MD13G1140700.v1.1

rRNA-processing protein fcf2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006753
Physical Location & Seq
Forward (+)
10848616 .. 10851227
2612 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1140700.v1.1.491

Sequence Viewer

Length: 585 bp
ATGGCGGAAAGCAATGCGGTGATTGGGCTCTCGTGGGAGCCAAAGTTGCCTGCTTTTTCATCGGCGTCCAAAAATGGAGGTGGGTCTGGCTCCAAACCTCGCCCCGAAGGCAATCCGCTTTGGAAACCCAGCACGCAGCTTGTCGATGGCCTCTTCGTTCCGCCGAATGACCCGGTAAAGCGGAACAAACTGGCCAAGAAGCAGATCAAAGACACTGCTGGAACAAGCTGGTTTGACATGCCTGCACCAACCATGACCCCGGAGTTGCAAAAAGATCTCCAATTACTAAAGTTAAGGAATGTTATGGATCCAAAGAGACACTACAAGAAGGGTGATGCACAACCTAACAAGTATTTCCAGGTAGGGACAATAATAGAGTCCCCATTAGATTTCTTCTCGGGCAGACTTACAAAGAAGGAAAGGAAGGTGTCCCTTGCAGAGGAAGTGCTTTCTGATCGTAACCTTGGGAACTACAGAAAGCGGAAGGTTCGCGAGATAGAAGAGAAAAACCGGCCAGGCGGAAATGAAAAATGGAAGATTAAGGGGAAAGGGACGTATCAGCGTGCAAAGCAAAGAAGGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fcf2

Protein Analysis

195

Amino Acids

21.98

Weight (kDa)

10.16

Isoelectric Point (pI)

52.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fcf2 PF08698 70 - 162 1.8e-36 Fcf2 pre-rRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 492
AciI CCGC 7 cut(s) 5, 17, 116, 161, 181, 481, 519
AclWI GGATC 2 cut(s) 302, 315
AcoI YGGCCR 2 cut(s) 192, 512
AcyI GRCGYC 1 cut(s) 65
AfiI CCNNNNNNNGG 1 cut(s) 439
AjnI CCWGG 2 cut(s) 357, 514
AluBI AGCT 2 cut(s) 139, 228
AluI AGCT 2 cut(s) 139, 228
Alw26I GTCTC 1 cut(s) 310
AlwI GGATC 2 cut(s) 302, 315
Ama87I CYCGRG 1 cut(s) 397
AoxI GGCC 3 cut(s) 148, 192, 512
ApeKI GCWGC 1 cut(s) 136
AsuC2I CCSGG 2 cut(s) 173, 260
AsuHPI GGTGA 2 cut(s) 31, 344
AvaI CYCGRG 1 cut(s) 397
BalI TGGCCA 1 cut(s) 194
BamHI GGATCC 1 cut(s) 307
BanII GRGCYC 1 cut(s) 30
BauI CACGAG 1 cut(s) 31
BbvI GCAGC 1 cut(s) 148
BccI CCATC 1 cut(s) 140
BciT130I CCWGG 2 cut(s) 359, 516
BcnI CCSGG 2 cut(s) 173, 260
BcoDI GTCTC 1 cut(s) 310
BfmI CTRYAG 1 cut(s) 472
BglI GCCNNNNNGGC 1 cut(s) 108
BglII AGATCT 1 cut(s) 274
BisI GCNGC 1 cut(s) 137
BlsI GCNGC 1 cut(s) 138
Bme1390I CCNGG 4 cut(s) 173, 260, 359, 516
BmeT110I CYCGRG 1 cut(s) 397
BmiI GGNNCC 3 cut(s) 39, 91, 309
BmrFI CCNGG 4 cut(s) 173, 260, 359, 516
BmsI GCATC 1 cut(s) 325
BpuMI CCSGG 2 cut(s) 173, 260
BsaHI GRCGYC 1 cut(s) 65
BsaJI CCNNGG 2 cut(s) 258, 463
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse118I RCCGGY 1 cut(s) 510
Bse1I ACTGG 1 cut(s) 195
Bse3DI GCAATG 1 cut(s) 19
BseBI CCWGG 2 cut(s) 359, 516
BseDI CCNNGG 2 cut(s) 258, 463
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMI GCAATG 1 cut(s) 19
BseNI ACTGG 1 cut(s) 195
BseXI GCAGC 1 cut(s) 148
BseYI CCCAGC 1 cut(s) 128
BsgI GTGCAG 1 cut(s) 228
Bsh1236I CGCG 1 cut(s) 492
BshFI GGCC 3 cut(s) 150, 194, 514
BsiHKCI CYCGRG 1 cut(s) 397
BsiSI CCGG 3 cut(s) 173, 260, 511
BslFI GGGAC 4 cut(s) 364, 379, 415, 565
BslI CCNNNNNNNGG 1 cut(s) 439
BsmAI GTCTC 1 cut(s) 310
BsmFI GGGAC 4 cut(s) 364, 379, 415, 565
BsnI GGCC 3 cut(s) 150, 194, 514
BsoBI CYCGRG 1 cut(s) 397
Bsp1286I GDGCHC 1 cut(s) 30
Bsp143I GATC 4 cut(s) 204, 274, 307, 454
Bsp68I TCGCGA 1 cut(s) 492
BspACI CCGC 7 cut(s) 5, 17, 116, 161, 181, 481, 519
BspANI GGCC 3 cut(s) 150, 194, 514
BspFNI CGCG 1 cut(s) 492
BspLI GGNNCC 3 cut(s) 39, 91, 309
BspPI GGATC 2 cut(s) 302, 315
BsrDI GCAATG 1 cut(s) 19
BsrFI RCCGGY 1 cut(s) 510
BsrI ACTGG 1 cut(s) 195
BssAI RCCGGY 1 cut(s) 510
BssECI CCNNGG 2 cut(s) 258, 463
BssMI GATC 4 cut(s) 204, 274, 307, 454
BssNI GRCGYC 1 cut(s) 65
BssSI CACGAG 1 cut(s) 31
BssT1I CCWWGG 1 cut(s) 463
Bst2BI CACGAG 1 cut(s) 31
Bst2UI CCWGG 2 cut(s) 359, 516
Bst6I CTCTTC 2 cut(s) 158, 495
BstACI GRCGYC 1 cut(s) 65
BstC8I GCNNGC 4 cut(s) 51, 134, 243, 564
BstENI CCTNNNNNAGG 1 cut(s) 437
BstFNI CGCG 1 cut(s) 492
BstKTI GATC 4 cut(s) 207, 277, 310, 457
BstMAI GTCTC 1 cut(s) 310
BstMBI GATC 4 cut(s) 204, 274, 307, 454
BstMWI GCNNNNNNNGC 4 cut(s) 46, 108, 568, 577
BstNI CCWGG 2 cut(s) 359, 516
BstNSI RCATGY 1 cut(s) 241
BstSCI CCNGG 4 cut(s) 171, 258, 357, 514
BstSFI CTRYAG 1 cut(s) 472
BstUI CGCG 1 cut(s) 492
BstV1I GCAGC 1 cut(s) 148
BstX2I RGATCY 2 cut(s) 274, 307
BstYI RGATCY 2 cut(s) 274, 307
BsuRI GGCC 3 cut(s) 150, 194, 514
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 2 cut(s) 213, 580
BtuMI TCGCGA 1 cut(s) 492
Cac8I GCNNGC 4 cut(s) 51, 134, 243, 564
Cfr10I RCCGGY 1 cut(s) 510
CseI GACGC 1 cut(s) 54
CviAII CATG 2 cut(s) 238, 253
CviJI RGCY 8 cut(s) 28, 40, 90, 139, 150, 194, 228, 514
CviKI_1 RGCY 8 cut(s) 28, 40, 90, 139, 150, 194, 228, 514
DpnI GATC 4 cut(s) 206, 276, 309, 456
DpnII GATC 4 cut(s) 204, 274, 307, 454
EaeI YGGCCR 2 cut(s) 192, 512
Eam1104I CTCTTC 2 cut(s) 158, 495
EarI CTCTTC 2 cut(s) 158, 495
EciI GGCGGA 3 cut(s) 20, 150, 534
Eco130I CCWWGG 1 cut(s) 463
Eco24I GRGCYC 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 397
EcoNI CCTNNNNNAGG 1 cut(s) 437
EcoRII CCWGG 2 cut(s) 357, 514
EcoT14I CCWWGG 1 cut(s) 463
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 463
FaeI CATG 2 cut(s) 241, 256
FaiI YATR 3 cut(s) 239, 254, 305
FaqI GGGAC 4 cut(s) 364, 379, 415, 565
FatI CATG 2 cut(s) 237, 252
Fnu4HI GCNGC 1 cut(s) 137
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 1 cut(s) 137
GluI GCNGC 1 cut(s) 137
GsaI CCCAGC 1 cut(s) 132
HaeIII GGCC 3 cut(s) 150, 194, 514
HapII CCGG 3 cut(s) 173, 260, 511
HgaI GACGC 1 cut(s) 54
Hin1I GRCGYC 1 cut(s) 65
Hin1II CATG 2 cut(s) 241, 256
HinfI GANTC 1 cut(s) 377
HpaII CCGG 3 cut(s) 173, 260, 511
HphI GGTGA 2 cut(s) 31, 344
Hpy188I TCNGA 1 cut(s) 454
Hpy188III TCNNGA 1 cut(s) 491
HpyAV CCTTC 6 cut(s) 101, 322, 409, 418, 478, 570
HpyCH4IV ACGT 1 cut(s) 554
HpyCH4V TGCA 5 cut(s) 245, 268, 338, 437, 566
HpyF10VI GCNNNNNNNGC 4 cut(s) 46, 108, 568, 577
HpySE526I ACGT 1 cut(s) 554
Hsp92I GRCGYC 1 cut(s) 65
Hsp92II CATG 2 cut(s) 241, 256
Kzo9I GATC 4 cut(s) 204, 274, 307, 454
LmnI GCTCC 2 cut(s) 37, 95
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 1 cut(s) 325
MaeII ACGT 1 cut(s) 554
MaeIII GTNAC 1 cut(s) 458
MalI GATC 4 cut(s) 206, 276, 309, 456
MboI GATC 4 cut(s) 204, 274, 307, 454
MboII GAAGA 4 cut(s) 145, 385, 512, 547
MflI RGATCY 2 cut(s) 274, 307
MhlI GDGCHC 1 cut(s) 30
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 1 cut(s) 281
MluNI TGGCCA 1 cut(s) 194
MlyI GAGTC 1 cut(s) 386
MnlI CCTC 4 cut(s) 71, 108, 161, 433
Mox20I TGGCCA 1 cut(s) 194
MscI TGGCCA 1 cut(s) 194
MseI TTAA 2 cut(s) 293, 540
Msp20I TGGCCA 1 cut(s) 194
MspI CCGG 3 cut(s) 173, 260, 511
MspR9I CCNGG 4 cut(s) 173, 260, 359, 516
MvaI CCWGG 2 cut(s) 359, 516
MvnI CGCG 1 cut(s) 492
MwoI GCNNNNNNNGC 4 cut(s) 46, 108, 568, 577
NciI CCSGG 2 cut(s) 173, 260
NdeII GATC 4 cut(s) 204, 274, 307, 454
NlaIII CATG 2 cut(s) 241, 256
NlaIV GGNNCC 3 cut(s) 39, 91, 309
NruI TCGCGA 1 cut(s) 492
NspI RCATGY 1 cut(s) 241
PkrI GCNGC 1 cut(s) 138
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
Psp6I CCWGG 2 cut(s) 357, 514
PspFI CCCAGC 1 cut(s) 128
PspGI CCWGG 2 cut(s) 357, 514
PspN4I GGNNCC 3 cut(s) 39, 91, 309
PsuI RGATCY 2 cut(s) 274, 307
RruI TCGCGA 1 cut(s) 492
SaqAI TTAA 2 cut(s) 293, 540
SatI GCNGC 1 cut(s) 137
Sau3AI GATC 4 cut(s) 204, 274, 307, 454
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 4 cut(s) 173, 260, 359, 516
SduI GDGCHC 1 cut(s) 30
SfaNI GCATC 1 cut(s) 325
SfcI CTRYAG 1 cut(s) 472
Sse9I AATT 1 cut(s) 281
SsiI CCGC 7 cut(s) 5, 17, 116, 161, 181, 481, 519
StyD4I CCNGG 4 cut(s) 171, 258, 357, 514
StyI CCWWGG 1 cut(s) 463
TaiI ACGT 1 cut(s) 557
TaqI TCGA 1 cut(s) 144
TasI AATT 1 cut(s) 281
Tru1I TTAA 2 cut(s) 293, 540
Tru9I TTAA 2 cut(s) 293, 540
TscAI CASTG 1 cut(s) 220
TseI GCWGC 1 cut(s) 136
TspDTI ATGAA 2 cut(s) 48, 540
TspRI CASTG 1 cut(s) 220
XagI CCTNNNNNAGG 1 cut(s) 437
XceI RCATGY 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.