Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0005422

Physical Location & Seq

Genomic Coordinates Reverse (-)

3070047 .. 3075265

Gene Structure 5219 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1043700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2793 bp

ATGTCATCCTCTTCCTCCCAATGTATCTCTTCTCTTCTTCTACTTCTCCTTGTCCTCTGTTTCTCTGGTGTGAGCTCAGCTGACACCGTTAACCCCACCATCAAAGGCAAAGGCTACCGTCTCATCTCCATCGACGATACCCCTGACGGCGGCCTTCTGGGTCTCCTCCAGCTCAAACAGGAGTCCAAAACCTACGGCCCCGATGTCCCACTCTTGCAGCTATATGTCAAGCACGAGACGCAGGACCGGTTGAGGGTTCACATAACTGACGCGCAGAAACAGAGGTGGGAGGTTCCGTACAACCTCCTCCCCAGAGAGCAGCCGCCGTCGCTGAAACAAACCATCACGCCGTCAGGGAAGACGAAGAACCCCATCCAGCACTCAGAATACTCCGGCTCCGATCTCATCTTCAGCTACATCTCCGACCCATTTGGGTTCGCCATCAAGAGGAAGTCCGACGGGCAGGTCCTCTTCAATTCGAGCTCCGAATCGAAAGACCCATATGGCGAATTGGTGTTTAAGGACCAGTACCTCGAGATTTCCACCAGTTTGCCGAAAGACGCCGCTCTGTACGGCCTGGGGGAGAACTCGCAGCCGCACGGGATCAAGCTCTACCCGAACGATCCCTACACCCTCTTCACCACCGACATCTCGGCGATCAATCTCAACACTGATTTGTACGGCTCCCACCCGGTTTATATGGATCTCCGGAATGTGGGTGGCGAGGCGTACGCACACTCTGTTCTGCTGCTCAACAGCAATGGCATGGATGTGTTTTACAGAGGGACTTCTCTGACCTACAAGGTCATTGGGGGTGTTTTCGACTTTTACTTCTTTTCTGGGCCGACGCCATTGGGCGTTGTCGACCAGTACACGGCGTTCGTCGGCCGACCGGCTCCGATGCCCTACTGGTCTCTTGGATTCCACCAATGCAGATGGGGTTACCACAACTTATCAGTAGTTGAAGACGTTGTTGCAAACTACGAAAAGGCTCAAATCCCGCTAGATGTCATTTGGAATGATGATGATCACATGGATGGACACAAAGACTTCACCCTCAACCCCGAGAATTACCCTCGCCCAAAGCTTTTGGCATTCCTCGACAAAATACATAAGATAGGCATGAAGTACATTGTCATTATCGATCCTGGAATTGCTGTTAATAGCAGTTACGGTGTGTACCAGAGAGGTTTAGCGAATGATGTTTTCATCAAATACGAGGGTGAACCCTACTTGGCCCAAGTTTGGCCGGGGGCTGTAAACTTCCCCGACTTCCTCAACCCCAAAACTGTTTCATGGTGGGGTGAAGAGATCCGCCGTTTTCATGAACTTGTCCCTGTTGATGGCTTATGGATTGACATGAATGAGGCTTCAAATTTCTGTTCTGGGAAGTGCAAAATTCCAAATAAGCAGTGTCCGACAGGTACAGGCCCTGGCTGGGATTGTTGCTTGGATTGCAAGAACATTACAAAGACGAGATGGGATGATCCACCTTACAAGATCAACGCTTCAGGGTCGCAAGCCCCCATTGGGTTCAAAACCATAGCCACTAGTGCATCTCATTACAATGGCGTTTTGGAGTACGATGCTCACAGTCTCTATGGATTTTCCCAGTCCATTGCAACTCACAAAGCTCTTCAAGGACTTGAGGGCAAGAGACCGTTTATATTAACCCGGTCTACGTATGTTGGTTCAGGCAGGTATGCTGCACATTGGACAGGTGATAACAAGGGGACATGGGATGATTTGAAGATATCAATCACAACTGTGCTCAATTTTGGAATATTTGGAGTGCCGATGGTTGGTGCAGACATATGCGGGTTCTATCCAGCACCTACTGAAGAGCTTTGCAACCGTTGGATTGAAGTAGGGGCTTTCTATCCCTTTTCAAGGGATCACGCGAACTTCTATTCCCCAAGACAAGAGCTTTATCAATGGGAGTCGGTAGCTGAGTCTGCTCGAAATGCTCTAGGTATGAGGTATAAGCTCCTCCCCTATCTGTACACATTGAGCTACGAAGCTCATATCAGCGGAGCCCCAATTGCCAGGCCACTTTTCTTCTCATTCCCAGCATACACTGAAACGTATGGATTGAGTACTCAATTCTTGTTGGGGAGCAGCGTTATGATTTCACCGGTGCTTGAACAGGGCAAATCAGAAGTTAAAGCATTATTTCCCCCGGGAAGCTGGTACAGTTTATTTGATATGACGCAGATCATTAGCTCAACAAATGGGGAGTATGTTACGCTTGATGCGCCTTTGCATGTTGTTAATGTGCATCTGTATCAGAATACCATCATACCAATGCAGCAGGGTGGACTGATCTCTAAAGCAGCAAGAACAACCCCTTTTAGCCTCGTTGTCGCGTTCCCAGCTGGGGCAAGCAATGCAACAGCCAAGGGGAACCTGTTCCTTGACAATGATGAGCTTCCCGAGATGAAGCTTGGAAACGGTTACTCCACGTATGTTGACTTCTATGCAACTGTAAGTGAAGGAATTGTTAAAGTATGGTCAGAAGTTCAGGACGGTAACTTTGCTTTAAGTAAAGGCTGGATTATTGGGAAGGTGAGTGTATTGGGACTGGATGGAAGCGGAGGAACATCTGCTCTCGAGATTGATGGAGACCAAATGACAAGCATTTCAAACATAGAGTTGATTGCTTCAGAACAGACGTATCTCAATGAGATAGAAGACGGAGGGAAGACCAAGAGCACAGTGGTGGAGGTTGACGGTCTGTCCTTTCCTGTTGGGAAGAACTTTGCCATGTCCTGGAAAGTGGGGACCAAGCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005911 GO:0005975 GO:0005976 GO:0006073 GO:0008150 GO:0008152 GO:0009044 GO:0009056 GO:0009057 GO:0009505 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0010383 GO:0010410 GO:0010411 GO:0016052 GO:0016787 GO:0016798 GO:0030054 GO:0030312 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045493 GO:0046556 GO:0046686 GO:0048046 GO:0050896 GO:0055044 GO:0071554 GO:0071704 GO:0071944 GO:0080176 GO:0097599 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00052 ko00500 ko01100 map00052 map00500 map01100

Pfam Domains

Protein Families

Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

931

Amino Acids

102.95

Weight (kDa)

5.42

Isoelectric Point (pI)

34.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gal_mutarotas_2	PF13802	83 - 244	1.9e-09	Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd	PF01055	298 - 670	1.2e-121	Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd	PF21365	678 - 767	1e-18	Glycosyl hydrolase family 31 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005422)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68560 AT3G45940
fragaria_vesca	FvH4_4g27500
malus_domestica	MD13G1042700.v1.1 MD16G1043700.v1.1
prunus_persica	Prupe.1G309900_v2.0.a1 Prupe.2G051500_v2.0.a1 Prupe.2G076500_v2.0.a1
pyrus_communis	pycom13g03740 pycom16g03770
rosa_chinensis	RchiOBHm_Chr4g0435281 RchiOBHm_Chr4g0435321
rosa_laevigata	RLG00000006601
rosa_multiflora	Rmu_sc0000357.1_g000002 Rmu_sc0001940.1_g000004 Rmu_sc0002132.1_g000062
rosa_roxburghii	Rroxscaffold_5G00376570 Rroxscaffold_5G00376580
rosa_rugosa	Rorug04G0284200
rosa_samantha	Rh4AG339600 Rh4AG339800 Rh4BG348000 Rh4BG348300 Rh4CG362600 Rh4CG362900 Rh4DG342000 Rh4DG342100
rosa_wichuraiana	Rw4G029630 Rw4G029640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	464
Acc36I	ACCTGC	2 cut(s)	454, 1689
AccB7I	CCANNNNNTGG	1 cut(s)	2769
AccBSI	CCGCTC	1 cut(s)	566
AccI	GTMKAC	2 cut(s)	864, 1679
AccII	CGCG	3 cut(s)	272, 1901, 2366
AccIII	TCCGGA	1 cut(s)	708
AciI	CCGC	9 cut(s)	150, 323, 564, 596, 1001, 1315, 1818, 2031, 2592
AclWI	GGATC	7 cut(s)	611, 617, 711, 1139, 1306, 1481, 1902
AcoI	YGGCCR	2 cut(s)	886, 1247
AcsI	RAATTY	2 cut(s)	1375, 1398
AcuI	CTGAAG	4 cut(s)	394, 1494, 1860, 2646
AcyI	GRCGYC	2 cut(s)	561, 848
AgeI	ACCGGT	2 cut(s)	246, 2134
AhdI	GACNNNNNGTC	1 cut(s)	2734
AhlI	ACTAGT	1 cut(s)	1550
AjnI	CCWGG	5 cut(s)	576, 1147, 1432, 2045, 2768
AleI	CACNNNNGTG	2 cut(s)	1766, 2717
AloI	GAACNNNNNNTCC	2 cut(s)	1895, 1927
Alw21I	GWGCWC	4 cut(s)	77, 485, 1773, 2714
Alw26I	GTCTC	7 cut(s)	125, 167, 230, 918, 1601, 1651, 2616
AlwI	GGATC	7 cut(s)	611, 617, 711, 1139, 1306, 1481, 1902
AlwNI	CAGNNNCTG	1 cut(s)	1433
Ama87I	CYCGRG	5 cut(s)	533, 1064, 2179, 2432, 2609
Aor13HI	TCCGGA	1 cut(s)	708
AoxI	GGCC	9 cut(s)	151, 196, 574, 842, 886, 1236, 1247, 1429, 2048
ApeKI	GCWGC	8 cut(s)	217, 319, 592, 748, 1706, 2118, 2308, 2333
ApoI	RAATTY	2 cut(s)	1375, 1398
ArsI	GACNNNNNNTTYG	2 cut(s)	2516, 2548
AsiGI	ACCGGT	2 cut(s)	246, 2134
Asp700I	GAANNNNTTC	1 cut(s)	2408
AspLEI	GCGC	2 cut(s)	274, 2257
AspS9I	GGNCC	8 cut(s)	197, 244, 466, 523, 842, 1237, 1430, 2781
AsuC2I	CCSGG	5 cut(s)	692, 1251, 1675, 2180, 2181
AsuHPI	GGTGA	7 cut(s)	631, 1045, 1235, 1316, 1733, 2124, 2578
AvaI	CYCGRG	5 cut(s)	533, 1064, 2179, 2432, 2609
AvaII	GGWCC	4 cut(s)	244, 466, 523, 2781
BaeI	ACNNNNGTAYC	2 cut(s)	2657, 2690
BanII	GRGCYC	3 cut(s)	77, 485, 2038
BarI	GAAGNNNNNNTAC	2 cut(s)	2456, 2488
BauI	CACGAG	1 cut(s)	233
BbsI	GAAGAC	4 cut(s)	365, 972, 2697, 2708
Bbv12I	GWGCWC	4 cut(s)	77, 485, 1773, 2714
BbvI	GCAGC	8 cut(s)	229, 331, 604, 735, 1693, 2130, 2320, 2345
BceAI	ACGGC	8 cut(s)	163, 211, 310, 334, 589, 697, 891, 1302
BciT130I	CCWGG	5 cut(s)	578, 1149, 1434, 2047, 2770
BclI	TGATCA	1 cut(s)	1027
BcnI	CCSGG	5 cut(s)	692, 1251, 1675, 2180, 2181
BcoDI	GTCTC	7 cut(s)	125, 167, 230, 918, 1601, 1651, 2616
BcuI	ACTAGT	1 cut(s)	1550
BfaI	CTAG	3 cut(s)	1004, 1551, 1970
BfuAI	ACCTGC	2 cut(s)	454, 1689
BlpI	GCTNAGC	1 cut(s)	76
BmcAI	AGTACT	1 cut(s)	2098
Bme18I	GGWCC	4 cut(s)	244, 466, 523, 2781
BmeRI	GACNNNNNGTC	1 cut(s)	2734
BmeT110I	CYCGRG	5 cut(s)	533, 1064, 2179, 2432, 2609
BmgT120I	GGNCC	8 cut(s)	197, 244, 466, 523, 842, 1237, 1430, 2781
BmiI	GGNNCC	8 cut(s)	199, 294, 397, 685, 897, 2035, 2405, 2782
BmrI	ACTGGG	1 cut(s)	1606
BmsI	GCATC	5 cut(s)	891, 1565, 1576, 2242, 2287
BmuI	ACTGGG	1 cut(s)	1606
BpiI	GAAGAC	4 cut(s)	365, 972, 2697, 2708
BpmI	CTGGAG	1 cut(s)	152
Bpu1102I	GCTNAGC	1 cut(s)	76
BpuEI	CTTGAG	1 cut(s)	1667
BpuMI	CCSGG	5 cut(s)	692, 1251, 1675, 2180, 2181
Bsa29I	ATCGAT	1 cut(s)	1143
BsaAI	YACGTR	2 cut(s)	1683, 2463
BsaBI	GATNNNNATC	2 cut(s)	1026, 1757
BsaHI	GRCGYC	2 cut(s)	561, 848
BsaI	GGTCTC	4 cut(s)	167, 918, 1651, 2616
BsaJI	CCNNGG	6 cut(s)	577, 1250, 1432, 2178, 2179, 2397
BsaWI	WCCGGW	3 cut(s)	246, 708, 2134
BsaXI	ACNNNNNCTCC	6 cut(s)	281, 311, 380, 410, 2441, 2471
Bse118I	RCCGGY	3 cut(s)	246, 892, 2134
Bse1I	ACTGG	6 cut(s)	526, 546, 868, 914, 1612, 2586
Bse3DI	GCAATG	3 cut(s)	766, 1617, 2392
Bse8I	GATNNNNATC	2 cut(s)	1026, 1757
BseAI	TCCGGA	1 cut(s)	708
BseBI	CCWGG	5 cut(s)	578, 1149, 1434, 2047, 2770
BseCI	ATCGAT	1 cut(s)	1143
BseDI	CCNNGG	6 cut(s)	577, 1250, 1432, 2178, 2179, 2397
BseGI	GGATG	7 cut(s)	5, 372, 775, 1042, 1489, 1747, 2590
BseJI	GATNNNNATC	2 cut(s)	1026, 1757
BseMI	GCAATG	3 cut(s)	766, 1617, 2392
BseMII	CTCAG	3 cut(s)	90, 396, 1941
BseNI	ACTGG	6 cut(s)	526, 546, 868, 914, 1612, 2586
BseRI	GAGGAG	3 cut(s)	155, 296, 1979
BseX3I	CGGCCG	1 cut(s)	886
BseXI	GCAGC	8 cut(s)	229, 331, 604, 735, 1693, 2130, 2320, 2345
BseYI	CCCAGC	4 cut(s)	1437, 2068, 2371, 2375
BsgI	GTGCAG	2 cut(s)	1692, 1827
Bsh1236I	CGCG	3 cut(s)	272, 1901, 2366
Bsh1285I	CGRYCG	2 cut(s)	889, 893
BshFI	GGCC	9 cut(s)	153, 198, 576, 844, 888, 1238, 1249, 1431, 2050
BshTI	ACCGGT	2 cut(s)	246, 2134
BshVI	ATCGAT	1 cut(s)	1143
BsiEI	CGRYCG	2 cut(s)	889, 893
BsiHKAI	GWGCWC	4 cut(s)	77, 485, 1773, 2714
BsiHKCI	CYCGRG	5 cut(s)	533, 1064, 2179, 2432, 2609
BsiSI	CCGG	9 cut(s)	247, 393, 692, 709, 893, 1250, 1675, 2135, 2180
BsiWI	CGTACG	1 cut(s)	729
BslFI	GGGAC	5 cut(s)	191, 799, 1319, 1747, 2592
BsmAI	GTCTC	7 cut(s)	125, 167, 230, 918, 1601, 1651, 2616
BsmBI	CGTCTC	2 cut(s)	125, 230
BsmFI	GGGAC	5 cut(s)	191, 799, 1319, 1747, 2592
BsmI	GAATGC	1 cut(s)	1094
BsnI	GGCC	9 cut(s)	153, 198, 576, 844, 888, 1238, 1249, 1431, 2050
Bso31I	GGTCTC	4 cut(s)	167, 918, 1651, 2616
BsoBI	CYCGRG	5 cut(s)	533, 1064, 2179, 2432, 2609
Bsp1286I	GDGCHC	5 cut(s)	77, 485, 1773, 2038, 2714
Bsp13I	TCCGGA	1 cut(s)	708
Bsp1407I	TGTACA	1 cut(s)	2001
Bsp1720I	GCTNAGC	1 cut(s)	76
BspACI	CCGC	9 cut(s)	150, 323, 564, 596, 1001, 1315, 1818, 2031, 2592
BspANI	GGCC	9 cut(s)	153, 198, 576, 844, 888, 1238, 1249, 1431, 2050
BspCNI	CTCAG	3 cut(s)	89, 395, 1942
BspDI	ATCGAT	1 cut(s)	1143
BspEI	TCCGGA	1 cut(s)	708
BspFNI	CGCG	3 cut(s)	272, 1901, 2366
BspHI	TCATGA	1 cut(s)	1324
BspLI	GGNNCC	8 cut(s)	199, 294, 397, 685, 897, 2035, 2405, 2782
BspMI	ACCTGC	2 cut(s)	454, 1689
BspPI	GGATC	7 cut(s)	611, 617, 711, 1139, 1306, 1481, 1902
BspQI	GCTCTTC	2 cut(s)	1641, 1836
BspTNI	GGTCTC	4 cut(s)	167, 918, 1651, 2616
BsrBI	CCGCTC	1 cut(s)	566
BsrDI	GCAATG	3 cut(s)	766, 1617, 2392
BsrFI	RCCGGY	3 cut(s)	246, 892, 2134
BsrGI	TGTACA	1 cut(s)	2001
BsrI	ACTGG	6 cut(s)	526, 546, 868, 914, 1612, 2586
BssAI	RCCGGY	3 cut(s)	246, 892, 2134
BssECI	CCNNGG	6 cut(s)	577, 1250, 1432, 2178, 2179, 2397
BssNI	GRCGYC	2 cut(s)	561, 848
BssSI	CACGAG	1 cut(s)	233
BssT1I	CCWWGG	1 cut(s)	2397
Bst2BI	CACGAG	1 cut(s)	233
Bst2UI	CCWGG	5 cut(s)	578, 1149, 1434, 2047, 2770
Bst6I	CTCTTC	8 cut(s)	16, 34, 39, 476, 641, 1302, 1641, 1836
BstACI	GRCGYC	2 cut(s)	561, 848
BstAPI	GCANNNNNTGC	1 cut(s)	2387
BstAUI	TGTACA	1 cut(s)	2001
BstBAI	YACGTR	2 cut(s)	1683, 2463
BstC8I	GCNNGC	2 cut(s)	1521, 2383
BstDEI	CTNAG	3 cut(s)	76, 382, 1950
BstEII	GGTNACC	1 cut(s)	941
BstENI	CCTNNNNNAGG	1 cut(s)	1888
BstF5I	GGATG	7 cut(s)	5, 372, 775, 1042, 1489, 1747, 2590
BstFNI	CGCG	3 cut(s)	272, 1901, 2366
BstHHI	GCGC	2 cut(s)	274, 2257
BstMAI	GTCTC	7 cut(s)	125, 167, 230, 918, 1601, 1651, 2616
BstMCI	CGRYCG	2 cut(s)	889, 893
BstNI	CCWGG	5 cut(s)	578, 1149, 1434, 2047, 2770
BstNSI	RCATGY	1 cut(s)	2267
BstPI	GGTNACC	1 cut(s)	941
BstSNI	TACGTA	1 cut(s)	1683
BstUI	CGCG	3 cut(s)	272, 1901, 2366
BstV1I	GCAGC	8 cut(s)	229, 331, 604, 735, 1693, 2130, 2320, 2345
BstV2I	GAAGAC	4 cut(s)	365, 972, 2697, 2708
BstX2I	RGATCY	2 cut(s)	703, 1311
BstYI	RGATCY	2 cut(s)	703, 1311
BstZI	CGGCCG	1 cut(s)	886
Bsu15I	ATCGAT	1 cut(s)	1143
BsuRI	GGCC	9 cut(s)	153, 198, 576, 844, 888, 1238, 1249, 1431, 2050
BsuTUI	ATCGAT	1 cut(s)	1143
BtsCI	GGATG	7 cut(s)	5, 372, 775, 1042, 1489, 1747, 2590
BtsI	GCAGTG	1 cut(s)	1418
BtsIMutI	CAGTG	4 cut(s)	669, 1418, 2076, 2721
BveI	ACCTGC	2 cut(s)	454, 1689
Cac8I	GCNNGC	2 cut(s)	1521, 2383
CaiI	CAGNNNCTG	1 cut(s)	1433
CciI	TCATGA	1 cut(s)	1324
CfoI	GCGC	2 cut(s)	274, 2257
Cfr10I	RCCGGY	3 cut(s)	246, 892, 2134
Cfr13I	GGNCC	8 cut(s)	197, 244, 466, 523, 842, 1237, 1430, 2781
Cfr9I	CCCGGG	1 cut(s)	2179
ClaI	ATCGAT	1 cut(s)	1143
CseI	GACGC	5 cut(s)	247, 278, 569, 856, 2218
CspAI	ACCGGT	2 cut(s)	246, 2134
CspCI	CAANNNNNGTGG	2 cut(s)	2449, 2484
CviAII	CATG	9 cut(s)	766, 1033, 1123, 1296, 1325, 1360, 1737, 2264, 2764
DdeI	CTNAG	3 cut(s)	76, 382, 1950
DrdI	GACNNNNNNGTC	1 cut(s)	464
DriI	GACNNNNNGTC	1 cut(s)	2734
DseDI	GACNNNNNNGTC	1 cut(s)	464
EaeI	YGGCCR	2 cut(s)	886, 1247
EagI	CGGCCG	1 cut(s)	886
Eam1104I	CTCTTC	8 cut(s)	16, 34, 39, 476, 641, 1302, 1641, 1836
Eam1105I	GACNNNNNGTC	1 cut(s)	2734
EarI	CTCTTC	8 cut(s)	16, 34, 39, 476, 641, 1302, 1641, 1836
EciI	GGCGGA	1 cut(s)	1304
Ecl136II	GAGCTC	2 cut(s)	75, 483
EclXI	CGGCCG	1 cut(s)	886
Eco105I	TACGTA	1 cut(s)	1683
Eco130I	CCWWGG	1 cut(s)	2397
Eco24I	GRGCYC	3 cut(s)	77, 485, 2038
Eco31I	GGTCTC	4 cut(s)	167, 918, 1651, 2616
Eco32I	GATATC	1 cut(s)	1755
Eco47I	GGWCC	4 cut(s)	244, 466, 523, 2781
Eco52I	CGGCCG	1 cut(s)	886
Eco53kI	GAGCTC	2 cut(s)	75, 483
Eco57I	CTGAAG	4 cut(s)	394, 1494, 1860, 2646
Eco88I	CYCGRG	5 cut(s)	533, 1064, 2179, 2432, 2609
Eco91I	GGTNACC	1 cut(s)	941
EcoICRI	GAGCTC	2 cut(s)	75, 483
EcoNI	CCTNNNNNAGG	1 cut(s)	1888
EcoO109I	RGGNCCY	2 cut(s)	466, 1430
EcoO65I	GGTNACC	1 cut(s)	941
EcoRII	CCWGG	5 cut(s)	576, 1147, 1432, 2045, 2768
EcoRV	GATATC	1 cut(s)	1755
EcoT14I	CCWWGG	1 cut(s)	2397
EcoT38I	GRGCYC	3 cut(s)	77, 485, 2038
ErhI	CCWWGG	1 cut(s)	2397
Esp3I	CGTCTC	2 cut(s)	125, 230
FaeI	CATG	9 cut(s)	769, 1036, 1126, 1299, 1328, 1363, 1740, 2267, 2767
FaqI	GGGAC	5 cut(s)	191, 799, 1319, 1747, 2592
FatI	CATG	9 cut(s)	765, 1032, 1122, 1295, 1324, 1359, 1736, 2263, 2763
FauI	CCCGC	2 cut(s)	1008, 1811
FauNDI	CATATG	2 cut(s)	502, 1814
FbaI	TGATCA	1 cut(s)	1027
FblI	GTMKAC	2 cut(s)	864, 1679
FokI	GGATG	6 cut(s)	359, 782, 1049, 1496, 1754, 2597
FriOI	GRGCYC	3 cut(s)	77, 485, 2038
FspBI	CTAG	3 cut(s)	1004, 1551, 1970
GlaI	GCGC	2 cut(s)	273, 2256
GsaI	CCCAGC	4 cut(s)	1441, 2072, 2375, 2379
GsuI	CTGGAG	1 cut(s)	152
HaeIII	GGCC	9 cut(s)	153, 198, 576, 844, 888, 1238, 1249, 1431, 2050
HapII	CCGG	9 cut(s)	247, 393, 692, 709, 893, 1250, 1675, 2135, 2180
HgaI	GACGC	5 cut(s)	247, 278, 569, 856, 2218
HhaI	GCGC	2 cut(s)	274, 2257
Hin1I	GRCGYC	2 cut(s)	561, 848
Hin1II	CATG	9 cut(s)	769, 1036, 1126, 1299, 1328, 1363, 1740, 2267, 2767
Hin6I	GCGC	2 cut(s)	272, 2255
HinP1I	GCGC	2 cut(s)	272, 2255
HincII	GTYRAC	4 cut(s)	91, 865, 2470, 2728
HindII	GTYRAC	4 cut(s)	91, 865, 2470, 2728
HindIII	AAGCTT	2 cut(s)	1085, 2441
HinfI	GANTC	5 cut(s)	182, 488, 921, 1940, 1952
HpaI	GTTAAC	1 cut(s)	91
HpaII	CCGG	9 cut(s)	247, 393, 692, 709, 893, 1250, 1675, 2135, 2180
HphI	GGTGA	7 cut(s)	631, 1045, 1235, 1316, 1733, 2124, 2578
Hpy188III	TCNNGA	8 cut(s)	445, 535, 709, 1325, 2432, 2522, 2609, 2611
Hpy99I	CGWCG	5 cut(s)	137, 331, 461, 850, 887
HpyAV	CCTTC	3 cut(s)	164, 2486, 2557
HpyCH4IV	ACGT	5 cut(s)	969, 1682, 2084, 2462, 2672
HpyF3I	CTNAG	3 cut(s)	76, 382, 1950
HpySE526I	ACGT	5 cut(s)	969, 1682, 2084, 2462, 2672
Hsp92I	GRCGYC	2 cut(s)	561, 848
Hsp92II	CATG	9 cut(s)	769, 1036, 1126, 1299, 1328, 1363, 1740, 2267, 2767
HspAI	GCGC	2 cut(s)	272, 2255
Kpn2I	TCCGGA	1 cut(s)	708
Ksp22I	TGATCA	1 cut(s)	1027
KspAI	GTTAAC	1 cut(s)	91
LguI	GCTCTTC	2 cut(s)	1641, 1836
LmnI	GCTCC	7 cut(s)	401, 488, 689, 901, 1992, 2033, 2115
Lsp1109I	GCAGC	8 cut(s)	229, 331, 604, 735, 1693, 2130, 2320, 2345
LweI	GCATC	5 cut(s)	891, 1565, 1576, 2242, 2287
MaeI	CTAG	3 cut(s)	1004, 1551, 1970
MaeII	ACGT	5 cut(s)	969, 1682, 2084, 2462, 2672
MaeIII	GTNAC	5 cut(s)	941, 1169, 2242, 2453, 2528
MbiI	CCGCTC	1 cut(s)	566
MfeI	CAATTG	1 cut(s)	2040
MflI	RGATCY	2 cut(s)	703, 1311
MhlI	GDGCHC	5 cut(s)	77, 485, 1773, 2038, 2714
MlyI	GAGTC	3 cut(s)	191, 1949, 1961
MmeI	TCCRAC	4 cut(s)	447, 480, 1442, 1838
MroI	TCCGGA	1 cut(s)	708
MroXI	GAANNNNTTC	1 cut(s)	2408
MseI	TTAA	9 cut(s)	90, 519, 1161, 1670, 2163, 2271, 2502, 2540, 2791
MslI	CAYNNNNRTG	6 cut(s)	770, 1035, 1566, 1766, 2303, 2717
MspA1I	CMGCKG	3 cut(s)	80, 2031, 2375
MspI	CCGG	9 cut(s)	247, 393, 692, 709, 893, 1250, 1675, 2135, 2180
MunI	CAATTG	1 cut(s)	2040
Mva1269I	GAATGC	1 cut(s)	1094
MvaI	CCWGG	5 cut(s)	578, 1149, 1434, 2047, 2770
MvnI	CGCG	3 cut(s)	272, 1901, 2366
NciI	CCSGG	5 cut(s)	692, 1251, 1675, 2180, 2181
NdeI	CATATG	2 cut(s)	502, 1814
NlaIII	CATG	9 cut(s)	769, 1036, 1126, 1299, 1328, 1363, 1740, 2267, 2767
NlaIV	GGNNCC	8 cut(s)	199, 294, 397, 685, 897, 2035, 2405, 2782
NmeAIII	GCCGAG	1 cut(s)	632
NspI	RCATGY	1 cut(s)	2267
OliI	CACNNNNGTG	2 cut(s)	1766, 2717
PaeR7I	CTCGAG	2 cut(s)	533, 2609
PagI	TCATGA	1 cut(s)	1324
PciSI	GCTCTTC	2 cut(s)	1641, 1836
PctI	GAATGC	1 cut(s)	1094
PdmI	GAANNNNTTC	1 cut(s)	2408
PfeI	GAWTC	2 cut(s)	488, 921
Pfl23II	CGTACG	1 cut(s)	729
PflMI	CCANNNNNTGG	1 cut(s)	2769
PfoI	TCCNGGA	2 cut(s)	1147, 2768
PinAI	ACCGGT	2 cut(s)	246, 2134
PleI	GAGTC	3 cut(s)	190, 1948, 1960
PpsI	GAGTC	3 cut(s)	190, 1948, 1960
Ppu21I	YACGTR	2 cut(s)	1683, 2463
PpuMI	RGGWCCY	1 cut(s)	466
Psp124BI	GAGCTC	2 cut(s)	77, 485
Psp5II	RGGWCCY	1 cut(s)	466
Psp6I	CCWGG	5 cut(s)	576, 1147, 1432, 2045, 2768
PspEI	GGTNACC	1 cut(s)	941
PspFI	CCCAGC	4 cut(s)	1437, 2068, 2371, 2375
PspGI	CCWGG	5 cut(s)	576, 1147, 1432, 2045, 2768
PspLI	CGTACG	1 cut(s)	729
PspN4I	GGNNCC	8 cut(s)	199, 294, 397, 685, 897, 2035, 2405, 2782
PspPI	GGNCC	8 cut(s)	197, 244, 466, 523, 842, 1237, 1430, 2781
PspPPI	RGGWCCY	1 cut(s)	466
PsrI	GAACNNNNNNTAC	4 cut(s)	611, 643, 863, 895
PstNI	CAGNNNCTG	1 cut(s)	1433
PsuI	RGATCY	2 cut(s)	703, 1311
PvuII	CAGCTG	2 cut(s)	80, 2375
RseI	CAYNNNNRTG	6 cut(s)	770, 1035, 1566, 1766, 2303, 2717
SacI	GAGCTC	2 cut(s)	77, 485
SalI	GTCGAC	1 cut(s)	863
SapI	GCTCTTC	2 cut(s)	1641, 1836
SaqAI	TTAA	9 cut(s)	90, 519, 1161, 1670, 2163, 2271, 2502, 2540, 2791
Sau96I	GGNCC	8 cut(s)	197, 244, 466, 523, 842, 1237, 1430, 2781
ScaI	AGTACT	1 cut(s)	2098
SchI	GAGTC	3 cut(s)	191, 1949, 1961
SduI	GDGCHC	5 cut(s)	77, 485, 1773, 2038, 2714
SfaNI	GCATC	5 cut(s)	891, 1565, 1576, 2242, 2287
Sfr274I	CTCGAG	2 cut(s)	533, 2609
SgrAI	CRCCGGYG	1 cut(s)	2134
SinI	GGWCC	4 cut(s)	244, 466, 523, 2781
SlaI	CTCGAG	2 cut(s)	533, 2609
SmaI	CCCGGG	1 cut(s)	2181
SmiMI	CAYNNNNRTG	6 cut(s)	770, 1035, 1566, 1766, 2303, 2717
SmlI	CTYRAG	3 cut(s)	533, 1646, 2609
SmoI	CTYRAG	3 cut(s)	533, 1646, 2609
SnaBI	TACGTA	1 cut(s)	1683
SpeI	ACTAGT	1 cut(s)	1550
SsiI	CCGC	9 cut(s)	150, 323, 564, 596, 1001, 1315, 1818, 2031, 2592
SspI	AATATT	1 cut(s)	1785
SspMI	CTAG	3 cut(s)	1004, 1551, 1970
SstI	GAGCTC	2 cut(s)	77, 485
StyI	CCWWGG	1 cut(s)	2397
TaiI	ACGT	5 cut(s)	972, 1685, 2087, 2465, 2675
TatI	WGTACW	4 cut(s)	870, 1128, 2001, 2096
TauI	GCSGC	4 cut(s)	153, 325, 566, 598
TfiI	GAWTC	2 cut(s)	488, 921
Tru1I	TTAA	9 cut(s)	90, 519, 1161, 1670, 2163, 2271, 2502, 2540, 2791
Tru9I	TTAA	9 cut(s)	90, 519, 1161, 1670, 2163, 2271, 2502, 2540, 2791
TscAI	CASTG	4 cut(s)	676, 1418, 2083, 2721
TseI	GCWGC	8 cut(s)	217, 319, 592, 748, 1706, 2118, 2308, 2333
TspDTI	ATGAA	7 cut(s)	1139, 1198, 1284, 1313, 1341, 1376, 2453
TspGWI	ACGGA	2 cut(s)	285, 2709
TspMI	CCCGGG	1 cut(s)	2179
TspRI	CASTG	4 cut(s)	676, 1418, 2083, 2721
Van91I	CCANNNNNTGG	1 cut(s)	2769
VpaK11BI	GGWCC	4 cut(s)	244, 466, 523, 2781
XagI	CCTNNNNNAGG	1 cut(s)	1888
XapI	RAATTY	2 cut(s)	1375, 1398
XceI	RCATGY	1 cut(s)	2267
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2713
XhoI	CTCGAG	2 cut(s)	533, 2609
XmaI	CCCGGG	1 cut(s)	2179
XmiI	GTMKAC	2 cut(s)	864, 1679
XmnI	GAANNNNTTC	1 cut(s)	2408
XspI	CTAG	3 cut(s)	1004, 1551, 1970
ZrmI	AGTACT	1 cut(s)	2098

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.