MD13G1065400.v1.1

Remorin, C-terminal region

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004384
Physical Location & Seq
Forward (+)
4541155 .. 4542768
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1065400.v1.1.491

Sequence Viewer

Length: 783 bp
ATGAAGCATGCAACTGCGGTGGGAGCTGCTGCATTGGCCGTTAAATCAATCGAAGATTCGGCCATCTCTGATAAGAAAAGGACGGGCTATGAGCGCAAACCAACTTTGGAGAGGATCAAGAGCGGAAGGGAAGAAACAACAATTTCAAAGCCGGAATCTGGAAGATTTTCCAAAATATTCTCAGGTGGAGGTTCGACGAAAAGGACTCCAGAACGAGAAGATCCGCATGGCAAGGCACCAATAAGCATTGCCACAACCAGGAAGAACCCCGAAAAGGCCGTCCTTCCTGCCCCATCTATCAAGAAAACTCCAACTTTTGCTGATAAACAGTCAAACAGCGCTGGTGGTGTAAACCCTGAAACTGCAGCGCCGAAACCCAATCTGTCCACAACTAGGAAACCTGAAAGCCATTGGAATGAAACCAGGAGGCCGAGTTCAACAGGACCTGGAACAACGAAAACACAAGCAGATATTTGGGAGGAAACTGAGATCGCTAGACTCAAAGAAAGGCATGAGAAAATAAACGCCACAATACTTGCATGGGAGAAGAAGAAGAAGAAGAAAAGCACAAACCATCTCCATAAAATAGAGAGTGAAGTAGAGCGAAAAAGAGCAAAAGCACTACAAAAGTTCAGCAGCGAGATGGAAACTATCAAACAGATTGCACAAGGAGCTAGGGCAGAGGCAGAGGAGAGGCATAGGAAAGAAGTCTTGAAGGTGAAAGAAAAGGCAAATGCAATGAGAACAACAGGGAAAGCTCCTAAAACATGCTTCTGCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Remorin_C

Protein Analysis

261

Amino Acids

28.81

Weight (kDa)

10.04

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Remorin_C PF03763 153 - 255 3.1e-27 Remorin, C-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 235
AccBSI CCGCTC 1 cut(s) 123
AciI CCGC 3 cut(s) 17, 123, 224
AclWI GGATC 2 cut(s) 122, 215
AcoI YGGCCR 2 cut(s) 36, 60
AfeI AGCGCT 1 cut(s) 340
AfiI CCNNNNNNNGG 4 cut(s) 158, 258, 274, 393
AgsI TTSAA 3 cut(s) 147, 438, 715
AjnI CCWGG 3 cut(s) 257, 422, 445
AloI GAACNNNNNNTCC 2 cut(s) 418, 450
AluBI AGCT 3 cut(s) 26, 674, 758
AluI AGCT 3 cut(s) 26, 674, 758
AlwI GGATC 2 cut(s) 122, 215
AlwNI CAGNNNCTG 1 cut(s) 446
Aor51HI AGCGCT 1 cut(s) 340
AoxI GGCC 4 cut(s) 36, 60, 276, 428
ApeKI GCWGC 4 cut(s) 26, 29, 365, 636
ArsI GACNNNNNNTTYG 2 cut(s) 264, 296
Asp700I GAANNNNTTC 1 cut(s) 166
AspLEI GCGC 3 cut(s) 96, 341, 370
AspS9I GGNCC 1 cut(s) 443
AsuHPI GGTGA 1 cut(s) 730
AvaII GGWCC 1 cut(s) 443
BanI GGYRCC 1 cut(s) 235
BbvI GCAGC 4 cut(s) 13, 16, 377, 648
BccI CCATC 4 cut(s) 71, 301, 582, 637
BceAI ACGGC 2 cut(s) 23, 263
BciT130I CCWGG 3 cut(s) 259, 424, 447
BfaI CTAG 3 cut(s) 393, 495, 675
BfmI CTRYAG 1 cut(s) 363
BfoI RGCGCY 2 cut(s) 342, 371
BisI GCNGC 4 cut(s) 27, 30, 366, 637
BlsI GCNGC 4 cut(s) 28, 31, 367, 638
Bme1390I CCNGG 3 cut(s) 259, 424, 447
Bme18I GGWCC 1 cut(s) 443
BmgT120I GGNCC 1 cut(s) 443
BmiI GGNNCC 1 cut(s) 237
BmrFI CCNGG 3 cut(s) 259, 424, 447
BpmI CTGGAG 1 cut(s) 192
BsaXI ACNNNNNCTCC 2 cut(s) 418, 448
Bsc4I CCNNNNNNNGG 4 cut(s) 158, 258, 274, 393
Bse3DI GCAATG 2 cut(s) 246, 744
BseBI CCWGG 3 cut(s) 259, 424, 447
BseLI CCNNNNNNNGG 4 cut(s) 158, 258, 274, 393
BseMI GCAATG 2 cut(s) 246, 744
BseMII CTCAG 2 cut(s) 195, 477
BseRI GAGGAG 1 cut(s) 704
BseXI GCAGC 4 cut(s) 13, 16, 377, 648
BshFI GGCC 4 cut(s) 38, 62, 278, 430
BshNI GGYRCC 1 cut(s) 235
BsiSI CCGG 1 cut(s) 152
BslI CCNNNNNNNGG 4 cut(s) 158, 258, 274, 393
BsnI GGCC 4 cut(s) 38, 62, 278, 430
Bsp143I GATC 3 cut(s) 114, 220, 489
BspACI CCGC 3 cut(s) 17, 123, 224
BspANI GGCC 4 cut(s) 38, 62, 278, 430
BspCNI CTCAG 2 cut(s) 194, 478
BspLI GGNNCC 1 cut(s) 237
BspMAI CTGCAG 1 cut(s) 367
BspPI GGATC 2 cut(s) 122, 215
BspT107I GGYRCC 1 cut(s) 235
BsrBI CCGCTC 1 cut(s) 123
BsrDI GCAATG 2 cut(s) 246, 744
BssMI GATC 3 cut(s) 114, 220, 489
Bst2UI CCWGG 3 cut(s) 259, 424, 447
Bst4CI ACNGT 1 cut(s) 330
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 2 cut(s) 181, 486
BstH2I RGCGCY 2 cut(s) 342, 371
BstHHI GCGC 3 cut(s) 96, 341, 370
BstKTI GATC 3 cut(s) 117, 223, 492
BstMBI GATC 3 cut(s) 114, 220, 489
BstMWI GCNNNNNNNGC 4 cut(s) 23, 35, 93, 671
BstNI CCWGG 3 cut(s) 259, 424, 447
BstNSI RCATGY 2 cut(s) 11, 771
BstSCI CCNGG 3 cut(s) 257, 422, 445
BstSFI CTRYAG 1 cut(s) 363
BstV1I GCAGC 4 cut(s) 13, 16, 377, 648
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
BsuRI GGCC 4 cut(s) 38, 62, 278, 430
Cac8I GCNNGC 1 cut(s) 9
CaiI CAGNNNCTG 1 cut(s) 446
CfoI GCGC 3 cut(s) 96, 341, 370
Cfr13I GGNCC 1 cut(s) 443
CspCI CAANNNNNGTGG 3 cut(s) 35, 517, 552
CviAII CATG 5 cut(s) 8, 227, 512, 540, 768
DdeI CTNAG 2 cut(s) 181, 486
DpnI GATC 3 cut(s) 116, 222, 491
DpnII GATC 3 cut(s) 114, 220, 489
EaeI YGGCCR 2 cut(s) 36, 60
Eco47I GGWCC 1 cut(s) 443
Eco47III AGCGCT 1 cut(s) 340
EcoO109I RGGNCCY 1 cut(s) 443
EcoRII CCWGG 3 cut(s) 257, 422, 445
FaeI CATG 5 cut(s) 11, 230, 515, 543, 771
FaiI YATR 8 cut(s) 9, 90, 228, 513, 541, 582, 699, 769
FatI CATG 5 cut(s) 7, 226, 511, 539, 767
Fnu4HI GCNGC 4 cut(s) 27, 30, 366, 637
Fsp4HI GCNGC 4 cut(s) 27, 30, 366, 637
FspBI CTAG 3 cut(s) 393, 495, 675
GlaI GCGC 3 cut(s) 95, 340, 369
GluI GCNGC 4 cut(s) 27, 30, 366, 637
GsuI CTGGAG 1 cut(s) 192
HaeII RGCGCY 2 cut(s) 342, 371
HaeIII GGCC 4 cut(s) 38, 62, 278, 430
HapII CCGG 1 cut(s) 152
HhaI GCGC 3 cut(s) 96, 341, 370
Hin1II CATG 5 cut(s) 11, 230, 515, 543, 771
Hin6I GCGC 3 cut(s) 94, 339, 368
HinP1I GCGC 3 cut(s) 94, 339, 368
HinfI GANTC 4 cut(s) 56, 155, 205, 498
HpaII CCGG 1 cut(s) 152
HphI GGTGA 1 cut(s) 730
Hpy166II GTNNAC 2 cut(s) 352, 387
Hpy188I TCNGA 1 cut(s) 70
Hpy188III TCNNGA 5 cut(s) 118, 159, 209, 301, 712
Hpy8I GTNNAC 2 cut(s) 352, 387
Hpy99I CGWCG 1 cut(s) 199
HpyAV CCTTC 3 cut(s) 120, 293, 709
HpyCH4III ACNGT 1 cut(s) 330
HpyCH4V TGCA 6 cut(s) 11, 32, 365, 539, 665, 737
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 35, 93, 671
HpyF3I CTNAG 2 cut(s) 181, 486
Hsp92II CATG 5 cut(s) 11, 230, 515, 543, 771
HspAI GCGC 3 cut(s) 94, 339, 368
Kzo9I GATC 3 cut(s) 114, 220, 489
LmnI GCTCC 3 cut(s) 23, 671, 763
Lsp1109I GCAGC 4 cut(s) 13, 16, 377, 648
MaeI CTAG 3 cut(s) 393, 495, 675
MalI GATC 3 cut(s) 116, 222, 491
MbiI CCGCTC 1 cut(s) 123
MboI GATC 3 cut(s) 114, 220, 489
MflI RGATCY 1 cut(s) 220
MluCI AATT 1 cut(s) 141
MlyI GAGTC 2 cut(s) 199, 492
MmeI TCCRAC 1 cut(s) 335
MnlI CCTC 7 cut(s) 105, 182, 420, 472, 676, 682, 687
MroXI GAANNNNTTC 1 cut(s) 166
MseI TTAA 1 cut(s) 42
MslI CAYNNNNRTG 1 cut(s) 414
MspI CCGG 1 cut(s) 152
MspR9I CCNGG 3 cut(s) 259, 424, 447
MvaI CCWGG 3 cut(s) 259, 424, 447
MwoI GCNNNNNNNGC 4 cut(s) 23, 35, 93, 671
NdeII GATC 3 cut(s) 114, 220, 489
NlaIII CATG 5 cut(s) 11, 230, 515, 543, 771
NlaIV GGNNCC 1 cut(s) 237
NmeAIII GCCGAG 1 cut(s) 456
NspI RCATGY 2 cut(s) 11, 771
PaeI GCATGC 1 cut(s) 11
PdmI GAANNNNTTC 1 cut(s) 166
PfeI GAWTC 2 cut(s) 56, 155
PkrI GCNGC 4 cut(s) 28, 31, 367, 638
PleI GAGTC 2 cut(s) 199, 492
PpsI GAGTC 2 cut(s) 199, 492
PpuMI RGGWCCY 1 cut(s) 443
Psp5II RGGWCCY 1 cut(s) 443
Psp6I CCWGG 3 cut(s) 257, 422, 445
PspGI CCWGG 3 cut(s) 257, 422, 445
PspN4I GGNNCC 1 cut(s) 237
PspPI GGNCC 1 cut(s) 443
PspPPI RGGWCCY 1 cut(s) 443
PstI CTGCAG 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 446
PsuI RGATCY 1 cut(s) 220
RseI CAYNNNNRTG 1 cut(s) 414
SaqAI TTAA 1 cut(s) 42
SatI GCNGC 4 cut(s) 27, 30, 366, 637
Sau3AI GATC 3 cut(s) 114, 220, 489
Sau96I GGNCC 1 cut(s) 443
SchI GAGTC 2 cut(s) 199, 492
ScrFI CCNGG 3 cut(s) 259, 424, 447
SetI ASST 8 cut(s) 28, 187, 193, 403, 448, 676, 720, 760
SfcI CTRYAG 1 cut(s) 363
SinI GGWCC 1 cut(s) 443
SmiMI CAYNNNNRTG 1 cut(s) 414
SphI GCATGC 1 cut(s) 11
Sse9I AATT 1 cut(s) 141
SsiI CCGC 3 cut(s) 17, 123, 224
SspI AATATT 1 cut(s) 177
SspMI CTAG 3 cut(s) 393, 495, 675
StyD4I CCNGG 3 cut(s) 257, 422, 445
TaaI ACNGT 1 cut(s) 330
TaqI TCGA 2 cut(s) 51, 194
TasI AATT 1 cut(s) 141
TfiI GAWTC 2 cut(s) 56, 155
Tru1I TTAA 1 cut(s) 42
Tru9I TTAA 1 cut(s) 42
TseI GCWGC 4 cut(s) 26, 29, 365, 636
TspDTI ATGAA 2 cut(s) 17, 432
VpaK11BI GGWCC 1 cut(s) 443
XceI RCATGY 2 cut(s) 11, 771
XmnI GAANNNNTTC 1 cut(s) 166
XspI CTAG 3 cut(s) 393, 495, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.