Rw4G023770

rRNA-processing protein fcf2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0006753
Physical Location & Seq
Reverse (-)
49542503 .. 49544618
2116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G023770.1

Sequence Viewer

Length: 582 bp
ATGGCGGATATCAAAGCAGTAGTTGGTCTCTCATGGGAGCCAAAGATTCCGAATTTTTCATCGGCAACCAAACCTAGACTTGGAGTTGATGCAAAACCTCAAGAAACCAATCATCTTTGGAAACCCCCCACCCAGCTTGTGGATGGCCTCTTTGTGCCACCCAATAACCCCAAAACGCTGAACAAGTTGCAGAGGAAGCAACTCAAAGACACGACTGGCTCGAATTGGTTTGACATGCCTGCACCAACCATGACCCCGGAGTTGCAGAAAGATCTCCAATTACTCAAGTTAAGGAGTGTCATGGATCCAAAGAGACACTACAAGAAGGGTGATTCGCAAACTTCCAAGTATTTCCAGGTGGGAACGGTGGTAGAGTCCTCATCAGAATTCTTCTCAGGTAGACTAACAAAGAAGGAAAGGAAGGCAACCGTTGCAGAGGAGCTGCTTTCTGATCCTGCCCTTGCGACCTACAGGAAGCGGAAGGTTCGTGAGATCGAAGAGAAGAATCGACCGGCTGATAATGAAAAATGGAAGAATAAAGGGAAGACGTCCTATAAGCGTGCAAAGCAAAGAAGGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fcf2

Protein Analysis

193

Amino Acids

22.2

Weight (kDa)

10.08

Isoelectric Point (pI)

50.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fcf2 PF08698 69 - 161 1.7e-37 Fcf2 pre-rRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 551
AccB7I CCANNNNNTGG 1 cut(s) 139
AccI GTMKAC 1 cut(s) 400
AciI CCGC 2 cut(s) 5, 478
AclWI GGATC 3 cut(s) 299, 312, 446
AcsI RAATTY 2 cut(s) 52, 386
AcyI GRCGYC 1 cut(s) 548
AfiI CCNNNNNNNGG 2 cut(s) 80, 139
AjnI CCWGG 1 cut(s) 354
AluBI AGCT 2 cut(s) 136, 442
AluI AGCT 2 cut(s) 136, 442
Alw26I GTCTC 2 cut(s) 32, 307
AlwI GGATC 3 cut(s) 299, 312, 446
AoxI GGCC 1 cut(s) 145
ApeKI GCWGC 1 cut(s) 442
ApoI RAATTY 2 cut(s) 52, 386
AsuC2I CCSGG 1 cut(s) 257
AsuHPI GGTGA 1 cut(s) 341
BamHI GGATCC 1 cut(s) 304
BbsI GAAGAC 1 cut(s) 551
BbvI GCAGC 1 cut(s) 429
BccI CCATC 1 cut(s) 137
BciT130I CCWGG 1 cut(s) 356
BcnI CCSGG 1 cut(s) 257
BcoDI GTCTC 2 cut(s) 32, 307
BfaI CTAG 1 cut(s) 75
BfmI CTRYAG 1 cut(s) 469
BglII AGATCT 1 cut(s) 271
BisI GCNGC 1 cut(s) 443
BlsI GCNGC 1 cut(s) 444
Bme1390I CCNGG 2 cut(s) 257, 356
BmiI GGNNCC 2 cut(s) 39, 306
BmrFI CCNGG 2 cut(s) 257, 356
BmsI GCATC 1 cut(s) 79
BpiI GAAGAC 1 cut(s) 551
BpuEI CTTGAG 2 cut(s) 84, 269
BpuMI CCSGG 1 cut(s) 257
BsaHI GRCGYC 1 cut(s) 548
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 255
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 139
Bse118I RCCGGY 1 cut(s) 511
Bse1I ACTGG 1 cut(s) 220
BseBI CCWGG 1 cut(s) 356
BseDI CCNNGG 1 cut(s) 255
BseGI GGATG 1 cut(s) 148
BseLI CCNNNNNNNGG 2 cut(s) 80, 139
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 1 cut(s) 220
BseRI GAGGAG 1 cut(s) 452
BseXI GCAGC 1 cut(s) 429
BseYI CCCAGC 1 cut(s) 132
BsgI GTGCAG 1 cut(s) 225
Bsh1285I CGRYCG 1 cut(s) 512
BshFI GGCC 1 cut(s) 147
BsiEI CGRYCG 1 cut(s) 512
BsiSI CCGG 2 cut(s) 257, 512
BslI CCNNNNNNNGG 2 cut(s) 80, 139
BsmAI GTCTC 2 cut(s) 32, 307
BsnI GGCC 1 cut(s) 147
Bso31I GGTCTC 1 cut(s) 32
Bsp143I GATC 4 cut(s) 271, 304, 451, 492
BspACI CCGC 2 cut(s) 5, 478
BspANI GGCC 1 cut(s) 147
BspCNI CTCAG 1 cut(s) 407
BspLI GGNNCC 2 cut(s) 39, 306
BspPI GGATC 3 cut(s) 299, 312, 446
BspTNI GGTCTC 1 cut(s) 32
BsrFI RCCGGY 1 cut(s) 511
BsrI ACTGG 1 cut(s) 220
BssAI RCCGGY 1 cut(s) 511
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 4 cut(s) 271, 304, 451, 492
BssNI GRCGYC 1 cut(s) 548
Bst2UI CCWGG 1 cut(s) 356
Bst4CI ACNGT 2 cut(s) 367, 430
Bst6I CTCTTC 1 cut(s) 492
BstACI GRCGYC 1 cut(s) 548
BstAPI GCANNNNNTGC 1 cut(s) 431
BstC8I GCNNGC 2 cut(s) 240, 561
BstDEI CTNAG 1 cut(s) 394
BstF5I GGATG 1 cut(s) 148
BstKTI GATC 4 cut(s) 274, 307, 454, 495
BstMAI GTCTC 2 cut(s) 32, 307
BstMBI GATC 4 cut(s) 271, 304, 451, 492
BstMCI CGRYCG 1 cut(s) 512
BstMWI GCNNNNNNNGC 4 cut(s) 196, 431, 565, 574
BstNI CCWGG 1 cut(s) 356
BstNSI RCATGY 1 cut(s) 238
BstSCI CCNGG 2 cut(s) 255, 354
BstSFI CTRYAG 1 cut(s) 469
BstV1I GCAGC 1 cut(s) 429
BstV2I GAAGAC 1 cut(s) 551
BstX2I RGATCY 2 cut(s) 271, 304
BstYI RGATCY 2 cut(s) 271, 304
BsuRI GGCC 1 cut(s) 147
BtsCI GGATG 1 cut(s) 148
Cac8I GCNNGC 2 cut(s) 240, 561
Cfr10I RCCGGY 1 cut(s) 511
CspCI CAANNNNNGTGG 2 cut(s) 118, 153
CviAII CATG 4 cut(s) 33, 235, 250, 301
CviJI RGCY 6 cut(s) 40, 136, 147, 219, 442, 515
CviKI_1 RGCY 6 cut(s) 40, 136, 147, 219, 442, 515
DdeI CTNAG 1 cut(s) 394
DpnI GATC 4 cut(s) 273, 306, 453, 494
DpnII GATC 4 cut(s) 271, 304, 451, 492
Eam1104I CTCTTC 1 cut(s) 492
EarI CTCTTC 1 cut(s) 492
EciI GGCGGA 1 cut(s) 20
Eco31I GGTCTC 1 cut(s) 32
Eco32I GATATC 1 cut(s) 10
EcoRI GAATTC 1 cut(s) 386
EcoRII CCWGG 1 cut(s) 354
EcoRV GATATC 1 cut(s) 10
FaeI CATG 4 cut(s) 36, 238, 253, 304
FaiI YATR 5 cut(s) 34, 236, 251, 302, 555
FatI CATG 4 cut(s) 32, 234, 249, 300
FblI GTMKAC 1 cut(s) 400
Fnu4HI GCNGC 1 cut(s) 443
FokI GGATG 1 cut(s) 155
Fsp4HI GCNGC 1 cut(s) 443
FspBI CTAG 1 cut(s) 75
GluI GCNGC 1 cut(s) 443
GsaI CCCAGC 1 cut(s) 136
HaeIII GGCC 1 cut(s) 147
HapII CCGG 2 cut(s) 257, 512
Hin1I GRCGYC 1 cut(s) 548
Hin1II CATG 4 cut(s) 36, 238, 253, 304
HinfI GANTC 4 cut(s) 46, 332, 374, 505
HpaII CCGG 2 cut(s) 257, 512
HphI GGTGA 1 cut(s) 341
Hpy166II GTNNAC 1 cut(s) 401
Hpy188I TCNGA 3 cut(s) 51, 385, 451
Hpy188III TCNNGA 2 cut(s) 101, 488
Hpy8I GTNNAC 1 cut(s) 401
HpyAV CCTTC 5 cut(s) 319, 406, 415, 475, 567
HpyCH4III ACNGT 2 cut(s) 367, 430
HpyCH4IV ACGT 1 cut(s) 548
HpyCH4V TGCA 6 cut(s) 92, 190, 242, 265, 434, 563
HpyF10VI GCNNNNNNNGC 4 cut(s) 196, 431, 565, 574
HpyF3I CTNAG 1 cut(s) 394
HpySE526I ACGT 1 cut(s) 548
Hsp92I GRCGYC 1 cut(s) 548
Hsp92II CATG 4 cut(s) 36, 238, 253, 304
Kzo9I GATC 4 cut(s) 271, 304, 451, 492
LmnI GCTCC 2 cut(s) 37, 439
Lsp1109I GCAGC 1 cut(s) 429
LweI GCATC 1 cut(s) 79
MaeI CTAG 1 cut(s) 75
MaeII ACGT 1 cut(s) 548
MalI GATC 4 cut(s) 273, 306, 453, 494
MboI GATC 4 cut(s) 271, 304, 451, 492
MboII GAAGA 5 cut(s) 382, 509, 514, 544, 556
MflI RGATCY 2 cut(s) 271, 304
MluCI AATT 4 cut(s) 52, 223, 278, 386
MlyI GAGTC 1 cut(s) 383
MnlI CCTC 5 cut(s) 108, 158, 186, 388, 430
MseI TTAA 1 cut(s) 290
MspI CCGG 2 cut(s) 257, 512
MspR9I CCNGG 2 cut(s) 257, 356
MvaI CCWGG 1 cut(s) 356
MwoI GCNNNNNNNGC 4 cut(s) 196, 431, 565, 574
NciI CCSGG 1 cut(s) 257
NdeII GATC 4 cut(s) 271, 304, 451, 492
NlaIII CATG 4 cut(s) 36, 238, 253, 304
NlaIV GGNNCC 2 cut(s) 39, 306
NspI RCATGY 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 218
PfeI GAWTC 3 cut(s) 46, 332, 505
PflMI CCANNNNNTGG 1 cut(s) 139
PkrI GCNGC 1 cut(s) 444
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 132
PspGI CCWGG 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 39, 306
PsuI RGATCY 2 cut(s) 271, 304
SaqAI TTAA 1 cut(s) 290
SatI GCNGC 1 cut(s) 443
Sau3AI GATC 4 cut(s) 271, 304, 451, 492
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 2 cut(s) 257, 356
SetI ASST 9 cut(s) 76, 100, 138, 360, 400, 444, 470, 486, 551
SfaNI GCATC 1 cut(s) 79
SfcI CTRYAG 1 cut(s) 469
SmlI CTYRAG 2 cut(s) 99, 284
SmoI CTYRAG 2 cut(s) 99, 284
Sse9I AATT 4 cut(s) 52, 223, 278, 386
SsiI CCGC 2 cut(s) 5, 478
SspMI CTAG 1 cut(s) 75
StyD4I CCNGG 2 cut(s) 255, 354
TaaI ACNGT 2 cut(s) 367, 430
TaiI ACGT 1 cut(s) 551
TaqI TCGA 3 cut(s) 221, 495, 508
TasI AATT 4 cut(s) 52, 223, 278, 386
TfiI GAWTC 3 cut(s) 46, 332, 505
Tru1I TTAA 1 cut(s) 290
Tru9I TTAA 1 cut(s) 290
TseI GCWGC 1 cut(s) 442
TspDTI ATGAA 2 cut(s) 48, 537
Van91I CCANNNNNTGG 1 cut(s) 139
XapI RAATTY 2 cut(s) 52, 386
XceI RCATGY 1 cut(s) 238
XcmI CCANNNNNNNNNTGG 2 cut(s) 136, 140
XmiI GTMKAC 1 cut(s) 400
XspI CTAG 1 cut(s) 75
ZraI GACGTC 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.