Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0002636

Physical Location & Seq

Genomic Coordinates Forward (+)

27054900 .. 27062177

Gene Structure 7278 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1177200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 933 bp

ATGATGGAGGATCTGTACGGCCTCGCCGGCTCATCGTCAACGCCTTCAGCTGGTTTCAGCTCTGATCTTTACTACTCTTCGTCAATGGCTGCTATGCCTTTCTCGGCGAATCAGACGGTTCCTGATCATCAGTTGGACTACTACTGCAACTACAGGAGCAGGAGCGCCAGCTTTGAGCCTTTAGCGGCGGCTTTGGGGGAGCAGCGGATACCCGGGACGATGAGACCGGACCAGATGTTTTCAGGGTCGTCGTCCGGAGTGTCGGACGCTGCTTCGGTGGTGGCTGCTGGGATTCAAAGAGGAGGAGGGGAAAGAGGTTGGGGAGAGGAGGTTTCTTGTCAAATGAATGAGATGATCGCTTCACATCCTCTATACCCTAAGCTTCTTCAAGCTTACATCGATTGCCAAAAGGTGGGGGCGCCACCAGAAATAGCAAGTGTGTTGGACGAAATCCGGCCACAAAGTGATCATCTAATTTCCAGGAGACCCACCGATTCAACTTGCATGGCTACTGCCGATCCTGACCTCGATCTCTTCATGGAAAGTTACTGCGATATATTGTTCAAGTACAAATCGGATCTTACGAGGCCATTCGATGAAGCAATCACCTTCTTGAACAAGATGGAGACGCAGCTCAACGCGCTCGCCAATAATACCAACGACGCTGCTGGTTCATCAGACGAAGAAGACTGGAGTGGAGGAGAGATAGAGGCTCAAGACTCTCCGCGGACAAATGAAAATCATGAGCTAAAGGATAAACTGTTGCGTAGATACAGTGGCTATATAAGCACTCTTAAACGTGAGTTTACCAAGAAGAAGAAGAATGGAAAGCTCCCTAGAGAAGCGAGGCAAATTCTCTTTGATTGGTGGAACCTTCACTATAAATGGCCTTACCCAACGGTAACTATAAATTATAACAGCTTGATTTTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009888 GO:0009889 GO:0010073 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048507 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ELK Homeobox_KN KNOX1 KNOX2

Protein Analysis

311

Amino Acids

34.65

Weight (kDa)

4.94

Isoelectric Point (pI)

50.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KNOX1	PF03790	117 - 155	2.1e-19	KNOX1 domain
KNOX2	PF03791	171 - 215	1.6e-21	KNOX2 domain
ELK	PF03789	249 - 269	4.8e-10	ELK domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002636)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23380 AT1G23380 AT1G70510 AT1G70510
fragaria_vesca	FvH4_4g26090 FvH4_4g26090 FvH4_4g26090 FvH4_4g26090 FvH4_5g05530 FvH4_5g05530
malus_domestica	MD06G1171700.v1.1 MD13G1095800.v1.1 MD14G1177200.v1.1 MD16G1097200.v1.1
prunus_persica	Prupe.1G249600_v2.0.a1 Prupe.5G171100_v2.0.a1
pyrus_communis	pycom06g15280 pycom13g08340 pycom14g14800 pycom16g08280
rosa_chinensis	RchiOBHm_Chr4g0433571 RchiOBHm_Chr7g0193251
rosa_laevigata	RLG00000004283 RLG00000006743
rosa_multiflora	Rmu_sc0017266.1_g000003
rosa_roxburghii	Rroxscaffold_3G00262250 Rroxscaffold_5G00375060
rosa_rugosa	Rorug04G0269800 Rorug07G0008500 Rorug07G0008600
rosa_samantha	Rh4AG324700 Rh4BG332400 Rh4CG347700 Rh4DG328800 Rh7AG134100 Rh7BG135100 Rh7CG138100 Rh7DG136100
rosa_wichuraiana	Rw4G028100 Rw7G011470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	915, 931
AccB1I	GGYRCC	1 cut(s)	418
AccB7I	CCANNNNNTGG	1 cut(s)	412
AccII	CGCG	2 cut(s)	641, 727
AccIII	TCCGGA	1 cut(s)	254
AciI	CCGC	5 cut(s)	185, 188, 205, 725, 727
AclWI	GGATC	3 cut(s)	18, 512, 585
AcoI	YGGCCR	1 cut(s)	455
AcsI	RAATTY	1 cut(s)	852
AcuI	CTGAAG	1 cut(s)	30
AcyI	GRCGYC	1 cut(s)	419
AdeI	CACNNNGTG	1 cut(s)	464
AfaI	GTAC	2 cut(s)	17, 569
AfiI	CCNNNNNNNGG	2 cut(s)	50, 412
AgsI	TTSAA	5 cut(s)	296, 389, 498, 565, 616
AjnI	CCWGG	1 cut(s)	479
AluBI	AGCT	9 cut(s)	50, 60, 171, 382, 392, 634, 748, 832, 921
AluI	AGCT	9 cut(s)	50, 60, 171, 382, 392, 634, 748, 832, 921
Alw26I	GTCTC	3 cut(s)	217, 478, 620
AlwI	GGATC	3 cut(s)	18, 512, 585
Ama87I	CYCGRG	1 cut(s)	212
Aor13HI	TCCGGA	1 cut(s)	254
AoxI	GGCC	4 cut(s)	19, 455, 587, 887
ApeKI	GCWGC	6 cut(s)	89, 202, 269, 284, 631, 665
ApoI	RAATTY	1 cut(s)	852
AspLEI	GCGC	3 cut(s)	167, 421, 643
AspS9I	GGNCC	1 cut(s)	229
AsuC2I	CCSGG	2 cut(s)	213, 214
AsuHPI	GGTGA	1 cut(s)	598
AvaI	CYCGRG	1 cut(s)	212
AvaII	GGWCC	1 cut(s)	229
BanI	GGYRCC	1 cut(s)	418
BbsI	GAAGAC	1 cut(s)	693
BbvI	GCAGC	6 cut(s)	76, 214, 256, 271, 643, 652
BccI	CCATC	1 cut(s)	616
BceAI	ACGGC	1 cut(s)	34
BciT130I	CCWGG	1 cut(s)	481
BciVI	GTATCC	1 cut(s)	201
BclI	TGATCA	2 cut(s)	124, 466
BcnI	CCSGG	2 cut(s)	213, 214
BcoDI	GTCTC	3 cut(s)	217, 478, 620
BfaI	CTAG	1 cut(s)	837
BfmI	CTRYAG	1 cut(s)	151
BfoI	RGCGCY	2 cut(s)	168, 422
BfuI	GTATCC	1 cut(s)	201
BglI	GCCNNNNNGGC	1 cut(s)	27
BisI	GCNGC	8 cut(s)	90, 186, 189, 203, 270, 285, 632, 666
BlsI	GCNGC	8 cut(s)	91, 187, 190, 204, 271, 286, 633, 667
Bme1390I	CCNGG	3 cut(s)	213, 214, 481
Bme18I	GGWCC	1 cut(s)	229
BmeT110I	CYCGRG	1 cut(s)	212
BmgT120I	GGNCC	1 cut(s)	229
BmiI	GGNNCC	3 cut(s)	120, 420, 872
BmrFI	CCNGG	3 cut(s)	213, 214, 481
BpiI	GAAGAC	1 cut(s)	693
BpmI	CTGGAG	1 cut(s)	712
Bpu10I	CCTNAGC	1 cut(s)	378
BpuEI	CTTGAG	1 cut(s)	699
BpuMI	CCSGG	2 cut(s)	213, 214
Bsa29I	ATCGAT	1 cut(s)	399
BsaHI	GRCGYC	1 cut(s)	419
BsaI	GGTCTC	2 cut(s)	217, 478
BsaJI	CCNNGG	2 cut(s)	212, 725
BsaWI	WCCGGW	2 cut(s)	226, 254
BsaXI	ACNNNNNCTCC	3 cut(s)	29, 315, 345
Bsc4I	CCNNNNNNNGG	2 cut(s)	50, 412
Bse118I	RCCGGY	1 cut(s)	26
Bse1I	ACTGG	1 cut(s)	695
BseAI	TCCGGA	1 cut(s)	254
BseBI	CCWGG	1 cut(s)	481
BseCI	ATCGAT	1 cut(s)	399
BseDI	CCNNGG	2 cut(s)	212, 725
BseGI	GGATG	1 cut(s)	364
BseLI	CCNNNNNNNGG	2 cut(s)	50, 412
BseNI	ACTGG	1 cut(s)	695
BseRI	GAGGAG	4 cut(s)	315, 318, 341, 714
BseXI	GCAGC	6 cut(s)	76, 214, 256, 271, 643, 652
BseYI	CCCAGC	1 cut(s)	287
Bsh1236I	CGCG	2 cut(s)	641, 727
BshFI	GGCC	4 cut(s)	21, 457, 589, 889
BshNI	GGYRCC	1 cut(s)	418
BshVI	ATCGAT	1 cut(s)	399
BsiHKCI	CYCGRG	1 cut(s)	212
BsiSI	CCGG	5 cut(s)	27, 213, 227, 255, 454
BslFI	GGGAC	1 cut(s)	229
BslI	CCNNNNNNNGG	2 cut(s)	50, 412
BsmAI	GTCTC	3 cut(s)	217, 478, 620
BsmBI	CGTCTC	1 cut(s)	620
BsmFI	GGGAC	1 cut(s)	229
BsnI	GGCC	4 cut(s)	21, 457, 589, 889
Bso31I	GGTCTC	2 cut(s)	217, 478
BsoBI	CYCGRG	1 cut(s)	212
Bsp13I	TCCGGA	1 cut(s)	254
Bsp143I	GATC	8 cut(s)	10, 64, 124, 354, 466, 517, 529, 577
BspACI	CCGC	5 cut(s)	185, 188, 205, 725, 727
BspANI	GGCC	4 cut(s)	21, 457, 589, 889
BspDI	ATCGAT	1 cut(s)	399
BspEI	TCCGGA	1 cut(s)	254
BspFNI	CGCG	2 cut(s)	641, 727
BspHI	TCATGA	1 cut(s)	742
BspLI	GGNNCC	3 cut(s)	120, 420, 872
BspPI	GGATC	3 cut(s)	18, 512, 585
BspT107I	GGYRCC	1 cut(s)	418
BspTNI	GGTCTC	2 cut(s)	217, 478
BsrFI	RCCGGY	1 cut(s)	26
BsrI	ACTGG	1 cut(s)	695
BssAI	RCCGGY	1 cut(s)	26
BssECI	CCNNGG	2 cut(s)	212, 725
BssMI	GATC	8 cut(s)	10, 64, 124, 354, 466, 517, 529, 577
BssNI	GRCGYC	1 cut(s)	419
Bst2UI	CCWGG	1 cut(s)	481
Bst4CI	ACNGT	4 cut(s)	118, 762, 776, 901
Bst6I	CTCTTC	2 cut(s)	82, 539
BstACI	GRCGYC	1 cut(s)	419
BstC8I	GCNNGC	3 cut(s)	28, 169, 645
BstDEI	CTNAG	1 cut(s)	378
BstDSI	CCRYGG	1 cut(s)	725
BstF5I	GGATG	1 cut(s)	364
BstFNI	CGCG	2 cut(s)	641, 727
BstH2I	RGCGCY	2 cut(s)	168, 422
BstHHI	GCGC	3 cut(s)	167, 421, 643
BstKTI	GATC	8 cut(s)	13, 67, 127, 357, 469, 520, 532, 580
BstMAI	GTCTC	3 cut(s)	217, 478, 620
BstMBI	GATC	8 cut(s)	10, 64, 124, 354, 466, 517, 529, 577
BstMWI	GCNNNNNNNGC	3 cut(s)	27, 640, 786
BstNI	CCWGG	1 cut(s)	481
BstSCI	CCNGG	3 cut(s)	211, 212, 479
BstSFI	CTRYAG	1 cut(s)	151
BstUI	CGCG	2 cut(s)	641, 727
BstV1I	GCAGC	6 cut(s)	76, 214, 256, 271, 643, 652
BstV2I	GAAGAC	1 cut(s)	693
BstX2I	RGATCY	2 cut(s)	10, 577
BstYI	RGATCY	2 cut(s)	10, 577
Bsu15I	ATCGAT	1 cut(s)	399
BsuI	GTATCC	1 cut(s)	201
BsuRI	GGCC	4 cut(s)	21, 457, 589, 889
BsuTUI	ATCGAT	1 cut(s)	399
BtgI	CCRYGG	1 cut(s)	725
BtsCI	GGATG	1 cut(s)	364
BtsIMutI	CAGTG	1 cut(s)	781
Cac8I	GCNNGC	3 cut(s)	28, 169, 645
CciI	TCATGA	1 cut(s)	742
CfoI	GCGC	3 cut(s)	167, 421, 643
Cfr10I	RCCGGY	1 cut(s)	26
Cfr13I	GGNCC	1 cut(s)	229
Cfr42I	CCGCGG	1 cut(s)	728
Cfr9I	CCCGGG	1 cut(s)	212
ClaI	ATCGAT	1 cut(s)	399
CseI	GACGC	3 cut(s)	275, 637, 671
Csp6I	GTAC	2 cut(s)	16, 568
CviAII	CATG	3 cut(s)	505, 538, 743
CviQI	GTAC	2 cut(s)	16, 568
DdeI	CTNAG	1 cut(s)	378
DinI	GGCGCC	1 cut(s)	420
DpnI	GATC	8 cut(s)	12, 66, 126, 356, 468, 519, 531, 579
DpnII	GATC	8 cut(s)	10, 64, 124, 354, 466, 517, 529, 577
DraIII	CACNNNGTG	1 cut(s)	464
EaeI	YGGCCR	1 cut(s)	455
Eam1104I	CTCTTC	2 cut(s)	82, 539
EarI	CTCTTC	2 cut(s)	82, 539
Eco31I	GGTCTC	2 cut(s)	217, 478
Eco47I	GGWCC	1 cut(s)	229
Eco57I	CTGAAG	1 cut(s)	30
Eco88I	CYCGRG	1 cut(s)	212
EcoRII	CCWGG	1 cut(s)	479
EgeI	GGCGCC	1 cut(s)	420
EheI	GGCGCC	1 cut(s)	420
Esp3I	CGTCTC	1 cut(s)	620
FaeI	CATG	3 cut(s)	508, 541, 746
FaqI	GGGAC	1 cut(s)	229
FatI	CATG	3 cut(s)	504, 537, 742
FbaI	TGATCA	2 cut(s)	124, 466
Fnu4HI	GCNGC	8 cut(s)	90, 186, 189, 203, 270, 285, 632, 666
FokI	GGATG	1 cut(s)	351
Fsp4HI	GCNGC	8 cut(s)	90, 186, 189, 203, 270, 285, 632, 666
FspBI	CTAG	1 cut(s)	837
GlaI	GCGC	3 cut(s)	166, 420, 642
GluI	GCNGC	8 cut(s)	90, 186, 189, 203, 270, 285, 632, 666
GsaI	CCCAGC	1 cut(s)	291
GsuI	CTGGAG	1 cut(s)	712
HaeII	RGCGCY	2 cut(s)	168, 422
HaeIII	GGCC	4 cut(s)	21, 457, 589, 889
HapII	CCGG	5 cut(s)	27, 213, 227, 255, 454
HgaI	GACGC	3 cut(s)	275, 637, 671
HhaI	GCGC	3 cut(s)	167, 421, 643
Hin1I	GRCGYC	1 cut(s)	419
Hin1II	CATG	3 cut(s)	508, 541, 746
Hin6I	GCGC	3 cut(s)	165, 419, 641
HinP1I	GCGC	3 cut(s)	165, 419, 641
HincII	GTYRAC	1 cut(s)	39
HindII	GTYRAC	1 cut(s)	39
HindIII	AAGCTT	2 cut(s)	380, 390
HinfI	GANTC	4 cut(s)	109, 292, 494, 719
HpaII	CCGG	5 cut(s)	27, 213, 227, 255, 454
HphI	GGTGA	1 cut(s)	598
Hpy166II	GTNNAC	2 cut(s)	39, 807
Hpy188I	TCNGA	5 cut(s)	64, 114, 265, 577, 679
Hpy188III	TCNNGA	6 cut(s)	122, 255, 521, 613, 716, 743
Hpy8I	GTNNAC	2 cut(s)	39, 807
Hpy99I	CGWCG	2 cut(s)	253, 665
HpyAV	CCTTC	3 cut(s)	54, 619, 884
HpyCH4III	ACNGT	4 cut(s)	118, 762, 776, 901
HpyCH4IV	ACGT	1 cut(s)	799
HpyCH4V	TGCA	2 cut(s)	147, 504
HpyF10VI	GCNNNNNNNGC	3 cut(s)	27, 640, 786
HpyF3I	CTNAG	1 cut(s)	378
HpySE526I	ACGT	1 cut(s)	799
Hsp92I	GRCGYC	1 cut(s)	419
Hsp92II	CATG	3 cut(s)	508, 541, 746
HspAI	GCGC	3 cut(s)	165, 419, 641
KasI	GGCGCC	1 cut(s)	418
Kpn2I	TCCGGA	1 cut(s)	254
KroI	GCCGGC	1 cut(s)	26
KroNI	GCCGGC	1 cut(s)	28
Ksp22I	TGATCA	2 cut(s)	124, 466
KspI	CCGCGG	1 cut(s)	728
Kzo9I	GATC	8 cut(s)	10, 64, 124, 354, 466, 517, 529, 577
LmnI	GCTCC	4 cut(s)	156, 162, 199, 837
Lsp1109I	GCAGC	6 cut(s)	76, 214, 256, 271, 643, 652
MaeI	CTAG	1 cut(s)	837
MaeII	ACGT	1 cut(s)	799
MaeIII	GTNAC	2 cut(s)	545, 901
MalI	GATC	8 cut(s)	12, 66, 126, 356, 468, 519, 531, 579
MboI	GATC	8 cut(s)	10, 64, 124, 354, 466, 517, 529, 577
MboII	GAAGA	8 cut(s)	69, 377, 526, 695, 698, 826, 829, 832
MflI	RGATCY	2 cut(s)	10, 577
MluCI	AATT	3 cut(s)	474, 852, 910
Mly113I	GGCGCC	1 cut(s)	419
MlyI	GAGTC	1 cut(s)	713
MmeI	TCCRAC	3 cut(s)	114, 243, 423
MroI	TCCGGA	1 cut(s)	254
MroNI	GCCGGC	1 cut(s)	26
MseI	TTAA	1 cut(s)	795
MspA1I	CMGCKG	3 cut(s)	50, 205, 727
MspI	CCGG	5 cut(s)	27, 213, 227, 255, 454
MspR9I	CCNGG	3 cut(s)	213, 214, 481
MvaI	CCWGG	1 cut(s)	481
MvnI	CGCG	2 cut(s)	641, 727
MwoI	GCNNNNNNNGC	3 cut(s)	27, 640, 786
NaeI	GCCGGC	1 cut(s)	28
NarI	GGCGCC	1 cut(s)	419
NciI	CCSGG	2 cut(s)	213, 214
NdeII	GATC	8 cut(s)	10, 64, 124, 354, 466, 517, 529, 577
NgoMIV	GCCGGC	1 cut(s)	26
NlaIII	CATG	3 cut(s)	508, 541, 746
NlaIV	GGNNCC	3 cut(s)	120, 420, 872
NmeAIII	GCCGAG	1 cut(s)	83
PagI	TCATGA	1 cut(s)	742
PcsI	WCGNNNNNNNCGW	1 cut(s)	581
PdiI	GCCGGC	1 cut(s)	28
PfeI	GAWTC	3 cut(s)	109, 292, 494
PflMI	CCANNNNNTGG	1 cut(s)	412
PfoI	TCCNGGA	1 cut(s)	479
PkrI	GCNGC	8 cut(s)	91, 187, 190, 204, 271, 286, 633, 667
PleI	GAGTC	1 cut(s)	713
PluTI	GGCGCC	1 cut(s)	422
PpsI	GAGTC	1 cut(s)	713
PsiI	TTATAA	2 cut(s)	915, 931
Psp6I	CCWGG	1 cut(s)	479
PspFI	CCCAGC	1 cut(s)	287
PspGI	CCWGG	1 cut(s)	479
PspN4I	GGNNCC	3 cut(s)	120, 420, 872
PspPI	GGNCC	1 cut(s)	229
PsuI	RGATCY	2 cut(s)	10, 577
PvuII	CAGCTG	1 cut(s)	50
RsaI	GTAC	2 cut(s)	17, 569
RsaNI	GTAC	2 cut(s)	16, 568
SacII	CCGCGG	1 cut(s)	728
SaqAI	TTAA	1 cut(s)	795
SatI	GCNGC	8 cut(s)	90, 186, 189, 203, 270, 285, 632, 666
Sau3AI	GATC	8 cut(s)	10, 64, 124, 354, 466, 517, 529, 577
Sau96I	GGNCC	1 cut(s)	229
SchI	GAGTC	1 cut(s)	713
ScrFI	CCNGG	3 cut(s)	213, 214, 481
SfcI	CTRYAG	1 cut(s)	151
SfoI	GGCGCC	1 cut(s)	420
Sfr303I	CCGCGG	1 cut(s)	728
SgrBI	CCGCGG	1 cut(s)	728
SinI	GGWCC	1 cut(s)	229
SmaI	CCCGGG	1 cut(s)	214
SmlI	CTYRAG	1 cut(s)	714
SmoI	CTYRAG	1 cut(s)	714
Sse9I	AATT	3 cut(s)	474, 852, 910
SsiI	CCGC	5 cut(s)	185, 188, 205, 725, 727
SspDI	GGCGCC	1 cut(s)	418
SspMI	CTAG	1 cut(s)	837
StyD4I	CCNGG	3 cut(s)	211, 212, 479
TaaI	ACNGT	4 cut(s)	118, 762, 776, 901
TaiI	ACGT	1 cut(s)	802
TaqI	TCGA	3 cut(s)	399, 528, 594
TasI	AATT	3 cut(s)	474, 852, 910
TatI	WGTACW	1 cut(s)	567
TauI	GCSGC	2 cut(s)	188, 191
TfiI	GAWTC	3 cut(s)	109, 292, 494
Tru1I	TTAA	1 cut(s)	795
Tru9I	TTAA	1 cut(s)	795
TscAI	CASTG	1 cut(s)	781
TseI	GCWGC	6 cut(s)	89, 202, 269, 284, 631, 665
TspDTI	ATGAA	5 cut(s)	359, 526, 612, 663, 750
TspMI	CCCGGG	1 cut(s)	212
TspRI	CASTG	1 cut(s)	781
Van91I	CCANNNNNTGG	1 cut(s)	412
VpaK11BI	GGWCC	1 cut(s)	229
XapI	RAATTY	1 cut(s)	852
XmaI	CCCGGG	1 cut(s)	212
XspI	CTAG	1 cut(s)	837

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.