MD13G1196400.v1.1

Probable lipid transfer

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005475
Physical Location & Seq
Forward (+)
17071495 .. 17071854
360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1196400.v1.1.491

Sequence Viewer

Length: 360 bp
ATGGCCATCCTCCTAGCCTTGACGCTCGCCATTCTCGGGGCTGAAGCAGCAGAACCACCGCCGCCGCTACCTAGTTGCGCTGACCAGCTCGTGAGATTCTCTCCGTGCCTGCCGTACGTGTCCTCTCCGCCGAACAACCTCTCCGACTCGCCCCCACCCAAGTGCTGTGACGCGTTCTCGCTGTCGATGGAGTCCGGCGGCGCTCTTTGCCTCTGCTACCTGGTCCAGGATCCTCCCATGCTCGGGTTTCCGTTGAACCGGAGTCGCGTTCTGTCTCTGTCTTCCACTTGCCCTCTCAGAGATATTTCCACAAACACAAGCAGTGCACAATCTTTGGAATCGCTCTGCTCAGGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

120

Amino Acids

12.39

Weight (kDa)

4.36

Isoelectric Point (pI)

71.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 10 - 100 1.9e-13 Probable lipid transfer
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 173, 267
AciI CCGC 5 cut(s) 59, 62, 65, 128, 198
AclWI GGATC 2 cut(s) 224, 237
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 63
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 4 cut(s) 36, 226, 242, 243
AflIII ACRYGT 2 cut(s) 117, 171
AgsI TTSAA 1 cut(s) 256
AjnI CCWGG 2 cut(s) 219, 225
AleI CACNNNNGTG 1 cut(s) 160
AloI GAACNNNNNNTCC 2 cut(s) 125, 157
AluBI AGCT 1 cut(s) 88
AluI AGCT 1 cut(s) 88
Alw21I GWGCWC 1 cut(s) 328
Alw26I GTCTC 1 cut(s) 279
Alw44I GTGCAC 1 cut(s) 324
AlwI GGATC 2 cut(s) 224, 237
Ama87I CYCGRG 2 cut(s) 35, 242
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 324
ApeKI GCWGC 1 cut(s) 47
AspLEI GCGC 2 cut(s) 80, 203
AspS9I GGNCC 1 cut(s) 223
AvaI CYCGRG 2 cut(s) 35, 242
AvaII GGWCC 1 cut(s) 223
BaeGI GKGCMC 1 cut(s) 328
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 229
BauI CACGAG 1 cut(s) 89
BbsI GAAGAC 1 cut(s) 273
Bbv12I GWGCWC 1 cut(s) 328
BbvI GCAGC 1 cut(s) 59
BccI CCATC 2 cut(s) 14, 181
BceAI ACGGC 1 cut(s) 97
BciT130I CCWGG 2 cut(s) 221, 227
BcoDI GTCTC 1 cut(s) 279
BfaI CTAG 2 cut(s) 14, 72
BfoI RGCGCY 1 cut(s) 204
BisI GCNGC 4 cut(s) 48, 62, 65, 199
BlsI GCNGC 4 cut(s) 49, 63, 66, 200
Bme1390I CCNGG 2 cut(s) 221, 227
Bme18I GGWCC 1 cut(s) 223
BmeT110I CYCGRG 2 cut(s) 35, 242
BmgT120I GGNCC 1 cut(s) 223
BmiI GGNNCC 1 cut(s) 231
BmrFI CCNGG 2 cut(s) 221, 227
BpiI GAAGAC 1 cut(s) 273
BplI GAGNNNNNCTC 2 cut(s) 85, 117
Bpu10I CCTNAGC 1 cut(s) 349
BsaAI YACGTR 1 cut(s) 118
BsaWI WCCGGW 1 cut(s) 258
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 4 cut(s) 36, 226, 242, 243
BseBI CCWGG 2 cut(s) 221, 227
BseGI GGATG 1 cut(s) 6
BseLI CCNNNNNNNGG 4 cut(s) 36, 226, 242, 243
BseMII CTCAG 1 cut(s) 310
BseSI GKGCMC 1 cut(s) 328
BseXI GCAGC 1 cut(s) 59
Bsh1236I CGCG 2 cut(s) 173, 267
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 328
BsiHKCI CYCGRG 2 cut(s) 35, 242
BsiSI CCGG 2 cut(s) 195, 259
BsiWI CGTACG 1 cut(s) 114
BslI CCNNNNNNNGG 4 cut(s) 36, 226, 242, 243
BsmAI GTCTC 1 cut(s) 279
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 2 cut(s) 35, 242
Bsp1286I GDGCHC 1 cut(s) 328
Bsp143I GATC 1 cut(s) 229
BspACI CCGC 5 cut(s) 59, 62, 65, 128, 198
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 309
BspFNI CGCG 2 cut(s) 173, 267
BspLI GGNNCC 1 cut(s) 231
BspPI GGATC 2 cut(s) 224, 237
BssMI GATC 1 cut(s) 229
BssSI CACGAG 1 cut(s) 89
Bst2BI CACGAG 1 cut(s) 89
Bst2UI CCWGG 2 cut(s) 221, 227
BstBAI YACGTR 1 cut(s) 118
BstC8I GCNNGC 2 cut(s) 27, 110
BstDEI CTNAG 2 cut(s) 296, 349
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 1 cut(s) 6
BstFNI CGCG 2 cut(s) 173, 267
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 2 cut(s) 80, 203
BstKTI GATC 1 cut(s) 232
BstMAI GTCTC 1 cut(s) 279
BstMBI GATC 1 cut(s) 229
BstMWI GCNNNNNNNGC 2 cut(s) 47, 207
BstNI CCWGG 2 cut(s) 221, 227
BstSCI CCNGG 2 cut(s) 219, 225
BstSLI GKGCMC 1 cut(s) 328
BstUI CGCG 2 cut(s) 173, 267
BstV1I GCAGC 1 cut(s) 59
BstV2I GAAGAC 1 cut(s) 273
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 6
BtsI GCAGTG 1 cut(s) 328
BtsIMutI CAGTG 1 cut(s) 328
Cac8I GCNNGC 2 cut(s) 27, 110
CfoI GCGC 2 cut(s) 80, 203
Cfr13I GGNCC 1 cut(s) 223
CseI GACGC 2 cut(s) 31, 179
CsiI ACCWGGT 1 cut(s) 219
Csp6I GTAC 1 cut(s) 115
CviAII CATG 1 cut(s) 238
CviJI RGCY 4 cut(s) 5, 17, 41, 88
CviKI_1 RGCY 4 cut(s) 5, 17, 41, 88
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 2 cut(s) 296, 349
DpnI GATC 1 cut(s) 231
DpnII GATC 1 cut(s) 229
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 117
Eco47I GGWCC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 63
Eco88I CYCGRG 2 cut(s) 35, 242
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoRII CCWGG 2 cut(s) 219, 225
FaeI CATG 1 cut(s) 241
FaiI YATR 1 cut(s) 239
FatI CATG 1 cut(s) 237
Fnu4HI GCNGC 4 cut(s) 48, 62, 65, 199
Fsp4HI GCNGC 4 cut(s) 48, 62, 65, 199
FspBI CTAG 2 cut(s) 14, 72
GlaI GCGC 2 cut(s) 79, 202
GluI GCNGC 4 cut(s) 48, 62, 65, 199
HaeII RGCGCY 1 cut(s) 204
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 195, 259
HgaI GACGC 2 cut(s) 31, 179
HhaI GCGC 2 cut(s) 80, 203
Hin1II CATG 1 cut(s) 241
Hin6I GCGC 2 cut(s) 78, 201
HinP1I GCGC 2 cut(s) 78, 201
HinfI GANTC 5 cut(s) 96, 146, 191, 262, 338
HpaII CCGG 2 cut(s) 195, 259
Hpy166II GTNNAC 1 cut(s) 326
Hpy188I TCNGA 2 cut(s) 145, 299
Hpy188III TCNNGA 2 cut(s) 91, 357
Hpy8I GTNNAC 1 cut(s) 326
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 1 cut(s) 326
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 207
HpyF3I CTNAG 2 cut(s) 296, 349
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 1 cut(s) 241
HspAI GCGC 2 cut(s) 78, 201
Kzo9I GATC 1 cut(s) 229
LpnPI CCDG 9 cut(s) 98, 122, 206, 208, 212, 233, 239, 272, 336
Lsp1109I GCAGC 1 cut(s) 59
MabI ACCWGGT 1 cut(s) 219
MaeI CTAG 2 cut(s) 14, 72
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 1 cut(s) 167
MalI GATC 1 cut(s) 231
MboI GATC 1 cut(s) 229
MboII GAAGA 1 cut(s) 273
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 328
MlsI TGGCCA 1 cut(s) 5
MluI ACGCGT 1 cut(s) 171
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 140, 200, 271
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 6 cut(s) 20, 133, 149, 221, 243, 303
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 160
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 195, 259
MspR9I CCNGG 2 cut(s) 221, 227
MvaI CCWGG 2 cut(s) 221, 227
MvnI CGCG 2 cut(s) 173, 267
MwoI GCNNNNNNNGC 2 cut(s) 47, 207
NdeII GATC 1 cut(s) 229
NlaIII CATG 1 cut(s) 241
NlaIV GGNNCC 1 cut(s) 231
NmuCI GTSAC 1 cut(s) 167
OliI CACNNNNGTG 1 cut(s) 160
PfeI GAWTC 2 cut(s) 96, 338
Pfl23II CGTACG 1 cut(s) 114
PfoI TCCNGGA 1 cut(s) 225
PkrI GCNGC 4 cut(s) 49, 63, 66, 200
PleI GAGTC 3 cut(s) 140, 199, 270
PpsI GAGTC 3 cut(s) 140, 199, 270
Ppu21I YACGTR 1 cut(s) 118
Psp6I CCWGG 2 cut(s) 219, 225
PspGI CCWGG 2 cut(s) 219, 225
PspLI CGTACG 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 231
PspPI GGNCC 1 cut(s) 223
PsuI RGATCY 1 cut(s) 229
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
RseI CAYNNNNRTG 1 cut(s) 160
SatI GCNGC 4 cut(s) 48, 62, 65, 199
Sau3AI GATC 1 cut(s) 229
Sau96I GGNCC 1 cut(s) 223
SchI GAGTC 3 cut(s) 140, 200, 271
ScrFI CCNGG 2 cut(s) 221, 227
SduI GDGCHC 1 cut(s) 328
SetI ASST 6 cut(s) 73, 90, 120, 141, 222, 355
SexAI ACCWGGT 1 cut(s) 219
SinI GGWCC 1 cut(s) 223
SmiMI CAYNNNNRTG 1 cut(s) 160
SsiI CCGC 5 cut(s) 59, 62, 65, 128, 198
SspMI CTAG 2 cut(s) 14, 72
StyD4I CCNGG 2 cut(s) 219, 225
TaiI ACGT 1 cut(s) 120
TaqI TCGA 1 cut(s) 185
TauI GCSGC 3 cut(s) 64, 67, 201
TfiI GAWTC 2 cut(s) 96, 338
TscAI CASTG 1 cut(s) 328
TseFI GTSAC 1 cut(s) 167
TseI GCWGC 1 cut(s) 47
Tsp45I GTSAC 1 cut(s) 167
TspGWI ACGGA 2 cut(s) 93, 240
TspRI CASTG 1 cut(s) 328
VneI GTGCAC 1 cut(s) 324
VpaK11BI GGWCC 1 cut(s) 223
XagI CCTNNNNNAGG 1 cut(s) 224
XspI CTAG 2 cut(s) 14, 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.