MD16G1136700.v1.1

rRNA-processing protein fcf2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006753
Physical Location & Seq
Forward (+)
10452856 .. 10454815
1960 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1136700.v1.1.491

Sequence Viewer

Length: 567 bp
ATGGCGGAAAGCAAGGCGGCGATTGGGCTCTCATGGGAGCCAAAGTTGCCGGCATTTTCATCGGGGTCTGGCTCCAAACCTCGCCCCGAAGCCAATCCGCTTTGGAAACCCAGTACGCAGCTTGTCGATGGCCTCTTCGTTCCGCCGAATGACCCGGTAAAGAGGAACAAATTGGCCAAGAAGCAGATCAAAGACACAGCTGGAACAAGCTGGTTTGACATGCCTGCACCAACCATGACCCCGGAGTTGCAGAAAGATCTCCAATTGCTCAAGTTAAGGAATGTTATGGATCCAAAGAGACATTACAAAAAGGGGGATGCACAACCGAACAAGTATTTCCAGGTAGGGACAGTGATAGAGTCCCCATTAGATTTCTTCTCAAGCAGACTGACAAAGAAGGAAAGGAAGGTGTCCCTTGCAGAGGAAGTGCTTTCTGATCGTAACCTTGGGAACTACAGAAAGCGGAAGGTTCGCGAGATAGAAGAGAAAAACCGGCCTGGTGGAAATGAAAAATGGAAGATAAAAGGGAAAGGGTCGTATCAGCGTGCAAAGCAAAGAAGGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fcf2

Protein Analysis

189

Amino Acids

21.47

Weight (kDa)

10.16

Isoelectric Point (pI)

56.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fcf2 PF08698 64 - 156 1.2e-36 Fcf2 pre-rRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 474
AciI CCGC 5 cut(s) 5, 17, 98, 143, 463
AclWI GGATC 2 cut(s) 284, 297
AcoI YGGCCR 1 cut(s) 174
AfaI GTAC 1 cut(s) 115
AfiI CCNNNNNNNGG 1 cut(s) 421
AjnI CCWGG 2 cut(s) 339, 496
AluBI AGCT 3 cut(s) 121, 200, 210
AluI AGCT 3 cut(s) 121, 200, 210
Alw26I GTCTC 1 cut(s) 292
AlwI GGATC 2 cut(s) 284, 297
AoxI GGCC 3 cut(s) 130, 174, 494
ApeKI GCWGC 1 cut(s) 118
AsuC2I CCSGG 2 cut(s) 155, 242
BalI TGGCCA 1 cut(s) 176
BamHI GGATCC 1 cut(s) 289
BanII GRGCYC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 130
BccI CCATC 1 cut(s) 122
BcgI CGANNNNNNTGC 2 cut(s) 42, 76
BciT130I CCWGG 2 cut(s) 341, 498
BcnI CCSGG 2 cut(s) 155, 242
BcoDI GTCTC 1 cut(s) 292
BfmI CTRYAG 1 cut(s) 454
BglII AGATCT 1 cut(s) 256
BisI GCNGC 2 cut(s) 18, 119
BlsI GCNGC 2 cut(s) 19, 120
Bme1390I CCNGG 4 cut(s) 155, 242, 341, 498
BmiI GGNNCC 3 cut(s) 39, 73, 291
BmrFI CCNGG 4 cut(s) 155, 242, 341, 498
BmrI ACTGGG 1 cut(s) 105
BmsI GCATC 1 cut(s) 307
BmuI ACTGGG 1 cut(s) 105
BpuEI CTTGAG 2 cut(s) 254, 364
BpuMI CCSGG 2 cut(s) 155, 242
BsaJI CCNNGG 2 cut(s) 240, 445
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 1 cut(s) 421
Bse118I RCCGGY 2 cut(s) 49, 492
Bse1I ACTGG 1 cut(s) 111
BseBI CCWGG 2 cut(s) 341, 498
BseDI CCNNGG 2 cut(s) 240, 445
BseGI GGATG 1 cut(s) 322
BseLI CCNNNNNNNGG 1 cut(s) 421
BseNI ACTGG 1 cut(s) 111
BseXI GCAGC 1 cut(s) 130
BsgI GTGCAG 1 cut(s) 210
Bsh1236I CGCG 1 cut(s) 474
BshFI GGCC 3 cut(s) 132, 176, 496
BsiSI CCGG 4 cut(s) 50, 155, 242, 493
BslFI GGGAC 3 cut(s) 346, 361, 397
BslI CCNNNNNNNGG 1 cut(s) 421
BsmAI GTCTC 1 cut(s) 292
BsmFI GGGAC 3 cut(s) 346, 361, 397
BsnI GGCC 3 cut(s) 132, 176, 496
Bsp1286I GDGCHC 1 cut(s) 30
Bsp143I GATC 4 cut(s) 186, 256, 289, 436
Bsp68I TCGCGA 1 cut(s) 474
BspACI CCGC 5 cut(s) 5, 17, 98, 143, 463
BspANI GGCC 3 cut(s) 132, 176, 496
BspFNI CGCG 1 cut(s) 474
BspLI GGNNCC 3 cut(s) 39, 73, 291
BspPI GGATC 2 cut(s) 284, 297
BsrFI RCCGGY 2 cut(s) 49, 492
BsrI ACTGG 1 cut(s) 111
BssAI RCCGGY 2 cut(s) 49, 492
BssECI CCNNGG 2 cut(s) 240, 445
BssMI GATC 4 cut(s) 186, 256, 289, 436
BssT1I CCWWGG 1 cut(s) 445
Bst2UI CCWGG 2 cut(s) 341, 498
Bst4CI ACNGT 1 cut(s) 352
Bst6I CTCTTC 2 cut(s) 140, 477
BstC8I GCNNGC 3 cut(s) 51, 225, 546
BstENI CCTNNNNNAGG 1 cut(s) 419
BstF5I GGATG 1 cut(s) 322
BstFNI CGCG 1 cut(s) 474
BstKTI GATC 4 cut(s) 189, 259, 292, 439
BstMAI GTCTC 1 cut(s) 292
BstMBI GATC 4 cut(s) 186, 256, 289, 436
BstMWI GCNNNNNNNGC 3 cut(s) 46, 550, 559
BstNI CCWGG 2 cut(s) 341, 498
BstNSI RCATGY 1 cut(s) 223
BstSCI CCNGG 4 cut(s) 153, 240, 339, 496
BstSFI CTRYAG 1 cut(s) 454
BstUI CGCG 1 cut(s) 474
BstV1I GCAGC 1 cut(s) 130
BstX2I RGATCY 2 cut(s) 256, 289
BstYI RGATCY 2 cut(s) 256, 289
BsuRI GGCC 3 cut(s) 132, 176, 496
BtsCI GGATG 1 cut(s) 322
BtsIMutI CAGTG 2 cut(s) 357, 562
BtuMI TCGCGA 1 cut(s) 474
Cac8I GCNNGC 3 cut(s) 51, 225, 546
Cfr10I RCCGGY 2 cut(s) 49, 492
Csp6I GTAC 1 cut(s) 114
CviAII CATG 3 cut(s) 33, 220, 235
CviQI GTAC 1 cut(s) 114
DpnI GATC 4 cut(s) 188, 258, 291, 438
DpnII GATC 4 cut(s) 186, 256, 289, 436
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 2 cut(s) 140, 477
EarI CTCTTC 2 cut(s) 140, 477
EciI GGCGGA 2 cut(s) 20, 132
Eco130I CCWWGG 1 cut(s) 445
Eco24I GRGCYC 1 cut(s) 30
EcoNI CCTNNNNNAGG 1 cut(s) 419
EcoRII CCWGG 2 cut(s) 339, 496
EcoT14I CCWWGG 1 cut(s) 445
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 1 cut(s) 445
FaeI CATG 3 cut(s) 36, 223, 238
FaiI YATR 4 cut(s) 34, 221, 236, 287
FaqI GGGAC 3 cut(s) 346, 361, 397
FatI CATG 3 cut(s) 32, 219, 234
Fnu4HI GCNGC 2 cut(s) 18, 119
FokI GGATG 1 cut(s) 329
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 2 cut(s) 18, 119
GluI GCNGC 2 cut(s) 18, 119
HaeIII GGCC 3 cut(s) 132, 176, 496
HapII CCGG 4 cut(s) 50, 155, 242, 493
Hin1II CATG 3 cut(s) 36, 223, 238
HinfI GANTC 1 cut(s) 359
HpaII CCGG 4 cut(s) 50, 155, 242, 493
Hpy188I TCNGA 1 cut(s) 436
Hpy188III TCNNGA 1 cut(s) 473
HpyAV CCTTC 4 cut(s) 391, 400, 460, 552
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4V TGCA 5 cut(s) 227, 250, 320, 419, 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 46, 550, 559
Hsp92II CATG 3 cut(s) 36, 223, 238
KroI GCCGGC 1 cut(s) 49
KroNI GCCGGC 1 cut(s) 51
Kzo9I GATC 4 cut(s) 186, 256, 289, 436
LmnI GCTCC 2 cut(s) 37, 77
Lsp1109I GCAGC 1 cut(s) 130
LweI GCATC 1 cut(s) 307
MaeIII GTNAC 1 cut(s) 440
MalI GATC 4 cut(s) 188, 258, 291, 438
MboI GATC 4 cut(s) 186, 256, 289, 436
MboII GAAGA 4 cut(s) 127, 367, 494, 529
MfeI CAATTG 1 cut(s) 263
MflI RGATCY 2 cut(s) 256, 289
MhlI GDGCHC 1 cut(s) 30
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 2 cut(s) 170, 263
MluNI TGGCCA 1 cut(s) 176
MlyI GAGTC 1 cut(s) 368
MnlI CCTC 4 cut(s) 90, 143, 156, 415
Mox20I TGGCCA 1 cut(s) 176
MroNI GCCGGC 1 cut(s) 49
MscI TGGCCA 1 cut(s) 176
MseI TTAA 1 cut(s) 275
Msp20I TGGCCA 1 cut(s) 176
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 4 cut(s) 50, 155, 242, 493
MspR9I CCNGG 4 cut(s) 155, 242, 341, 498
MunI CAATTG 1 cut(s) 263
MvaI CCWGG 2 cut(s) 341, 498
MvnI CGCG 1 cut(s) 474
MwoI GCNNNNNNNGC 3 cut(s) 46, 550, 559
NaeI GCCGGC 1 cut(s) 51
NciI CCSGG 2 cut(s) 155, 242
NdeII GATC 4 cut(s) 186, 256, 289, 436
NgoMIV GCCGGC 1 cut(s) 49
NlaIII CATG 3 cut(s) 36, 223, 238
NlaIV GGNNCC 3 cut(s) 39, 73, 291
NruI TCGCGA 1 cut(s) 474
NspI RCATGY 1 cut(s) 223
PdiI GCCGGC 1 cut(s) 51
PkrI GCNGC 2 cut(s) 19, 120
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
Psp6I CCWGG 2 cut(s) 339, 496
PspGI CCWGG 2 cut(s) 339, 496
PspN4I GGNNCC 3 cut(s) 39, 73, 291
PsuI RGATCY 2 cut(s) 256, 289
PvuII CAGCTG 1 cut(s) 200
RruI TCGCGA 1 cut(s) 474
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
SaqAI TTAA 1 cut(s) 275
SatI GCNGC 2 cut(s) 18, 119
Sau3AI GATC 4 cut(s) 186, 256, 289, 436
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 4 cut(s) 155, 242, 341, 498
SduI GDGCHC 1 cut(s) 30
SetI ASST 8 cut(s) 82, 123, 202, 212, 345, 411, 447, 471
SfaNI GCATC 1 cut(s) 307
SfcI CTRYAG 1 cut(s) 454
SmlI CTYRAG 2 cut(s) 269, 379
SmoI CTYRAG 2 cut(s) 269, 379
Sse9I AATT 2 cut(s) 170, 263
SsiI CCGC 5 cut(s) 5, 17, 98, 143, 463
StyD4I CCNGG 4 cut(s) 153, 240, 339, 496
StyI CCWWGG 1 cut(s) 445
TaaI ACNGT 1 cut(s) 352
TaqI TCGA 1 cut(s) 126
TasI AATT 2 cut(s) 170, 263
TauI GCSGC 1 cut(s) 20
Tru1I TTAA 1 cut(s) 275
Tru9I TTAA 1 cut(s) 275
TscAI CASTG 1 cut(s) 357
TseI GCWGC 1 cut(s) 118
TspDTI ATGAA 2 cut(s) 48, 522
TspRI CASTG 1 cut(s) 357
XagI CCTNNNNNAGG 1 cut(s) 419
XceI RCATGY 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.