MD14G1083500.v1.1

YqaJ-like viral recombinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0002455
Physical Location & Seq
Reverse (-)
9853091 .. 9857608
4518 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1083500.v1.1.491

Sequence Viewer

Length: 840 bp
ATGCTTGCCAACTGCAGACAAGTTTTCAACCCTTCTCGTTGCTCTATGAGATTTGCTTCTTCTGAAGGTTATTCTGTTCTTAATTCAAGTGATCTTCAACACTGGTTCAAAAATTGGCAAGGACTCAGAAAAAATAAACTGACAGCAAGCACTTTTGCTGCGGCGGTTGGTTTTTTTCATCGCAGAAGACTCCAGCTTTGGCTAGAGAAGATTGGAGCCCTTGAACCATTTTCGGGTAATTTGGCTACCTGTTGGAGCAATATCAAAGAAGAGGAAGCACTTGAAAGATACAAGCTGATAACAGGAAATTCCGTTTTGTTTCCTGAATTTCAAGTTTATGGTAATAGGAATGTTGGAGAGGATTGGCTGGGAGCTTCACCAGATGGGGTGATTGATAGGCTGGTATACGGATTGCCTTCACGAGGAGTACTGGAGATTAAGTGTCCATTTTTTTATGGAAATATGAAGAAGGCTACCCCTTGGTCACGAATTCCTCTGTATTGTGTTCCACAGGCTCAGGGTTTAATGGAAATACTTGACAGGGATTGGATGGATTTCTATGTTTGGACTCCTAAAGGGAGCAGCCTATTCAGGGTATATCGGGATGCAGAATACTGGGATGTTCTGAAAATAGTTCTCTCGGATTTTTGGTGGAATCATGTCCAGCCAGCGAGGGAGATCTTGAGCAAGTCTCAGATTAGAGATCCCCTCGTTGAATTAAAATCGCTCAAGCCAACACCCAGGCATGAACTGTGTAGTTATATAGTTTATGAAAGCAAATGCATTGTTGATAACTCCAAGTTATTGATGCGTGAAATCAATGGCAAACTGGTAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YqaJ

Protein Analysis

280

Amino Acids

32.41

Weight (kDa)

8.99

Isoelectric Point (pI)

33.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YqaJ PF09588 38 - 177 2.9e-16 YqaJ-like viral recombinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 405
AciI CCGC 2 cut(s) 161, 164
AclWI GGATC 1 cut(s) 698
AcsI RAATTY 3 cut(s) 307, 326, 489
AcuI CTGAAG 1 cut(s) 84
AfaI GTAC 1 cut(s) 429
AfiI CCNNNNNNNGG 3 cut(s) 233, 422, 592
AgsI TTSAA 8 cut(s) 28, 87, 98, 109, 224, 284, 332, 716
AjnI CCWGG 1 cut(s) 740
AluBI AGCT 3 cut(s) 196, 295, 374
AluI AGCT 3 cut(s) 196, 295, 374
Alw26I GTCTC 1 cut(s) 696
AlwI GGATC 1 cut(s) 698
ApeKI GCWGC 2 cut(s) 158, 582
ApoI RAATTY 3 cut(s) 307, 326, 489
ArsI GACNNNNNNTTYG 2 cut(s) 180, 212
AsuHPI GGTGA 2 cut(s) 369, 400
BanII GRGCYC 1 cut(s) 220
BauI CACGAG 1 cut(s) 420
BbsI GAAGAC 1 cut(s) 193
BbvI GCAGC 2 cut(s) 145, 594
BccI CCATC 2 cut(s) 377, 544
BciT130I CCWGG 1 cut(s) 742
BcoDI GTCTC 1 cut(s) 696
BfaI CTAG 1 cut(s) 203
BfmI CTRYAG 1 cut(s) 13
BglII AGATCT 1 cut(s) 678
BisI GCNGC 3 cut(s) 159, 162, 583
BlsI GCNGC 3 cut(s) 160, 163, 584
BmcAI AGTACT 1 cut(s) 429
Bme1390I CCNGG 1 cut(s) 742
BmiI GGNNCC 1 cut(s) 217
BmrFI CCNGG 1 cut(s) 742
BmrI ACTGGG 1 cut(s) 625
BmsI GCATC 2 cut(s) 595, 798
BmuI ACTGGG 1 cut(s) 625
BpiI GAAGAC 1 cut(s) 193
BplI GAGNNNNNCTC 4 cut(s) 676, 708, 693, 725
BpmI CTGGAG 2 cut(s) 176, 452
Bpu10I CCTNAGC 1 cut(s) 516
BpuEI CTTGAG 2 cut(s) 703, 713
BsaJI CCNNGG 2 cut(s) 479, 740
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 422, 592
Bse1I ACTGG 4 cut(s) 107, 435, 620, 834
BseBI CCWGG 1 cut(s) 742
BseDI CCNNGG 2 cut(s) 479, 740
BseGI GGATG 3 cut(s) 555, 610, 625
BseLI CCNNNNNNNGG 3 cut(s) 233, 422, 592
BseMII CTCAG 3 cut(s) 139, 530, 707
BseNI ACTGG 4 cut(s) 107, 435, 620, 834
BseRI GAGGAG 1 cut(s) 438
BseXI GCAGC 2 cut(s) 145, 594
BseYI CCCAGC 1 cut(s) 367
BslI CCNNNNNNNGG 3 cut(s) 233, 422, 592
BsmAI GTCTC 1 cut(s) 696
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 3 cut(s) 91, 678, 703
BspACI CCGC 2 cut(s) 161, 164
BspCNI CTCAG 3 cut(s) 138, 529, 706
BspLI GGNNCC 1 cut(s) 217
BspMAI CTGCAG 1 cut(s) 17
BspPI GGATC 1 cut(s) 698
BsrI ACTGG 4 cut(s) 107, 435, 620, 834
BssECI CCNNGG 2 cut(s) 479, 740
BssMI GATC 3 cut(s) 91, 678, 703
BssNAI GTATAC 1 cut(s) 406
BssSI CACGAG 1 cut(s) 420
BssT1I CCWWGG 1 cut(s) 479
Bst1107I GTATAC 1 cut(s) 406
Bst2BI CACGAG 1 cut(s) 420
Bst2UI CCWGG 1 cut(s) 742
Bst4CI ACNGT 1 cut(s) 753
Bst6I CTCTTC 1 cut(s) 264
BstC8I GCNNGC 3 cut(s) 6, 148, 669
BstDEI CTNAG 3 cut(s) 125, 516, 693
BstENI CCTNNNNNAGG 1 cut(s) 420
BstF5I GGATG 3 cut(s) 555, 610, 625
BstKTI GATC 3 cut(s) 94, 681, 706
BstMAI GTCTC 1 cut(s) 696
BstMBI GATC 3 cut(s) 91, 678, 703
BstNI CCWGG 1 cut(s) 742
BstSCI CCNGG 1 cut(s) 740
BstSFI CTRYAG 1 cut(s) 13
BstV1I GCAGC 2 cut(s) 145, 594
BstV2I GAAGAC 1 cut(s) 193
BstX2I RGATCY 2 cut(s) 678, 703
BstYI RGATCY 2 cut(s) 678, 703
BstZ17I GTATAC 1 cut(s) 406
BtgZI GCGATG 1 cut(s) 164
BtsCI GGATG 3 cut(s) 555, 610, 625
BtsIMutI CAGTG 1 cut(s) 100
Cac8I GCNNGC 3 cut(s) 6, 148, 669
Csp6I GTAC 1 cut(s) 428
CviAII CATG 2 cut(s) 659, 746
CviQI GTAC 1 cut(s) 428
DdeI CTNAG 3 cut(s) 125, 516, 693
DpnI GATC 3 cut(s) 93, 680, 705
DpnII GATC 3 cut(s) 91, 678, 703
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Eco130I CCWWGG 1 cut(s) 479
Eco24I GRGCYC 1 cut(s) 220
Eco57I CTGAAG 1 cut(s) 84
EcoNI CCTNNNNNAGG 1 cut(s) 420
EcoRI GAATTC 1 cut(s) 489
EcoRII CCWGG 1 cut(s) 740
EcoT14I CCWWGG 1 cut(s) 479
EcoT22I ATGCAT 1 cut(s) 785
EcoT38I GRGCYC 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 479
FaeI CATG 2 cut(s) 662, 749
FatI CATG 2 cut(s) 658, 745
FblI GTMKAC 1 cut(s) 405
Fnu4HI GCNGC 3 cut(s) 159, 162, 583
FokI GGATG 3 cut(s) 562, 617, 632
FriOI GRGCYC 1 cut(s) 220
Fsp4HI GCNGC 3 cut(s) 159, 162, 583
FspBI CTAG 1 cut(s) 203
GluI GCNGC 3 cut(s) 159, 162, 583
GsaI CCCAGC 1 cut(s) 371
GsuI CTGGAG 2 cut(s) 176, 452
Hin1II CATG 2 cut(s) 662, 749
HinfI GANTC 4 cut(s) 123, 189, 568, 655
HphI GGTGA 2 cut(s) 369, 400
Hpy166II GTNNAC 2 cut(s) 406, 835
Hpy188I TCNGA 5 cut(s) 64, 128, 627, 643, 696
Hpy188III TCNNGA 5 cut(s) 323, 420, 486, 602, 682
Hpy8I GTNNAC 2 cut(s) 406, 835
HpyAV CCTTC 4 cut(s) 42, 59, 426, 463
HpyCH4III ACNGT 1 cut(s) 753
HpyCH4V TGCA 3 cut(s) 15, 608, 783
HpyF3I CTNAG 3 cut(s) 125, 516, 693
Hsp92II CATG 2 cut(s) 662, 749
Kzo9I GATC 3 cut(s) 91, 678, 703
LmnI GCTCC 4 cut(s) 215, 255, 371, 579
Lsp1109I GCAGC 2 cut(s) 145, 594
LweI GCATC 2 cut(s) 595, 798
MaeI CTAG 1 cut(s) 203
MaeIII GTNAC 1 cut(s) 483
MalI GATC 3 cut(s) 93, 680, 705
MboI GATC 3 cut(s) 91, 678, 703
MboII GAAGA 6 cut(s) 51, 86, 198, 220, 281, 478
MflI RGATCY 2 cut(s) 678, 703
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 7 cut(s) 82, 112, 238, 307, 326, 489, 716
MlyI GAGTC 3 cut(s) 117, 183, 562
MmeI TCCRAC 2 cut(s) 233, 334
MnlI CCTC 6 cut(s) 265, 352, 416, 504, 666, 719
Mph1103I ATGCAT 1 cut(s) 785
MseI TTAA 4 cut(s) 81, 438, 524, 719
MspR9I CCNGG 1 cut(s) 742
MvaI CCWGG 1 cut(s) 742
NdeII GATC 3 cut(s) 91, 678, 703
NlaIII CATG 2 cut(s) 662, 749
NlaIV GGNNCC 1 cut(s) 217
NmuCI GTSAC 1 cut(s) 483
NsiI ATGCAT 1 cut(s) 785
PfeI GAWTC 1 cut(s) 655
PkrI GCNGC 3 cut(s) 160, 163, 584
PleI GAGTC 3 cut(s) 117, 183, 562
PpsI GAGTC 3 cut(s) 117, 183, 562
Psp6I CCWGG 1 cut(s) 740
PspFI CCCAGC 1 cut(s) 367
PspGI CCWGG 1 cut(s) 740
PspN4I GGNNCC 1 cut(s) 217
PstI CTGCAG 1 cut(s) 17
PsuI RGATCY 2 cut(s) 678, 703
RsaI GTAC 1 cut(s) 429
RsaNI GTAC 1 cut(s) 428
SaqAI TTAA 4 cut(s) 81, 438, 524, 719
SatI GCNGC 3 cut(s) 159, 162, 583
Sau3AI GATC 3 cut(s) 91, 678, 703
ScaI AGTACT 1 cut(s) 429
SchI GAGTC 3 cut(s) 117, 183, 562
ScrFI CCNGG 1 cut(s) 742
SduI GDGCHC 1 cut(s) 220
SetI ASST 5 cut(s) 70, 198, 251, 297, 376
SfaNI GCATC 2 cut(s) 595, 798
SfcI CTRYAG 1 cut(s) 13
SmlI CTYRAG 2 cut(s) 682, 728
SmoI CTYRAG 2 cut(s) 682, 728
Sse9I AATT 7 cut(s) 82, 112, 238, 307, 326, 489, 716
SsiI CCGC 2 cut(s) 161, 164
SspMI CTAG 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 740
StyI CCWWGG 1 cut(s) 479
TaaI ACNGT 1 cut(s) 753
TasI AATT 7 cut(s) 82, 112, 238, 307, 326, 489, 716
TatI WGTACW 1 cut(s) 427
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 1 cut(s) 655
Tru1I TTAA 4 cut(s) 81, 438, 524, 719
Tru9I TTAA 4 cut(s) 81, 438, 524, 719
TscAI CASTG 1 cut(s) 107
TseFI GTSAC 1 cut(s) 483
TseI GCWGC 2 cut(s) 158, 582
Tsp45I GTSAC 1 cut(s) 483
TspDTI ATGAA 4 cut(s) 167, 479, 762, 786
TspGWI ACGGA 2 cut(s) 301, 423
TspRI CASTG 1 cut(s) 107
XagI CCTNNNNNAGG 1 cut(s) 420
XapI RAATTY 3 cut(s) 307, 326, 489
XmiI GTMKAC 1 cut(s) 405
XspI CTAG 1 cut(s) 203
ZrmI AGTACT 1 cut(s) 429
Zsp2I ATGCAT 1 cut(s) 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.