Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003652

Physical Location & Seq

Genomic Coordinates Forward (+)

8678849 .. 8683600

Gene Structure 4752 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1118700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGGTCGTCGCACAGAAGGTGAAGGAAGCAGAGATCACCGAGCAGGACTCGCTTCTTCTGACGAGGAACTTGCTTCGTATTGCTATATTCAATATCAGTTACATCAGAGGCCTGTTTCCGGAGAAGTACTTCAATGATAAGTCTGTTCCTGCTTTAGAGATGAAGATCAAAAAGCTCATGCCAATGGATGCGGAATCTCGCAGGCTTATTGATTGGATGGAGAAAGGTGTATACGATGCATTGCAGAAGAAGTACCTGAAGACGCTATTGTTCTGCGTGTGCGAAACAGTTGAAGGACCAATGATTGAGGAATACACTTTTTCTTTCAGTTACTCAAATTCTGAAAGCCAGGAGGTTTCAATGAATATAAGTCGCTCTGGGAACAAGAAAAAAGAGGGTGGAACATTCAAGTGCAACTCCACAGCAGAAATAACTCCCAACCAGATGAGCTCTGCGTGTAAAATGGTCCGCACATTGGTTCAATTGATGAGAACTCTTGATAAAATGCCAGAAGAGCGAACCATATTGATGAAGCTCCTATACTACGATGATGTGACGCCAGCGGAGTATGAGCCTCCATTCTTCAGGGGCTGCTCTGAGGAAGAAGCTCGTAATGCATGGACCAAGAATCCTTTGAGAATGGAGGTTGGGAATGTAAACAGCAAGCATCTTGTATTAACGCTGAAGGTAAAAAGCATCCTTGATCCTTGTGAGGACGAAAATGATGATATTCAAGATGATGAAGTGAGCTTAGGAGCTGATTCTATGCAAAGGGATGATTATTCGGGATCTGATTCTGACAGTGAGGTTAACCAATCACAAGACGATCATTATATAGTTGCTCCAGTAGATAAGCAACAACCACAGAAAGATAACAGCACACCACAGGAAGATGACAGCATGGTTGATGAAGATAACACTCAGGATTCAGTGCAAGATGAACATCAATTGGCTCGGATAAAGGACTGGATCAGTAGCCTCCACCTTGACACTGTTGAACTTACTGATGTTCTCTCAAATTTCCCGGACATCTCAGTGGTTCTGACTGAAGAAATTATGGACAAGCTTGTCATAGAAGGTATTCTATCAAAACCTGCAGGGGACACTTACACTATCAACAGGCCGAAGAAGCCTGATTATGAGTTCACTATGGTGAAAGAAGAAACGGATGCTCAAGTACCAGTTGCTGACAAAACTTTAAAGGTTAATGATCTCATGTACACAAAGGCGCTGTATCATGCTCTCCCAATGCAGTATGTGACGGTTACAAGGCTTCAGAACAAGCTCGGTGGAGAAGCAAATCAGACTACTGTGCGCAAGTTCATCGATAAAATGGCACGAGAGGGTTTTCTTGAAGCCAAAGGCAACCGAAGGCTAGGAAAGCGTGTGATCCGTTCTAAGATCACCGAGAAAAAGCTGAATGAAGTGAAGAAAGCTCTGAACAATGATGCAATGGATGTTGACAACACTGAACCAAATAACAAGTCCAGTCATCCAGATTTCCATACAATGGGAAGCAGCATCAGGGACACATCCATATGCGGTGCCCTCCACTCCATTGGATCAGATCTCACACGCATGAAACTAAGATCGAACGGACCTCAATACAGTCCAATGAGGAGTGAGCAGACTGCTTCAAAGACAAAGGAGCATCCAAACACTCCCACAAGCAGGGCTGAGCCGGTGACTTCAAGGGAGAGCTTCGTGCCGGGAAAGGATAACGGCAGGGTAAATGGTAACACTGATTACTGTGATGATGGGGACAGAGTGATCTGCAGTGGGAGGTCTAGCCAAGACAAGCGGTCGAGGAAAACAAGCACGGTGAAGGAGCCAATTCTTCAGTACTTGAAGCGCCAGAAGTCTCAAGCCGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000785 GO:0000793 GO:0000794 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0006139 GO:0006259 GO:0006310 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0007275 GO:0008150 GO:0008152 GO:0009553 GO:0009554 GO:0009555 GO:0009556 GO:0009987 GO:0016043 GO:0019953 GO:0022402 GO:0022414 GO:0022607 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0034293 GO:0034641 GO:0035825 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043934 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044464 GO:0044703 GO:0045132 GO:0045143 GO:0046483 GO:0048229 GO:0048236 GO:0048285 GO:0048856 GO:0048869 GO:0051026 GO:0051276 GO:0051321 GO:0051704 GO:0061982 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0098813 GO:0140013 GO:1901360 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HORMA

Protein Analysis

625

Amino Acids

70.82

Weight (kDa)

5.47

Isoelectric Point (pI)

47.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HORMA	PF02301	17 - 223	1.5e-61	HORMA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003652)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67370
fragaria_vesca	FvH4_2g22740 FvH4_4g23791 FvH4_4g23791 FvH4_4g23800
malus_domestica	MD13G1118700.v1.1 MD16G1118900.v1.1
prunus_persica	Prupe.1G226200_v2.0.a1 Prupe.1G226200_v2.0.a1
pyrus_communis	pycom13g10360 pycom16g10180
rosa_chinensis	RchiOBHm_Chr4g0430291 RchiOBHm_Chr4g0430321 RchiOBHm_Chr4g0430341
rosa_laevigata	RLG00000006983 RLG00000006985 RLG00000006987
rosa_multiflora	Rmu_co8441339.1_g000001 Rmu_sc0000131.1_g000059 Rmu_sc0001089.1_g000027 Rmu_sc0002248.1_g000047 Rmu_sc0020995.1_g000013
rosa_roxburghii	Rroxscaffold_5G00372090 Rroxscaffold_5G00372110
rosa_rugosa	Rorug04G0242500 Rorug04G0242600
rosa_samantha	Rh4AG226000 Rh4AG298900 Rh4BG305500 Rh4CG240300 Rh4CG321000 Rh4CG321400
rosa_wichuraiana	Rw4G025920 Rw4G025930 Rw4G025940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1067
Acc16I	TGCGCA	1 cut(s)	1316
Acc36I	ACCTGC	1 cut(s)	1102
AccB1I	GGYRCC	1 cut(s)	1544
AccB7I	CCANNNNNTGG	1 cut(s)	1510
AccI	GTMKAC	1 cut(s)	231
AccIII	TCCGGA	1 cut(s)	118
AciI	CCGC	5 cut(s)	191, 469, 563, 1542, 1801
AclWI	GGATC	5 cut(s)	698, 796, 977, 1384, 1570
AcsI	RAATTY	2 cut(s)	337, 1018
AcuI	CTGAAG	6 cut(s)	278, 568, 704, 1068, 1259, 1823
AcyI	GRCGYC	1 cut(s)	557
AfaI	GTAC	5 cut(s)	128, 254, 1179, 1220, 1844
AfiI	CCNNNNNNNGG	4 cut(s)	118, 475, 1510, 1671
AhdI	GACNNNNNGTC	1 cut(s)	1801
AjnI	CCWGG	1 cut(s)	348
AleI	CACNNNNGTG	1 cut(s)	1151
Alw21I	GWGCWC	1 cut(s)	452
Alw26I	GTCTC	1 cut(s)	1866
AlwI	GGATC	5 cut(s)	698, 796, 977, 1384, 1570
AlwNI	CAGNNNCTG	2 cut(s)	591, 1187
Aor13HI	TCCGGA	1 cut(s)	118
AoxI	GGCC	2 cut(s)	109, 1121
ApeKI	GCWGC	2 cut(s)	591, 1518
ApoI	RAATTY	2 cut(s)	337, 1018
ArsI	GACNNNNNNTTYG	2 cut(s)	1469, 1501
Asp700I	GAANNNNTTC	2 cut(s)	128, 1080
AspLEI	GCGC	3 cut(s)	1231, 1317, 1854
AspS9I	GGNCC	4 cut(s)	296, 466, 621, 1598
AsuC2I	CCSGG	2 cut(s)	1025, 1710
AsuHPI	GGTGA	6 cut(s)	28, 31, 1165, 1396, 1696, 1834
AvaII	GGWCC	4 cut(s)	296, 466, 621, 1598
BaeGI	GKGCMC	1 cut(s)	1549
BanI	GGYRCC	1 cut(s)	1544
BanII	GRGCYC	1 cut(s)	452
BarI	GAAGNNNNNNTAC	2 cut(s)	524, 556
BauI	CACGAG	1 cut(s)	1338
BbsI	GAAGAC	1 cut(s)	266
Bbv12I	GWGCWC	1 cut(s)	452
BbvI	GCAGC	2 cut(s)	578, 1530
BccI	CCATC	2 cut(s)	211, 1751
BceAI	ACGGC	2 cut(s)	1738, 1853
BcgI	CGANNNNNNTGC	4 cut(s)	52, 86, 1306, 1340
BciT130I	CCWGG	1 cut(s)	350
BcnI	CCSGG	2 cut(s)	1025, 1710
BcoDI	GTCTC	1 cut(s)	1866
BfaI	CTAG	2 cut(s)	1376, 1788
BfmI	CTRYAG	2 cut(s)	1095, 1774
BfoI	RGCGCY	2 cut(s)	1232, 1855
BfuAI	ACCTGC	1 cut(s)	1102
BglII	AGATCT	1 cut(s)	1567
BisI	GCNGC	2 cut(s)	592, 1519
BlpI	GCTNAGC	1 cut(s)	1677
BlsI	GCNGC	2 cut(s)	593, 1520
BmcAI	AGTACT	2 cut(s)	128, 1844
Bme1390I	CCNGG	3 cut(s)	350, 1025, 1710
Bme18I	GGWCC	4 cut(s)	296, 466, 621, 1598
BmeRI	GACNNNNNGTC	1 cut(s)	1801
BmgT120I	GGNCC	4 cut(s)	296, 466, 621, 1598
BmiI	GGNNCC	2 cut(s)	1546, 1830
BmrFI	CCNGG	3 cut(s)	350, 1025, 1710
BmsI	GCATC	8 cut(s)	178, 226, 676, 705, 1159, 1438, 1530, 1660
BpiI	GAAGAC	1 cut(s)	266
BplI	GAGNNNNNCTC	2 cut(s)	32, 64
BpmI	CTGGAG	1 cut(s)	828
Bpu10I	CCTNAGC	1 cut(s)	751
Bpu1102I	GCTNAGC	1 cut(s)	1677
BpuEI	CTTGAG	2 cut(s)	1158, 1848
BpuMI	CCSGG	2 cut(s)	1025, 1710
Bsa29I	ATCGAT	1 cut(s)	1326
BsaBI	GATNNNNATC	2 cut(s)	164, 942
BsaHI	GRCGYC	1 cut(s)	557
BsaWI	WCCGGW	1 cut(s)	118
Bsc4I	CCNNNNNNNGG	4 cut(s)	118, 475, 1510, 1671
Bse118I	RCCGGY	1 cut(s)	1681
Bse1I	ACTGG	4 cut(s)	845, 971, 1181, 1488
Bse3DI	GCAATG	2 cut(s)	239, 1458
Bse8I	GATNNNNATC	2 cut(s)	164, 942
BseAI	TCCGGA	1 cut(s)	118
BseBI	CCWGG	1 cut(s)	350
BseCI	ATCGAT	1 cut(s)	1326
BseGI	GGATG	9 cut(s)	193, 222, 696, 781, 1174, 1462, 1492, 1532, 1651
BseJI	GATNNNNATC	2 cut(s)	164, 942
BseLI	CCNNNNNNNGG	4 cut(s)	118, 475, 1510, 1671
BseMI	GCAATG	2 cut(s)	239, 1458
BseMII	CTCAG	4 cut(s)	588, 935, 1047, 1668
BseNI	ACTGG	4 cut(s)	845, 971, 1181, 1488
BseRI	GAGGAG	1 cut(s)	1633
BseSI	GKGCMC	1 cut(s)	1549
BseXI	GCAGC	2 cut(s)	578, 1530
Bsh1285I	CGRYCG	1 cut(s)	1805
BshFI	GGCC	2 cut(s)	111, 1123
BshNI	GGYRCC	1 cut(s)	1544
BshVI	ATCGAT	1 cut(s)	1326
BsiEI	CGRYCG	1 cut(s)	1805
BsiHKAI	GWGCWC	1 cut(s)	452
BsiSI	CCGG	4 cut(s)	119, 1025, 1682, 1709
BslFI	GGGAC	3 cut(s)	1115, 1541, 1775
BslI	CCNNNNNNNGG	4 cut(s)	118, 475, 1510, 1671
BsmAI	GTCTC	1 cut(s)	1866
BsmFI	GGGAC	3 cut(s)	1115, 1541, 1775
BsnI	GGCC	2 cut(s)	111, 1123
Bsp1286I	GDGCHC	2 cut(s)	452, 1549
Bsp13I	TCCGGA	1 cut(s)	118
Bsp1407I	TGTACA	1 cut(s)	1218
Bsp1720I	GCTNAGC	1 cut(s)	1677
BspACI	CCGC	5 cut(s)	191, 469, 563, 1542, 1801
BspANI	GGCC	2 cut(s)	111, 1123
BspCNI	CTCAG	4 cut(s)	589, 934, 1046, 1669
BspDI	ATCGAT	1 cut(s)	1326
BspEI	TCCGGA	1 cut(s)	118
BspLI	GGNNCC	2 cut(s)	1546, 1830
BspMAI	CTGCAG	2 cut(s)	1099, 1778
BspMI	ACCTGC	1 cut(s)	1102
BspPI	GGATC	5 cut(s)	698, 796, 977, 1384, 1570
BspQI	GCTCTTC	1 cut(s)	507
BspT107I	GGYRCC	1 cut(s)	1544
BsrDI	GCAATG	2 cut(s)	239, 1458
BsrFI	RCCGGY	1 cut(s)	1681
BsrGI	TGTACA	1 cut(s)	1218
BsrI	ACTGG	4 cut(s)	845, 971, 1181, 1488
BssAI	RCCGGY	1 cut(s)	1681
BssNAI	GTATAC	1 cut(s)	232
BssNI	GRCGYC	1 cut(s)	557
BssSI	CACGAG	1 cut(s)	1338
Bst1107I	GTATAC	1 cut(s)	232
Bst2BI	CACGAG	1 cut(s)	1338
Bst2UI	CCWGG	1 cut(s)	350
Bst4CI	ACNGT	8 cut(s)	289, 803, 994, 1264, 1312, 1610, 1751, 1822
Bst6I	CTCTTC	1 cut(s)	507
BstACI	GRCGYC	1 cut(s)	557
BstAUI	TGTACA	1 cut(s)	1218
BstC8I	GCNNGC	3 cut(s)	203, 561, 665
BstDEI	CTNAG	7 cut(s)	597, 751, 921, 1033, 1398, 1586, 1677
BstF5I	GGATG	9 cut(s)	193, 222, 696, 781, 1174, 1462, 1492, 1532, 1651
BstH2I	RGCGCY	2 cut(s)	1232, 1855
BstHHI	GCGC	3 cut(s)	1231, 1317, 1854
BstMAI	GTCTC	1 cut(s)	1866
BstMCI	CGRYCG	1 cut(s)	1805
BstMWI	GCNNNNNNNGC	5 cut(s)	49, 514, 614, 1129, 1381
BstNI	CCWGG	1 cut(s)	350
BstSCI	CCNGG	3 cut(s)	348, 1023, 1708
BstSFI	CTRYAG	2 cut(s)	1095, 1774
BstSLI	GKGCMC	1 cut(s)	1549
BstV1I	GCAGC	2 cut(s)	578, 1530
BstV2I	GAAGAC	1 cut(s)	266
BstX2I	RGATCY	2 cut(s)	788, 1567
BstXI	CCANNNNNNTGG	1 cut(s)	1559
BstYI	RGATCY	2 cut(s)	788, 1567
BstZ17I	GTATAC	1 cut(s)	232
Bsu15I	ATCGAT	1 cut(s)	1326
BsuRI	GGCC	2 cut(s)	111, 1123
BsuTUI	ATCGAT	1 cut(s)	1326
BtsCI	GGATG	9 cut(s)	193, 222, 696, 781, 1174, 1462, 1492, 1532, 1651
BtsI	GCAGTG	1 cut(s)	1783
BtsIMutI	CAGTG	7 cut(s)	808, 936, 990, 1041, 1467, 1740, 1783
BveI	ACCTGC	1 cut(s)	1102
Cac8I	GCNNGC	3 cut(s)	203, 561, 665
CaiI	CAGNNNCTG	2 cut(s)	591, 1187
CfoI	GCGC	3 cut(s)	1231, 1317, 1854
Cfr10I	RCCGGY	1 cut(s)	1681
Cfr13I	GGNCC	4 cut(s)	296, 466, 621, 1598
ClaI	ATCGAT	1 cut(s)	1326
CseI	GACGC	2 cut(s)	271, 565
Csp6I	GTAC	5 cut(s)	127, 253, 1178, 1219, 1843
CspCI	CAANNNNNGTGG	2 cut(s)	1270, 1305
CviAII	CATG	6 cut(s)	178, 618, 901, 1216, 1238, 1579
CviQI	GTAC	5 cut(s)	127, 253, 1178, 1219, 1843
DdeI	CTNAG	7 cut(s)	597, 751, 921, 1033, 1398, 1586, 1677
DraI	TTTAAA	1 cut(s)	1200
DrdI	GACNNNNNNGTC	1 cut(s)	1067
DriI	GACNNNNNGTC	1 cut(s)	1801
DseDI	GACNNNNNNGTC	1 cut(s)	1067
Eam1104I	CTCTTC	1 cut(s)	507
Eam1105I	GACNNNNNGTC	1 cut(s)	1801
EarI	CTCTTC	1 cut(s)	507
Ecl136II	GAGCTC	1 cut(s)	450
Eco147I	AGGCCT	1 cut(s)	111
Eco24I	GRGCYC	1 cut(s)	452
Eco47I	GGWCC	4 cut(s)	296, 466, 621, 1598
Eco53kI	GAGCTC	1 cut(s)	450
Eco57I	CTGAAG	6 cut(s)	278, 568, 704, 1068, 1259, 1823
EcoICRI	GAGCTC	1 cut(s)	450
EcoRII	CCWGG	1 cut(s)	348
EcoT22I	ATGCAT	2 cut(s)	241, 619
EcoT38I	GRGCYC	1 cut(s)	452
FaeI	CATG	6 cut(s)	181, 621, 904, 1219, 1241, 1582
FaqI	GGGAC	3 cut(s)	1115, 1541, 1775
FatI	CATG	6 cut(s)	177, 617, 900, 1215, 1237, 1578
FauNDI	CATATG	1 cut(s)	1538
FblI	GTMKAC	1 cut(s)	231
Fnu4HI	GCNGC	2 cut(s)	592, 1519
FokI	GGATG	9 cut(s)	200, 229, 683, 788, 1181, 1469, 1479, 1519, 1638
FriOI	GRGCYC	1 cut(s)	452
Fsp4HI	GCNGC	2 cut(s)	592, 1519
FspBI	CTAG	2 cut(s)	1376, 1788
FspI	TGCGCA	1 cut(s)	1316
GlaI	GCGC	3 cut(s)	1230, 1316, 1853
GluI	GCNGC	2 cut(s)	592, 1519
GsuI	CTGGAG	1 cut(s)	828
HaeII	RGCGCY	2 cut(s)	1232, 1855
HaeIII	GGCC	2 cut(s)	111, 1123
HapII	CCGG	4 cut(s)	119, 1025, 1682, 1709
HgaI	GACGC	2 cut(s)	271, 565
HhaI	GCGC	3 cut(s)	1231, 1317, 1854
Hin1I	GRCGYC	1 cut(s)	557
Hin1II	CATG	6 cut(s)	181, 621, 904, 1219, 1241, 1582
Hin6I	GCGC	3 cut(s)	1229, 1315, 1852
HinP1I	GCGC	3 cut(s)	1229, 1315, 1852
HincII	GTYRAC	2 cut(s)	811, 1462
HindII	GTYRAC	2 cut(s)	811, 1462
HindIII	AAGCTT	1 cut(s)	1064
HinfI	GANTC	6 cut(s)	47, 194, 628, 761, 794, 926
HpaI	GTTAAC	1 cut(s)	811
HpaII	CCGG	4 cut(s)	119, 1025, 1682, 1709
HphI	GGTGA	6 cut(s)	28, 31, 1165, 1396, 1696, 1834
Hpy166II	GTNNAC	6 cut(s)	232, 658, 811, 1146, 1221, 1462
Hpy188III	TCNNGA	7 cut(s)	119, 497, 734, 786, 923, 1352, 1496
Hpy8I	GTNNAC	6 cut(s)	232, 658, 811, 1146, 1221, 1462
Hpy99I	CGWCG	1 cut(s)	11
HpyAV	CCTTC	7 cut(s)	10, 16, 287, 679, 1070, 1365, 1819
HpyCH4III	ACNGT	8 cut(s)	289, 803, 994, 1264, 1312, 1610, 1751, 1822
HpyF10VI	GCNNNNNNNGC	5 cut(s)	49, 514, 614, 1129, 1381
HpyF3I	CTNAG	7 cut(s)	597, 751, 921, 1033, 1398, 1586, 1677
Hsp92I	GRCGYC	1 cut(s)	557
Hsp92II	CATG	6 cut(s)	181, 621, 904, 1219, 1241, 1582
HspAI	GCGC	3 cut(s)	1229, 1315, 1852
Kpn2I	TCCGGA	1 cut(s)	118
KspAI	GTTAAC	1 cut(s)	811
LguI	GCTCTTC	1 cut(s)	507
LmnI	GCTCC	5 cut(s)	540, 755, 847, 1648, 1828
Lsp1109I	GCAGC	2 cut(s)	578, 1530
LweI	GCATC	8 cut(s)	178, 226, 676, 705, 1159, 1438, 1530, 1660
MaeI	CTAG	2 cut(s)	1376, 1788
MaeIII	GTNAC	7 cut(s)	98, 329, 553, 1258, 1264, 1684, 1736
MfeI	CAATTG	2 cut(s)	482, 947
MflI	RGATCY	2 cut(s)	788, 1567
MhlI	GDGCHC	2 cut(s)	452, 1549
MluCI	AATT	6 cut(s)	337, 482, 947, 1018, 1053, 1833
MlyI	GAGTC	1 cut(s)	41
Mph1103I	ATGCAT	2 cut(s)	241, 619
MroI	TCCGGA	1 cut(s)	118
MroXI	GAANNNNTTC	2 cut(s)	128, 1080
MseI	TTAA	4 cut(s)	677, 810, 1199, 1206
MslI	CAYNNNNRTG	7 cut(s)	182, 409, 527, 1034, 1151, 1537, 1577
MspA1I	CMGCKG	1 cut(s)	563
MspI	CCGG	4 cut(s)	119, 1025, 1682, 1709
MspR9I	CCNGG	3 cut(s)	350, 1025, 1710
MunI	CAATTG	2 cut(s)	482, 947
MvaI	CCWGG	1 cut(s)	350
MwoI	GCNNNNNNNGC	5 cut(s)	49, 514, 614, 1129, 1381
NciI	CCSGG	2 cut(s)	1025, 1710
NdeI	CATATG	1 cut(s)	1538
NlaIII	CATG	6 cut(s)	181, 621, 904, 1219, 1241, 1582
NlaIV	GGNNCC	2 cut(s)	1546, 1830
NmuCI	GTSAC	3 cut(s)	553, 1258, 1684
NsbI	TGCGCA	1 cut(s)	1316
NsiI	ATGCAT	2 cut(s)	241, 619
OliI	CACNNNNGTG	1 cut(s)	1151
PceI	AGGCCT	1 cut(s)	111
PciSI	GCTCTTC	1 cut(s)	507
PdmI	GAANNNNTTC	2 cut(s)	128, 1080
PfeI	GAWTC	5 cut(s)	194, 628, 761, 794, 926
PflMI	CCANNNNNTGG	1 cut(s)	1510
PfoI	TCCNGGA	1 cut(s)	1023
PkrI	GCNGC	2 cut(s)	593, 1520
PleI	GAGTC	1 cut(s)	41
PpsI	GAGTC	1 cut(s)	41
Psp124BI	GAGCTC	1 cut(s)	452
Psp6I	CCWGG	1 cut(s)	348
PspGI	CCWGG	1 cut(s)	348
PspN4I	GGNNCC	2 cut(s)	1546, 1830
PspPI	GGNCC	4 cut(s)	296, 466, 621, 1598
PstI	CTGCAG	2 cut(s)	1099, 1778
PstNI	CAGNNNCTG	2 cut(s)	591, 1187
PsuI	RGATCY	2 cut(s)	788, 1567
RsaI	GTAC	5 cut(s)	128, 254, 1179, 1220, 1844
RsaNI	GTAC	5 cut(s)	127, 253, 1178, 1219, 1843
RseI	CAYNNNNRTG	7 cut(s)	182, 409, 527, 1034, 1151, 1537, 1577
SacI	GAGCTC	1 cut(s)	452
SapI	GCTCTTC	1 cut(s)	507
SaqAI	TTAA	4 cut(s)	677, 810, 1199, 1206
SatI	GCNGC	2 cut(s)	592, 1519
Sau96I	GGNCC	4 cut(s)	296, 466, 621, 1598
SbfI	CCTGCAGG	1 cut(s)	1099
ScaI	AGTACT	2 cut(s)	128, 1844
SchI	GAGTC	1 cut(s)	41
ScrFI	CCNGG	3 cut(s)	350, 1025, 1710
SdaI	CCTGCAGG	1 cut(s)	1099
SduI	GDGCHC	2 cut(s)	452, 1549
SfaNI	GCATC	8 cut(s)	178, 226, 676, 705, 1159, 1438, 1530, 1660
SfcI	CTRYAG	2 cut(s)	1095, 1774
SinI	GGWCC	4 cut(s)	296, 466, 621, 1598
SmiMI	CAYNNNNRTG	7 cut(s)	182, 409, 527, 1034, 1151, 1537, 1577
SmlI	CTYRAG	2 cut(s)	1173, 1863
SmoI	CTYRAG	2 cut(s)	1173, 1863
Sse8387I	CCTGCAGG	1 cut(s)	1099
Sse9I	AATT	6 cut(s)	337, 482, 947, 1018, 1053, 1833
SseBI	AGGCCT	1 cut(s)	111
SsiI	CCGC	5 cut(s)	191, 469, 563, 1542, 1801
SspMI	CTAG	2 cut(s)	1376, 1788
SstI	GAGCTC	1 cut(s)	452
StuI	AGGCCT	1 cut(s)	111
StyD4I	CCNGG	3 cut(s)	348, 1023, 1708
TaaI	ACNGT	8 cut(s)	289, 803, 994, 1264, 1312, 1610, 1751, 1822
TaqI	TCGA	3 cut(s)	1326, 1592, 1805
TasI	AATT	6 cut(s)	337, 482, 947, 1018, 1053, 1833
TatI	WGTACW	3 cut(s)	126, 1218, 1842
TfiI	GAWTC	5 cut(s)	194, 628, 761, 794, 926
Tru1I	TTAA	4 cut(s)	677, 810, 1199, 1206
Tru9I	TTAA	4 cut(s)	677, 810, 1199, 1206
TscAI	CASTG	7 cut(s)	808, 936, 997, 1041, 1474, 1747, 1783
TseFI	GTSAC	3 cut(s)	553, 1258, 1684
TseI	GCWGC	2 cut(s)	591, 1518
Tsp45I	GTSAC	3 cut(s)	553, 1258, 1684
TspDTI	ATGAA	9 cut(s)	176, 377, 545, 756, 924, 954, 1312, 1437, 1595
TspGWI	ACGGA	3 cut(s)	1181, 1382, 1611
TspRI	CASTG	7 cut(s)	808, 936, 997, 1041, 1474, 1747, 1783
Van91I	CCANNNNNTGG	1 cut(s)	1510
VpaK11BI	GGWCC	4 cut(s)	296, 466, 621, 1598
XapI	RAATTY	2 cut(s)	337, 1018
XmiI	GTMKAC	1 cut(s)	231
XmnI	GAANNNNTTC	2 cut(s)	128, 1080
XspI	CTAG	2 cut(s)	1376, 1788
ZrmI	AGTACT	2 cut(s)	128, 1844
Zsp2I	ATGCAT	2 cut(s)	241, 619

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.