MD14G1216100.v1.1

Sulfotransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006990
Physical Location & Seq
Reverse (-)
29977558 .. 29978529
972 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1216100.v1.1.491

Sequence Viewer

Length: 972 bp
ATGGCTTCTGTCTCTCTTGGTGTTGATCAAGAAATTATCTCAACCCTCCCTAAAGACACAGGCTGGGCAGCTGAGCATTACTACCAGTACCAGAACTTCTGGTACCCCCAGGTTTTTCTTGAAGGTGTGATTTGGGCACAACAGGATTTTAGGGCTAGGGACTCTGACGTTTTCCTGGCCACCTTTCCCAAGTGTGGTACAACATGGGTGAAGGCTCTCATGATTGCCATAAAAAACCGGAAAGACTGCGATTATTCGTCACATCCATTACTCACAAAGAACCCTCATGATATTGTGCGCTACATAGAGTTGCTTGTTCACCAAGACAATCCAGTTGAGTATCTCGACTCTATGGCCTCACCTAGATTGCTTGCAACCCACGTCCCTCACTCATCGTTGCCGAACTCCGTTTTGGATTCAGATACACGGGTTGTGTACGTTGCAAGGAACCCGAAAGATGTTCTCGTTTCTTTATGGGCATTTTCCGAAAAACTAAGGTCGAAGGTATATAGACTCCCTCCTCTCCCTCTAGAAGAAGGATTTGAGTTGTTTTGCAAAGGGGTTGCACTTGCAGGCCCGTTTTGGGATCATGTACTAGGGTACTGGGAAGCAAGCTTGAAAAACCCTAATAAGGTGCTATTTTTTAAGTTTGAGGATTTGAAAGCTGATACAGAAGGATACGTAAAGAGGTTGGCAGAGTTCATAGGATATCCATTTTCTTCAGAAGAAGAGAAAGAAGGGGCTGTGCAAGAAATAATAAAGTTTTGCAGTTTTGAGAACTTGAGTAGTTTGGAGGTGAACAAAAGTGGGACGTATCATGTGGGGCCGAAAACCGATGAAAAATTCAGCAACGATGTCTTCTTCAGACGAGGGGAGACCGGAGATTCACAGAAGCACCTAACGCCTGAGATGCTCGAGCGTCTTGACAAAATCACAGAGCAAAAGTTTGGGGCATCTAATTTGAAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sulfotransfer_1

Protein Analysis

324

Amino Acids

37.05

Weight (kDa)

5.66

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sulfotransfer_1 PF00685 54 - 318 5.2e-70 Sulfotransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 102
AccB1I GGYRCC 1 cut(s) 102
AclWI GGATC 1 cut(s) 594
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 1 cut(s) 842
AcuI CTGAAG 2 cut(s) 705, 847
AfaI GTAC 6 cut(s) 89, 104, 199, 437, 594, 602
AfiI CCNNNNNNNGG 3 cut(s) 194, 583, 631
AgsI TTSAA 4 cut(s) 122, 619, 661, 964
AjiI CACGTC 1 cut(s) 382
AjnI CCWGG 2 cut(s) 108, 174
AjuI GAANNNNNNNTTGG 2 cut(s) 395, 427
AluBI AGCT 3 cut(s) 71, 615, 665
AluI AGCT 3 cut(s) 71, 615, 665
Alw26I GTCTC 2 cut(s) 16, 869
AlwI GGATC 1 cut(s) 594
Ama87I CYCGRG 1 cut(s) 914
AoxI GGCC 4 cut(s) 177, 354, 574, 824
ApeKI GCWGC 1 cut(s) 68
ApoI RAATTY 1 cut(s) 842
Asp718I GGTACC 1 cut(s) 102
AspLEI GCGC 1 cut(s) 300
AspS9I GGNCC 2 cut(s) 575, 824
AsuHPI GGTGA 4 cut(s) 220, 311, 351, 808
AvaI CYCGRG 1 cut(s) 914
BaeGI GKGCMC 1 cut(s) 139
BaeI ACNNNNGTAYC 2 cut(s) 414, 447
BalI TGGCCA 1 cut(s) 179
BanI GGYRCC 1 cut(s) 102
BarI GAAGNNNNNNTAC 2 cut(s) 80, 112
BbsI GAAGAC 1 cut(s) 850
BbvI GCAGC 1 cut(s) 80
BciT130I CCWGG 2 cut(s) 110, 176
BciVI GTATCC 1 cut(s) 671
BclI TGATCA 1 cut(s) 25
BcoDI GTCTC 2 cut(s) 16, 869
BfaI CTAG 4 cut(s) 156, 363, 530, 596
BfuI GTATCC 1 cut(s) 671
BisI GCNGC 1 cut(s) 69
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 1 cut(s) 70
Bme1390I CCNGG 2 cut(s) 110, 176
BmeT110I CYCGRG 1 cut(s) 914
BmgBI CACGTC 1 cut(s) 382
BmgT120I GGNCC 2 cut(s) 575, 824
BmiI GGNNCC 3 cut(s) 104, 449, 825
BmrFI CCNGG 2 cut(s) 110, 176
BmrI ACTGGG 1 cut(s) 613
BmsI GCATC 2 cut(s) 900, 962
BmuI ACTGGG 1 cut(s) 613
BpiI GAAGAC 1 cut(s) 850
Bpu1102I GCTNAGC 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 802
BsaAI YACGTR 1 cut(s) 682
BsaI GGTCTC 1 cut(s) 869
BsaJI CCNNGG 1 cut(s) 108
BsaWI WCCGGW 2 cut(s) 237, 878
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 583, 631
Bse1I ACTGG 3 cut(s) 85, 332, 608
BseBI CCWGG 2 cut(s) 110, 176
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 1 cut(s) 262
BseLI CCNNNNNNNGG 3 cut(s) 194, 583, 631
BseMII CTCAG 2 cut(s) 63, 897
BseNI ACTGG 3 cut(s) 85, 332, 608
BseRI GAGGAG 1 cut(s) 510
BseSI GKGCMC 1 cut(s) 139
BseXI GCAGC 1 cut(s) 80
BseYI CCCAGC 1 cut(s) 63
BshFI GGCC 4 cut(s) 179, 356, 576, 826
BshNI GGYRCC 1 cut(s) 102
BsiHKCI CYCGRG 1 cut(s) 914
BsiSI CCGG 2 cut(s) 238, 879
BslFI GGGAC 3 cut(s) 173, 368, 823
BslI CCNNNNNNNGG 3 cut(s) 194, 583, 631
BsmAI GTCTC 2 cut(s) 16, 869
BsmFI GGGAC 3 cut(s) 173, 368, 823
BsnI GGCC 4 cut(s) 179, 356, 576, 826
Bso31I GGTCTC 1 cut(s) 869
BsoBI CYCGRG 1 cut(s) 914
Bsp1286I GDGCHC 1 cut(s) 139
Bsp143I GATC 2 cut(s) 25, 586
Bsp1720I GCTNAGC 1 cut(s) 72
BspANI GGCC 4 cut(s) 179, 356, 576, 826
BspCNI CTCAG 2 cut(s) 64, 898
BspHI TCATGA 2 cut(s) 219, 286
BspLI GGNNCC 3 cut(s) 104, 449, 825
BspPI GGATC 1 cut(s) 594
BspT107I GGYRCC 1 cut(s) 102
BspTNI GGTCTC 1 cut(s) 869
BsrI ACTGG 3 cut(s) 85, 332, 608
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 2 cut(s) 25, 586
Bst2UI CCWGG 2 cut(s) 110, 176
Bst6I CTCTTC 1 cut(s) 723
BstBAI YACGTR 1 cut(s) 682
BstC8I GCNNGC 3 cut(s) 372, 574, 613
BstDEI CTNAG 3 cut(s) 72, 494, 906
BstF5I GGATG 1 cut(s) 262
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 2 cut(s) 28, 589
BstMAI GTCTC 2 cut(s) 16, 869
BstMBI GATC 2 cut(s) 25, 586
BstMWI GCNNNNNNNGC 2 cut(s) 901, 910
BstNI CCWGG 2 cut(s) 110, 176
BstSCI CCNGG 2 cut(s) 108, 174
BstSLI GKGCMC 1 cut(s) 139
BstSNI TACGTA 1 cut(s) 682
BstV1I GCAGC 1 cut(s) 80
BstV2I GAAGAC 1 cut(s) 850
BsuI GTATCC 1 cut(s) 671
BsuRI GGCC 4 cut(s) 179, 356, 576, 826
BtrI CACGTC 1 cut(s) 382
BtsCI GGATG 1 cut(s) 262
Cac8I GCNNGC 3 cut(s) 372, 574, 613
CciI TCATGA 2 cut(s) 219, 286
CfoI GCGC 1 cut(s) 300
Cfr13I GGNCC 2 cut(s) 575, 824
CseI GACGC 1 cut(s) 908
Csp6I GTAC 6 cut(s) 88, 103, 198, 436, 593, 601
CviAII CATG 5 cut(s) 204, 220, 287, 590, 818
CviQI GTAC 6 cut(s) 88, 103, 198, 436, 593, 601
DdeI CTNAG 3 cut(s) 72, 494, 906
DpnI GATC 2 cut(s) 27, 588
DpnII GATC 2 cut(s) 25, 586
EaeI YGGCCR 1 cut(s) 177
Eam1104I CTCTTC 1 cut(s) 723
EarI CTCTTC 1 cut(s) 723
Eco105I TACGTA 1 cut(s) 682
Eco31I GGTCTC 1 cut(s) 869
Eco32I GATATC 1 cut(s) 710
Eco57I CTGAAG 2 cut(s) 705, 847
Eco88I CYCGRG 1 cut(s) 914
EcoRII CCWGG 2 cut(s) 108, 174
EcoRV GATATC 1 cut(s) 710
FaeI CATG 5 cut(s) 207, 223, 290, 593, 821
FaqI GGGAC 3 cut(s) 173, 368, 823
FatI CATG 5 cut(s) 203, 219, 286, 589, 817
FbaI TGATCA 1 cut(s) 25
Fnu4HI GCNGC 1 cut(s) 69
FokI GGATG 1 cut(s) 249
Fsp4HI GCNGC 1 cut(s) 69
FspBI CTAG 4 cut(s) 156, 363, 530, 596
GlaI GCGC 1 cut(s) 299
GluI GCNGC 1 cut(s) 69
GsaI CCCAGC 1 cut(s) 67
HaeIII GGCC 4 cut(s) 179, 356, 576, 826
HapII CCGG 2 cut(s) 238, 879
HgaI GACGC 1 cut(s) 908
HhaI GCGC 1 cut(s) 300
Hin1II CATG 5 cut(s) 207, 223, 290, 593, 821
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HindIII AAGCTT 1 cut(s) 613
HinfI GANTC 5 cut(s) 161, 347, 416, 513, 884
HpaII CCGG 2 cut(s) 238, 879
HphI GGTGA 4 cut(s) 220, 311, 351, 808
Hpy166II GTNNAC 3 cut(s) 319, 436, 799
Hpy188I TCNGA 5 cut(s) 166, 421, 487, 724, 866
Hpy188III TCNNGA 7 cut(s) 29, 119, 220, 287, 344, 530, 923
Hpy8I GTNNAC 3 cut(s) 319, 436, 799
HpyAV CCTTC 6 cut(s) 116, 205, 496, 530, 668, 731
HpyCH4IV ACGT 5 cut(s) 168, 381, 438, 681, 812
HpyCH4V TGCA 7 cut(s) 374, 443, 555, 566, 572, 748, 768
HpyF10VI GCNNNNNNNGC 2 cut(s) 901, 910
HpyF3I CTNAG 3 cut(s) 72, 494, 906
HpySE526I ACGT 5 cut(s) 168, 381, 438, 681, 812
Hsp92II CATG 5 cut(s) 207, 223, 290, 593, 821
HspAI GCGC 1 cut(s) 298
KpnI GGTACC 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 25
Kzo9I GATC 2 cut(s) 25, 586
Lsp1109I GCAGC 1 cut(s) 80
LweI GCATC 2 cut(s) 900, 962
MaeI CTAG 4 cut(s) 156, 363, 530, 596
MaeII ACGT 5 cut(s) 168, 381, 438, 681, 812
MaeIII GTNAC 1 cut(s) 258
MalI GATC 2 cut(s) 27, 588
MboI GATC 2 cut(s) 25, 586
MboII GAAGA 6 cut(s) 545, 711, 737, 740, 850, 853
MhlI GDGCHC 1 cut(s) 139
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 3 cut(s) 33, 842, 958
MluNI TGGCCA 1 cut(s) 179
MlyI GAGTC 3 cut(s) 155, 341, 507
Mox20I TGGCCA 1 cut(s) 179
MscI TGGCCA 1 cut(s) 179
MseI TTAA 1 cut(s) 645
Msp20I TGGCCA 1 cut(s) 179
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 2 cut(s) 238, 879
MspR9I CCNGG 2 cut(s) 110, 176
MvaI CCWGG 2 cut(s) 110, 176
MwoI GCNNNNNNNGC 2 cut(s) 901, 910
NdeII GATC 2 cut(s) 25, 586
NlaIII CATG 5 cut(s) 207, 223, 290, 593, 821
NlaIV GGNNCC 3 cut(s) 104, 449, 825
NmuCI GTSAC 1 cut(s) 258
PaeR7I CTCGAG 1 cut(s) 914
PagI TCATGA 2 cut(s) 219, 286
PfeI GAWTC 2 cut(s) 416, 884
PkrI GCNGC 1 cut(s) 70
PleI GAGTC 3 cut(s) 155, 341, 507
PpsI GAGTC 3 cut(s) 155, 341, 507
Ppu21I YACGTR 1 cut(s) 682
Psp6I CCWGG 2 cut(s) 108, 174
PspFI CCCAGC 1 cut(s) 63
PspGI CCWGG 2 cut(s) 108, 174
PspN4I GGNNCC 3 cut(s) 104, 449, 825
PspPI GGNCC 2 cut(s) 575, 824
PspXI VCTCGAGB 1 cut(s) 914
PsrI GAACNNNNNNTAC 2 cut(s) 86, 118
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 6 cut(s) 89, 104, 199, 437, 594, 602
RsaNI GTAC 6 cut(s) 88, 103, 198, 436, 593, 601
SaqAI TTAA 1 cut(s) 645
SatI GCNGC 1 cut(s) 69
Sau3AI GATC 2 cut(s) 25, 586
Sau96I GGNCC 2 cut(s) 575, 824
SchI GAGTC 3 cut(s) 155, 341, 507
ScrFI CCNGG 2 cut(s) 110, 176
SduI GDGCHC 1 cut(s) 139
SfaNI GCATC 2 cut(s) 900, 962
Sfr274I CTCGAG 1 cut(s) 914
SlaI CTCGAG 1 cut(s) 914
SmlI CTYRAG 2 cut(s) 781, 914
SmoI CTYRAG 2 cut(s) 781, 914
SnaBI TACGTA 1 cut(s) 682
Sse9I AATT 3 cut(s) 33, 842, 958
SspMI CTAG 4 cut(s) 156, 363, 530, 596
StyD4I CCNGG 2 cut(s) 108, 174
TaiI ACGT 5 cut(s) 171, 384, 441, 684, 815
TaqI TCGA 3 cut(s) 345, 500, 915
TasI AATT 3 cut(s) 33, 842, 958
TatI WGTACW 1 cut(s) 592
TfiI GAWTC 2 cut(s) 416, 884
Tru1I TTAA 1 cut(s) 645
Tru9I TTAA 1 cut(s) 645
TseFI GTSAC 1 cut(s) 258
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 1 cut(s) 258
TspDTI ATGAA 2 cut(s) 691, 852
TspGWI ACGGA 1 cut(s) 397
XapI RAATTY 1 cut(s) 842
XbaI TCTAGA 1 cut(s) 529
XhoI CTCGAG 1 cut(s) 914
XspI CTAG 4 cut(s) 156, 363, 530, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.