Rorug06G0108800

YqaJ-like viral recombinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002455
Physical Location & Seq
Forward (+)
14718587 .. 14720586
2000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0108800.1

Sequence Viewer

Length: 855 bp
ATGGTGAGCATGGCATGGCCAATCATATTTGTTTTTCCACCACCAGCTTCTGTGTTCATCACAGCCATGTCAGTGATCAGCTTCGCAGGGTTAACCAATGCCGGGTTCTCGGAGGTAAGAGGAAAACACCTGCAGTATTCCAAGTTCTGGAAGACTAGCAATCAACCCGATTCTCAAAAATCAAAGCAAATTTACATATCTTCTAGGAGAGGGATGCAAATCGCTTATACTCCCGCATTTCTTGCTGGTCTCACGTCCTTGGTGTTCTTTCCCCATAACGATGATATCAGATATCTATTGCTTTCTTTAGCTCTCACCATTCATTTCCTCAAACGACTTTTGGAGGTTCAATTTGTTCACAAGTACAGTGGAGGGGGGATGCTTGATACTGTGATTCCTATCTCTCTTAGTTACTTCATGTCTACTGTGACTGCGATTTATGCTCAACACCTTACTATAACCCAAGGGCTACCGGAGCCACCAATTGATTTGAAGTATACTGGAATTCTATTGTTTCTAATTGGGATATCTGGCAATTTCTACCATCACTACCTTCTTTCCCGATTGAGATCATCATCCTCTTCATCTCCGTCAAACTCAAAAAGCAATGATGATAAGGATTATAAGATTCCAAGGGGTGGAATGTTTGAGCTAGTGGTATGCCCTCACTATCTCTTTGAAATCATAGGATTTGTAGGGTTCTCTTTTATTTCTCAGACCTTGTATGCATTCGCCTTCGTGGCCGGAACAGCATTCTATTTGATTGGTAGGAGCTATGCTACTAGGAGATGGTATCTTTCTAAGTTTAAACATTTTCCCCAAAATGTCAAGGCTCTCATTCCATATCTTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YqaJ

Protein Analysis

284

Amino Acids

32.15

Weight (kDa)

9.67

Isoelectric Point (pI)

48.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Steroid_dh PF02544 164 - 284 7.8e-25 3-oxo-5-alpha-steroid 4-dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 624
AarI CACCTGC 1 cut(s) 138
Acc36I ACCTGC 1 cut(s) 138
AccB7I CCANNNNNTGG 2 cut(s) 147, 638
AccI GTMKAC 2 cut(s) 422, 497
AciI CCGC 1 cut(s) 234
AcoI YGGCCR 2 cut(s) 17, 741
AcsI RAATTY 2 cut(s) 189, 504
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 3 cut(s) 102, 147, 638
AgsI TTSAA 3 cut(s) 350, 493, 680
AjiI CACGTC 1 cut(s) 255
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
AluBI AGCT 5 cut(s) 47, 81, 311, 652, 774
AluI AGCT 5 cut(s) 47, 81, 311, 652, 774
Alw26I GTCTC 1 cut(s) 254
AlwNI CAGNNNCTG 1 cut(s) 50
AoxI GGCC 2 cut(s) 17, 741
ApoI RAATTY 2 cut(s) 189, 504
AsuC2I CCSGG 1 cut(s) 103
AsuHPI GGTGA 2 cut(s) 16, 307
BalI TGGCCA 1 cut(s) 19
BbsI GAAGAC 1 cut(s) 158
BccI CCATC 2 cut(s) 552, 783
BclI TGATCA 1 cut(s) 75
BcnI CCSGG 1 cut(s) 103
BcoDI GTCTC 1 cut(s) 254
BfaI CTAG 4 cut(s) 156, 204, 653, 783
BfmI CTRYAG 1 cut(s) 131
BfuAI ACCTGC 1 cut(s) 138
BglI GCCNNNNNGGC 1 cut(s) 740
Bme1390I CCNGG 1 cut(s) 103
BmgBI CACGTC 1 cut(s) 255
BmiI GGNNCC 1 cut(s) 477
BmrFI CCNGG 1 cut(s) 103
BmsI GCATC 2 cut(s) 204, 369
BpiI GAAGAC 1 cut(s) 158
BpuMI CCSGG 1 cut(s) 103
BsaBI GATNNNNATC 4 cut(s) 218, 398, 568, 574
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 3 cut(s) 258, 463, 632
BsaWI WCCGGW 1 cut(s) 472
Bsc4I CCNNNNNNNGG 3 cut(s) 102, 147, 638
Bse1I ACTGG 1 cut(s) 505
Bse3DI GCAATG 1 cut(s) 613
Bse8I GATNNNNATC 4 cut(s) 218, 398, 568, 574
BseDI CCNNGG 3 cut(s) 258, 463, 632
BseGI GGATG 3 cut(s) 219, 384, 575
BseJI GATNNNNATC 4 cut(s) 218, 398, 568, 574
BseLI CCNNNNNNNGG 3 cut(s) 102, 147, 638
BseMI GCAATG 1 cut(s) 613
BseMII CTCAG 1 cut(s) 728
BseNI ACTGG 1 cut(s) 505
BshFI GGCC 2 cut(s) 19, 743
BsiSI CCGG 3 cut(s) 102, 473, 744
BslI CCNNNNNNNGG 3 cut(s) 102, 147, 638
BsmAI GTCTC 1 cut(s) 254
BsmI GAATGC 2 cut(s) 728, 752
BsnI GGCC 2 cut(s) 19, 743
Bso31I GGTCTC 1 cut(s) 254
Bsp143I GATC 2 cut(s) 75, 569
BspACI CCGC 1 cut(s) 234
BspANI GGCC 2 cut(s) 19, 743
BspCNI CTCAG 1 cut(s) 727
BspLI GGNNCC 1 cut(s) 477
BspMAI CTGCAG 1 cut(s) 135
BspMI ACCTGC 1 cut(s) 138
BspTNI GGTCTC 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 613
BsrI ACTGG 1 cut(s) 505
BssECI CCNNGG 3 cut(s) 258, 463, 632
BssMI GATC 2 cut(s) 75, 569
BssNAI GTATAC 1 cut(s) 498
BssT1I CCWWGG 3 cut(s) 258, 463, 632
Bst1107I GTATAC 1 cut(s) 498
Bst4CI ACNGT 3 cut(s) 368, 391, 427
Bst6I CTCTTC 1 cut(s) 586
BstAPI GCANNNNNTGC 1 cut(s) 242
BstDEI CTNAG 3 cut(s) 407, 714, 801
BstF5I GGATG 3 cut(s) 219, 384, 575
BstKTI GATC 2 cut(s) 78, 572
BstMAI GTCTC 1 cut(s) 254
BstMBI GATC 2 cut(s) 75, 569
BstMWI GCNNNNNNNGC 5 cut(s) 242, 440, 475, 740, 749
BstSCI CCNGG 1 cut(s) 101
BstSFI CTRYAG 1 cut(s) 131
BstV2I GAAGAC 1 cut(s) 158
BstZ17I GTATAC 1 cut(s) 498
BsuRI GGCC 2 cut(s) 19, 743
BtrI CACGTC 1 cut(s) 255
BtsCI GGATG 3 cut(s) 219, 384, 575
BtsIMutI CAGTG 2 cut(s) 78, 373
BveI ACCTGC 1 cut(s) 138
CaiI CAGNNNCTG 1 cut(s) 50
Csp6I GTAC 1 cut(s) 364
CspCI CAANNNNNGTGG 2 cut(s) 349, 384
CviAII CATG 4 cut(s) 10, 15, 67, 418
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 3 cut(s) 407, 714, 801
DpnI GATC 2 cut(s) 77, 571
DpnII GATC 2 cut(s) 75, 569
DraI TTTAAA 1 cut(s) 808
EaeI YGGCCR 2 cut(s) 17, 741
Eam1104I CTCTTC 1 cut(s) 586
EarI CTCTTC 1 cut(s) 586
Eco130I CCWWGG 3 cut(s) 258, 463, 632
Eco31I GGTCTC 1 cut(s) 254
Eco32I GATATC 3 cut(s) 286, 293, 528
EcoRI GAATTC 1 cut(s) 504
EcoRV GATATC 3 cut(s) 286, 293, 528
EcoT14I CCWWGG 3 cut(s) 258, 463, 632
EcoT22I ATGCAT 1 cut(s) 730
ErhI CCWWGG 3 cut(s) 258, 463, 632
FaeI CATG 4 cut(s) 13, 18, 70, 421
FatI CATG 4 cut(s) 9, 14, 66, 417
FauI CCCGC 1 cut(s) 241
FbaI TGATCA 1 cut(s) 75
FblI GTMKAC 2 cut(s) 422, 497
FokI GGATG 3 cut(s) 226, 391, 562
FspBI CTAG 4 cut(s) 156, 204, 653, 783
HaeIII GGCC 2 cut(s) 19, 743
HapII CCGG 3 cut(s) 102, 473, 744
Hin1II CATG 4 cut(s) 13, 18, 70, 421
HincII GTYRAC 1 cut(s) 93
HindII GTYRAC 1 cut(s) 93
HinfI GANTC 3 cut(s) 170, 394, 628
HpaI GTTAAC 1 cut(s) 93
HpaII CCGG 3 cut(s) 102, 473, 744
HphI GGTGA 2 cut(s) 16, 307
Hpy166II GTNNAC 4 cut(s) 93, 358, 423, 498
Hpy188I TCNGA 3 cut(s) 112, 290, 717
Hpy188III TCNNGA 2 cut(s) 148, 561
Hpy8I GTNNAC 4 cut(s) 93, 358, 423, 498
HpyAV CCTTC 2 cut(s) 563, 745
HpyCH4III ACNGT 3 cut(s) 368, 391, 427
HpyCH4IV ACGT 1 cut(s) 254
HpyCH4V TGCA 3 cut(s) 133, 217, 728
HpyF10VI GCNNNNNNNGC 5 cut(s) 242, 440, 475, 740, 749
HpyF3I CTNAG 3 cut(s) 407, 714, 801
HpySE526I ACGT 1 cut(s) 254
Hsp92II CATG 4 cut(s) 13, 18, 70, 421
Ksp22I TGATCA 1 cut(s) 75
KspAI GTTAAC 1 cut(s) 93
Kzo9I GATC 2 cut(s) 75, 569
LmnI GCTCC 2 cut(s) 475, 771
LweI GCATC 2 cut(s) 204, 369
MaeI CTAG 4 cut(s) 156, 204, 653, 783
MaeII ACGT 1 cut(s) 254
MaeIII GTNAC 2 cut(s) 410, 427
MalI GATC 2 cut(s) 77, 571
MboI GATC 2 cut(s) 75, 569
MboII GAAGA 3 cut(s) 163, 192, 573
MfeI CAATTG 1 cut(s) 483
MlsI TGGCCA 1 cut(s) 19
MluCI AATT 6 cut(s) 189, 350, 483, 504, 519, 535
MluNI TGGCCA 1 cut(s) 19
MnlI CCTC 8 cut(s) 106, 113, 203, 337, 338, 365, 589, 675
Mox20I TGGCCA 1 cut(s) 19
Mph1103I ATGCAT 1 cut(s) 730
MscI TGGCCA 1 cut(s) 19
MseI TTAA 2 cut(s) 92, 807
MslI CAYNNNNRTG 3 cut(s) 65, 71, 279
Msp20I TGGCCA 1 cut(s) 19
MspI CCGG 3 cut(s) 102, 473, 744
MspR9I CCNGG 1 cut(s) 103
MssI GTTTAAAC 1 cut(s) 808
MunI CAATTG 1 cut(s) 483
Mva1269I GAATGC 2 cut(s) 728, 752
MwoI GCNNNNNNNGC 5 cut(s) 242, 440, 475, 740, 749
NciI CCSGG 1 cut(s) 103
NdeII GATC 2 cut(s) 75, 569
NlaIII CATG 4 cut(s) 13, 18, 70, 421
NlaIV GGNNCC 1 cut(s) 477
NmuCI GTSAC 1 cut(s) 427
NsiI ATGCAT 1 cut(s) 730
PaqCI CACCTGC 1 cut(s) 138
PctI GAATGC 2 cut(s) 728, 752
PfeI GAWTC 3 cut(s) 170, 394, 628
PflMI CCANNNNNTGG 2 cut(s) 147, 638
PmeI GTTTAAAC 1 cut(s) 808
PsiI TTATAA 1 cut(s) 624
PspN4I GGNNCC 1 cut(s) 477
PsrI GAACNNNNNNTAC 2 cut(s) 128, 160
PstI CTGCAG 1 cut(s) 135
PstNI CAGNNNCTG 1 cut(s) 50
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 3 cut(s) 65, 71, 279
SaqAI TTAA 2 cut(s) 92, 807
Sau3AI GATC 2 cut(s) 75, 569
ScrFI CCNGG 1 cut(s) 103
SfaNI GCATC 2 cut(s) 204, 369
SfcI CTRYAG 1 cut(s) 131
SmiMI CAYNNNNRTG 3 cut(s) 65, 71, 279
Sse9I AATT 6 cut(s) 189, 350, 483, 504, 519, 535
SsiI CCGC 1 cut(s) 234
SspMI CTAG 4 cut(s) 156, 204, 653, 783
StyD4I CCNGG 1 cut(s) 101
StyI CCWWGG 3 cut(s) 258, 463, 632
TaaI ACNGT 3 cut(s) 368, 391, 427
TaiI ACGT 1 cut(s) 257
TasI AATT 6 cut(s) 189, 350, 483, 504, 519, 535
TatI WGTACW 1 cut(s) 363
TfiI GAWTC 3 cut(s) 170, 394, 628
Tru1I TTAA 2 cut(s) 92, 807
Tru9I TTAA 2 cut(s) 92, 807
TscAI CASTG 2 cut(s) 78, 373
TseFI GTSAC 1 cut(s) 427
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 4 cut(s) 46, 311, 406, 573
TspGWI ACGGA 1 cut(s) 579
TspRI CASTG 2 cut(s) 78, 373
Van91I CCANNNNNTGG 2 cut(s) 147, 638
XapI RAATTY 2 cut(s) 189, 504
XmiI GTMKAC 2 cut(s) 422, 497
XspI CTAG 4 cut(s) 156, 204, 653, 783
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.