Rroxscaffold_5G00359400

Probable lipid transfer

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005475
Physical Location & Seq
Reverse (-)
39618765 .. 39619376
612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00359400.1

Sequence Viewer

Length: 504 bp
ATGAGCACCACCGCCACAGCCACCGCTCTGCGCTCCTCGTCTCTCATGGCCGTCCTCCTCCTCGCCCCCTTGTTCGTCACTCTCGGCGCCGCAAAGTCAGCAGAAGAATCGCCGCCGTCCGCTTGCGCCGACGAGCTCGTCCGGTTCTCGCCGTGCCTGTCGTACGTGGCGTCGGCGCCGAACAACCTCTCCGACTCGCCGGCCTCCAAGTGCTGCGACGCGTTCTCGTCTGCGGCGGAGTCCGGCAGCGCCGTTTGCCTCTGCTACCTAGTCCAGGATCCCCCGATGCTCGGCTTTCCGGTGACCGTCGCTCGCGTCCTCTCGCTCTCTGCCGTTTGCCGTCGCCGGAACTCCACCGCTGCTTCTACTTCTCTTCAGTCCCTTTGCTCAGAGTCGCCGGAACTCCCCTCCTCTCCGCAACACCACGATTTCGGTGATTTCAGGTCCTCCTCCTTCTGGTACTGTCTCACTCACTTCATCATTACCTTGATTATTTTACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

167

Amino Acids

17.57

Weight (kDa)

5.48

Isoelectric Point (pI)

79.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 32 - 119 7.7e-14 Probable lipid transfer
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 137
AccB1I GGYRCC 2 cut(s) 86, 175
AccBSI CCGCTC 1 cut(s) 26
AccII CGCG 2 cut(s) 221, 315
AciI CCGC 9 cut(s) 12, 24, 90, 113, 120, 233, 236, 357, 416
AclWI GGATC 2 cut(s) 272, 285
AcoI YGGCCR 1 cut(s) 48
AcuI CTGAAG 1 cut(s) 359
AcyI GRCGYC 3 cut(s) 87, 170, 176
AfaI GTAC 2 cut(s) 164, 461
AfiI CCNNNNNNNGG 3 cut(s) 274, 290, 456
AflIII ACRYGT 1 cut(s) 219
AjnI CCWGG 1 cut(s) 273
AloI GAACNNNNNNTCC 2 cut(s) 173, 205
AluBI AGCT 1 cut(s) 136
AluI AGCT 1 cut(s) 136
Alw21I GWGCWC 2 cut(s) 8, 138
Alw26I GTCTC 2 cut(s) 45, 470
AlwI GGATC 2 cut(s) 272, 285
AoxI GGCC 2 cut(s) 48, 201
ApeKI GCWGC 3 cut(s) 213, 246, 359
AspLEI GCGC 5 cut(s) 33, 89, 128, 178, 251
AspS9I GGNCC 1 cut(s) 444
AsuHPI GGTGA 2 cut(s) 313, 446
AvaII GGWCC 1 cut(s) 444
BamHI GGATCC 1 cut(s) 277
BanI GGYRCC 2 cut(s) 86, 175
BanII GRGCYC 1 cut(s) 138
Bbv12I GWGCWC 2 cut(s) 8, 138
BbvI GCAGC 3 cut(s) 200, 258, 346
BceAI ACGGC 6 cut(s) 35, 100, 136, 236, 317, 324
BcgI CGANNNNNNTGC 2 cut(s) 90, 124
BciT130I CCWGG 1 cut(s) 275
BcoDI GTCTC 2 cut(s) 45, 470
BfaI CTAG 1 cut(s) 269
BfoI RGCGCY 3 cut(s) 90, 179, 252
BisI GCNGC 6 cut(s) 90, 113, 214, 234, 247, 360
BlsI GCNGC 6 cut(s) 91, 114, 215, 235, 248, 361
Bme1390I CCNGG 1 cut(s) 275
Bme18I GGWCC 1 cut(s) 444
BmgT120I GGNCC 1 cut(s) 444
BmiI GGNNCC 3 cut(s) 88, 177, 279
BmrFI CCNGG 1 cut(s) 275
BmsI GCATC 1 cut(s) 276
BsaAI YACGTR 1 cut(s) 166
BsaHI GRCGYC 3 cut(s) 87, 170, 176
BsaWI WCCGGW 2 cut(s) 141, 298
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 3 cut(s) 274, 290, 456
Bse118I RCCGGY 1 cut(s) 199
BseBI CCWGG 1 cut(s) 275
BseLI CCNNNNNNNGG 3 cut(s) 274, 290, 456
BseMII CTCAG 1 cut(s) 402
BseRI GAGGAG 5 cut(s) 25, 47, 50, 400, 439
BseXI GCAGC 3 cut(s) 200, 258, 346
Bsh1236I CGCG 2 cut(s) 221, 315
BshFI GGCC 2 cut(s) 50, 203
BshNI GGYRCC 2 cut(s) 86, 175
BsiHKAI GWGCWC 2 cut(s) 8, 138
BsiSI CCGG 6 cut(s) 142, 200, 243, 299, 346, 398
BsiWI CGTACG 1 cut(s) 162
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 3 cut(s) 274, 290, 456
BsmAI GTCTC 2 cut(s) 45, 470
BsmBI CGTCTC 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 364
BsnI GGCC 2 cut(s) 50, 203
Bsp1286I GDGCHC 2 cut(s) 8, 138
Bsp143I GATC 1 cut(s) 277
BspACI CCGC 9 cut(s) 12, 24, 90, 113, 120, 233, 236, 357, 416
BspANI GGCC 2 cut(s) 50, 203
BspCNI CTCAG 1 cut(s) 401
BspFNI CGCG 2 cut(s) 221, 315
BspLI GGNNCC 3 cut(s) 88, 177, 279
BspPI GGATC 2 cut(s) 272, 285
BspT107I GGYRCC 2 cut(s) 86, 175
BsrBI CCGCTC 1 cut(s) 26
BsrFI RCCGGY 1 cut(s) 199
BssAI RCCGGY 1 cut(s) 199
BssMI GATC 1 cut(s) 277
BssNI GRCGYC 3 cut(s) 87, 170, 176
Bst2UI CCWGG 1 cut(s) 275
Bst4CI ACNGT 2 cut(s) 307, 464
Bst6I CTCTTC 1 cut(s) 378
BstACI GRCGYC 3 cut(s) 87, 170, 176
BstBAI YACGTR 1 cut(s) 166
BstC8I GCNNGC 3 cut(s) 124, 201, 313
BstDEI CTNAG 1 cut(s) 388
BstEII GGTNACC 1 cut(s) 301
BstENI CCTNNNNNAGG 1 cut(s) 272
BstFNI CGCG 2 cut(s) 221, 315
BstH2I RGCGCY 3 cut(s) 90, 179, 252
BstHHI GCGC 5 cut(s) 33, 89, 128, 178, 251
BstKTI GATC 1 cut(s) 280
BstMAI GTCTC 2 cut(s) 45, 470
BstMBI GATC 1 cut(s) 277
BstMWI GCNNNNNNNGC 2 cut(s) 98, 255
BstNI CCWGG 1 cut(s) 275
BstPI GGTNACC 1 cut(s) 301
BstSCI CCNGG 1 cut(s) 273
BstUI CGCG 2 cut(s) 221, 315
BstV1I GCAGC 3 cut(s) 200, 258, 346
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BsuRI GGCC 2 cut(s) 50, 203
Cac8I GCNNGC 3 cut(s) 124, 201, 313
CfoI GCGC 5 cut(s) 33, 89, 128, 178, 251
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 1 cut(s) 444
CseI GACGC 3 cut(s) 159, 227, 304
Csp6I GTAC 2 cut(s) 163, 460
CviAII CATG 1 cut(s) 46
CviJI RGCY 5 cut(s) 20, 50, 136, 203, 294
CviKI_1 RGCY 5 cut(s) 20, 50, 136, 203, 294
CviQI GTAC 2 cut(s) 163, 460
DdeI CTNAG 1 cut(s) 388
DinI GGCGCC 2 cut(s) 88, 177
DpnI GATC 1 cut(s) 279
DpnII GATC 1 cut(s) 277
DrdI GACNNNNNNGTC 1 cut(s) 137
DseDI GACNNNNNNGTC 1 cut(s) 137
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 378
EarI CTCTTC 1 cut(s) 378
EciI GGCGGA 1 cut(s) 251
Ecl136II GAGCTC 1 cut(s) 136
Eco24I GRGCYC 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 444
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 359
Eco91I GGTNACC 1 cut(s) 301
EcoICRI GAGCTC 1 cut(s) 136
EcoNI CCTNNNNNAGG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 444
EcoO65I GGTNACC 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 273
EcoT38I GRGCYC 1 cut(s) 138
EgeI GGCGCC 2 cut(s) 88, 177
EheI GGCGCC 2 cut(s) 88, 177
Esp3I CGTCTC 1 cut(s) 45
FaeI CATG 1 cut(s) 49
FaiI YATR 1 cut(s) 47
FaqI GGGAC 1 cut(s) 364
FatI CATG 1 cut(s) 45
Fnu4HI GCNGC 6 cut(s) 90, 113, 214, 234, 247, 360
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 6 cut(s) 90, 113, 214, 234, 247, 360
FspBI CTAG 1 cut(s) 269
GlaI GCGC 5 cut(s) 32, 88, 127, 177, 250
GluI GCNGC 6 cut(s) 90, 113, 214, 234, 247, 360
HaeII RGCGCY 3 cut(s) 90, 179, 252
HaeIII GGCC 2 cut(s) 50, 203
HapII CCGG 6 cut(s) 142, 200, 243, 299, 346, 398
HgaI GACGC 3 cut(s) 159, 227, 304
HhaI GCGC 5 cut(s) 33, 89, 128, 178, 251
Hin1I GRCGYC 3 cut(s) 87, 170, 176
Hin1II CATG 1 cut(s) 49
Hin6I GCGC 5 cut(s) 31, 87, 126, 176, 249
HinP1I GCGC 5 cut(s) 31, 87, 126, 176, 249
HinfI GANTC 4 cut(s) 107, 194, 239, 392
HpaII CCGG 6 cut(s) 142, 200, 243, 299, 346, 398
HphI GGTGA 2 cut(s) 313, 446
Hpy188I TCNGA 2 cut(s) 193, 391
Hpy99I CGWCG 5 cut(s) 134, 175, 221, 311, 345
HpyAV CCTTC 1 cut(s) 463
HpyCH4III ACNGT 2 cut(s) 307, 464
HpyCH4IV ACGT 1 cut(s) 165
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 255
HpyF3I CTNAG 1 cut(s) 388
HpySE526I ACGT 1 cut(s) 165
Hsp92I GRCGYC 3 cut(s) 87, 170, 176
Hsp92II CATG 1 cut(s) 49
HspAI GCGC 5 cut(s) 31, 87, 126, 176, 249
KasI GGCGCC 2 cut(s) 86, 175
KroI GCCGGC 1 cut(s) 199
KroNI GCCGGC 1 cut(s) 201
Kzo9I GATC 1 cut(s) 277
LmnI GCTCC 1 cut(s) 38
Lsp1109I GCAGC 3 cut(s) 200, 258, 346
LweI GCATC 1 cut(s) 276
MaeI CTAG 1 cut(s) 269
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 2 cut(s) 76, 301
MalI GATC 1 cut(s) 279
MbiI CCGCTC 1 cut(s) 26
MboI GATC 1 cut(s) 277
MboII GAAGA 2 cut(s) 116, 365
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 2 cut(s) 8, 138
MluI ACGCGT 1 cut(s) 219
Mly113I GGCGCC 2 cut(s) 87, 176
MlyI GAGTC 3 cut(s) 188, 248, 401
MmeI TCCRAC 1 cut(s) 216
MroNI GCCGGC 1 cut(s) 199
MseI TTAA 1 cut(s) 502
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 6 cut(s) 142, 200, 243, 299, 346, 398
MspR9I CCNGG 1 cut(s) 275
MvaI CCWGG 1 cut(s) 275
MvnI CGCG 2 cut(s) 221, 315
MwoI GCNNNNNNNGC 2 cut(s) 98, 255
NaeI GCCGGC 1 cut(s) 201
NarI GGCGCC 2 cut(s) 87, 176
NdeII GATC 1 cut(s) 277
NgoMIV GCCGGC 1 cut(s) 199
NlaIII CATG 1 cut(s) 49
NlaIV GGNNCC 3 cut(s) 88, 177, 279
NmeAIII GCCGAG 2 cut(s) 63, 270
NmuCI GTSAC 2 cut(s) 76, 301
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PdiI GCCGGC 1 cut(s) 201
PfeI GAWTC 1 cut(s) 107
Pfl23II CGTACG 1 cut(s) 162
PfoI TCCNGGA 1 cut(s) 273
PkrI GCNGC 6 cut(s) 91, 114, 215, 235, 248, 361
PleI GAGTC 3 cut(s) 188, 247, 400
PluTI GGCGCC 2 cut(s) 90, 179
PpsI GAGTC 3 cut(s) 188, 247, 400
Ppu21I YACGTR 1 cut(s) 166
PpuMI RGGWCCY 1 cut(s) 444
Psp124BI GAGCTC 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 273
PspEI GGTNACC 1 cut(s) 301
PspGI CCWGG 1 cut(s) 273
PspLI CGTACG 1 cut(s) 162
PspN4I GGNNCC 3 cut(s) 88, 177, 279
PspPI GGNCC 1 cut(s) 444
PspPPI RGGWCCY 1 cut(s) 444
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 2 cut(s) 164, 461
RsaNI GTAC 2 cut(s) 163, 460
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 1 cut(s) 502
SatI GCNGC 6 cut(s) 90, 113, 214, 234, 247, 360
Sau3AI GATC 1 cut(s) 277
Sau96I GGNCC 1 cut(s) 444
SchI GAGTC 3 cut(s) 188, 248, 401
ScrFI CCNGG 1 cut(s) 275
SduI GDGCHC 2 cut(s) 8, 138
SetI ASST 7 cut(s) 138, 168, 189, 270, 446, 488, 502
SfaNI GCATC 1 cut(s) 276
SfoI GGCGCC 2 cut(s) 88, 177
SinI GGWCC 1 cut(s) 444
SsiI CCGC 9 cut(s) 12, 24, 90, 113, 120, 233, 236, 357, 416
SspDI GGCGCC 2 cut(s) 86, 175
SspMI CTAG 1 cut(s) 269
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 1 cut(s) 273
TaaI ACNGT 2 cut(s) 307, 464
TaiI ACGT 1 cut(s) 168
TauI GCSGC 3 cut(s) 92, 115, 236
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 1 cut(s) 502
Tru9I TTAA 1 cut(s) 502
TseFI GTSAC 2 cut(s) 76, 301
TseI GCWGC 3 cut(s) 213, 246, 359
Tsp45I GTSAC 2 cut(s) 76, 301
TspDTI ATGAA 1 cut(s) 466
VpaK11BI GGWCC 1 cut(s) 444
XagI CCTNNNNNAGG 1 cut(s) 272
XspI CTAG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.