MD13G1264600.v1.1
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002882
Physical Location & Seq
Reverse (-)
34155843 .. 34161586
5744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1264600.v1.1.491

Sequence Viewer

Length: 549 bp
ATGGGCATTTTGTTCACTAAAATGTTCTCTTCCCTGTTTGGGAACAAGGAAGCTCGGATCCTCGTCCTCGGATTGGACAATGCTGGCAAAACCACCATTCTCTATCGGCTCCAGATGGGGGAGGTTGTCTCCACGATCCCAACGATTGGATTTAATGTTGAGACAGTGCAGTACAACAACATCAAGTTCCAAGTCTGGGATCTCGGTGGACAGACAAGTATCAGGCCATATTGGAGATGCTATTTTCCAAATACTCAGGCCATAATCTATGTTGTTGATTCTAGTGACACCGATAGGCTGGTAATAGCTAAAGACGAGTTTCATGCAATACTGGAGGAGGAAGAGTTGAAAGGTGCAGTTGTCGTCATTTTTGCCAATAAGCAGGATCTTCCTGGTGCACTTGATGATGCTGCAATAACTGAGGCTTTAGAGTTGCACAAGATAAAAAATCGCCAGTGGGCTATTTTTAAAACTTCTGCCATAAAAGGGGAAGGCCTATTTGAGGGTTTAGACTGGTTGAGTAACACACTCAAGTCCGGAGGTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arf

Protein Analysis

183

Amino Acids

20.29

Weight (kDa)

5.24

Isoelectric Point (pI)

28.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 5 - 176 4.7e-79 ADP-ribosylation factor family
SRPRB PF09439 16 - 153 2.7e-12 Signal recognition particle receptor beta subunit
Roc PF08477 19 - 129 9.6e-14 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 19 - 171 2.4e-11 Ras family
Gtr1_RagA PF04670 19 - 130 7.4e-09 Gtr1/RagA G protein conserved region
G-alpha PF00503 45 - 130 2.5e-10 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 146
AccIII TCCGGA 1 cut(s) 536
AclWI GGATC 5 cut(s) 52, 65, 130, 207, 393
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 7 cut(s) 39, 73, 118, 146, 196, 486, 502
AgsI TTSAA 1 cut(s) 349
AjnI CCWGG 1 cut(s) 391
AluBI AGCT 2 cut(s) 53, 308
AluI AGCT 2 cut(s) 53, 308
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 2 cut(s) 133, 155
Alw44I GTGCAC 1 cut(s) 396
AlwI GGATC 5 cut(s) 52, 65, 130, 207, 393
Aor13HI TCCGGA 1 cut(s) 536
AoxI GGCC 3 cut(s) 224, 258, 493
ApaLI GTGCAC 1 cut(s) 396
ApeKI GCWGC 1 cut(s) 410
BaeGI GKGCMC 1 cut(s) 400
BamHI GGATCC 1 cut(s) 57
Bbv12I GWGCWC 1 cut(s) 400
BbvI GCAGC 1 cut(s) 397
BccI CCATC 1 cut(s) 109
BcgI CGANNNNNNTGC 2 cut(s) 305, 339
BciT130I CCWGG 1 cut(s) 393
BcoDI GTCTC 2 cut(s) 133, 155
BfaI CTAG 1 cut(s) 282
BisI GCNGC 1 cut(s) 411
BlsI GCNGC 1 cut(s) 412
Bme1390I CCNGG 1 cut(s) 393
BmiI GGNNCC 2 cut(s) 59, 110
BmrFI CCNGG 1 cut(s) 393
BmsI GCATC 2 cut(s) 227, 397
BplI GAGNNNNNCTC 2 cut(s) 113, 145
BpmI CTGGAG 2 cut(s) 95, 353
BpuEI CTTGAG 1 cut(s) 515
BsaJI CCNNGG 1 cut(s) 67
BsaWI WCCGGW 1 cut(s) 536
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bsc4I CCNNNNNNNGG 7 cut(s) 39, 73, 118, 146, 196, 486, 502
Bse1I ACTGG 3 cut(s) 336, 454, 518
BseAI TCCGGA 1 cut(s) 536
BseBI CCWGG 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 67
BseLI CCNNNNNNNGG 7 cut(s) 39, 73, 118, 146, 196, 486, 502
BseMII CTCAG 2 cut(s) 269, 411
BseNI ACTGG 3 cut(s) 336, 454, 518
BseRI GAGGAG 1 cut(s) 350
BseSI GKGCMC 1 cut(s) 400
BseXI GCAGC 1 cut(s) 397
BsgI GTGCAG 2 cut(s) 188, 375
BshFI GGCC 3 cut(s) 226, 260, 495
BsiHKAI GWGCWC 1 cut(s) 400
BsiSI CCGG 1 cut(s) 537
BslI CCNNNNNNNGG 7 cut(s) 39, 73, 118, 146, 196, 486, 502
BsmAI GTCTC 2 cut(s) 133, 155
BsnI GGCC 3 cut(s) 226, 260, 495
Bsp1286I GDGCHC 1 cut(s) 400
Bsp13I TCCGGA 1 cut(s) 536
Bsp143I GATC 4 cut(s) 57, 135, 199, 385
BspANI GGCC 3 cut(s) 226, 260, 495
BspCNI CTCAG 2 cut(s) 268, 412
BspEI TCCGGA 1 cut(s) 536
BspLI GGNNCC 2 cut(s) 59, 110
BspPI GGATC 5 cut(s) 52, 65, 130, 207, 393
BsrI ACTGG 3 cut(s) 336, 454, 518
BssECI CCNNGG 1 cut(s) 67
BssMI GATC 4 cut(s) 57, 135, 199, 385
Bst2UI CCWGG 1 cut(s) 393
Bst4CI ACNGT 1 cut(s) 166
Bst6I CTCTTC 2 cut(s) 34, 336
BstC8I GCNNGC 1 cut(s) 85
BstDEI CTNAG 2 cut(s) 255, 420
BstENI CCTNNNNNAGG 1 cut(s) 500
BstKTI GATC 4 cut(s) 60, 138, 202, 388
BstMAI GTCTC 2 cut(s) 133, 155
BstMBI GATC 4 cut(s) 57, 135, 199, 385
BstNI CCWGG 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 391
BstSLI GKGCMC 1 cut(s) 400
BstV1I GCAGC 1 cut(s) 397
BstX2I RGATCY 3 cut(s) 57, 199, 385
BstYI RGATCY 3 cut(s) 57, 199, 385
BsuRI GGCC 3 cut(s) 226, 260, 495
BtsIMutI CAGTG 2 cut(s) 171, 461
Cac8I GCNNGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 172
CviAII CATG 1 cut(s) 323
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 2 cut(s) 255, 420
DpnI GATC 4 cut(s) 59, 137, 201, 387
DpnII GATC 4 cut(s) 57, 135, 199, 385
DraI TTTAAA 1 cut(s) 469
Eam1104I CTCTTC 2 cut(s) 34, 336
EarI CTCTTC 2 cut(s) 34, 336
Eco147I AGGCCT 1 cut(s) 495
EcoNI CCTNNNNNAGG 1 cut(s) 500
EcoRII CCWGG 1 cut(s) 391
FaeI CATG 1 cut(s) 326
FaiI YATR 5 cut(s) 229, 263, 270, 324, 482
FatI CATG 1 cut(s) 322
Fnu4HI GCNGC 1 cut(s) 411
Fsp4HI GCNGC 1 cut(s) 411
FspBI CTAG 1 cut(s) 282
GluI GCNGC 1 cut(s) 411
GsuI CTGGAG 2 cut(s) 95, 353
HaeIII GGCC 3 cut(s) 226, 260, 495
HapII CCGG 1 cut(s) 537
Hin1II CATG 1 cut(s) 326
HinfI GANTC 1 cut(s) 278
HpaII CCGG 1 cut(s) 537
Hpy166II GTNNAC 3 cut(s) 15, 209, 398
Hpy188I TCNGA 2 cut(s) 57, 71
Hpy188III TCNNGA 2 cut(s) 112, 537
Hpy8I GTNNAC 3 cut(s) 15, 209, 398
HpyAV CCTTC 1 cut(s) 485
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4V TGCA 6 cut(s) 169, 326, 356, 398, 413, 436
HpyF3I CTNAG 2 cut(s) 255, 420
Hsp92II CATG 1 cut(s) 326
Kpn2I TCCGGA 1 cut(s) 536
Kzo9I GATC 4 cut(s) 57, 135, 199, 385
LmnI GCTCC 1 cut(s) 114
Lsp1109I GCAGC 1 cut(s) 397
LweI GCATC 2 cut(s) 227, 397
MaeI CTAG 1 cut(s) 282
MaeIII GTNAC 2 cut(s) 284, 521
MalI GATC 4 cut(s) 59, 137, 201, 387
MboI GATC 4 cut(s) 57, 135, 199, 385
MboII GAAGA 3 cut(s) 21, 353, 380
MflI RGATCY 3 cut(s) 57, 199, 385
MhlI GDGCHC 1 cut(s) 400
MnlI CCTC 8 cut(s) 71, 77, 115, 328, 331, 415, 496, 533
MroI TCCGGA 1 cut(s) 536
MseI TTAA 2 cut(s) 153, 468
MslI CAYNNNNRTG 1 cut(s) 20
MspI CCGG 1 cut(s) 537
MspR9I CCNGG 1 cut(s) 393
MvaI CCWGG 1 cut(s) 393
NdeII GATC 4 cut(s) 57, 135, 199, 385
NlaIII CATG 1 cut(s) 326
NlaIV GGNNCC 2 cut(s) 59, 110
NmuCI GTSAC 1 cut(s) 284
PceI AGGCCT 1 cut(s) 495
PcsI WCGNNNNNNNCGW 1 cut(s) 140
PfeI GAWTC 1 cut(s) 278
PflMI CCANNNNNTGG 1 cut(s) 146
PkrI GCNGC 1 cut(s) 412
Psp6I CCWGG 1 cut(s) 391
PspGI CCWGG 1 cut(s) 391
PspN4I GGNNCC 2 cut(s) 59, 110
PsuI RGATCY 3 cut(s) 57, 199, 385
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
RseI CAYNNNNRTG 1 cut(s) 20
SaqAI TTAA 2 cut(s) 153, 468
SatI GCNGC 1 cut(s) 411
Sau3AI GATC 4 cut(s) 57, 135, 199, 385
ScrFI CCNGG 1 cut(s) 393
SduI GDGCHC 1 cut(s) 400
SetI ASST 5 cut(s) 55, 126, 310, 355, 544
SfaNI GCATC 2 cut(s) 227, 397
SmiMI CAYNNNNRTG 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
SseBI AGGCCT 1 cut(s) 495
SspMI CTAG 1 cut(s) 282
StuI AGGCCT 1 cut(s) 495
StyD4I CCNGG 1 cut(s) 391
TaaI ACNGT 1 cut(s) 166
TatI WGTACW 1 cut(s) 171
TfiI GAWTC 1 cut(s) 278
Tru1I TTAA 2 cut(s) 153, 468
Tru9I TTAA 2 cut(s) 153, 468
TscAI CASTG 2 cut(s) 171, 461
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 1 cut(s) 410
Tsp45I GTSAC 1 cut(s) 284
TspDTI ATGAA 1 cut(s) 311
TspRI CASTG 2 cut(s) 171, 461
Van91I CCANNNNNTGG 1 cut(s) 146
VneI GTGCAC 1 cut(s) 396
XagI CCTNNNNNAGG 1 cut(s) 500
XspI CTAG 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.