Rorug06G0108900

YqaJ-like viral recombinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002455
Physical Location & Seq
Forward (+)
14730052 .. 14731702
1651 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0108900.1

Sequence Viewer

Length: 855 bp
ATGGTGAGCATGGCGTGGCCAATCATATTTGTTTTTCCACCACCAGCTTCTGTGTTCGTCACAGCCATGTCAGTGATCAGCTTCGCAGGGTTAGCTAATGCCGGGTTCTCGGAGGTAAGAGGAAAACACCTGCAGTACTCCAAGTTCTGGAAGACTAGCAATCAATCTGATTCTCAAAAATCAAAGCAAATTTACATATCTGGTAGAAGAGGCATGCAAATTTTGTATACTCCCGCATTTCTCGCTGGTCTCACATCCTTGGTGTTCTTTCCCCACAACGATGATATCAGATATCTATTGCTCTCTTCAGCTCTCACCATTCATTTCCTCAAACGACTTTTGGAGGTTCAATTTGTTCACAAGTACAGTGGAGGGATGGCGCTCGATTCCGCGATTCCTATCTCTCTCAGTTACTTCATGTTTACTGTGACTGCGATTTATGCTCAGCACCTTACAATAACCCAAGGGCTACCAGAGCCACAAATTGATTTGAAGTACCCTGGAATTCTGTTGTTTCTACTTGGGATATCTGGCAATTTCTACCATCACTACCTACTTTCCCAAATGAGATCATCATCCTCTTCGTCTCCGTCAAACTCAAACAGCAATGACGATAAGGATTATAAGATTCCAAGGGGTGGATTGTTTGAGCTAGTGGTATGCCCTCACTATCTCTTTGAAATCATAGGATTTGTAGGGTTCTCTTTTATTTCTCAGACCTTGTATGCATTTGCCTTCGTGGCCGGAACAGCATTCTATTTGACTGGTAGGAGCTATGCTACTAGGAGATGGTATCTTTCTAAGTTTAAACATTTTCCCCAAAATGTCAAGGCTCTCATTCCATATCTTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
YqaJ

Protein Analysis

284

Amino Acids

32.09

Weight (kDa)

9.55

Isoelectric Point (pI)

49.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Steroid_dh PF02544 148 - 284 2.9e-24 3-oxo-5-alpha-steroid 4-dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 624
AarI CACCTGC 1 cut(s) 138
Acc36I ACCTGC 1 cut(s) 138
AccB7I CCANNNNNTGG 2 cut(s) 147, 638
AccI GTMKAC 1 cut(s) 227
AccII CGCG 1 cut(s) 392
AciI CCGC 2 cut(s) 234, 390
AcoI YGGCCR 2 cut(s) 17, 741
AcsI RAATTY 3 cut(s) 189, 219, 504
AcuI CTGAAG 1 cut(s) 291
AfaI GTAC 3 cut(s) 137, 365, 497
AfiI CCNNNNNNNGG 2 cut(s) 147, 638
AgsI TTSAA 3 cut(s) 350, 493, 680
AjnI CCWGG 1 cut(s) 499
AluBI AGCT 6 cut(s) 47, 81, 95, 311, 652, 774
AluI AGCT 6 cut(s) 47, 81, 95, 311, 652, 774
Alw26I GTCTC 2 cut(s) 254, 591
AlwNI CAGNNNCTG 1 cut(s) 50
AoxI GGCC 2 cut(s) 17, 741
ApoI RAATTY 3 cut(s) 189, 219, 504
AspLEI GCGC 1 cut(s) 382
AsuC2I CCSGG 1 cut(s) 103
AsuHPI GGTGA 2 cut(s) 16, 307
BalI TGGCCA 1 cut(s) 19
BbsI GAAGAC 1 cut(s) 158
BccI CCATC 3 cut(s) 370, 552, 783
BciT130I CCWGG 1 cut(s) 501
BclI TGATCA 1 cut(s) 75
BcnI CCSGG 1 cut(s) 103
BcoDI GTCTC 2 cut(s) 254, 591
BfaI CTAG 3 cut(s) 156, 653, 783
BfmI CTRYAG 1 cut(s) 131
BfoI RGCGCY 1 cut(s) 383
BfuAI ACCTGC 1 cut(s) 138
BglI GCCNNNNNGGC 1 cut(s) 740
BlpI GCTNAGC 1 cut(s) 444
BmcAI AGTACT 1 cut(s) 137
Bme1390I CCNGG 2 cut(s) 103, 501
BmrFI CCNGG 2 cut(s) 103, 501
BpiI GAAGAC 1 cut(s) 158
Bpu1102I GCTNAGC 1 cut(s) 444
BpuMI CCSGG 1 cut(s) 103
BsaBI GATNNNNATC 2 cut(s) 398, 574
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 4 cut(s) 258, 463, 499, 632
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 638
Bse1I ACTGG 1 cut(s) 769
Bse3DI GCAATG 1 cut(s) 613
Bse8I GATNNNNATC 2 cut(s) 398, 574
BseBI CCWGG 1 cut(s) 501
BseDI CCNNGG 4 cut(s) 258, 463, 499, 632
BseGI GGATG 3 cut(s) 254, 381, 575
BseJI GATNNNNATC 2 cut(s) 398, 574
BseLI CCNNNNNNNGG 2 cut(s) 147, 638
BseMI GCAATG 1 cut(s) 613
BseMII CTCAG 3 cut(s) 421, 458, 728
BseNI ACTGG 1 cut(s) 769
Bsh1236I CGCG 1 cut(s) 392
BshFI GGCC 2 cut(s) 19, 743
BsiSI CCGG 2 cut(s) 102, 744
BslI CCNNNNNNNGG 2 cut(s) 147, 638
BsmAI GTCTC 2 cut(s) 254, 591
BsmBI CGTCTC 1 cut(s) 591
BsmI GAATGC 1 cut(s) 752
BsnI GGCC 2 cut(s) 19, 743
Bso31I GGTCTC 1 cut(s) 254
Bsp143I GATC 2 cut(s) 75, 569
Bsp1720I GCTNAGC 1 cut(s) 444
BspACI CCGC 2 cut(s) 234, 390
BspANI GGCC 2 cut(s) 19, 743
BspCNI CTCAG 3 cut(s) 420, 457, 727
BspFNI CGCG 1 cut(s) 392
BspMAI CTGCAG 1 cut(s) 135
BspMI ACCTGC 1 cut(s) 138
BspTNI GGTCTC 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 613
BsrI ACTGG 1 cut(s) 769
BssECI CCNNGG 4 cut(s) 258, 463, 499, 632
BssMI GATC 2 cut(s) 75, 569
BssNAI GTATAC 1 cut(s) 228
BssT1I CCWWGG 3 cut(s) 258, 463, 632
Bst1107I GTATAC 1 cut(s) 228
Bst2UI CCWGG 1 cut(s) 501
Bst4CI ACNGT 2 cut(s) 368, 427
Bst6I CTCTTC 3 cut(s) 202, 310, 586
BstC8I GCNNGC 1 cut(s) 215
BstDEI CTNAG 4 cut(s) 407, 444, 714, 801
BstF5I GGATG 3 cut(s) 254, 381, 575
BstFNI CGCG 1 cut(s) 392
BstH2I RGCGCY 1 cut(s) 383
BstHHI GCGC 1 cut(s) 382
BstKTI GATC 2 cut(s) 78, 572
BstMAI GTCTC 2 cut(s) 254, 591
BstMBI GATC 2 cut(s) 75, 569
BstMWI GCNNNNNNNGC 6 cut(s) 92, 242, 440, 475, 740, 749
BstNI CCWGG 1 cut(s) 501
BstNSI RCATGY 1 cut(s) 217
BstSCI CCNGG 2 cut(s) 101, 499
BstSFI CTRYAG 1 cut(s) 131
BstUI CGCG 1 cut(s) 392
BstV2I GAAGAC 1 cut(s) 158
BstZ17I GTATAC 1 cut(s) 228
BsuRI GGCC 2 cut(s) 19, 743
BtsCI GGATG 3 cut(s) 254, 381, 575
BtsIMutI CAGTG 2 cut(s) 78, 373
BveI ACCTGC 1 cut(s) 138
Cac8I GCNNGC 1 cut(s) 215
CaiI CAGNNNCTG 1 cut(s) 50
CfoI GCGC 1 cut(s) 382
Csp6I GTAC 3 cut(s) 136, 364, 496
CspCI CAANNNNNGTGG 2 cut(s) 349, 384
CviAII CATG 4 cut(s) 10, 67, 214, 418
CviQI GTAC 3 cut(s) 136, 364, 496
DdeI CTNAG 4 cut(s) 407, 444, 714, 801
DpnI GATC 2 cut(s) 77, 571
DpnII GATC 2 cut(s) 75, 569
DraI TTTAAA 1 cut(s) 808
EaeI YGGCCR 2 cut(s) 17, 741
Eam1104I CTCTTC 3 cut(s) 202, 310, 586
EarI CTCTTC 3 cut(s) 202, 310, 586
Eco130I CCWWGG 3 cut(s) 258, 463, 632
Eco31I GGTCTC 1 cut(s) 254
Eco32I GATATC 3 cut(s) 286, 293, 528
Eco57I CTGAAG 1 cut(s) 291
EcoRI GAATTC 1 cut(s) 504
EcoRII CCWGG 1 cut(s) 499
EcoRV GATATC 3 cut(s) 286, 293, 528
EcoT14I CCWWGG 3 cut(s) 258, 463, 632
EcoT22I ATGCAT 1 cut(s) 730
ErhI CCWWGG 3 cut(s) 258, 463, 632
Esp3I CGTCTC 1 cut(s) 591
FaeI CATG 4 cut(s) 13, 70, 217, 421
FatI CATG 4 cut(s) 9, 66, 213, 417
FauI CCCGC 1 cut(s) 241
FbaI TGATCA 1 cut(s) 75
FblI GTMKAC 1 cut(s) 227
FokI GGATG 3 cut(s) 241, 388, 562
FspBI CTAG 3 cut(s) 156, 653, 783
GlaI GCGC 1 cut(s) 381
HaeII RGCGCY 1 cut(s) 383
HaeIII GGCC 2 cut(s) 19, 743
HapII CCGG 2 cut(s) 102, 744
HhaI GCGC 1 cut(s) 382
Hin1II CATG 4 cut(s) 13, 70, 217, 421
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HinfI GANTC 4 cut(s) 170, 386, 394, 628
HpaII CCGG 2 cut(s) 102, 744
HphI GGTGA 2 cut(s) 16, 307
Hpy166II GTNNAC 3 cut(s) 228, 358, 423
Hpy188I TCNGA 4 cut(s) 112, 169, 290, 717
Hpy188III TCNNGA 1 cut(s) 148
Hpy8I GTNNAC 3 cut(s) 228, 358, 423
HpyAV CCTTC 1 cut(s) 745
HpyCH4III ACNGT 2 cut(s) 368, 427
HpyCH4V TGCA 3 cut(s) 133, 217, 728
HpyF10VI GCNNNNNNNGC 6 cut(s) 92, 242, 440, 475, 740, 749
HpyF3I CTNAG 4 cut(s) 407, 444, 714, 801
Hsp92II CATG 4 cut(s) 13, 70, 217, 421
HspAI GCGC 1 cut(s) 380
Ksp22I TGATCA 1 cut(s) 75
Kzo9I GATC 2 cut(s) 75, 569
LmnI GCTCC 1 cut(s) 771
MaeI CTAG 3 cut(s) 156, 653, 783
MaeIII GTNAC 3 cut(s) 58, 410, 427
MalI GATC 2 cut(s) 77, 571
MboI GATC 2 cut(s) 75, 569
MboII GAAGA 4 cut(s) 163, 219, 297, 573
MlsI TGGCCA 1 cut(s) 19
MluCI AATT 6 cut(s) 189, 219, 350, 483, 504, 535
MluNI TGGCCA 1 cut(s) 19
MnlI CCTC 8 cut(s) 106, 113, 203, 337, 338, 365, 589, 675
Mox20I TGGCCA 1 cut(s) 19
Mph1103I ATGCAT 1 cut(s) 730
MscI TGGCCA 1 cut(s) 19
MseI TTAA 1 cut(s) 807
MslI CAYNNNNRTG 3 cut(s) 65, 71, 279
Msp20I TGGCCA 1 cut(s) 19
MspI CCGG 2 cut(s) 102, 744
MspR9I CCNGG 2 cut(s) 103, 501
MssI GTTTAAAC 1 cut(s) 808
Mva1269I GAATGC 1 cut(s) 752
MvaI CCWGG 1 cut(s) 501
MvnI CGCG 1 cut(s) 392
MwoI GCNNNNNNNGC 6 cut(s) 92, 242, 440, 475, 740, 749
NciI CCSGG 1 cut(s) 103
NdeII GATC 2 cut(s) 75, 569
NlaIII CATG 4 cut(s) 13, 70, 217, 421
NmuCI GTSAC 2 cut(s) 58, 427
NsiI ATGCAT 1 cut(s) 730
NspI RCATGY 1 cut(s) 217
PaeI GCATGC 1 cut(s) 217
PaqCI CACCTGC 1 cut(s) 138
PctI GAATGC 1 cut(s) 752
PfeI GAWTC 4 cut(s) 170, 386, 394, 628
PflMI CCANNNNNTGG 2 cut(s) 147, 638
PmeI GTTTAAAC 1 cut(s) 808
PsiI TTATAA 1 cut(s) 624
Psp6I CCWGG 1 cut(s) 499
PspGI CCWGG 1 cut(s) 499
PsrI GAACNNNNNNTAC 2 cut(s) 128, 160
PstI CTGCAG 1 cut(s) 135
PstNI CAGNNNCTG 1 cut(s) 50
RsaI GTAC 3 cut(s) 137, 365, 497
RsaNI GTAC 3 cut(s) 136, 364, 496
RseI CAYNNNNRTG 3 cut(s) 65, 71, 279
SaqAI TTAA 1 cut(s) 807
Sau3AI GATC 2 cut(s) 75, 569
ScaI AGTACT 1 cut(s) 137
ScrFI CCNGG 2 cut(s) 103, 501
SfcI CTRYAG 1 cut(s) 131
SmiMI CAYNNNNRTG 3 cut(s) 65, 71, 279
SphI GCATGC 1 cut(s) 217
Sse9I AATT 6 cut(s) 189, 219, 350, 483, 504, 535
SsiI CCGC 2 cut(s) 234, 390
SspMI CTAG 3 cut(s) 156, 653, 783
StyD4I CCNGG 2 cut(s) 101, 499
StyI CCWWGG 3 cut(s) 258, 463, 632
TaaI ACNGT 2 cut(s) 368, 427
TaqI TCGA 1 cut(s) 384
TasI AATT 6 cut(s) 189, 219, 350, 483, 504, 535
TatI WGTACW 2 cut(s) 135, 363
TfiI GAWTC 4 cut(s) 170, 386, 394, 628
Tru1I TTAA 1 cut(s) 807
Tru9I TTAA 1 cut(s) 807
TscAI CASTG 2 cut(s) 78, 373
TseFI GTSAC 2 cut(s) 58, 427
Tsp45I GTSAC 2 cut(s) 58, 427
TspDTI ATGAA 2 cut(s) 311, 406
TspGWI ACGGA 1 cut(s) 579
TspRI CASTG 2 cut(s) 78, 373
Van91I CCANNNNNTGG 2 cut(s) 147, 638
XapI RAATTY 3 cut(s) 189, 219, 504
XceI RCATGY 1 cut(s) 217
XmiI GTMKAC 1 cut(s) 227
XspI CTAG 3 cut(s) 156, 653, 783
ZrmI AGTACT 1 cut(s) 137
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.