Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0005422

Physical Location & Seq

Genomic Coordinates Reverse (-)

56315975 .. 56321727

Gene Structure 5753 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G029630.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2781 bp

ATGTGGTCTTCTACCACCACACTATGCCTCTGCTCCCTTCTCCTAGTTTTACTTCTCTGCTCCTCCGGTGCAAACTCTTACACCAACCCCAAGATCATCGGCAAAGGCTACCGTCTCATCTCCATTGAAGAAACCCACGACGGCGGTATTCTCGGCCACCTCCAGCTCAAACAGAAGTCCAAAACCTACGGCCCCGACATCCCACTCTTACAGCTCTTTGTCAAGCACGAGACGGATCAACGGCTGAGGGTCCACATTACTGATGCTCAGAAGCAGCGGTGGGAGGTGCCGTACAACCTCTTACCCAGAGAGCAGCCTCCGTCGCTGAAGCAGACCATTGGGAGGTCAAAGGATCCCATAACAGTCTCCGAGTACTCAAGCTCGGAGCTCGTCTTCAGCTACACCGCAGACCCGTTCGGGTTTGCGGTGAAGCGAAAGTCAGACAAGCAACTACTGTTCAACACCAGCTCTGATTCTTCAGACCCTTATGGGGAGATGGTGTTCAAGGACCAGTATCTTGAGATCTCCACAAAACTTCCCAAAGATGCATCCCTCTATGGGTTGGGAGAGAACTCCCAGCCCCATGGGATCAAGCTCTTCCCAAATGAACCTTATACCCTCTACACCACTGATGTCTCTGCTATCAATCTCAACACTGATTTGTATGGCTCCCACCCAGTTTACATGGATCTGAGGAATGTGGGTGGTGAGGCTTATGCTCATGCTGTTCTGTTGCTCAACAGTAATGGCATGGATGTGTTTTACAGAGGCAATTCTTTGACATATAAGGCCATTGGGGGTGTCTTTGATTTCTACTTCTTTTCTGGGACTAGTCCACTGGCTGTGGTTGACCAGTACACTTCTTTCATTGGCAGACCAGCTCCAATGCCTTACTGGTCTCTAGGATTCCACCAATGCAGATGGGGTTACCACAATTTATCAGTGGTTGAAGATGTTGTTGCTAATTATGAAAAGGCTCAAATCCCTCTAGATGTCATGTGGACTGATGATGATCACATGGACGTGCGCAAAGACTTCACTCTCAGCGCCATGAACTTCCCCCGGCCAAAGCTTCTGGCATTCCTAGACAAAATTCATAAGATTGGCATGAAGTACGTTGTCATTATCGATCCTGGAATTGGTGTTAATAGCACGTATGGTGTGTACCAAAGAGGTCTTGCTAATGATGTCTTCATCAAGTATGATAATGAACCTTACTTGGCCCAGGTTTGGCCAGGGGCTGTAAACTTCCCTGACTTTCTCAATCCAAAAACTGTTTCATGGTGGAGTGATGAGATCAAGCGCTTTCATGAACTTGTCCCTGTTGATGGGTTATGGATTGACATGAACGAGGCTTCAAATTTCTGTTCTGGGAAATGCACAATTCCAAAGGGGACGACGTGCCCTACTCCGGGTATACCTGGCTGGATATGTTGCTTGGATTGCAAGAACATTACAAAGACGAGATGGGATGATCCACCTTACAAGATAAATGCTTCAGGGTTGCAAGTCCCCTTAGGGTTCAAAACCATTGCCACTAGTGCATATCATTACAATGGCGTTTTGGAGTACGATGCTCACAGTCTGTATGGTTTTTCCCAAACCATTGCAACTCATCAAGGTCTTCAAGGGATTGCAGGCAAGAGGCCGTTCATATTAACCCGCTCCACTTATGTTGGTTCAGGCAAGTATGCTGCACATTGGACAGGTGATAATAAGGGAACTTGGGAGGATTTGAAGATATCAATCTCTACTGTCCTCAATTTTGGAATATTTGGGGTGCCGATGGTTGGCGCAGATATATGTGGCTTCTATCCAGCTCCCACTGAAGAGCTTTGCAATCGTTGGATTGAAGTAGGTGCTTTCTACCCCTTTTCAAGGGATCATGCAAACTTTGCTTCCCCAAGGCAAGAGCTTTATCAGTGGGAGTCGGTAGCTAAGTCTGCTAGAAATGCTCTTGGTATGAGGTATAAGCTCCTTCCTTATCTCTACACTTTGACCTATTTAGCTCATATCAGTGGAGCTCCAATTGCAAGGCCACTTTTCTTCTCATTCCCAACATATACTGAATGCTATGGCTTGAGTACTCAATTCTTGCTAGGGAGCGGTCTTCTGATCTCTCCAGTTGTTGAGGAAGGAAAATCTGAGGTTAAGGCATTGTTTCCCCCTGGAAGCTGGTACAGTTTATTTGATATGACACAGGCTGTTAATTCAAAGGGACAGTATGTTACACTTGATGCGCCTTTGCATGTAGTTAATGTACATTTGTATCAGAACATCATTCTACCAATGCAGCAGGGTGGAACCGTGTCTAAAGAAGCGAGAAAGACTCCTTTTAGCCTCGTAGTAACCTTCCCAGCTGGGGAAAGCAATGCAACAGCGAAGGGGAACATTTTCATTGATGACGATGAGCTTCCAGAAATGAAACTCGGAAGTGGCTACTCCACATATGTTGATCTCTATGCAACTGTAAGTCAAGGATATGTGAAAGTATGGTCAGAAGTTCAGGAGGGAAAATTTGCCCTAGACCAAGGTTTGATTGTTGAGAAGGTGAGCGTGCTGGGATTGGATGGAACTGGAGGAGCATCAGCTCTTGAGGTTGATGGAACCACAGTGACTAGTGTTTCGAAGATAGAGCTGAGCACATTTGAGCAGGAGTACCAAGAGGAGGTGGAAGATGGAGAGAACAAGACCAAGAGTGTAATGGTCCAGGTCAGTGGTCTATCACTTCCTGTGGGGAAGAACTTTGCCATGTCCTGGAAAATGAGTGCCAAAGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005576 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005911 GO:0005975 GO:0005976 GO:0006073 GO:0008150 GO:0008152 GO:0009044 GO:0009056 GO:0009057 GO:0009505 GO:0009506 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0010383 GO:0010410 GO:0010411 GO:0016052 GO:0016787 GO:0016798 GO:0030054 GO:0030312 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045491 GO:0045493 GO:0046556 GO:0046686 GO:0048046 GO:0050896 GO:0055044 GO:0071554 GO:0071704 GO:0071944 GO:0080176 GO:0097599 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00052 ko00500 ko01100 map00052 map00500 map01100

Pfam Domains

Protein Families

Gal_mutarotas_2 Glyco_hydro_31 NtCtMGAM_N

Protein Analysis

926

Amino Acids

102.98

Weight (kDa)

5.78

Isoelectric Point (pI)

35.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gal_mutarotas_2	PF13802	81 - 248	1.3e-11	Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd	PF01055	293 - 666	7.2e-120	Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd	PF21365	674 - 762	6.1e-19	Glycosyl hydrolase family 31 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005422)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G68560 AT3G45940
fragaria_vesca	FvH4_4g27500
malus_domestica	MD13G1042700.v1.1 MD16G1043700.v1.1
prunus_persica	Prupe.1G309900_v2.0.a1 Prupe.2G051500_v2.0.a1 Prupe.2G076500_v2.0.a1
pyrus_communis	pycom13g03740 pycom16g03770
rosa_chinensis	RchiOBHm_Chr4g0435281 RchiOBHm_Chr4g0435321
rosa_laevigata	RLG00000006601
rosa_multiflora	Rmu_sc0000357.1_g000002 Rmu_sc0001940.1_g000004 Rmu_sc0002132.1_g000062
rosa_roxburghii	Rroxscaffold_5G00376570 Rroxscaffold_5G00376580
rosa_rugosa	Rorug04G0284200
rosa_samantha	Rh4AG339600 Rh4AG339800 Rh4BG348000 Rh4BG348300 Rh4CG362600 Rh4CG362900 Rh4DG342000 Rh4DG342100
rosa_wichuraiana	Rw4G029630 Rw4G029640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1028
AccB1I	GGYRCC	2 cut(s)	286, 1780
AccB7I	CCANNNNNTGG	1 cut(s)	2757
AccBSI	CCGCTC	2 cut(s)	1665, 2106
AccI	GTMKAC	1 cut(s)	1417
AciI	CCGC	6 cut(s)	144, 277, 405, 425, 1663, 2106
AclWI	GGATC	8 cut(s)	243, 347, 360, 596, 696, 1124, 1469, 1890
AcoI	YGGCCR	3 cut(s)	154, 1064, 1232
AcsI	RAATTY	3 cut(s)	1092, 1360, 2516
AcuI	CTGAAG	5 cut(s)	347, 379, 462, 1482, 1848
AfeI	AGCGCT	1 cut(s)	1304
AhlI	ACTAGT	3 cut(s)	830, 1538, 2618
AjiI	CACGTC	2 cut(s)	1024, 1401
AjnI	CCWGG	7 cut(s)	1132, 1224, 1234, 1420, 2167, 2709, 2756
AjuI	GAANNNNNNNTTGG	2 cut(s)	1261, 1293
AloI	GAACNNNNNNTCC	2 cut(s)	485, 517
Alw21I	GWGCWC	3 cut(s)	390, 2026, 2645
Alw26I	GTCTC	5 cut(s)	119, 224, 370, 640, 903
AlwI	GGATC	8 cut(s)	243, 347, 360, 596, 696, 1124, 1469, 1890
AlwNI	CAGNNNCTG	1 cut(s)	1241
Aor51HI	AGCGCT	1 cut(s)	1304
AoxI	GGCC	8 cut(s)	154, 190, 789, 1064, 1221, 1232, 1646, 2036
ApeKI	GCWGC	4 cut(s)	274, 313, 1694, 2293
ApoI	RAATTY	3 cut(s)	1092, 1360, 2516
ArsI	GACNNNNNNTTYG	2 cut(s)	2608, 2640
Asp700I	GAANNNNTTC	1 cut(s)	2393
AspLEI	GCGC	5 cut(s)	1029, 1049, 1305, 1796, 2242
AspS9I	GGNCC	5 cut(s)	191, 250, 508, 1222, 2707
AsuC2I	CCSGG	2 cut(s)	1063, 1413
AsuHPI	GGTGA	4 cut(s)	439, 719, 1721, 2563
AsuII	TTCGAA	1 cut(s)	2627
AvaII	GGWCC	3 cut(s)	250, 508, 2707
AxyI	CCTNAGG	1 cut(s)	1516
BaeGI	GKGCMC	1 cut(s)	1406
BaeI	ACNNNNGTAYC	2 cut(s)	2252, 2285
BalI	TGGCCA	1 cut(s)	1234
BamHI	GGATCC	1 cut(s)	352
BanI	GGYRCC	2 cut(s)	286, 1780
BanII	GRGCYC	2 cut(s)	390, 2026
BauI	CACGAG	1 cut(s)	227
BbsI	GAAGAC	4 cut(s)	385, 1183, 1616, 2102
Bbv12I	GWGCWC	3 cut(s)	390, 2026, 2645
BbvCI	CCTCAGC	1 cut(s)	245
BbvI	GCAGC	4 cut(s)	286, 325, 1681, 2305
BccI	CCATC	8 cut(s)	490, 915, 1322, 1461, 1780, 2564, 2597, 2672
BceAI	ACGGC	5 cut(s)	157, 205, 257, 274, 1633
BciT130I	CCWGG	7 cut(s)	1134, 1226, 1236, 1422, 2169, 2711, 2758
BclI	TGATCA	1 cut(s)	1012
BcnI	CCSGG	2 cut(s)	1063, 1413
BcoDI	GTCTC	5 cut(s)	119, 224, 370, 640, 903
BcuI	ACTAGT	3 cut(s)	830, 1538, 2618
BfoI	RGCGCY	2 cut(s)	1050, 1306
BglII	AGATCT	1 cut(s)	522
BisI	GCNGC	4 cut(s)	275, 314, 1695, 2294
BlpI	GCTNAGC	1 cut(s)	2639
BlsI	GCNGC	4 cut(s)	276, 315, 1696, 2295
BmcAI	AGTACT	2 cut(s)	374, 2086
Bme1390I	CCNGG	9 cut(s)	1063, 1134, 1226, 1236, 1413, 1422, 2169, 2711, 2758
Bme18I	GGWCC	3 cut(s)	250, 508, 2707
BmgBI	CACGTC	2 cut(s)	1024, 1401
BmgT120I	GGNCC	5 cut(s)	191, 250, 508, 1222, 2707
BmiI	GGNNCC	8 cut(s)	193, 251, 288, 354, 670, 1782, 2305, 2608
BmrFI	CCNGG	9 cut(s)	1063, 1134, 1226, 1236, 1413, 1422, 2169, 2711, 2758
BmrI	ACTGGG	1 cut(s)	671
BmsI	GCATC	6 cut(s)	253, 535, 557, 1564, 2227, 2594
BmuI	ACTGGG	1 cut(s)	671
BpiI	GAAGAC	4 cut(s)	385, 1183, 1616, 2102
BplI	GAGNNNNNCTC	2 cut(s)	2314, 2346
BpmI	CTGGAG	3 cut(s)	146, 2106, 2598
Bpu10I	CCTNAGC	1 cut(s)	245
Bpu1102I	GCTNAGC	1 cut(s)	2639
Bpu14I	TTCGAA	1 cut(s)	2627
BpuEI	CTTGAG	4 cut(s)	361, 539, 2101, 2615
BpuMI	CCSGG	2 cut(s)	1063, 1413
Bsa29I	ATCGAT	1 cut(s)	1128
BsaAI	YACGTR	1 cut(s)	1155
BsaBI	GATNNNNATC	2 cut(s)	1011, 1745
BsaI	GGTCTC	1 cut(s)	903
BsaJI	CCNNGG	7 cut(s)	583, 1061, 1224, 1235, 1904, 2167, 2530
BsaWI	WCCGGW	1 cut(s)	65
BsaXI	ACNNNNNCTCC	4 cut(s)	275, 305, 485, 515
Bse1I	ACTGG	7 cut(s)	511, 677, 843, 853, 899, 2123, 2581
Bse21I	CCTNAGG	1 cut(s)	1516
Bse3DI	GCAATG	3 cut(s)	1530, 1605, 2377
Bse8I	GATNNNNATC	2 cut(s)	1011, 1745
BseBI	CCWGG	7 cut(s)	1134, 1226, 1236, 1422, 2169, 2711, 2758
BseCI	ATCGAT	1 cut(s)	1128
BseDI	CCNNGG	7 cut(s)	583, 1061, 1224, 1235, 1904, 2167, 2530
BseGI	GGATG	5 cut(s)	198, 548, 760, 1477, 2575
BseJI	GATNNNNATC	2 cut(s)	1011, 1745
BseMI	GCAATG	3 cut(s)	1530, 1605, 2377
BseMII	CTCAG	6 cut(s)	236, 281, 683, 1057, 2136, 2630
BseNI	ACTGG	7 cut(s)	511, 677, 843, 853, 899, 2123, 2581
BseRI	GAGGAG	3 cut(s)	52, 2595, 2681
BseSI	GKGCMC	1 cut(s)	1406
BseXI	GCAGC	4 cut(s)	286, 325, 1681, 2305
BseYI	CCCAGC	4 cut(s)	576, 2356, 2360, 2560
BsgI	GTGCAG	1 cut(s)	1680
BshFI	GGCC	8 cut(s)	156, 192, 791, 1066, 1223, 1234, 1648, 2038
BshNI	GGYRCC	2 cut(s)	286, 1780
BshVI	ATCGAT	1 cut(s)	1128
BsiHKAI	GWGCWC	3 cut(s)	390, 2026, 2645
BsiSI	CCGG	3 cut(s)	66, 1063, 1412
BslFI	GGGAC	5 cut(s)	841, 1304, 1408, 1496, 2232
BsmAI	GTCTC	5 cut(s)	119, 224, 370, 640, 903
BsmBI	CGTCTC	2 cut(s)	119, 224
BsmFI	GGGAC	5 cut(s)	841, 1304, 1408, 1496, 2232
BsmI	GAATGC	2 cut(s)	1079, 2075
BsnI	GGCC	8 cut(s)	156, 192, 791, 1066, 1223, 1234, 1648, 2038
Bso31I	GGTCTC	1 cut(s)	903
Bsp119I	TTCGAA	1 cut(s)	2627
Bsp1286I	GDGCHC	4 cut(s)	390, 1406, 2026, 2645
Bsp1407I	TGTACA	1 cut(s)	2260
Bsp1720I	GCTNAGC	1 cut(s)	2639
Bsp19I	CCATGG	1 cut(s)	583
BspACI	CCGC	6 cut(s)	144, 277, 405, 425, 1663, 2106
BspANI	GGCC	8 cut(s)	156, 192, 791, 1066, 1223, 1234, 1648, 2038
BspCNI	CTCAG	6 cut(s)	237, 280, 684, 1056, 2137, 2631
BspDI	ATCGAT	1 cut(s)	1128
BspHI	TCATGA	1 cut(s)	1309
BspLI	GGNNCC	8 cut(s)	193, 251, 288, 354, 670, 1782, 2305, 2608
BspPI	GGATC	8 cut(s)	243, 347, 360, 596, 696, 1124, 1469, 1890
BspQI	GCTCTTC	2 cut(s)	602, 1824
BspT104I	TTCGAA	1 cut(s)	2627
BspT107I	GGYRCC	2 cut(s)	286, 1780
BspTNI	GGTCTC	1 cut(s)	903
BsrBI	CCGCTC	2 cut(s)	1665, 2106
BsrDI	GCAATG	3 cut(s)	1530, 1605, 2377
BsrGI	TGTACA	1 cut(s)	2260
BsrI	ACTGG	7 cut(s)	511, 677, 843, 853, 899, 2123, 2581
BssECI	CCNNGG	7 cut(s)	583, 1061, 1224, 1235, 1904, 2167, 2530
BssNAI	GTATAC	1 cut(s)	1418
BssSI	CACGAG	1 cut(s)	227
BssT1I	CCWWGG	3 cut(s)	583, 1904, 2530
Bst1107I	GTATAC	1 cut(s)	1418
Bst2BI	CACGAG	1 cut(s)	227
Bst2UI	CCWGG	7 cut(s)	1134, 1226, 1236, 1422, 2169, 2711, 2758
Bst6I	CTCTTC	2 cut(s)	602, 1824
BstAPI	GCANNNNNTGC	1 cut(s)	1895
BstAUI	TGTACA	1 cut(s)	2260
BstBAI	YACGTR	1 cut(s)	1155
BstBI	TTCGAA	1 cut(s)	2627
BstC8I	GCNNGC	2 cut(s)	1639, 2558
BstDEI	CTNAG	8 cut(s)	245, 267, 692, 1043, 1516, 1938, 2145, 2639
BstDSI	CCRYGG	1 cut(s)	583
BstEII	GGTNACC	1 cut(s)	926
BstENI	CCTNNNNNAGG	1 cut(s)	1876
BstF5I	GGATG	5 cut(s)	198, 548, 760, 1477, 2575
BstH2I	RGCGCY	2 cut(s)	1050, 1306
BstHHI	GCGC	5 cut(s)	1029, 1049, 1305, 1796, 2242
BstMAI	GTCTC	5 cut(s)	119, 224, 370, 640, 903
BstMWI	GCNNNNNNNGC	7 cut(s)	322, 1443, 1541, 1895, 1943, 1952, 2030
BstNI	CCWGG	7 cut(s)	1134, 1226, 1236, 1422, 2169, 2711, 2758
BstNSI	RCATGY	1 cut(s)	2252
BstPI	GGTNACC	1 cut(s)	926
BstSCI	CCNGG	9 cut(s)	1061, 1132, 1224, 1234, 1411, 1420, 2167, 2709, 2756
BstSLI	GKGCMC	1 cut(s)	1406
BstV1I	GCAGC	4 cut(s)	286, 325, 1681, 2305
BstV2I	GAAGAC	4 cut(s)	385, 1183, 1616, 2102
BstX2I	RGATCY	3 cut(s)	352, 522, 688
BstXI	CCANNNNNNTGG	2 cut(s)	584, 2717
BstYI	RGATCY	3 cut(s)	352, 522, 688
BstZ17I	GTATAC	1 cut(s)	1418
Bsu15I	ATCGAT	1 cut(s)	1128
Bsu36I	CCTNAGG	1 cut(s)	1516
BsuRI	GGCC	8 cut(s)	156, 192, 791, 1066, 1223, 1234, 1648, 2038
BsuTUI	ATCGAT	1 cut(s)	1128
BtgI	CCRYGG	1 cut(s)	583
BtrI	CACGTC	2 cut(s)	1024, 1401
BtsCI	GGATG	5 cut(s)	198, 548, 760, 1477, 2575
BtsIMutI	CAGTG	9 cut(s)	627, 654, 836, 948, 1824, 1928, 2023, 2619, 2722
Cac8I	GCNNGC	2 cut(s)	1639, 2558
CaiI	CAGNNNCTG	1 cut(s)	1241
CciI	TCATGA	1 cut(s)	1309
CfoI	GCGC	5 cut(s)	1029, 1049, 1305, 1796, 2242
Cfr13I	GGNCC	5 cut(s)	191, 250, 508, 1222, 2707
ClaI	ATCGAT	1 cut(s)	1128
CspCI	CAANNNNNGTGG	4 cut(s)	1657, 1692, 2434, 2469
DdeI	CTNAG	8 cut(s)	245, 267, 692, 1043, 1516, 1938, 2145, 2639
EaeI	YGGCCR	3 cut(s)	154, 1064, 1232
Eam1104I	CTCTTC	2 cut(s)	602, 1824
EarI	CTCTTC	2 cut(s)	602, 1824
Ecl136II	GAGCTC	2 cut(s)	388, 2024
Eco130I	CCWWGG	3 cut(s)	583, 1904, 2530
Eco24I	GRGCYC	2 cut(s)	390, 2026
Eco31I	GGTCTC	1 cut(s)	903
Eco32I	GATATC	1 cut(s)	1743
Eco47I	GGWCC	3 cut(s)	250, 508, 2707
Eco47III	AGCGCT	1 cut(s)	1304
Eco53kI	GAGCTC	2 cut(s)	388, 2024
Eco57I	CTGAAG	5 cut(s)	347, 379, 462, 1482, 1848
Eco81I	CCTNAGG	1 cut(s)	1516
Eco91I	GGTNACC	1 cut(s)	926
EcoICRI	GAGCTC	2 cut(s)	388, 2024
EcoNI	CCTNNNNNAGG	1 cut(s)	1876
EcoO65I	GGTNACC	1 cut(s)	926
EcoRII	CCWGG	7 cut(s)	1132, 1224, 1234, 1420, 2167, 2709, 2756
EcoRV	GATATC	1 cut(s)	1743
EcoT14I	CCWWGG	3 cut(s)	583, 1904, 2530
EcoT22I	ATGCAT	1 cut(s)	550
EcoT38I	GRGCYC	2 cut(s)	390, 2026
ErhI	CCWWGG	3 cut(s)	583, 1904, 2530
Esp3I	CGTCTC	2 cut(s)	119, 224
FaqI	GGGAC	5 cut(s)	841, 1304, 1408, 1496, 2232
FauI	CCCGC	1 cut(s)	1670
FauNDI	CATATG	1 cut(s)	2449
FbaI	TGATCA	1 cut(s)	1012
FblI	GTMKAC	1 cut(s)	1417
Fnu4HI	GCNGC	4 cut(s)	275, 314, 1695, 2294
FokI	GGATG	5 cut(s)	185, 535, 767, 1484, 2582
FriOI	GRGCYC	2 cut(s)	390, 2026
Fsp4HI	GCNGC	4 cut(s)	275, 314, 1695, 2294
FspI	TGCGCA	1 cut(s)	1028
GlaI	GCGC	5 cut(s)	1028, 1048, 1304, 1795, 2241
GluI	GCNGC	4 cut(s)	275, 314, 1695, 2294
GsaI	CCCAGC	4 cut(s)	580, 2360, 2364, 2564
GsuI	CTGGAG	3 cut(s)	146, 2106, 2598
HaeII	RGCGCY	2 cut(s)	1050, 1306
HaeIII	GGCC	8 cut(s)	156, 192, 791, 1066, 1223, 1234, 1648, 2038
HapII	CCGG	3 cut(s)	66, 1063, 1412
HhaI	GCGC	5 cut(s)	1029, 1049, 1305, 1796, 2242
Hin6I	GCGC	5 cut(s)	1027, 1047, 1303, 1794, 2240
HinP1I	GCGC	5 cut(s)	1027, 1047, 1303, 1794, 2240
HincII	GTYRAC	1 cut(s)	850
HindII	GTYRAC	1 cut(s)	850
HindIII	AAGCTT	1 cut(s)	1070
HinfI	GANTC	4 cut(s)	473, 906, 1928, 2329
HpaII	CCGG	3 cut(s)	66, 1063, 1412
HphI	GGTGA	4 cut(s)	439, 719, 1721, 2563
Hpy166II	GTNNAC	9 cut(s)	253, 682, 836, 850, 858, 1002, 1165, 1246, 1418
Hpy188III	TCNNGA	6 cut(s)	518, 989, 1310, 2417, 2507, 2594
Hpy8I	GTNNAC	9 cut(s)	253, 682, 836, 850, 858, 1002, 1165, 1246, 1418
Hpy99I	CGWCG	3 cut(s)	143, 325, 1402
HpyAV	CCTTC	6 cut(s)	47, 1988, 2129, 2362, 2377, 2542
HpyCH4IV	ACGT	4 cut(s)	1023, 1116, 1154, 1400
HpyF10VI	GCNNNNNNNGC	7 cut(s)	322, 1443, 1541, 1895, 1943, 1952, 2030
HpyF3I	CTNAG	8 cut(s)	245, 267, 692, 1043, 1516, 1938, 2145, 2639
HpySE526I	ACGT	4 cut(s)	1023, 1116, 1154, 1400
HspAI	GCGC	5 cut(s)	1027, 1047, 1303, 1794, 2240
Ksp22I	TGATCA	1 cut(s)	1012
LguI	GCTCTTC	2 cut(s)	602, 1824
Lsp1109I	GCAGC	4 cut(s)	286, 325, 1681, 2305
LweI	GCATC	6 cut(s)	253, 535, 557, 1564, 2227, 2594
MaeII	ACGT	4 cut(s)	1023, 1116, 1154, 1400
MaeIII	GTNAC	4 cut(s)	926, 2227, 2347, 2614
MbiI	CCGCTC	2 cut(s)	1665, 2106
MfeI	CAATTG	1 cut(s)	2028
MflI	RGATCY	3 cut(s)	352, 522, 688
MhlI	GDGCHC	4 cut(s)	390, 1406, 2026, 2645
MlsI	TGGCCA	1 cut(s)	1234
MluNI	TGGCCA	1 cut(s)	1234
MlyI	GAGTC	2 cut(s)	1937, 2323
MmeI	TCCRAC	1 cut(s)	1826
Mox20I	TGGCCA	1 cut(s)	1234
Mph1103I	ATGCAT	1 cut(s)	550
MroXI	GAANNNNTTC	1 cut(s)	2393
MscI	TGGCCA	1 cut(s)	1234
MseI	TTAA	5 cut(s)	1146, 1658, 2151, 2208, 2256
MslI	CAYNNNNRTG	4 cut(s)	755, 1022, 1554, 2016
Msp20I	TGGCCA	1 cut(s)	1234
MspA1I	CMGCKG	2 cut(s)	277, 2360
MspI	CCGG	3 cut(s)	66, 1063, 1412
MspR9I	CCNGG	9 cut(s)	1063, 1134, 1226, 1236, 1413, 1422, 2169, 2711, 2758
MunI	CAATTG	1 cut(s)	2028
Mva1269I	GAATGC	2 cut(s)	1079, 2075
MvaI	CCWGG	7 cut(s)	1134, 1226, 1236, 1422, 2169, 2711, 2758
MwoI	GCNNNNNNNGC	7 cut(s)	322, 1443, 1541, 1895, 1943, 1952, 2030
NciI	CCSGG	2 cut(s)	1063, 1413
NcoI	CCATGG	1 cut(s)	583
NdeI	CATATG	1 cut(s)	2449
NlaIV	GGNNCC	8 cut(s)	193, 251, 288, 354, 670, 1782, 2305, 2608
NmeAIII	GCCGAG	1 cut(s)	132
NmuCI	GTSAC	1 cut(s)	2614
NsbI	TGCGCA	1 cut(s)	1028
NsiI	ATGCAT	1 cut(s)	550
NspI	RCATGY	1 cut(s)	2252
NspV	TTCGAA	1 cut(s)	2627
PagI	TCATGA	1 cut(s)	1309
PciSI	GCTCTTC	2 cut(s)	602, 1824
PctI	GAATGC	2 cut(s)	1079, 2075
PdmI	GAANNNNTTC	1 cut(s)	2393
PfeI	GAWTC	2 cut(s)	473, 906
PflMI	CCANNNNNTGG	1 cut(s)	2757
PfoI	TCCNGGA	2 cut(s)	1132, 2756
PkrI	GCNGC	4 cut(s)	276, 315, 1696, 2295
PleI	GAGTC	2 cut(s)	1936, 2323
PpsI	GAGTC	2 cut(s)	1936, 2323
Ppu21I	YACGTR	1 cut(s)	1155
Psp124BI	GAGCTC	2 cut(s)	390, 2026
Psp6I	CCWGG	7 cut(s)	1132, 1224, 1234, 1420, 2167, 2709, 2756
PspEI	GGTNACC	1 cut(s)	926
PspFI	CCCAGC	4 cut(s)	576, 2356, 2360, 2560
PspGI	CCWGG	7 cut(s)	1132, 1224, 1234, 1420, 2167, 2709, 2756
PspN4I	GGNNCC	8 cut(s)	193, 251, 288, 354, 670, 1782, 2305, 2608
PspPI	GGNCC	5 cut(s)	191, 250, 508, 1222, 2707
PstNI	CAGNNNCTG	1 cut(s)	1241
PsuI	RGATCY	3 cut(s)	352, 522, 688
PvuII	CAGCTG	1 cut(s)	2360
RseI	CAYNNNNRTG	4 cut(s)	755, 1022, 1554, 2016
SacI	GAGCTC	2 cut(s)	390, 2026
SapI	GCTCTTC	2 cut(s)	602, 1824
SaqAI	TTAA	5 cut(s)	1146, 1658, 2151, 2208, 2256
SatI	GCNGC	4 cut(s)	275, 314, 1695, 2294
Sau96I	GGNCC	5 cut(s)	191, 250, 508, 1222, 2707
ScaI	AGTACT	2 cut(s)	374, 2086
SchI	GAGTC	2 cut(s)	1937, 2323
ScrFI	CCNGG	9 cut(s)	1063, 1134, 1226, 1236, 1413, 1422, 2169, 2711, 2758
SduI	GDGCHC	4 cut(s)	390, 1406, 2026, 2645
SfaNI	GCATC	6 cut(s)	253, 535, 557, 1564, 2227, 2594
SfuI	TTCGAA	1 cut(s)	2627
SinI	GGWCC	3 cut(s)	250, 508, 2707
SmiMI	CAYNNNNRTG	4 cut(s)	755, 1022, 1554, 2016
SmlI	CTYRAG	4 cut(s)	376, 518, 2080, 2594
SmoI	CTYRAG	4 cut(s)	376, 518, 2080, 2594
SpeI	ACTAGT	3 cut(s)	830, 1538, 2618
SsiI	CCGC	6 cut(s)	144, 277, 405, 425, 1663, 2106
SspI	AATATT	1 cut(s)	1773
SstI	GAGCTC	2 cut(s)	390, 2026
StyD4I	CCNGG	9 cut(s)	1061, 1132, 1224, 1234, 1411, 1420, 2167, 2709, 2756
StyI	CCWWGG	3 cut(s)	583, 1904, 2530
TaiI	ACGT	4 cut(s)	1026, 1119, 1157, 1403
TaqI	TCGA	2 cut(s)	1128, 2627
TatI	WGTACW	4 cut(s)	372, 855, 2084, 2260
TfiI	GAWTC	2 cut(s)	473, 906
Tru1I	TTAA	5 cut(s)	1146, 1658, 2151, 2208, 2256
Tru9I	TTAA	5 cut(s)	1146, 1658, 2151, 2208, 2256
TscAI	CASTG	9 cut(s)	634, 661, 843, 948, 1831, 1928, 2023, 2619, 2722
TseFI	GTSAC	1 cut(s)	2614
TseI	GCWGC	4 cut(s)	274, 313, 1694, 2293
Tsp45I	GTSAC	1 cut(s)	2614
TspGWI	ACGGA	2 cut(s)	248, 309
TspRI	CASTG	9 cut(s)	634, 661, 843, 948, 1831, 1928, 2023, 2619, 2722
Van91I	CCANNNNNTGG	1 cut(s)	2757
VpaK11BI	GGWCC	3 cut(s)	250, 508, 2707
XagI	CCTNNNNNAGG	1 cut(s)	1876
XapI	RAATTY	3 cut(s)	1092, 1360, 2516
XbaI	TCTAGA	1 cut(s)	988
XceI	RCATGY	1 cut(s)	2252
XcmI	CCANNNNNNNNNTGG	2 cut(s)	891, 2701
XmiI	GTMKAC	1 cut(s)	1417
XmnI	GAANNNNTTC	1 cut(s)	2393
ZrmI	AGTACT	2 cut(s)	374, 2086
Zsp2I	ATGCAT	1 cut(s)	550

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.