGGAGGGTAAGTTTGCTTTAAGTAAAGGCTGGATTGTTGGGAAGGTGAGTGTGTTAGGACTGGATGGAAGCGGAGCAACATCTTCTCTCGAGGTTGATGGAAACCAAGCGACAAGCGTTTCAAACGTAGAGTTGATTGCTTCAGAACAGACGTATCTCAATGAGATAGAAGACGGAGAGCAGACCAAGAGCGTAATGGTGGAGGTTAACGGTCTGTCCCTTCCTGTTGGGAAGAACTTTGCCATGTCTTGGAAAATGGGGATCAAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005911 GO:0005975 GO:0005976 GO:0006073 GO:0008150 GO:0008152 GO:0009044 GO:0009056 GO:0009057 GO:0009505 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0010383 GO:0010410 GO:0010411 GO:0016052 GO:0016787 GO:0016798 GO:0030054 GO:0030312 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045493 GO:0046556 GO:0046686 GO:0048046 GO:0050896 GO:0055044 GO:0071554 GO:0071704 GO:0071944 GO:0080176 GO:0097599 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00052 ko00500 ko01100 map00052 map00500 map01100

Pfam Domains

Protein Families

Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

933

Amino Acids

103.22

Weight (kDa)

5.93

Isoelectric Point (pI)

36.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gal_mutarotas_2	PF13802	85 - 244	6.5e-10	Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd	PF01055	300 - 672	2.6e-121	Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd	PF21365	680 - 769	1.3e-18	Glycosyl hydrolase family 31 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005422)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68560 AT3G45940
fragaria_vesca	FvH4_4g27500
malus_domestica	MD13G1042700.v1.1 MD16G1043700.v1.1
prunus_persica	Prupe.1G309900_v2.0.a1 Prupe.2G051500_v2.0.a1 Prupe.2G076500_v2.0.a1
pyrus_communis	pycom13g03740 pycom16g03770
rosa_chinensis	RchiOBHm_Chr4g0435281 RchiOBHm_Chr4g0435321
rosa_laevigata	RLG00000006601
rosa_multiflora	Rmu_sc0000357.1_g000002 Rmu_sc0001940.1_g000004 Rmu_sc0002132.1_g000062
rosa_roxburghii	Rroxscaffold_5G00376570 Rroxscaffold_5G00376580
rosa_rugosa	Rorug04G0284200
rosa_samantha	Rh4AG339600 Rh4AG339800 Rh4BG348000 Rh4BG348300 Rh4CG362600 Rh4CG362900 Rh4DG342000 Rh4DG342100
rosa_wichuraiana	Rw4G029630 Rw4G029640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	470
AatII	GACGTC	1 cut(s)	657
Acc36I	ACCTGC	3 cut(s)	460, 1516, 1695
Acc65I	GGTACC	1 cut(s)	1528
AccB1I	GGYRCC	2 cut(s)	1528, 2039
AccB7I	CCANNNNNTGG	1 cut(s)	2775
AccI	GTMKAC	2 cut(s)	870, 1185
AccIII	TCCGGA	1 cut(s)	714
AciI	CCGC	8 cut(s)	156, 329, 602, 1007, 1321, 1324, 2037, 2598
AclI	AACGTT	1 cut(s)	2166
AclWI	GGATC	8 cut(s)	617, 623, 717, 1145, 1312, 1487, 1908, 2795
AcsI	RAATTY	2 cut(s)	1381, 1404
AcuI	CTGAAG	3 cut(s)	1500, 1866, 2652
AcyI	GRCGYC	3 cut(s)	567, 654, 854
AgeI	ACCGGT	1 cut(s)	252
AgsI	TTSAA	9 cut(s)	481, 971, 1380, 1646, 1756, 1871, 1896, 2150, 2649
AhlI	ACTAGT	1 cut(s)	1556
AjnI	CCWGG	5 cut(s)	317, 1153, 1255, 1438, 2051
AleI	CACNNNNGTG	1 cut(s)	1772
AloI	GAACNNNNNNTCC	2 cut(s)	1901, 1933
Alw21I	GWGCWC	4 cut(s)	83, 411, 491, 1779
AlwI	GGATC	8 cut(s)	617, 623, 717, 1145, 1312, 1487, 1908, 2795
AlwNI	CAGNNNCTG	1 cut(s)	1439
Ama87I	CYCGRG	5 cut(s)	539, 1921, 2185, 2438, 2615
Aor13HI	TCCGGA	1 cut(s)	714
ApeKI	GCWGC	8 cut(s)	223, 325, 726, 754, 1712, 2124, 2314, 2339
ApoI	RAATTY	2 cut(s)	1381, 1404
AsiGI	ACCGGT	1 cut(s)	252
Asp700I	GAANNNNTTC	1 cut(s)	2414
Asp718I	GGTACC	1 cut(s)	1528
AspLEI	GCGC	2 cut(s)	1880, 2263
AsuC2I	CCSGG	4 cut(s)	698, 1681, 2186, 2187
AsuHPI	GGTGA	8 cut(s)	637, 793, 1051, 1241, 1322, 1739, 2130, 2584
AvaI	CYCGRG	5 cut(s)	539, 1921, 2185, 2438, 2615
AvaII	GGWCC	7 cut(s)	250, 262, 472, 529, 690, 1436, 1683
BaeGI	GKGCMC	1 cut(s)	2044
BaeI	ACNNNNGTAYC	2 cut(s)	2663, 2696
BanI	GGYRCC	2 cut(s)	1528, 2039
BanII	GRGCYC	3 cut(s)	83, 411, 491
BauI	CACGAG	1 cut(s)	239
BbsI	GAAGAC	2 cut(s)	371, 2703
Bbv12I	GWGCWC	4 cut(s)	83, 411, 491, 1779
BbvI	GCAGC	8 cut(s)	235, 337, 738, 741, 1699, 2136, 2326, 2351
BceAI	ACGGC	7 cut(s)	169, 217, 316, 340, 595, 841, 897
BciT130I	CCWGG	5 cut(s)	319, 1155, 1257, 1440, 2053
BciVI	GTATCC	1 cut(s)	1532
BclI	TGATCA	1 cut(s)	1033
BcnI	CCSGG	4 cut(s)	698, 1681, 2186, 2187
BcuI	ACTAGT	1 cut(s)	1556
BfaI	CTAG	4 cut(s)	47, 1010, 1557, 1976
BfmI	CTRYAG	2 cut(s)	221, 1950
BfoI	RGCGCY	1 cut(s)	1881
BfuAI	ACCTGC	3 cut(s)	460, 1516, 1695
BfuI	GTATCC	1 cut(s)	1532
BlpI	GCTNAGC	1 cut(s)	82
BmcAI	AGTACT	2 cut(s)	395, 2104
Bme1390I	CCNGG	9 cut(s)	319, 698, 1155, 1257, 1440, 1681, 2053, 2186, 2187
Bme18I	GGWCC	7 cut(s)	250, 262, 472, 529, 690, 1436, 1683
BmeT110I	CYCGRG	5 cut(s)	539, 1921, 2185, 2438, 2615
BmiI	GGNNCC	9 cut(s)	205, 263, 300, 691, 692, 903, 1530, 2041, 2411
BmrFI	CCNGG	9 cut(s)	319, 698, 1155, 1257, 1440, 1681, 2053, 2186, 2187
BmrI	ACTGGG	1 cut(s)	1612
BmsI	GCATC	5 cut(s)	897, 1571, 1582, 2248, 2293
BmuI	ACTGGG	1 cut(s)	1612
BpiI	GAAGAC	2 cut(s)	371, 2703
BpmI	CTGGAG	1 cut(s)	158
Bpu1102I	GCTNAGC	1 cut(s)	82
BpuEI	CTTGAG	1 cut(s)	1673
BpuMI	CCSGG	4 cut(s)	698, 1681, 2186, 2187
Bsa29I	ATCGAT	1 cut(s)	1149
BsaBI	GATNNNNATC	1 cut(s)	1763
BsaHI	GRCGYC	3 cut(s)	567, 654, 854
BsaI	GGTCTC	2 cut(s)	173, 924
BsaJI	CCNNGG	7 cut(s)	317, 318, 583, 1256, 2184, 2185, 2403
BsaWI	WCCGGW	2 cut(s)	252, 714
BsaXI	ACNNNNNCTCC	4 cut(s)	2447, 2477, 2522, 2552
Bse118I	RCCGGY	2 cut(s)	252, 898
Bse1I	ACTGG	8 cut(s)	522, 532, 874, 920, 1173, 1618, 2141, 2592
Bse3DI	GCAATG	3 cut(s)	772, 1623, 2398
Bse8I	GATNNNNATC	1 cut(s)	1763
BseAI	TCCGGA	1 cut(s)	714
BseBI	CCWGG	5 cut(s)	319, 1155, 1257, 1440, 2053
BseCI	ATCGAT	1 cut(s)	1149
BseDI	CCNNGG	7 cut(s)	317, 318, 583, 1256, 2184, 2185, 2403
BseGI	GGATG	3 cut(s)	781, 1495, 2596
BseJI	GATNNNNATC	1 cut(s)	1763
BseMI	GCAATG	3 cut(s)	772, 1623, 2398
BseMII	CTCAG	7 cut(s)	96, 293, 396, 402, 430, 611, 1947
BseNI	ACTGG	8 cut(s)	522, 532, 874, 920, 1173, 1618, 2141, 2592
BseRI	GAGGAG	6 cut(s)	4, 7, 10, 161, 302, 1985
BseSI	GKGCMC	1 cut(s)	2044
BseXI	GCAGC	8 cut(s)	235, 337, 738, 741, 1699, 2136, 2326, 2351
BseYI	CCCAGC	4 cut(s)	845, 1443, 2377, 2381
BsgI	GTGCAG	2 cut(s)	1698, 1833
BshNI	GGYRCC	2 cut(s)	1528, 2039
BshTI	ACCGGT	1 cut(s)	252
BshVI	ATCGAT	1 cut(s)	1149
BsiHKAI	GWGCWC	4 cut(s)	83, 411, 491, 1779
BsiHKCI	CYCGRG	5 cut(s)	539, 1921, 2185, 2438, 2615
BsiSI	CCGG	7 cut(s)	253, 399, 698, 715, 899, 1681, 2186
BslFI	GGGAC	5 cut(s)	676, 805, 1325, 1753, 2728
BsmBI	CGTCTC	3 cut(s)	131, 339, 661
BsmFI	GGGAC	5 cut(s)	676, 805, 1325, 1753, 2728
BsmI	GAATGC	1 cut(s)	1100
Bso31I	GGTCTC	2 cut(s)	173, 924
BsoBI	CYCGRG	5 cut(s)	539, 1921, 2185, 2438, 2615
Bsp1286I	GDGCHC	5 cut(s)	83, 411, 491, 1779, 2044
Bsp13I	TCCGGA	1 cut(s)	714
Bsp1407I	TGTACA	1 cut(s)	2007
Bsp1720I	GCTNAGC	1 cut(s)	82
BspACI	CCGC	8 cut(s)	156, 329, 602, 1007, 1321, 1324, 2037, 2598
BspCNI	CTCAG	7 cut(s)	95, 292, 397, 401, 429, 610, 1948
BspDI	ATCGAT	1 cut(s)	1149
BspEI	TCCGGA	1 cut(s)	714
BspHI	TCATGA	1 cut(s)	1330
BspLI	GGNNCC	9 cut(s)	205, 263, 300, 691, 692, 903, 1530, 2041, 2411
BspMAI	CTGCAG	1 cut(s)	225
BspMI	ACCTGC	3 cut(s)	460, 1516, 1695
BspPI	GGATC	8 cut(s)	617, 623, 717, 1145, 1312, 1487, 1908, 2795
BspQI	GCTCTTC	2 cut(s)	1647, 1842
BspT107I	GGYRCC	2 cut(s)	1528, 2039
BspTNI	GGTCTC	2 cut(s)	173, 924
BsrDI	GCAATG	3 cut(s)	772, 1623, 2398
BsrFI	RCCGGY	2 cut(s)	252, 898
BsrGI	TGTACA	1 cut(s)	2007
BsrI	ACTGG	8 cut(s)	522, 532, 874, 920, 1173, 1618, 2141, 2592
BssAI	RCCGGY	2 cut(s)	252, 898
BssECI	CCNNGG	7 cut(s)	317, 318, 583, 1256, 2184, 2185, 2403
BssNAI	GTATAC	1 cut(s)	1186
BssNI	GRCGYC	3 cut(s)	567, 654, 854
BssSI	CACGAG	1 cut(s)	239
BssT1I	CCWWGG	1 cut(s)	2403
Bst1107I	GTATAC	1 cut(s)	1186
Bst2BI	CACGAG	1 cut(s)	239
Bst2UI	CCWGG	5 cut(s)	319, 1155, 1257, 1440, 2053
Bst6I	CTCTTC	6 cut(s)	40, 45, 482, 647, 1647, 1842
BstACI	GRCGYC	3 cut(s)	567, 654, 854
BstAPI	GCANNNNNTGC	1 cut(s)	2393
BstAUI	TGTACA	1 cut(s)	2007
BstC8I	GCNNGC	2 cut(s)	2220, 2389
BstDEI	CTNAG	7 cut(s)	82, 279, 388, 405, 416, 597, 1956
BstEII	GGTNACC	1 cut(s)	947
BstENI	CCTNNNNNAGG	1 cut(s)	1894
BstF5I	GGATG	3 cut(s)	781, 1495, 2596
BstH2I	RGCGCY	1 cut(s)	1881
BstHHI	GCGC	2 cut(s)	1880, 2263
BstMWI	GCNNNNNNNGC	8 cut(s)	1461, 1559, 1961, 1970, 2048, 2260, 2378, 2393
BstNI	CCWGG	5 cut(s)	319, 1155, 1257, 1440, 2053
BstNSI	RCATGY	1 cut(s)	2273
BstPI	GGTNACC	1 cut(s)	947
BstSCI	CCNGG	9 cut(s)	317, 696, 1153, 1255, 1438, 1679, 2051, 2184, 2185
BstSFI	CTRYAG	2 cut(s)	221, 1950
BstSLI	GKGCMC	1 cut(s)	2044
BstV1I	GCAGC	8 cut(s)	235, 337, 738, 741, 1699, 2136, 2326, 2351
BstV2I	GAAGAC	2 cut(s)	371, 2703
BstX2I	RGATCY	2 cut(s)	709, 1317
BstYI	RGATCY	2 cut(s)	709, 1317
BstZ17I	GTATAC	1 cut(s)	1186
Bsu15I	ATCGAT	1 cut(s)	1149
BsuI	GTATCC	1 cut(s)	1532
BsuTUI	ATCGAT	1 cut(s)	1149
BtsCI	GGATG	3 cut(s)	781, 1495, 2596
BtsI	GCAGTG	1 cut(s)	1424
BtsIMutI	CAGTG	4 cut(s)	675, 969, 1424, 2148
BveI	ACCTGC	3 cut(s)	460, 1516, 1695
Cac8I	GCNNGC	2 cut(s)	2220, 2389
CaiI	CAGNNNCTG	1 cut(s)	1439
CciI	TCATGA	1 cut(s)	1330
CfoI	GCGC	2 cut(s)	1880, 2263
Cfr10I	RCCGGY	2 cut(s)	252, 898
Cfr9I	CCCGGG	1 cut(s)	2185
ClaI	ATCGAT	1 cut(s)	1149
CseI	GACGC	4 cut(s)	284, 575, 862, 2224
CspAI	ACCGGT	1 cut(s)	252
CspCI	CAANNNNNGTGG	4 cut(s)	1675, 1710, 2455, 2490
DdeI	CTNAG	7 cut(s)	82, 279, 388, 405, 416, 597, 1956
DrdI	GACNNNNNNGTC	1 cut(s)	470
DseDI	GACNNNNNNGTC	1 cut(s)	470
Eam1104I	CTCTTC	6 cut(s)	40, 45, 482, 647, 1647, 1842
EarI	CTCTTC	6 cut(s)	40, 45, 482, 647, 1647, 1842
EciI	GGCGGA	1 cut(s)	1310
Ecl136II	GAGCTC	3 cut(s)	81, 409, 489
Eco130I	CCWWGG	1 cut(s)	2403
Eco24I	GRGCYC	3 cut(s)	83, 411, 491
Eco31I	GGTCTC	2 cut(s)	173, 924
Eco32I	GATATC	3 cut(s)	211, 546, 1761
Eco47I	GGWCC	7 cut(s)	250, 262, 472, 529, 690, 1436, 1683
Eco53kI	GAGCTC	3 cut(s)	81, 409, 489
Eco57I	CTGAAG	3 cut(s)	1500, 1866, 2652
Eco88I	CYCGRG	5 cut(s)	539, 1921, 2185, 2438, 2615
Eco91I	GGTNACC	1 cut(s)	947
EcoICRI	GAGCTC	3 cut(s)	81, 409, 489
EcoNI	CCTNNNNNAGG	1 cut(s)	1894
EcoO109I	RGGNCCY	3 cut(s)	472, 690, 1436
EcoO65I	GGTNACC	1 cut(s)	947
EcoRII	CCWGG	5 cut(s)	317, 1153, 1255, 1438, 2051
EcoRV	GATATC	3 cut(s)	211, 546, 1761
EcoT14I	CCWWGG	1 cut(s)	2403
EcoT38I	GRGCYC	3 cut(s)	83, 411, 491
ErhI	CCWWGG	1 cut(s)	2403
Esp3I	CGTCTC	3 cut(s)	131, 339, 661
FaqI	GGGAC	5 cut(s)	676, 805, 1325, 1753, 2728
FauI	CCCGC	1 cut(s)	1014
FauNDI	CATATG	4 cut(s)	508, 1690, 1820, 2470
FbaI	TGATCA	1 cut(s)	1033
FblI	GTMKAC	2 cut(s)	870, 1185
FokI	GGATG	3 cut(s)	788, 1502, 2603
FriOI	GRGCYC	3 cut(s)	83, 411, 491
FspBI	CTAG	4 cut(s)	47, 1010, 1557, 1976
GlaI	GCGC	2 cut(s)	1879, 2262
GsaI	CCCAGC	4 cut(s)	849, 1447, 2381, 2385
GsuI	CTGGAG	1 cut(s)	158
HaeII	RGCGCY	1 cut(s)	1881
HapII	CCGG	7 cut(s)	253, 399, 698, 715, 899, 1681, 2186
HgaI	GACGC	4 cut(s)	284, 575, 862, 2224
HhaI	GCGC	2 cut(s)	1880, 2263
Hin1I	GRCGYC	3 cut(s)	567, 654, 854
Hin6I	GCGC	2 cut(s)	1878, 2261
HinP1I	GCGC	2 cut(s)	1878, 2261
HincII	GTYRAC	3 cut(s)	97, 871, 2734
HindII	GTYRAC	3 cut(s)	97, 871, 2734
HindIII	AAGCTT	1 cut(s)	1374
HinfI	GANTC	5 cut(s)	494, 927, 1946, 1958, 2446
HpaI	GTTAAC	2 cut(s)	97, 2734
HpaII	CCGG	7 cut(s)	253, 399, 698, 715, 899, 1681, 2186
HphI	GGTGA	8 cut(s)	637, 793, 1051, 1241, 1322, 1739, 2130, 2584
Hpy188III	TCNNGA	7 cut(s)	451, 541, 715, 1331, 2438, 2528, 2615
Hpy99I	CGWCG	5 cut(s)	278, 467, 656, 856, 1429
HpyAV	CCTTC	4 cut(s)	170, 2492, 2563, 2756
HpyCH4IV	ACGT	5 cut(s)	654, 2090, 2166, 2652, 2678
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1461, 1559, 1961, 1970, 2048, 2260, 2378, 2393
HpyF3I	CTNAG	7 cut(s)	82, 279, 388, 405, 416, 597, 1956
HpySE526I	ACGT	5 cut(s)	654, 2090, 2166, 2652, 2678
Hsp92I	GRCGYC	3 cut(s)	567, 654, 854
HspAI	GCGC	2 cut(s)	1878, 2261
KflI	GGGWCCC	1 cut(s)	690
Kpn2I	TCCGGA	1 cut(s)	714
KpnI	GGTACC	1 cut(s)	1532
Ksp22I	TGATCA	1 cut(s)	1033
KspAI	GTTAAC	2 cut(s)	97, 2734
LguI	GCTCTTC	2 cut(s)	1647, 1842
LmnI	GCTCC	4 cut(s)	322, 494, 2121, 2600
Lsp1109I	GCAGC	8 cut(s)	235, 337, 738, 741, 1699, 2136, 2326, 2351
LweI	GCATC	5 cut(s)	897, 1571, 1582, 2248, 2293
MaeI	CTAG	4 cut(s)	47, 1010, 1557, 1976
MaeII	ACGT	5 cut(s)	654, 2090, 2166, 2652, 2678
MaeIII	GTNAC	4 cut(s)	947, 1175, 2248, 2459
MfeI	CAATTG	1 cut(s)	2046
MflI	RGATCY	2 cut(s)	709, 1317
MhlI	GDGCHC	5 cut(s)	83, 411, 491, 1779, 2044
MluCI	AATT	7 cut(s)	481, 1158, 1381, 1404, 1780, 2046, 2108
MlyI	GAGTC	2 cut(s)	1955, 1967
MmeI	TCCRAC	4 cut(s)	453, 486, 1448, 1844
MroI	TCCGGA	1 cut(s)	714
MroXI	GAANNNNTTC	1 cut(s)	2414
MslI	CAYNNNNRTG	3 cut(s)	776, 1572, 1772
MspA1I	CMGCKG	3 cut(s)	86, 2037, 2381
MspI	CCGG	7 cut(s)	253, 399, 698, 715, 899, 1681, 2186
MspR9I	CCNGG	9 cut(s)	319, 698, 1155, 1257, 1440, 1681, 2053, 2186, 2187
MunI	CAATTG	1 cut(s)	2046
Mva1269I	GAATGC	1 cut(s)	1100
MvaI	CCWGG	5 cut(s)	319, 1155, 1257, 1440, 2053
MwoI	GCNNNNNNNGC	8 cut(s)	1461, 1559, 1961, 1970, 2048, 2260, 2378, 2393
NciI	CCSGG	4 cut(s)	698, 1681, 2186, 2187
NdeI	CATATG	4 cut(s)	508, 1690, 1820, 2470
NlaIV	GGNNCC	9 cut(s)	205, 263, 300, 691, 692, 903, 1530, 2041, 2411
NmeAIII	GCCGAG	1 cut(s)	638
NspI	RCATGY	1 cut(s)	2273
OliI	CACNNNNGTG	1 cut(s)	1772
PaeR7I	CTCGAG	2 cut(s)	539, 2615
PagI	TCATGA	1 cut(s)	1330
PasI	CCCWGGG	1 cut(s)	318
PciSI	GCTCTTC	2 cut(s)	1647, 1842
PctI	GAATGC	1 cut(s)	1100
PdmI	GAANNNNTTC	1 cut(s)	2414
PfeI	GAWTC	3 cut(s)	494, 927, 2446
PflMI	CCANNNNNTGG	1 cut(s)	2775
PfoI	TCCNGGA	1 cut(s)	1153
PinAI	ACCGGT	1 cut(s)	252
PleI	GAGTC	2 cut(s)	1954, 1966
PpsI	GAGTC	2 cut(s)	1954, 1966
PpuMI	RGGWCCY	3 cut(s)	472, 690, 1436
Psp124BI	GAGCTC	3 cut(s)	83, 411, 491
Psp1406I	AACGTT	1 cut(s)	2166
Psp5II	RGGWCCY	3 cut(s)	472, 690, 1436
Psp6I	CCWGG	5 cut(s)	317, 1153, 1255, 1438, 2051
PspEI	GGTNACC	1 cut(s)	947
PspFI	CCCAGC	4 cut(s)	845, 1443, 2377, 2381
PspGI	CCWGG	5 cut(s)	317, 1153, 1255, 1438, 2051
PspN4I	GGNNCC	9 cut(s)	205, 263, 300, 691, 692, 903, 1530, 2041, 2411
PspPPI	RGGWCCY	3 cut(s)	472, 690, 1436
PsrI	GAACNNNNNNTAC	4 cut(s)	617, 649, 869, 901
PstI	CTGCAG	1 cut(s)	225
PstNI	CAGNNNCTG	1 cut(s)	1439
PsuI	RGATCY	2 cut(s)	709, 1317
PvuII	CAGCTG	2 cut(s)	86, 2381
RseI	CAYNNNNRTG	3 cut(s)	776, 1572, 1772
SacI	GAGCTC	3 cut(s)	83, 411, 491
SalI	GTCGAC	1 cut(s)	869
SapI	GCTCTTC	2 cut(s)	1647, 1842
ScaI	AGTACT	2 cut(s)	395, 2104
SchI	GAGTC	2 cut(s)	1955, 1967
ScrFI	CCNGG	9 cut(s)	319, 698, 1155, 1257, 1440, 1681, 2053, 2186, 2187
SduI	GDGCHC	5 cut(s)	83, 411, 491, 1779, 2044
SfaNI	GCATC	5 cut(s)	897, 1571, 1582, 2248, 2293
SfcI	CTRYAG	2 cut(s)	221, 1950
Sfr274I	CTCGAG	2 cut(s)	539, 2615
SinI	GGWCC	7 cut(s)	250, 262, 472, 529, 690, 1436, 1683
SlaI	CTCGAG	2 cut(s)	539, 2615
SmaI	CCCGGG	1 cut(s)	2187
SmiMI	CAYNNNNRTG	3 cut(s)	776, 1572, 1772
SmlI	CTYRAG	3 cut(s)	539, 1652, 2615
SmoI	CTYRAG	3 cut(s)	539, 1652, 2615
SpeI	ACTAGT	1 cut(s)	1556
Sse9I	AATT	7 cut(s)	481, 1158, 1381, 1404, 1780, 2046, 2108
SsiI	CCGC	8 cut(s)	156, 329, 602, 1007, 1321, 1324, 2037, 2598
SspI	AATATT	1 cut(s)	1791
SspMI	CTAG	4 cut(s)	47, 1010, 1557, 1976
SstI	GAGCTC	3 cut(s)	83, 411, 491
StyD4I	CCNGG	9 cut(s)	317, 696, 1153, 1255, 1438, 1679, 2051, 2184, 2185
StyI	CCWWGG	1 cut(s)	2403
TaiI	ACGT	5 cut(s)	657, 2093, 2169, 2655, 2681
TasI	AATT	7 cut(s)	481, 1158, 1381, 1404, 1780, 2046, 2108
TatI	WGTACW	5 cut(s)	393, 876, 1134, 2007, 2102
TauI	GCSGC	4 cut(s)	159, 331, 604, 1326
TfiI	GAWTC	3 cut(s)	494, 927, 2446
TscAI	CASTG	4 cut(s)	682, 969, 1424, 2148
TseI	GCWGC	8 cut(s)	223, 325, 726, 754, 1712, 2124, 2314, 2339
TspDTI	ATGAA	8 cut(s)	877, 1145, 1204, 1290, 1319, 1347, 1382, 2459
TspGWI	ACGGA	2 cut(s)	1059, 2715
TspMI	CCCGGG	1 cut(s)	2185
TspRI	CASTG	4 cut(s)	682, 969, 1424, 2148
Van91I	CCANNNNNTGG	1 cut(s)	2775
VpaK11BI	GGWCC	7 cut(s)	250, 262, 472, 529, 690, 1436, 1683
XagI	CCTNNNNNAGG	1 cut(s)	1894
XapI	RAATTY	2 cut(s)	1381, 1404
XceI	RCATGY	1 cut(s)	2273
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1253, 2719
XhoI	CTCGAG	2 cut(s)	539, 2615
XmaI	CCCGGG	1 cut(s)	2185
XmiI	GTMKAC	2 cut(s)	870, 1185
XmnI	GAANNNNTTC	1 cut(s)	2414
XspI	CTAG	4 cut(s)	47, 1010, 1557, 1976
ZraI	GACGTC	1 cut(s)	655
ZrmI	AGTACT	2 cut(s)	395, 2104

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.