MD16G1196900.v1.1

Probable lipid transfer

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005475
Physical Location & Seq
Forward (+)
17499325 .. 17499717
393 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1196900.v1.1.491

Sequence Viewer

Length: 393 bp
ATGATGACCACCACTACTGCTCCTCCTCCTCTCATGGCCATCCTCCTAGCCTTGACGCTCGTCATTCTCCGCTTGGGATCAGCAGAACCGCCGCCGTCTCCACCACCTAGTTGCGCCGACGAGCTCGTGAGATTCTCGCCGTGCCTGCCTTACGTGTCGTCTCCGCCAAACAACATCTCCGACTCACCCCCACCCAAGTGCTGCGACGCGTTCTCGCTGTCGATGGAGTCCGGCGGCGCGCTCTGCCTCTGCTACCTGGTCCAGGATCCTCCGATGCTGGGGTTTCCGGTGAACGGGAGTCGCGTTCTGTCTCTGTCTTCCACTTGCCCTCTCAGAGACATTTCCACAAACGCAAGTGCACAATCTTTGGAGTCTCTCTGCTCAGGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

131

Amino Acids

13.48

Weight (kDa)

4.36

Isoelectric Point (pI)

76.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 30 - 112 3.6e-13 Probable lipid transfer
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 209, 239, 303
AciI CCGC 5 cut(s) 70, 89, 92, 164, 234
AclWI GGATC 3 cut(s) 85, 260, 273
AcoI YGGCCR 1 cut(s) 36
AfiI CCNNNNNNNGG 3 cut(s) 262, 278, 293
AflIII ACRYGT 2 cut(s) 153, 207
AjnI CCWGG 2 cut(s) 255, 261
AleI CACNNNNGTG 1 cut(s) 196
AluBI AGCT 1 cut(s) 124
AluI AGCT 1 cut(s) 124
Alw21I GWGCWC 2 cut(s) 126, 361
Alw26I GTCTC 5 cut(s) 102, 165, 315, 330, 378
Alw44I GTGCAC 1 cut(s) 357
AlwI GGATC 3 cut(s) 85, 260, 273
AoxI GGCC 1 cut(s) 36
ApaLI GTGCAC 1 cut(s) 357
ApeKI GCWGC 1 cut(s) 201
AspLEI GCGC 3 cut(s) 116, 239, 241
AspS9I GGNCC 1 cut(s) 259
AsuHPI GGTGA 2 cut(s) 177, 301
AvaII GGWCC 1 cut(s) 259
BaeGI GKGCMC 1 cut(s) 361
BalI TGGCCA 1 cut(s) 38
BamHI GGATCC 1 cut(s) 265
BanII GRGCYC 1 cut(s) 126
BauI CACGAG 1 cut(s) 125
BbsI GAAGAC 1 cut(s) 309
Bbv12I GWGCWC 2 cut(s) 126, 361
BbvI GCAGC 1 cut(s) 188
BccI CCATC 2 cut(s) 47, 217
BceAI ACGGC 2 cut(s) 79, 124
BciT130I CCWGG 2 cut(s) 257, 263
BcoDI GTCTC 5 cut(s) 102, 165, 315, 330, 378
BfaI CTAG 2 cut(s) 47, 108
BisI GCNGC 3 cut(s) 92, 202, 235
BlsI GCNGC 3 cut(s) 93, 203, 236
Bme1390I CCNGG 2 cut(s) 257, 263
Bme18I GGWCC 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 1 cut(s) 267
BmrFI CCNGG 2 cut(s) 257, 263
BmsI GCATC 1 cut(s) 264
BoxI GACNNNNGTC 1 cut(s) 59
BpiI GAAGAC 1 cut(s) 309
Bpu10I CCTNAGC 1 cut(s) 382
BsaAI YACGTR 1 cut(s) 154
BsaWI WCCGGW 1 cut(s) 286
BsaXI ACNNNNNCTCC 5 cut(s) 7, 34, 37, 161, 191
Bsc4I CCNNNNNNNGG 3 cut(s) 262, 278, 293
BseBI CCWGG 2 cut(s) 257, 263
BseGI GGATG 1 cut(s) 39
BseLI CCNNNNNNNGG 3 cut(s) 262, 278, 293
BseMII CTCAG 1 cut(s) 346
BsePI GCGCGC 1 cut(s) 237
BseRI GAGGAG 3 cut(s) 12, 15, 18
BseSI GKGCMC 1 cut(s) 361
BseXI GCAGC 1 cut(s) 188
BseYI CCCAGC 1 cut(s) 277
Bsh1236I CGCG 3 cut(s) 209, 239, 303
BshFI GGCC 1 cut(s) 38
BsiHKAI GWGCWC 2 cut(s) 126, 361
BsiSI CCGG 2 cut(s) 231, 287
BslI CCNNNNNNNGG 3 cut(s) 262, 278, 293
BsmAI GTCTC 5 cut(s) 102, 165, 315, 330, 378
BsmBI CGTCTC 2 cut(s) 102, 165
BsnI GGCC 1 cut(s) 38
Bsp1286I GDGCHC 2 cut(s) 126, 361
Bsp143I GATC 2 cut(s) 77, 265
BspACI CCGC 5 cut(s) 70, 89, 92, 164, 234
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 1 cut(s) 345
BspFNI CGCG 3 cut(s) 209, 239, 303
BspLI GGNNCC 1 cut(s) 267
BspPI GGATC 3 cut(s) 85, 260, 273
BssHII GCGCGC 1 cut(s) 237
BssMI GATC 2 cut(s) 77, 265
BssSI CACGAG 1 cut(s) 125
Bst2BI CACGAG 1 cut(s) 125
Bst2UI CCWGG 2 cut(s) 257, 263
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 2 cut(s) 146, 239
BstDEI CTNAG 2 cut(s) 332, 382
BstENI CCTNNNNNAGG 1 cut(s) 260
BstF5I GGATG 1 cut(s) 39
BstFNI CGCG 3 cut(s) 209, 239, 303
BstHHI GCGC 3 cut(s) 116, 239, 241
BstKTI GATC 2 cut(s) 80, 268
BstMAI GTCTC 5 cut(s) 102, 165, 315, 330, 378
BstMBI GATC 2 cut(s) 77, 265
BstMWI GCNNNNNNNGC 2 cut(s) 145, 243
BstNI CCWGG 2 cut(s) 257, 263
BstPAI GACNNNNGTC 1 cut(s) 59
BstSCI CCNGG 2 cut(s) 255, 261
BstSLI GKGCMC 1 cut(s) 361
BstUI CGCG 3 cut(s) 209, 239, 303
BstV1I GCAGC 1 cut(s) 188
BstV2I GAAGAC 1 cut(s) 309
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BsuRI GGCC 1 cut(s) 38
BtsCI GGATG 1 cut(s) 39
Cac8I GCNNGC 2 cut(s) 146, 239
CfoI GCGC 3 cut(s) 116, 239, 241
Cfr13I GGNCC 1 cut(s) 259
CseI GACGC 2 cut(s) 64, 215
CsiI ACCWGGT 1 cut(s) 255
CviAII CATG 1 cut(s) 34
CviJI RGCY 3 cut(s) 38, 50, 124
CviKI_1 RGCY 3 cut(s) 38, 50, 124
DdeI CTNAG 2 cut(s) 332, 382
DpnI GATC 2 cut(s) 79, 267
DpnII GATC 2 cut(s) 77, 265
EaeI YGGCCR 1 cut(s) 36
EciI GGCGGA 1 cut(s) 153
Ecl136II GAGCTC 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 259
Eco53kI GAGCTC 1 cut(s) 124
EcoICRI GAGCTC 1 cut(s) 124
EcoNI CCTNNNNNAGG 1 cut(s) 260
EcoRII CCWGG 2 cut(s) 255, 261
EcoT38I GRGCYC 1 cut(s) 126
Esp3I CGTCTC 2 cut(s) 102, 165
FaeI CATG 1 cut(s) 37
FaiI YATR 1 cut(s) 35
FatI CATG 1 cut(s) 33
Fnu4HI GCNGC 3 cut(s) 92, 202, 235
FokI GGATG 1 cut(s) 26
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 3 cut(s) 92, 202, 235
FspBI CTAG 2 cut(s) 47, 108
GlaI GCGC 3 cut(s) 115, 238, 240
GluI GCNGC 3 cut(s) 92, 202, 235
GsaI CCCAGC 1 cut(s) 281
HaeIII GGCC 1 cut(s) 38
HapII CCGG 2 cut(s) 231, 287
HgaI GACGC 2 cut(s) 64, 215
HhaI GCGC 3 cut(s) 116, 239, 241
Hin1II CATG 1 cut(s) 37
Hin6I GCGC 3 cut(s) 114, 237, 239
HinP1I GCGC 3 cut(s) 114, 237, 239
HinfI GANTC 5 cut(s) 132, 182, 227, 298, 371
HpaII CCGG 2 cut(s) 231, 287
HphI GGTGA 2 cut(s) 177, 301
Hpy166II GTNNAC 2 cut(s) 292, 359
Hpy188I TCNGA 3 cut(s) 181, 273, 335
Hpy188III TCNNGA 2 cut(s) 127, 390
Hpy8I GTNNAC 2 cut(s) 292, 359
Hpy99I CGWCG 2 cut(s) 122, 209
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 1 cut(s) 359
HpyF10VI GCNNNNNNNGC 2 cut(s) 145, 243
HpyF3I CTNAG 2 cut(s) 332, 382
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 1 cut(s) 37
HspAI GCGC 3 cut(s) 114, 237, 239
Kzo9I GATC 2 cut(s) 77, 265
LmnI GCTCC 1 cut(s) 25
LpnPI CCDG 9 cut(s) 158, 242, 244, 248, 263, 269, 275, 300, 369
Lsp1109I GCAGC 1 cut(s) 188
LweI GCATC 1 cut(s) 264
MabI ACCWGGT 1 cut(s) 255
MaeI CTAG 2 cut(s) 47, 108
MaeII ACGT 1 cut(s) 153
MalI GATC 2 cut(s) 79, 267
MboI GATC 2 cut(s) 77, 265
MboII GAAGA 1 cut(s) 309
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 2 cut(s) 126, 361
MlsI TGGCCA 1 cut(s) 38
MluI ACGCGT 1 cut(s) 207
MluNI TGGCCA 1 cut(s) 38
MlyI GAGTC 4 cut(s) 176, 236, 307, 380
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 7 cut(s) 33, 36, 39, 53, 257, 279, 339
Mox20I TGGCCA 1 cut(s) 38
MscI TGGCCA 1 cut(s) 38
MslI CAYNNNNRTG 1 cut(s) 196
Msp20I TGGCCA 1 cut(s) 38
MspI CCGG 2 cut(s) 231, 287
MspR9I CCNGG 2 cut(s) 257, 263
MvaI CCWGG 2 cut(s) 257, 263
MvnI CGCG 3 cut(s) 209, 239, 303
MwoI GCNNNNNNNGC 2 cut(s) 145, 243
NdeII GATC 2 cut(s) 77, 265
NlaIII CATG 1 cut(s) 37
NlaIV GGNNCC 1 cut(s) 267
OliI CACNNNNGTG 1 cut(s) 196
PauI GCGCGC 1 cut(s) 237
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PfeI GAWTC 1 cut(s) 132
PfoI TCCNGGA 1 cut(s) 261
PkrI GCNGC 3 cut(s) 93, 203, 236
PleI GAGTC 4 cut(s) 176, 235, 306, 379
PpsI GAGTC 4 cut(s) 176, 235, 306, 379
Ppu21I YACGTR 1 cut(s) 154
PshAI GACNNNNGTC 1 cut(s) 59
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 2 cut(s) 255, 261
PspFI CCCAGC 1 cut(s) 277
PspGI CCWGG 2 cut(s) 255, 261
PspN4I GGNNCC 1 cut(s) 267
PspPI GGNCC 1 cut(s) 259
PsuI RGATCY 1 cut(s) 265
PteI GCGCGC 1 cut(s) 237
RseI CAYNNNNRTG 1 cut(s) 196
SacI GAGCTC 1 cut(s) 126
SatI GCNGC 3 cut(s) 92, 202, 235
Sau3AI GATC 2 cut(s) 77, 265
Sau96I GGNCC 1 cut(s) 259
SchI GAGTC 4 cut(s) 176, 236, 307, 380
ScrFI CCNGG 2 cut(s) 257, 263
SduI GDGCHC 2 cut(s) 126, 361
SetI ASST 5 cut(s) 109, 126, 156, 258, 388
SexAI ACCWGGT 1 cut(s) 255
SfaNI GCATC 1 cut(s) 264
SinI GGWCC 1 cut(s) 259
SmiMI CAYNNNNRTG 1 cut(s) 196
SsiI CCGC 5 cut(s) 70, 89, 92, 164, 234
SspMI CTAG 2 cut(s) 47, 108
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 2 cut(s) 255, 261
TaiI ACGT 1 cut(s) 156
TaqI TCGA 1 cut(s) 221
TauI GCSGC 2 cut(s) 94, 237
TfiI GAWTC 1 cut(s) 132
TseI GCWGC 1 cut(s) 201
VneI GTGCAC 1 cut(s) 357
VpaK11BI GGWCC 1 cut(s) 259
XagI CCTNNNNNAGG 1 cut(s) 260
XspI CTAG 2 cut(s) 47, 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.